FvH4_5g18392

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014719
Physical Location & Seq
Forward (+)
10574352 .. 10577181
2830 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18392.t1

Sequence Viewer

Length: 1404 bp
ATGACTTCCCAAGTAAAATCTTATCTACTCTTATGCGTTGTCTTGATCGCAAGTTTAGCTCTACTGTCTAGAGCCACATCGCGATCATTAACTTCTGAAGCTAATGATTCCGTGAATGTTTCTCAAGTATTTGACAACTGGGTGGAAGAGTATGGACGCACTTACTCGAGCAGCGAGGAGAAGGCAAAACGTTTTGCTATGTTCAAGGAGCGGTTTGAAGATATAGAAAAGTTCAACCGCCAAGGGAATCATACTTTCACATTAGGCCTCGATGCATTCAGTGATATGACTTTCGAAGAGTTCTCCACAATTGATATACCAAACCCTAGCTCAATCTCTTCTTTTAGGTATCAAGATTTTAAACTTGAGGATCTTCCCAAAAGCTGGGACTGGAGGACAGAAGGAGCTGTGACCAATGTTAAGGATCAAGGAAATTGTACTGTGCAGAAACTTATAGCCTGTAGTAAGCCCTACGGCACCCGTGGCTGTGAAGGAAATCTTCCGATTAGAGGAGCTATGGCTTCGATTAGAGGAGCTATGACTTATGCAAGGGATTATGGACTCGTAACTGAAGAAAAGCTTCCTTACATGTTTGCTGATGACCAACCATGCAACAACGATTTCGAATCTTTACCTGATATGAAGATATCTGGTTATCAAGTTGTGCCGGAGTTTGATGAAGTTGCTCTGGCCAAGGCTGTTTACCATCAACCAGTTGTAGTCATGCATTCCACAAGCGAAAGGTTTAACACACACAGAAGAGGTATTTTTATGGATGCAGATGGAGAGTGTGCGAAAGGTAAACTCAGTCACACTAGTACACTCGTTGGCTTCGGTACAACTCCATTTGGTCAAGAGTATTGGATTCTGAAGAATTCATGGGGCACCCGTTGGGGTGAACATGGTTATATGAGAATCAATCGAAATTCGAAAAATAAGCGAGACAAACCCCTGATCAAGGGTCTCTCAAACCAGCTCCGCAGAACTCGGCGTTCTCTAGGGTTTTCTCAAAAGTTTTTGTCTCTCCGTTCGACAACAGCATCTGTTGACGTCGTCGTCGGACGAGATTTCATTGATAGTCGTTTGGTGATCAGGCTCTATGGGTTTGTGGCGGTGGAAGGAGAAGATTCTTTGCTTTTGCTTGGTATGTTGAGAATCTCTTGCATCGGCGACAAATGTATGGCTGCCCGGATGGGGTTTCTCGATCTGCTTTTGCTCTCTTTGTTGCATATTGGTGCACACTTTGGAAGGTTGGGCTCTCCAACGCCGTGTGACAAGGCGGTGAACCTCTACTGGTGGCGGTCAAAATCGGTGGCGGAGCGGTTTTACTGTAGGCGGTGGTGGCTGCTTGGTGTGTCTTCTTGGCATGATCCTTTTTGGTCCAATGGGTGGGTTAGGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

468

Amino Acids

53.13

Weight (kDa)

8.78

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 44 - 101 5.1e-18 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 149 - 312 2.5e-33 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1055
AatII GACGTC 1 cut(s) 1053
AccB1I GGYRCC 2 cut(s) 476, 884
AccB7I CCANNNNNTGG 2 cut(s) 384, 1389
AccBSI CCGCTC 2 cut(s) 211, 1321
AccII CGCG 1 cut(s) 82
AciI CCGC 9 cut(s) 211, 238, 979, 1112, 1280, 1300, 1316, 1321, 1336
AclI AACGTT 1 cut(s) 190
AclWI GGATC 3 cut(s) 378, 432, 1364
AcoI YGGCCR 1 cut(s) 690
AcsI RAATTY 2 cut(s) 874, 925
AcuI CTGAAG 3 cut(s) 117, 591, 890
AcyI GRCGYC 1 cut(s) 1050
AfaI GTAC 3 cut(s) 439, 820, 838
AfiI CCNNNNNNNGG 5 cut(s) 384, 509, 958, 1194, 1389
AflIII ACRYGT 1 cut(s) 588
AgsI TTSAA 3 cut(s) 205, 218, 235
AhlI ACTAGT 1 cut(s) 815
AluBI AGCT 9 cut(s) 59, 101, 330, 384, 407, 515, 536, 580, 976
AluI AGCT 9 cut(s) 59, 101, 330, 384, 407, 515, 536, 580, 976
Alw21I GWGCWC 1 cut(s) 1240
Alw26I GTCTC 3 cut(s) 936, 968, 1026
Alw44I GTGCAC 1 cut(s) 1236
AlwI GGATC 3 cut(s) 378, 432, 1364
AlwNI CAGNNNCTG 1 cut(s) 1043
Ama87I CYCGRG 1 cut(s) 166
AoxI GGCC 3 cut(s) 265, 690, 1398
ApaLI GTGCAC 1 cut(s) 1236
ApeKI GCWGC 3 cut(s) 171, 1184, 1345
ApoI RAATTY 2 cut(s) 874, 925
Asp700I GAANNNNTTC 1 cut(s) 579
AspS9I GGNCC 2 cut(s) 1380, 1398
AsuC2I CCSGG 1 cut(s) 1189
AsuHPI GGTGA 3 cut(s) 908, 1099, 1294
AsuII TTCGAA 3 cut(s) 294, 624, 929
AvaI CYCGRG 1 cut(s) 166
AvaII GGWCC 1 cut(s) 1380
BaeGI GKGCMC 2 cut(s) 887, 1240
BalI TGGCCA 1 cut(s) 692
BanI GGYRCC 2 cut(s) 476, 884
BanII GRGCYC 1 cut(s) 1259
BbsI GAAGAC 1 cut(s) 1350
Bbv12I GWGCWC 1 cut(s) 1240
BbvI GCAGC 3 cut(s) 183, 1171, 1332
BccI CCATC 3 cut(s) 714, 776, 1186
BceAI ACGGC 2 cut(s) 490, 1252
BclI TGATCA 2 cut(s) 954, 1089
BcnI CCSGG 1 cut(s) 1189
BcoDI GTCTC 3 cut(s) 936, 968, 1026
BcuI ACTAGT 1 cut(s) 815
BfaI CTAG 5 cut(s) 69, 327, 816, 998, 1402
BfmI CTRYAG 2 cut(s) 460, 1330
BisI GCNGC 3 cut(s) 172, 1185, 1346
BlsI GCNGC 3 cut(s) 173, 1186, 1347
Bme1390I CCNGG 1 cut(s) 1189
Bme18I GGWCC 1 cut(s) 1380
BmeT110I CYCGRG 1 cut(s) 166
BmgT120I GGNCC 2 cut(s) 1380, 1398
BmiI GGNNCC 2 cut(s) 478, 886
BmrFI CCNGG 1 cut(s) 1189
BmrI ACTGGG 1 cut(s) 148
BmsI GCATC 4 cut(s) 262, 766, 1049, 1173
BmuI ACTGGG 1 cut(s) 148
BpiI GAAGAC 1 cut(s) 1350
BpmI CTGGAG 1 cut(s) 412
Bpu14I TTCGAA 3 cut(s) 294, 624, 929
BpuEI CTTGAG 2 cut(s) 108, 386
BpuMI CCSGG 1 cut(s) 1189
BsaHI GRCGYC 1 cut(s) 1050
BsaI GGTCTC 1 cut(s) 968
BsaJI CCNNGG 3 cut(s) 241, 481, 693
Bsc4I CCNNNNNNNGG 5 cut(s) 384, 509, 958, 1194, 1389
Bse1I ACTGG 4 cut(s) 143, 395, 713, 1298
BseDI CCNNGG 3 cut(s) 241, 481, 693
BseGI GGATG 2 cut(s) 781, 1197
BseLI CCNNNNNNNGG 5 cut(s) 384, 509, 958, 1194, 1389
BseMII CTCAG 1 cut(s) 820
BseNI ACTGG 4 cut(s) 143, 395, 713, 1298
BseRI GAGGAG 3 cut(s) 191, 525, 546
BseSI GKGCMC 2 cut(s) 887, 1240
BseXI GCAGC 3 cut(s) 183, 1171, 1332
BseYI CCCAGC 1 cut(s) 384
BsgI GTGCAG 1 cut(s) 464
Bsh1236I CGCG 1 cut(s) 82
BshFI GGCC 3 cut(s) 267, 692, 1400
BshNI GGYRCC 2 cut(s) 476, 884
BsiHKAI GWGCWC 1 cut(s) 1240
BsiHKCI CYCGRG 1 cut(s) 166
BsiSI CCGG 2 cut(s) 668, 1189
BslFI GGGAC 1 cut(s) 401
BslI CCNNNNNNNGG 5 cut(s) 384, 509, 958, 1194, 1389
BsmAI GTCTC 3 cut(s) 936, 968, 1026
BsmFI GGGAC 1 cut(s) 401
BsmI GAATGC 2 cut(s) 275, 727
BsnI GGCC 3 cut(s) 267, 692, 1400
Bso31I GGTCTC 1 cut(s) 968
BsoBI CYCGRG 1 cut(s) 166
Bsp119I TTCGAA 3 cut(s) 294, 624, 929
Bsp1286I GDGCHC 3 cut(s) 887, 1240, 1259
Bsp143I GATC 8 cut(s) 45, 83, 370, 424, 954, 1089, 1204, 1369
Bsp68I TCGCGA 1 cut(s) 82
BspACI CCGC 9 cut(s) 211, 238, 979, 1112, 1280, 1300, 1316, 1321, 1336
BspANI GGCC 3 cut(s) 267, 692, 1400
BspCNI CTCAG 1 cut(s) 819
BspFNI CGCG 1 cut(s) 82
BspLI GGNNCC 2 cut(s) 478, 886
BspPI GGATC 3 cut(s) 378, 432, 1364
BspT104I TTCGAA 3 cut(s) 294, 624, 929
BspT107I GGYRCC 2 cut(s) 476, 884
BspTNI GGTCTC 1 cut(s) 968
BsrBI CCGCTC 2 cut(s) 211, 1321
BsrI ACTGG 4 cut(s) 143, 395, 713, 1298
BssECI CCNNGG 3 cut(s) 241, 481, 693
BssMI GATC 8 cut(s) 45, 83, 370, 424, 954, 1089, 1204, 1369
BssNI GRCGYC 1 cut(s) 1050
BssT1I CCWWGG 2 cut(s) 241, 693
Bst4CI ACNGT 3 cut(s) 66, 442, 1331
Bst6I CTCTTC 4 cut(s) 141, 291, 343, 754
BstACI GRCGYC 1 cut(s) 1050
BstBI TTCGAA 3 cut(s) 294, 624, 929
BstDEI CTNAG 1 cut(s) 806
BstDSI CCRYGG 1 cut(s) 481
BstENI CCTNNNNNAGG 1 cut(s) 956
BstF5I GGATG 2 cut(s) 781, 1197
BstFNI CGCG 1 cut(s) 82
BstKTI GATC 8 cut(s) 48, 86, 373, 427, 957, 1092, 1207, 1372
BstMAI GTCTC 3 cut(s) 936, 968, 1026
BstMBI GATC 8 cut(s) 45, 83, 370, 424, 954, 1089, 1204, 1369
BstMWI GCNNNNNNNGC 3 cut(s) 56, 483, 1342
BstNSI RCATGY 1 cut(s) 592
BstSCI CCNGG 1 cut(s) 1187
BstSFI CTRYAG 2 cut(s) 460, 1330
BstSLI GKGCMC 2 cut(s) 887, 1240
BstUI CGCG 1 cut(s) 82
BstV1I GCAGC 3 cut(s) 183, 1171, 1332
BstV2I GAAGAC 1 cut(s) 1350
BstX2I RGATCY 1 cut(s) 370
BstYI RGATCY 1 cut(s) 370
BsuRI GGCC 3 cut(s) 267, 692, 1400
BtgI CCRYGG 1 cut(s) 481
BtgZI GCGATG 1 cut(s) 63
BtsCI GGATG 2 cut(s) 781, 1197
BtsIMutI CAGTG 1 cut(s) 286
BtuMI TCGCGA 1 cut(s) 82
CaiI CAGNNNCTG 1 cut(s) 1043
Cfr13I GGNCC 2 cut(s) 1380, 1398
CseI GACGC 1 cut(s) 165
Csp6I GTAC 3 cut(s) 438, 819, 837
CspCI CAANNNNNGTGG 2 cut(s) 1293, 1328
CviAII CATG 6 cut(s) 589, 609, 724, 879, 902, 1367
CviQI GTAC 3 cut(s) 438, 819, 837
DdeI CTNAG 1 cut(s) 806
DpnI GATC 8 cut(s) 47, 85, 372, 426, 956, 1091, 1206, 1371
DpnII GATC 8 cut(s) 45, 83, 370, 424, 954, 1089, 1204, 1369
DraI TTTAAA 1 cut(s) 361
DrdI GACNNNNNNGTC 1 cut(s) 1055
DseDI GACNNNNNNGTC 1 cut(s) 1055
EaeI YGGCCR 1 cut(s) 690
Eam1104I CTCTTC 4 cut(s) 141, 291, 343, 754
EarI CTCTTC 4 cut(s) 141, 291, 343, 754
EciI GGCGGA 1 cut(s) 1331
Eco130I CCWWGG 2 cut(s) 241, 693
Eco147I AGGCCT 1 cut(s) 267
Eco24I GRGCYC 1 cut(s) 1259
Eco31I GGTCTC 1 cut(s) 968
Eco32I GATATC 1 cut(s) 648
Eco47I GGWCC 1 cut(s) 1380
Eco57I CTGAAG 3 cut(s) 117, 591, 890
Eco88I CYCGRG 1 cut(s) 166
EcoNI CCTNNNNNAGG 1 cut(s) 956
EcoO109I RGGNCCY 1 cut(s) 1398
EcoRI GAATTC 1 cut(s) 874
EcoRV GATATC 1 cut(s) 648
EcoT14I CCWWGG 2 cut(s) 241, 693
EcoT22I ATGCAT 2 cut(s) 277, 729
EcoT38I GRGCYC 1 cut(s) 1259
ErhI CCWWGG 2 cut(s) 241, 693
FaeI CATG 6 cut(s) 592, 612, 727, 882, 905, 1370
FalI AAGNNNNNCTT 4 cut(s) 483, 515, 564, 596
FaqI GGGAC 1 cut(s) 401
FatI CATG 6 cut(s) 588, 608, 723, 878, 901, 1366
FbaI TGATCA 2 cut(s) 954, 1089
Fnu4HI GCNGC 3 cut(s) 172, 1185, 1346
FokI GGATG 2 cut(s) 788, 1204
FriOI GRGCYC 1 cut(s) 1259
Fsp4HI GCNGC 3 cut(s) 172, 1185, 1346
FspBI CTAG 5 cut(s) 69, 327, 816, 998, 1402
GluI GCNGC 3 cut(s) 172, 1185, 1346
GsaI CCCAGC 1 cut(s) 388
GsuI CTGGAG 1 cut(s) 412
HaeIII GGCC 3 cut(s) 267, 692, 1400
HapII CCGG 2 cut(s) 668, 1189
HgaI GACGC 1 cut(s) 165
Hin1I GRCGYC 1 cut(s) 1050
Hin1II CATG 6 cut(s) 592, 612, 727, 882, 905, 1370
HincII GTYRAC 1 cut(s) 1048
HindII GTYRAC 1 cut(s) 1048
HindIII AAGCTT 1 cut(s) 578
HinfI GANTC 8 cut(s) 107, 247, 561, 626, 865, 915, 1127, 1155
HpaII CCGG 2 cut(s) 668, 1189
HphI GGTGA 3 cut(s) 908, 1099, 1294
Hpy166II GTNNAC 7 cut(s) 703, 803, 821, 899, 1048, 1238, 1285
Hpy188I TCNGA 4 cut(s) 97, 504, 870, 1061
Hpy188III TCNNGA 6 cut(s) 43, 69, 81, 353, 854, 1202
Hpy8I GTNNAC 7 cut(s) 703, 803, 821, 899, 1048, 1238, 1285
Hpy99I CGWCG 3 cut(s) 1055, 1058, 1061
HpyAV CCTTC 5 cut(s) 175, 395, 485, 1112, 1242
HpyCH4III ACNGT 3 cut(s) 66, 442, 1331
HpyCH4IV ACGT 2 cut(s) 190, 1050
HpyCH4V TGCA 9 cut(s) 275, 445, 548, 612, 727, 779, 1164, 1228, 1238
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 483, 1342
HpyF3I CTNAG 1 cut(s) 806
HpySE526I ACGT 2 cut(s) 190, 1050
Hsp92I GRCGYC 1 cut(s) 1050
Hsp92II CATG 6 cut(s) 592, 612, 727, 882, 905, 1370
Ksp22I TGATCA 2 cut(s) 954, 1089
Kzo9I GATC 8 cut(s) 45, 83, 370, 424, 954, 1089, 1204, 1369
LmnI GCTCC 6 cut(s) 208, 404, 512, 533, 981, 1318
Lsp1109I GCAGC 3 cut(s) 183, 1171, 1332
LweI GCATC 4 cut(s) 262, 766, 1049, 1173
MaeI CTAG 5 cut(s) 69, 327, 816, 998, 1402
MaeII ACGT 2 cut(s) 190, 1050
MaeIII GTNAC 4 cut(s) 409, 565, 809, 1271
MalI GATC 8 cut(s) 47, 85, 372, 426, 956, 1091, 1206, 1371
MbiI CCGCTC 2 cut(s) 211, 1321
MboI GATC 8 cut(s) 45, 83, 370, 424, 954, 1089, 1204, 1369
MfeI CAATTG 1 cut(s) 309
MflI RGATCY 1 cut(s) 370
MhlI GDGCHC 3 cut(s) 887, 1240, 1259
MlsI TGGCCA 1 cut(s) 692
MluCI AATT 4 cut(s) 309, 433, 874, 925
MluNI TGGCCA 1 cut(s) 692
MlyI GAGTC 1 cut(s) 555
MmeI TCCRAC 2 cut(s) 1039, 1286
MnlI CCTC 8 cut(s) 169, 278, 361, 387, 503, 524, 755, 1298
Mox20I TGGCCA 1 cut(s) 692
Mph1103I ATGCAT 2 cut(s) 277, 729
MroXI GAANNNNTTC 1 cut(s) 579
MscI TGGCCA 1 cut(s) 692
MseI TTAA 4 cut(s) 89, 360, 420, 747
MslI CAYNNNNRTG 1 cut(s) 1233
Msp20I TGGCCA 1 cut(s) 692
MspI CCGG 2 cut(s) 668, 1189
MspR9I CCNGG 1 cut(s) 1189
MunI CAATTG 1 cut(s) 309
Mva1269I GAATGC 2 cut(s) 275, 727
MvnI CGCG 1 cut(s) 82
MwoI GCNNNNNNNGC 3 cut(s) 56, 483, 1342
NciI CCSGG 1 cut(s) 1189
NdeII GATC 8 cut(s) 45, 83, 370, 424, 954, 1089, 1204, 1369
NlaIII CATG 6 cut(s) 592, 612, 727, 882, 905, 1370
NlaIV GGNNCC 2 cut(s) 478, 886
NmeAIII GCCGAG 1 cut(s) 967
NmuCI GTSAC 3 cut(s) 409, 809, 1271
NruI TCGCGA 1 cut(s) 82
NsiI ATGCAT 2 cut(s) 277, 729
NspI RCATGY 1 cut(s) 592
NspV TTCGAA 3 cut(s) 294, 624, 929
PaeR7I CTCGAG 1 cut(s) 166
PceI AGGCCT 1 cut(s) 267
PciI ACATGT 1 cut(s) 588
PctI GAATGC 2 cut(s) 275, 727
PdmI GAANNNNTTC 1 cut(s) 579
PfeI GAWTC 7 cut(s) 107, 247, 626, 865, 915, 1127, 1155
PflFI GACNNNGTC 1 cut(s) 1052
PflMI CCANNNNNTGG 2 cut(s) 384, 1389
PkrI GCNGC 3 cut(s) 173, 1186, 1347
PleI GAGTC 1 cut(s) 555
PpsI GAGTC 1 cut(s) 555
PscI ACATGT 1 cut(s) 588
Psp1406I AACGTT 1 cut(s) 190
PspFI CCCAGC 1 cut(s) 384
PspN4I GGNNCC 2 cut(s) 478, 886
PspPI GGNCC 2 cut(s) 1380, 1398
PspXI VCTCGAGB 1 cut(s) 166
PstNI CAGNNNCTG 1 cut(s) 1043
PsuI RGATCY 1 cut(s) 370
PsyI GACNNNGTC 1 cut(s) 1052
RruI TCGCGA 1 cut(s) 82
RsaI GTAC 3 cut(s) 439, 820, 838
RsaNI GTAC 3 cut(s) 438, 819, 837
RseI CAYNNNNRTG 1 cut(s) 1233
SaqAI TTAA 4 cut(s) 89, 360, 420, 747
SatI GCNGC 3 cut(s) 172, 1185, 1346
Sau3AI GATC 8 cut(s) 45, 83, 370, 424, 954, 1089, 1204, 1369
Sau96I GGNCC 2 cut(s) 1380, 1398
SchI GAGTC 1 cut(s) 555
ScrFI CCNGG 1 cut(s) 1189
SduI GDGCHC 3 cut(s) 887, 1240, 1259
SfaNI GCATC 4 cut(s) 262, 766, 1049, 1173
SfcI CTRYAG 2 cut(s) 460, 1330
Sfr274I CTCGAG 1 cut(s) 166
SfuI TTCGAA 3 cut(s) 294, 624, 929
SinI GGWCC 1 cut(s) 1380
SlaI CTCGAG 1 cut(s) 166
SmiMI CAYNNNNRTG 1 cut(s) 1233
SmlI CTYRAG 3 cut(s) 123, 166, 365
SmoI CTYRAG 3 cut(s) 123, 166, 365
SpeI ACTAGT 1 cut(s) 815
Sse9I AATT 4 cut(s) 309, 433, 874, 925
SseBI AGGCCT 1 cut(s) 267
SsiI CCGC 9 cut(s) 211, 238, 979, 1112, 1280, 1300, 1316, 1321, 1336
SspMI CTAG 5 cut(s) 69, 327, 816, 998, 1402
StuI AGGCCT 1 cut(s) 267
StyD4I CCNGG 1 cut(s) 1187
StyI CCWWGG 2 cut(s) 241, 693
TaaI ACNGT 3 cut(s) 66, 442, 1331
TaiI ACGT 2 cut(s) 193, 1053
TaqI TCGA 9 cut(s) 167, 270, 294, 524, 624, 922, 929, 1031, 1203
TasI AATT 4 cut(s) 309, 433, 874, 925
TatI WGTACW 2 cut(s) 437, 818
TfiI GAWTC 7 cut(s) 107, 247, 626, 865, 915, 1127, 1155
Tru1I TTAA 4 cut(s) 89, 360, 420, 747
Tru9I TTAA 4 cut(s) 89, 360, 420, 747
TscAI CASTG 1 cut(s) 286
TseFI GTSAC 3 cut(s) 409, 809, 1271
TseI GCWGC 3 cut(s) 171, 1184, 1345
Tsp45I GTSAC 3 cut(s) 409, 809, 1271
TspDTI ATGAA 4 cut(s) 656, 693, 867, 1060
TspGWI ACGGA 2 cut(s) 100, 1016
TspRI CASTG 1 cut(s) 286
Tth111I GACNNNGTC 1 cut(s) 1052
Van91I CCANNNNNTGG 2 cut(s) 384, 1389
VneI GTGCAC 1 cut(s) 1236
VpaK11BI GGWCC 1 cut(s) 1380
XagI CCTNNNNNAGG 1 cut(s) 956
XapI RAATTY 2 cut(s) 874, 925
XbaI TCTAGA 1 cut(s) 68
XceI RCATGY 1 cut(s) 592
XhoI CTCGAG 1 cut(s) 166
XmnI GAANNNNTTC 1 cut(s) 579
XspI CTAG 5 cut(s) 69, 327, 816, 998, 1402
ZraI GACGTC 1 cut(s) 1051
Zsp2I ATGCAT 2 cut(s) 277, 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.