Rh4CG267500

DNA polymerase III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0003855
Physical Location & Seq
Reverse (-)
52173884 .. 52174626
743 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG267500.1

Sequence Viewer

Length: 489 bp
ATGATGAGAACAAGCTCCATGTGCTACTCTAACTTCCAAGTACCCAAGTTTTGCAAAACTTTAAGAAGCATACAATACTCTGATGCTCAAGCTAAGATAATTTTGGACGACAAAATTGTAAAGCCGCCTCCTGTTAATGTGTTGGTTTTTGATATTGAAACCACTGGACTTCACCCAAAGAAAGGAGGAATCATTGAAATCGCATTCCGGGATGTTCAAGGTGGCAAGAACAGTTGTTTCCAGACCCTAGTAAACCCCGAGCAAGATGTTCCAAATTCTGATATCCATGGTATAAGAACTGTCATGGTGAGACATACAGATGTCCCAACGATGAAGGAATTGATTCCATTCCTTCTCCAATACATCCAAAGCCGCCAAGTGCTTGGAGGGCAAGTTTTGCTTGCTGCTCACAATGCAAGACGTTTCGATGTACCCTTTTTGATTGAGGCATTCAGACGCTGCTCATTGGATATTCCTCATGAGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T

Protein Analysis

162

Amino Acids

18.34

Weight (kDa)

8.73

Isoelectric Point (pI)

52.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 48 - 159 4.3e-12 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 125, 373
AcsI RAATTY 1 cut(s) 274
AfaI GTAC 2 cut(s) 42, 432
AfiI CCNNNNNNNGG 1 cut(s) 182
AgsI TTSAA 3 cut(s) 158, 197, 218
AluBI AGCT 2 cut(s) 15, 92
AluI AGCT 2 cut(s) 15, 92
Alw26I GTCTC 1 cut(s) 304
AlwNI CAGNNNCTG 1 cut(s) 459
Ama87I CYCGRG 1 cut(s) 257
ApeKI GCWGC 2 cut(s) 404, 459
ApoI RAATTY 1 cut(s) 274
Asp700I GAANNNNTTC 1 cut(s) 342
AsuC2I CCSGG 1 cut(s) 209
AsuHPI GGTGA 2 cut(s) 164, 319
AvaI CYCGRG 1 cut(s) 257
BbvI GCAGC 2 cut(s) 391, 446
BcnI CCSGG 1 cut(s) 209
BcoDI GTCTC 1 cut(s) 304
BfaI CTAG 1 cut(s) 248
BisI GCNGC 4 cut(s) 125, 373, 405, 460
BlsI GCNGC 4 cut(s) 126, 374, 406, 461
Bme1390I CCNGG 1 cut(s) 209
BmeT110I CYCGRG 1 cut(s) 257
BmrFI CCNGG 1 cut(s) 209
BmsI GCATC 1 cut(s) 73
BpuEI CTTGAG 1 cut(s) 72
BpuMI CCSGG 1 cut(s) 209
BsaJI CCNNGG 1 cut(s) 286
BsaXI ACNNNNNCTCC 2 cut(s) 378, 408
Bsc4I CCNNNNNNNGG 1 cut(s) 182
Bse1I ACTGG 1 cut(s) 169
BseDI CCNNGG 1 cut(s) 286
BseGI GGATG 2 cut(s) 217, 363
BseLI CCNNNNNNNGG 1 cut(s) 182
BseNI ACTGG 1 cut(s) 169
BseXI GCAGC 2 cut(s) 391, 446
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 1 cut(s) 208
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 1 cut(s) 182
BsmAI GTCTC 1 cut(s) 304
BsmFI GGGAC 1 cut(s) 308
BsmI GAATGC 2 cut(s) 203, 449
BsoBI CYCGRG 1 cut(s) 257
Bsp19I CCATGG 1 cut(s) 286
BspACI CCGC 2 cut(s) 125, 373
BspHI TCATGA 2 cut(s) 478, 485
BsrI ACTGG 1 cut(s) 169
BssECI CCNNGG 1 cut(s) 286
BssT1I CCWWGG 1 cut(s) 286
Bst4CI ACNGT 2 cut(s) 233, 301
BstAPI GCANNNNNTGC 1 cut(s) 397
BstC8I GCNNGC 1 cut(s) 402
BstDEI CTNAG 1 cut(s) 93
BstDSI CCRYGG 1 cut(s) 286
BstF5I GGATG 2 cut(s) 217, 363
BstMAI GTCTC 1 cut(s) 304
BstMWI GCNNNNNNNGC 4 cut(s) 21, 388, 397, 413
BstSCI CCNGG 1 cut(s) 207
BstV1I GCAGC 2 cut(s) 391, 446
BstXI CCANNNNNNTGG 1 cut(s) 383
BtgI CCRYGG 1 cut(s) 286
BtsCI GGATG 2 cut(s) 217, 363
BtsIMutI CAGTG 1 cut(s) 162
Cac8I GCNNGC 1 cut(s) 402
CaiI CAGNNNCTG 1 cut(s) 459
CciI TCATGA 2 cut(s) 478, 485
CseI GACGC 1 cut(s) 465
Csp6I GTAC 2 cut(s) 41, 431
CviAII CATG 5 cut(s) 19, 287, 304, 479, 486
CviJI RGCY 4 cut(s) 15, 92, 124, 372
CviKI_1 RGCY 4 cut(s) 15, 92, 124, 372
CviQI GTAC 2 cut(s) 41, 431
DdeI CTNAG 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 286
Eco32I GATATC 1 cut(s) 283
Eco88I CYCGRG 1 cut(s) 257
EcoRV GATATC 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 286
ErhI CCWWGG 1 cut(s) 286
FaeI CATG 5 cut(s) 22, 290, 307, 482, 489
FaiI YATR 8 cut(s) 20, 71, 288, 293, 305, 315, 480, 487
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 1 cut(s) 308
FatI CATG 5 cut(s) 18, 286, 303, 478, 485
Fnu4HI GCNGC 4 cut(s) 125, 373, 405, 460
FokI GGATG 2 cut(s) 224, 350
Fsp4HI GCNGC 4 cut(s) 125, 373, 405, 460
FspBI CTAG 1 cut(s) 248
GluI GCNGC 4 cut(s) 125, 373, 405, 460
HapII CCGG 1 cut(s) 208
HgaI GACGC 1 cut(s) 465
Hin1II CATG 5 cut(s) 22, 290, 307, 482, 489
HinfI GANTC 3 cut(s) 189, 343, 482
HpaII CCGG 1 cut(s) 208
HphI GGTGA 2 cut(s) 164, 319
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 3 cut(s) 82, 280, 455
Hpy188III TCNNGA 3 cut(s) 241, 479, 486
Hpy8I GTNNAC 1 cut(s) 253
HpyAV CCTTC 2 cut(s) 328, 362
HpyCH4III ACNGT 2 cut(s) 233, 301
HpyCH4IV ACGT 1 cut(s) 421
HpyCH4V TGCA 2 cut(s) 54, 416
HpyF10VI GCNNNNNNNGC 4 cut(s) 21, 388, 397, 413
HpyF3I CTNAG 1 cut(s) 93
HpySE526I ACGT 1 cut(s) 421
Hsp92II CATG 5 cut(s) 22, 290, 307, 482, 489
LmnI GCTCC 1 cut(s) 20
LpnPI CCDG 4 cut(s) 144, 150, 221, 254
Lsp1109I GCAGC 2 cut(s) 391, 446
LweI GCATC 1 cut(s) 73
MaeI CTAG 1 cut(s) 248
MaeII ACGT 1 cut(s) 421
MluCI AATT 4 cut(s) 99, 114, 274, 338
MnlI CCTC 5 cut(s) 138, 179, 380, 439, 486
MroXI GAANNNNTTC 1 cut(s) 342
MseI TTAA 2 cut(s) 62, 135
MslI CAYNNNNRTG 1 cut(s) 318
MspI CCGG 1 cut(s) 208
MspR9I CCNGG 1 cut(s) 209
Mva1269I GAATGC 2 cut(s) 203, 449
MwoI GCNNNNNNNGC 4 cut(s) 21, 388, 397, 413
NciI CCSGG 1 cut(s) 209
NcoI CCATGG 1 cut(s) 286
NlaIII CATG 5 cut(s) 22, 290, 307, 482, 489
PagI TCATGA 2 cut(s) 478, 485
PctI GAATGC 2 cut(s) 203, 449
PdmI GAANNNNTTC 1 cut(s) 342
PfeI GAWTC 2 cut(s) 189, 343
PfoI TCCNGGA 1 cut(s) 207
PkrI GCNGC 4 cut(s) 126, 374, 406, 461
PstNI CAGNNNCTG 1 cut(s) 459
RsaI GTAC 2 cut(s) 42, 432
RsaNI GTAC 2 cut(s) 41, 431
RseI CAYNNNNRTG 1 cut(s) 318
SaqAI TTAA 2 cut(s) 62, 135
SatI GCNGC 4 cut(s) 125, 373, 405, 460
ScrFI CCNGG 1 cut(s) 209
SetI ASST 4 cut(s) 17, 94, 223, 424
SfaNI GCATC 1 cut(s) 73
SmiMI CAYNNNNRTG 1 cut(s) 318
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
Sse9I AATT 4 cut(s) 99, 114, 274, 338
SsiI CCGC 2 cut(s) 125, 373
SspMI CTAG 1 cut(s) 248
StyD4I CCNGG 1 cut(s) 207
StyI CCWWGG 1 cut(s) 286
TaaI ACNGT 2 cut(s) 233, 301
TaiI ACGT 1 cut(s) 424
TaqI TCGA 1 cut(s) 426
TasI AATT 4 cut(s) 99, 114, 274, 338
TauI GCSGC 2 cut(s) 127, 375
TfiI GAWTC 2 cut(s) 189, 343
Tru1I TTAA 2 cut(s) 62, 135
Tru9I TTAA 2 cut(s) 62, 135
TscAI CASTG 1 cut(s) 169
TseI GCWGC 2 cut(s) 404, 459
TspDTI ATGAA 1 cut(s) 347
TspRI CASTG 1 cut(s) 169
XapI RAATTY 1 cut(s) 274
XmnI GAANNNNTTC 1 cut(s) 342
XspI CTAG 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.