Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0003855

Physical Location & Seq

Genomic Coordinates Forward (+)

1192076 .. 1213844

Gene Structure 21769 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0015100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 7866 bp

ATGGCGGCTCCGCCAGAATCTCTTCTCTCCGTCGCCGGGTCGGTCTCCACCGCCTCCACTCAGAAGCGGGTTCGGATCTTCCGGGATGAAATTCCGGCCATTATCGACGGCTCCGAAATATGTGTGGAGTCTGCAACACTGTTGGTAGATATAATATTTAAAACATTGTACATTTATGAGGATCGTGGGTCCAAGAAAGCTGTCGATGACATTATCACGAAATGTTTTCAAGAAGTTGCCTTCATGAAGAATTTTGCAGCAGCCCTTGTTCAGATGATGGAAAAAAATGTAAGGGTGCAATCACATGTTGGTGGCCACAGACTTTTCCAGTGGTCTTGTCTTCTTCTTAGCAAGAGTAAATTCGCCACAGTTTCCAAGAATGCATTCTGTAGAGTTGCCACAGTGCAGGCATCATTACTTCATATTGTTGTACAAACATCTCTCAGTGAGCAACGGGCTTGCAAGAAGACATTTTATCACTTACTTTCTCAGTCGCCGGACATCTACAAGTTGTATATAGAGGAGTTGAAGGAAGCACGCATCCCATACAAAGATAGTCCTGAATTATTATGGTTCTTGATGGAATTCTCAAGTACCTCAAGAAAATCATCTTCTTTGTTTGAACAATGCAAGCCAATATTTCTTGATATATATCTCAAGGCAATTTTAAACGCAAAAGAAAAGCCAGCAAAGGGTCTTAGCGAAGCTTTTCGTCCACTTTTTCGGCATATGTTGCATGAAGATTTTCAAAATGTTGTGCTTCCATCTTCTGTTAAAATGCTGAAACGCAACCCTGAGATTGTATTGGAATCAGTTGGCATTCTCTTGAAGTCAGTCAACCTTGATTTGAGCAAGTATGCTGTCGAAATCCTTTCACTGGTCTTGCCACAGGCTCGGCATGCAGATGAAGGCCGGAGGGTTGGTGCACTGGAAATCGTACGATGTCTGAGCCAAAATTCTAGTAATCCAGATGCTATAGAAGCAATGTTCAATGCTGTCAAATCTGTCCTTGGAGGCTCAGAGGGAAGACTTACATTTCCTTACCAGAGAATTGGCATGATCAATGCACTGCAAGAATTGTGTAATGCTCCAGATGGGAAGCACCTTAATAGACTGTCACAGACCTCATGCAATTACCTTCAATCTTGTTACAAGGAGGATGGCAATGAGGAGGTCAAGCTTGCAATTTTGTCAGCTCTAGGTTCGTGGGCAGCAAGATCTGCTGATGTTGTTCAGTCAGATTTAGTTTCTTTTTTGTCTTCTGGGCTTAAGGAGAAAGAAGCTCTAAGAAGAGGGCATCTTCGTTGTCTACGAGCCATATGCAGAAATACAGATGCTGTTTATCGGTTATCATCATTACTAGAACCTCTCATTCAACTTGTCAAAACTGGTTTTACTAAAGGAGTTCAGCGTTTGGATGGTATATATGCCTTGCTATTAGTTGGCAAAATTGCAGCAGTTGACATCAAAGCAGAGGAGACCGTGATGAAGGAAAAGATCTGGTCTGTGATATCTCAAAATGAGCCATCACTTCTTCCAATATCCTTGGCTTCAAAATTGTTTACTGAGGACTGCATGGCATGTGTCGATCTTCTTGAAGTCATGCTGGTGGAACATTTGCAGAGGGCGTTGGATTCCTTTTCTGTCAGATCACTGTCACAGTTGATAATATTTTTCATGTGCCACCCATGTTGGGATATTCGAAGAGTGGCTTATAATGCTACCAAAAAGATAGTTGCTGCTGCCCCACAATTAGCTGAACATCTTTTGATTGAATTCACGACTTTCATGTCTGTAGTTGAAGAGAAACATCGTATTTCAAAGTCAAGTGATACAGATAGTTCATTGGACTCCCAAGTACCGTTCCTTCCCTCGGTAGAGGTTTCAATAAAGGCTTTACTTGTTATATCATCAGCAGTTCTACCGGCAGCTCCAAGTGCATCAATGCGAGTTTTATTATGCGGACATCATCCATACTTAGTTGGAACTGCAAAAAGAGATGCAGTTTGGAGGAGGCTACATAAATGTTTGCATAAATGTGGTTTTGACATAAACAGCAATATTTTGGCTGACATGGTGAAATTGTGCAAGGGCCTACTGGAAACAATGTGGTTGTCCAGTACTACTCAATCAGAGCAGCAAGCAGCAATATCCTCATTAGCCACTCTGATGTCCATTGCACCTGGAGAAACGTATGTGGAGTTTGAAAAGCACTTGAAACACCTTCCTTACCGATATTCACATGATACGCTTTCCGAAAATGATATTCGGATTTTTCATACTCCTGAAGGCTTGCTTTCCAGTGAGCAAGGCGTCTACATAGCCGAGTCTGTTGCTGCCAAGAACATGAAACAAGCTAAGGGCCGTTTTCGGATGTATGAGGACCTAAATGACATGGATAATGGTGGTTCTAATCATTCTGCAAAAGTCGAGCCAAGCAGTAAAACTGGGAAGTCGACAAAGAAACCTGAGAAAACAAAGACTGCAAAAGAGGAGGCACGTGAGTTACAACTTAAGGAGGAGGCATCCATACGTGAGAAGGTCCGAGAAATACAGAAAAACCTGTCTTTAATGCTTAAAGCTCTTGGGGAAATGGCCATTGCAAATCCTGTTTTTGCACATAGTCGACTTCCTTCCCTGGTTAACTATGTCAATCCATTACTAAGGTCACCAATAGTCAGCGATATTGCATATGAAACTATGGTGAAACTCTCTAGATGTACTGCTCCCCCTCTTTGTAATTGGGGCCTTGACATTGCTACTGCTTTGCGCTTAGTTGTGACTGAAGAAGATCGTCTTCTGTTGGATATGATTTCATCGGTTGGGCAAGGGGAAGCCGATGATAGACCATCTTTGAGTCTATTTGAGCGAATTATTAACGCCATTTCTGCATCTTGTAAATCGGGACCTCTTCCTGTTGATTCCTTCACTTTTGTCTTCCCTATAATGGAGCGAATTCTTTTAAGCTCTAAGAAGACCGGGCTTCATGATAATGTGCTTCAGATTATTTACTTGCACATGGACCCCCTATTACCCCTCCCTAGGCTTCGAATGATTTCAGTTCTTTATCATGTTCTTGGTATTGTACAAGCTTATCAATCCTCTATTGGACCTGCGTTGAATGAGTTGTGTCTTGGTCTGCAGCCAGATGAAGTTGCACCGGCTTTATACGGGGTTTATGCAAAAGATGTTCATGTTAGAATGGCTTGTCTAACTGCTGTCAAATGCATTCCTACTGTTGCTAGTCGGTCTCTTATTCAGAATGTTGAGGTTGCTACAAGTATTTGGATTGCTTTACATGACCCAGAAAAGTCAGTTGCAGAAGCTGCAGAGGATTTGTGGGATCGCTATGGACATGACTTTGGGACTGACTATTCAGGCCTTTTCAAAGCACTTTCTCATATCCATTATACTGTCCGTTTTGCTGCTGCAGAGGCATTAGCTGCTGCTTTGGACGAAAATCCTGATTCTATACAGGAATCCCTTTCTACTTTATTCTCCCTGTATATCCGTGATGCTGGTTTGACTGAGAACAATGTCGATGCTGGTTGGCTGGGCCGGCAGGGTGTAGCTTTGGCATTGCATTCTTCTGCTGATGTACTAAGAACAAAAGACCTTCCTGTTGTTATGACCTTTTTGATTTCAAGAGCATTGGCAGATCCTAATGCTGATGTTCGGGGGAGAATGATTAATGCTGGCATTATGATTATTGATAAGCACGGAAGAGATAACGTGTCCTTGCTATTTCCCATCTTTGAGAACTACTTAAACAAAAAGGCATCGGATGAGGAGACATATGATTTGGTTCGTGAGGGTGTTGTTATTTTCACTGGAGCGTTGGCAAAGCATTTGGCAAAGGATGATCCAAAAGTACATGCTGTTATAGAGAAGTTGTTGGATGTGTTGAACACTCCATCAGAAGCTGTTCAGCGGGCGGTTTCAACATGCCTATCACCATTAATGCAATCAAAGCAAGATGATGGCCAAGCTCTTGTTTCAAGGATTTTGGATCAATTGATGAACAGTGATAAGTATGGGGAACGCCGTGGAGCTGCTTTTGGGCTTGCTGGAGTAGTCAAGGGATTTGGTATTTCTTCCTTGAAGAAGTATGGAATTGTGAATATACTACAGGAAGGACTTGTTGATAGGAATTCTGCAAAACGTCGTGAAGGGGGTTTGCTTGGATTTGAGTGTCTATGTGAAACACTTGGAAAATTATTTGAACCGTATGTAATACAAATGTTGCCACTACTGTTGGTTTCATTTTCTGATCAGGTTGTTGCAGTTCGTGAAGGTGCTGAGTGTGCTGCTCGGGCAATGATGTCTCACCTTACTGCTCAGGGAGTGAAACTGGTCCTTCCATCTCTTTTGAAGGGTCTTGAAGATAAAGCTTGGCGAACAAAGCAAAGTAGTGTTCAACTGCTTGGTGCTATGGCCTATTGTGCTCCTAAGCAGCTGTCTCAATGTCTCCCTAAGATTGTACCAAAACTGACTGAGGTTTTAACAGACACCCACCCTAAAGTTCAGTCAGCAGGACAAACAGCACTTCAACAGGTTGGGAGCGTAATAAAGAATCCTGAGATAGCTTCATTGGTCCCTACTCTTCTTCTGGGTCTTACAGATCCAAATGACTATACAAAATATTCTCTGGATGTTCTCTTGGGAACAACATTCATCAATTCAATTGATGCTCCTTCACTTGCACTCTTGGTGCCGATAGTACATAGAGGGCTAAGGGAGAGGGGTGCAGAGACTAAAAAGAAAGCAGCCCAAATAGTTGGCAATATGTGTTCGTTAGTTACAGATCCAAATGATATGATTCCGTACATAGGGCTGCTTCTTCCTGAAGTGAAAAAGGTTCTTGTGGATCCAATTCCAGAAGTTAGGTCAGTTGCTGCAAGGGCGCTTGGATCCCTCATAAGGGGAATGGGCGAAGATCATTTCCCAGATCTTGTTCCCTGGTTGTTGGATACACTTAAATCAGATAATAGTAATGTTGAGCGATCTGGAGCTGCTCAAGGGTTAAGTGAGGTATTAGCTGCACTTGGGACTGAGTACTTTGAGCATGTACTTCCTGATGTTATCCGAAATTGTTCTCATCAAAAAGCATCTGTGCGTGATGGGCATTTGACATTATTCAAGTATTTACCAAGGTCCTTAGGTGTCCAATTCCAGAATTATTTACAGCAGGTGCTGCCTGCTATTATAGATGGTCTTGCTGATGAAAATGAGTCTGTACGTGAAGCAGCATTAGGTGCGGGACATGTTCTGGTTGAGCATTATGCTATAACGTCGTTACCTTTGCTCCTACCCGCTGTTGAGGATGGTATCTTTAATGATAGTTGGCGAATTCGACAAAGCTCTGTGGAATTATTGGGGGATCTCTTATTTAAGGTCGCGGGTACTTCTGGAAAAGCTTTACTGGAGGGTGGCAGTGATGATGAAGGTGCAAGTACTGAAGCACAGGGGCGGGCTATCATTGAGGTTCTTGGAAGGGATAAGCGTGATGAAATTCTCGCTGCATTATATATGGTTCGAACTGATGTCAGCCTATCAGTGCGTCAGGCTGCATTGCATGTATGGAAAACTATAGTGGCAAATACCCCAAAAACTCTAAAGGAAATAATGCCAGTTCTAATGAATACTCTAATTACCTCTCTTGCATCATCATCCTTTGAAAGGCGGCAGGTCGCTGCACGTGCACTGGGTGAGCTTGTCAGGAAGCTTGGTGAAAGAGTGCTGCCATTGATCATCCCAATTTTATCCCAAGGATTGAAGGACGCTGACACCAGTAGACGACAAGGTGTATGCATTGGTCTGAGTGAAGTAATGGCATGTGCAGCTAAAAGTCATTTGTTGAGTTTCATGGATGAGCTGATTCCAACCATTCGAACTGCTCTTTCAGATAGCATGCCCGAAGTTCGTGAGTCTGCTGGAATAGCATTCAGCACTCTGTACAAGAATGCTGGAATGCAAGCGATTGATGAAATTGTTCCCAGTCTTTTGCATGCTTTGGAGGACGATCAAACTTCTGATACTGCACTTGATGGTCTCAAACAAATCTTAAGTGTCAGGATTACTGCGGTTTTACCCCACATTTTGCCCAAGCTTGTCCAGCTTCCACTAACTGCATTTAATGCACATGCTCTTGGAGCTGTAGCTGAGGTTGCTGGGCCTGGTCTTAACACTCATCTTGGTACCGTTCTTCCTGCTTTACTTACTTCAATGGGTGTTGATGCAAAGGATGTTCAAACATTGGCAAAGGAGGCTGCTGAAACTGTGGTCTTAGTTATAGATGAGGAAGGTGTTGAATATCTTACATCTGAGCTACTTAGAGCTGTCAGCGATAGTCAGGCTTCAATTAGAAGAAGTGCCACGTATCTTATAGGCTATTTCTTCAAAAACAGTAAATTATATTTGGTTGATGAAGCTCCAAACATGATATCTACCTTGATTGTCTTGCTCAGTGATTCTGATTCGGCTACTGTGGCGGTATCTTGGGAAGCATTGTCAAGGGTTGTAAGTTCAGTCCCCAAGGAGGTACTTCCTTCATATATTAAATTAGTACGAGATGCTGTGTCGACTTCCAGAGATAAGGAGCGTAGGAAAAAGAAGGGAGGACCTATTGTCATACCTGGATTTTGTTTACCAAAAGCACTTCAACCGTTGCTTCCAATATTTCTCCAGGGTTTAATAAGTGGATCTGCTGAACTGAGAGAGCAAGCGGCACTGGGTCTTGGAGAACTGATTGAAGTGACTAGTGAAAAGGCACTCAAAGAGTTTGTCATTCCAATTACAGGGCCTCTTATCCGGATAATAGGTGACCGGTTCCCTTGGCAGGTGAAGAGTGCTATTTTATCTACCTTGACAATCATAATCAGAAAAGGTGGGATGGCCTTGAAACCATTTCTTCCGCAGCTTCAAACAACATTTGTCAAGTGTCTACAGGATAACACCAGAATTGTTCGCTCAAGTGCTGCCCTTGCTCTCGGAAAGCTTAGTGCTTTAAGTACCCGGGTGGACCCTCTAGTTGGAGACCTCCTGTCCAGTCTGCAGGCTTTGGATAGTGGGGTTCGGGAGGCAAGTTTGTCTGCTTTAGAAGGTGTCCTAAAGTATGCTGGAAAGAGCGTGAGCAGTGCTGTTAGAACTCGTGTCTACTTGCAGCTTAAGGATTTGATTCATCATGATGATGATGAGGTTCGAGCATCTGCTGCAAGCATTTTGGGTATCATGTCCCAGTACATTGAAGATACCCAGCTGACCGAGCTGCTTCAGGAACTATCAAGTCTTCCATCGTCGCTCGGCTGGACTGCTAGGCATGGTTCTGTACTTACAATTTCATCCATGCTCAGGCACATCCCATCCACCATCTGTACTTCTCCAGTGTTTCCGTCTATCCTGGACCAGCTTAAAGCTGCCTTGACTGATGAGAAGTTCCCTCTTCGTGAAACTTCAACCAAGGCATTTGGGAGACTTCTTGTTTATAAAGTCCGAAGTGATCCTTCTAATATCTCAGTACAATTAGATATTATATCATCTTTAGTCTCAGCATTGCATGATGATTCTAGCGAAGTTAGAAGAAAGGCGTTATCTGCTCTAAAGGCAGTTTCGAAGGAAAGTTCTTCACCTATTGCGGCCCATATCAATATTATTGGACCTGCACTGGCTGAATGTTTGAAGGATGGAAGTACACCCGTGAGACTTGCAGCAGAAAGATGTGCTCTGCATGCCTTCCAATTGACAAAGGGTCCTGAAAATGTTCAAGCTGCACAAAAGTTCATCACAGGCTTGGAAGCTAGACGAATATCAAAGTTTTCAGAACATAGTGATGATAGTGAAGGTGAGGATGACTTGGCAACTGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000738 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006308 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009536 GO:0009987 GO:0016787 GO:0016788 GO:0019439 GO:0034641 GO:0034655 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0071704 GO:0090304 GO:0090305 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RNase_T

Protein Analysis

2621

Amino Acids

286.76

Weight (kDa)

6.61

Isoelectric Point (pI)

42.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GNC1_N	PF24993	211 - 344	2.3e-45	Stalled ribosome sensor GCN1 N-terminal
HEAT_EF3_N	PF24987	1348 - 1515	1.4e-46	EF3 N-terminal HEAT repeat
HEAT_GCN1	PF23271	1398 - 1535	2.7e-10	Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_GNC1	PF24984	1553 - 1639	5.8e-26	Elongation factor 3/GNC1 HEAT repeat
HEAT_MROH2B_C	PF23227	1616 - 1750	1e-05	MROH2B-like, HEAT repeats
HEAT_GCN1	PF23271	1627 - 1774	2e-11	Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_N	PF24987	1668 - 1797	2.7e-08	EF3 N-terminal HEAT repeat
TPR_DNAAF5	PF25757	1836 - 1992	2.6e-06	Dynein axonemal assembly factor 5-like, TPR repeats
HEAT_GCN1	PF23271	1870 - 2015	3.4e-06	Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_N	PF24987	1885 - 1978	8.1e-07	EF3 N-terminal HEAT repeat
HEAT_EF3_GNC1	PF24984	1906 - 1985	5e-06	Elongation factor 3/GNC1 HEAT repeat
HEAT_GCN1	PF23271	2029 - 2218	1.3e-51	Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_MROH2B_C	PF23227	2312 - 2426	1.6e-07	MROH2B-like, HEAT repeats
HEAT_GCN1_C_2	PF25801	2341 - 2592	2.7e-31	Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_GCN1_C	PF25786	2439 - 2593	3.2e-74	GCN1-like, C-terminal HEAT repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003855)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26940 AT5G26940 AT5G26940 AT5G26940
fragaria_vesca	FvH4_4g19220 FvH4_6g07110 FvH4_6g07110
malus_domestica	MD12G1208900.v1.1 MD12G1209000.v1.1 MD13G1159900.v1.1
prunus_persica	Prupe.1G126500_v2.0.a1 Prupe.1G126500_v2.0.a1 Prupe.6G316100_v2.0.a1 Prupe.6G316100_v2.0.a1 Prupe.6G316100_v2.0.a1
pyrus_communis	pycom12g19620 pycom13g13620
rosa_chinensis	RchiOBHm_Chr3g0456691 RchiOBHm_Chr4g0424201
rosa_laevigata	RLG00000007497 RLG00000025283
rosa_roxburghii	Rroxscaffold_11G00450390 Rroxscaffold_6G00422530
rosa_rugosa	Rorug03G0015100 Rorug04G0193700
rosa_samantha	Rh3AG075600 Rh3BG077500 Rh3CG077300 Rh3DG079200 Rh4AG251500 Rh4CG267500 Rh4DG251300
rosa_wichuraiana	Rw3G006480 Rw4G021690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1716, 7485
AarI	CACCTGC	2 cut(s)	5179, 6829
AasI	GACNNNNNNGTC	1 cut(s)	1789
Acc36I	ACCTGC	5 cut(s)	3121, 5179, 5677, 6829, 7666
Acc65I	GGTACC	1 cut(s)	6197
AccB1I	GGYRCC	2 cut(s)	4711, 6197
AccI	GTMKAC	8 cut(s)	1309, 2316, 2456, 2626, 5794, 6581, 6943, 7155
AccII	CGCG	1 cut(s)	5399
AccIII	TCCGGA	1 cut(s)	6810
AcoI	YGGCCR	4 cut(s)	96, 313, 2595, 3982
AcuI	CTGAAG	6 cut(s)	2307, 2805, 2984, 4866, 5478, 7256
AcvI	CACGTG	2 cut(s)	2501, 5699
AcyI	GRCGYC	1 cut(s)	2313
AdeI	CACNNNGTG	1 cut(s)	5708
AflII	CTTAAG	4 cut(s)	1268, 2513, 6064, 7166
AflIII	ACRYGT	3 cut(s)	304, 3732, 5263
AgeI	ACCGGT	1 cut(s)	6825
AhdI	GACNNNNNGTC	3 cut(s)	6626, 7042, 7746
AhlI	ACTAGT	1 cut(s)	6758
AjnI	CCWGG	7 cut(s)	2182, 2637, 4958, 6175, 6634, 6684, 7398
AjuI	GAANNNNNNNTTGG	2 cut(s)	368, 400
Alw21I	GWGCWC	4 cut(s)	928, 4447, 5704, 7723
Alw44I	GTGCAC	2 cut(s)	924, 5700
AlwNI	CAGNNNCTG	5 cut(s)	1337, 3326, 3923, 4895, 5194
Ama87I	CYCGRG	2 cut(s)	4311, 7014
Aor13HI	TCCGGA	1 cut(s)	6810
ApaLI	GTGCAC	2 cut(s)	924, 5700
ArsI	GACNNNNNNTTYG	4 cut(s)	3784, 3816, 6768, 6800
AseI	ATTAAT	2 cut(s)	3690, 3959
AsiGI	ACCGGT	1 cut(s)	6825
Asp700I	GAANNNNTTC	9 cut(s)	21, 383, 708, 744, 3055, 3924, 5092, 5991, 7758
Asp718I	GGTACC	1 cut(s)	6197
AspA2I	CCTAGG	1 cut(s)	3041
AspLEI	GCGC	2 cut(s)	2772, 4906
AsuC2I	CCSGG	5 cut(s)	37, 83, 2980, 7015, 7016
AsuII	TTCGAA	5 cut(s)	1702, 3049, 5536, 5890, 7610
AvaI	CYCGRG	2 cut(s)	4311, 7014
AvrII	CCTAGG	1 cut(s)	3041
AxyI	CCTNAGG	1 cut(s)	5158
BaeGI	GKGCMC	2 cut(s)	928, 5704
BalI	TGGCCA	3 cut(s)	315, 2597, 3984
BamHI	GGATCC	2 cut(s)	4867, 4910
BanI	GGYRCC	2 cut(s)	4711, 6197
BarI	GAAGNNNNNNTAC	2 cut(s)	1851, 1883
BauI	CACGAG	1 cut(s)	7149
BbrPI	CACGTG	2 cut(s)	2501, 5699
BbsI	GAAGAC	8 cut(s)	332, 473, 1033, 1251, 2789, 2929, 2981, 7280
Bbv12I	GWGCWC	4 cut(s)	928, 4447, 5704, 7723
BbvCI	CCTCAGC	1 cut(s)	6162
BceAI	ACGGC	3 cut(s)	124, 2349, 4029
BcgI	CGANNNNNNTGC	4 cut(s)	1302, 1336, 2315, 2349
BciT130I	CCWGG	7 cut(s)	2184, 2639, 4960, 6177, 6636, 6686, 7400
BciVI	GTATCC	1 cut(s)	4963
BclI	TGATCA	3 cut(s)	1059, 4270, 5748
BcnI	CCSGG	5 cut(s)	37, 83, 2980, 7015, 7016
BcuI	ACTAGT	1 cut(s)	6758
BfoI	RGCGCY	1 cut(s)	4907
BfrI	CTTAAG	4 cut(s)	1268, 2513, 6064, 7166
BfuAI	ACCTGC	5 cut(s)	3121, 5179, 5677, 6829, 7666
BfuI	GTATCC	1 cut(s)	4963
BglII	AGATCT	3 cut(s)	1217, 1497, 4948
BlnI	CCTAGG	1 cut(s)	3041
BmcAI	AGTACT	3 cut(s)	2122, 5057, 5455
BmeRI	GACNNNNNGTC	3 cut(s)	6626, 7042, 7746
BmeT110I	CYCGRG	2 cut(s)	4311, 7014
BmrI	ACTGGG	5 cut(s)	2457, 5714, 5991, 6740, 7231
BmuI	ACTGGG	5 cut(s)	2457, 5714, 5991, 6740, 7231
BpiI	GAAGAC	8 cut(s)	332, 473, 1033, 1251, 2789, 2929, 2981, 7280
BpmI	CTGGAG	8 cut(s)	1074, 2205, 3852, 4089, 5028, 5444, 6668, 7365
Bpu10I	CCTNAGC	6 cut(s)	2358, 4338, 4449, 4733, 6162, 7349
Bpu14I	TTCGAA	5 cut(s)	1702, 3049, 5536, 5890, 7610
BpuEI	CTTGAG	5 cut(s)	574, 583, 641, 5001, 6955
BpuMI	CCSGG	5 cut(s)	37, 83, 2980, 7015, 7016
BsaAI	YACGTR	5 cut(s)	2501, 2534, 5240, 5699, 6378
BsaBI	GATNNNNATC	1 cut(s)	651
BsaHI	GRCGYC	1 cut(s)	2313
BsaI	GGTCTC	5 cut(s)	49, 1472, 3255, 6056, 7029
BsaWI	WCCGGW	2 cut(s)	6810, 6825
BsaXI	ACNNNNNCTCC	4 cut(s)	907, 937, 5289, 5319
Bse118I	RCCGGY	4 cut(s)	1924, 3160, 3558, 6825
Bse21I	CCTNAGG	1 cut(s)	5158
Bse3DI	GCAATG	9 cut(s)	990, 1171, 2175, 2598, 2754, 3578, 4323, 5570, 7550
Bse8I	GATNNNNATC	1 cut(s)	651
BseAI	TCCGGA	1 cut(s)	6810
BseBI	CCWGG	7 cut(s)	2184, 2639, 4960, 6177, 6636, 6686, 7400
BseJI	GATNNNNATC	1 cut(s)	651
BseMI	GCAATG	9 cut(s)	990, 1171, 2175, 2598, 2754, 3578, 4323, 5570, 7550
BseRI	GAGGAG	7 cut(s)	536, 1184, 1490, 2026, 2507, 2534, 3803
BseSI	GKGCMC	2 cut(s)	928, 5704
BseYI	CCCAGC	3 cut(s)	3553, 6170, 7254
BsgI	GTGCAG	8 cut(s)	425, 4767, 5025, 5679, 5859, 6024, 7644, 7752
Bsh1236I	CGCG	1 cut(s)	5399
BshNI	GGYRCC	2 cut(s)	4711, 6197
BshTI	ACCGGT	1 cut(s)	6825
BsiHKAI	GWGCWC	4 cut(s)	928, 4447, 5704, 7723
BsiHKCI	CYCGRG	2 cut(s)	4311, 7014
BsiWI	CGTACG	1 cut(s)	937
BslFI	GGGAC	7 cut(s)	2919, 3379, 4580, 5062, 5274, 6515, 7219
BsmFI	GGGAC	7 cut(s)	2919, 3379, 4580, 5062, 5274, 6515, 7219
BsmI	GAATGC	8 cut(s)	383, 385, 819, 3228, 3583, 5942, 5968, 5976
Bso31I	GGTCTC	5 cut(s)	49, 1472, 3255, 6056, 7029
BsoBI	CYCGRG	2 cut(s)	4311, 7014
Bsp119I	TTCGAA	5 cut(s)	1702, 3049, 5536, 5890, 7610
Bsp1286I	GDGCHC	4 cut(s)	928, 4447, 5704, 7723
Bsp13I	TCCGGA	1 cut(s)	6810
Bsp1407I	TGTACA	4 cut(s)	168, 430, 3085, 5955
BspEI	TCCGGA	1 cut(s)	6810
BspFNI	CGCG	1 cut(s)	5399
BspHI	TCATGA	3 cut(s)	243, 2986, 7183
BspMAI	CTGCAG	4 cut(s)	3144, 3331, 3433, 7056
BspMI	ACCTGC	5 cut(s)	3121, 5179, 5677, 6829, 7666
BspT104I	TTCGAA	5 cut(s)	1702, 3049, 5536, 5890, 7610
BspT107I	GGYRCC	2 cut(s)	4711, 6197
BspTI	CTTAAG	4 cut(s)	1268, 2513, 6064, 7166
BspTNI	GGTCTC	5 cut(s)	49, 1472, 3255, 6056, 7029
BsrDI	GCAATG	9 cut(s)	990, 1171, 2175, 2598, 2754, 3578, 4323, 5570, 7550
BsrFI	RCCGGY	4 cut(s)	1924, 3160, 3558, 6825
BsrGI	TGTACA	4 cut(s)	168, 430, 3085, 5955
BssAI	RCCGGY	4 cut(s)	1924, 3160, 3558, 6825
BssNI	GRCGYC	1 cut(s)	2313
BssSI	CACGAG	1 cut(s)	7149
BssT1I	CCWWGG	8 cut(s)	1009, 1545, 3041, 5150, 5767, 6534, 6833, 7458
Bst2BI	CACGAG	1 cut(s)	7149
Bst2UI	CCWGG	7 cut(s)	2184, 2639, 4960, 6177, 6636, 6686, 7400
Bst6I	CTCTTC	9 cut(s)	27, 1285, 1699, 1797, 2916, 3718, 4608, 6839, 7446
BstACI	GRCGYC	1 cut(s)	2313
BstAFI	CTTAAG	4 cut(s)	1268, 2513, 6064, 7166
BstAPI	GCANNNNNTGC	8 cut(s)	733, 1220, 3326, 3443, 5194, 5255, 6137, 7211
BstAUI	TGTACA	4 cut(s)	168, 430, 3085, 5955
BstBAI	YACGTR	5 cut(s)	2501, 2534, 5240, 5699, 6378
BstBI	TTCGAA	5 cut(s)	1702, 3049, 5536, 5890, 7610
BstDSI	CCRYGG	1 cut(s)	4045
BstEII	GGTNACC	2 cut(s)	2667, 6821
BstENI	CCTNNNNNAGG	1 cut(s)	5677
BstFNI	CGCG	1 cut(s)	5399
BstH2I	RGCGCY	1 cut(s)	4907
BstHHI	GCGC	2 cut(s)	2772, 4906
BstNI	CCWGG	7 cut(s)	2184, 2639, 4960, 6177, 6636, 6686, 7400
BstPI	GGTNACC	2 cut(s)	2667, 6821
BstSLI	GKGCMC	2 cut(s)	928, 5704
BstUI	CGCG	1 cut(s)	5399
BstV2I	GAAGAC	8 cut(s)	332, 473, 1033, 1251, 2789, 2929, 2981, 7280
BstXI	CCANNNNNNTGG	2 cut(s)	1052, 4778
Bsu36I	CCTNAGG	1 cut(s)	5158
BsuI	GTATCC	1 cut(s)	4963
BtgI	CCRYGG	1 cut(s)	4045
BtsI	GCAGTG	3 cut(s)	1067, 5440, 7141
BveI	ACCTGC	5 cut(s)	3121, 5179, 5677, 6829, 7666
CaiI	CAGNNNCTG	5 cut(s)	1337, 3326, 3923, 4895, 5194
CciI	TCATGA	3 cut(s)	243, 2986, 7183
CfoI	GCGC	2 cut(s)	2772, 4906
Cfr10I	RCCGGY	4 cut(s)	1924, 3160, 3558, 6825
Cfr9I	CCCGGG	1 cut(s)	7014
CseI	GACGC	3 cut(s)	2302, 5549, 5789
CspAI	ACCGGT	1 cut(s)	6825
CspCI	CAANNNNNGTGG	4 cut(s)	1673, 1708, 4236, 4271
DraI	TTTAAA	2 cut(s)	160, 669
DraIII	CACNNNGTG	1 cut(s)	5708
DrdI	GACNNNNNNGTC	1 cut(s)	1789
DriI	GACNNNNNGTC	3 cut(s)	6626, 7042, 7746
DseDI	GACNNNNNNGTC	1 cut(s)	1789
EaeI	YGGCCR	4 cut(s)	96, 313, 2595, 3982
Eam1104I	CTCTTC	9 cut(s)	27, 1285, 1699, 1797, 2916, 3718, 4608, 6839, 7446
Eam1105I	GACNNNNNGTC	3 cut(s)	6626, 7042, 7746
EarI	CTCTTC	9 cut(s)	27, 1285, 1699, 1797, 2916, 3718, 4608, 6839, 7446
Eco130I	CCWWGG	8 cut(s)	1009, 1545, 3041, 5150, 5767, 6534, 6833, 7458
Eco147I	AGGCCT	1 cut(s)	3381
Eco31I	GGTCTC	5 cut(s)	49, 1472, 3255, 6056, 7029
Eco32I	GATATC	2 cut(s)	1512, 6444
Eco57I	CTGAAG	6 cut(s)	2307, 2805, 2984, 4866, 5478, 7256
Eco72I	CACGTG	2 cut(s)	2501, 5699
Eco81I	CCTNAGG	1 cut(s)	5158
Eco88I	CYCGRG	2 cut(s)	4311, 7014
Eco91I	GGTNACC	2 cut(s)	2667, 6821
EcoNI	CCTNNNNNAGG	1 cut(s)	5677
EcoO109I	RGGNCCY	8 cut(s)	2092, 2383, 2746, 2906, 5154, 6620, 6800, 7748
EcoO65I	GGTNACC	2 cut(s)	2667, 6821
EcoRI	GAATTC	5 cut(s)	584, 1775, 2955, 4150, 5349
EcoRII	CCWGG	7 cut(s)	2182, 2637, 4958, 6175, 6634, 6684, 7398
EcoRV	GATATC	2 cut(s)	1512, 6444
EcoT14I	CCWWGG	8 cut(s)	1009, 1545, 3041, 5150, 5767, 6534, 6833, 7458
EcoT22I	ATGCAT	3 cut(s)	385, 3230, 5813
ErhI	CCWWGG	8 cut(s)	1009, 1545, 3041, 5150, 5767, 6534, 6833, 7458
FalI	AAGNNNNNCTT	8 cut(s)	1696, 1728, 2280, 2312, 2781, 2813, 7486, 7518
FaqI	GGGAC	7 cut(s)	2919, 3379, 4580, 5062, 5274, 6515, 7219
FauI	CCCGC	6 cut(s)	60, 3924, 5251, 5320, 5392, 5463
FauNDI	CATATG	4 cut(s)	729, 1319, 2692, 3796
FbaI	TGATCA	3 cut(s)	1059, 4270, 5748
FblI	GTMKAC	8 cut(s)	1309, 2316, 2456, 2626, 5794, 6581, 6943, 7155
GlaI	GCGC	2 cut(s)	2771, 4905
GsaI	CCCAGC	3 cut(s)	3557, 6174, 7258
GsuI	CTGGAG	8 cut(s)	1074, 2205, 3852, 4089, 5028, 5444, 6668, 7365
HaeII	RGCGCY	1 cut(s)	4907
HgaI	GACGC	3 cut(s)	2302, 5549, 5789
HhaI	GCGC	2 cut(s)	2772, 4906
Hin1I	GRCGYC	1 cut(s)	2313
Hin6I	GCGC	2 cut(s)	2770, 4904
HinP1I	GCGC	2 cut(s)	2770, 4904
HincII	GTYRAC	6 cut(s)	838, 1462, 2457, 2627, 2644, 6582
HindII	GTYRAC	6 cut(s)	838, 1462, 2457, 2627, 2644, 6582
HindIII	AAGCTT	8 cut(s)	705, 1178, 3090, 4389, 5415, 5723, 6107, 6995
HpaI	GTTAAC	1 cut(s)	2644
Hpy99I	CGWCG	5 cut(s)	35, 110, 4167, 5296, 7300
HpyCH4IV	ACGT	9 cut(s)	2192, 2500, 2533, 3732, 4162, 5239, 5291, 5698, 6377
HpySE526I	ACGT	9 cut(s)	2192, 2500, 2533, 3732, 4162, 5239, 5291, 5698, 6377
Hsp92I	GRCGYC	1 cut(s)	2313
HspAI	GCGC	2 cut(s)	2770, 4904
Kpn2I	TCCGGA	1 cut(s)	6810
KpnI	GGTACC	1 cut(s)	6201
KroI	GCCGGC	1 cut(s)	3558
KroNI	GCCGGC	1 cut(s)	3560
Ksp22I	TGATCA	3 cut(s)	1059, 4270, 5748
KspAI	GTTAAC	1 cut(s)	2644
MaeII	ACGT	9 cut(s)	2192, 2500, 2533, 3732, 4162, 5239, 5291, 5698, 6377
MfeI	CAATTG	3 cut(s)	4013, 4683, 7736
MhlI	GDGCHC	4 cut(s)	928, 4447, 5704, 7723
MlsI	TGGCCA	3 cut(s)	315, 2597, 3984
MluNI	TGGCCA	3 cut(s)	315, 2597, 3984
MlyI	GAGTC	6 cut(s)	137, 1844, 2336, 2866, 5240, 5936
MmeI	TCCRAC	7 cut(s)	1611, 1963, 2784, 3876, 4947, 5906, 7012
Mox20I	TGGCCA	3 cut(s)	315, 2597, 3984
Mph1103I	ATGCAT	3 cut(s)	385, 3230, 5813
MroI	TCCGGA	1 cut(s)	6810
MroNI	GCCGGC	1 cut(s)	3558
MroXI	GAANNNNTTC	9 cut(s)	21, 383, 708, 744, 3055, 3924, 5092, 5991, 7758
MscI	TGGCCA	3 cut(s)	315, 2597, 3984
MslI	CAYNNNNRTG	9 cut(s)	309, 903, 1607, 2037, 2168, 2991, 7185, 7188, 7827
Msp20I	TGGCCA	3 cut(s)	315, 2597, 3984
MspA1I	CMGCKG	4 cut(s)	3931, 4456, 5315, 7258
MspCI	CTTAAG	4 cut(s)	1268, 2513, 6064, 7166
MunI	CAATTG	3 cut(s)	4013, 4683, 7736
Mva1269I	GAATGC	8 cut(s)	383, 385, 819, 3228, 3583, 5942, 5968, 5976
MvaI	CCWGG	7 cut(s)	2184, 2639, 4960, 6177, 6636, 6686, 7400
MvnI	CGCG	1 cut(s)	5399
NaeI	GCCGGC	1 cut(s)	3560
NciI	CCSGG	5 cut(s)	37, 83, 2980, 7015, 7016
NdeI	CATATG	4 cut(s)	729, 1319, 2692, 3796
NgoMIV	GCCGGC	1 cut(s)	3558
NmeAIII	GCCGAG	3 cut(s)	874, 2350, 7281
NmuCI	GTSAC	6 cut(s)	1116, 1656, 2667, 2779, 6754, 6821
NsiI	ATGCAT	3 cut(s)	385, 3230, 5813
NspV	TTCGAA	5 cut(s)	1702, 3049, 5536, 5890, 7610
PaeI	GCATGC	4 cut(s)	902, 5914, 6011, 7730
PagI	TCATGA	3 cut(s)	243, 2986, 7183
PaqCI	CACCTGC	2 cut(s)	5179, 6829
PceI	AGGCCT	1 cut(s)	3381
PciI	ACATGT	2 cut(s)	304, 5263
PcsI	WCGNNNNNNNCGW	2 cut(s)	111, 1309
PctI	GAATGC	8 cut(s)	383, 385, 819, 3228, 3583, 5942, 5968, 5976
PdiI	GCCGGC	1 cut(s)	3560
PdmI	GAANNNNTTC	9 cut(s)	21, 383, 708, 744, 3055, 3924, 5092, 5991, 7758
Pfl23II	CGTACG	1 cut(s)	937
PfoI	TCCNGGA	2 cut(s)	81, 7398
PinAI	ACCGGT	1 cut(s)	6825
PleI	GAGTC	6 cut(s)	136, 1844, 2335, 2865, 5239, 5935
PmaCI	CACGTG	2 cut(s)	2501, 5699
PmlI	CACGTG	2 cut(s)	2501, 5699
PpsI	GAGTC	6 cut(s)	136, 1844, 2335, 2865, 5239, 5935
Ppu21I	YACGTR	5 cut(s)	2501, 2534, 5240, 5699, 6378
PpuMI	RGGWCCY	5 cut(s)	2383, 2906, 5154, 6620, 7748
PscI	ACATGT	2 cut(s)	304, 5263
PshBI	ATTAAT	2 cut(s)	3690, 3959
PsiI	TTATAA	2 cut(s)	1716, 7485
Psp5II	RGGWCCY	5 cut(s)	2383, 2906, 5154, 6620, 7748
Psp6I	CCWGG	7 cut(s)	2182, 2637, 4958, 6175, 6634, 6684, 7398
PspCI	CACGTG	2 cut(s)	2501, 5699
PspEI	GGTNACC	2 cut(s)	2667, 6821
PspFI	CCCAGC	3 cut(s)	3553, 6170, 7254
PspGI	CCWGG	7 cut(s)	2182, 2637, 4958, 6175, 6634, 6684, 7398
PspLI	CGTACG	1 cut(s)	937
PspPPI	RGGWCCY	5 cut(s)	2383, 2906, 5154, 6620, 7748
PstI	CTGCAG	4 cut(s)	3144, 3331, 3433, 7056
PstNI	CAGNNNCTG	5 cut(s)	1337, 3326, 3923, 4895, 5194
PvuII	CAGCTG	2 cut(s)	4456, 7258
RseI	CAYNNNNRTG	9 cut(s)	309, 903, 1607, 2037, 2168, 2991, 7185, 7188, 7827
SalI	GTCGAC	3 cut(s)	2455, 2625, 6580
ScaI	AGTACT	3 cut(s)	2122, 5057, 5455
SchI	GAGTC	6 cut(s)	137, 1844, 2336, 2866, 5240, 5936
SduI	GDGCHC	4 cut(s)	928, 4447, 5704, 7723
SfuI	TTCGAA	5 cut(s)	1702, 3049, 5536, 5890, 7610
SmaI	CCCGGG	1 cut(s)	7016
SmiMI	CAYNNNNRTG	9 cut(s)	309, 903, 1607, 2037, 2168, 2991, 7185, 7188, 7827
SmlI	CTYRAG	9 cut(s)	589, 598, 656, 1268, 2513, 5016, 6064, 6970, 7166
SmoI	CTYRAG	9 cut(s)	589, 598, 656, 1268, 2513, 5016, 6064, 6970, 7166
SpeI	ACTAGT	1 cut(s)	6758
SphI	GCATGC	4 cut(s)	902, 5914, 6011, 7730
SseBI	AGGCCT	1 cut(s)	3381
SspI	AATATT	7 cut(s)	156, 639, 1671, 2062, 4643, 6678, 7648
StuI	AGGCCT	1 cut(s)	3381
StyI	CCWWGG	8 cut(s)	1009, 1545, 3041, 5150, 5767, 6534, 6833, 7458
TaiI	ACGT	9 cut(s)	2195, 2503, 2536, 3735, 4165, 5242, 5294, 5701, 6380
TaqII	GACCGA	3 cut(s)	31, 3237, 7277
TauI	GCSGC	4 cut(s)	8, 5686, 6728, 7637
TseFI	GTSAC	6 cut(s)	1116, 1656, 2667, 2779, 6754, 6821
Tsp45I	GTSAC	6 cut(s)	1116, 1656, 2667, 2779, 6754, 6821
TspGWI	ACGGA	6 cut(s)	19, 3407, 3500, 3735, 4812, 7380
TspMI	CCCGGG	1 cut(s)	7014
Vha464I	CTTAAG	4 cut(s)	1268, 2513, 6064, 7166
VneI	GTGCAC	2 cut(s)	924, 5700
VspI	ATTAAT	2 cut(s)	3690, 3959
XagI	CCTNNNNNAGG	1 cut(s)	5677
XbaI	TCTAGA	1 cut(s)	2714
XmaI	CCCGGG	1 cut(s)	7014
XmaJI	CCTAGG	1 cut(s)	3041
XmiI	GTMKAC	8 cut(s)	1309, 2316, 2456, 2626, 5794, 6581, 6943, 7155
XmnI	GAANNNNTTC	9 cut(s)	21, 383, 708, 744, 3055, 3924, 5092, 5991, 7758
ZrmI	AGTACT	3 cut(s)	2122, 5057, 5455
Zsp2I	ATGCAT	3 cut(s)	385, 3230, 5813

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.