pycom13g13620

DNA polymerase III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003855
Physical Location & Seq
Forward (+)
9676063 .. 9677173
1111 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g13620.3

Sequence Viewer

Length: 804 bp
ATGAGGGCGTGCGCCATGTACTGTTCTAACTTGCAGGTACCTAAGTTTGGAGTTCAGTCACCATCTCTTCGATTGGCATCTTTACGAAGAAGACTAACAACAACAACCGCTTTAAGCAAATCACCTTCGAAATCCTTCAGAAGCATACAATACTCCGATGCTCAAGCAACGATTTTGGATTTCGGAGTTTTCAAGGATCTTGTGAAAATATTTGTTCTAGACCTTGAAACCACCGGACTTTGCAGAAGGATAGGACATGTAACTGAAATCGCACTCCGCGATCTTCAAGGCGGCAAGAACAGTTGCTTTCAAACCCTGGTGAACCCTCAACAACGTGTTTCAAATTCTAGCATCCACGGTATAACAACCGACATGGTGACACATTCAGATGTCCCGAGGATGGTGGAGTTGATTCCGATTCTTCTCGAATACATCAGAAGCCGGCAAGGACCTGGAGTGCAAGTTTTATTTGCAGCTCACAATGCACGGCGTTTCGACGTGCCCTTCTTAATCAAGGAATTCAGCTCCTGCTCGTTTGAAATTCCTCCTGACTGGCTGTTTCTGGACACCCTCGCACTGTCGCATGAGTTAAGGAAATTATACGGGCCAAAGGTTCCTCCAAAGATCTCTCTTGAGGGACTTAGTGAGTTCTACAAGATCACTAGAGAAGGTCCAAAACACAGGGCCATGGCAGATGTGAACTTGCTGACATCTATACTTCCAAACATGACTTCTGACCTCAACCTCGGAGTCGCGGACCTTTTGCAGAGGAGTTTCAAGGCTTCTGTCTGCAGTTTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T

Protein Analysis

268

Amino Acids

29.86

Weight (kDa)

9.41

Isoelectric Point (pI)

51.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 25
Acc65I GGTACC 1 cut(s) 37
AccB1I GGYRCC 1 cut(s) 37
AccII CGCG 2 cut(s) 279, 755
AciI CCGC 4 cut(s) 108, 277, 291, 755
AclWI GGATC 1 cut(s) 204
AcsI RAATTY 3 cut(s) 343, 518, 540
AcuI CTGAAG 1 cut(s) 121
AfaI GTAC 2 cut(s) 20, 39
AfiI CCNNNNNNNGG 2 cut(s) 47, 400
AflIII ACRYGT 2 cut(s) 256, 334
AgsI TTSAA 7 cut(s) 193, 227, 287, 311, 342, 539, 778
AjiI CACGTC 1 cut(s) 499
AjnI CCWGG 2 cut(s) 315, 451
AjuI GAANNNNNNNTTGG 2 cut(s) 715, 747
AluBI AGCT 2 cut(s) 476, 525
AluI AGCT 2 cut(s) 476, 525
AlwI GGATC 1 cut(s) 204
AlwNI CAGNNNCTG 1 cut(s) 528
Ama87I CYCGRG 1 cut(s) 394
AoxI GGCC 2 cut(s) 605, 684
ApeKI GCWGC 1 cut(s) 473
ApoI RAATTY 3 cut(s) 343, 518, 540
Asp700I GAANNNNTTC 1 cut(s) 134
Asp718I GGTACC 1 cut(s) 37
AspLEI GCGC 1 cut(s) 14
AspS9I GGNCC 5 cut(s) 449, 605, 671, 684, 757
AsuHPI GGTGA 4 cut(s) 51, 114, 331, 388
AsuII TTCGAA 1 cut(s) 128
AvaI CYCGRG 1 cut(s) 394
AvaII GGWCC 3 cut(s) 449, 671, 757
BaeGI GKGCMC 1 cut(s) 504
BaeI ACNNNNGTAYC 2 cut(s) 29, 62
BanI GGYRCC 1 cut(s) 37
BbsI GAAGAC 1 cut(s) 97
BbvI GCAGC 1 cut(s) 485
BccI CCATC 2 cut(s) 70, 394
BceAI ACGGC 1 cut(s) 503
BciT130I CCWGG 2 cut(s) 317, 453
BfaI CTAG 3 cut(s) 218, 348, 663
BfmI CTRYAG 1 cut(s) 790
BfuAI ACCTGC 1 cut(s) 25
BglII AGATCT 1 cut(s) 624
BisI GCNGC 2 cut(s) 292, 474
BlsI GCNGC 2 cut(s) 293, 475
Bme1390I CCNGG 2 cut(s) 317, 453
Bme18I GGWCC 3 cut(s) 449, 671, 757
BmeT110I CYCGRG 1 cut(s) 394
BmgBI CACGTC 1 cut(s) 499
BmgT120I GGNCC 5 cut(s) 449, 605, 671, 684, 757
BmiI GGNNCC 2 cut(s) 39, 615
BmrFI CCNGG 2 cut(s) 317, 453
BmsI GCATC 3 cut(s) 86, 148, 360
BpiI GAAGAC 1 cut(s) 97
BpmI CTGGAG 1 cut(s) 474
Bpu14I TTCGAA 1 cut(s) 128
BpuEI CTTGAG 2 cut(s) 147, 653
BsaBI GATNNNNATC 1 cut(s) 76
BsaJI CCNNGG 5 cut(s) 315, 355, 395, 687, 745
BsaWI WCCGGW 1 cut(s) 233
BsaXI ACNNNNNCTCC 2 cut(s) 447, 477
Bsc4I CCNNNNNNNGG 2 cut(s) 47, 400
Bse118I RCCGGY 1 cut(s) 441
Bse1I ACTGG 1 cut(s) 557
Bse8I GATNNNNATC 1 cut(s) 76
BseBI CCWGG 2 cut(s) 317, 453
BseDI CCNNGG 5 cut(s) 315, 355, 395, 687, 745
BseGI GGATG 2 cut(s) 351, 405
BseJI GATNNNNATC 1 cut(s) 76
BseLI CCNNNNNNNGG 2 cut(s) 47, 400
BseNI ACTGG 1 cut(s) 557
BseRI GAGGAG 1 cut(s) 784
BseSI GKGCMC 1 cut(s) 504
BseXI GCAGC 1 cut(s) 485
Bsh1236I CGCG 2 cut(s) 279, 755
BshFI GGCC 2 cut(s) 607, 686
BshNI GGYRCC 1 cut(s) 37
BsiHKCI CYCGRG 1 cut(s) 394
BsiSI CCGG 2 cut(s) 234, 442
BslFI GGGAC 2 cut(s) 377, 651
BslI CCNNNNNNNGG 2 cut(s) 47, 400
BsmFI GGGAC 2 cut(s) 377, 651
BsnI GGCC 2 cut(s) 607, 686
BsoBI CYCGRG 1 cut(s) 394
Bsp119I TTCGAA 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 504
Bsp143I GATC 4 cut(s) 196, 280, 624, 657
Bsp19I CCATGG 1 cut(s) 687
BspACI CCGC 4 cut(s) 108, 277, 291, 755
BspANI GGCC 2 cut(s) 607, 686
BspFNI CGCG 2 cut(s) 279, 755
BspLI GGNNCC 2 cut(s) 39, 615
BspMAI CTGCAG 1 cut(s) 794
BspMI ACCTGC 1 cut(s) 25
BspPI GGATC 1 cut(s) 204
BspT104I TTCGAA 1 cut(s) 128
BspT107I GGYRCC 1 cut(s) 37
BsrFI RCCGGY 1 cut(s) 441
BsrI ACTGG 1 cut(s) 557
BssAI RCCGGY 1 cut(s) 441
BssECI CCNNGG 5 cut(s) 315, 355, 395, 687, 745
BssMI GATC 4 cut(s) 196, 280, 624, 657
BssT1I CCWWGG 1 cut(s) 687
Bst2UI CCWGG 2 cut(s) 317, 453
Bst4CI ACNGT 4 cut(s) 23, 302, 359, 579
Bst6I CTCTTC 1 cut(s) 72
BstBI TTCGAA 1 cut(s) 128
BstC8I GCNNGC 2 cut(s) 10, 443
BstDEI CTNAG 2 cut(s) 42, 641
BstDSI CCRYGG 2 cut(s) 355, 687
BstF5I GGATG 2 cut(s) 351, 405
BstFNI CGCG 2 cut(s) 279, 755
BstHHI GCGC 1 cut(s) 14
BstKTI GATC 4 cut(s) 199, 283, 627, 660
BstMBI GATC 4 cut(s) 196, 280, 624, 657
BstMWI GCNNNNNNNGC 1 cut(s) 482
BstNI CCWGG 2 cut(s) 317, 453
BstNSI RCATGY 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 315, 451
BstSFI CTRYAG 1 cut(s) 790
BstSLI GKGCMC 1 cut(s) 504
BstUI CGCG 2 cut(s) 279, 755
BstV1I GCAGC 1 cut(s) 485
BstV2I GAAGAC 1 cut(s) 97
BstX2I RGATCY 2 cut(s) 196, 624
BstYI RGATCY 2 cut(s) 196, 624
BsuRI GGCC 2 cut(s) 607, 686
BtgI CCRYGG 2 cut(s) 355, 687
BtrI CACGTC 1 cut(s) 499
BtsCI GGATG 2 cut(s) 351, 405
BtsIMutI CAGTG 1 cut(s) 575
BveI ACCTGC 1 cut(s) 25
Cac8I GCNNGC 2 cut(s) 10, 443
CaiI CAGNNNCTG 1 cut(s) 528
CfoI GCGC 1 cut(s) 14
Cfr10I RCCGGY 1 cut(s) 441
Cfr13I GGNCC 5 cut(s) 449, 605, 671, 684, 757
Csp6I GTAC 2 cut(s) 19, 38
CviAII CATG 6 cut(s) 16, 257, 373, 584, 688, 727
CviJI RGCY 7 cut(s) 441, 476, 525, 556, 607, 686, 782
CviKI_1 RGCY 7 cut(s) 441, 476, 525, 556, 607, 686, 782
CviQI GTAC 2 cut(s) 19, 38
DdeI CTNAG 2 cut(s) 42, 641
DpnI GATC 4 cut(s) 198, 282, 626, 659
DpnII GATC 4 cut(s) 196, 280, 624, 657
Eam1104I CTCTTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 72
Eco130I CCWWGG 1 cut(s) 687
Eco47I GGWCC 3 cut(s) 449, 671, 757
Eco57I CTGAAG 1 cut(s) 121
Eco88I CYCGRG 1 cut(s) 394
EcoO109I RGGNCCY 1 cut(s) 449
EcoRI GAATTC 1 cut(s) 518
EcoRII CCWGG 2 cut(s) 315, 451
EcoT14I CCWWGG 1 cut(s) 687
ErhI CCWWGG 1 cut(s) 687
FaeI CATG 6 cut(s) 19, 260, 376, 587, 691, 730
FaqI GGGAC 2 cut(s) 377, 651
FatI CATG 6 cut(s) 15, 256, 372, 583, 687, 726
Fnu4HI GCNGC 2 cut(s) 292, 474
FokI GGATG 2 cut(s) 338, 412
Fsp4HI GCNGC 2 cut(s) 292, 474
FspBI CTAG 3 cut(s) 218, 348, 663
GlaI GCGC 1 cut(s) 13
GluI GCNGC 2 cut(s) 292, 474
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 2 cut(s) 607, 686
HapII CCGG 2 cut(s) 234, 442
HhaI GCGC 1 cut(s) 14
Hin1II CATG 6 cut(s) 19, 260, 376, 587, 691, 730
Hin6I GCGC 1 cut(s) 12
HinP1I GCGC 1 cut(s) 12
HinfI GANTC 3 cut(s) 412, 418, 750
HpaII CCGG 2 cut(s) 234, 442
HphI GGTGA 4 cut(s) 51, 114, 331, 388
Hpy166II GTNNAC 2 cut(s) 322, 700
Hpy188I TCNGA 8 cut(s) 140, 157, 185, 388, 417, 437, 736, 749
Hpy188III TCNNGA 6 cut(s) 218, 394, 425, 548, 563, 632
Hpy8I GTNNAC 2 cut(s) 322, 700
Hpy99I CGWCG 1 cut(s) 500
HpyAV CCTTC 5 cut(s) 135, 145, 240, 514, 662
HpyCH4III ACNGT 4 cut(s) 23, 302, 359, 579
HpyCH4IV ACGT 2 cut(s) 334, 498
HpyCH4V TGCA 7 cut(s) 34, 243, 460, 473, 485, 766, 792
HpyF10VI GCNNNNNNNGC 1 cut(s) 482
HpyF3I CTNAG 2 cut(s) 42, 641
HpySE526I ACGT 2 cut(s) 334, 498
Hsp92II CATG 6 cut(s) 19, 260, 376, 587, 691, 730
HspAI GCGC 1 cut(s) 12
KpnI GGTACC 1 cut(s) 41
KroI GCCGGC 1 cut(s) 441
KroNI GCCGGC 1 cut(s) 443
Kzo9I GATC 4 cut(s) 196, 280, 624, 657
LmnI GCTCC 1 cut(s) 530
Lsp1109I GCAGC 1 cut(s) 485
LweI GCATC 3 cut(s) 86, 148, 360
MaeI CTAG 3 cut(s) 218, 348, 663
MaeII ACGT 2 cut(s) 334, 498
MaeIII GTNAC 3 cut(s) 57, 259, 376
MalI GATC 4 cut(s) 198, 282, 626, 659
MboI GATC 4 cut(s) 196, 280, 624, 657
MboII GAAGA 5 cut(s) 59, 99, 102, 275, 413
MflI RGATCY 2 cut(s) 196, 624
MhlI GDGCHC 1 cut(s) 504
MluCI AATT 4 cut(s) 343, 518, 540, 596
MlyI GAGTC 1 cut(s) 759
MnlI CCTC 9 cut(s) 336, 390, 555, 581, 627, 628, 749, 755, 762
MroNI GCCGGC 1 cut(s) 441
MroXI GAANNNNTTC 1 cut(s) 134
MseI TTAA 3 cut(s) 113, 509, 590
MslI CAYNNNNRTG 1 cut(s) 387
MspI CCGG 2 cut(s) 234, 442
MspR9I CCNGG 2 cut(s) 317, 453
MvaI CCWGG 2 cut(s) 317, 453
MvnI CGCG 2 cut(s) 279, 755
MwoI GCNNNNNNNGC 1 cut(s) 482
NaeI GCCGGC 1 cut(s) 443
NcoI CCATGG 1 cut(s) 687
NdeII GATC 4 cut(s) 196, 280, 624, 657
NgoMIV GCCGGC 1 cut(s) 441
NlaIII CATG 6 cut(s) 19, 260, 376, 587, 691, 730
NlaIV GGNNCC 2 cut(s) 39, 615
NmuCI GTSAC 2 cut(s) 57, 376
NspI RCATGY 1 cut(s) 260
NspV TTCGAA 1 cut(s) 128
PciI ACATGT 1 cut(s) 256
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PdiI GCCGGC 1 cut(s) 443
PdmI GAANNNNTTC 1 cut(s) 134
PfeI GAWTC 2 cut(s) 412, 418
PkrI GCNGC 2 cut(s) 293, 475
PleI GAGTC 1 cut(s) 758
PpsI GAGTC 1 cut(s) 758
PpuMI RGGWCCY 1 cut(s) 449
PscI ACATGT 1 cut(s) 256
Psp5II RGGWCCY 1 cut(s) 449
Psp6I CCWGG 2 cut(s) 315, 451
PspGI CCWGG 2 cut(s) 315, 451
PspN4I GGNNCC 2 cut(s) 39, 615
PspPI GGNCC 5 cut(s) 449, 605, 671, 684, 757
PspPPI RGGWCCY 1 cut(s) 449
PstI CTGCAG 1 cut(s) 794
PstNI CAGNNNCTG 1 cut(s) 528
PsuI RGATCY 2 cut(s) 196, 624
RsaI GTAC 2 cut(s) 20, 39
RsaNI GTAC 2 cut(s) 19, 38
RseI CAYNNNNRTG 1 cut(s) 387
SaqAI TTAA 3 cut(s) 113, 509, 590
SatI GCNGC 2 cut(s) 292, 474
Sau3AI GATC 4 cut(s) 196, 280, 624, 657
Sau96I GGNCC 5 cut(s) 449, 605, 671, 684, 757
SchI GAGTC 1 cut(s) 759
ScrFI CCNGG 2 cut(s) 317, 453
SduI GDGCHC 1 cut(s) 504
SfaNI GCATC 3 cut(s) 86, 148, 360
SfcI CTRYAG 1 cut(s) 790
SfuI TTCGAA 1 cut(s) 128
SinI GGWCC 3 cut(s) 449, 671, 757
SmiMI CAYNNNNRTG 1 cut(s) 387
SmlI CTYRAG 2 cut(s) 162, 632
SmoI CTYRAG 2 cut(s) 162, 632
Sse9I AATT 4 cut(s) 343, 518, 540, 596
SsiI CCGC 4 cut(s) 108, 277, 291, 755
SspI AATATT 1 cut(s) 210
SspMI CTAG 3 cut(s) 218, 348, 663
StyD4I CCNGG 2 cut(s) 315, 451
StyI CCWWGG 1 cut(s) 687
TaaI ACNGT 4 cut(s) 23, 302, 359, 579
TaiI ACGT 2 cut(s) 337, 501
TaqI TCGA 4 cut(s) 70, 128, 426, 495
TasI AATT 4 cut(s) 343, 518, 540, 596
TatI WGTACW 1 cut(s) 18
TauI GCSGC 1 cut(s) 294
TfiI GAWTC 2 cut(s) 412, 418
Tru1I TTAA 3 cut(s) 113, 509, 590
Tru9I TTAA 3 cut(s) 113, 509, 590
TscAI CASTG 1 cut(s) 582
TseFI GTSAC 2 cut(s) 57, 376
TseI GCWGC 1 cut(s) 473
Tsp45I GTSAC 2 cut(s) 57, 376
TspRI CASTG 1 cut(s) 582
VpaK11BI GGWCC 3 cut(s) 449, 671, 757
XapI RAATTY 3 cut(s) 343, 518, 540
XbaI TCTAGA 1 cut(s) 217
XceI RCATGY 1 cut(s) 260
XmnI GAANNNNTTC 1 cut(s) 134
XspI CTAG 3 cut(s) 218, 348, 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.