Rw4G008090

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009584
Physical Location & Seq
Reverse (-)
17482211 .. 17485273
3063 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G008090.1

Sequence Viewer

Length: 525 bp
ATGGCAGCCTCCATGCTTTTCGTCTTCTCCATATTCTCAGTCCTATTATTCAACGGAATCACGGCAGAGCCACCGCTTCTGAACGACGGGTCTCCGGAGGTTGCAGATTGGCCGATAAGACTCCAACTCCAACTTCGGTTGCTGGAGACCAAGGTTTACCTTCTAGAATCAAGCATTGATGAGAAAAACCATGAACTGAGGAAAAAGGATGAAAAAATTAGGCAGATGGAGGAGATTATTCAAGGGAAATCAAACAGCAATGCATTTTTCCAAAGTGAGACAGAGGCTGTCCAGGGAAGAGAATTTTTAGATGCTATGCAATTCACAGATGAGTTACGTGAGAAGAATCCTGAGGAGCTAGTGAATCAGGTTATTGAGGAAATCATATTGCAGAATAAGAAAAAAAATGAACTGGAAGCCCAAGCAAGGGTAGCTGATCAAAAAATCCAGGGATTGAAATTAAAAGTAAAAGATATCCATGGAGGATGGCTTTCAGTTCTACATTCAGTTGCAGTTCACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

174

Amino Acids

19.94

Weight (kDa)

5.2

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 94
AciI CCGC 1 cut(s) 74
AcoI YGGCCR 1 cut(s) 110
AcsI RAATTY 1 cut(s) 302
AfiI CCNNNNNNNGG 2 cut(s) 426, 427
AgsI TTSAA 3 cut(s) 52, 242, 457
AjnI CCWGG 2 cut(s) 291, 447
AjuI GAANNNNNNNTTGG 2 cut(s) 117, 149
AluBI AGCT 2 cut(s) 358, 434
AluI AGCT 2 cut(s) 358, 434
Alw26I GTCTC 3 cut(s) 96, 140, 272
AlwNI CAGNNNCTG 1 cut(s) 287
Aor13HI TCCGGA 1 cut(s) 94
AoxI GGCC 1 cut(s) 110
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 302
AxyI CCTNAGG 1 cut(s) 351
BbsI GAAGAC 1 cut(s) 16
BbvI GCAGC 1 cut(s) 17
BccI CCATC 2 cut(s) 220, 480
BceAI ACGGC 1 cut(s) 78
BciT130I CCWGG 2 cut(s) 293, 449
BclI TGATCA 1 cut(s) 436
BcoDI GTCTC 3 cut(s) 96, 140, 272
BfaI CTAG 2 cut(s) 164, 359
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 2 cut(s) 293, 449
BmrFI CCNGG 2 cut(s) 293, 449
BmsI GCATC 1 cut(s) 301
BpiI GAAGAC 1 cut(s) 16
BpmI CTGGAG 1 cut(s) 164
BsaAI YACGTR 1 cut(s) 338
BsaI GGTCTC 2 cut(s) 96, 140
BsaJI CCNNGG 4 cut(s) 150, 292, 448, 478
BsaWI WCCGGW 1 cut(s) 94
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bsc4I CCNNNNNNNGG 2 cut(s) 426, 427
Bse1I ACTGG 1 cut(s) 417
Bse21I CCTNAGG 1 cut(s) 351
Bse3DI GCAATG 1 cut(s) 265
BseAI TCCGGA 1 cut(s) 94
BseBI CCWGG 2 cut(s) 293, 449
BseDI CCNNGG 4 cut(s) 150, 292, 448, 478
BseGI GGATG 2 cut(s) 214, 491
BseLI CCNNNNNNNGG 2 cut(s) 426, 427
BseMI GCAATG 1 cut(s) 265
BseMII CTCAG 3 cut(s) 51, 188, 342
BseNI ACTGG 1 cut(s) 417
BseRI GAGGAG 2 cut(s) 245, 368
BseXI GCAGC 1 cut(s) 17
BshFI GGCC 1 cut(s) 112
BsiSI CCGG 1 cut(s) 95
BslI CCNNNNNNNGG 2 cut(s) 426, 427
BsmAI GTCTC 3 cut(s) 96, 140, 272
BsnI GGCC 1 cut(s) 112
Bso31I GGTCTC 2 cut(s) 96, 140
Bsp13I TCCGGA 1 cut(s) 94
Bsp143I GATC 1 cut(s) 436
Bsp19I CCATGG 1 cut(s) 478
BspACI CCGC 1 cut(s) 74
BspANI GGCC 1 cut(s) 112
BspCNI CTCAG 3 cut(s) 50, 189, 343
BspEI TCCGGA 1 cut(s) 94
BspTNI GGTCTC 2 cut(s) 96, 140
BsrDI GCAATG 1 cut(s) 265
BsrI ACTGG 1 cut(s) 417
BssECI CCNNGG 4 cut(s) 150, 292, 448, 478
BssMI GATC 1 cut(s) 436
BssT1I CCWWGG 2 cut(s) 150, 478
Bst2UI CCWGG 2 cut(s) 293, 449
Bst6I CTCTTC 1 cut(s) 292
BstBAI YACGTR 1 cut(s) 338
BstDEI CTNAG 3 cut(s) 37, 197, 351
BstDSI CCRYGG 1 cut(s) 478
BstF5I GGATG 2 cut(s) 214, 491
BstKTI GATC 1 cut(s) 439
BstMAI GTCTC 3 cut(s) 96, 140, 272
BstMBI GATC 1 cut(s) 436
BstMWI GCNNNNNNNGC 1 cut(s) 431
BstNI CCWGG 2 cut(s) 293, 449
BstSCI CCNGG 2 cut(s) 291, 447
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 16
Bsu36I CCTNAGG 1 cut(s) 351
BsuRI GGCC 1 cut(s) 112
BtgI CCRYGG 1 cut(s) 478
BtsCI GGATG 2 cut(s) 214, 491
CaiI CAGNNNCTG 1 cut(s) 287
CviAII CATG 3 cut(s) 13, 191, 479
CviJI RGCY 8 cut(s) 8, 70, 112, 287, 358, 419, 434, 490
CviKI_1 RGCY 8 cut(s) 8, 70, 112, 287, 358, 419, 434, 490
DdeI CTNAG 3 cut(s) 37, 197, 351
DpnI GATC 1 cut(s) 438
DpnII GATC 1 cut(s) 436
EaeI YGGCCR 1 cut(s) 110
Eam1104I CTCTTC 1 cut(s) 292
EarI CTCTTC 1 cut(s) 292
Eco130I CCWWGG 2 cut(s) 150, 478
Eco31I GGTCTC 2 cut(s) 96, 140
Eco32I GATATC 1 cut(s) 475
Eco81I CCTNAGG 1 cut(s) 351
EcoRII CCWGG 2 cut(s) 291, 447
EcoRV GATATC 1 cut(s) 475
EcoT14I CCWWGG 2 cut(s) 150, 478
EcoT22I ATGCAT 1 cut(s) 265
ErhI CCWWGG 2 cut(s) 150, 478
FaeI CATG 3 cut(s) 16, 194, 482
FaiI YATR 8 cut(s) 14, 32, 192, 317, 386, 480, 521, 523
FatI CATG 3 cut(s) 12, 190, 478
FbaI TGATCA 1 cut(s) 436
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 2 cut(s) 221, 498
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 2 cut(s) 164, 359
GluI GCNGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 164
HaeIII GGCC 1 cut(s) 112
HapII CCGG 1 cut(s) 95
Hin1II CATG 3 cut(s) 16, 194, 482
HinfI GANTC 5 cut(s) 57, 120, 167, 346, 364
HpaII CCGG 1 cut(s) 95
Hpy166II GTNNAC 2 cut(s) 157, 517
Hpy188I TCNGA 1 cut(s) 81
Hpy188III TCNNGA 3 cut(s) 95, 164, 350
Hpy8I GTNNAC 2 cut(s) 157, 517
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 1 cut(s) 170
HpyCH4IV ACGT 1 cut(s) 337
HpyCH4V TGCA 5 cut(s) 104, 263, 319, 391, 512
HpyF10VI GCNNNNNNNGC 1 cut(s) 431
HpyF3I CTNAG 3 cut(s) 37, 197, 351
HpySE526I ACGT 1 cut(s) 337
Hsp92II CATG 3 cut(s) 16, 194, 482
Kpn2I TCCGGA 1 cut(s) 94
Ksp22I TGATCA 1 cut(s) 436
Kzo9I GATC 1 cut(s) 436
LmnI GCTCC 1 cut(s) 355
LpnPI CCDG 9 cut(s) 108, 128, 278, 305, 353, 363, 398, 434, 461
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 301
MaeI CTAG 2 cut(s) 164, 359
MaeII ACGT 1 cut(s) 337
MaeIII GTNAC 1 cut(s) 333
MalI GATC 1 cut(s) 438
MboI GATC 1 cut(s) 436
MboII GAAGA 3 cut(s) 16, 309, 355
MluCI AATT 4 cut(s) 216, 302, 320, 458
MlyI GAGTC 1 cut(s) 114
MmeI TCCRAC 2 cut(s) 148, 154
MnlI CCTC 8 cut(s) 19, 91, 192, 223, 277, 346, 370, 476
Mph1103I ATGCAT 1 cut(s) 265
MroI TCCGGA 1 cut(s) 94
MseI TTAA 1 cut(s) 461
MspI CCGG 1 cut(s) 95
MspR9I CCNGG 2 cut(s) 293, 449
MvaI CCWGG 2 cut(s) 293, 449
MwoI GCNNNNNNNGC 1 cut(s) 431
NcoI CCATGG 1 cut(s) 478
NdeII GATC 1 cut(s) 436
NlaIII CATG 3 cut(s) 16, 194, 482
NsiI ATGCAT 1 cut(s) 265
PfeI GAWTC 4 cut(s) 57, 167, 346, 364
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 114
PpsI GAGTC 1 cut(s) 114
Ppu21I YACGTR 1 cut(s) 338
Psp6I CCWGG 2 cut(s) 291, 447
PspGI CCWGG 2 cut(s) 291, 447
PstNI CAGNNNCTG 1 cut(s) 287
SaqAI TTAA 1 cut(s) 461
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 1 cut(s) 436
SchI GAGTC 1 cut(s) 114
ScrFI CCNGG 2 cut(s) 293, 449
SetI ASST 7 cut(s) 102, 156, 162, 340, 360, 372, 436
SfaNI GCATC 1 cut(s) 301
Sse9I AATT 4 cut(s) 216, 302, 320, 458
SsiI CCGC 1 cut(s) 74
SspMI CTAG 2 cut(s) 164, 359
StyD4I CCNGG 2 cut(s) 291, 447
StyI CCWWGG 2 cut(s) 150, 478
TaiI ACGT 1 cut(s) 340
TasI AATT 4 cut(s) 216, 302, 320, 458
TfiI GAWTC 4 cut(s) 57, 167, 346, 364
Tru1I TTAA 1 cut(s) 461
Tru9I TTAA 1 cut(s) 461
TseI GCWGC 1 cut(s) 5
TspDTI ATGAA 3 cut(s) 207, 225, 423
TspGWI ACGGA 1 cut(s) 69
XapI RAATTY 1 cut(s) 302
XbaI TCTAGA 1 cut(s) 163
XspI CTAG 2 cut(s) 164, 359
Zsp2I ATGCAT 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.