RLG00000007497

DNA polymerase III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003855
Physical Location & Seq
Forward (+)
17583927 .. 17585028
1102 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007497

Sequence Viewer

Length: 864 bp
ATGATGAGAACATGCTCCATGTGCTACTCTAACTTCCAAGTACCCAAGTTTTGCAAAACTTTAAGGTACTTAGTACCATCTATTCGATTAGCATCTTTAGGAAGAACTTGGGCACAAAACTTTAAGGTACTTACCACTAGGAGTACTACTGAATCATCTGCAAACTGCTTCAGAAGCATACAATACTCTGATGCTCAAGCTAAGATAATTTTGGACGACAAAATTGTAAAACCGCCTCCTGTTAATGTGTTGGTTTTTGATATTGAAACCACTGGACTTCACCCAAAGAAAGGAGGAATCATTGAAATTGCATTCCGGGATGTTCAAGGTGGCAAGAACAGTTGTTTCCAGACCCTAGTAAACCCCGAGCAAGATGTTCCAAATTCTGATATCCATGGTATAAGAACTGTCATGGTGAGACAGACAGATGTCCCAACGATGAAGGAATTGATTCCATTCCTTCTCCAATACATCCAAAGCCGCCAAGTGTTTGGAGGGCAAGTTTTGCTTGCTGCTCACAATGCAAGACGTTTCGATGTACCCTTTTTGATTGAGGCATTCAGACGCTGCTCATTCGATATTCCTCATGACTTGCTGTTTCTCGACACCCTCCCTCTGGCACGAGAAGTGATGAAACTGTATGGACCAAAGGCTTCTAGAAAGCTCTCACTTCAGGCACTTGGTGAGTTCTATAGGATCCCTTTAGAGGGTTCAGCTCATAGGGCCATGACTGACGTGAACTTGCTAACATCTATACTTGCGAACATGATTTCCGACCTGAAGCTTGGTTCCACTGACCTTTTGCAAAGAACTTTCAAGGCTTCAGATGTGAATAACTCGAAGAAGAACCAGAAGAAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T

Protein Analysis

288

Amino Acids

32.39

Weight (kDa)

9.6

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 84 - 250 6.6e-19 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 233, 481
AclWI GGATC 2 cut(s) 691, 704
AcsI RAATTY 1 cut(s) 382
AcuI CTGAAG 4 cut(s) 154, 656, 800, 807
AdeI CACNNNGTG 1 cut(s) 683
AfaI GTAC 6 cut(s) 42, 68, 75, 129, 145, 540
AfiI CCNNNNNNNGG 4 cut(s) 290, 616, 706, 707
AgsI TTSAA 4 cut(s) 266, 305, 326, 817
AjiI CACGTC 1 cut(s) 736
AloI GAACNNNNNNTCC 2 cut(s) 755, 787
AluBI AGCT 5 cut(s) 200, 664, 716, 784, 861
AluI AGCT 5 cut(s) 200, 664, 716, 784, 861
Alw26I GTCTC 1 cut(s) 412
AlwI GGATC 2 cut(s) 691, 704
AlwNI CAGNNNCTG 1 cut(s) 567
Ama87I CYCGRG 1 cut(s) 365
AoxI GGCC 1 cut(s) 723
ApeKI GCWGC 2 cut(s) 512, 567
ApoI RAATTY 1 cut(s) 382
Asp700I GAANNNNTTC 1 cut(s) 450
AspS9I GGNCC 2 cut(s) 644, 723
AsuC2I CCSGG 1 cut(s) 317
AsuHPI GGTGA 3 cut(s) 272, 427, 695
AvaI CYCGRG 1 cut(s) 365
AvaII GGWCC 1 cut(s) 644
BaeGI GKGCMC 1 cut(s) 115
BamHI GGATCC 1 cut(s) 696
BauI CACGAG 1 cut(s) 621
BbvI GCAGC 2 cut(s) 499, 554
BccI CCATC 1 cut(s) 85
BcnI CCSGG 1 cut(s) 317
BcoDI GTCTC 1 cut(s) 412
BfaI CTAG 3 cut(s) 138, 356, 657
BfmI CTRYAG 1 cut(s) 691
BisI GCNGC 3 cut(s) 481, 513, 568
BlsI GCNGC 3 cut(s) 482, 514, 569
BmcAI AGTACT 1 cut(s) 145
Bme1390I CCNGG 1 cut(s) 317
Bme18I GGWCC 1 cut(s) 644
BmeT110I CYCGRG 1 cut(s) 365
BmgBI CACGTC 1 cut(s) 736
BmgT120I GGNCC 2 cut(s) 644, 723
BmiI GGNNCC 2 cut(s) 698, 790
BmrFI CCNGG 1 cut(s) 317
BmsI GCATC 2 cut(s) 101, 181
BoxI GACNNNNGTC 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 180
BpuMI CCSGG 1 cut(s) 317
BsaBI GATNNNNATC 1 cut(s) 91
BsaJI CCNNGG 1 cut(s) 394
BsaXI ACNNNNNCTCC 2 cut(s) 486, 516
Bsc4I CCNNNNNNNGG 4 cut(s) 290, 616, 706, 707
Bse1I ACTGG 1 cut(s) 277
Bse8I GATNNNNATC 1 cut(s) 91
BseDI CCNNGG 1 cut(s) 394
BseGI GGATG 2 cut(s) 325, 471
BseJI GATNNNNATC 1 cut(s) 91
BseLI CCNNNNNNNGG 4 cut(s) 290, 616, 706, 707
BseNI ACTGG 1 cut(s) 277
BseSI GKGCMC 1 cut(s) 115
BseXI GCAGC 2 cut(s) 499, 554
BshFI GGCC 1 cut(s) 725
BsiHKCI CYCGRG 1 cut(s) 365
BsiSI CCGG 1 cut(s) 316
BslFI GGGAC 1 cut(s) 416
BslI CCNNNNNNNGG 4 cut(s) 290, 616, 706, 707
BsmAI GTCTC 1 cut(s) 412
BsmFI GGGAC 1 cut(s) 416
BsmI GAATGC 2 cut(s) 311, 557
BsnI GGCC 1 cut(s) 725
BsoBI CYCGRG 1 cut(s) 365
Bsp1286I GDGCHC 1 cut(s) 115
Bsp143I GATC 1 cut(s) 696
Bsp19I CCATGG 1 cut(s) 394
BspACI CCGC 2 cut(s) 233, 481
BspANI GGCC 1 cut(s) 725
BspHI TCATGA 1 cut(s) 586
BspLI GGNNCC 2 cut(s) 698, 790
BspPI GGATC 2 cut(s) 691, 704
BspQI GCTCTTC 1 cut(s) 851
BsrI ACTGG 1 cut(s) 277
BssECI CCNNGG 1 cut(s) 394
BssMI GATC 1 cut(s) 696
BssSI CACGAG 1 cut(s) 621
BssT1I CCWWGG 1 cut(s) 394
Bst2BI CACGAG 1 cut(s) 621
Bst4CI ACNGT 3 cut(s) 341, 409, 639
Bst6I CTCTTC 1 cut(s) 851
BstAPI GCANNNNNTGC 1 cut(s) 505
BstC8I GCNNGC 1 cut(s) 510
BstDEI CTNAG 2 cut(s) 70, 201
BstDSI CCRYGG 1 cut(s) 394
BstF5I GGATG 2 cut(s) 325, 471
BstKTI GATC 1 cut(s) 699
BstMAI GTCTC 1 cut(s) 412
BstMBI GATC 1 cut(s) 696
BstMWI GCNNNNNNNGC 5 cut(s) 21, 174, 505, 521, 722
BstNSI RCATGY 1 cut(s) 15
BstPAI GACNNNNGTC 1 cut(s) 428
BstSCI CCNGG 1 cut(s) 315
BstSFI CTRYAG 1 cut(s) 691
BstSLI GKGCMC 1 cut(s) 115
BstV1I GCAGC 2 cut(s) 499, 554
BstX2I RGATCY 1 cut(s) 696
BstXI CCANNNNNNTGG 1 cut(s) 491
BstYI RGATCY 1 cut(s) 696
BsuRI GGCC 1 cut(s) 725
BtgI CCRYGG 1 cut(s) 394
BtrI CACGTC 1 cut(s) 736
BtsCI GGATG 2 cut(s) 325, 471
BtsIMutI CAGTG 2 cut(s) 270, 792
Cac8I GCNNGC 1 cut(s) 510
CaiI CAGNNNCTG 1 cut(s) 567
CciI TCATGA 1 cut(s) 586
Cfr13I GGNCC 2 cut(s) 644, 723
CseI GACGC 1 cut(s) 573
Csp6I GTAC 6 cut(s) 41, 67, 74, 128, 144, 539
CviAII CATG 7 cut(s) 12, 19, 395, 412, 587, 727, 766
CviJI RGCY 9 cut(s) 200, 480, 653, 664, 716, 725, 784, 821, 861
CviKI_1 RGCY 9 cut(s) 200, 480, 653, 664, 716, 725, 784, 821, 861
CviQI GTAC 6 cut(s) 41, 67, 74, 128, 144, 539
DdeI CTNAG 2 cut(s) 70, 201
DpnI GATC 1 cut(s) 698
DpnII GATC 1 cut(s) 696
DraIII CACNNNGTG 1 cut(s) 683
Eam1104I CTCTTC 1 cut(s) 851
EarI CTCTTC 1 cut(s) 851
Eco130I CCWWGG 1 cut(s) 394
Eco32I GATATC 1 cut(s) 391
Eco47I GGWCC 1 cut(s) 644
Eco57I CTGAAG 4 cut(s) 154, 656, 800, 807
Eco88I CYCGRG 1 cut(s) 365
EcoRV GATATC 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 394
ErhI CCWWGG 1 cut(s) 394
FaeI CATG 7 cut(s) 15, 22, 398, 415, 590, 730, 769
FalI AAGNNNNNCTT 2 cut(s) 492, 524
FaqI GGGAC 1 cut(s) 416
FatI CATG 7 cut(s) 11, 18, 394, 411, 586, 726, 765
Fnu4HI GCNGC 3 cut(s) 481, 513, 568
FokI GGATG 2 cut(s) 332, 458
Fsp4HI GCNGC 3 cut(s) 481, 513, 568
FspBI CTAG 3 cut(s) 138, 356, 657
GluI GCNGC 3 cut(s) 481, 513, 568
HaeIII GGCC 1 cut(s) 725
HapII CCGG 1 cut(s) 316
HgaI GACGC 1 cut(s) 573
Hin1II CATG 7 cut(s) 15, 22, 398, 415, 590, 730, 769
HindIII AAGCTT 1 cut(s) 782
HinfI GANTC 3 cut(s) 152, 297, 451
HpaII CCGG 1 cut(s) 316
HphI GGTGA 3 cut(s) 272, 427, 695
Hpy166II GTNNAC 2 cut(s) 361, 739
Hpy188I TCNGA 6 cut(s) 173, 190, 388, 563, 775, 826
Hpy188III TCNNGA 4 cut(s) 349, 587, 602, 657
Hpy8I GTNNAC 2 cut(s) 361, 739
HpyAV CCTTC 2 cut(s) 436, 470
HpyCH4III ACNGT 3 cut(s) 341, 409, 639
HpyCH4IV ACGT 2 cut(s) 529, 735
HpyCH4V TGCA 5 cut(s) 54, 161, 311, 524, 805
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 174, 505, 521, 722
HpyF3I CTNAG 2 cut(s) 70, 201
HpySE526I ACGT 2 cut(s) 529, 735
Hsp92II CATG 7 cut(s) 15, 22, 398, 415, 590, 730, 769
Kzo9I GATC 1 cut(s) 696
LguI GCTCTTC 1 cut(s) 851
LmnI GCTCC 1 cut(s) 20
LpnPI CCDG 7 cut(s) 252, 258, 329, 362, 602, 659, 791
Lsp1109I GCAGC 2 cut(s) 499, 554
LweI GCATC 2 cut(s) 101, 181
MaeI CTAG 3 cut(s) 138, 356, 657
MaeII ACGT 2 cut(s) 529, 735
MalI GATC 1 cut(s) 698
MboI GATC 1 cut(s) 696
MboII GAAGA 3 cut(s) 114, 853, 856
MflI RGATCY 1 cut(s) 696
MhlI GDGCHC 1 cut(s) 115
MluCI AATT 5 cut(s) 207, 222, 306, 382, 446
MmeI TCCRAC 1 cut(s) 798
MnlI CCTC 8 cut(s) 246, 287, 488, 547, 594, 620, 624, 700
MroXI GAANNNNTTC 1 cut(s) 450
MseI TTAA 3 cut(s) 62, 123, 243
MspI CCGG 1 cut(s) 316
MspR9I CCNGG 1 cut(s) 317
Mva1269I GAATGC 2 cut(s) 311, 557
MwoI GCNNNNNNNGC 5 cut(s) 21, 174, 505, 521, 722
NciI CCSGG 1 cut(s) 317
NcoI CCATGG 1 cut(s) 394
NdeII GATC 1 cut(s) 696
NlaIII CATG 7 cut(s) 15, 22, 398, 415, 590, 730, 769
NlaIV GGNNCC 2 cut(s) 698, 790
NspI RCATGY 1 cut(s) 15
PagI TCATGA 1 cut(s) 586
PciSI GCTCTTC 1 cut(s) 851
PctI GAATGC 2 cut(s) 311, 557
PdmI GAANNNNTTC 1 cut(s) 450
PfeI GAWTC 3 cut(s) 152, 297, 451
PfoI TCCNGGA 1 cut(s) 315
PkrI GCNGC 3 cut(s) 482, 514, 569
PshAI GACNNNNGTC 1 cut(s) 428
PspN4I GGNNCC 2 cut(s) 698, 790
PspPI GGNCC 2 cut(s) 644, 723
PstNI CAGNNNCTG 1 cut(s) 567
PsuI RGATCY 1 cut(s) 696
RsaI GTAC 6 cut(s) 42, 68, 75, 129, 145, 540
RsaNI GTAC 6 cut(s) 41, 67, 74, 128, 144, 539
SapI GCTCTTC 1 cut(s) 851
SaqAI TTAA 3 cut(s) 62, 123, 243
SatI GCNGC 3 cut(s) 481, 513, 568
Sau3AI GATC 1 cut(s) 696
Sau96I GGNCC 2 cut(s) 644, 723
ScaI AGTACT 1 cut(s) 145
ScrFI CCNGG 1 cut(s) 317
SduI GDGCHC 1 cut(s) 115
SfaNI GCATC 2 cut(s) 101, 181
SfcI CTRYAG 1 cut(s) 691
SinI GGWCC 1 cut(s) 644
SmlI CTYRAG 1 cut(s) 195
SmoI CTYRAG 1 cut(s) 195
Sse9I AATT 5 cut(s) 207, 222, 306, 382, 446
SsiI CCGC 2 cut(s) 233, 481
SspMI CTAG 3 cut(s) 138, 356, 657
StyD4I CCNGG 1 cut(s) 315
StyI CCWWGG 1 cut(s) 394
TaaI ACNGT 3 cut(s) 341, 409, 639
TaiI ACGT 2 cut(s) 532, 738
TaqI TCGA 5 cut(s) 85, 534, 576, 603, 839
TasI AATT 5 cut(s) 207, 222, 306, 382, 446
TatI WGTACW 1 cut(s) 143
TauI GCSGC 1 cut(s) 483
TfiI GAWTC 3 cut(s) 152, 297, 451
Tru1I TTAA 3 cut(s) 62, 123, 243
Tru9I TTAA 3 cut(s) 62, 123, 243
TscAI CASTG 2 cut(s) 277, 799
TseI GCWGC 2 cut(s) 512, 567
TspDTI ATGAA 2 cut(s) 455, 647
TspRI CASTG 2 cut(s) 277, 799
VpaK11BI GGWCC 1 cut(s) 644
XapI RAATTY 1 cut(s) 382
XbaI TCTAGA 1 cut(s) 656
XceI RCATGY 1 cut(s) 15
XmnI GAANNNNTTC 1 cut(s) 450
XspI CTAG 3 cut(s) 138, 356, 657
ZrmI AGTACT 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.