FvH4_4g20390

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014719
Physical Location & Seq
Reverse (-)
23675322 .. 23676020
699 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20390.t1

Sequence Viewer

Length: 498 bp
ATGTCAAGGATCAAGGAGATTGTACAGGTTCTGGTTGGGCATTTTCAACAGCAGCAGCAGCGGAAGGGCTTACCCCATCGGGGTCTTTGGATTAGAGGAGCTATGGCTTCGATTAGAGGAGCTATGACTTATGTAAGGGATTATGGACTCGTAACTAAAGAACAGCTTCCTTACATGTTTGCTGATGACCAACCATGCAACACCGATTTCGAATTGTTACCTTCCCCTGATATGAAGATATCTGGTTATCAAATGGTGCCGGAGTTTGATGAAGTTGCTCTGGCCAAGGCTGTTTACCATCAACCAGTTGTAGACAAGCGTTCCACAAGCAACAGAATTCACACACACAAAAACGGCATTTTTATGGATGCAGATGGAGTGTGTGCAAAAGGTAACGTCAGTCACAGTAGTACACTCGTTGGCTTCGGTACAAATCCATTTGGTGAAGACTATTGGATTCAGAAGAATTCATGGGGCACCTGTTGGGGTGAACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

166

Amino Acids

18.65

Weight (kDa)

7.01

Isoelectric Point (pI)

30.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C1 PF00112 40 - 164 4.8e-20 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 256, 476
AccI GTMKAC 1 cut(s) 312
AciI CCGC 1 cut(s) 61
AclWI GGATC 1 cut(s) 17
AcoI YGGCCR 1 cut(s) 282
AcsI RAATTY 2 cut(s) 336, 466
AfaI GTAC 3 cut(s) 24, 412, 430
AfiI CCNNNNNNNGG 1 cut(s) 80
AflIII ACRYGT 1 cut(s) 174
AgsI TTSAA 1 cut(s) 47
AluBI AGCT 3 cut(s) 101, 122, 166
AluI AGCT 3 cut(s) 101, 122, 166
AlwI GGATC 1 cut(s) 17
AlwNI CAGNNNCTG 1 cut(s) 31
AoxI GGCC 1 cut(s) 282
ApeKI GCWGC 3 cut(s) 52, 55, 58
ApoI RAATTY 2 cut(s) 336, 466
Asp700I GAANNNNTTC 1 cut(s) 165
AsuHPI GGTGA 1 cut(s) 455
AsuII TTCGAA 1 cut(s) 210
BaeGI GKGCMC 1 cut(s) 479
BalI TGGCCA 1 cut(s) 284
BanI GGYRCC 2 cut(s) 256, 476
BbsI GAAGAC 1 cut(s) 453
BbvI GCAGC 3 cut(s) 64, 67, 70
BccI CCATC 3 cut(s) 84, 306, 368
BceAI ACGGC 1 cut(s) 370
BisI GCNGC 3 cut(s) 53, 56, 59
BlsI GCNGC 3 cut(s) 54, 57, 60
BmiI GGNNCC 2 cut(s) 258, 478
BmsI GCATC 1 cut(s) 358
BpiI GAAGAC 1 cut(s) 453
Bpu14I TTCGAA 1 cut(s) 210
BsaJI CCNNGG 1 cut(s) 285
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse1I ACTGG 1 cut(s) 305
BseDI CCNNGG 1 cut(s) 285
BseGI GGATG 1 cut(s) 373
BseLI CCNNNNNNNGG 1 cut(s) 80
BseNI ACTGG 1 cut(s) 305
BseRI GAGGAG 2 cut(s) 111, 132
BseSI GKGCMC 1 cut(s) 479
BseXI GCAGC 3 cut(s) 64, 67, 70
BshFI GGCC 1 cut(s) 284
BshNI GGYRCC 2 cut(s) 256, 476
BsiSI CCGG 1 cut(s) 260
BslI CCNNNNNNNGG 1 cut(s) 80
BsnI GGCC 1 cut(s) 284
Bsp119I TTCGAA 1 cut(s) 210
Bsp1286I GDGCHC 1 cut(s) 479
Bsp1407I TGTACA 1 cut(s) 22
Bsp143I GATC 1 cut(s) 9
BspACI CCGC 1 cut(s) 61
BspANI GGCC 1 cut(s) 284
BspLI GGNNCC 2 cut(s) 258, 478
BspPI GGATC 1 cut(s) 17
BspT104I TTCGAA 1 cut(s) 210
BspT107I GGYRCC 2 cut(s) 256, 476
BsrGI TGTACA 1 cut(s) 22
BsrI ACTGG 1 cut(s) 305
BssECI CCNNGG 1 cut(s) 285
BssMI GATC 1 cut(s) 9
BssT1I CCWWGG 1 cut(s) 285
Bst4CI ACNGT 1 cut(s) 407
BstAUI TGTACA 1 cut(s) 22
BstBI TTCGAA 1 cut(s) 210
BstF5I GGATG 1 cut(s) 373
BstKTI GATC 1 cut(s) 12
BstMBI GATC 1 cut(s) 9
BstMWI GCNNNNNNNGC 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 178
BstSLI GKGCMC 1 cut(s) 479
BstV1I GCAGC 3 cut(s) 64, 67, 70
BstV2I GAAGAC 1 cut(s) 453
BsuRI GGCC 1 cut(s) 284
BtsCI GGATG 1 cut(s) 373
CaiI CAGNNNCTG 1 cut(s) 31
Csp6I GTAC 3 cut(s) 23, 411, 429
CviAII CATG 3 cut(s) 175, 195, 471
CviJI RGCY 8 cut(s) 69, 101, 107, 122, 166, 284, 290, 423
CviKI_1 RGCY 8 cut(s) 69, 101, 107, 122, 166, 284, 290, 423
CviQI GTAC 3 cut(s) 23, 411, 429
DpnI GATC 1 cut(s) 11
DpnII GATC 1 cut(s) 9
EaeI YGGCCR 1 cut(s) 282
Eco130I CCWWGG 1 cut(s) 285
Eco32I GATATC 1 cut(s) 240
EcoRI GAATTC 2 cut(s) 336, 466
EcoRV GATATC 1 cut(s) 240
EcoT14I CCWWGG 1 cut(s) 285
ErhI CCWWGG 1 cut(s) 285
FaeI CATG 3 cut(s) 178, 198, 474
FalI AAGNNNNNCTT 2 cut(s) 150, 182
FatI CATG 3 cut(s) 174, 194, 470
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 3 cut(s) 53, 56, 59
FokI GGATG 1 cut(s) 380
Fsp4HI GCNGC 3 cut(s) 53, 56, 59
GluI GCNGC 3 cut(s) 53, 56, 59
HaeIII GGCC 1 cut(s) 284
HapII CCGG 1 cut(s) 260
Hin1II CATG 3 cut(s) 178, 198, 474
HinfI GANTC 2 cut(s) 147, 457
HpaII CCGG 1 cut(s) 260
HphI GGTGA 1 cut(s) 455
Hpy166II GTNNAC 4 cut(s) 295, 313, 413, 491
Hpy188I TCNGA 1 cut(s) 462
Hpy8I GTNNAC 4 cut(s) 295, 313, 413, 491
HpyAV CCTTC 2 cut(s) 58, 231
HpyCH4III ACNGT 1 cut(s) 407
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 3 cut(s) 198, 371, 386
HpyF10VI GCNNNNNNNGC 1 cut(s) 58
HpySE526I ACGT 1 cut(s) 396
Hsp92II CATG 3 cut(s) 178, 198, 474
Kzo9I GATC 1 cut(s) 9
LmnI GCTCC 2 cut(s) 98, 119
LpnPI CCDG 8 cut(s) 11, 17, 228, 240, 266, 273, 318, 493
Lsp1109I GCAGC 3 cut(s) 64, 67, 70
LweI GCATC 1 cut(s) 358
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 4 cut(s) 151, 216, 392, 401
MalI GATC 1 cut(s) 11
MboI GATC 1 cut(s) 9
MboII GAAGA 3 cut(s) 247, 458, 475
MhlI GDGCHC 1 cut(s) 479
MlsI TGGCCA 1 cut(s) 284
MluCI AATT 3 cut(s) 212, 336, 466
MluNI TGGCCA 1 cut(s) 284
MlyI GAGTC 1 cut(s) 141
MnlI CCTC 2 cut(s) 89, 110
Mox20I TGGCCA 1 cut(s) 284
MroXI GAANNNNTTC 1 cut(s) 165
MscI TGGCCA 1 cut(s) 284
MslI CAYNNNNRTG 1 cut(s) 362
Msp20I TGGCCA 1 cut(s) 284
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 1 cut(s) 260
MwoI GCNNNNNNNGC 1 cut(s) 58
NdeII GATC 1 cut(s) 9
NlaIII CATG 3 cut(s) 178, 198, 474
NlaIV GGNNCC 2 cut(s) 258, 478
NmuCI GTSAC 1 cut(s) 401
NspI RCATGY 1 cut(s) 178
NspV TTCGAA 1 cut(s) 210
PciI ACATGT 1 cut(s) 174
PdmI GAANNNNTTC 1 cut(s) 165
PfeI GAWTC 1 cut(s) 457
PkrI GCNGC 3 cut(s) 54, 57, 60
PleI GAGTC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 141
PscI ACATGT 1 cut(s) 174
PspN4I GGNNCC 2 cut(s) 258, 478
PstNI CAGNNNCTG 1 cut(s) 31
RsaI GTAC 3 cut(s) 24, 412, 430
RsaNI GTAC 3 cut(s) 23, 411, 429
RseI CAYNNNNRTG 1 cut(s) 362
SatI GCNGC 3 cut(s) 53, 56, 59
Sau3AI GATC 1 cut(s) 9
SchI GAGTC 1 cut(s) 141
SduI GDGCHC 1 cut(s) 479
SetI ASST 8 cut(s) 30, 103, 124, 168, 223, 394, 399, 482
SfaNI GCATC 1 cut(s) 358
SfuI TTCGAA 1 cut(s) 210
SmiMI CAYNNNNRTG 1 cut(s) 362
Sse9I AATT 3 cut(s) 212, 336, 466
SsiI CCGC 1 cut(s) 61
StyI CCWWGG 1 cut(s) 285
TaaI ACNGT 1 cut(s) 407
TaiI ACGT 1 cut(s) 399
TaqI TCGA 2 cut(s) 110, 210
TasI AATT 3 cut(s) 212, 336, 466
TatI WGTACW 2 cut(s) 22, 410
TfiI GAWTC 1 cut(s) 457
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 3 cut(s) 52, 55, 58
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 3 cut(s) 248, 285, 459
XapI RAATTY 2 cut(s) 336, 466
XceI RCATGY 1 cut(s) 178
XmiI GTMKAC 1 cut(s) 312
XmnI GAANNNNTTC 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.