MD13G1172100.v1.1

Monothiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003089
Physical Location & Seq
Reverse (-)
14090464 .. 14090812
349 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1172100.v1.1.491

Sequence Viewer

Length: 309 bp
ATGGAGGCGGTAACAAAGATGGCGTCCGAGAGACCAGTGGTAATCTTCAGCAAGAGCTCGTGCTGCATGTCTCACTCGATCAAAACCCTGCTTTGTGACTTTGGGGTGAACCCAGCAGTGTATGAGCTTGATGAGATGCAAAGAGGGAGAGAGATAGAGCAAGCTCTCTCTAGGCTGGGATGCAACCCTACTGTGCCTGCTGTGTTCATTGGCGGTGAACTTGTGGGTGGAGCCAATGAGGTCATGAGTCTTCATCTTAAGCGCTCCTTAATCCCCATGCTTAAGCGCGTCGGAGCATTATGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

103

Amino Acids

11.15

Weight (kDa)

7.69

Isoelectric Point (pI)

56.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 13 - 75 1.4e-15 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 288
AciI CCGC 2 cut(s) 8, 213
AcuI CTGAAG 1 cut(s) 31
AcyI GRCGYC 1 cut(s) 23
AfeI AGCGCT 1 cut(s) 263
AflII CTTAAG 2 cut(s) 257, 281
AluBI AGCT 3 cut(s) 57, 127, 164
AluI AGCT 3 cut(s) 57, 127, 164
Alw21I GWGCWC 1 cut(s) 59
Alw26I GTCTC 2 cut(s) 25, 75
Aor51HI AGCGCT 1 cut(s) 263
ApeKI GCWGC 1 cut(s) 63
ArsI GACNNNNNNTTYG 2 cut(s) 8, 40
AspLEI GCGC 2 cut(s) 264, 288
AsuHPI GGTGA 2 cut(s) 118, 227
BanII GRGCYC 1 cut(s) 59
BauI CACGAG 1 cut(s) 58
BbsI GAAGAC 1 cut(s) 242
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 1 cut(s) 50
BccI CCATC 1 cut(s) 13
BcoDI GTCTC 2 cut(s) 25, 75
BfaI CTAG 1 cut(s) 171
BfoI RGCGCY 1 cut(s) 265
BfrI CTTAAG 2 cut(s) 257, 281
BisI GCNGC 1 cut(s) 64
BlsI GCNGC 1 cut(s) 65
BmiI GGNNCC 1 cut(s) 232
BmsI GCATC 2 cut(s) 126, 170
BpiI GAAGAC 1 cut(s) 242
BsaHI GRCGYC 1 cut(s) 23
BsaI GGTCTC 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 35
BseGI GGATG 1 cut(s) 185
BseNI ACTGG 1 cut(s) 35
BseXI GCAGC 1 cut(s) 50
BseYI CCCAGC 2 cut(s) 112, 175
Bsh1236I CGCG 1 cut(s) 288
BsiHKAI GWGCWC 1 cut(s) 59
BsmAI GTCTC 2 cut(s) 25, 75
Bso31I GGTCTC 1 cut(s) 25
Bsp1286I GDGCHC 1 cut(s) 59
Bsp143I GATC 1 cut(s) 78
BspACI CCGC 2 cut(s) 8, 213
BspFNI CGCG 1 cut(s) 288
BspHI TCATGA 1 cut(s) 243
BspLI GGNNCC 1 cut(s) 232
BspTI CTTAAG 2 cut(s) 257, 281
BspTNI GGTCTC 1 cut(s) 25
BsrI ACTGG 1 cut(s) 35
BssMI GATC 1 cut(s) 78
BssNI GRCGYC 1 cut(s) 23
BssSI CACGAG 1 cut(s) 58
Bst2BI CACGAG 1 cut(s) 58
Bst4CI ACNGT 1 cut(s) 193
BstACI GRCGYC 1 cut(s) 23
BstAFI CTTAAG 2 cut(s) 257, 281
BstC8I GCNNGC 2 cut(s) 162, 198
BstF5I GGATG 1 cut(s) 185
BstFNI CGCG 1 cut(s) 288
BstH2I RGCGCY 1 cut(s) 265
BstHHI GCGC 2 cut(s) 264, 288
BstKTI GATC 1 cut(s) 81
BstMAI GTCTC 2 cut(s) 25, 75
BstMBI GATC 1 cut(s) 78
BstMWI GCNNNNNNNGC 1 cut(s) 63
BstNSI RCATGY 1 cut(s) 70
BstUI CGCG 1 cut(s) 288
BstV1I GCAGC 1 cut(s) 50
BstV2I GAAGAC 1 cut(s) 242
BtsCI GGATG 1 cut(s) 185
BtsI GCAGTG 1 cut(s) 123
BtsIMutI CAGTG 2 cut(s) 42, 123
Cac8I GCNNGC 2 cut(s) 162, 198
CciI TCATGA 1 cut(s) 243
CfoI GCGC 2 cut(s) 264, 288
CseI GACGC 2 cut(s) 12, 277
CviAII CATG 3 cut(s) 67, 244, 277
CviJI RGCY 5 cut(s) 57, 127, 164, 175, 233
CviKI_1 RGCY 5 cut(s) 57, 127, 164, 175, 233
DpnI GATC 1 cut(s) 80
DpnII GATC 1 cut(s) 78
Ecl136II GAGCTC 1 cut(s) 57
Eco24I GRGCYC 1 cut(s) 59
Eco31I GGTCTC 1 cut(s) 25
Eco47III AGCGCT 1 cut(s) 263
Eco53kI GAGCTC 1 cut(s) 57
Eco57I CTGAAG 1 cut(s) 31
EcoICRI GAGCTC 1 cut(s) 57
EcoT38I GRGCYC 1 cut(s) 59
FaeI CATG 3 cut(s) 70, 247, 280
FaiI YATR 5 cut(s) 68, 123, 245, 278, 301
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FatI CATG 3 cut(s) 66, 243, 276
Fnu4HI GCNGC 1 cut(s) 64
FokI GGATG 1 cut(s) 192
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 1 cut(s) 64
FspBI CTAG 1 cut(s) 171
GlaI GCGC 2 cut(s) 263, 287
GluI GCNGC 1 cut(s) 64
GsaI CCCAGC 2 cut(s) 116, 179
HaeII RGCGCY 1 cut(s) 265
HgaI GACGC 2 cut(s) 12, 277
HhaI GCGC 2 cut(s) 264, 288
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 3 cut(s) 70, 247, 280
Hin6I GCGC 2 cut(s) 262, 286
HinP1I GCGC 2 cut(s) 262, 286
HinfI GANTC 1 cut(s) 247
HphI GGTGA 2 cut(s) 118, 227
Hpy166II GTNNAC 2 cut(s) 109, 218
Hpy188I TCNGA 2 cut(s) 28, 293
Hpy188III TCNNGA 1 cut(s) 244
Hpy8I GTNNAC 2 cut(s) 109, 218
Hpy99I CGWCG 1 cut(s) 293
HpyCH4III ACNGT 1 cut(s) 193
HpyCH4V TGCA 3 cut(s) 66, 139, 183
HpyF10VI GCNNNNNNNGC 1 cut(s) 63
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 3 cut(s) 70, 247, 280
HspAI GCGC 2 cut(s) 262, 286
Kzo9I GATC 1 cut(s) 78
LmnI GCTCC 3 cut(s) 230, 269, 293
LpnPI CCDG 5 cut(s) 48, 101, 126, 161, 210
Lsp1109I GCAGC 1 cut(s) 50
LweI GCATC 2 cut(s) 126, 170
MaeI CTAG 1 cut(s) 171
MaeIII GTNAC 2 cut(s) 10, 95
MalI GATC 1 cut(s) 80
MboI GATC 1 cut(s) 78
MboII GAAGA 2 cut(s) 37, 242
MhlI GDGCHC 1 cut(s) 59
MlyI GAGTC 1 cut(s) 256
MmeI TCCRAC 1 cut(s) 271
MnlI CCTC 2 cut(s) 137, 232
MseI TTAA 3 cut(s) 258, 269, 282
MspCI CTTAAG 2 cut(s) 257, 281
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 1 cut(s) 63
NdeII GATC 1 cut(s) 78
NlaIII CATG 3 cut(s) 70, 247, 280
NlaIV GGNNCC 1 cut(s) 232
NmuCI GTSAC 1 cut(s) 95
NspI RCATGY 1 cut(s) 70
PagI TCATGA 1 cut(s) 243
PkrI GCNGC 1 cut(s) 65
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
Psp124BI GAGCTC 1 cut(s) 59
PspFI CCCAGC 2 cut(s) 112, 175
PspN4I GGNNCC 1 cut(s) 232
SacI GAGCTC 1 cut(s) 59
SaqAI TTAA 3 cut(s) 258, 269, 282
SatI GCNGC 1 cut(s) 64
Sau3AI GATC 1 cut(s) 78
SchI GAGTC 1 cut(s) 256
SduI GDGCHC 1 cut(s) 59
SetI ASST 4 cut(s) 59, 129, 166, 243
SfaNI GCATC 2 cut(s) 126, 170
SmlI CTYRAG 2 cut(s) 257, 281
SmoI CTYRAG 2 cut(s) 257, 281
SsiI CCGC 2 cut(s) 8, 213
SspMI CTAG 1 cut(s) 171
SstI GAGCTC 1 cut(s) 59
TaaI ACNGT 1 cut(s) 193
TaqI TCGA 1 cut(s) 77
Tru1I TTAA 3 cut(s) 258, 269, 282
Tru9I TTAA 3 cut(s) 258, 269, 282
TscAI CASTG 2 cut(s) 42, 123
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 1 cut(s) 63
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 2 cut(s) 196, 242
TspRI CASTG 2 cut(s) 42, 123
Vha464I CTTAAG 2 cut(s) 257, 281
XceI RCATGY 1 cut(s) 70
XspI CTAG 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.