Rw4G021690

DNA polymerase III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003855
Physical Location & Seq
Reverse (-)
46383565 .. 46384662
1098 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G021690.1

Sequence Viewer

Length: 864 bp
ATGAGAACAAGCTCCATGTGCTACTCTAACTTCCAAGTACCCAAGTTTTGCAAAACTTTAAGGTACTTTGTACCATCTATTCGATTAGCATCTTTAGGAAGAACTTGGGCACAAAACTTTAAGGTACTTACCACTAGTAGTACTACTCATGAACCATCTGCAAACTGCTTCAGGAGCATACAATACTCTGATGCTCAAGCTAAGATAATTTTGGAAGACAAAATTGTAAAGCCGCCTCCTGTTTATGTGTTGGTTTTTGATATTGAAACCACTGGACTTCACCCAAAGAAAGGAGGAATCATTGAAATCGCATTCCGGGATGTTCAAGGTGGCAAGAACAGTTGTTTCCAGACCCTAGTAAACCCCGAGCAAGATGTTTCAAATTCTGATATCCATGGTATAAGAACTGTCATGGTGAGACATACAGATGTCCCAACGATGAAGGAATTGATTCCATTCCTTCTCCAATACATCCAAAGCCGCCAAGTGCTTGGAGGGCAAGTTTTGCTTGCTGCTCACAATGCAAGACGTTTCGATGTACCCTTTTTGATTGAGGCATTCAGACGCTGCTCATTCGATATTCCTCATGACTTGCTGTTTCTCGACACCCTCCCTCTGGCACGAGAAGTGATGAAACTGTATGGACCAAAGGCTTCCAGAAAGCTCTCACTTCAAGCACTTGGGGAGTTCTATAGGATCCCTTTAGAGGGTTCAGCTCATAGGGCCATGACAGACGTGAACTTGCTGACATCTATACTTGCGAACATGATTTCCGACCTGAAGCTTGGTTCCACTGACCTTTTGCAAAGAACTTTCAAGGCTTCAGATGTGAATAACTCGAAGAAGAACGAGAAGAAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T

Protein Analysis

287

Amino Acids

32.38

Weight (kDa)

9.49

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 84 - 250 6.6e-19 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 233, 481
AclWI GGATC 2 cut(s) 691, 704
AcsI RAATTY 1 cut(s) 382
AcuI CTGAAG 3 cut(s) 154, 800, 807
AfaI GTAC 6 cut(s) 39, 65, 72, 126, 142, 540
AfiI CCNNNNNNNGG 4 cut(s) 290, 616, 706, 707
AgsI TTSAA 6 cut(s) 266, 305, 326, 381, 674, 817
AhlI ACTAGT 1 cut(s) 134
AjiI CACGTC 1 cut(s) 736
AloI GAACNNNNNNTCC 2 cut(s) 755, 787
AluBI AGCT 6 cut(s) 12, 200, 664, 716, 784, 861
AluI AGCT 6 cut(s) 12, 200, 664, 716, 784, 861
Alw26I GTCTC 1 cut(s) 412
AlwI GGATC 2 cut(s) 691, 704
AlwNI CAGNNNCTG 1 cut(s) 567
Ama87I CYCGRG 1 cut(s) 365
AoxI GGCC 1 cut(s) 723
ApeKI GCWGC 2 cut(s) 512, 567
ApoI RAATTY 1 cut(s) 382
Asp700I GAANNNNTTC 1 cut(s) 450
AspS9I GGNCC 2 cut(s) 644, 723
AsuC2I CCSGG 1 cut(s) 317
AsuHPI GGTGA 2 cut(s) 272, 427
AvaI CYCGRG 1 cut(s) 365
AvaII GGWCC 1 cut(s) 644
BaeGI GKGCMC 1 cut(s) 112
BamHI GGATCC 1 cut(s) 696
BauI CACGAG 1 cut(s) 621
BbsI GAAGAC 1 cut(s) 222
BbvI GCAGC 2 cut(s) 499, 554
BccI CCATC 2 cut(s) 82, 163
BcnI CCSGG 1 cut(s) 317
BcoDI GTCTC 1 cut(s) 412
BcuI ACTAGT 1 cut(s) 134
BfaI CTAG 2 cut(s) 135, 356
BfmI CTRYAG 1 cut(s) 691
BisI GCNGC 4 cut(s) 233, 481, 513, 568
BlsI GCNGC 4 cut(s) 234, 482, 514, 569
BmcAI AGTACT 1 cut(s) 142
Bme1390I CCNGG 1 cut(s) 317
Bme18I GGWCC 1 cut(s) 644
BmeT110I CYCGRG 1 cut(s) 365
BmgBI CACGTC 1 cut(s) 736
BmgT120I GGNCC 2 cut(s) 644, 723
BmiI GGNNCC 2 cut(s) 698, 790
BmrFI CCNGG 1 cut(s) 317
BmsI GCATC 2 cut(s) 98, 181
BpiI GAAGAC 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 180
BpuMI CCSGG 1 cut(s) 317
BsaBI GATNNNNATC 1 cut(s) 88
BsaJI CCNNGG 1 cut(s) 394
BsaXI ACNNNNNCTCC 2 cut(s) 486, 516
Bsc4I CCNNNNNNNGG 4 cut(s) 290, 616, 706, 707
Bse1I ACTGG 1 cut(s) 277
Bse8I GATNNNNATC 1 cut(s) 88
BseDI CCNNGG 1 cut(s) 394
BseGI GGATG 2 cut(s) 325, 471
BseJI GATNNNNATC 1 cut(s) 88
BseLI CCNNNNNNNGG 4 cut(s) 290, 616, 706, 707
BseNI ACTGG 1 cut(s) 277
BseSI GKGCMC 1 cut(s) 112
BseXI GCAGC 2 cut(s) 499, 554
BshFI GGCC 1 cut(s) 725
BsiHKCI CYCGRG 1 cut(s) 365
BsiSI CCGG 1 cut(s) 316
BslFI GGGAC 1 cut(s) 416
BslI CCNNNNNNNGG 4 cut(s) 290, 616, 706, 707
BsmAI GTCTC 1 cut(s) 412
BsmFI GGGAC 1 cut(s) 416
BsmI GAATGC 2 cut(s) 311, 557
BsnI GGCC 1 cut(s) 725
BsoBI CYCGRG 1 cut(s) 365
Bsp1286I GDGCHC 1 cut(s) 112
Bsp143I GATC 1 cut(s) 696
Bsp19I CCATGG 1 cut(s) 394
BspACI CCGC 2 cut(s) 233, 481
BspANI GGCC 1 cut(s) 725
BspHI TCATGA 2 cut(s) 148, 586
BspLI GGNNCC 2 cut(s) 698, 790
BspPI GGATC 2 cut(s) 691, 704
BspQI GCTCTTC 1 cut(s) 851
BsrI ACTGG 1 cut(s) 277
BssECI CCNNGG 1 cut(s) 394
BssMI GATC 1 cut(s) 696
BssSI CACGAG 1 cut(s) 621
BssT1I CCWWGG 1 cut(s) 394
Bst2BI CACGAG 1 cut(s) 621
Bst4CI ACNGT 3 cut(s) 341, 409, 639
Bst6I CTCTTC 1 cut(s) 851
BstAPI GCANNNNNTGC 1 cut(s) 505
BstC8I GCNNGC 1 cut(s) 510
BstDEI CTNAG 1 cut(s) 201
BstDSI CCRYGG 1 cut(s) 394
BstF5I GGATG 2 cut(s) 325, 471
BstKTI GATC 1 cut(s) 699
BstMAI GTCTC 1 cut(s) 412
BstMBI GATC 1 cut(s) 696
BstMWI GCNNNNNNNGC 6 cut(s) 18, 174, 496, 505, 521, 722
BstSCI CCNGG 1 cut(s) 315
BstSFI CTRYAG 1 cut(s) 691
BstSLI GKGCMC 1 cut(s) 112
BstV1I GCAGC 2 cut(s) 499, 554
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 1 cut(s) 696
BstXI CCANNNNNNTGG 1 cut(s) 491
BstYI RGATCY 1 cut(s) 696
BsuRI GGCC 1 cut(s) 725
BtgI CCRYGG 1 cut(s) 394
BtrI CACGTC 1 cut(s) 736
BtsCI GGATG 2 cut(s) 325, 471
BtsIMutI CAGTG 2 cut(s) 270, 792
Cac8I GCNNGC 1 cut(s) 510
CaiI CAGNNNCTG 1 cut(s) 567
CciI TCATGA 2 cut(s) 148, 586
Cfr13I GGNCC 2 cut(s) 644, 723
CseI GACGC 1 cut(s) 573
Csp6I GTAC 6 cut(s) 38, 64, 71, 125, 141, 539
CviAII CATG 7 cut(s) 16, 149, 395, 412, 587, 727, 766
CviQI GTAC 6 cut(s) 38, 64, 71, 125, 141, 539
DdeI CTNAG 1 cut(s) 201
DpnI GATC 1 cut(s) 698
DpnII GATC 1 cut(s) 696
Eam1104I CTCTTC 1 cut(s) 851
EarI CTCTTC 1 cut(s) 851
Eco130I CCWWGG 1 cut(s) 394
Eco32I GATATC 1 cut(s) 391
Eco47I GGWCC 1 cut(s) 644
Eco57I CTGAAG 3 cut(s) 154, 800, 807
Eco88I CYCGRG 1 cut(s) 365
EcoRV GATATC 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 394
ErhI CCWWGG 1 cut(s) 394
FaeI CATG 7 cut(s) 19, 152, 398, 415, 590, 730, 769
FalI AAGNNNNNCTT 2 cut(s) 492, 524
FaqI GGGAC 1 cut(s) 416
FatI CATG 7 cut(s) 15, 148, 394, 411, 586, 726, 765
Fnu4HI GCNGC 4 cut(s) 233, 481, 513, 568
FokI GGATG 2 cut(s) 332, 458
Fsp4HI GCNGC 4 cut(s) 233, 481, 513, 568
FspBI CTAG 2 cut(s) 135, 356
GluI GCNGC 4 cut(s) 233, 481, 513, 568
HaeIII GGCC 1 cut(s) 725
HapII CCGG 1 cut(s) 316
HgaI GACGC 1 cut(s) 573
Hin1II CATG 7 cut(s) 19, 152, 398, 415, 590, 730, 769
HindIII AAGCTT 1 cut(s) 782
HinfI GANTC 2 cut(s) 297, 451
HpaII CCGG 1 cut(s) 316
HphI GGTGA 2 cut(s) 272, 427
Hpy166II GTNNAC 2 cut(s) 361, 739
Hpy188I TCNGA 5 cut(s) 190, 388, 563, 775, 826
Hpy188III TCNNGA 6 cut(s) 149, 172, 349, 587, 602, 657
Hpy8I GTNNAC 2 cut(s) 361, 739
HpyAV CCTTC 2 cut(s) 436, 470
HpyCH4III ACNGT 3 cut(s) 341, 409, 639
HpyCH4IV ACGT 2 cut(s) 529, 735
HpyCH4V TGCA 4 cut(s) 51, 161, 524, 805
HpyF10VI GCNNNNNNNGC 6 cut(s) 18, 174, 496, 505, 521, 722
HpyF3I CTNAG 1 cut(s) 201
HpySE526I ACGT 2 cut(s) 529, 735
Hsp92II CATG 7 cut(s) 19, 152, 398, 415, 590, 730, 769
Kzo9I GATC 1 cut(s) 696
LguI GCTCTTC 1 cut(s) 851
LmnI GCTCC 2 cut(s) 17, 174
LpnPI CCDG 8 cut(s) 157, 252, 258, 329, 362, 602, 670, 791
Lsp1109I GCAGC 2 cut(s) 499, 554
LweI GCATC 2 cut(s) 98, 181
MaeI CTAG 2 cut(s) 135, 356
MaeII ACGT 2 cut(s) 529, 735
MalI GATC 1 cut(s) 698
MboI GATC 1 cut(s) 696
MboII GAAGA 4 cut(s) 111, 227, 853, 856
MflI RGATCY 1 cut(s) 696
MhlI GDGCHC 1 cut(s) 112
MluCI AATT 4 cut(s) 207, 222, 382, 446
MmeI TCCRAC 1 cut(s) 798
MnlI CCTC 8 cut(s) 246, 287, 488, 547, 594, 620, 624, 700
MroXI GAANNNNTTC 1 cut(s) 450
MseI TTAA 2 cut(s) 59, 120
MslI CAYNNNNRTG 1 cut(s) 426
MspI CCGG 1 cut(s) 316
MspR9I CCNGG 1 cut(s) 317
Mva1269I GAATGC 2 cut(s) 311, 557
MwoI GCNNNNNNNGC 6 cut(s) 18, 174, 496, 505, 521, 722
NciI CCSGG 1 cut(s) 317
NcoI CCATGG 1 cut(s) 394
NdeII GATC 1 cut(s) 696
NlaIII CATG 7 cut(s) 19, 152, 398, 415, 590, 730, 769
NlaIV GGNNCC 2 cut(s) 698, 790
PagI TCATGA 2 cut(s) 148, 586
PciSI GCTCTTC 1 cut(s) 851
PctI GAATGC 2 cut(s) 311, 557
PdmI GAANNNNTTC 1 cut(s) 450
PfeI GAWTC 2 cut(s) 297, 451
PfoI TCCNGGA 1 cut(s) 315
PkrI GCNGC 4 cut(s) 234, 482, 514, 569
PspN4I GGNNCC 2 cut(s) 698, 790
PspPI GGNCC 2 cut(s) 644, 723
PstNI CAGNNNCTG 1 cut(s) 567
PsuI RGATCY 1 cut(s) 696
RsaI GTAC 6 cut(s) 39, 65, 72, 126, 142, 540
RsaNI GTAC 6 cut(s) 38, 64, 71, 125, 141, 539
RseI CAYNNNNRTG 1 cut(s) 426
SapI GCTCTTC 1 cut(s) 851
SaqAI TTAA 2 cut(s) 59, 120
SatI GCNGC 4 cut(s) 233, 481, 513, 568
Sau3AI GATC 1 cut(s) 696
Sau96I GGNCC 2 cut(s) 644, 723
ScaI AGTACT 1 cut(s) 142
ScrFI CCNGG 1 cut(s) 317
SduI GDGCHC 1 cut(s) 112
SfaNI GCATC 2 cut(s) 98, 181
SfcI CTRYAG 1 cut(s) 691
SinI GGWCC 1 cut(s) 644
SmiMI CAYNNNNRTG 1 cut(s) 426
SmlI CTYRAG 1 cut(s) 195
SmoI CTYRAG 1 cut(s) 195
SpeI ACTAGT 1 cut(s) 134
Sse9I AATT 4 cut(s) 207, 222, 382, 446
SsiI CCGC 2 cut(s) 233, 481
SspMI CTAG 2 cut(s) 135, 356
StyD4I CCNGG 1 cut(s) 315
StyI CCWWGG 1 cut(s) 394
TaaI ACNGT 3 cut(s) 341, 409, 639
TaiI ACGT 2 cut(s) 532, 738
TaqI TCGA 5 cut(s) 82, 534, 576, 603, 839
TasI AATT 4 cut(s) 207, 222, 382, 446
TatI WGTACW 1 cut(s) 140
TauI GCSGC 2 cut(s) 235, 483
TfiI GAWTC 2 cut(s) 297, 451
Tru1I TTAA 2 cut(s) 59, 120
Tru9I TTAA 2 cut(s) 59, 120
TscAI CASTG 2 cut(s) 277, 799
TseI GCWGC 2 cut(s) 512, 567
TspDTI ATGAA 3 cut(s) 165, 455, 647
TspRI CASTG 2 cut(s) 277, 799
VpaK11BI GGWCC 1 cut(s) 644
XapI RAATTY 1 cut(s) 382
XmnI GAANNNNTTC 1 cut(s) 450
XspI CTAG 2 cut(s) 135, 356
ZrmI AGTACT 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.