Rh4DG251300

DNA polymerase III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0003855
Physical Location & Seq
Reverse (-)
46615916 .. 46617016
1101 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG251300.1

Sequence Viewer

Length: 756 bp
ATGATGAGAACAAGCTCCATGTGCTACTCTAACTTCCAAGTACCCAAGTTTTGCAAAACTTTAAGGAGCATACAATACTCTGATGCTCAAGCTAAGATAATTTTGGAAGACAAAATTGTAAAGCCGCCTCCTGTTAATGTGTTGGTTTTTGATATTGAAACCACTGGACTTCACCCAAAGAAAGGAGGAATCATTGAAATCGCATTCCGGGATGTTCAAGGTGGCAAGAACAGTTGTTTCCAGACCCTAGTAAACCCCGAGCAAGATGTTTCAAATTCTGATATCCATGGTATAAGAACTGTCATGGTGAGACATACAGATGTCCCAACGATGAAGGAATTGATTCCATTCCTTCTCCAATACATCCAAAGCCGCCAAGTGCTTGGAGGGCAAGTTTTGCTTGCTGCTCACAATGCAAGACGTTTCGATGTACCCTTTTTGATTGAGGCATTCAGACGCTGCTCATTCGATATTCCTCATGACTTGCTGTTTCTCGACACCCTCCCTCTGGCACGAGAAGTGATGAAACTGTATGGACCAAAGGCTTCTAGAAAGCTCTCACTTCAAGCACTTGGTGAGTTCTATAGGATCCCTTTAGAGGGTTCAGCTCATAGGGCCATGACAGACGTGAACTTGCTGACATCTATACTTGCGAACATGATTTCCGACCTGAAGCTTGGTTCCACTGACCTTTTGCAAAGAACTTTCAAGGCTTCAGATGTGAATAACTCGAAGAAGAACGAGAAGAAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T

Protein Analysis

251

Amino Acids

28.29

Weight (kDa)

9.35

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 48 - 214 4.4e-19 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 125, 373
AclWI GGATC 2 cut(s) 583, 596
AcsI RAATTY 1 cut(s) 274
AcuI CTGAAG 2 cut(s) 692, 699
AdeI CACNNNGTG 1 cut(s) 575
AfaI GTAC 2 cut(s) 42, 432
AfiI CCNNNNNNNGG 4 cut(s) 182, 508, 598, 599
AgsI TTSAA 6 cut(s) 158, 197, 218, 273, 566, 709
AjiI CACGTC 1 cut(s) 628
AloI GAACNNNNNNTCC 2 cut(s) 647, 679
AluBI AGCT 6 cut(s) 15, 92, 556, 608, 676, 753
AluI AGCT 6 cut(s) 15, 92, 556, 608, 676, 753
Alw26I GTCTC 1 cut(s) 304
AlwI GGATC 2 cut(s) 583, 596
AlwNI CAGNNNCTG 1 cut(s) 459
Ama87I CYCGRG 1 cut(s) 257
AoxI GGCC 1 cut(s) 615
ApeKI GCWGC 2 cut(s) 404, 459
ApoI RAATTY 1 cut(s) 274
Asp700I GAANNNNTTC 1 cut(s) 342
AspS9I GGNCC 2 cut(s) 536, 615
AsuC2I CCSGG 1 cut(s) 209
AsuHPI GGTGA 3 cut(s) 164, 319, 587
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 1 cut(s) 536
BamHI GGATCC 1 cut(s) 588
BauI CACGAG 1 cut(s) 513
BbsI GAAGAC 1 cut(s) 114
BbvI GCAGC 2 cut(s) 391, 446
BcnI CCSGG 1 cut(s) 209
BcoDI GTCTC 1 cut(s) 304
BfaI CTAG 2 cut(s) 248, 549
BfmI CTRYAG 1 cut(s) 583
BisI GCNGC 4 cut(s) 125, 373, 405, 460
BlsI GCNGC 4 cut(s) 126, 374, 406, 461
Bme1390I CCNGG 1 cut(s) 209
Bme18I GGWCC 1 cut(s) 536
BmeT110I CYCGRG 1 cut(s) 257
BmgBI CACGTC 1 cut(s) 628
BmgT120I GGNCC 2 cut(s) 536, 615
BmiI GGNNCC 2 cut(s) 590, 682
BmrFI CCNGG 1 cut(s) 209
BmsI GCATC 1 cut(s) 73
BpiI GAAGAC 1 cut(s) 114
BpuEI CTTGAG 1 cut(s) 72
BpuMI CCSGG 1 cut(s) 209
BsaJI CCNNGG 1 cut(s) 286
BsaXI ACNNNNNCTCC 2 cut(s) 378, 408
Bsc4I CCNNNNNNNGG 4 cut(s) 182, 508, 598, 599
Bse1I ACTGG 1 cut(s) 169
BseDI CCNNGG 1 cut(s) 286
BseGI GGATG 2 cut(s) 217, 363
BseLI CCNNNNNNNGG 4 cut(s) 182, 508, 598, 599
BseNI ACTGG 1 cut(s) 169
BseXI GCAGC 2 cut(s) 391, 446
BshFI GGCC 1 cut(s) 617
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 1 cut(s) 208
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 4 cut(s) 182, 508, 598, 599
BsmAI GTCTC 1 cut(s) 304
BsmFI GGGAC 1 cut(s) 308
BsmI GAATGC 2 cut(s) 203, 449
BsnI GGCC 1 cut(s) 617
BsoBI CYCGRG 1 cut(s) 257
Bsp143I GATC 1 cut(s) 588
Bsp19I CCATGG 1 cut(s) 286
BspACI CCGC 2 cut(s) 125, 373
BspANI GGCC 1 cut(s) 617
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 2 cut(s) 590, 682
BspPI GGATC 2 cut(s) 583, 596
BspQI GCTCTTC 1 cut(s) 743
BsrI ACTGG 1 cut(s) 169
BssECI CCNNGG 1 cut(s) 286
BssMI GATC 1 cut(s) 588
BssSI CACGAG 1 cut(s) 513
BssT1I CCWWGG 1 cut(s) 286
Bst2BI CACGAG 1 cut(s) 513
Bst4CI ACNGT 3 cut(s) 233, 301, 531
Bst6I CTCTTC 1 cut(s) 743
BstAPI GCANNNNNTGC 1 cut(s) 397
BstC8I GCNNGC 1 cut(s) 402
BstDEI CTNAG 1 cut(s) 93
BstDSI CCRYGG 1 cut(s) 286
BstF5I GGATG 2 cut(s) 217, 363
BstKTI GATC 1 cut(s) 591
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 1 cut(s) 588
BstMWI GCNNNNNNNGC 5 cut(s) 21, 388, 397, 413, 614
BstSCI CCNGG 1 cut(s) 207
BstSFI CTRYAG 1 cut(s) 583
BstV1I GCAGC 2 cut(s) 391, 446
BstV2I GAAGAC 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 588
BstXI CCANNNNNNTGG 1 cut(s) 383
BstYI RGATCY 1 cut(s) 588
BsuRI GGCC 1 cut(s) 617
BtgI CCRYGG 1 cut(s) 286
BtrI CACGTC 1 cut(s) 628
BtsCI GGATG 2 cut(s) 217, 363
BtsIMutI CAGTG 2 cut(s) 162, 684
Cac8I GCNNGC 1 cut(s) 402
CaiI CAGNNNCTG 1 cut(s) 459
CciI TCATGA 1 cut(s) 478
Cfr13I GGNCC 2 cut(s) 536, 615
CseI GACGC 1 cut(s) 465
Csp6I GTAC 2 cut(s) 41, 431
CviAII CATG 6 cut(s) 19, 287, 304, 479, 619, 658
CviQI GTAC 2 cut(s) 41, 431
DdeI CTNAG 1 cut(s) 93
DpnI GATC 1 cut(s) 590
DpnII GATC 1 cut(s) 588
DraIII CACNNNGTG 1 cut(s) 575
Eam1104I CTCTTC 1 cut(s) 743
EarI CTCTTC 1 cut(s) 743
Eco130I CCWWGG 1 cut(s) 286
Eco32I GATATC 1 cut(s) 283
Eco47I GGWCC 1 cut(s) 536
Eco57I CTGAAG 2 cut(s) 692, 699
Eco88I CYCGRG 1 cut(s) 257
EcoRV GATATC 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 286
ErhI CCWWGG 1 cut(s) 286
FaeI CATG 6 cut(s) 22, 290, 307, 482, 622, 661
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 1 cut(s) 308
FatI CATG 6 cut(s) 18, 286, 303, 478, 618, 657
Fnu4HI GCNGC 4 cut(s) 125, 373, 405, 460
FokI GGATG 2 cut(s) 224, 350
Fsp4HI GCNGC 4 cut(s) 125, 373, 405, 460
FspBI CTAG 2 cut(s) 248, 549
GluI GCNGC 4 cut(s) 125, 373, 405, 460
HaeIII GGCC 1 cut(s) 617
HapII CCGG 1 cut(s) 208
HgaI GACGC 1 cut(s) 465
Hin1II CATG 6 cut(s) 22, 290, 307, 482, 622, 661
HindIII AAGCTT 1 cut(s) 674
HinfI GANTC 2 cut(s) 189, 343
HpaII CCGG 1 cut(s) 208
HphI GGTGA 3 cut(s) 164, 319, 587
Hpy166II GTNNAC 2 cut(s) 253, 631
Hpy188I TCNGA 5 cut(s) 82, 280, 455, 667, 718
Hpy188III TCNNGA 4 cut(s) 241, 479, 494, 549
Hpy8I GTNNAC 2 cut(s) 253, 631
HpyAV CCTTC 2 cut(s) 328, 362
HpyCH4III ACNGT 3 cut(s) 233, 301, 531
HpyCH4IV ACGT 2 cut(s) 421, 627
HpyCH4V TGCA 3 cut(s) 54, 416, 697
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 388, 397, 413, 614
HpyF3I CTNAG 1 cut(s) 93
HpySE526I ACGT 2 cut(s) 421, 627
Hsp92II CATG 6 cut(s) 22, 290, 307, 482, 622, 661
Kzo9I GATC 1 cut(s) 588
LguI GCTCTTC 1 cut(s) 743
LmnI GCTCC 2 cut(s) 20, 66
LpnPI CCDG 6 cut(s) 144, 150, 221, 254, 494, 683
Lsp1109I GCAGC 2 cut(s) 391, 446
LweI GCATC 1 cut(s) 73
MaeI CTAG 2 cut(s) 248, 549
MaeII ACGT 2 cut(s) 421, 627
MalI GATC 1 cut(s) 590
MboI GATC 1 cut(s) 588
MboII GAAGA 3 cut(s) 119, 745, 748
MflI RGATCY 1 cut(s) 588
MluCI AATT 4 cut(s) 99, 114, 274, 338
MmeI TCCRAC 1 cut(s) 690
MnlI CCTC 8 cut(s) 138, 179, 380, 439, 486, 512, 516, 592
MroXI GAANNNNTTC 1 cut(s) 342
MseI TTAA 2 cut(s) 62, 135
MslI CAYNNNNRTG 1 cut(s) 318
MspI CCGG 1 cut(s) 208
MspR9I CCNGG 1 cut(s) 209
Mva1269I GAATGC 2 cut(s) 203, 449
MwoI GCNNNNNNNGC 5 cut(s) 21, 388, 397, 413, 614
NciI CCSGG 1 cut(s) 209
NcoI CCATGG 1 cut(s) 286
NdeII GATC 1 cut(s) 588
NlaIII CATG 6 cut(s) 22, 290, 307, 482, 622, 661
NlaIV GGNNCC 2 cut(s) 590, 682
PagI TCATGA 1 cut(s) 478
PciSI GCTCTTC 1 cut(s) 743
PctI GAATGC 2 cut(s) 203, 449
PdmI GAANNNNTTC 1 cut(s) 342
PfeI GAWTC 2 cut(s) 189, 343
PfoI TCCNGGA 1 cut(s) 207
PkrI GCNGC 4 cut(s) 126, 374, 406, 461
PspN4I GGNNCC 2 cut(s) 590, 682
PspPI GGNCC 2 cut(s) 536, 615
PstNI CAGNNNCTG 1 cut(s) 459
PsuI RGATCY 1 cut(s) 588
RsaI GTAC 2 cut(s) 42, 432
RsaNI GTAC 2 cut(s) 41, 431
RseI CAYNNNNRTG 1 cut(s) 318
SapI GCTCTTC 1 cut(s) 743
SaqAI TTAA 2 cut(s) 62, 135
SatI GCNGC 4 cut(s) 125, 373, 405, 460
Sau3AI GATC 1 cut(s) 588
Sau96I GGNCC 2 cut(s) 536, 615
ScrFI CCNGG 1 cut(s) 209
SfaNI GCATC 1 cut(s) 73
SfcI CTRYAG 1 cut(s) 583
SinI GGWCC 1 cut(s) 536
SmiMI CAYNNNNRTG 1 cut(s) 318
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
Sse9I AATT 4 cut(s) 99, 114, 274, 338
SsiI CCGC 2 cut(s) 125, 373
SspMI CTAG 2 cut(s) 248, 549
StyD4I CCNGG 1 cut(s) 207
StyI CCWWGG 1 cut(s) 286
TaaI ACNGT 3 cut(s) 233, 301, 531
TaiI ACGT 2 cut(s) 424, 630
TaqI TCGA 4 cut(s) 426, 468, 495, 731
TasI AATT 4 cut(s) 99, 114, 274, 338
TauI GCSGC 2 cut(s) 127, 375
TfiI GAWTC 2 cut(s) 189, 343
Tru1I TTAA 2 cut(s) 62, 135
Tru9I TTAA 2 cut(s) 62, 135
TscAI CASTG 2 cut(s) 169, 691
TseI GCWGC 2 cut(s) 404, 459
TspDTI ATGAA 2 cut(s) 347, 539
TspRI CASTG 2 cut(s) 169, 691
VpaK11BI GGWCC 1 cut(s) 536
XapI RAATTY 1 cut(s) 274
XbaI TCTAGA 1 cut(s) 548
XmnI GAANNNNTTC 1 cut(s) 342
XspI CTAG 2 cut(s) 248, 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.