FvH4_4g17501

Monothiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003089
Physical Location & Seq
Forward (+)
21456007 .. 21456852
846 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17501.t1

Sequence Viewer

Length: 324 bp
ATGGAGGTGGTGACAAAGTTGGCTTCCGAGAGACCTGTGGTGATCTTTAGCAAGAGCTCATGCTGCATGTCCTACACCATCAAGACCTTGTTTTGTGATTTTGGGGTCAACCCAGCAGTGCATGAGCTTGATGAGATGCAAAGAGGGAAAGAAATAGAGCAAGCTCTGTTGAGGCTCCGGTGCAGCCCATCTGTTCCGGCTGTGTTCATTGGTGGTGAATTTGTTGGCGGAGCCAATGAAGTCATGAGTCTTCATCTTAAACGCTCCTTAATCCCCATGCTTAAGCGCGTTGGCGCATTATGGTGCATGCATGAGCTGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

108

Amino Acids

12.0

Weight (kDa)

7.66

Isoelectric Point (pI)

68.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 13 - 75 6.5e-13 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 288
AciI CCGC 1 cut(s) 228
AcsI RAATTY 1 cut(s) 218
AflII CTTAAG 1 cut(s) 281
AluBI AGCT 4 cut(s) 57, 127, 164, 316
AluI AGCT 4 cut(s) 57, 127, 164, 316
Alw21I GWGCWC 1 cut(s) 59
Alw26I GTCTC 1 cut(s) 25
ApeKI GCWGC 2 cut(s) 63, 183
ApoI RAATTY 1 cut(s) 218
AspLEI GCGC 2 cut(s) 288, 296
AsuHPI GGTGA 3 cut(s) 22, 52, 227
BanII GRGCYC 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 242
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 2 cut(s) 50, 195
BccI CCATC 2 cut(s) 86, 196
BcoDI GTCTC 1 cut(s) 25
BfrI CTTAAG 1 cut(s) 281
BisI GCNGC 2 cut(s) 64, 184
BlsI GCNGC 2 cut(s) 65, 185
BmiI GGNNCC 2 cut(s) 176, 232
BmsI GCATC 1 cut(s) 126
BpiI GAAGAC 1 cut(s) 242
BsaI GGTCTC 1 cut(s) 25
BsaWI WCCGGW 1 cut(s) 177
BseXI GCAGC 2 cut(s) 50, 195
BseYI CCCAGC 1 cut(s) 112
BsgI GTGCAG 1 cut(s) 202
Bsh1236I CGCG 1 cut(s) 288
BsiHKAI GWGCWC 1 cut(s) 59
BsiSI CCGG 2 cut(s) 178, 197
BsmAI GTCTC 1 cut(s) 25
Bso31I GGTCTC 1 cut(s) 25
Bsp1286I GDGCHC 1 cut(s) 59
Bsp143I GATC 1 cut(s) 42
BspACI CCGC 1 cut(s) 228
BspFNI CGCG 1 cut(s) 288
BspHI TCATGA 1 cut(s) 243
BspLI GGNNCC 2 cut(s) 176, 232
BspTI CTTAAG 1 cut(s) 281
BspTNI GGTCTC 1 cut(s) 25
BssMI GATC 1 cut(s) 42
BstAFI CTTAAG 1 cut(s) 281
BstC8I GCNNGC 2 cut(s) 162, 308
BstFNI CGCG 1 cut(s) 288
BstHHI GCGC 2 cut(s) 288, 296
BstKTI GATC 1 cut(s) 45
BstMAI GTCTC 1 cut(s) 25
BstMBI GATC 1 cut(s) 42
BstMWI GCNNNNNNNGC 1 cut(s) 63
BstNSI RCATGY 2 cut(s) 70, 310
BstUI CGCG 1 cut(s) 288
BstV1I GCAGC 2 cut(s) 50, 195
BstV2I GAAGAC 1 cut(s) 242
BtsI GCAGTG 1 cut(s) 123
BtsIMutI CAGTG 1 cut(s) 123
Cac8I GCNNGC 2 cut(s) 162, 308
CciI TCATGA 1 cut(s) 243
CfoI GCGC 2 cut(s) 288, 296
CviAII CATG 7 cut(s) 60, 67, 122, 244, 277, 307, 311
CviJI RGCY 9 cut(s) 23, 57, 127, 164, 175, 186, 200, 233, 316
CviKI_1 RGCY 9 cut(s) 23, 57, 127, 164, 175, 186, 200, 233, 316
DpnI GATC 1 cut(s) 44
DpnII GATC 1 cut(s) 42
EciI GGCGGA 1 cut(s) 243
Ecl136II GAGCTC 1 cut(s) 57
Eco24I GRGCYC 1 cut(s) 59
Eco31I GGTCTC 1 cut(s) 25
Eco53kI GAGCTC 1 cut(s) 57
EcoICRI GAGCTC 1 cut(s) 57
EcoT22I ATGCAT 1 cut(s) 312
EcoT38I GRGCYC 1 cut(s) 59
FaeI CATG 7 cut(s) 63, 70, 125, 247, 280, 310, 314
FaiI YATR 8 cut(s) 61, 68, 123, 245, 278, 301, 308, 312
FatI CATG 7 cut(s) 59, 66, 121, 243, 276, 306, 310
Fnu4HI GCNGC 2 cut(s) 64, 184
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 2 cut(s) 64, 184
GlaI GCGC 2 cut(s) 287, 295
GluI GCNGC 2 cut(s) 64, 184
GsaI CCCAGC 1 cut(s) 116
HapII CCGG 2 cut(s) 178, 197
HhaI GCGC 2 cut(s) 288, 296
Hin1II CATG 7 cut(s) 63, 70, 125, 247, 280, 310, 314
Hin6I GCGC 2 cut(s) 286, 294
HinP1I GCGC 2 cut(s) 286, 294
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HinfI GANTC 1 cut(s) 247
HpaII CCGG 2 cut(s) 178, 197
HphI GGTGA 3 cut(s) 22, 52, 227
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 1 cut(s) 28
Hpy188III TCNNGA 2 cut(s) 82, 244
Hpy8I GTNNAC 1 cut(s) 109
HpyCH4V TGCA 6 cut(s) 66, 121, 139, 183, 306, 310
HpyF10VI GCNNNNNNNGC 1 cut(s) 63
Hsp92II CATG 7 cut(s) 63, 70, 125, 247, 280, 310, 314
HspAI GCGC 2 cut(s) 286, 294
Kzo9I GATC 1 cut(s) 42
LmnI GCTCC 3 cut(s) 180, 230, 269
LpnPI CCDG 4 cut(s) 48, 126, 191, 210
Lsp1109I GCAGC 2 cut(s) 50, 195
LweI GCATC 1 cut(s) 126
MaeIII GTNAC 1 cut(s) 10
MalI GATC 1 cut(s) 44
MboI GATC 1 cut(s) 42
MboII GAAGA 1 cut(s) 242
MhlI GDGCHC 1 cut(s) 59
MluCI AATT 1 cut(s) 218
MlyI GAGTC 1 cut(s) 256
MnlI CCTC 2 cut(s) 137, 165
Mph1103I ATGCAT 1 cut(s) 312
MseI TTAA 3 cut(s) 258, 269, 282
MslI CAYNNNNRTG 1 cut(s) 301
MspCI CTTAAG 1 cut(s) 281
MspI CCGG 2 cut(s) 178, 197
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 1 cut(s) 63
NdeII GATC 1 cut(s) 42
NlaIII CATG 7 cut(s) 63, 70, 125, 247, 280, 310, 314
NlaIV GGNNCC 2 cut(s) 176, 232
NmuCI GTSAC 1 cut(s) 10
NsiI ATGCAT 1 cut(s) 312
NspI RCATGY 2 cut(s) 70, 310
PaeI GCATGC 1 cut(s) 310
PagI TCATGA 1 cut(s) 243
PkrI GCNGC 2 cut(s) 65, 185
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
Psp124BI GAGCTC 1 cut(s) 59
PspFI CCCAGC 1 cut(s) 112
PspN4I GGNNCC 2 cut(s) 176, 232
RseI CAYNNNNRTG 1 cut(s) 301
SacI GAGCTC 1 cut(s) 59
SaqAI TTAA 3 cut(s) 258, 269, 282
SatI GCNGC 2 cut(s) 64, 184
Sau3AI GATC 1 cut(s) 42
SchI GAGTC 1 cut(s) 256
SduI GDGCHC 1 cut(s) 59
SetI ASST 7 cut(s) 9, 37, 59, 89, 129, 166, 318
SfaNI GCATC 1 cut(s) 126
SmiMI CAYNNNNRTG 1 cut(s) 301
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
SphI GCATGC 1 cut(s) 310
Sse9I AATT 1 cut(s) 218
SsiI CCGC 1 cut(s) 228
SstI GAGCTC 1 cut(s) 59
TasI AATT 1 cut(s) 218
Tru1I TTAA 3 cut(s) 258, 269, 282
Tru9I TTAA 3 cut(s) 258, 269, 282
TscAI CASTG 1 cut(s) 123
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 2 cut(s) 63, 183
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 3 cut(s) 196, 242, 252
TspRI CASTG 1 cut(s) 123
Vha464I CTTAAG 1 cut(s) 281
XapI RAATTY 1 cut(s) 218
XceI RCATGY 2 cut(s) 70, 310
Zsp2I ATGCAT 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.