Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0009466

Physical Location & Seq

Genomic Coordinates Forward (+)

25274818 .. 25280631

Gene Structure 5814 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g22440.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3369 bp

ATGATGCCGCCGGAGCTGCAGCCGCGGCTGTTCCGCCCCTACATCTCCACCTCGGCGACAAGCGCCTCCTCTTCTTCTCTCTCCTCCTCCTTCAGCAATGGCTCCCCAAACCCTAGCCCAATCGATTCTCGCTTCAGCAACGGCCCTTCAAGATCTCTCCACAACTCGCGGTTCACCCCCGCCGCCTTCGCGCACAACGCCAGAATCGCCTTCGCGCTCGTCCCCTGCGCCGCCTTCCTCCTCGACCTTGGCGGCACTCCCGTCGCCGCAACCCTAACCCTAGGCCTCATGATCTCCTACATCGTCGACGCCCTCAACTTCAAGTCCGGTGCCTTCTTCGGCGTCTGGTTCTCCCTCGTCTTCTCCCAGATCGCCTTCTTCTTCAGCTCCTCCCTGCTGACGTCATTCAACTCATGGATGCTCGCCGGCCTCGCCGCGTTCCTCTGCGCCGAGACGAATTTCCTGATCGGCGTTTGGGTCTCTCTCCAGTTCAGGTGGATTCAAATCGAGAATCCTTCGATTGTGCTCGCCCTGGAGCGCCTCCTCTTCGCCTGTGTCCCCTTCGCCGCCTCTTCGCTCTTCACCTGGGCCACCGTCTCCGCCGTCGGTATGAACAACGCTTCCTACTACCTCATGGCCTTCAGCTGCATTTTCTATTGGCTCTACTCCATTCCTCGCATTTCGTCTTTCAAGACCAAGCAGGACTCCAAGTACCACGGCGGCGAGGTCCCCGACGAGAATCTCATCCTCAGCCCCCTGGAAAGCTGCATCCACACTCTGTATCTGCTCTTCTTCCCGCTGCTCTTCCACATTGCCTCGCACTACTCCATCATGTTCTCATCAGCCACCGCGGTTTCGGATTTGTTCCTGCTCTTCTTCGTTCCGTTCTTGTTCCAGCTCCTTGCATCCACCAGGGGAGCACTCTGGTGGGTCACGAAGAATCCGAGCCAGCTGCGGGGGATTCAAGTCATGAATGGCGCCATTGCTTTGGTTGTTGTTGTGATCTGTTTGGAGATTAGAGTTATTTTCCATTCCTTTGGGCGGTACATTCAGGTGCCTCCGCCGCTGAATTACCTTTTGGTGACTACTACTATGCTTGGAGGGGCAGCTGGAGCTGGTGCGTACGCCCTTGGTGTGATATCTGATGCTTTCAGCTCCCTGGCTTTCACTGCTCTGGCTGTTGTTGTTAGTGCAGCTGGAGCTATCGTTGTCGGATTTCCTGTTTTGTTCCTTCCACTGCCTGCAGTTGCTGGCTTCTATTTGGCTCGCTTTTTTACAAAGAAGAGTATTCCATCATACTTTGCTTTTGTTGTGCTTGGGAGCTTGATGGTTACATGGTTCGTTATGCATAATTTCTGGGATCTAAATATTTGGATGGCTGGCATGTCCCTGAAGTCTTTCTGCAAACTTGTAATTTTGAATGTTGTCTTGGCCTTGACTATTCCTGGTCTAGCTCTACTTCCTTCGAAACTCCACTTTTTGACAGAGATTGGTTTAGTTGGCCATGCATTGCTTATTTCTCACCTGGAGAACCGATTTTTTAATTACTCTGGCATGTACTATTATGGGTTTGAGGATGATGTAATGTATCCAAGCTACATGGTTCTCGTGACAACATTTGTGGGTTTAGCTTTAGTGAGAAGACTGTCTGCAGATAATCGAATTGGTGCAAAGGCAGTTTGGATTTTGAATTGTCTTTATTCTGCAAAGCTGGGTATGCTAGTAATTTCATCAAAGTCTGTTGTATGGATGTCAGCTGTCCTGTTATTGGCTGTAACACCACCATTGCTTCTCTACAAGGATAAGTCAAGAACAGCCTCAAAGATGCAAACATGGCAAGGTTATGCACATGCAGGTGTGGTATCTTTATCAGTTTGGTTTTGTCGTGAAACAATCTTTGAAGCCCTGCAGTGGTGGAATGGAAGAGCTCCCTCTGATGGCTTACTCTTGGGCTCCTGTATTGTCTTGATGGGCTTGGCTTGTATTCCTATAGTGGCTCTCCACTTCTCTCATGTCTTGCCTGCCAAAAGATGCTTAGTGCTGGTGGTGGCTACTGGTTTGCTGTTTATCCTTATGCAACCACCTATCCCAGTATCATGGACTTACCGGTCTGACCTAATTAAAGCTGCCCGGCAGTCTGTTGATGACGTCTCCATATATGGGTTCATTGCACCAAAGCCTATGTGGCCATCATGGCTGCTAATTGTTGCTATCCTGCTTACTCTAGCTGCTGTTACATCTGTGATACCAATCAAATACATGGTTGAATTGAGAGTGTTTTACTCCATAGCCATGGGACTTGCTCTAGGTATCTACATATCTACCGAGTTCTTCCTTCAGGCAGCTGTCCTGCATGTCCTCATTGTTGTGACCATGGTTTGTACCTCTGTGTTTGTGGTTTTCACTCATTTCCCATCTGCCTCAAGTACGAAGCTGCTTCCATGGATTTTTGCTTTACTAGTAGCTCTCTTTCCTGTGACATATCTTTTGGAGGGCCAAGTGAGAATCAAAAGCATGCTTGGGGATGGTGGATTTGGAGATCTGGGAGAAGAAGAGAGGAAGCTTACAACTCTATTTGCTGTTGAGGGGGCAAGGACATCTCTTCTTGGTCTTTATGCAGCGATTTTCATGCTAGTAGCCCTAGAGGTAAAGTATGAACTTGCCTCATTATTGAGGGAGAAGGCTACTGAAAGAAGTGGAATTAGACATAGTCTTTCTGGTCAAAGCACATCTACTAGCTTTCCGTCAAGAATGAGATTCATGCAACAGCGCCGGGCTTCTAGTATCTCATCCTTCACAATCAAGAAAATGACTGCTGAGGGAGCTTGGATGCCAGCAGTTGGTAATGTTGCTACTGTGATGTGCTTTGCCATATGCATCATCTTAAATGTCAATCTCACAGGTGGCTCAAATCGTGCCATATTTTTCCTGGCACCAATTTTGCTGCTACTCAACCAGGATTCAGATTTTGTTGCTGGGTTTGGGGACAAGCAGAGGTATTTCCCTGTTACAGTAGTTATATCATCCTACCTAGTCATCACTGCTGTGTACAGCATATGGGAAGAAATCTGGCACGGGAATGTTGGTTGGGGAATGGAGATTGGTGGGCCGGATTGGTTCTTTGCGGTGAAGAATTTGGCGCTCCTTATTCTTACATTCCCGAGCCATATCCTTTTCAACAGGTACGTGTGGAGTTTAACAAAGCAGACAGACTCAACTCCATTGATAACTATGCCCCTCAATCTGCCATCTGTAATAATAACAGATGTTCTGAAGGTTAGGATATTAGGTCTCTTAGGAATTATTTATTCCCTAGCCCAGTATCTAGTATCTAGACAACAGTACATCTCAGGATTGAAGTATATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1123

Amino Acids

123.73

Weight (kDa)

9.2

Isoelectric Point (pI)

40.59

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009466)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13390 AT5G13390
fragaria_vesca	FvH4_4g22440
malus_domestica	MD13G1132200.v1.1 MD16G1142500.v1.1
prunus_persica	Prupe.1G213300_v2.0.a1
pyrus_communis	pycom13g11500 pycom16g11290
rosa_chinensis	RchiOBHm_Chr4g0402251 RchiOBHm_Chr4g0428701
rosa_laevigata	RLG00000007134
rosa_multiflora	Rmu_sc0003316.1_g000007
rosa_roxburghii	Rroxscaffold_5G00370370
rosa_rugosa	Rorug04G0227300
rosa_samantha	Rh4AG284500 Rh4BG290500 Rh4CG306700 Rh4DG288100 Rh5DG506600
rosa_wichuraiana	Rw0G020630 Rw4G015480 Rw4G024710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1844
AasI	GACNNNNNNGTC	1 cut(s)	2107
AatII	GACGTC	2 cut(s)	404, 2151
Acc36I	ACCTGC	1 cut(s)	1844
AccB1I	GGYRCC	4 cut(s)	329, 977, 1054, 2932
AccB7I	CCANNNNNTGG	1 cut(s)	2840
AccI	GTMKAC	1 cut(s)	306
AccII	CGCG	6 cut(s)	25, 169, 191, 215, 437, 851
AclWI	GGATC	1 cut(s)	1368
AcoI	YGGCCR	2 cut(s)	1501, 2186
AcsI	RAATTY	2 cut(s)	457, 3133
AcuI	CTGAAG	7 cut(s)	76, 118, 367, 625, 1412, 2321, 3293
AcyI	GRCGYC	5 cut(s)	309, 342, 401, 978, 2148
AfaI	GTAC	9 cut(s)	713, 1046, 1124, 1559, 2383, 2428, 3050, 3185, 3344
AfiI	CCNNNNNNNGG	7 cut(s)	955, 1041, 1768, 1911, 1937, 2160, 2840
AflIII	ACRYGT	1 cut(s)	3186
AgeI	ACCGGT	1 cut(s)	2106
AhlI	ACTAGT	1 cut(s)	2458
AjnI	CCWGG	9 cut(s)	531, 584, 756, 911, 1158, 1444, 1524, 2928, 2955
AjuI	GAANNNNNNNTTGG	4 cut(s)	1061, 1093, 1412, 1444
AleI	CACNNNNGTG	2 cut(s)	925, 3044
AloI	GAACNNNNNNTCC	2 cut(s)	605, 637
Alw21I	GWGCWC	3 cut(s)	528, 922, 1930
Alw26I	GTCTC	5 cut(s)	446, 484, 601, 2155, 3296
AlwI	GGATC	1 cut(s)	1368
AlwNI	CAGNNNCTG	1 cut(s)	1250
Ama87I	CYCGRG	1 cut(s)	3160
ApoI	RAATTY	2 cut(s)	457, 3133
ArsI	GACNNNNNNTTYG	2 cut(s)	2470, 2502
AsiGI	ACCGGT	1 cut(s)	2106
Asp700I	GAANNNNTTC	1 cut(s)	1397
AspA2I	CCTAGG	1 cut(s)	280
AspLEI	GCGC	9 cut(s)	65, 193, 217, 230, 449, 540, 980, 2772, 3142
AspS9I	GGNCC	5 cut(s)	143, 588, 727, 2494, 3107
AsuC2I	CCSGG	2 cut(s)	2131, 2774
AsuHPI	GGTGA	5 cut(s)	166, 574, 1093, 1514, 3139
AsuII	TTCGAA	1 cut(s)	1466
AvaI	CYCGRG	1 cut(s)	3160
AvaII	GGWCC	1 cut(s)	727
AvrII	CCTAGG	1 cut(s)	280
BalI	TGGCCA	2 cut(s)	1503, 2188
BanI	GGYRCC	4 cut(s)	329, 977, 1054, 2932
BanII	GRGCYC	2 cut(s)	1930, 1955
BarI	GAAGNNNNNNTAC	2 cut(s)	773, 805
BauI	CACGAG	1 cut(s)	1607
BbsI	GAAGAC	2 cut(s)	352, 1648
Bbv12I	GWGCWC	3 cut(s)	528, 922, 1930
BbvCI	CCTCAGC	2 cut(s)	749, 2817
BceAI	ACGGC	3 cut(s)	157, 587, 733
BcgI	CGANNNNNNTGC	6 cut(s)	244, 278, 934, 968, 2611, 2645
BciT130I	CCWGG	9 cut(s)	533, 586, 758, 913, 1160, 1446, 1526, 2930, 2957
BciVI	GTATCC	1 cut(s)	1599
BcnI	CCSGG	2 cut(s)	2131, 2774
BcoDI	GTCTC	5 cut(s)	446, 484, 601, 2155, 3296
BcuI	ACTAGT	1 cut(s)	2458
BfmI	CTRYAG	5 cut(s)	17, 1242, 1650, 1907, 1989
BfoI	RGCGCY	5 cut(s)	66, 541, 981, 2773, 3143
BfuAI	ACCTGC	1 cut(s)	1844
BfuI	GTATCC	1 cut(s)	1599
BglI	GCCNNNNNGGC	2 cut(s)	2185, 2194
BglII	AGATCT	2 cut(s)	152, 2539
BlnI	CCTAGG	1 cut(s)	280
Bme18I	GGWCC	1 cut(s)	727
BmeT110I	CYCGRG	1 cut(s)	3160
BmgT120I	GGNCC	5 cut(s)	143, 588, 727, 2494, 3107
BmiI	GGNNCC	7 cut(s)	103, 331, 729, 979, 1056, 1954, 2934
BmrI	ACTGGG	2 cut(s)	2084, 3313
BmsI	GCATC	8 cut(s)	408, 777, 914, 1135, 1815, 2021, 2820, 2886
BmuI	ACTGGG	2 cut(s)	2084, 3313
BpiI	GAAGAC	2 cut(s)	352, 1648
BpmI	CTGGAG	5 cut(s)	470, 554, 1131, 1218, 1547
Bpu10I	CCTNAGC	2 cut(s)	749, 2817
Bpu14I	TTCGAA	1 cut(s)	1466
BpuEI	CTTGAG	1 cut(s)	2407
BpuMI	CCSGG	2 cut(s)	2131, 2774
Bsa29I	ATCGAT	1 cut(s)	123
BsaAI	YACGTR	1 cut(s)	3187
BsaBI	GATNNNNATC	2 cut(s)	503, 2249
BsaHI	GRCGYC	5 cut(s)	309, 342, 401, 978, 2148
BsaI	GGTCTC	2 cut(s)	484, 3296
BsaWI	WCCGGW	2 cut(s)	326, 2106
BsaXI	ACNNNNNCTCC	2 cut(s)	1191, 1221
Bsc4I	CCNNNNNNNGG	7 cut(s)	955, 1041, 1768, 1911, 1937, 2160, 2840
Bse118I	RCCGGY	2 cut(s)	425, 2106
Bse1I	ACTGG	4 cut(s)	487, 2059, 2090, 3319
Bse3DI	GCAATG	6 cut(s)	103, 810, 981, 1508, 1784, 2166
Bse8I	GATNNNNATC	2 cut(s)	503, 2249
BseBI	CCWGG	9 cut(s)	533, 586, 758, 913, 1160, 1446, 1526, 2930, 2957
BseCI	ATCGAT	1 cut(s)	123
BseJI	GATNNNNATC	2 cut(s)	503, 2249
BseLI	CCNNNNNNNGG	7 cut(s)	955, 1041, 1768, 1911, 1937, 2160, 2840
BseMI	GCAATG	6 cut(s)	103, 810, 981, 1508, 1784, 2166
BseMII	CTCAG	3 cut(s)	763, 2808, 3363
BseNI	ACTGG	4 cut(s)	487, 2059, 2090, 3319
BseRI	GAGGAG	6 cut(s)	58, 73, 76, 230, 379, 533
BseYI	CCCAGC	2 cut(s)	1711, 2975
BsgI	GTGCAG	1 cut(s)	1212
Bsh1236I	CGCG	6 cut(s)	25, 169, 191, 215, 437, 851
BshNI	GGYRCC	4 cut(s)	329, 977, 1054, 2932
BshTI	ACCGGT	1 cut(s)	2106
BshVI	ATCGAT	1 cut(s)	123
BsiHKAI	GWGCWC	3 cut(s)	528, 922, 1930
BsiHKCI	CYCGRG	1 cut(s)	3160
BsiSI	CCGG	7 cut(s)	11, 327, 426, 2107, 2131, 2773, 3110
BsiWI	CGTACG	1 cut(s)	1122
BslFI	GGGAC	6 cut(s)	206, 542, 713, 1372, 2310, 2999
BslI	CCNNNNNNNGG	7 cut(s)	955, 1041, 1768, 1911, 1937, 2160, 2840
BsmAI	GTCTC	5 cut(s)	446, 484, 601, 2155, 3296
BsmBI	CGTCTC	3 cut(s)	446, 601, 2155
BsmFI	GGGAC	6 cut(s)	206, 542, 713, 1372, 2310, 2999
Bso31I	GGTCTC	2 cut(s)	484, 3296
BsoBI	CYCGRG	1 cut(s)	3160
Bsp119I	TTCGAA	1 cut(s)	1466
Bsp1286I	GDGCHC	4 cut(s)	528, 922, 1930, 1955
Bsp1407I	TGTACA	1 cut(s)	3048
Bsp143I	GATC	7 cut(s)	152, 291, 369, 465, 1002, 1360, 2539
Bsp19I	CCATGG	3 cut(s)	2292, 2373, 2441
BspCNI	CTCAG	3 cut(s)	762, 2809, 3362
BspDI	ATCGAT	1 cut(s)	123
BspFNI	CGCG	6 cut(s)	25, 169, 191, 215, 437, 851
BspHI	TCATGA	2 cut(s)	288, 969
BspLI	GGNNCC	7 cut(s)	103, 331, 729, 979, 1056, 1954, 2934
BspMAI	CTGCAG	4 cut(s)	21, 1246, 1654, 1911
BspMI	ACCTGC	1 cut(s)	1844
BspPI	GGATC	1 cut(s)	1368
BspQI	GCTCTTC	5 cut(s)	584, 794, 809, 878, 1918
BspT104I	TTCGAA	1 cut(s)	1466
BspT107I	GGYRCC	4 cut(s)	329, 977, 1054, 2932
BspTNI	GGTCTC	2 cut(s)	484, 3296
BsrDI	GCAATG	6 cut(s)	103, 810, 981, 1508, 1784, 2166
BsrFI	RCCGGY	2 cut(s)	425, 2106
BsrGI	TGTACA	1 cut(s)	3048
BsrI	ACTGG	4 cut(s)	487, 2059, 2090, 3319
BssAI	RCCGGY	2 cut(s)	425, 2106
BssMI	GATC	7 cut(s)	152, 291, 369, 465, 1002, 1360, 2539
BssNI	GRCGYC	5 cut(s)	309, 342, 401, 978, 2148
BssSI	CACGAG	1 cut(s)	1607
BssT1I	CCWWGG	6 cut(s)	247, 280, 1129, 2292, 2373, 2441
Bst2BI	CACGAG	1 cut(s)	1607
Bst2UI	CCWGG	9 cut(s)	533, 586, 758, 913, 1160, 1446, 1526, 2930, 2957
Bst4CI	ACNGT	5 cut(s)	595, 1647, 2857, 3013, 3342
BstACI	GRCGYC	5 cut(s)	309, 342, 401, 978, 2148
BstAUI	TGTACA	1 cut(s)	3048
BstBAI	YACGTR	1 cut(s)	3187
BstBI	TTCGAA	1 cut(s)	1466
BstDEI	CTNAG	5 cut(s)	749, 2035, 2817, 3295, 3349
BstDSI	CCRYGG	6 cut(s)	23, 715, 849, 2292, 2373, 2441
BstFNI	CGCG	6 cut(s)	25, 169, 191, 215, 437, 851
BstH2I	RGCGCY	5 cut(s)	66, 541, 981, 2773, 3143
BstHHI	GCGC	9 cut(s)	65, 193, 217, 230, 449, 540, 980, 2772, 3142
BstKTI	GATC	7 cut(s)	155, 294, 372, 468, 1005, 1363, 2542
BstMAI	GTCTC	5 cut(s)	446, 484, 601, 2155, 3296
BstMBI	GATC	7 cut(s)	152, 291, 369, 465, 1002, 1360, 2539
BstNI	CCWGG	9 cut(s)	533, 586, 758, 913, 1160, 1446, 1526, 2930, 2957
BstNSI	RCATGY	5 cut(s)	1387, 1558, 1853, 2357, 2518
BstSFI	CTRYAG	5 cut(s)	17, 1242, 1650, 1907, 1989
BstUI	CGCG	6 cut(s)	25, 169, 191, 215, 437, 851
BstV2I	GAAGAC	2 cut(s)	352, 1648
BstX2I	RGATCY	3 cut(s)	152, 1360, 2539
BstXI	CCANNNNNNTGG	4 cut(s)	988, 1037, 2097, 2293
BstYI	RGATCY	3 cut(s)	152, 1360, 2539
Bsu15I	ATCGAT	1 cut(s)	123
BsuI	GTATCC	1 cut(s)	1599
BsuTUI	ATCGAT	1 cut(s)	123
BtgI	CCRYGG	6 cut(s)	23, 715, 849, 2292, 2373, 2441
BtsI	GCAGTG	4 cut(s)	1167, 1235, 1916, 3039
BtsIMutI	CAGTG	4 cut(s)	1167, 1235, 1916, 3039
BveI	ACCTGC	1 cut(s)	1844
CaiI	CAGNNNCTG	1 cut(s)	1250
CciI	TCATGA	2 cut(s)	288, 969
CfoI	GCGC	9 cut(s)	65, 193, 217, 230, 449, 540, 980, 2772, 3142
Cfr10I	RCCGGY	2 cut(s)	425, 2106
Cfr13I	GGNCC	5 cut(s)	143, 588, 727, 2494, 3107
Cfr42I	CCGCGG	2 cut(s)	26, 852
ClaI	ATCGAT	1 cut(s)	123
CseI	GACGC	2 cut(s)	317, 331
Csp6I	GTAC	9 cut(s)	712, 1045, 1123, 1558, 2382, 2427, 3049, 3184, 3343
CspAI	ACCGGT	1 cut(s)	2106
CspCI	CAANNNNNGTGG	2 cut(s)	1602, 1637
CviQI	GTAC	9 cut(s)	712, 1045, 1123, 1558, 2382, 2427, 3049, 3184, 3343
DdeI	CTNAG	5 cut(s)	749, 2035, 2817, 3295, 3349
DinI	GGCGCC	1 cut(s)	979
DpnI	GATC	7 cut(s)	154, 293, 371, 467, 1004, 1362, 2541
DpnII	GATC	7 cut(s)	152, 291, 369, 465, 1002, 1360, 2539
DrdI	GACNNNNNNGTC	1 cut(s)	2107
DseDI	GACNNNNNNGTC	1 cut(s)	2107
EaeI	YGGCCR	2 cut(s)	1501, 2186
EciI	GGCGGA	3 cut(s)	23, 589, 1050
Ecl136II	GAGCTC	1 cut(s)	1928
Eco130I	CCWWGG	6 cut(s)	247, 280, 1129, 2292, 2373, 2441
Eco147I	AGGCCT	1 cut(s)	285
Eco24I	GRGCYC	2 cut(s)	1930, 1955
Eco31I	GGTCTC	2 cut(s)	484, 3296
Eco32I	GATATC	1 cut(s)	1140
Eco47I	GGWCC	1 cut(s)	727
Eco53kI	GAGCTC	1 cut(s)	1928
Eco57I	CTGAAG	7 cut(s)	76, 118, 367, 625, 1412, 2321, 3293
Eco88I	CYCGRG	1 cut(s)	3160
EcoICRI	GAGCTC	1 cut(s)	1928
EcoO109I	RGGNCCY	1 cut(s)	727
EcoRII	CCWGG	9 cut(s)	531, 584, 756, 911, 1158, 1444, 1524, 2928, 2955
EcoRV	GATATC	1 cut(s)	1140
EcoT14I	CCWWGG	6 cut(s)	247, 280, 1129, 2292, 2373, 2441
EcoT22I	ATGCAT	3 cut(s)	1350, 1510, 2879
EcoT38I	GRGCYC	2 cut(s)	1930, 1955
EgeI	GGCGCC	1 cut(s)	979
EheI	GGCGCC	1 cut(s)	979
ErhI	CCWWGG	6 cut(s)	247, 280, 1129, 2292, 2373, 2441
Esp3I	CGTCTC	3 cut(s)	446, 601, 2155
FaqI	GGGAC	6 cut(s)	206, 542, 713, 1372, 2310, 2999
FauI	CCCGC	3 cut(s)	187, 804, 948
FauNDI	CATATG	2 cut(s)	2873, 3056
FblI	GTMKAC	1 cut(s)	306
FriOI	GRGCYC	2 cut(s)	1930, 1955
GlaI	GCGC	9 cut(s)	64, 192, 216, 229, 448, 539, 979, 2771, 3141
GsaI	CCCAGC	2 cut(s)	1715, 2979
GsuI	CTGGAG	5 cut(s)	470, 554, 1131, 1218, 1547
HaeII	RGCGCY	5 cut(s)	66, 541, 981, 2773, 3143
HapII	CCGG	7 cut(s)	11, 327, 426, 2107, 2131, 2773, 3110
HgaI	GACGC	2 cut(s)	317, 331
HhaI	GCGC	9 cut(s)	65, 193, 217, 230, 449, 540, 980, 2772, 3142
Hin1I	GRCGYC	5 cut(s)	309, 342, 401, 978, 2148
Hin6I	GCGC	9 cut(s)	63, 191, 215, 228, 447, 538, 978, 2770, 3140
HinP1I	GCGC	9 cut(s)	63, 191, 215, 228, 447, 538, 978, 2770, 3140
HincII	GTYRAC	1 cut(s)	307
HindII	GTYRAC	1 cut(s)	307
HindIII	AAGCTT	1 cut(s)	2561
HpaII	CCGG	7 cut(s)	11, 327, 426, 2107, 2131, 2773, 3110
HphI	GGTGA	5 cut(s)	166, 574, 1093, 1514, 3139
Hpy166II	GTNNAC	3 cut(s)	174, 307, 3049
Hpy188I	TCNGA	8 cut(s)	859, 945, 1144, 1214, 1936, 2113, 2965, 3273
Hpy8I	GTNNAC	3 cut(s)	174, 307, 3049
Hpy99I	CGWCG	5 cut(s)	266, 308, 311, 608, 737
HpyCH4III	ACNGT	5 cut(s)	595, 1647, 2857, 3013, 3342
HpyCH4IV	ACGT	3 cut(s)	401, 2148, 3186
HpyF3I	CTNAG	5 cut(s)	749, 2035, 2817, 3295, 3349
HpySE526I	ACGT	3 cut(s)	401, 2148, 3186
Hsp92I	GRCGYC	5 cut(s)	309, 342, 401, 978, 2148
HspAI	GCGC	9 cut(s)	63, 191, 215, 228, 447, 538, 978, 2770, 3140
KasI	GGCGCC	1 cut(s)	977
KroI	GCCGGC	1 cut(s)	425
KroNI	GCCGGC	1 cut(s)	427
KspI	CCGCGG	2 cut(s)	26, 852
Kzo9I	GATC	7 cut(s)	152, 291, 369, 465, 1002, 1360, 2539
LguI	GCTCTTC	5 cut(s)	584, 794, 809, 878, 1918
LweI	GCATC	8 cut(s)	408, 777, 914, 1135, 1815, 2021, 2820, 2886
MaeII	ACGT	3 cut(s)	401, 2148, 3186
MaeIII	GTNAC	9 cut(s)	931, 1081, 1330, 1609, 1774, 2233, 2368, 2476, 3007
MalI	GATC	7 cut(s)	154, 293, 371, 467, 1004, 1362, 2541
MboI	GATC	7 cut(s)	152, 291, 369, 465, 1002, 1360, 2539
MflI	RGATCY	3 cut(s)	152, 1360, 2539
MhlI	GDGCHC	4 cut(s)	528, 922, 1930, 1955
MlsI	TGGCCA	2 cut(s)	1503, 2188
MluNI	TGGCCA	2 cut(s)	1503, 2188
Mly113I	GGCGCC	1 cut(s)	978
MlyI	GAGTC	2 cut(s)	698, 3206
MmeI	TCCRAC	1 cut(s)	1192
Mox20I	TGGCCA	2 cut(s)	1503, 2188
Mph1103I	ATGCAT	3 cut(s)	1350, 1510, 2879
MroNI	GCCGGC	1 cut(s)	425
MroXI	GAANNNNTTC	1 cut(s)	1397
MscI	TGGCCA	2 cut(s)	1503, 2188
MseI	TTAA	4 cut(s)	1542, 2121, 2885, 3197
MslI	CAYNNNNRTG	7 cut(s)	925, 1052, 1854, 2291, 2366, 3044, 3078
Msp20I	TGGCCA	2 cut(s)	1503, 2188
MspI	CCGG	7 cut(s)	11, 327, 426, 2107, 2131, 2773, 3110
MvaI	CCWGG	9 cut(s)	533, 586, 758, 913, 1160, 1446, 1526, 2930, 2957
MvnI	CGCG	6 cut(s)	25, 169, 191, 215, 437, 851
NaeI	GCCGGC	1 cut(s)	427
NarI	GGCGCC	1 cut(s)	978
NciI	CCSGG	2 cut(s)	2131, 2774
NcoI	CCATGG	3 cut(s)	2292, 2373, 2441
NdeI	CATATG	2 cut(s)	2873, 3056
NdeII	GATC	7 cut(s)	152, 291, 369, 465, 1002, 1360, 2539
NgoMIV	GCCGGC	1 cut(s)	425
NlaIV	GGNNCC	7 cut(s)	103, 331, 729, 979, 1056, 1954, 2934
NmeAIII	GCCGAG	2 cut(s)	32, 475
NmuCI	GTSAC	5 cut(s)	931, 1081, 1609, 2368, 2476
NsiI	ATGCAT	3 cut(s)	1350, 1510, 2879
NspI	RCATGY	5 cut(s)	1387, 1558, 1853, 2357, 2518
NspV	TTCGAA	1 cut(s)	1466
OliI	CACNNNNGTG	2 cut(s)	925, 3044
PaeI	GCATGC	1 cut(s)	2518
PagI	TCATGA	2 cut(s)	288, 969
PaqCI	CACCTGC	1 cut(s)	1844
PceI	AGGCCT	1 cut(s)	285
PciSI	GCTCTTC	5 cut(s)	584, 794, 809, 878, 1918
PcsI	WCGNNNNNNNCGW	1 cut(s)	941
PdiI	GCCGGC	1 cut(s)	427
PdmI	GAANNNNTTC	1 cut(s)	1397
Pfl23II	CGTACG	1 cut(s)	1122
PflFI	GACNNNGTC	1 cut(s)	2709
PflMI	CCANNNNNTGG	1 cut(s)	2840
PinAI	ACCGGT	1 cut(s)	2106
PleI	GAGTC	2 cut(s)	698, 3206
PluTI	GGCGCC	1 cut(s)	981
PpsI	GAGTC	2 cut(s)	698, 3206
Ppu21I	YACGTR	1 cut(s)	3187
PpuMI	RGGWCCY	1 cut(s)	727
Psp124BI	GAGCTC	1 cut(s)	1930
Psp5II	RGGWCCY	1 cut(s)	727
Psp6I	CCWGG	9 cut(s)	531, 584, 756, 911, 1158, 1444, 1524, 2928, 2955
PspFI	CCCAGC	2 cut(s)	1711, 2975
PspGI	CCWGG	9 cut(s)	531, 584, 756, 911, 1158, 1444, 1524, 2928, 2955
PspLI	CGTACG	1 cut(s)	1122
PspN4I	GGNNCC	7 cut(s)	103, 331, 729, 979, 1056, 1954, 2934
PspPI	GGNCC	5 cut(s)	143, 588, 727, 2494, 3107
PspPPI	RGGWCCY	1 cut(s)	727
PstI	CTGCAG	4 cut(s)	21, 1246, 1654, 1911
PstNI	CAGNNNCTG	1 cut(s)	1250
PsuI	RGATCY	3 cut(s)	152, 1360, 2539
PsyI	GACNNNGTC	1 cut(s)	2709
PvuII	CAGCTG	6 cut(s)	645, 952, 1109, 1196, 1757, 2345
RsaI	GTAC	9 cut(s)	713, 1046, 1124, 1559, 2383, 2428, 3050, 3185, 3344
RsaNI	GTAC	9 cut(s)	712, 1045, 1123, 1558, 2382, 2427, 3049, 3184, 3343
RseI	CAYNNNNRTG	7 cut(s)	925, 1052, 1854, 2291, 2366, 3044, 3078
SacI	GAGCTC	1 cut(s)	1930
SacII	CCGCGG	2 cut(s)	26, 852
SalI	GTCGAC	1 cut(s)	305
SapI	GCTCTTC	5 cut(s)	584, 794, 809, 878, 1918
SaqAI	TTAA	4 cut(s)	1542, 2121, 2885, 3197
Sau3AI	GATC	7 cut(s)	152, 291, 369, 465, 1002, 1360, 2539
Sau96I	GGNCC	5 cut(s)	143, 588, 727, 2494, 3107
SchI	GAGTC	2 cut(s)	698, 3206
SduI	GDGCHC	4 cut(s)	528, 922, 1930, 1955
SfaNI	GCATC	8 cut(s)	408, 777, 914, 1135, 1815, 2021, 2820, 2886
SfcI	CTRYAG	5 cut(s)	17, 1242, 1650, 1907, 1989
SfoI	GGCGCC	1 cut(s)	979
Sfr303I	CCGCGG	2 cut(s)	26, 852
SfuI	TTCGAA	1 cut(s)	1466
SgrBI	CCGCGG	2 cut(s)	26, 852
SgrDI	CGTCGACG	1 cut(s)	305
SinI	GGWCC	1 cut(s)	727
SmiMI	CAYNNNNRTG	7 cut(s)	925, 1052, 1854, 2291, 2366, 3044, 3078
SmlI	CTYRAG	1 cut(s)	2422
SmoI	CTYRAG	1 cut(s)	2422
SpeI	ACTAGT	1 cut(s)	2458
SphI	GCATGC	1 cut(s)	2518
SseBI	AGGCCT	1 cut(s)	285
SspDI	GGCGCC	1 cut(s)	977
SspI	AATATT	1 cut(s)	1369
SstI	GAGCTC	1 cut(s)	1930
StuI	AGGCCT	1 cut(s)	285
StyI	CCWWGG	6 cut(s)	247, 280, 1129, 2292, 2373, 2441
TaaI	ACNGT	5 cut(s)	595, 1647, 2857, 3013, 3342
TaiI	ACGT	3 cut(s)	404, 2151, 3189
TaqI	TCGA	7 cut(s)	123, 243, 306, 507, 518, 1466, 1660
TatI	WGTACW	3 cut(s)	1557, 3048, 3342
Tru1I	TTAA	4 cut(s)	1542, 2121, 2885, 3197
Tru9I	TTAA	4 cut(s)	1542, 2121, 2885, 3197
TscAI	CASTG	4 cut(s)	1174, 1242, 1916, 3046
TseFI	GTSAC	5 cut(s)	931, 1081, 1609, 2368, 2476
Tsp45I	GTSAC	5 cut(s)	931, 1081, 1609, 2368, 2476
TspDTI	ATGAA	7 cut(s)	626, 986, 1719, 2155, 2617, 2670, 2749
TspGWI	ACGGA	2 cut(s)	873, 2733
TspRI	CASTG	4 cut(s)	1174, 1242, 1916, 3046
Tth111I	GACNNNGTC	1 cut(s)	2709
Van91I	CCANNNNNTGG	1 cut(s)	2840
VpaK11BI	GGWCC	1 cut(s)	727
XapI	RAATTY	2 cut(s)	457, 3133
XbaI	TCTAGA	1 cut(s)	3332
XceI	RCATGY	5 cut(s)	1387, 1558, 1853, 2357, 2518
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2181, 2926
XmaJI	CCTAGG	1 cut(s)	280
XmiI	GTMKAC	1 cut(s)	306
XmnI	GAANNNNTTC	1 cut(s)	1397
ZraI	GACGTC	2 cut(s)	402, 2149
Zsp2I	ATGCAT	3 cut(s)	1350, 1510, 2879

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.