Rorug04G0170700

Monothiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003089
Physical Location & Seq
Reverse (-)
30087357 .. 30093700
6344 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0170700.1

Sequence Viewer

Length: 927 bp
ATGGCGGGCCTCGTTCCTCCTCCACCCACTGCTGCTCCTGATACTCCCAAACAGAATGAGAGAGTCGATTACCTCAATCTCCCTTGCCCTATTCCCTACGACGAGATCTCCCGCGAAGCTTACATGTCTTTAAAGCCAGAGCTTTTCGAGGGGATGCGAATCGACTTTACCAAAGGTCTCAATCAGAAGTTCTCACTCAGTCACAGTGTGACCATGGGACCTACAGAGGTTCCTTCCCAGTCTACTGAAACTATTAAAATTCCCACTGCTCAGTATGAGTTTGGTGCCAATTTTTTGGACCCAAAGCTGATGCTTGTCGGGAGAGTATTGACTGATGGGAGGCTCAATGCGAGAGTAAAGTGTGATTTAACCGAGAATCTTTCTTTGAAGGCTAATTCTCAGCTTACCAACGAGCCACACATGTCACATGGCATGGCCAGTTTTGATTACAAGGGTAAGGATTTCCGGTCACAGTTTCAACTAGGGAATGGTGCATTGTTTGGTGCAAACTATATTCAGAGTGTAACCCCCAATCTGTCCGTGGGTGGTGAAGTGTTTTGGGCTGGTCAGCATCGGAAGTCTGGTATTGGCTATGCTGCTCGATACAATACAGACAAGATGGTTGCCACAGGGCAGGTTGCTAGTACTGGAGTTGTTGCTATGAGCTATGTTCAGAAGGTGTCTGATAAGGTTTCTCTAGCATCAGATTTCATGTACAACTACTTATCAAGAGACGTGACAGCTAGCTTCGGTTATGATTACATTCTGCGACAGTGTCGTCTTAGAGGAAAGATTGATTCCAATGGTGTAGCTGCTGCCTTTTTGGAAGAGCGACTAAATATGGGTCTTAATTTTATTCTTTCTGCAGAGATTGATCATAGAAAGAAAGACTACAAATTTGGGTTTGGATTGACTGTTGGAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

308

Amino Acids

34.06

Weight (kDa)

7.68

Isoelectric Point (pI)

27.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Porin_3 PF01459 29 - 301 3.8e-65 Eukaryotic porin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 777
Acc36I ACCTGC 1 cut(s) 625
AccB1I GGYRCC 1 cut(s) 284
AccI GTMKAC 1 cut(s) 242
AccII CGCG 1 cut(s) 114
AciI CCGC 2 cut(s) 5, 112
AcoI YGGCCR 1 cut(s) 435
AcsI RAATTY 2 cut(s) 258, 896
AdeI CACNNNGTG 1 cut(s) 208
AfaI GTAC 2 cut(s) 646, 716
AflIII ACRYGT 2 cut(s) 123, 420
AgsI TTSAA 2 cut(s) 388, 479
AjiI CACGTC 1 cut(s) 736
AluBI AGCT 8 cut(s) 119, 142, 307, 403, 666, 743, 747, 812
AluI AGCT 8 cut(s) 119, 142, 307, 403, 666, 743, 747, 812
Alw26I GTCTC 2 cut(s) 182, 726
AoxI GGCC 2 cut(s) 7, 435
ApeKI GCWGC 4 cut(s) 32, 596, 812, 815
ApoI RAATTY 2 cut(s) 258, 896
ArsI GACNNNNNNTTYG 2 cut(s) 881, 913
AspS9I GGNCC 3 cut(s) 7, 218, 298
AsuHPI GGTGA 1 cut(s) 560
AsuNHI GCTAGC 1 cut(s) 743
AvaII GGWCC 2 cut(s) 218, 298
BalI TGGCCA 1 cut(s) 437
BanI GGYRCC 1 cut(s) 284
BbvI GCAGC 4 cut(s) 19, 583, 799, 802
BccI CCATC 2 cut(s) 329, 613
BclI TGATCA 1 cut(s) 874
BcoDI GTCTC 2 cut(s) 182, 726
BfaI CTAG 4 cut(s) 482, 642, 698, 744
BfmI CTRYAG 2 cut(s) 222, 864
BfuAI ACCTGC 1 cut(s) 625
BglII AGATCT 1 cut(s) 105
BisI GCNGC 4 cut(s) 33, 597, 813, 816
BlsI GCNGC 4 cut(s) 34, 598, 814, 817
BmcAI AGTACT 1 cut(s) 646
Bme18I GGWCC 2 cut(s) 218, 298
BmgBI CACGTC 1 cut(s) 736
BmgT120I GGNCC 3 cut(s) 7, 218, 298
BmiI GGNNCC 4 cut(s) 219, 231, 286, 300
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 4 cut(s) 144, 300, 580, 710
BmtI GCTAGC 1 cut(s) 747
BmuI ACTGGG 1 cut(s) 232
BpmI CTGGAG 1 cut(s) 669
BsaBI GATNNNNATC 1 cut(s) 158
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 2 cut(s) 213, 540
BsaWI WCCGGW 1 cut(s) 465
BsaXI ACNNNNNCTCC 4 cut(s) 19, 49, 92, 122
Bse1I ACTGG 3 cut(s) 238, 438, 652
Bse8I GATNNNNATC 1 cut(s) 158
BseDI CCNNGG 2 cut(s) 213, 540
BseGI GGATG 1 cut(s) 159
BseJI GATNNNNATC 1 cut(s) 158
BseMII CTCAG 3 cut(s) 211, 284, 413
BseNI ACTGG 3 cut(s) 238, 438, 652
BseRI GAGGAG 1 cut(s) 9
BseXI GCAGC 4 cut(s) 19, 583, 799, 802
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 2 cut(s) 9, 437
BshNI GGYRCC 1 cut(s) 284
BsiSI CCGG 1 cut(s) 466
BslFI GGGAC 1 cut(s) 231
BsmAI GTCTC 2 cut(s) 182, 726
BsmBI CGTCTC 1 cut(s) 726
BsmFI GGGAC 1 cut(s) 231
BsnI GGCC 2 cut(s) 9, 437
Bso31I GGTCTC 1 cut(s) 182
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 2 cut(s) 105, 874
Bsp19I CCATGG 1 cut(s) 213
BspACI CCGC 2 cut(s) 5, 112
BspANI GGCC 2 cut(s) 9, 437
BspCNI CTCAG 3 cut(s) 210, 283, 412
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 4 cut(s) 219, 231, 286, 300
BspMAI CTGCAG 1 cut(s) 868
BspMI ACCTGC 1 cut(s) 625
BspOI GCTAGC 1 cut(s) 747
BspQI GCTCTTC 1 cut(s) 822
BspT107I GGYRCC 1 cut(s) 284
BspTNI GGTCTC 1 cut(s) 182
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 3 cut(s) 238, 438, 652
BssECI CCNNGG 2 cut(s) 213, 540
BssMI GATC 2 cut(s) 105, 874
BssT1I CCWWGG 1 cut(s) 213
Bst4CI ACNGT 4 cut(s) 206, 474, 774, 916
Bst6I CTCTTC 1 cut(s) 822
BstAUI TGTACA 1 cut(s) 714
BstC8I GCNNGC 2 cut(s) 7, 745
BstDEI CTNAG 4 cut(s) 197, 270, 399, 782
BstDSI CCRYGG 2 cut(s) 213, 540
BstF5I GGATG 1 cut(s) 159
BstFNI CGCG 1 cut(s) 114
BstKTI GATC 2 cut(s) 108, 877
BstMAI GTCTC 2 cut(s) 182, 726
BstMBI GATC 2 cut(s) 105, 874
BstNSI RCATGY 2 cut(s) 127, 424
BstSFI CTRYAG 2 cut(s) 222, 864
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 4 cut(s) 19, 583, 799, 802
BstX2I RGATCY 1 cut(s) 105
BstXI CCANNNNNNTGG 1 cut(s) 295
BstYI RGATCY 1 cut(s) 105
BsuRI GGCC 2 cut(s) 9, 437
BtgI CCRYGG 2 cut(s) 213, 540
BtrI CACGTC 1 cut(s) 736
BtsCI GGATG 1 cut(s) 159
BtsI GCAGTG 2 cut(s) 27, 264
BtsIMutI CAGTG 4 cut(s) 27, 211, 264, 779
BveI ACCTGC 1 cut(s) 625
Cac8I GCNNGC 2 cut(s) 7, 745
Cfr13I GGNCC 3 cut(s) 7, 218, 298
Csp6I GTAC 2 cut(s) 645, 715
CviAII CATG 6 cut(s) 124, 214, 421, 428, 433, 712
CviQI GTAC 2 cut(s) 645, 715
DdeI CTNAG 4 cut(s) 197, 270, 399, 782
DpnI GATC 2 cut(s) 107, 876
DpnII GATC 2 cut(s) 105, 874
DraI TTTAAA 1 cut(s) 132
DraIII CACNNNGTG 1 cut(s) 208
DrdI GACNNNNNNGTC 1 cut(s) 777
DseDI GACNNNNNNGTC 1 cut(s) 777
EaeI YGGCCR 1 cut(s) 435
Eam1104I CTCTTC 1 cut(s) 822
EarI CTCTTC 1 cut(s) 822
Eco130I CCWWGG 1 cut(s) 213
Eco31I GGTCTC 1 cut(s) 182
Eco47I GGWCC 2 cut(s) 218, 298
EcoO109I RGGNCCY 1 cut(s) 218
EcoT14I CCWWGG 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 213
Esp3I CGTCTC 1 cut(s) 726
FaeI CATG 6 cut(s) 127, 217, 424, 431, 436, 715
FaqI GGGAC 1 cut(s) 231
FatI CATG 6 cut(s) 123, 213, 420, 427, 432, 711
FauI CCCGC 1 cut(s) 119
FbaI TGATCA 1 cut(s) 874
FblI GTMKAC 1 cut(s) 242
Fnu4HI GCNGC 4 cut(s) 33, 597, 813, 816
FokI GGATG 1 cut(s) 166
Fsp4HI GCNGC 4 cut(s) 33, 597, 813, 816
FspBI CTAG 4 cut(s) 482, 642, 698, 744
GluI GCNGC 4 cut(s) 33, 597, 813, 816
GsuI CTGGAG 1 cut(s) 669
HaeIII GGCC 2 cut(s) 9, 437
HapII CCGG 1 cut(s) 466
Hin1II CATG 6 cut(s) 127, 217, 424, 431, 436, 715
HindIII AAGCTT 1 cut(s) 117
HinfI GANTC 4 cut(s) 63, 159, 376, 797
HpaII CCGG 1 cut(s) 466
HphI GGTGA 1 cut(s) 560
Hpy166II GTNNAC 1 cut(s) 243
Hpy188I TCNGA 6 cut(s) 186, 519, 576, 675, 685, 706
Hpy188III TCNNGA 3 cut(s) 38, 319, 729
Hpy8I GTNNAC 1 cut(s) 243
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 3 cut(s) 243, 382, 670
HpyCH4III ACNGT 4 cut(s) 206, 474, 774, 916
HpyCH4IV ACGT 1 cut(s) 735
HpyCH4V TGCA 3 cut(s) 494, 506, 866
HpyF3I CTNAG 4 cut(s) 197, 270, 399, 782
HpySE526I ACGT 1 cut(s) 735
Hsp92II CATG 6 cut(s) 127, 217, 424, 431, 436, 715
Ksp22I TGATCA 1 cut(s) 874
Kzo9I GATC 2 cut(s) 105, 874
LguI GCTCTTC 1 cut(s) 822
LmnI GCTCC 1 cut(s) 40
Lsp1109I GCAGC 4 cut(s) 19, 583, 799, 802
LweI GCATC 4 cut(s) 144, 300, 580, 710
MaeI CTAG 4 cut(s) 482, 642, 698, 744
MaeII ACGT 1 cut(s) 735
MaeIII GTNAC 6 cut(s) 200, 208, 423, 468, 523, 736
MalI GATC 2 cut(s) 107, 876
MboI GATC 2 cut(s) 105, 874
MboII GAAGA 1 cut(s) 839
MflI RGATCY 1 cut(s) 105
MlsI TGGCCA 1 cut(s) 437
MluCI AATT 5 cut(s) 258, 289, 394, 850, 896
MluNI TGGCCA 1 cut(s) 437
MlyI GAGTC 1 cut(s) 72
MmeI TCCRAC 1 cut(s) 898
MnlI CCTC 8 cut(s) 20, 27, 30, 83, 142, 220, 333, 779
Mox20I TGGCCA 1 cut(s) 437
MscI TGGCCA 1 cut(s) 437
MseI TTAA 4 cut(s) 131, 255, 368, 849
Msp20I TGGCCA 1 cut(s) 437
MspI CCGG 1 cut(s) 466
MvnI CGCG 1 cut(s) 114
NcoI CCATGG 1 cut(s) 213
NdeII GATC 2 cut(s) 105, 874
NheI GCTAGC 1 cut(s) 743
NlaIII CATG 6 cut(s) 127, 217, 424, 431, 436, 715
NlaIV GGNNCC 4 cut(s) 219, 231, 286, 300
NmuCI GTSAC 5 cut(s) 200, 208, 423, 468, 736
NspI RCATGY 2 cut(s) 127, 424
PciI ACATGT 2 cut(s) 123, 420
PciSI GCTCTTC 1 cut(s) 822
PfeI GAWTC 3 cut(s) 159, 376, 797
PflFI GACNNNGTC 1 cut(s) 774
PkrI GCNGC 4 cut(s) 34, 598, 814, 817
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
PpuMI RGGWCCY 1 cut(s) 218
PscI ACATGT 2 cut(s) 123, 420
Psp5II RGGWCCY 1 cut(s) 218
PspN4I GGNNCC 4 cut(s) 219, 231, 286, 300
PspPI GGNCC 3 cut(s) 7, 218, 298
PspPPI RGGWCCY 1 cut(s) 218
PstI CTGCAG 1 cut(s) 868
PsuI RGATCY 1 cut(s) 105
PsyI GACNNNGTC 1 cut(s) 774
RsaI GTAC 2 cut(s) 646, 716
RsaNI GTAC 2 cut(s) 645, 715
SapI GCTCTTC 1 cut(s) 822
SaqAI TTAA 4 cut(s) 131, 255, 368, 849
SatI GCNGC 4 cut(s) 33, 597, 813, 816
Sau3AI GATC 2 cut(s) 105, 874
Sau96I GGNCC 3 cut(s) 7, 218, 298
ScaI AGTACT 1 cut(s) 646
SchI GAGTC 1 cut(s) 72
SfaNI GCATC 4 cut(s) 144, 300, 580, 710
SfcI CTRYAG 2 cut(s) 222, 864
SinI GGWCC 2 cut(s) 218, 298
Sse9I AATT 5 cut(s) 258, 289, 394, 850, 896
SsiI CCGC 2 cut(s) 5, 112
SspMI CTAG 4 cut(s) 482, 642, 698, 744
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 4 cut(s) 206, 474, 774, 916
TaiI ACGT 1 cut(s) 738
TaqI TCGA 4 cut(s) 66, 147, 162, 601
TasI AATT 5 cut(s) 258, 289, 394, 850, 896
TatI WGTACW 2 cut(s) 644, 714
TfiI GAWTC 3 cut(s) 159, 376, 797
Tru1I TTAA 4 cut(s) 131, 255, 368, 849
Tru9I TTAA 4 cut(s) 131, 255, 368, 849
TscAI CASTG 4 cut(s) 34, 211, 271, 779
TseFI GTSAC 5 cut(s) 200, 208, 423, 468, 736
TseI GCWGC 4 cut(s) 32, 596, 812, 815
Tsp45I GTSAC 5 cut(s) 200, 208, 423, 468, 736
TspDTI ATGAA 1 cut(s) 700
TspGWI ACGGA 1 cut(s) 529
TspRI CASTG 4 cut(s) 34, 211, 271, 779
Tth111I GACNNNGTC 1 cut(s) 774
VpaK11BI GGWCC 2 cut(s) 218, 298
XapI RAATTY 2 cut(s) 258, 896
XceI RCATGY 2 cut(s) 127, 424
XcmI CCANNNNNNNNNTGG 1 cut(s) 538
XmiI GTMKAC 1 cut(s) 242
XspI CTAG 4 cut(s) 482, 642, 698, 744
ZrmI AGTACT 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.