Prupe.1G126500_v2.0.a1

DNA polymerase III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0003855
Physical Location & Seq
Forward (+)
9956943 .. 9958581
1639 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G126500.2

Sequence Viewer

Length: 813 bp
ATGAGGACATGCTCCATGTGCTATTCGAACTTGCAAATACCAAAGTTGGGACTTCAGTCACCTTCTCTTGGATTAACATCTTTAGGCAGAAGAGCAACAATAACCACCATAAGTCAACCACTTTTAGAATGCTTCAGAAGCATACAATACTCTGATGCTCCAGCAAAGATTTTGGACAACCAATTTTTAAAGCATTTTGTGAAAGTATTTGGTCTGGATCTTGAAACCACTGGACTTTCCAGAAAGGAAGGACGGATTGTTGAAATTGCAGTCCGGGATCTTCATGGAGGCAAGAACAGTTGTTTCCAAACCCTGGTAAACCCCGAACAACATGTTCCAAATTCTCATATCCATGGTATAACAACCGACATGGTGACACATTCAGGTGTCCCAAGGATGGCGGAGTTGATTCCAATCCTTGTCGAATACATCAAAAGCCGCCAAGTACCCGGAGGGCATGTTATACTTGCAGCTCACAATGCACGCTGTTTCGATGTACCATTTTTGCTCAAGGAATTCAGCTGCTGCTCATTTGATGTTCCTCCTGACTGGTTGTTTCTTGACACCCTTGCTCTGGCTCGAGAGTTGAGGAAGTTACATGGATCAAAGGCTCTTCCAAAGATCTCACTTCAGGGACTTCGTGAGTTCTATAGGATCCCTCTAAAGGGTTCAAATCACAGGGCCATGTCAGATGTAAATGTGCTATCATCTATACTTCCCAACATGACTTTTGACCTGAAGCTTGGTGTCGATGACCTTTTGCAGAGGACTTTTAAGGCATCGGCCTGCAACATTACGCGAATAAGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T

Protein Analysis

271

Amino Acids

30.16

Weight (kDa)

9.26

Isoelectric Point (pI)

46.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 313
AccII CGCG 1 cut(s) 799
AciI CCGC 2 cut(s) 401, 439
AclWI GGATC 5 cut(s) 225, 285, 610, 649, 662
AcsI RAATTY 2 cut(s) 340, 515
AcuI CTGAAG 4 cut(s) 38, 118, 614, 758
AfaI GTAC 2 cut(s) 447, 498
AfiI CCNNNNNNNGG 7 cut(s) 47, 68, 313, 397, 574, 664, 665
AflIII ACRYGT 1 cut(s) 331
AgsI TTSAA 3 cut(s) 224, 263, 672
AjnI CCWGG 1 cut(s) 312
AluBI AGCT 3 cut(s) 473, 522, 742
AluI AGCT 3 cut(s) 473, 522, 742
AlwI GGATC 5 cut(s) 225, 285, 610, 649, 662
AlwNI CAGNNNCTG 1 cut(s) 525
Ama87I CYCGRG 1 cut(s) 579
AoxI GGCC 2 cut(s) 681, 783
ApeKI GCWGC 3 cut(s) 470, 522, 525
ApoI RAATTY 2 cut(s) 340, 515
AspS9I GGNCC 1 cut(s) 681
AsuC2I CCSGG 2 cut(s) 275, 450
AsuHPI GGTGA 2 cut(s) 51, 385
AsuII TTCGAA 1 cut(s) 26
AvaI CYCGRG 1 cut(s) 579
BamHI GGATCC 1 cut(s) 654
BbvI GCAGC 3 cut(s) 482, 509, 512
BccI CCATC 1 cut(s) 391
BciT130I CCWGG 1 cut(s) 314
BcnI CCSGG 2 cut(s) 275, 450
BfmI CTRYAG 1 cut(s) 649
BglII AGATCT 1 cut(s) 621
BisI GCNGC 4 cut(s) 439, 471, 523, 526
BlsI GCNGC 4 cut(s) 440, 472, 524, 527
Bme1390I CCNGG 3 cut(s) 275, 314, 450
BmeT110I CYCGRG 1 cut(s) 579
BmgT120I GGNCC 1 cut(s) 681
BmiI GGNNCC 1 cut(s) 656
BmrFI CCNGG 3 cut(s) 275, 314, 450
BmsI GCATC 2 cut(s) 145, 788
BoxI GACNNNNGTC 1 cut(s) 55
BpmI CTGGAG 1 cut(s) 144
Bpu14I TTCGAA 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 494
BpuMI CCSGG 2 cut(s) 275, 450
BsaBI GATNNNNATC 2 cut(s) 76, 413
BsaJI CCNNGG 3 cut(s) 312, 352, 392
BsaXI ACNNNNNCTCC 2 cut(s) 444, 474
Bsc4I CCNNNNNNNGG 7 cut(s) 47, 68, 313, 397, 574, 664, 665
Bse1I ACTGG 2 cut(s) 235, 554
Bse8I GATNNNNATC 2 cut(s) 76, 413
BseBI CCWGG 1 cut(s) 314
BseDI CCNNGG 3 cut(s) 312, 352, 392
BseGI GGATG 1 cut(s) 402
BseJI GATNNNNATC 2 cut(s) 76, 413
BseLI CCNNNNNNNGG 7 cut(s) 47, 68, 313, 397, 574, 664, 665
BseNI ACTGG 2 cut(s) 235, 554
BseXI GCAGC 3 cut(s) 482, 509, 512
Bsh1236I CGCG 1 cut(s) 799
BshFI GGCC 2 cut(s) 683, 785
BsiHKCI CYCGRG 1 cut(s) 579
BsiSI CCGG 2 cut(s) 274, 450
BslFI GGGAC 3 cut(s) 63, 374, 648
BslI CCNNNNNNNGG 7 cut(s) 47, 68, 313, 397, 574, 664, 665
BsmFI GGGAC 3 cut(s) 63, 374, 648
BsmI GAATGC 1 cut(s) 134
BsnI GGCC 2 cut(s) 683, 785
BsoBI CYCGRG 1 cut(s) 579
Bsp119I TTCGAA 1 cut(s) 26
Bsp143I GATC 5 cut(s) 217, 277, 602, 621, 654
Bsp19I CCATGG 1 cut(s) 352
BspACI CCGC 2 cut(s) 401, 439
BspANI GGCC 2 cut(s) 683, 785
BspFNI CGCG 1 cut(s) 799
BspLI GGNNCC 1 cut(s) 656
BspPI GGATC 5 cut(s) 225, 285, 610, 649, 662
BspQI GCTCTTC 2 cut(s) 85, 618
BspT104I TTCGAA 1 cut(s) 26
BsrI ACTGG 2 cut(s) 235, 554
BssECI CCNNGG 3 cut(s) 312, 352, 392
BssMI GATC 5 cut(s) 217, 277, 602, 621, 654
BssT1I CCWWGG 2 cut(s) 352, 392
Bst2UI CCWGG 1 cut(s) 314
Bst4CI ACNGT 1 cut(s) 299
Bst6I CTCTTC 2 cut(s) 85, 618
BstBI TTCGAA 1 cut(s) 26
BstC8I GCNNGC 2 cut(s) 484, 787
BstDSI CCRYGG 1 cut(s) 352
BstF5I GGATG 1 cut(s) 402
BstFNI CGCG 1 cut(s) 799
BstKTI GATC 5 cut(s) 220, 280, 605, 624, 657
BstMBI GATC 5 cut(s) 217, 277, 602, 621, 654
BstMWI GCNNNNNNNGC 3 cut(s) 18, 138, 479
BstNI CCWGG 1 cut(s) 314
BstNSI RCATGY 3 cut(s) 12, 335, 461
BstPAI GACNNNNGTC 1 cut(s) 55
BstSCI CCNGG 3 cut(s) 273, 312, 448
BstSFI CTRYAG 1 cut(s) 649
BstUI CGCG 1 cut(s) 799
BstV1I GCAGC 3 cut(s) 482, 509, 512
BstX2I RGATCY 4 cut(s) 217, 277, 621, 654
BstYI RGATCY 4 cut(s) 217, 277, 621, 654
BsuRI GGCC 2 cut(s) 683, 785
BtgI CCRYGG 1 cut(s) 352
BtsCI GGATG 1 cut(s) 402
BtsIMutI CAGTG 1 cut(s) 228
Cac8I GCNNGC 2 cut(s) 484, 787
CaiI CAGNNNCTG 1 cut(s) 525
Cfr13I GGNCC 1 cut(s) 681
Csp6I GTAC 2 cut(s) 446, 497
CviJI RGCY 8 cut(s) 438, 473, 522, 578, 611, 683, 742, 785
CviKI_1 RGCY 8 cut(s) 438, 473, 522, 578, 611, 683, 742, 785
CviQI GTAC 2 cut(s) 446, 497
DpnI GATC 5 cut(s) 219, 279, 604, 623, 656
DpnII GATC 5 cut(s) 217, 277, 602, 621, 654
DraI TTTAAA 1 cut(s) 189
Eam1104I CTCTTC 2 cut(s) 85, 618
EarI CTCTTC 2 cut(s) 85, 618
EciI GGCGGA 1 cut(s) 416
Eco130I CCWWGG 2 cut(s) 352, 392
Eco57I CTGAAG 4 cut(s) 38, 118, 614, 758
Eco88I CYCGRG 1 cut(s) 579
EcoRI GAATTC 1 cut(s) 515
EcoRII CCWGG 1 cut(s) 312
EcoT14I CCWWGG 2 cut(s) 352, 392
ErhI CCWWGG 2 cut(s) 352, 392
FaqI GGGAC 3 cut(s) 63, 374, 648
Fnu4HI GCNGC 4 cut(s) 439, 471, 523, 526
FokI GGATG 1 cut(s) 409
Fsp4HI GCNGC 4 cut(s) 439, 471, 523, 526
GluI GCNGC 4 cut(s) 439, 471, 523, 526
GsuI CTGGAG 1 cut(s) 144
HaeIII GGCC 2 cut(s) 683, 785
HapII CCGG 2 cut(s) 274, 450
HincII GTYRAC 1 cut(s) 116
HindII GTYRAC 1 cut(s) 116
HindIII AAGCTT 1 cut(s) 740
HinfI GANTC 1 cut(s) 409
HpaII CCGG 2 cut(s) 274, 450
HphI GGTGA 2 cut(s) 51, 385
Hpy166II GTNNAC 2 cut(s) 116, 319
Hpy188I TCNGA 3 cut(s) 137, 154, 691
Hpy188III TCNNGA 7 cut(s) 215, 221, 240, 545, 560, 581, 641
Hpy8I GTNNAC 2 cut(s) 116, 319
HpyAV CCTTC 2 cut(s) 72, 242
HpyCH4III ACNGT 1 cut(s) 299
HpyCH4V TGCA 6 cut(s) 34, 269, 470, 482, 763, 789
HpyF10VI GCNNNNNNNGC 3 cut(s) 18, 138, 479
Kzo9I GATC 5 cut(s) 217, 277, 602, 621, 654
LguI GCTCTTC 2 cut(s) 85, 618
LmnI GCTCC 2 cut(s) 17, 163
Lsp1109I GCAGC 3 cut(s) 482, 509, 512
LweI GCATC 2 cut(s) 145, 788
MaeIII GTNAC 3 cut(s) 57, 373, 594
MalI GATC 5 cut(s) 219, 279, 604, 623, 656
MboI GATC 5 cut(s) 217, 277, 602, 621, 654
MboII GAAGA 3 cut(s) 102, 272, 605
MflI RGATCY 4 cut(s) 217, 277, 621, 654
MluCI AATT 4 cut(s) 182, 264, 340, 515
MnlI CCTC 6 cut(s) 281, 446, 552, 582, 669, 759
MseI TTAA 3 cut(s) 74, 188, 774
MslI CAYNNNNRTG 2 cut(s) 351, 384
MspA1I CMGCKG 1 cut(s) 522
MspI CCGG 2 cut(s) 274, 450
MspR9I CCNGG 3 cut(s) 275, 314, 450
Mva1269I GAATGC 1 cut(s) 134
MvaI CCWGG 1 cut(s) 314
MvnI CGCG 1 cut(s) 799
MwoI GCNNNNNNNGC 3 cut(s) 18, 138, 479
NciI CCSGG 2 cut(s) 275, 450
NcoI CCATGG 1 cut(s) 352
NdeII GATC 5 cut(s) 217, 277, 602, 621, 654
NlaIV GGNNCC 1 cut(s) 656
NmuCI GTSAC 2 cut(s) 57, 373
NspI RCATGY 3 cut(s) 12, 335, 461
NspV TTCGAA 1 cut(s) 26
PaeR7I CTCGAG 1 cut(s) 579
PciI ACATGT 1 cut(s) 331
PciSI GCTCTTC 2 cut(s) 85, 618
PctI GAATGC 1 cut(s) 134
PfeI GAWTC 1 cut(s) 409
PflMI CCANNNNNTGG 1 cut(s) 313
PfoI TCCNGGA 1 cut(s) 273
PkrI GCNGC 4 cut(s) 440, 472, 524, 527
PscI ACATGT 1 cut(s) 331
PshAI GACNNNNGTC 1 cut(s) 55
Psp6I CCWGG 1 cut(s) 312
PspGI CCWGG 1 cut(s) 312
PspN4I GGNNCC 1 cut(s) 656
PspPI GGNCC 1 cut(s) 681
PstNI CAGNNNCTG 1 cut(s) 525
PsuI RGATCY 4 cut(s) 217, 277, 621, 654
PvuII CAGCTG 1 cut(s) 522
RsaI GTAC 2 cut(s) 447, 498
RsaNI GTAC 2 cut(s) 446, 497
RseI CAYNNNNRTG 2 cut(s) 351, 384
SapI GCTCTTC 2 cut(s) 85, 618
SaqAI TTAA 3 cut(s) 74, 188, 774
SatI GCNGC 4 cut(s) 439, 471, 523, 526
Sau3AI GATC 5 cut(s) 217, 277, 602, 621, 654
Sau96I GGNCC 1 cut(s) 681
ScrFI CCNGG 3 cut(s) 275, 314, 450
SetI ASST 7 cut(s) 64, 388, 475, 524, 738, 744, 759
SfaNI GCATC 2 cut(s) 145, 788
SfcI CTRYAG 1 cut(s) 649
Sfr274I CTCGAG 1 cut(s) 579
SfuI TTCGAA 1 cut(s) 26
SlaI CTCGAG 1 cut(s) 579
SmiMI CAYNNNNRTG 2 cut(s) 351, 384
SmlI CTYRAG 2 cut(s) 509, 579
SmoI CTYRAG 2 cut(s) 509, 579
Sse9I AATT 4 cut(s) 182, 264, 340, 515
SsiI CCGC 2 cut(s) 401, 439
StyD4I CCNGG 3 cut(s) 273, 312, 448
StyI CCWWGG 2 cut(s) 352, 392
TaaI ACNGT 1 cut(s) 299
TaqI TCGA 5 cut(s) 26, 423, 492, 580, 750
TasI AATT 4 cut(s) 182, 264, 340, 515
TauI GCSGC 1 cut(s) 441
TfiI GAWTC 1 cut(s) 409
Tru1I TTAA 3 cut(s) 74, 188, 774
Tru9I TTAA 3 cut(s) 74, 188, 774
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 2 cut(s) 57, 373
TseI GCWGC 3 cut(s) 470, 522, 525
Tsp45I GTSAC 2 cut(s) 57, 373
TspDTI ATGAA 1 cut(s) 272
TspGWI ACGGA 1 cut(s) 268
TspRI CASTG 1 cut(s) 235
Van91I CCANNNNNTGG 1 cut(s) 313
XapI RAATTY 2 cut(s) 340, 515
XceI RCATGY 3 cut(s) 12, 335, 461
XhoI CTCGAG 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.