FvH4_4g19220

DNA polymerase III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003855
Physical Location & Seq
Reverse (-)
22919609 .. 22920673
1065 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19220.t1

Sequence Viewer

Length: 783 bp
ATGATGAGAACAAGCTCCTTGTGCTTTTCTAACTTCCAATTACCTAAGTTTTGCCTTCAGTCACCATCTATTGGATTAGCATCTTTAGGAAGAACTTGGGCACGAAACTTTAAGGTACTTAGAACTAGTAGTCCTACTGAATCATCTGAAAACTACTTCAAAAGCATACAATTGTCTGATGCTCCAGCTAAGATAATTTGGGACGAAAAAACTGTAAAGCCGTCTCCTGAGACTATGTTGGTTTTTGATCTTGAAACAACTGAACTTGACACTAAGAAAGGAGGAATCGTTGAAGTCGGAATGAAGGATGTCCAAGGTGGCAAGAACAGTTGTTTCCAGACACTAGTAAACCCTGAACAAGATATTCTAAATTCTTACATCCATGGTATAACAACCGACATGGTGAGACATACAGATGTTCCAACGATGAAAGAAGCGATTCCATTCCTTCTCCGGTTCATCCAAAGCCGCCAAGTGCTTGGAGGGAAAGTTTTGCTTGCTGCTCACAATGCAAAACGTTTCGATGCACCCTTCTTGATTGAAGCATTCAGACGCTGCTCATTTGATTTTCCTAATGACTTGCTGTTTCTCGACACCCTCGCTCTGGCACGAGAAGTGAAGAAACTGATCCCTTTAGAGGGCCAACCTCATAGGGCCATGACAGATGTAAACTTGCTAACATCTGTACTGGATAACATGATTTCTGATCTGAAGCTTGGTCCGGCTGACCTATTGCAAAGAGCTTTCAAGGCTTCGGATTTGAATATATCAAAGAAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T

Protein Analysis

261

Amino Acids

29.11

Weight (kDa)

8.62

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 80 - 222 1e-13 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 71
AciI CCGC 1 cut(s) 469
AclI AACGTT 1 cut(s) 517
AclWI GGATC 1 cut(s) 622
AcsI RAATTY 1 cut(s) 370
AcuI CTGAAG 2 cut(s) 41, 731
AfaI GTAC 2 cut(s) 117, 687
AfiI CCNNNNNNNGG 4 cut(s) 71, 604, 637, 638
AgsI TTSAA 6 cut(s) 160, 254, 293, 542, 748, 763
AhlI ACTAGT 2 cut(s) 125, 343
AloI GAACNNNNNNTCC 2 cut(s) 115, 147
AluBI AGCT 5 cut(s) 15, 188, 715, 743, 780
AluI AGCT 5 cut(s) 15, 188, 715, 743, 780
Alw26I GTCTC 3 cut(s) 224, 228, 400
AlwI GGATC 1 cut(s) 622
AlwNI CAGNNNCTG 1 cut(s) 555
AoxI GGCC 2 cut(s) 640, 654
ApeKI GCWGC 2 cut(s) 500, 555
ApoI RAATTY 1 cut(s) 370
Asp700I GAANNNNTTC 1 cut(s) 438
AspS9I GGNCC 3 cut(s) 640, 654, 719
AsuHPI GGTGA 2 cut(s) 54, 415
AvaII GGWCC 1 cut(s) 719
BaeGI GKGCMC 1 cut(s) 103
BauI CACGAG 1 cut(s) 609
BbvI GCAGC 2 cut(s) 487, 542
BccI CCATC 1 cut(s) 73
BceAI ACGGC 1 cut(s) 205
BcoDI GTCTC 3 cut(s) 224, 228, 400
BcuI ACTAGT 2 cut(s) 125, 343
BfaI CTAG 2 cut(s) 126, 344
BisI GCNGC 3 cut(s) 469, 501, 556
BlsI GCNGC 3 cut(s) 470, 502, 557
Bme18I GGWCC 1 cut(s) 719
BmgT120I GGNCC 3 cut(s) 640, 654, 719
BmsI GCATC 3 cut(s) 89, 169, 514
BpmI CTGGAG 1 cut(s) 168
BsaBI GATNNNNATC 1 cut(s) 79
BsaJI CCNNGG 2 cut(s) 313, 382
BsaWI WCCGGW 1 cut(s) 453
BsaXI ACNNNNNCTCC 4 cut(s) 273, 303, 474, 504
Bsc4I CCNNNNNNNGG 4 cut(s) 71, 604, 637, 638
Bse1I ACTGG 1 cut(s) 693
Bse8I GATNNNNATC 1 cut(s) 79
BseDI CCNNGG 2 cut(s) 313, 382
BseGI GGATG 3 cut(s) 313, 378, 459
BseJI GATNNNNATC 1 cut(s) 79
BseLI CCNNNNNNNGG 4 cut(s) 71, 604, 637, 638
BseMII CTCAG 1 cut(s) 219
BseNI ACTGG 1 cut(s) 693
BseSI GKGCMC 1 cut(s) 103
BseXI GCAGC 2 cut(s) 487, 542
BshFI GGCC 2 cut(s) 642, 656
BsiSI CCGG 2 cut(s) 454, 722
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 4 cut(s) 71, 604, 637, 638
BsmAI GTCTC 3 cut(s) 224, 228, 400
BsmBI CGTCTC 1 cut(s) 228
BsmFI GGGAC 1 cut(s) 215
BsmI GAATGC 1 cut(s) 545
BsnI GGCC 2 cut(s) 642, 656
Bsp1286I GDGCHC 1 cut(s) 103
Bsp143I GATC 3 cut(s) 247, 627, 706
Bsp19I CCATGG 1 cut(s) 382
BspACI CCGC 1 cut(s) 469
BspANI GGCC 2 cut(s) 642, 656
BspCNI CTCAG 1 cut(s) 220
BspPI GGATC 1 cut(s) 622
BspQI GCTCTTC 1 cut(s) 770
BsrI ACTGG 1 cut(s) 693
BssECI CCNNGG 2 cut(s) 313, 382
BssMI GATC 3 cut(s) 247, 627, 706
BssSI CACGAG 1 cut(s) 609
BssT1I CCWWGG 2 cut(s) 313, 382
Bst2BI CACGAG 1 cut(s) 609
Bst4CI ACNGT 2 cut(s) 214, 329
Bst6I CTCTTC 1 cut(s) 770
BstC8I GCNNGC 1 cut(s) 498
BstDEI CTNAG 5 cut(s) 45, 119, 189, 228, 273
BstDSI CCRYGG 1 cut(s) 382
BstF5I GGATG 3 cut(s) 313, 378, 459
BstKTI GATC 3 cut(s) 250, 630, 709
BstMAI GTCTC 3 cut(s) 224, 228, 400
BstMBI GATC 3 cut(s) 247, 627, 706
BstMWI GCNNNNNNNGC 3 cut(s) 21, 509, 749
BstSLI GKGCMC 1 cut(s) 103
BstV1I GCAGC 2 cut(s) 487, 542
BstXI CCANNNNNNTGG 1 cut(s) 479
BsuRI GGCC 2 cut(s) 642, 656
BtgI CCRYGG 1 cut(s) 382
BtsCI GGATG 3 cut(s) 313, 378, 459
Cac8I GCNNGC 1 cut(s) 498
CaiI CAGNNNCTG 1 cut(s) 555
Cfr13I GGNCC 3 cut(s) 640, 654, 719
CseI GACGC 1 cut(s) 561
Csp6I GTAC 2 cut(s) 116, 686
CviAII CATG 4 cut(s) 383, 400, 658, 697
CviQI GTAC 2 cut(s) 116, 686
DdeI CTNAG 5 cut(s) 45, 119, 189, 228, 273
DpnI GATC 3 cut(s) 249, 629, 708
DpnII GATC 3 cut(s) 247, 627, 706
Eam1104I CTCTTC 1 cut(s) 770
EarI CTCTTC 1 cut(s) 770
Eco130I CCWWGG 2 cut(s) 313, 382
Eco47I GGWCC 1 cut(s) 719
Eco57I CTGAAG 2 cut(s) 41, 731
EcoT14I CCWWGG 2 cut(s) 313, 382
ErhI CCWWGG 2 cut(s) 313, 382
Esp3I CGTCTC 1 cut(s) 228
FaeI CATG 4 cut(s) 386, 403, 661, 700
FalI AAGNNNNNCTT 2 cut(s) 480, 512
FaqI GGGAC 1 cut(s) 215
FatI CATG 4 cut(s) 382, 399, 657, 696
Fnu4HI GCNGC 3 cut(s) 469, 501, 556
FokI GGATG 3 cut(s) 320, 365, 446
Fsp4HI GCNGC 3 cut(s) 469, 501, 556
FspBI CTAG 2 cut(s) 126, 344
GluI GCNGC 3 cut(s) 469, 501, 556
GsuI CTGGAG 1 cut(s) 168
HaeIII GGCC 2 cut(s) 642, 656
HapII CCGG 2 cut(s) 454, 722
HgaI GACGC 1 cut(s) 561
Hin1II CATG 4 cut(s) 386, 403, 661, 700
HindIII AAGCTT 1 cut(s) 713
HinfI GANTC 3 cut(s) 140, 285, 439
HpaII CCGG 2 cut(s) 454, 722
HphI GGTGA 2 cut(s) 54, 415
Hpy166II GTNNAC 2 cut(s) 349, 670
Hpy188I TCNGA 7 cut(s) 148, 178, 299, 551, 706, 711, 757
Hpy188III TCNNGA 5 cut(s) 227, 251, 337, 535, 590
Hpy8I GTNNAC 2 cut(s) 349, 670
HpyAV CCTTC 4 cut(s) 65, 298, 458, 541
HpyCH4III ACNGT 2 cut(s) 214, 329
HpyCH4IV ACGT 1 cut(s) 517
HpyCH4V TGCA 3 cut(s) 512, 527, 736
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 509, 749
HpyF3I CTNAG 5 cut(s) 45, 119, 189, 228, 273
HpySE526I ACGT 1 cut(s) 517
Hsp92II CATG 4 cut(s) 386, 403, 661, 700
Kzo9I GATC 3 cut(s) 247, 627, 706
LguI GCTCTTC 1 cut(s) 770
LmnI GCTCC 2 cut(s) 20, 187
LpnPI CCDG 8 cut(s) 198, 240, 350, 366, 467, 590, 674, 735
Lsp1109I GCAGC 2 cut(s) 487, 542
LweI GCATC 3 cut(s) 89, 169, 514
MaeI CTAG 2 cut(s) 126, 344
MaeII ACGT 1 cut(s) 517
MaeIII GTNAC 1 cut(s) 60
MalI GATC 3 cut(s) 249, 629, 708
MboI GATC 3 cut(s) 247, 627, 706
MboII GAAGA 2 cut(s) 102, 631
MfeI CAATTG 1 cut(s) 170
MhlI GDGCHC 1 cut(s) 103
MluCI AATT 4 cut(s) 38, 170, 195, 370
MmeI TCCRAC 2 cut(s) 277, 446
MnlI CCTC 5 cut(s) 275, 476, 608, 631, 657
MroXI GAANNNNTTC 1 cut(s) 438
MseI TTAA 1 cut(s) 111
MslI CAYNNNNRTG 1 cut(s) 414
MspI CCGG 2 cut(s) 454, 722
MunI CAATTG 1 cut(s) 170
Mva1269I GAATGC 1 cut(s) 545
MwoI GCNNNNNNNGC 3 cut(s) 21, 509, 749
NcoI CCATGG 1 cut(s) 382
NdeII GATC 3 cut(s) 247, 627, 706
NlaIII CATG 4 cut(s) 386, 403, 661, 700
NmuCI GTSAC 1 cut(s) 60
PciSI GCTCTTC 1 cut(s) 770
PctI GAATGC 1 cut(s) 545
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 3 cut(s) 140, 285, 439
PflMI CCANNNNNTGG 1 cut(s) 71
PkrI GCNGC 3 cut(s) 470, 502, 557
Psp1406I AACGTT 1 cut(s) 517
PspPI GGNCC 3 cut(s) 640, 654, 719
PstNI CAGNNNCTG 1 cut(s) 555
RsaI GTAC 2 cut(s) 117, 687
RsaNI GTAC 2 cut(s) 116, 686
RseI CAYNNNNRTG 1 cut(s) 414
SapI GCTCTTC 1 cut(s) 770
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 3 cut(s) 469, 501, 556
Sau3AI GATC 3 cut(s) 247, 627, 706
Sau96I GGNCC 3 cut(s) 640, 654, 719
SduI GDGCHC 1 cut(s) 103
SfaNI GCATC 3 cut(s) 89, 169, 514
SinI GGWCC 1 cut(s) 719
SmiMI CAYNNNNRTG 1 cut(s) 414
SpeI ACTAGT 2 cut(s) 125, 343
Sse9I AATT 4 cut(s) 38, 170, 195, 370
SsiI CCGC 1 cut(s) 469
SspMI CTAG 2 cut(s) 126, 344
StyI CCWWGG 2 cut(s) 313, 382
TaaI ACNGT 2 cut(s) 214, 329
TaiI ACGT 1 cut(s) 520
TaqI TCGA 2 cut(s) 522, 591
TasI AATT 4 cut(s) 38, 170, 195, 370
TatI WGTACW 1 cut(s) 685
TauI GCSGC 1 cut(s) 471
TfiI GAWTC 3 cut(s) 140, 285, 439
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TseFI GTSAC 1 cut(s) 60
TseI GCWGC 2 cut(s) 500, 555
Tsp45I GTSAC 1 cut(s) 60
TspDTI ATGAA 3 cut(s) 317, 443, 448
Van91I CCANNNNNTGG 1 cut(s) 71
VpaK11BI GGWCC 1 cut(s) 719
XapI RAATTY 1 cut(s) 370
XmnI GAANNNNTTC 1 cut(s) 438
XspI CTAG 2 cut(s) 126, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.