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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G13390 AT5G13390 |
| fragaria_vesca | FvH4_4g22440 |
| malus_domestica | MD13G1132200.v1.1 MD16G1142500.v1.1 |
| prunus_persica | Prupe.1G213300_v2.0.a1 |
| pyrus_communis | pycom13g11500 pycom16g11290 |
| rosa_chinensis | RchiOBHm_Chr4g0402251 RchiOBHm_Chr4g0428701 |
| rosa_laevigata | RLG00000007134 |
| rosa_multiflora | Rmu_sc0003316.1_g000007 |
| rosa_roxburghii | Rroxscaffold_5G00370370 |
| rosa_rugosa | Rorug04G0227300 |
| rosa_samantha | Rh4AG284500 Rh4BG290500 Rh4CG306700 Rh4DG288100 Rh5DG506600 |
| rosa_wichuraiana | Rw0G020630 Rw4G015480 Rw4G024710 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1835 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 2098 |
| Acc36I | ACCTGC | 1 cut(s) | 1835 |
| AccB1I | GGYRCC | 3 cut(s) | 320, 1045, 2923 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1928, 2831 |
| AccBSI | CCGCTC | 1 cut(s) | 1106 |
| AccI | GTMKAC | 2 cut(s) | 297, 2304 |
| AccII | CGCG | 4 cut(s) | 25, 521, 911, 948 |
| AclWI | GGATC | 2 cut(s) | 1359, 3116 |
| AcoI | YGGCCR | 2 cut(s) | 1492, 2177 |
| AcsI | RAATTY | 1 cut(s) | 3124 |
| AcuI | CTGAAG | 5 cut(s) | 358, 1218, 1403, 2312, 3284 |
| AcyI | GRCGYC | 3 cut(s) | 300, 333, 462 |
| AfeI | AGCGCT | 1 cut(s) | 2631 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 752, 1238, 1928, 2032, 2831 |
| AgeI | ACCGGT | 1 cut(s) | 2097 |
| AhlI | ACTAGT | 1 cut(s) | 2449 |
| AjiI | CACGTC | 1 cut(s) | 3259 |
| AjnI | CCWGG | 5 cut(s) | 575, 1435, 2686, 2946, 3303 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1052, 1084, 1403, 1435 |
| AleI | CACNNNNGTG | 1 cut(s) | 711 |
| Alw21I | GWGCWC | 1 cut(s) | 62 |
| Alw26I | GTCTC | 7 cut(s) | 281, 289, 437, 475, 2374, 2523, 2782 |
| AlwI | GGATC | 2 cut(s) | 1359, 3116 |
| Ama87I | CYCGRG | 3 cut(s) | 524, 2413, 3151 |
| Aor51HI | AGCGCT | 1 cut(s) | 2631 |
| AoxI | GGCC | 8 cut(s) | 579, 1492, 2177, 2485, 2684, 3015, 3129, 3306 |
| ApoI | RAATTY | 1 cut(s) | 3124 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 595, 627, 2461, 2493, 2942, 2974 |
| AseI | ATTAAT | 1 cut(s) | 2660 |
| AsiGI | ACCGGT | 1 cut(s) | 2097 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1388 |
| AspLEI | GCGC | 8 cut(s) | 221, 440, 523, 531, 911, 913, 948, 2632 |
| AspS9I | GGNCC | 7 cut(s) | 134, 579, 718, 2485, 3098, 3130, 3307 |
| AvaI | CYCGRG | 3 cut(s) | 524, 2413, 3151 |
| AvaII | GGWCC | 3 cut(s) | 134, 718, 3098 |
| AxyI | CCTNAGG | 1 cut(s) | 271 |
| BaeGI | GKGCMC | 1 cut(s) | 1050 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 237, 270 |
| BalI | TGGCCA | 1 cut(s) | 1494 |
| BanI | GGYRCC | 3 cut(s) | 320, 1045, 2923 |
| BanII | GRGCYC | 1 cut(s) | 3285 |
| BbsI | GAAGAC | 2 cut(s) | 343, 1921 |
| Bbv12I | GWGCWC | 1 cut(s) | 62 |
| BbvCI | CCTCAGC | 1 cut(s) | 2808 |
| BccI | CCATC | 9 cut(s) | 593, 1312, 1360, 1468, 1922, 1930, 2413, 3044, 3247 |
| BceAI | ACGGC | 8 cut(s) | 94, 185, 578, 724, 1039, 1860, 2164, 3196 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 535, 569, 925, 959 |
| BciT130I | CCWGG | 5 cut(s) | 577, 1437, 2688, 2948, 3305 |
| BcoDI | GTCTC | 7 cut(s) | 281, 289, 437, 475, 2374, 2523, 2782 |
| BcuI | ACTAGT | 1 cut(s) | 2449 |
| BfmI | CTRYAG | 4 cut(s) | 840, 1641, 1898, 3040 |
| BfoI | RGCGCY | 2 cut(s) | 532, 2633 |
| BfuAI | ACCTGC | 1 cut(s) | 1835 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2185 |
| BglII | AGATCT | 1 cut(s) | 143 |
| BlpI | GCTNAGC | 1 cut(s) | 1698 |
| BmcAI | AGTACT | 2 cut(s) | 1446, 2321 |
| Bme1390I | CCNGG | 5 cut(s) | 577, 1437, 2688, 2948, 3305 |
| Bme18I | GGWCC | 3 cut(s) | 134, 718, 3098 |
| BmeT110I | CYCGRG | 3 cut(s) | 524, 2413, 3151 |
| BmgBI | CACGTC | 1 cut(s) | 3259 |
| BmgT120I | GGNCC | 7 cut(s) | 134, 579, 718, 2485, 3098, 3130, 3307 |
| BmiI | GGNNCC | 5 cut(s) | 82, 322, 720, 1047, 2925 |
| BmrFI | CCNGG | 5 cut(s) | 577, 1437, 2688, 2948, 3305 |
| BmrI | ACTGGG | 1 cut(s) | 3304 |
| BmsI | GCATC | 7 cut(s) | 555, 678, 768, 1106, 1126, 2012, 2811 |
| BmuI | ACTGGG | 1 cut(s) | 3304 |
| BpiI | GAAGAC | 2 cut(s) | 343, 1921 |
| BpmI | CTGGAG | 5 cut(s) | 82, 770, 1122, 1209, 2654 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1858, 2808 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1698 |
| BpuEI | CTTGAG | 1 cut(s) | 134 |
| Bsa29I | ATCGAT | 2 cut(s) | 1525, 3242 |
| BsaBI | GATNNNNATC | 1 cut(s) | 494 |
| BsaHI | GRCGYC | 3 cut(s) | 300, 333, 462 |
| BsaI | GGTCTC | 5 cut(s) | 281, 289, 437, 475, 2374 |
| BsaWI | WCCGGW | 3 cut(s) | 250, 317, 2097 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 80, 110, 1182, 1212, 1469, 1499, 2627, 2657 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 752, 1238, 1928, 2032, 2831 |
| Bse118I | RCCGGY | 1 cut(s) | 2097 |
| Bse1I | ACTGG | 4 cut(s) | 753, 984, 2050, 3310 |
| Bse21I | CCTNAGG | 1 cut(s) | 271 |
| Bse3DI | GCAATG | 3 cut(s) | 801, 1089, 1775 |
| Bse8I | GATNNNNATC | 1 cut(s) | 494 |
| BseBI | CCWGG | 5 cut(s) | 577, 1437, 2688, 2948, 3305 |
| BseCI | ATCGAT | 2 cut(s) | 1525, 3242 |
| BseGI | GGATG | 8 cut(s) | 546, 735, 759, 1371, 1721, 2505, 2826, 2871 |
| BseJI | GATNNNNATC | 1 cut(s) | 494 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 752, 1238, 1928, 2032, 2831 |
| BseMI | GCAATG | 3 cut(s) | 801, 1089, 1775 |
| BseMII | CTCAG | 2 cut(s) | 2799, 3354 |
| BseNI | ACTGG | 4 cut(s) | 753, 984, 2050, 3310 |
| BsePI | GCGCGC | 1 cut(s) | 909 |
| BseSI | GKGCMC | 1 cut(s) | 1050 |
| Bsh1236I | CGCG | 4 cut(s) | 25, 521, 911, 948 |
| BshFI | GGCC | 8 cut(s) | 581, 1494, 2179, 2487, 2686, 3017, 3131, 3308 |
| BshNI | GGYRCC | 3 cut(s) | 320, 1045, 2923 |
| BshTI | ACCGGT | 1 cut(s) | 2097 |
| BshVI | ATCGAT | 2 cut(s) | 1525, 3242 |
| BsiHKAI | GWGCWC | 1 cut(s) | 62 |
| BsiHKCI | CYCGRG | 3 cut(s) | 524, 2413, 3151 |
| BsiSI | CCGG | 4 cut(s) | 11, 251, 318, 2098 |
| BslFI | GGGAC | 4 cut(s) | 197, 704, 1363, 2990 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 752, 1238, 1928, 2032, 2831 |
| BsmAI | GTCTC | 7 cut(s) | 281, 289, 437, 475, 2374, 2523, 2782 |
| BsmFI | GGGAC | 4 cut(s) | 197, 704, 1363, 2990 |
| BsmI | GAATGC | 2 cut(s) | 951, 3076 |
| BsnI | GGCC | 8 cut(s) | 581, 1494, 2179, 2487, 2686, 3017, 3131, 3308 |
| Bso31I | GGTCTC | 5 cut(s) | 281, 289, 437, 475, 2374 |
| BsoBI | CYCGRG | 3 cut(s) | 524, 2413, 3151 |
| Bsp1286I | GDGCHC | 3 cut(s) | 62, 1050, 3285 |
| Bsp143I | GATC | 8 cut(s) | 143, 255, 360, 850, 1351, 1645, 3108, 3243 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1698 |
| Bsp19I | CCATGG | 2 cut(s) | 1149, 2364 |
| BspANI | GGCC | 8 cut(s) | 581, 1494, 2179, 2487, 2686, 3017, 3131, 3308 |
| BspCNI | CTCAG | 2 cut(s) | 2800, 3353 |
| BspDI | ATCGAT | 2 cut(s) | 1525, 3242 |
| BspFNI | CGCG | 4 cut(s) | 25, 521, 911, 948 |
| BspLI | GGNNCC | 5 cut(s) | 82, 322, 720, 1047, 2925 |
| BspMAI | CTGCAG | 1 cut(s) | 844 |
| BspMI | ACCTGC | 1 cut(s) | 1835 |
| BspPI | GGATC | 2 cut(s) | 1359, 3116 |
| BspT107I | GGYRCC | 3 cut(s) | 320, 1045, 2923 |
| BspTNI | GGTCTC | 5 cut(s) | 281, 289, 437, 475, 2374 |
| BsrBI | CCGCTC | 1 cut(s) | 1106 |
| BsrDI | GCAATG | 3 cut(s) | 801, 1089, 1775 |
| BsrFI | RCCGGY | 1 cut(s) | 2097 |
| BsrI | ACTGG | 4 cut(s) | 753, 984, 2050, 3310 |
| BssAI | RCCGGY | 1 cut(s) | 2097 |
| BssHII | GCGCGC | 1 cut(s) | 909 |
| BssMI | GATC | 8 cut(s) | 143, 255, 360, 850, 1351, 1645, 3108, 3243 |
| BssNI | GRCGYC | 3 cut(s) | 300, 333, 462 |
| BssT1I | CCWWGG | 4 cut(s) | 69, 1149, 2364, 2432 |
| Bst2UI | CCWGG | 5 cut(s) | 577, 1437, 2688, 2948, 3305 |
| Bst4CI | ACNGT | 4 cut(s) | 1902, 2702, 2848, 3333 |
| BstACI | GRCGYC | 3 cut(s) | 300, 333, 462 |
| BstC8I | GCNNGC | 5 cut(s) | 58, 544, 911, 1704, 2826 |
| BstDEI | CTNAG | 5 cut(s) | 271, 1698, 1858, 2808, 3340 |
| BstDSI | CCRYGG | 5 cut(s) | 23, 706, 904, 1149, 2364 |
| BstEII | GGTNACC | 1 cut(s) | 1072 |
| BstF5I | GGATG | 8 cut(s) | 546, 735, 759, 1371, 1721, 2505, 2826, 2871 |
| BstFNI | CGCG | 4 cut(s) | 25, 521, 911, 948 |
| BstH2I | RGCGCY | 2 cut(s) | 532, 2633 |
| BstHHI | GCGC | 8 cut(s) | 221, 440, 523, 531, 911, 913, 948, 2632 |
| BstKTI | GATC | 8 cut(s) | 146, 258, 363, 853, 1354, 1648, 3111, 3246 |
| BstMAI | GTCTC | 7 cut(s) | 281, 289, 437, 475, 2374, 2523, 2782 |
| BstMBI | GATC | 8 cut(s) | 143, 255, 360, 850, 1351, 1645, 3108, 3243 |
| BstNI | CCWGG | 5 cut(s) | 577, 1437, 2688, 2948, 3305 |
| BstNSI | RCATGY | 2 cut(s) | 1686, 1844 |
| BstPI | GGTNACC | 1 cut(s) | 1072 |
| BstSCI | CCNGG | 5 cut(s) | 575, 1435, 2686, 2946, 3303 |
| BstSFI | CTRYAG | 4 cut(s) | 840, 1641, 1898, 3040 |
| BstSLI | GKGCMC | 1 cut(s) | 1050 |
| BstUI | CGCG | 4 cut(s) | 25, 521, 911, 948 |
| BstV2I | GAAGAC | 2 cut(s) | 343, 1921 |
| BstX2I | RGATCY | 3 cut(s) | 143, 1351, 3108 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 1028, 2088, 2284 |
| BstYI | RGATCY | 3 cut(s) | 143, 1351, 3108 |
| Bsu15I | ATCGAT | 2 cut(s) | 1525, 3242 |
| Bsu36I | CCTNAGG | 1 cut(s) | 271 |
| BsuRI | GGCC | 8 cut(s) | 581, 1494, 2179, 2487, 2686, 3017, 3131, 3308 |
| BsuTUI | ATCGAT | 2 cut(s) | 1525, 3242 |
| BtgI | CCRYGG | 5 cut(s) | 23, 706, 904, 1149, 2364 |
| BtrI | CACGTC | 1 cut(s) | 3259 |
| BtsCI | GGATG | 8 cut(s) | 546, 735, 759, 1371, 1721, 2505, 2826, 2871 |
| BtsI | GCAGTG | 2 cut(s) | 1077, 1158 |
| BtsIMutI | CAGTG | 5 cut(s) | 746, 977, 1077, 1158, 1907 |
| BveI | ACCTGC | 1 cut(s) | 1835 |
| Cac8I | GCNNGC | 5 cut(s) | 58, 544, 911, 1704, 2826 |
| CfoI | GCGC | 8 cut(s) | 221, 440, 523, 531, 911, 913, 948, 2632 |
| Cfr10I | RCCGGY | 1 cut(s) | 2097 |
| Cfr13I | GGNCC | 7 cut(s) | 134, 579, 718, 2485, 3098, 3130, 3307 |
| Cfr42I | CCGCGG | 1 cut(s) | 26 |
| ClaI | ATCGAT | 2 cut(s) | 1525, 3242 |
| CseI | GACGC | 4 cut(s) | 308, 322, 451, 2643 |
| CspAI | ACCGGT | 1 cut(s) | 2097 |
| CspCI | CAANNNNNGTGG | 6 cut(s) | 109, 144, 467, 502, 1593, 1628 |
| DdeI | CTNAG | 5 cut(s) | 271, 1698, 1858, 2808, 3340 |
| DpnI | GATC | 8 cut(s) | 145, 257, 362, 852, 1353, 1647, 3110, 3245 |
| DpnII | GATC | 8 cut(s) | 143, 255, 360, 850, 1351, 1645, 3108, 3243 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2098 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2098 |
| EaeI | YGGCCR | 2 cut(s) | 1492, 2177 |
| EciI | GGCGGA | 1 cut(s) | 580 |
| Eco130I | CCWWGG | 4 cut(s) | 69, 1149, 2364, 2432 |
| Eco147I | AGGCCT | 1 cut(s) | 2686 |
| Eco24I | GRGCYC | 1 cut(s) | 3285 |
| Eco31I | GGTCTC | 5 cut(s) | 281, 289, 437, 475, 2374 |
| Eco32I | GATATC | 2 cut(s) | 1131, 3012 |
| Eco47I | GGWCC | 3 cut(s) | 134, 718, 3098 |
| Eco47III | AGCGCT | 1 cut(s) | 2631 |
| Eco57I | CTGAAG | 5 cut(s) | 358, 1218, 1403, 2312, 3284 |
| Eco81I | CCTNAGG | 1 cut(s) | 271 |
| Eco88I | CYCGRG | 3 cut(s) | 524, 2413, 3151 |
| Eco91I | GGTNACC | 1 cut(s) | 1072 |
| EcoO109I | RGGNCCY | 1 cut(s) | 718 |
| EcoO65I | GGTNACC | 1 cut(s) | 1072 |
| EcoRII | CCWGG | 5 cut(s) | 575, 1435, 2686, 2946, 3303 |
| EcoRV | GATATC | 2 cut(s) | 1131, 3012 |
| EcoT14I | CCWWGG | 4 cut(s) | 69, 1149, 2364, 2432 |
| EcoT22I | ATGCAT | 1 cut(s) | 1501 |
| EcoT38I | GRGCYC | 1 cut(s) | 3285 |
| ErhI | CCWWGG | 4 cut(s) | 69, 1149, 2364, 2432 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1451, 1483 |
| FaqI | GGGAC | 4 cut(s) | 197, 704, 1363, 2990 |
| FauI | CCCGC | 4 cut(s) | 674, 1059, 1099, 3067 |
| FauNDI | CATATG | 2 cut(s) | 2864, 3047 |
| FblI | GTMKAC | 2 cut(s) | 297, 2304 |
| FokI | GGATG | 8 cut(s) | 533, 722, 746, 1378, 1708, 2492, 2833, 2858 |
| FriOI | GRGCYC | 1 cut(s) | 3285 |
| GlaI | GCGC | 8 cut(s) | 220, 439, 522, 530, 910, 912, 947, 2631 |
| GsuI | CTGGAG | 5 cut(s) | 82, 770, 1122, 1209, 2654 |
| HaeII | RGCGCY | 2 cut(s) | 532, 2633 |
| HaeIII | GGCC | 8 cut(s) | 581, 1494, 2179, 2487, 2686, 3017, 3131, 3308 |
| HapII | CCGG | 4 cut(s) | 11, 251, 318, 2098 |
| HgaI | GACGC | 4 cut(s) | 308, 322, 451, 2643 |
| HhaI | GCGC | 8 cut(s) | 221, 440, 523, 531, 911, 913, 948, 2632 |
| Hin1I | GRCGYC | 3 cut(s) | 300, 333, 462 |
| Hin6I | GCGC | 8 cut(s) | 219, 438, 521, 529, 909, 911, 946, 2630 |
| HinP1I | GCGC | 8 cut(s) | 219, 438, 521, 529, 909, 911, 946, 2630 |
| HincII | GTYRAC | 1 cut(s) | 298 |
| HindII | GTYRAC | 1 cut(s) | 298 |
| HindIII | AAGCTT | 3 cut(s) | 1312, 1460, 1821 |
| HpaII | CCGG | 4 cut(s) | 11, 251, 318, 2098 |
| Hpy166II | GTNNAC | 3 cut(s) | 298, 1976, 2305 |
| Hpy8I | GTNNAC | 3 cut(s) | 298, 1976, 2305 |
| Hpy99I | CGWCG | 5 cut(s) | 299, 302, 728, 824, 1653 |
| HpyCH4III | ACNGT | 4 cut(s) | 1902, 2702, 2848, 3333 |
| HpyCH4IV | ACGT | 3 cut(s) | 819, 2093, 3258 |
| HpyF3I | CTNAG | 5 cut(s) | 271, 1698, 1858, 2808, 3340 |
| HpySE526I | ACGT | 3 cut(s) | 819, 2093, 3258 |
| Hsp92I | GRCGYC | 3 cut(s) | 300, 333, 462 |
| HspAI | GCGC | 8 cut(s) | 219, 438, 521, 529, 909, 911, 946, 2630 |
| KspI | CCGCGG | 1 cut(s) | 26 |
| Kzo9I | GATC | 8 cut(s) | 143, 255, 360, 850, 1351, 1645, 3108, 3243 |
| LmnI | GCTCC | 9 cut(s) | 13, 86, 101, 383, 968, 1103, 1151, 1190, 2813 |
| LweI | GCATC | 7 cut(s) | 555, 678, 768, 1106, 1126, 2012, 2811 |
| MaeII | ACGT | 3 cut(s) | 819, 2093, 3258 |
| MaeIII | GTNAC | 6 cut(s) | 922, 1072, 1765, 2224, 2359, 2467 |
| MalI | GATC | 8 cut(s) | 145, 257, 362, 852, 1353, 1647, 3110, 3245 |
| MbiI | CCGCTC | 1 cut(s) | 1106 |
| MboI | GATC | 8 cut(s) | 143, 255, 360, 850, 1351, 1645, 3108, 3243 |
| MfeI | CAATTG | 1 cut(s) | 1980 |
| MflI | RGATCY | 3 cut(s) | 143, 1351, 3108 |
| MhlI | GDGCHC | 3 cut(s) | 62, 1050, 3285 |
| MlsI | TGGCCA | 1 cut(s) | 1494 |
| MluNI | TGGCCA | 1 cut(s) | 1494 |
| MlyI | GAGTC | 6 cut(s) | 104, 689, 1020, 2206, 2945, 3197 |
| Mox20I | TGGCCA | 1 cut(s) | 1494 |
| Mph1103I | ATGCAT | 1 cut(s) | 1501 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1388 |
| MscI | TGGCCA | 1 cut(s) | 1494 |
| MseI | TTAA | 9 cut(s) | 1487, 1533, 2112, 2244, 2499, 2660, 2943, 3231, 3347 |
| MslI | CAYNNNNRTG | 9 cut(s) | 711, 956, 1043, 1082, 1406, 1845, 2086, 2282, 2618 |
| Msp20I | TGGCCA | 1 cut(s) | 1494 |
| MspA1I | CMGCKG | 5 cut(s) | 25, 839, 943, 1228, 2222 |
| MspI | CCGG | 4 cut(s) | 11, 251, 318, 2098 |
| MspR9I | CCNGG | 5 cut(s) | 577, 1437, 2688, 2948, 3305 |
| MunI | CAATTG | 1 cut(s) | 1980 |
| Mva1269I | GAATGC | 2 cut(s) | 951, 3076 |
| MvaI | CCWGG | 5 cut(s) | 577, 1437, 2688, 2948, 3305 |
| MvnI | CGCG | 4 cut(s) | 25, 521, 911, 948 |
| NcoI | CCATGG | 2 cut(s) | 1149, 2364 |
| NdeI | CATATG | 2 cut(s) | 2864, 3047 |
| NdeII | GATC | 8 cut(s) | 143, 255, 360, 850, 1351, 1645, 3108, 3243 |
| NlaIV | GGNNCC | 5 cut(s) | 82, 322, 720, 1047, 2925 |
| NmeAIII | GCCGAG | 2 cut(s) | 219, 466 |
| NmuCI | GTSAC | 3 cut(s) | 922, 1072, 2467 |
| NsiI | ATGCAT | 1 cut(s) | 1501 |
| NspI | RCATGY | 2 cut(s) | 1686, 1844 |
| OliI | CACNNNNGTG | 1 cut(s) | 711 |
| PaeR7I | CTCGAG | 2 cut(s) | 524, 2413 |
| PaqCI | CACCTGC | 1 cut(s) | 1835 |
| PauI | GCGCGC | 1 cut(s) | 909 |
| PceI | AGGCCT | 1 cut(s) | 2686 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 932 |
| PctI | GAATGC | 2 cut(s) | 951, 3076 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1388 |
| PfeI | GAWTC | 7 cut(s) | 162, 195, 490, 931, 1522, 2691, 2748 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1928, 2831 |
| PinAI | ACCGGT | 1 cut(s) | 2097 |
| PleI | GAGTC | 6 cut(s) | 104, 689, 1019, 2206, 2945, 3197 |
| PpsI | GAGTC | 6 cut(s) | 104, 689, 1019, 2206, 2945, 3197 |
| PpuMI | RGGWCCY | 1 cut(s) | 718 |
| PshBI | ATTAAT | 1 cut(s) | 2660 |
| Psp5II | RGGWCCY | 1 cut(s) | 718 |
| Psp6I | CCWGG | 5 cut(s) | 575, 1435, 2686, 2946, 3303 |
| PspEI | GGTNACC | 1 cut(s) | 1072 |
| PspGI | CCWGG | 5 cut(s) | 575, 1435, 2686, 2946, 3303 |
| PspN4I | GGNNCC | 5 cut(s) | 82, 322, 720, 1047, 2925 |
| PspPI | GGNCC | 7 cut(s) | 134, 579, 718, 2485, 3098, 3130, 3307 |
| PspPPI | RGGWCCY | 1 cut(s) | 718 |
| PspXI | VCTCGAGB | 2 cut(s) | 524, 2413 |
| PstI | CTGCAG | 1 cut(s) | 844 |
| PsuI | RGATCY | 3 cut(s) | 143, 1351, 3108 |
| PteI | GCGCGC | 1 cut(s) | 909 |
| PvuII | CAGCTG | 2 cut(s) | 943, 2222 |
| RseI | CAYNNNNRTG | 9 cut(s) | 711, 956, 1043, 1082, 1406, 1845, 2086, 2282, 2618 |
| SacII | CCGCGG | 1 cut(s) | 26 |
| SalI | GTCGAC | 1 cut(s) | 296 |
| SaqAI | TTAA | 9 cut(s) | 1487, 1533, 2112, 2244, 2499, 2660, 2943, 3231, 3347 |
| Sau3AI | GATC | 8 cut(s) | 143, 255, 360, 850, 1351, 1645, 3108, 3243 |
| Sau96I | GGNCC | 7 cut(s) | 134, 579, 718, 2485, 3098, 3130, 3307 |
| ScaI | AGTACT | 2 cut(s) | 1446, 2321 |
| SchI | GAGTC | 6 cut(s) | 104, 689, 1020, 2206, 2945, 3197 |
| ScrFI | CCNGG | 5 cut(s) | 577, 1437, 2688, 2948, 3305 |
| SduI | GDGCHC | 3 cut(s) | 62, 1050, 3285 |
| SfaNI | GCATC | 7 cut(s) | 555, 678, 768, 1106, 1126, 2012, 2811 |
| SfcI | CTRYAG | 4 cut(s) | 840, 1641, 1898, 3040 |
| Sfr274I | CTCGAG | 2 cut(s) | 524, 2413 |
| Sfr303I | CCGCGG | 1 cut(s) | 26 |
| SgrBI | CCGCGG | 1 cut(s) | 26 |
| SgrDI | CGTCGACG | 1 cut(s) | 296 |
| SinI | GGWCC | 3 cut(s) | 134, 718, 3098 |
| SlaI | CTCGAG | 2 cut(s) | 524, 2413 |
| SmiMI | CAYNNNNRTG | 9 cut(s) | 711, 956, 1043, 1082, 1406, 1845, 2086, 2282, 2618 |
| SmlI | CTYRAG | 3 cut(s) | 149, 524, 2413 |
| SmoI | CTYRAG | 3 cut(s) | 149, 524, 2413 |
| SpeI | ACTAGT | 1 cut(s) | 2449 |
| SseBI | AGGCCT | 1 cut(s) | 2686 |
| SspI | AATATT | 1 cut(s) | 1360 |
| StuI | AGGCCT | 1 cut(s) | 2686 |
| StyD4I | CCNGG | 5 cut(s) | 575, 1435, 2686, 2946, 3303 |
| StyI | CCWWGG | 4 cut(s) | 69, 1149, 2364, 2432 |
| TaaI | ACNGT | 4 cut(s) | 1902, 2702, 2848, 3333 |
| TaiI | ACGT | 3 cut(s) | 822, 2096, 3261 |
| TaqI | TCGA | 9 cut(s) | 234, 297, 498, 525, 899, 1258, 1525, 2414, 3242 |
| TaqII | GACCGA | 1 cut(s) | 2588 |
| TatI | WGTACW | 5 cut(s) | 1444, 2319, 2417, 3333, 3351 |
| TauI | GCSGC | 8 cut(s) | 10, 25, 419, 428, 560, 839, 951, 2898 |
| TfiI | GAWTC | 7 cut(s) | 162, 195, 490, 931, 1522, 2691, 2748 |
| Tru1I | TTAA | 9 cut(s) | 1487, 1533, 2112, 2244, 2499, 2660, 2943, 3231, 3347 |
| Tru9I | TTAA | 9 cut(s) | 1487, 1533, 2112, 2244, 2499, 2660, 2943, 3231, 3347 |
| TscAI | CASTG | 5 cut(s) | 753, 984, 1084, 1165, 1907 |
| TseFI | GTSAC | 3 cut(s) | 922, 1072, 2467 |
| Tsp45I | GTSAC | 3 cut(s) | 922, 1072, 2467 |
| TspDTI | ATGAA | 5 cut(s) | 859, 977, 1710, 1832, 2740 |
| TspGWI | ACGGA | 2 cut(s) | 23, 27 |
| TspRI | CASTG | 5 cut(s) | 753, 984, 1084, 1165, 1907 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1928, 2831 |
| VpaK11BI | GGWCC | 3 cut(s) | 134, 718, 3098 |
| VspI | ATTAAT | 1 cut(s) | 2660 |
| XapI | RAATTY | 1 cut(s) | 3124 |
| XbaI | TCTAGA | 2 cut(s) | 140, 3323 |
| XceI | RCATGY | 2 cut(s) | 1686, 1844 |
| XhoI | CTCGAG | 2 cut(s) | 524, 2413 |
| XmiI | GTMKAC | 2 cut(s) | 297, 2304 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1388 |
| ZrmI | AGTACT | 2 cut(s) | 1446, 2321 |
| Zsp2I | ATGCAT | 1 cut(s) | 1501 |