RLG00000025283

DNA polymerase III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003855
Physical Location & Seq
Forward (+)
43589948 .. 43591788
1841 bp
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UTR
Exon/CDS
Intron
RLM00000025283

Sequence Viewer

Length: 1014 bp
ATGAGAACCTTTTCTTTATCAAGAGTTCCCAGATGTGGACTTTATAACTTAGCTCAATTTTGGGGTGACAACTTCCACAGTTCGATTAGGACTTGTGGATACAGTTCCAGTTCTAAGCTGCATAGTTTCAAAATTTATGCGCTTGATGGAGGAAACAGTAGGAAATGGACTAGAAGGCCTCTAACAACAAATACAGAAGGAAGAAGCAAAACTGCCCAACACAGCAATCCCAGCAAGATTAGGCAGGAATTGTTGGATGTAACAGTTTCAACAAGTGCTACATTAAATATAAGTAAAATTGATGCAAGTCAATATGAAAAGATTCAACACCAGGACATTAGAGAATTGATAGCTGAGAATGAAGATTTAGCCAATCTAGTGACAGTTATTGTATTTGATATTGAGACTACTGGGTTTAGCAGAACAGATGATCGAATCATAGAGATTGCACTTCAAGATCTTCAAGGAGGTCAAAACAGTACTTTCCAGACACTGATAAACCCTGAACGTGATGTTCCATGGAATACAACCAAAATTCATGGAATTTCAACAGATATGGTCAACAAACCTGGTGTTCCAAGGATGAAGGAACTTTTACCAATCTTACTCAGCTATATCAAAAGCCGTCAAAAGCCGGGTGGTTATGTAATGTTGGTTGCTCACAATGGTCGTAGTTTTGATGTGCCCTTTCTCCGTAGTGAATTCTGTCGTTGCGGCGTAGAGTTTCCTTCAAACTGGCTGTTTGCAGACAGCCTTGTTATGGCACGGAAAGCAATGAAGTCTACAGGATCAGAAGCTTCTTCAAAATCATTAAAATTGCAAGACCTTCGTGCTCACCTTGGGATCCCATTGGTTGGTTCAGCTCACAGAGCCATGTCAGATGTGGTGGTGTTGTCAAAAGTGTTTCCAATATTAACTTATATTCTGAAACAGACACTTGCCAACGTCGTCGTTGAGCAATCTTTCTTACCCTCGGACCTCGACAATCCAAAGAAGAAGAAGAGTTCTGGGTAG

Protein Analysis

338

Amino Acids

37.68

Weight (kDa)

9.5

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 130 - 302 7e-24 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 45
AccB7I CCANNNNNTGG 1 cut(s) 854
AccI GTMKAC 1 cut(s) 782
AciI CCGC 1 cut(s) 714
AclWI GGATC 3 cut(s) 796, 838, 851
AcsI RAATTY 4 cut(s) 132, 534, 543, 701
AfaI GTAC 1 cut(s) 481
AfiI CCNNNNNNNGG 3 cut(s) 35, 760, 854
AgsI TTSAA 8 cut(s) 130, 270, 326, 455, 464, 549, 732, 804
AjnI CCWGG 2 cut(s) 330, 568
AluBI AGCT 6 cut(s) 53, 118, 353, 612, 797, 863
AluI AGCT 6 cut(s) 53, 118, 353, 612, 797, 863
Alw21I GWGCWC 1 cut(s) 835
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 3 cut(s) 796, 838, 851
AlwNI CAGNNNCTG 1 cut(s) 493
AoxI GGCC 1 cut(s) 176
ApeKI GCWGC 1 cut(s) 118
ApoI RAATTY 4 cut(s) 132, 534, 543, 701
Asp700I GAANNNNTTC 2 cut(s) 10, 321
AspLEI GCGC 1 cut(s) 142
AspS9I GGNCC 1 cut(s) 976
AsuC2I CCSGG 1 cut(s) 636
AsuHPI GGTGA 2 cut(s) 77, 827
AvaII GGWCC 1 cut(s) 976
BaeGI GKGCMC 1 cut(s) 687
BamHI GGATCC 1 cut(s) 843
Bbv12I GWGCWC 1 cut(s) 835
BbvI GCAGC 1 cut(s) 105
BccI CCATC 1 cut(s) 140
BceAI ACGGC 1 cut(s) 609
BcgI CGANNNNNNTGC 2 cut(s) 809, 843
BciT130I CCWGG 2 cut(s) 332, 570
BciVI GTATCC 1 cut(s) 92
BcnI CCSGG 1 cut(s) 636
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 2 cut(s) 171, 377
BfmI CTRYAG 1 cut(s) 783
BfuI GTATCC 1 cut(s) 92
BglII AGATCT 1 cut(s) 457
BisI GCNGC 2 cut(s) 119, 715
BlsI GCNGC 2 cut(s) 120, 716
BmcAI AGTACT 1 cut(s) 481
Bme1390I CCNGG 3 cut(s) 332, 570, 636
Bme18I GGWCC 1 cut(s) 976
BmgT120I GGNCC 1 cut(s) 976
BmiI GGNNCC 1 cut(s) 845
BmrFI CCNGG 3 cut(s) 332, 570, 636
BmrI ACTGGG 1 cut(s) 420
BmsI GCATC 1 cut(s) 292
BmuI ACTGGG 1 cut(s) 420
BpuMI CCSGG 1 cut(s) 636
BsaJI CCNNGG 4 cut(s) 518, 578, 838, 972
Bsc4I CCNNNNNNNGG 3 cut(s) 35, 760, 854
Bse1I ACTGG 3 cut(s) 108, 415, 740
Bse3DI GCAATG 1 cut(s) 780
BseBI CCWGG 2 cut(s) 332, 570
BseDI CCNNGG 4 cut(s) 518, 578, 838, 972
BseGI GGATG 2 cut(s) 262, 588
BseLI CCNNNNNNNGG 3 cut(s) 35, 760, 854
BseMI GCAATG 1 cut(s) 780
BseMII CTCAG 2 cut(s) 345, 622
BseNI ACTGG 3 cut(s) 108, 415, 740
BseSI GKGCMC 1 cut(s) 687
BseXI GCAGC 1 cut(s) 105
BseYI CCCAGC 1 cut(s) 230
BshFI GGCC 1 cut(s) 178
BsiHKAI GWGCWC 1 cut(s) 835
BsiSI CCGG 1 cut(s) 635
BslI CCNNNNNNNGG 3 cut(s) 35, 760, 854
BsmAI GTCTC 1 cut(s) 398
BsnI GGCC 1 cut(s) 178
Bsp1286I GDGCHC 2 cut(s) 687, 835
Bsp143I GATC 4 cut(s) 430, 457, 788, 843
Bsp19I CCATGG 1 cut(s) 518
BspACI CCGC 1 cut(s) 714
BspANI GGCC 1 cut(s) 178
BspCNI CTCAG 2 cut(s) 346, 621
BspLI GGNNCC 1 cut(s) 845
BspPI GGATC 3 cut(s) 796, 838, 851
BsrDI GCAATG 1 cut(s) 780
BsrI ACTGG 3 cut(s) 108, 415, 740
BssECI CCNNGG 4 cut(s) 518, 578, 838, 972
BssMI GATC 4 cut(s) 430, 457, 788, 843
BssT1I CCWWGG 3 cut(s) 518, 578, 838
Bst2UI CCWGG 2 cut(s) 332, 570
Bst4CI ACNGT 6 cut(s) 80, 104, 158, 265, 385, 479
Bst6I CTCTTC 1 cut(s) 995
BstDEI CTNAG 4 cut(s) 49, 114, 354, 608
BstDSI CCRYGG 1 cut(s) 518
BstF5I GGATG 2 cut(s) 262, 588
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 4 cut(s) 433, 460, 791, 846
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 4 cut(s) 430, 457, 788, 843
BstMWI GCNNNNNNNGC 3 cut(s) 231, 770, 869
BstNI CCWGG 2 cut(s) 332, 570
BstSCI CCNGG 3 cut(s) 330, 568, 634
BstSFI CTRYAG 1 cut(s) 783
BstSLI GKGCMC 1 cut(s) 687
BstV1I GCAGC 1 cut(s) 105
BstX2I RGATCY 2 cut(s) 457, 843
BstYI RGATCY 2 cut(s) 457, 843
BsuI GTATCC 1 cut(s) 92
BsuRI GGCC 1 cut(s) 178
BtgI CCRYGG 1 cut(s) 518
BtsCI GGATG 2 cut(s) 262, 588
BtsIMutI CAGTG 1 cut(s) 491
CaiI CAGNNNCTG 1 cut(s) 493
CfoI GCGC 1 cut(s) 142
Cfr13I GGNCC 1 cut(s) 976
CsiI ACCWGGT 1 cut(s) 568
Csp6I GTAC 1 cut(s) 480
CviAII CATG 3 cut(s) 519, 539, 874
CviQI GTAC 1 cut(s) 480
DdeI CTNAG 4 cut(s) 49, 114, 354, 608
DpnI GATC 4 cut(s) 432, 459, 790, 845
DpnII GATC 4 cut(s) 430, 457, 788, 843
Eam1104I CTCTTC 1 cut(s) 995
EarI CTCTTC 1 cut(s) 995
Eco130I CCWWGG 3 cut(s) 518, 578, 838
Eco147I AGGCCT 1 cut(s) 178
Eco47I GGWCC 1 cut(s) 976
EcoRI GAATTC 1 cut(s) 701
EcoRII CCWGG 2 cut(s) 330, 568
EcoT14I CCWWGG 3 cut(s) 518, 578, 838
ErhI CCWWGG 3 cut(s) 518, 578, 838
FaeI CATG 3 cut(s) 522, 542, 877
FatI CATG 3 cut(s) 518, 538, 873
FblI GTMKAC 1 cut(s) 782
Fnu4HI GCNGC 2 cut(s) 119, 715
FokI GGATG 2 cut(s) 269, 595
Fsp4HI GCNGC 2 cut(s) 119, 715
FspBI CTAG 2 cut(s) 171, 377
GlaI GCGC 1 cut(s) 141
GluI GCNGC 2 cut(s) 119, 715
GsaI CCCAGC 1 cut(s) 234
HaeIII GGCC 1 cut(s) 178
HapII CCGG 1 cut(s) 635
HhaI GCGC 1 cut(s) 142
Hin1II CATG 3 cut(s) 522, 542, 877
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HindIII AAGCTT 1 cut(s) 795
HinfI GANTC 2 cut(s) 322, 435
HpaII CCGG 1 cut(s) 635
HphI GGTGA 2 cut(s) 77, 827
Hpy166II GTNNAC 3 cut(s) 38, 562, 783
Hpy188I TCNGA 4 cut(s) 793, 880, 927, 976
Hpy188III TCNNGA 3 cut(s) 21, 455, 487
Hpy8I GTNNAC 3 cut(s) 38, 562, 783
Hpy99I CGWCG 2 cut(s) 950, 953
HpyAV CCTTC 5 cut(s) 168, 191, 580, 738, 836
HpyCH4III ACNGT 6 cut(s) 80, 104, 158, 265, 385, 479
HpyCH4IV ACGT 2 cut(s) 508, 945
HpyCH4V TGCA 5 cut(s) 121, 305, 449, 746, 820
HpyF10VI GCNNNNNNNGC 3 cut(s) 231, 770, 869
HpyF3I CTNAG 4 cut(s) 49, 114, 354, 608
HpySE526I ACGT 2 cut(s) 508, 945
Hsp92II CATG 3 cut(s) 522, 542, 877
HspAI GCGC 1 cut(s) 140
Kzo9I GATC 4 cut(s) 430, 457, 788, 843
Lsp1109I GCAGC 1 cut(s) 105
LweI GCATC 1 cut(s) 292
MabI ACCWGGT 1 cut(s) 568
MaeI CTAG 2 cut(s) 171, 377
MaeII ACGT 2 cut(s) 508, 945
MaeIII GTNAC 3 cut(s) 65, 259, 379
MalI GATC 4 cut(s) 432, 459, 790, 845
MboI GATC 4 cut(s) 430, 457, 788, 843
MboII GAAGA 7 cut(s) 213, 374, 452, 792, 1006, 1009, 1012
MflI RGATCY 2 cut(s) 457, 843
MhlI GDGCHC 2 cut(s) 687, 835
MluCI AATT 9 cut(s) 56, 132, 248, 297, 344, 534, 543, 701, 815
MmeI TCCRAC 1 cut(s) 234
MnlI CCTC 5 cut(s) 143, 189, 461, 982, 989
MroXI GAANNNNTTC 2 cut(s) 10, 321
MseI TTAA 3 cut(s) 284, 812, 914
MspI CCGG 1 cut(s) 635
MspR9I CCNGG 3 cut(s) 332, 570, 636
MvaI CCWGG 2 cut(s) 332, 570
MwoI GCNNNNNNNGC 3 cut(s) 231, 770, 869
NciI CCSGG 1 cut(s) 636
NcoI CCATGG 1 cut(s) 518
NdeII GATC 4 cut(s) 430, 457, 788, 843
NlaIII CATG 3 cut(s) 522, 542, 877
NlaIV GGNNCC 1 cut(s) 845
NmuCI GTSAC 2 cut(s) 65, 379
PceI AGGCCT 1 cut(s) 178
PdmI GAANNNNTTC 2 cut(s) 10, 321
PfeI GAWTC 2 cut(s) 322, 435
PflMI CCANNNNNTGG 1 cut(s) 854
PkrI GCNGC 2 cut(s) 120, 716
PsiI TTATAA 1 cut(s) 45
Psp6I CCWGG 2 cut(s) 330, 568
PspFI CCCAGC 1 cut(s) 230
PspGI CCWGG 2 cut(s) 330, 568
PspN4I GGNNCC 1 cut(s) 845
PspPI GGNCC 1 cut(s) 976
PstNI CAGNNNCTG 1 cut(s) 493
PsuI RGATCY 2 cut(s) 457, 843
RsaI GTAC 1 cut(s) 481
RsaNI GTAC 1 cut(s) 480
SaqAI TTAA 3 cut(s) 284, 812, 914
SatI GCNGC 2 cut(s) 119, 715
Sau3AI GATC 4 cut(s) 430, 457, 788, 843
Sau96I GGNCC 1 cut(s) 976
ScaI AGTACT 1 cut(s) 481
ScrFI CCNGG 3 cut(s) 332, 570, 636
SduI GDGCHC 2 cut(s) 687, 835
SexAI ACCWGGT 1 cut(s) 568
SfaNI GCATC 1 cut(s) 292
SfcI CTRYAG 1 cut(s) 783
SinI GGWCC 1 cut(s) 976
Sse9I AATT 9 cut(s) 56, 132, 248, 297, 344, 534, 543, 701, 815
SseBI AGGCCT 1 cut(s) 178
SsiI CCGC 1 cut(s) 714
SspI AATATT 1 cut(s) 912
SspMI CTAG 2 cut(s) 171, 377
StuI AGGCCT 1 cut(s) 178
StyD4I CCNGG 3 cut(s) 330, 568, 634
StyI CCWWGG 3 cut(s) 518, 578, 838
TaaI ACNGT 6 cut(s) 80, 104, 158, 265, 385, 479
TaiI ACGT 2 cut(s) 511, 948
TaqI TCGA 3 cut(s) 83, 433, 981
TasI AATT 9 cut(s) 56, 132, 248, 297, 344, 534, 543, 701, 815
TatI WGTACW 1 cut(s) 479
TauI GCSGC 1 cut(s) 717
TfiI GAWTC 2 cut(s) 322, 435
Tru1I TTAA 3 cut(s) 284, 812, 914
Tru9I TTAA 3 cut(s) 284, 812, 914
TscAI CASTG 1 cut(s) 498
TseFI GTSAC 2 cut(s) 65, 379
TseI GCWGC 1 cut(s) 118
Tsp45I GTSAC 2 cut(s) 65, 379
TspDTI ATGAA 5 cut(s) 330, 375, 527, 599, 791
TspGWI ACGGA 2 cut(s) 683, 781
TspRI CASTG 1 cut(s) 498
Van91I CCANNNNNTGG 1 cut(s) 854
VpaK11BI GGWCC 1 cut(s) 976
XapI RAATTY 4 cut(s) 132, 534, 543, 701
XcmI CCANNNNNNNNNTGG 1 cut(s) 880
XmiI GTMKAC 1 cut(s) 782
XmnI GAANNNNTTC 2 cut(s) 10, 321
XspI CTAG 2 cut(s) 171, 377
ZrmI AGTACT 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.