Rorug04G0193700

DNA polymerase III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003855
Physical Location & Seq
Reverse (-)
33752267 .. 33752953
687 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0193700.1

Sequence Viewer

Length: 600 bp
ATGCCTCCCGAGCTTTTCGATAAACTTCTTGTTATGATGTGGAGTATCTGGAGGAATCAGAATGAGAAGTTGTGGAGGGATCAGACTCAAACAGGGTTGGGGGTTGCGGCCTCTGCAATGGCCTGGTTTGAGGAGTATTCCCGAGTCCACAGGGATGCTGCACCGTTGGTGCGAAGTCATCCTCCTCGTAGGAAGAAATGGATGGCACCAATGGAAGATACGCTTAAGTTAAACGTGGATGGGGCTTTCCTTCCAACTGTTCAGTATGGCGGTACAGGTGGAGTGTTAAGGAATGCCCATAGAGGTTTCATTGCTGCGTTCTCTTACAAGTCAGCTTTCGTTACCTCTCCATTGCATACTGAACTGGCTATCAATTCCATGGCTGAGGATCTTTCTGATTTCGCTGCTTTGATTTCTGATATCAAAGGTTTAGTTAATGCCATGGGGGAGATTGTAATCAGTTTTACTCCTAGACAAGCAAATATGGTTGCTCATCGTCTAGCTAGTCATAGTTTTGATTCAAATGTCCATCTTGAATGGTTTACTACTGCTCTAGAGTTTATACTGGATGCCCTCATGTACGATTGTAATCGTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T

Protein Analysis

199

Amino Acids

22.49

Weight (kDa)

6.37

Isoelectric Point (pI)

32.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 205
AciI CCGC 2 cut(s) 107, 270
AclWI GGATC 2 cut(s) 87, 396
AfaI GTAC 2 cut(s) 274, 581
AflII CTTAAG 1 cut(s) 224
AgsI TTSAA 2 cut(s) 522, 536
AjnI CCWGG 1 cut(s) 122
AluBI AGCT 3 cut(s) 13, 335, 503
AluI AGCT 3 cut(s) 13, 335, 503
AlwI GGATC 2 cut(s) 87, 396
Ama87I CYCGRG 2 cut(s) 8, 141
AoxI GGCC 2 cut(s) 108, 120
ApeKI GCWGC 3 cut(s) 158, 314, 404
AvaI CYCGRG 2 cut(s) 8, 141
BanI GGYRCC 1 cut(s) 205
BbvCI CCTCAGC 1 cut(s) 384
BbvI GCAGC 3 cut(s) 145, 301, 391
BccI CCATC 3 cut(s) 196, 233, 537
BciT130I CCWGG 1 cut(s) 124
BfaI CTAG 5 cut(s) 471, 500, 504, 554, 598
BfrI CTTAAG 1 cut(s) 224
BisI GCNGC 4 cut(s) 108, 159, 315, 405
BlsI GCNGC 4 cut(s) 109, 160, 316, 406
Bme1390I CCNGG 1 cut(s) 124
BmeT110I CYCGRG 2 cut(s) 8, 141
BmiI GGNNCC 1 cut(s) 207
BmrFI CCNGG 1 cut(s) 124
BmsI GCATC 2 cut(s) 145, 559
BpmI CTGGAG 1 cut(s) 70
Bpu10I CCTNAGC 1 cut(s) 384
BsaBI GATNNNNATC 2 cut(s) 455, 588
BsaJI CCNNGG 2 cut(s) 378, 441
Bse1I ACTGG 2 cut(s) 369, 570
Bse3DI GCAATG 3 cut(s) 123, 309, 350
Bse8I GATNNNNATC 2 cut(s) 455, 588
BseBI CCWGG 1 cut(s) 124
BseDI CCNNGG 2 cut(s) 378, 441
BseGI GGATG 5 cut(s) 160, 178, 207, 244, 574
BseJI GATNNNNATC 2 cut(s) 455, 588
BseMI GCAATG 3 cut(s) 123, 309, 350
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 2 cut(s) 369, 570
BseRI GAGGAG 2 cut(s) 146, 174
BseXI GCAGC 3 cut(s) 145, 301, 391
BsgI GTGCAG 1 cut(s) 144
BshFI GGCC 2 cut(s) 110, 122
BshNI GGYRCC 1 cut(s) 205
BsiHKCI CYCGRG 2 cut(s) 8, 141
BsmI GAATGC 1 cut(s) 298
BsnI GGCC 2 cut(s) 110, 122
BsoBI CYCGRG 2 cut(s) 8, 141
Bsp143I GATC 2 cut(s) 79, 388
Bsp19I CCATGG 2 cut(s) 378, 441
BspACI CCGC 2 cut(s) 107, 270
BspANI GGCC 2 cut(s) 110, 122
BspCNI CTCAG 1 cut(s) 376
BspLI GGNNCC 1 cut(s) 207
BspPI GGATC 2 cut(s) 87, 396
BspT107I GGYRCC 1 cut(s) 205
BspTI CTTAAG 1 cut(s) 224
BsrDI GCAATG 3 cut(s) 123, 309, 350
BsrI ACTGG 2 cut(s) 369, 570
BssECI CCNNGG 2 cut(s) 378, 441
BssMI GATC 2 cut(s) 79, 388
BssT1I CCWWGG 2 cut(s) 378, 441
Bst2UI CCWGG 1 cut(s) 124
Bst4CI ACNGT 2 cut(s) 165, 259
BstAFI CTTAAG 1 cut(s) 224
BstDEI CTNAG 1 cut(s) 384
BstDSI CCRYGG 2 cut(s) 378, 441
BstF5I GGATG 5 cut(s) 160, 178, 207, 244, 574
BstKTI GATC 2 cut(s) 82, 391
BstMBI GATC 2 cut(s) 79, 388
BstMWI GCNNNNNNNGC 2 cut(s) 10, 113
BstNI CCWGG 1 cut(s) 124
BstSCI CCNGG 1 cut(s) 122
BstV1I GCAGC 3 cut(s) 145, 301, 391
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
BsuRI GGCC 2 cut(s) 110, 122
BtgI CCRYGG 2 cut(s) 378, 441
BtsCI GGATG 5 cut(s) 160, 178, 207, 244, 574
Csp6I GTAC 2 cut(s) 273, 580
CviAII CATG 3 cut(s) 379, 442, 577
CviJI RGCY 8 cut(s) 13, 110, 122, 245, 335, 368, 383, 503
CviKI_1 RGCY 8 cut(s) 13, 110, 122, 245, 335, 368, 383, 503
CviQI GTAC 2 cut(s) 273, 580
DdeI CTNAG 1 cut(s) 384
DpnI GATC 2 cut(s) 81, 390
DpnII GATC 2 cut(s) 79, 388
Eco130I CCWWGG 2 cut(s) 378, 441
Eco32I GATATC 1 cut(s) 421
Eco88I CYCGRG 2 cut(s) 8, 141
EcoRII CCWGG 1 cut(s) 122
EcoRV GATATC 1 cut(s) 421
EcoT14I CCWWGG 2 cut(s) 378, 441
ErhI CCWWGG 2 cut(s) 378, 441
FaeI CATG 3 cut(s) 382, 445, 580
FalI AAGNNNNNCTT 2 cut(s) 207, 239
FatI CATG 3 cut(s) 378, 441, 576
Fnu4HI GCNGC 4 cut(s) 108, 159, 315, 405
FokI GGATG 5 cut(s) 165, 167, 214, 251, 581
Fsp4HI GCNGC 4 cut(s) 108, 159, 315, 405
FspBI CTAG 5 cut(s) 471, 500, 504, 554, 598
GluI GCNGC 4 cut(s) 108, 159, 315, 405
GsuI CTGGAG 1 cut(s) 70
HaeIII GGCC 2 cut(s) 110, 122
Hin1II CATG 3 cut(s) 382, 445, 580
HinfI GANTC 4 cut(s) 55, 85, 144, 518
Hpy166II GTNNAC 2 cut(s) 148, 543
Hpy188I TCNGA 4 cut(s) 60, 84, 397, 418
Hpy188III TCNNGA 5 cut(s) 8, 49, 141, 533, 554
Hpy8I GTNNAC 2 cut(s) 148, 543
HpyAV CCTTC 1 cut(s) 260
HpyCH4III ACNGT 2 cut(s) 165, 259
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 3 cut(s) 116, 161, 355
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 113
HpyF3I CTNAG 1 cut(s) 384
HpySE526I ACGT 1 cut(s) 234
Hsp92II CATG 3 cut(s) 382, 445, 580
Kzo9I GATC 2 cut(s) 79, 388
LpnPI CCDG 8 cut(s) 34, 78, 109, 136, 136, 261, 350, 551
Lsp1109I GCAGC 3 cut(s) 145, 301, 391
LweI GCATC 2 cut(s) 145, 559
MaeI CTAG 5 cut(s) 471, 500, 504, 554, 598
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 1 cut(s) 340
MalI GATC 2 cut(s) 81, 390
MboI GATC 2 cut(s) 79, 388
MboII GAAGA 2 cut(s) 205, 227
MflI RGATCY 1 cut(s) 388
MluCI AATT 1 cut(s) 373
MlyI GAGTC 2 cut(s) 79, 153
MmeI TCCRAC 1 cut(s) 278
MseI TTAA 4 cut(s) 225, 230, 287, 435
MslI CAYNNNNRTG 1 cut(s) 153
MspCI CTTAAG 1 cut(s) 224
MspR9I CCNGG 1 cut(s) 124
Mva1269I GAATGC 1 cut(s) 298
MvaI CCWGG 1 cut(s) 124
MwoI GCNNNNNNNGC 2 cut(s) 10, 113
NcoI CCATGG 2 cut(s) 378, 441
NdeII GATC 2 cut(s) 79, 388
NlaIII CATG 3 cut(s) 382, 445, 580
NlaIV GGNNCC 1 cut(s) 207
PctI GAATGC 1 cut(s) 298
PfeI GAWTC 2 cut(s) 55, 518
PkrI GCNGC 4 cut(s) 109, 160, 316, 406
PleI GAGTC 2 cut(s) 79, 152
PpsI GAGTC 2 cut(s) 79, 152
Psp6I CCWGG 1 cut(s) 122
PspGI CCWGG 1 cut(s) 122
PspN4I GGNNCC 1 cut(s) 207
PsuI RGATCY 1 cut(s) 388
RsaI GTAC 2 cut(s) 274, 581
RsaNI GTAC 2 cut(s) 273, 580
RseI CAYNNNNRTG 1 cut(s) 153
SaqAI TTAA 4 cut(s) 225, 230, 287, 435
SatI GCNGC 4 cut(s) 108, 159, 315, 405
Sau3AI GATC 2 cut(s) 79, 388
SchI GAGTC 2 cut(s) 79, 153
ScrFI CCNGG 1 cut(s) 124
SetI ASST 8 cut(s) 15, 237, 280, 307, 337, 347, 430, 505
SfaNI GCATC 2 cut(s) 145, 559
SmiMI CAYNNNNRTG 1 cut(s) 153
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
Sse9I AATT 1 cut(s) 373
SsiI CCGC 2 cut(s) 107, 270
SspMI CTAG 5 cut(s) 471, 500, 504, 554, 598
StyD4I CCNGG 1 cut(s) 122
StyI CCWWGG 2 cut(s) 378, 441
TaaI ACNGT 2 cut(s) 165, 259
TaiI ACGT 1 cut(s) 237
TaqI TCGA 1 cut(s) 18
TasI AATT 1 cut(s) 373
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 2 cut(s) 55, 518
Tru1I TTAA 4 cut(s) 225, 230, 287, 435
Tru9I TTAA 4 cut(s) 225, 230, 287, 435
TseI GCWGC 3 cut(s) 158, 314, 404
TspDTI ATGAA 1 cut(s) 298
Vha464I CTTAAG 1 cut(s) 224
XbaI TCTAGA 1 cut(s) 553
XspI CTAG 5 cut(s) 471, 500, 504, 554, 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.