FvH4_4g17491

Monothiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003089
Physical Location & Seq
Forward (+)
21444324 .. 21445160
837 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17491.t1

Sequence Viewer

Length: 366 bp
ATGAAGCAACATACACCAAATGAGAAACAAAAACGAAGAACAAAATGCATATATGGCAGTAAGGCGGTGACAAAGATGGCGTCCGAGAGACCAGTGGTGATCTTCAGTAAGAGCTCATGCTGCATGAGCCACTCAATCAAGACCTTGCTTTCCGACTTTGGGGTGAACCCAGCAGTGTATGAGCTTGATGAGATGCAAAGAGGGAGAGAAATAGAGCAAGTTCTGTTGAGGCTCGGATGCAGCCCATCTGTGCCGGCTGTGTTCATTGGTGGCGAATTTGTTGGCGGAGCCAATGAAGTGATGAGTCTTCATCTTAAGCGCTCCTTAATCCCCATGCTTAAGCGCGTTGGTGCATTATGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

122

Amino Acids

13.46

Weight (kDa)

9.65

Isoelectric Point (pI)

62.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 32 - 94 7e-15 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 345
AciI CCGC 2 cut(s) 65, 285
AcsI RAATTY 1 cut(s) 275
AcuI CTGAAG 1 cut(s) 88
AcyI GRCGYC 1 cut(s) 80
AfeI AGCGCT 1 cut(s) 320
AfiI CCNNNNNNNGG 1 cut(s) 159
AflII CTTAAG 2 cut(s) 314, 338
AluBI AGCT 2 cut(s) 114, 184
AluI AGCT 2 cut(s) 114, 184
Alw21I GWGCWC 1 cut(s) 116
Alw26I GTCTC 1 cut(s) 82
Aor51HI AGCGCT 1 cut(s) 320
ApeKI GCWGC 2 cut(s) 120, 240
ApoI RAATTY 1 cut(s) 275
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
AspLEI GCGC 2 cut(s) 321, 345
AsuHPI GGTGA 3 cut(s) 79, 109, 175
BanII GRGCYC 1 cut(s) 116
BbsI GAAGAC 1 cut(s) 299
Bbv12I GWGCWC 1 cut(s) 116
BbvI GCAGC 2 cut(s) 107, 252
BccI CCATC 2 cut(s) 70, 253
BcoDI GTCTC 1 cut(s) 82
BfoI RGCGCY 1 cut(s) 322
BfrI CTTAAG 2 cut(s) 314, 338
BisI GCNGC 2 cut(s) 121, 241
BlsI GCNGC 2 cut(s) 122, 242
BmiI GGNNCC 1 cut(s) 289
BmsI GCATC 2 cut(s) 183, 227
BpiI GAAGAC 1 cut(s) 299
BsaHI GRCGYC 1 cut(s) 80
BsaI GGTCTC 1 cut(s) 82
Bsc4I CCNNNNNNNGG 1 cut(s) 159
Bse118I RCCGGY 1 cut(s) 253
Bse1I ACTGG 1 cut(s) 92
BseGI GGATG 1 cut(s) 242
BseLI CCNNNNNNNGG 1 cut(s) 159
BseNI ACTGG 1 cut(s) 92
BseXI GCAGC 2 cut(s) 107, 252
BseYI CCCAGC 1 cut(s) 169
Bsh1236I CGCG 1 cut(s) 345
BsiHKAI GWGCWC 1 cut(s) 116
BsiSI CCGG 1 cut(s) 254
BslI CCNNNNNNNGG 1 cut(s) 159
BsmAI GTCTC 1 cut(s) 82
Bso31I GGTCTC 1 cut(s) 82
Bsp1286I GDGCHC 1 cut(s) 116
Bsp143I GATC 1 cut(s) 99
BspACI CCGC 2 cut(s) 65, 285
BspFNI CGCG 1 cut(s) 345
BspLI GGNNCC 1 cut(s) 289
BspTI CTTAAG 2 cut(s) 314, 338
BspTNI GGTCTC 1 cut(s) 82
BsrFI RCCGGY 1 cut(s) 253
BsrI ACTGG 1 cut(s) 92
BssAI RCCGGY 1 cut(s) 253
BssMI GATC 1 cut(s) 99
BssNI GRCGYC 1 cut(s) 80
BstACI GRCGYC 1 cut(s) 80
BstAFI CTTAAG 2 cut(s) 314, 338
BstC8I GCNNGC 1 cut(s) 255
BstF5I GGATG 1 cut(s) 242
BstFNI CGCG 1 cut(s) 345
BstH2I RGCGCY 1 cut(s) 322
BstHHI GCGC 2 cut(s) 321, 345
BstKTI GATC 1 cut(s) 102
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 1 cut(s) 99
BstMWI GCNNNNNNNGC 3 cut(s) 54, 120, 126
BstUI CGCG 1 cut(s) 345
BstV1I GCAGC 2 cut(s) 107, 252
BstV2I GAAGAC 1 cut(s) 299
BtsCI GGATG 1 cut(s) 242
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 2 cut(s) 99, 180
Cac8I GCNNGC 1 cut(s) 255
CfoI GCGC 2 cut(s) 321, 345
Cfr10I RCCGGY 1 cut(s) 253
CseI GACGC 1 cut(s) 69
CviAII CATG 3 cut(s) 117, 124, 334
CviJI RGCY 7 cut(s) 114, 129, 184, 232, 243, 257, 290
CviKI_1 RGCY 7 cut(s) 114, 129, 184, 232, 243, 257, 290
DpnI GATC 1 cut(s) 101
DpnII GATC 1 cut(s) 99
EciI GGCGGA 1 cut(s) 300
Ecl136II GAGCTC 1 cut(s) 114
Eco24I GRGCYC 1 cut(s) 116
Eco31I GGTCTC 1 cut(s) 82
Eco47III AGCGCT 1 cut(s) 320
Eco53kI GAGCTC 1 cut(s) 114
Eco57I CTGAAG 1 cut(s) 88
EcoICRI GAGCTC 1 cut(s) 114
EcoT22I ATGCAT 1 cut(s) 50
EcoT38I GRGCYC 1 cut(s) 116
FaeI CATG 3 cut(s) 120, 127, 337
FaiI YATR 9 cut(s) 12, 50, 52, 54, 118, 125, 180, 335, 358
FalI AAGNNNNNCTT 2 cut(s) 308, 340
FatI CATG 3 cut(s) 116, 123, 333
Fnu4HI GCNGC 2 cut(s) 121, 241
FokI GGATG 1 cut(s) 249
FriOI GRGCYC 1 cut(s) 116
Fsp4HI GCNGC 2 cut(s) 121, 241
GlaI GCGC 2 cut(s) 320, 344
GluI GCNGC 2 cut(s) 121, 241
GsaI CCCAGC 1 cut(s) 173
HaeII RGCGCY 1 cut(s) 322
HapII CCGG 1 cut(s) 254
HgaI GACGC 1 cut(s) 69
HhaI GCGC 2 cut(s) 321, 345
Hin1I GRCGYC 1 cut(s) 80
Hin1II CATG 3 cut(s) 120, 127, 337
Hin6I GCGC 2 cut(s) 319, 343
HinP1I GCGC 2 cut(s) 319, 343
HinfI GANTC 1 cut(s) 304
HpaII CCGG 1 cut(s) 254
HphI GGTGA 3 cut(s) 79, 109, 175
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 3 cut(s) 85, 154, 236
Hpy188III TCNNGA 1 cut(s) 139
Hpy8I GTNNAC 1 cut(s) 166
HpyCH4V TGCA 5 cut(s) 48, 123, 196, 240, 353
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 120, 126
Hsp92I GRCGYC 1 cut(s) 80
Hsp92II CATG 3 cut(s) 120, 127, 337
HspAI GCGC 2 cut(s) 319, 343
KroI GCCGGC 1 cut(s) 253
KroNI GCCGGC 1 cut(s) 255
Kzo9I GATC 1 cut(s) 99
LmnI GCTCC 2 cut(s) 287, 326
LpnPI CCDG 3 cut(s) 105, 183, 267
Lsp1109I GCAGC 2 cut(s) 107, 252
LweI GCATC 2 cut(s) 183, 227
MaeIII GTNAC 1 cut(s) 67
MalI GATC 1 cut(s) 101
MboI GATC 1 cut(s) 99
MboII GAAGA 3 cut(s) 48, 94, 299
MhlI GDGCHC 1 cut(s) 116
MluCI AATT 1 cut(s) 275
MlyI GAGTC 1 cut(s) 313
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 2 cut(s) 194, 222
Mph1103I ATGCAT 1 cut(s) 50
MroNI GCCGGC 1 cut(s) 253
MseI TTAA 3 cut(s) 315, 326, 339
MspCI CTTAAG 2 cut(s) 314, 338
MspI CCGG 1 cut(s) 254
MvnI CGCG 1 cut(s) 345
MwoI GCNNNNNNNGC 3 cut(s) 54, 120, 126
NaeI GCCGGC 1 cut(s) 255
NdeII GATC 1 cut(s) 99
NgoMIV GCCGGC 1 cut(s) 253
NlaIII CATG 3 cut(s) 120, 127, 337
NlaIV GGNNCC 1 cut(s) 289
NmuCI GTSAC 1 cut(s) 67
NsiI ATGCAT 1 cut(s) 50
PdiI GCCGGC 1 cut(s) 255
PkrI GCNGC 2 cut(s) 122, 242
PleI GAGTC 1 cut(s) 312
PpsI GAGTC 1 cut(s) 312
Psp124BI GAGCTC 1 cut(s) 116
PspFI CCCAGC 1 cut(s) 169
PspN4I GGNNCC 1 cut(s) 289
SacI GAGCTC 1 cut(s) 116
SaqAI TTAA 3 cut(s) 315, 326, 339
SatI GCNGC 2 cut(s) 121, 241
Sau3AI GATC 1 cut(s) 99
SchI GAGTC 1 cut(s) 313
SduI GDGCHC 1 cut(s) 116
SetI ASST 3 cut(s) 116, 146, 186
SfaNI GCATC 2 cut(s) 183, 227
SmlI CTYRAG 2 cut(s) 314, 338
SmoI CTYRAG 2 cut(s) 314, 338
Sse9I AATT 1 cut(s) 275
SsiI CCGC 2 cut(s) 65, 285
SstI GAGCTC 1 cut(s) 116
TasI AATT 1 cut(s) 275
Tru1I TTAA 3 cut(s) 315, 326, 339
Tru9I TTAA 3 cut(s) 315, 326, 339
TscAI CASTG 2 cut(s) 99, 180
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 2 cut(s) 120, 240
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 4 cut(s) 17, 253, 299, 309
TspRI CASTG 2 cut(s) 99, 180
Vha464I CTTAAG 2 cut(s) 314, 338
XapI RAATTY 1 cut(s) 275
Zsp2I ATGCAT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.