Rroxscaffold_5G00363660

Monothiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003089
Physical Location & Seq
Forward (+)
44949250 .. 44949558
309 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_5G00363660.1

Sequence Viewer

Length: 309 bp
ATGGAGGCGGTAACAAACATGGCGTCCGAGAGACCAGTGGTGATCTTCAGCAAGAGCTCATGCTGCATGAGCCACTCCATCAAGACCTTGCTTTGCGACTTTGGGGTGAACCCAGCAGTGTATGAGCTGGATGAGATGCAAAGAGGTAGAGAAATAGAGCAAGCTCTGTCGAGGCTTGGATGCAATCCATCTGTGCCGGCTGTGTTCATTGGTGGTGAATTTGTGGGCGGGGCCAATGAGGTCATGAGTCTTCATCTTAACCGCTCCTTAATCCCCATGCTTAAGCGCGTTGGCGCATTGTGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

102

Amino Acids

11.14

Weight (kDa)

6.07

Isoelectric Point (pI)

51.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 13 - 75 3.8e-15 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 264
AccII CGCG 1 cut(s) 288
AciI CCGC 3 cut(s) 8, 228, 262
AcsI RAATTY 1 cut(s) 218
AcuI CTGAAG 1 cut(s) 31
AcyI GRCGYC 1 cut(s) 23
AflII CTTAAG 1 cut(s) 281
AluBI AGCT 3 cut(s) 57, 127, 164
AluI AGCT 3 cut(s) 57, 127, 164
Alw21I GWGCWC 1 cut(s) 59
Alw26I GTCTC 1 cut(s) 25
AoxI GGCC 1 cut(s) 231
ApeKI GCWGC 1 cut(s) 63
ApoI RAATTY 1 cut(s) 218
ArsI GACNNNNNNTTYG 2 cut(s) 8, 40
AspLEI GCGC 2 cut(s) 288, 296
AspS9I GGNCC 1 cut(s) 231
AsuHPI GGTGA 3 cut(s) 52, 118, 227
BanII GRGCYC 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 242
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 1 cut(s) 50
BccI CCATC 2 cut(s) 86, 196
BcoDI GTCTC 1 cut(s) 25
BfrI CTTAAG 1 cut(s) 281
BisI GCNGC 1 cut(s) 64
BlsI GCNGC 1 cut(s) 65
BmgT120I GGNCC 1 cut(s) 231
BmiI GGNNCC 1 cut(s) 232
BmsI GCATC 2 cut(s) 126, 170
BpiI GAAGAC 1 cut(s) 242
BsaHI GRCGYC 1 cut(s) 23
BsaI GGTCTC 1 cut(s) 25
Bse118I RCCGGY 1 cut(s) 196
Bse1I ACTGG 1 cut(s) 35
BseGI GGATG 2 cut(s) 136, 185
BseNI ACTGG 1 cut(s) 35
BseXI GCAGC 1 cut(s) 50
BseYI CCCAGC 1 cut(s) 112
Bsh1236I CGCG 1 cut(s) 288
BshFI GGCC 1 cut(s) 233
BsiHKAI GWGCWC 1 cut(s) 59
BsiSI CCGG 1 cut(s) 197
BsmAI GTCTC 1 cut(s) 25
BsnI GGCC 1 cut(s) 233
Bso31I GGTCTC 1 cut(s) 25
Bsp1286I GDGCHC 1 cut(s) 59
Bsp143I GATC 1 cut(s) 42
BspACI CCGC 3 cut(s) 8, 228, 262
BspANI GGCC 1 cut(s) 233
BspFNI CGCG 1 cut(s) 288
BspHI TCATGA 1 cut(s) 243
BspLI GGNNCC 1 cut(s) 232
BspTI CTTAAG 1 cut(s) 281
BspTNI GGTCTC 1 cut(s) 25
BsrBI CCGCTC 1 cut(s) 264
BsrFI RCCGGY 1 cut(s) 196
BsrI ACTGG 1 cut(s) 35
BssAI RCCGGY 1 cut(s) 196
BssMI GATC 1 cut(s) 42
BssNI GRCGYC 1 cut(s) 23
BstACI GRCGYC 1 cut(s) 23
BstAFI CTTAAG 1 cut(s) 281
BstC8I GCNNGC 2 cut(s) 162, 198
BstF5I GGATG 2 cut(s) 136, 185
BstFNI CGCG 1 cut(s) 288
BstHHI GCGC 2 cut(s) 288, 296
BstKTI GATC 1 cut(s) 45
BstMAI GTCTC 1 cut(s) 25
BstMBI GATC 1 cut(s) 42
BstMWI GCNNNNNNNGC 2 cut(s) 63, 69
BstUI CGCG 1 cut(s) 288
BstV1I GCAGC 1 cut(s) 50
BstV2I GAAGAC 1 cut(s) 242
BsuRI GGCC 1 cut(s) 233
BtsCI GGATG 2 cut(s) 136, 185
BtsI GCAGTG 1 cut(s) 123
BtsIMutI CAGTG 2 cut(s) 42, 123
Cac8I GCNNGC 2 cut(s) 162, 198
CciI TCATGA 1 cut(s) 243
CfoI GCGC 2 cut(s) 288, 296
Cfr10I RCCGGY 1 cut(s) 196
Cfr13I GGNCC 1 cut(s) 231
CseI GACGC 1 cut(s) 12
CviAII CATG 5 cut(s) 19, 60, 67, 244, 277
CviJI RGCY 7 cut(s) 57, 72, 127, 164, 175, 200, 233
CviKI_1 RGCY 7 cut(s) 57, 72, 127, 164, 175, 200, 233
DpnI GATC 1 cut(s) 44
DpnII GATC 1 cut(s) 42
Ecl136II GAGCTC 1 cut(s) 57
Eco24I GRGCYC 1 cut(s) 59
Eco31I GGTCTC 1 cut(s) 25
Eco53kI GAGCTC 1 cut(s) 57
Eco57I CTGAAG 1 cut(s) 31
EcoICRI GAGCTC 1 cut(s) 57
EcoT38I GRGCYC 1 cut(s) 59
FaeI CATG 5 cut(s) 22, 63, 70, 247, 280
FaiI YATR 6 cut(s) 20, 61, 68, 123, 245, 278
FatI CATG 5 cut(s) 18, 59, 66, 243, 276
FauI CCCGC 1 cut(s) 221
Fnu4HI GCNGC 1 cut(s) 64
FokI GGATG 2 cut(s) 143, 192
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 1 cut(s) 64
GlaI GCGC 2 cut(s) 287, 295
GluI GCNGC 1 cut(s) 64
GsaI CCCAGC 1 cut(s) 116
HaeIII GGCC 1 cut(s) 233
HapII CCGG 1 cut(s) 197
HgaI GACGC 1 cut(s) 12
HhaI GCGC 2 cut(s) 288, 296
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 5 cut(s) 22, 63, 70, 247, 280
Hin6I GCGC 2 cut(s) 286, 294
HinP1I GCGC 2 cut(s) 286, 294
HinfI GANTC 1 cut(s) 247
HpaII CCGG 1 cut(s) 197
HphI GGTGA 3 cut(s) 52, 118, 227
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 1 cut(s) 28
Hpy188III TCNNGA 2 cut(s) 82, 244
Hpy8I GTNNAC 1 cut(s) 109
HpyCH4V TGCA 3 cut(s) 66, 139, 183
HpyF10VI GCNNNNNNNGC 2 cut(s) 63, 69
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 5 cut(s) 22, 63, 70, 247, 280
HspAI GCGC 2 cut(s) 286, 294
KroI GCCGGC 1 cut(s) 196
KroNI GCCGGC 1 cut(s) 198
Kzo9I GATC 1 cut(s) 42
LmnI GCTCC 1 cut(s) 269
LpnPI CCDG 4 cut(s) 48, 113, 126, 210
Lsp1109I GCAGC 1 cut(s) 50
LweI GCATC 2 cut(s) 126, 170
MaeIII GTNAC 1 cut(s) 10
MalI GATC 1 cut(s) 44
MbiI CCGCTC 1 cut(s) 264
MboI GATC 1 cut(s) 42
MboII GAAGA 2 cut(s) 37, 242
MhlI GDGCHC 1 cut(s) 59
MluCI AATT 1 cut(s) 218
MlyI GAGTC 1 cut(s) 256
MnlI CCTC 3 cut(s) 137, 165, 232
MroNI GCCGGC 1 cut(s) 196
MseI TTAA 3 cut(s) 258, 269, 282
MspCI CTTAAG 1 cut(s) 281
MspI CCGG 1 cut(s) 197
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 2 cut(s) 63, 69
NaeI GCCGGC 1 cut(s) 198
NdeII GATC 1 cut(s) 42
NgoMIV GCCGGC 1 cut(s) 196
NlaIII CATG 5 cut(s) 22, 63, 70, 247, 280
NlaIV GGNNCC 1 cut(s) 232
PagI TCATGA 1 cut(s) 243
PdiI GCCGGC 1 cut(s) 198
PkrI GCNGC 1 cut(s) 65
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
Psp124BI GAGCTC 1 cut(s) 59
PspFI CCCAGC 1 cut(s) 112
PspN4I GGNNCC 1 cut(s) 232
PspPI GGNCC 1 cut(s) 231
SacI GAGCTC 1 cut(s) 59
SaqAI TTAA 3 cut(s) 258, 269, 282
SatI GCNGC 1 cut(s) 64
Sau3AI GATC 1 cut(s) 42
Sau96I GGNCC 1 cut(s) 231
SchI GAGTC 1 cut(s) 256
SduI GDGCHC 1 cut(s) 59
SetI ASST 6 cut(s) 59, 89, 129, 148, 166, 243
SfaNI GCATC 2 cut(s) 126, 170
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 1 cut(s) 218
SsiI CCGC 3 cut(s) 8, 228, 262
SstI GAGCTC 1 cut(s) 59
TaqI TCGA 1 cut(s) 170
TasI AATT 1 cut(s) 218
Tru1I TTAA 3 cut(s) 258, 269, 282
Tru9I TTAA 3 cut(s) 258, 269, 282
TscAI CASTG 2 cut(s) 42, 123
TseI GCWGC 1 cut(s) 63
TspDTI ATGAA 2 cut(s) 196, 242
TspRI CASTG 2 cut(s) 42, 123
Vha464I CTTAAG 1 cut(s) 281
XapI RAATTY 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.