Rroxscaffold_1G00065800

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014719
Physical Location & Seq
Forward (+)
87388086 .. 87390974
2889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00065800.1

Sequence Viewer

Length: 543 bp
ATGGATCAGCTACGTAATGTGGATTGCATTGTTTATGCCATGGGCCCCCTCTTTACTTCTGTTTGCCCATCACTGAATGGTGAAGAGGGTTGTCAGTTCAGAGGAAGTTTAGCAGTGTCTAAAGCCTCGTCGCGAGCATTACTTTTTGAAGCTAATGATTCCATGAATATTTTGCAAGCATTTGAGAACTGGATGAACGAGCATGGACGCACTTACTCAAGCAGTGAGGAGAAGGCAAAGCGTTTTGCAATATTCAAGGAGGAGTTTGATCATATAGAAAACTTCAACAGCCAAGGGAAACATAGATTCACATTAGGCCTCAATGCATTCAGTGATCTGACTGATAAAGAGTTCGATGAGACAGATGGTTGCACGACTGCTATATCAAACACAAACCCTAGCTCAGTCTCTTCTTTTAGCTATCAAGATTTTGAAATTGAGGATCTTCCCCAAAACTGGGACTGGAGGACACAAGGAGCTGTGACCAGTGTCAAGGACCAAGGAAAATGTGGTAAGAGAAGGAAATCCCTAAATTTTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

180

Amino Acids

20.36

Weight (kDa)

5.0

Isoelectric Point (pI)

46.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 61 - 118 1.1e-20 Cathepsin propeptide inhibitor domain (I29)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 133
AclWI GGATC 2 cut(s) 12, 450
AcsI RAATTY 1 cut(s) 532
AfiI CCNNNNNNNGG 2 cut(s) 456, 457
AgsI TTSAA 4 cut(s) 149, 256, 286, 434
AluBI AGCT 5 cut(s) 10, 152, 402, 420, 479
AluI AGCT 5 cut(s) 10, 152, 402, 420, 479
Alw26I GTCTC 2 cut(s) 353, 412
AlwI GGATC 2 cut(s) 12, 450
AoxI GGCC 2 cut(s) 43, 316
ApaI GGGCCC 1 cut(s) 47
ApoI RAATTY 1 cut(s) 532
AspS9I GGNCC 3 cut(s) 43, 44, 496
AsuHPI GGTGA 1 cut(s) 92
AvaII GGWCC 1 cut(s) 496
BaeGI GKGCMC 1 cut(s) 47
BanII GRGCYC 1 cut(s) 47
BccI CCATC 2 cut(s) 76, 359
BclI TGATCA 1 cut(s) 268
BcoDI GTCTC 2 cut(s) 353, 412
BfaI CTAG 1 cut(s) 399
Bme18I GGWCC 1 cut(s) 496
BmgT120I GGNCC 3 cut(s) 43, 44, 496
BmiI GGNNCC 2 cut(s) 45, 46
BmrI ACTGGG 1 cut(s) 466
BmuI ACTGGG 1 cut(s) 466
BoxI GACNNNNGTC 1 cut(s) 488
BpmI CTGGAG 1 cut(s) 484
BpuEI CTTGAG 1 cut(s) 202
BsaAI YACGTR 1 cut(s) 14
BsaJI CCNNGG 3 cut(s) 39, 292, 499
Bsc4I CCNNNNNNNGG 2 cut(s) 456, 457
Bse1I ACTGG 4 cut(s) 194, 461, 467, 486
BseDI CCNNGG 3 cut(s) 39, 292, 499
BseGI GGATG 1 cut(s) 198
BseLI CCNNNNNNNGG 2 cut(s) 456, 457
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 4 cut(s) 194, 461, 467, 486
BseRI GAGGAG 2 cut(s) 242, 275
BseSI GKGCMC 1 cut(s) 47
Bsh1236I CGCG 1 cut(s) 133
BshFI GGCC 2 cut(s) 45, 318
BslFI GGGAC 1 cut(s) 473
BslI CCNNNNNNNGG 2 cut(s) 456, 457
BsmAI GTCTC 2 cut(s) 353, 412
BsmFI GGGAC 1 cut(s) 473
BsmI GAATGC 1 cut(s) 326
BsnI GGCC 2 cut(s) 45, 318
Bsp120I GGGCCC 1 cut(s) 43
Bsp1286I GDGCHC 1 cut(s) 47
Bsp143I GATC 4 cut(s) 4, 268, 334, 442
Bsp19I CCATGG 1 cut(s) 39
Bsp68I TCGCGA 1 cut(s) 133
BspANI GGCC 2 cut(s) 45, 318
BspCNI CTCAG 1 cut(s) 416
BspFNI CGCG 1 cut(s) 133
BspLI GGNNCC 2 cut(s) 45, 46
BspPI GGATC 2 cut(s) 12, 450
BsrI ACTGG 4 cut(s) 194, 461, 467, 486
BssECI CCNNGG 3 cut(s) 39, 292, 499
BssMI GATC 4 cut(s) 4, 268, 334, 442
BssT1I CCWWGG 3 cut(s) 39, 292, 499
Bst6I CTCTTC 2 cut(s) 78, 415
BstBAI YACGTR 1 cut(s) 14
BstC8I GCNNGC 2 cut(s) 135, 177
BstDEI CTNAG 1 cut(s) 403
BstDSI CCRYGG 1 cut(s) 39
BstF5I GGATG 1 cut(s) 198
BstFNI CGCG 1 cut(s) 133
BstKTI GATC 4 cut(s) 7, 271, 337, 445
BstMAI GTCTC 2 cut(s) 353, 412
BstMBI GATC 4 cut(s) 4, 268, 334, 442
BstPAI GACNNNNGTC 1 cut(s) 488
BstSLI GKGCMC 1 cut(s) 47
BstSNI TACGTA 1 cut(s) 14
BstUI CGCG 1 cut(s) 133
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
BsuRI GGCC 2 cut(s) 45, 318
BtgI CCRYGG 1 cut(s) 39
BtsCI GGATG 1 cut(s) 198
BtsI GCAGTG 2 cut(s) 120, 229
BtsIMutI CAGTG 5 cut(s) 71, 120, 229, 337, 493
BtuMI TCGCGA 1 cut(s) 133
Cac8I GCNNGC 2 cut(s) 135, 177
Cfr13I GGNCC 3 cut(s) 43, 44, 496
CseI GACGC 1 cut(s) 216
CviAII CATG 3 cut(s) 40, 163, 203
CviJI RGCY 9 cut(s) 10, 45, 125, 152, 291, 318, 402, 420, 479
CviKI_1 RGCY 9 cut(s) 10, 45, 125, 152, 291, 318, 402, 420, 479
DdeI CTNAG 1 cut(s) 403
DpnI GATC 4 cut(s) 6, 270, 336, 444
DpnII GATC 4 cut(s) 4, 268, 334, 442
Eam1104I CTCTTC 2 cut(s) 78, 415
EarI CTCTTC 2 cut(s) 78, 415
Eco105I TACGTA 1 cut(s) 14
Eco130I CCWWGG 3 cut(s) 39, 292, 499
Eco147I AGGCCT 1 cut(s) 318
Eco24I GRGCYC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 496
EcoO109I RGGNCCY 1 cut(s) 44
EcoT14I CCWWGG 3 cut(s) 39, 292, 499
EcoT22I ATGCAT 1 cut(s) 328
EcoT38I GRGCYC 1 cut(s) 47
ErhI CCWWGG 3 cut(s) 39, 292, 499
FaeI CATG 3 cut(s) 43, 166, 206
FaiI YATR 8 cut(s) 36, 41, 164, 204, 273, 275, 303, 383
FaqI GGGAC 1 cut(s) 473
FatI CATG 3 cut(s) 39, 162, 202
FbaI TGATCA 1 cut(s) 268
FokI GGATG 1 cut(s) 205
FriOI GRGCYC 1 cut(s) 47
FspBI CTAG 1 cut(s) 399
GsuI CTGGAG 1 cut(s) 484
HaeIII GGCC 2 cut(s) 45, 318
HgaI GACGC 1 cut(s) 216
Hin1II CATG 3 cut(s) 43, 166, 206
HinfI GANTC 2 cut(s) 158, 306
HphI GGTGA 1 cut(s) 92
Hpy188I TCNGA 2 cut(s) 101, 339
Hpy188III TCNNGA 2 cut(s) 132, 425
Hpy99I CGWCG 1 cut(s) 133
HpyAV CCTTC 2 cut(s) 226, 513
HpyCH4IV ACGT 1 cut(s) 13
HpyCH4V TGCA 5 cut(s) 27, 175, 248, 326, 372
HpyF3I CTNAG 1 cut(s) 403
HpySE526I ACGT 1 cut(s) 13
Hsp92II CATG 3 cut(s) 43, 166, 206
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 4 cut(s) 4, 268, 334, 442
LmnI GCTCC 1 cut(s) 476
LpnPI CCDG 4 cut(s) 175, 442, 448, 499
MaeI CTAG 1 cut(s) 399
MaeII ACGT 1 cut(s) 13
MaeIII GTNAC 1 cut(s) 481
MalI GATC 4 cut(s) 6, 270, 336, 444
MboI GATC 4 cut(s) 4, 268, 334, 442
MboII GAAGA 3 cut(s) 95, 402, 437
MflI RGATCY 1 cut(s) 442
MhlI GDGCHC 1 cut(s) 47
MluCI AATT 2 cut(s) 435, 532
MnlI CCTC 9 cut(s) 59, 79, 95, 136, 220, 253, 329, 433, 459
Mph1103I ATGCAT 1 cut(s) 328
MseI TTAA 1 cut(s) 537
Mva1269I GAATGC 1 cut(s) 326
MvnI CGCG 1 cut(s) 133
NcoI CCATGG 1 cut(s) 39
NdeII GATC 4 cut(s) 4, 268, 334, 442
NlaIII CATG 3 cut(s) 43, 166, 206
NlaIV GGNNCC 2 cut(s) 45, 46
NmuCI GTSAC 1 cut(s) 481
NruI TCGCGA 1 cut(s) 133
NsiI ATGCAT 1 cut(s) 328
PceI AGGCCT 1 cut(s) 318
PctI GAATGC 1 cut(s) 326
PfeI GAWTC 2 cut(s) 158, 306
Ppu21I YACGTR 1 cut(s) 14
PshAI GACNNNNGTC 1 cut(s) 488
PspN4I GGNNCC 2 cut(s) 45, 46
PspOMI GGGCCC 1 cut(s) 43
PspPI GGNCC 3 cut(s) 43, 44, 496
PsuI RGATCY 1 cut(s) 442
RruI TCGCGA 1 cut(s) 133
SaqAI TTAA 1 cut(s) 537
Sau3AI GATC 4 cut(s) 4, 268, 334, 442
Sau96I GGNCC 3 cut(s) 43, 44, 496
SduI GDGCHC 1 cut(s) 47
SetI ASST 6 cut(s) 12, 16, 154, 404, 422, 481
SinI GGWCC 1 cut(s) 496
SmlI CTYRAG 1 cut(s) 217
SmoI CTYRAG 1 cut(s) 217
SnaBI TACGTA 1 cut(s) 14
Sse9I AATT 2 cut(s) 435, 532
SseBI AGGCCT 1 cut(s) 318
SspI AATATT 2 cut(s) 169, 252
SspMI CTAG 1 cut(s) 399
StuI AGGCCT 1 cut(s) 318
StyI CCWWGG 3 cut(s) 39, 292, 499
TaiI ACGT 1 cut(s) 16
TaqI TCGA 1 cut(s) 354
TasI AATT 2 cut(s) 435, 532
TfiI GAWTC 2 cut(s) 158, 306
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TscAI CASTG 5 cut(s) 78, 120, 229, 337, 493
TseFI GTSAC 1 cut(s) 481
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 2 cut(s) 179, 209
TspRI CASTG 5 cut(s) 78, 120, 229, 337, 493
VpaK11BI GGWCC 1 cut(s) 496
XapI RAATTY 1 cut(s) 532
XcmI CCANNNNNNNNNTGG 1 cut(s) 506
XspI CTAG 1 cut(s) 399
Zsp2I ATGCAT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.