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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G13390 AT5G13390 |
| fragaria_vesca | FvH4_4g22440 |
| malus_domestica | MD13G1132200.v1.1 MD16G1142500.v1.1 |
| prunus_persica | Prupe.1G213300_v2.0.a1 |
| pyrus_communis | pycom13g11500 pycom16g11290 |
| rosa_chinensis | RchiOBHm_Chr4g0402251 RchiOBHm_Chr4g0428701 |
| rosa_laevigata | RLG00000007134 |
| rosa_multiflora | Rmu_sc0003316.1_g000007 |
| rosa_roxburghii | Rroxscaffold_5G00370370 |
| rosa_rugosa | Rorug04G0227300 |
| rosa_samantha | Rh4AG284500 Rh4BG290500 Rh4CG306700 Rh4DG288100 Rh5DG506600 |
| rosa_wichuraiana | Rw0G020630 Rw4G015480 Rw4G024710 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1856 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 2119 |
| AatII | GACGTC | 1 cut(s) | 2163 |
| Acc36I | ACCTGC | 1 cut(s) | 1856 |
| AccB1I | GGYRCC | 6 cut(s) | 341, 989, 1066, 1130, 2944, 3304 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 913, 2852 |
| AccII | CGCG | 5 cut(s) | 25, 181, 203, 227, 863 |
| AccIII | TCCGGA | 1 cut(s) | 3121 |
| AclWI | GGATC | 3 cut(s) | 1380, 1674, 3203 |
| AcoI | YGGCCR | 2 cut(s) | 219, 2198 |
| AcsI | RAATTY | 3 cut(s) | 751, 972, 3145 |
| AcuI | CTGAAG | 6 cut(s) | 82, 124, 379, 637, 1147, 1239 |
| AcyI | GRCGYC | 4 cut(s) | 321, 354, 990, 2160 |
| AfaI | GTAC | 7 cut(s) | 41, 311, 725, 1058, 2395, 2440, 3062 |
| AfeI | AGCGCT | 1 cut(s) | 61 |
| AflIII | ACRYGT | 1 cut(s) | 1703 |
| AgeI | ACCGGT | 1 cut(s) | 2118 |
| AhlI | ACTAGT | 1 cut(s) | 2470 |
| AjnI | CCWGG | 8 cut(s) | 912, 1170, 1456, 1844, 1959, 2349, 2940, 2967 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1073, 1105, 1424, 1456 |
| AleI | CACNNNNGTG | 1 cut(s) | 3056 |
| Alw21I | GWGCWC | 2 cut(s) | 540, 1942 |
| Alw26I | GTCTC | 5 cut(s) | 458, 496, 2167, 2544, 3227 |
| AlwI | GGATC | 3 cut(s) | 1380, 1674, 3203 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 913, 1262 |
| Ama87I | CYCGRG | 2 cut(s) | 545, 3172 |
| Aor13HI | TCCGGA | 1 cut(s) | 3121 |
| Aor51HI | AGCGCT | 1 cut(s) | 61 |
| ApoI | RAATTY | 3 cut(s) | 751, 972, 3145 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 2482, 2514, 2963, 2995 |
| AsiGI | ACCGGT | 1 cut(s) | 2118 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1409 |
| AspA2I | CCTAGG | 2 cut(s) | 292, 2554 |
| AspS9I | GGNCC | 4 cut(s) | 411, 600, 2506, 3119 |
| AsuC2I | CCSGG | 2 cut(s) | 925, 2786 |
| AsuHPI | GGTGA | 2 cut(s) | 1105, 1660 |
| AvaI | CYCGRG | 2 cut(s) | 545, 3172 |
| AvaII | GGWCC | 2 cut(s) | 411, 3119 |
| AvrII | CCTAGG | 2 cut(s) | 292, 2554 |
| BaeGI | GKGCMC | 1 cut(s) | 3307 |
| BalI | TGGCCA | 1 cut(s) | 2200 |
| BanI | GGYRCC | 6 cut(s) | 341, 989, 1066, 1130, 2944, 3304 |
| BanII | GRGCYC | 2 cut(s) | 934, 1942 |
| BauI | CACGAG | 1 cut(s) | 1619 |
| BbsI | GAAGAC | 3 cut(s) | 364, 1660, 3242 |
| Bbv12I | GWGCWC | 2 cut(s) | 540, 1942 |
| BbvCI | CCTCAGC | 1 cut(s) | 2829 |
| BceAI | ACGGC | 6 cut(s) | 66, 163, 206, 599, 745, 977 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 256, 290, 946, 980, 2938, 2972 |
| BciT130I | CCWGG | 8 cut(s) | 914, 1172, 1458, 1846, 1961, 2351, 2942, 2969 |
| BciVI | GTATCC | 1 cut(s) | 1611 |
| BcnI | CCSGG | 2 cut(s) | 925, 2786 |
| BcoDI | GTCTC | 5 cut(s) | 458, 496, 2167, 2544, 3227 |
| BcuI | ACTAGT | 1 cut(s) | 2470 |
| BfmI | CTRYAG | 1 cut(s) | 17 |
| BfoI | RGCGCY | 5 cut(s) | 63, 153, 553, 993, 2785 |
| BfuAI | ACCTGC | 1 cut(s) | 1856 |
| BfuI | GTATCC | 1 cut(s) | 1611 |
| BglI | GCCNNNNNGGC | 3 cut(s) | 218, 2197, 2206 |
| BglII | AGATCT | 1 cut(s) | 164 |
| BlnI | CCTAGG | 2 cut(s) | 292, 2554 |
| Bme18I | GGWCC | 2 cut(s) | 411, 3119 |
| BmeT110I | CYCGRG | 2 cut(s) | 545, 3172 |
| BmgT120I | GGNCC | 4 cut(s) | 411, 600, 2506, 3119 |
| BmsI | GCATC | 7 cut(s) | 789, 1147, 1827, 2033, 2832, 2898, 3288 |
| BpiI | GAAGAC | 3 cut(s) | 364, 1660, 3242 |
| BpmI | CTGGAG | 5 cut(s) | 482, 1143, 1230, 2333, 2675 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2829 |
| BpuEI | CTTGAG | 3 cut(s) | 155, 791, 2419 |
| BpuMI | CCSGG | 2 cut(s) | 925, 2786 |
| BsaBI | GATNNNNATC | 1 cut(s) | 515 |
| BsaHI | GRCGYC | 4 cut(s) | 321, 354, 990, 2160 |
| BsaI | GGTCTC | 3 cut(s) | 458, 496, 2544 |
| BsaWI | WCCGGW | 4 cut(s) | 271, 338, 2118, 3121 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1203, 1233, 2648, 2678 |
| Bse118I | RCCGGY | 2 cut(s) | 437, 2118 |
| Bse1I | ACTGG | 3 cut(s) | 499, 2071, 2102 |
| Bse3DI | GCAATG | 4 cut(s) | 109, 634, 822, 1796 |
| Bse8I | GATNNNNATC | 1 cut(s) | 515 |
| BseAI | TCCGGA | 1 cut(s) | 3121 |
| BseBI | CCWGG | 8 cut(s) | 914, 1172, 1458, 1846, 1961, 2351, 2942, 2969 |
| BseJI | GATNNNNATC | 1 cut(s) | 515 |
| BseMI | GCAATG | 4 cut(s) | 109, 634, 822, 1796 |
| BseMII | CTCAG | 4 cut(s) | 392, 775, 2328, 2820 |
| BseNI | ACTGG | 3 cut(s) | 499, 2071, 2102 |
| BseRI | GAGGAG | 7 cut(s) | 67, 70, 73, 76, 242, 574, 3215 |
| BseSI | GKGCMC | 1 cut(s) | 3307 |
| BseYI | CCCAGC | 3 cut(s) | 1141, 1723, 2987 |
| BsgI | GTGCAG | 1 cut(s) | 1137 |
| Bsh1236I | CGCG | 5 cut(s) | 25, 181, 203, 227, 863 |
| Bsh1285I | CGRYCG | 1 cut(s) | 276 |
| BshNI | GGYRCC | 6 cut(s) | 341, 989, 1066, 1130, 2944, 3304 |
| BshTI | ACCGGT | 1 cut(s) | 2118 |
| BsiEI | CGRYCG | 1 cut(s) | 276 |
| BsiHKAI | GWGCWC | 2 cut(s) | 540, 1942 |
| BsiHKCI | CYCGRG | 2 cut(s) | 545, 3172 |
| BsiSI | CCGG | 8 cut(s) | 11, 272, 339, 438, 924, 2119, 2785, 3122 |
| BslFI | GGGAC | 6 cut(s) | 218, 554, 1384, 1982, 2322, 3011 |
| BsmAI | GTCTC | 5 cut(s) | 458, 496, 2167, 2544, 3227 |
| BsmBI | CGTCTC | 1 cut(s) | 2167 |
| BsmFI | GGGAC | 6 cut(s) | 218, 554, 1384, 1982, 2322, 3011 |
| BsmI | GAATGC | 1 cut(s) | 2526 |
| Bso31I | GGTCTC | 3 cut(s) | 458, 496, 2544 |
| BsoBI | CYCGRG | 2 cut(s) | 545, 3172 |
| Bsp1286I | GDGCHC | 4 cut(s) | 540, 934, 1942, 3307 |
| Bsp13I | TCCGGA | 1 cut(s) | 3121 |
| Bsp1407I | TGTACA | 1 cut(s) | 3060 |
| Bsp143I | GATC | 8 cut(s) | 164, 256, 303, 381, 477, 1372, 1666, 3195 |
| Bsp19I | CCATGG | 3 cut(s) | 2304, 2385, 2453 |
| Bsp68I | TCGCGA | 1 cut(s) | 181 |
| BspCNI | CTCAG | 4 cut(s) | 391, 774, 2329, 2821 |
| BspEI | TCCGGA | 1 cut(s) | 3121 |
| BspFNI | CGCG | 5 cut(s) | 25, 181, 203, 227, 863 |
| BspHI | TCATGA | 2 cut(s) | 300, 981 |
| BspMAI | CTGCAG | 1 cut(s) | 21 |
| BspMI | ACCTGC | 1 cut(s) | 1856 |
| BspPI | GGATC | 3 cut(s) | 1380, 1674, 3203 |
| BspQI | GCTCTTC | 2 cut(s) | 890, 1930 |
| BspT107I | GGYRCC | 6 cut(s) | 341, 989, 1066, 1130, 2944, 3304 |
| BspTNI | GGTCTC | 3 cut(s) | 458, 496, 2544 |
| BsrDI | GCAATG | 4 cut(s) | 109, 634, 822, 1796 |
| BsrFI | RCCGGY | 2 cut(s) | 437, 2118 |
| BsrGI | TGTACA | 1 cut(s) | 3060 |
| BsrI | ACTGG | 3 cut(s) | 499, 2071, 2102 |
| BssAI | RCCGGY | 2 cut(s) | 437, 2118 |
| BssMI | GATC | 8 cut(s) | 164, 256, 303, 381, 477, 1372, 1666, 3195 |
| BssNI | GRCGYC | 4 cut(s) | 321, 354, 990, 2160 |
| BssSI | CACGAG | 1 cut(s) | 1619 |
| BssT1I | CCWWGG | 5 cut(s) | 292, 2304, 2385, 2453, 2554 |
| Bst2BI | CACGAG | 1 cut(s) | 1619 |
| Bst2UI | CCWGG | 8 cut(s) | 914, 1172, 1458, 1846, 1961, 2351, 2942, 2969 |
| Bst4CI | ACNGT | 4 cut(s) | 1659, 2723, 2869, 2927 |
| Bst6I | CTCTTC | 9 cut(s) | 563, 596, 821, 890, 1289, 1930, 2033, 2559, 2619 |
| BstACI | GRCGYC | 4 cut(s) | 321, 354, 990, 2160 |
| BstAUI | TGTACA | 1 cut(s) | 3060 |
| BstDEI | CTNAG | 6 cut(s) | 378, 761, 948, 2047, 2337, 2829 |
| BstDSI | CCRYGG | 6 cut(s) | 23, 727, 861, 2304, 2385, 2453 |
| BstFNI | CGCG | 5 cut(s) | 25, 181, 203, 227, 863 |
| BstH2I | RGCGCY | 5 cut(s) | 63, 153, 553, 993, 2785 |
| BstKTI | GATC | 8 cut(s) | 167, 259, 306, 384, 480, 1375, 1669, 3198 |
| BstMAI | GTCTC | 5 cut(s) | 458, 496, 2167, 2544, 3227 |
| BstMBI | GATC | 8 cut(s) | 164, 256, 303, 381, 477, 1372, 1666, 3195 |
| BstMCI | CGRYCG | 1 cut(s) | 276 |
| BstNI | CCWGG | 8 cut(s) | 914, 1172, 1458, 1846, 1961, 2351, 2942, 2969 |
| BstNSI | RCATGY | 4 cut(s) | 1399, 1707, 1865, 2369 |
| BstSFI | CTRYAG | 1 cut(s) | 17 |
| BstSLI | GKGCMC | 1 cut(s) | 3307 |
| BstUI | CGCG | 5 cut(s) | 25, 181, 203, 227, 863 |
| BstV2I | GAAGAC | 3 cut(s) | 364, 1660, 3242 |
| BstX2I | RGATCY | 2 cut(s) | 164, 1372 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 1049, 2109, 2305 |
| BstYI | RGATCY | 2 cut(s) | 164, 1372 |
| BsuI | GTATCC | 1 cut(s) | 1611 |
| BtgI | CCRYGG | 6 cut(s) | 23, 727, 861, 2304, 2385, 2453 |
| BtgZI | GCGATG | 1 cut(s) | 2164 |
| BtsI | GCAGTG | 4 cut(s) | 1179, 1247, 1928, 3051 |
| BtsIMutI | CAGTG | 4 cut(s) | 1179, 1247, 1928, 3051 |
| BtuMI | TCGCGA | 1 cut(s) | 181 |
| BveI | ACCTGC | 1 cut(s) | 1856 |
| CaiI | CAGNNNCTG | 2 cut(s) | 913, 1262 |
| CciI | TCATGA | 2 cut(s) | 300, 981 |
| Cfr10I | RCCGGY | 2 cut(s) | 437, 2118 |
| Cfr13I | GGNCC | 4 cut(s) | 411, 600, 2506, 3119 |
| Cfr42I | CCGCGG | 2 cut(s) | 26, 864 |
| CseI | GACGC | 2 cut(s) | 329, 343 |
| Csp6I | GTAC | 7 cut(s) | 40, 310, 724, 1057, 2394, 2439, 3061 |
| CspAI | ACCGGT | 1 cut(s) | 2118 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1614, 1649 |
| CviQI | GTAC | 7 cut(s) | 40, 310, 724, 1057, 2394, 2439, 3061 |
| DdeI | CTNAG | 6 cut(s) | 378, 761, 948, 2047, 2337, 2829 |
| DinI | GGCGCC | 1 cut(s) | 991 |
| DpnI | GATC | 8 cut(s) | 166, 258, 305, 383, 479, 1374, 1668, 3197 |
| DpnII | GATC | 8 cut(s) | 164, 256, 303, 381, 477, 1372, 1666, 3195 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2119 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2119 |
| EaeI | YGGCCR | 2 cut(s) | 219, 2198 |
| Eam1104I | CTCTTC | 9 cut(s) | 563, 596, 821, 890, 1289, 1930, 2033, 2559, 2619 |
| EarI | CTCTTC | 9 cut(s) | 563, 596, 821, 890, 1289, 1930, 2033, 2559, 2619 |
| EciI | GGCGGA | 2 cut(s) | 601, 1062 |
| Ecl136II | GAGCTC | 1 cut(s) | 1940 |
| Eco130I | CCWWGG | 5 cut(s) | 292, 2304, 2385, 2453, 2554 |
| Eco147I | AGGCCT | 1 cut(s) | 297 |
| Eco24I | GRGCYC | 2 cut(s) | 934, 1942 |
| Eco31I | GGTCTC | 3 cut(s) | 458, 496, 2544 |
| Eco32I | GATATC | 1 cut(s) | 1152 |
| Eco47I | GGWCC | 2 cut(s) | 411, 3119 |
| Eco47III | AGCGCT | 1 cut(s) | 61 |
| Eco53kI | GAGCTC | 1 cut(s) | 1940 |
| Eco57I | CTGAAG | 6 cut(s) | 82, 124, 379, 637, 1147, 1239 |
| Eco88I | CYCGRG | 2 cut(s) | 545, 3172 |
| EcoICRI | GAGCTC | 1 cut(s) | 1940 |
| EcoRI | GAATTC | 1 cut(s) | 972 |
| EcoRII | CCWGG | 8 cut(s) | 912, 1170, 1456, 1844, 1959, 2349, 2940, 2967 |
| EcoRV | GATATC | 1 cut(s) | 1152 |
| EcoT14I | CCWWGG | 5 cut(s) | 292, 2304, 2385, 2453, 2554 |
| EcoT22I | ATGCAT | 1 cut(s) | 2891 |
| EcoT38I | GRGCYC | 2 cut(s) | 934, 1942 |
| EgeI | GGCGCC | 1 cut(s) | 991 |
| EheI | GGCGCC | 1 cut(s) | 991 |
| ErhI | CCWWGG | 5 cut(s) | 292, 2304, 2385, 2453, 2554 |
| Esp3I | CGTCTC | 1 cut(s) | 2167 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1472, 1504, 2030, 2062 |
| FaqI | GGGAC | 6 cut(s) | 218, 554, 1384, 1982, 2322, 3011 |
| FauI | CCCGC | 5 cut(s) | 143, 199, 816, 960, 3294 |
| FauNDI | CATATG | 2 cut(s) | 2885, 3068 |
| FriOI | GRGCYC | 2 cut(s) | 934, 1942 |
| GsaI | CCCAGC | 3 cut(s) | 1145, 1727, 2991 |
| GsuI | CTGGAG | 5 cut(s) | 482, 1143, 1230, 2333, 2675 |
| HaeII | RGCGCY | 5 cut(s) | 63, 153, 553, 993, 2785 |
| HapII | CCGG | 8 cut(s) | 11, 272, 339, 438, 924, 2119, 2785, 3122 |
| HgaI | GACGC | 2 cut(s) | 329, 343 |
| Hin1I | GRCGYC | 4 cut(s) | 321, 354, 990, 2160 |
| HindIII | AAGCTT | 1 cut(s) | 2573 |
| HpaII | CCGG | 8 cut(s) | 11, 272, 339, 438, 924, 2119, 2785, 3122 |
| HphI | GGTGA | 2 cut(s) | 1105, 1660 |
| Hpy166II | GTNNAC | 3 cut(s) | 411, 2394, 3061 |
| Hpy8I | GTNNAC | 3 cut(s) | 411, 2394, 3061 |
| Hpy99I | CGWCG | 4 cut(s) | 227, 281, 323, 406 |
| HpyCH4III | ACNGT | 4 cut(s) | 1659, 2723, 2869, 2927 |
| HpyCH4IV | ACGT | 1 cut(s) | 2160 |
| HpyF3I | CTNAG | 6 cut(s) | 378, 761, 948, 2047, 2337, 2829 |
| HpySE526I | ACGT | 1 cut(s) | 2160 |
| Hsp92I | GRCGYC | 4 cut(s) | 321, 354, 990, 2160 |
| KasI | GGCGCC | 1 cut(s) | 989 |
| Kpn2I | TCCGGA | 1 cut(s) | 3121 |
| KroI | GCCGGC | 1 cut(s) | 437 |
| KroNI | GCCGGC | 1 cut(s) | 439 |
| KspI | CCGCGG | 2 cut(s) | 26, 864 |
| Kzo9I | GATC | 8 cut(s) | 164, 256, 303, 381, 477, 1372, 1666, 3195 |
| LguI | GCTCTTC | 2 cut(s) | 890, 1930 |
| LweI | GCATC | 7 cut(s) | 789, 1147, 1827, 2033, 2832, 2898, 3288 |
| MaeII | ACGT | 1 cut(s) | 2160 |
| MalI | GATC | 8 cut(s) | 166, 258, 305, 383, 479, 1374, 1668, 3197 |
| MboI | GATC | 8 cut(s) | 164, 256, 303, 381, 477, 1372, 1666, 3195 |
| MfeI | CAATTG | 1 cut(s) | 531 |
| MflI | RGATCY | 2 cut(s) | 164, 1372 |
| MhlI | GDGCHC | 4 cut(s) | 540, 934, 1942, 3307 |
| MlsI | TGGCCA | 1 cut(s) | 2200 |
| MluNI | TGGCCA | 1 cut(s) | 2200 |
| Mly113I | GGCGCC | 1 cut(s) | 990 |
| MlyI | GAGTC | 2 cut(s) | 125, 2966 |
| MmeI | TCCRAC | 2 cut(s) | 114, 1204 |
| Mox20I | TGGCCA | 1 cut(s) | 2200 |
| Mph1103I | ATGCAT | 1 cut(s) | 2891 |
| MroI | TCCGGA | 1 cut(s) | 3121 |
| MroNI | GCCGGC | 1 cut(s) | 437 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1409 |
| MscI | TGGCCA | 1 cut(s) | 2200 |
| MseI | TTAA | 4 cut(s) | 1554, 2133, 2520, 2897 |
| MslI | CAYNNNNRTG | 5 cut(s) | 1064, 1866, 2303, 3056, 3090 |
| Msp20I | TGGCCA | 1 cut(s) | 2200 |
| MspA1I | CMGCKG | 7 cut(s) | 25, 657, 863, 964, 1121, 1769, 2357 |
| MspI | CCGG | 8 cut(s) | 11, 272, 339, 438, 924, 2119, 2785, 3122 |
| MunI | CAATTG | 1 cut(s) | 531 |
| Mva1269I | GAATGC | 1 cut(s) | 2526 |
| MvaI | CCWGG | 8 cut(s) | 914, 1172, 1458, 1846, 1961, 2351, 2942, 2969 |
| MvnI | CGCG | 5 cut(s) | 25, 181, 203, 227, 863 |
| NaeI | GCCGGC | 1 cut(s) | 439 |
| NarI | GGCGCC | 1 cut(s) | 990 |
| NciI | CCSGG | 2 cut(s) | 925, 2786 |
| NcoI | CCATGG | 3 cut(s) | 2304, 2385, 2453 |
| NdeI | CATATG | 2 cut(s) | 2885, 3068 |
| NdeII | GATC | 8 cut(s) | 164, 256, 303, 381, 477, 1372, 1666, 3195 |
| NgoMIV | GCCGGC | 1 cut(s) | 437 |
| NmeAIII | GCCGAG | 2 cut(s) | 240, 487 |
| NmuCI | GTSAC | 6 cut(s) | 943, 1093, 1510, 1532, 1621, 2488 |
| NruI | TCGCGA | 1 cut(s) | 181 |
| NsiI | ATGCAT | 1 cut(s) | 2891 |
| NspI | RCATGY | 4 cut(s) | 1399, 1707, 1865, 2369 |
| OliI | CACNNNNGTG | 1 cut(s) | 3056 |
| PaeR7I | CTCGAG | 1 cut(s) | 545 |
| PagI | TCATGA | 2 cut(s) | 300, 981 |
| PaqCI | CACCTGC | 1 cut(s) | 1856 |
| PceI | AGGCCT | 1 cut(s) | 297 |
| PciI | ACATGT | 1 cut(s) | 1703 |
| PciSI | GCTCTTC | 2 cut(s) | 890, 1930 |
| PctI | GAATGC | 1 cut(s) | 2526 |
| PdiI | GCCGGC | 1 cut(s) | 439 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1409 |
| PfeI | GAWTC | 8 cut(s) | 183, 430, 511, 523, 716, 952, 2712, 2769 |
| PflFI | GACNNNGTC | 1 cut(s) | 2721 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 913, 2852 |
| PinAI | ACCGGT | 1 cut(s) | 2118 |
| PleI | GAGTC | 2 cut(s) | 125, 2966 |
| PluTI | GGCGCC | 1 cut(s) | 993 |
| PpsI | GAGTC | 2 cut(s) | 125, 2966 |
| PscI | ACATGT | 1 cut(s) | 1703 |
| Psp124BI | GAGCTC | 1 cut(s) | 1942 |
| Psp6I | CCWGG | 8 cut(s) | 912, 1170, 1456, 1844, 1959, 2349, 2940, 2967 |
| PspFI | CCCAGC | 3 cut(s) | 1141, 1723, 2987 |
| PspGI | CCWGG | 8 cut(s) | 912, 1170, 1456, 1844, 1959, 2349, 2940, 2967 |
| PspPI | GGNCC | 4 cut(s) | 411, 600, 2506, 3119 |
| PspXI | VCTCGAGB | 1 cut(s) | 545 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 785, 817 |
| PstI | CTGCAG | 1 cut(s) | 21 |
| PstNI | CAGNNNCTG | 2 cut(s) | 913, 1262 |
| PsuI | RGATCY | 2 cut(s) | 164, 1372 |
| PsyI | GACNNNGTC | 1 cut(s) | 2721 |
| PvuII | CAGCTG | 5 cut(s) | 657, 964, 1121, 1769, 2357 |
| RruI | TCGCGA | 1 cut(s) | 181 |
| RsaI | GTAC | 7 cut(s) | 41, 311, 725, 1058, 2395, 2440, 3062 |
| RsaNI | GTAC | 7 cut(s) | 40, 310, 724, 1057, 2394, 2439, 3061 |
| RseI | CAYNNNNRTG | 5 cut(s) | 1064, 1866, 2303, 3056, 3090 |
| SacI | GAGCTC | 1 cut(s) | 1942 |
| SacII | CCGCGG | 2 cut(s) | 26, 864 |
| SapI | GCTCTTC | 2 cut(s) | 890, 1930 |
| SaqAI | TTAA | 4 cut(s) | 1554, 2133, 2520, 2897 |
| Sau3AI | GATC | 8 cut(s) | 164, 256, 303, 381, 477, 1372, 1666, 3195 |
| Sau96I | GGNCC | 4 cut(s) | 411, 600, 2506, 3119 |
| SchI | GAGTC | 2 cut(s) | 125, 2966 |
| SduI | GDGCHC | 4 cut(s) | 540, 934, 1942, 3307 |
| SfaNI | GCATC | 7 cut(s) | 789, 1147, 1827, 2033, 2832, 2898, 3288 |
| SfcI | CTRYAG | 1 cut(s) | 17 |
| SfoI | GGCGCC | 1 cut(s) | 991 |
| Sfr274I | CTCGAG | 1 cut(s) | 545 |
| Sfr303I | CCGCGG | 2 cut(s) | 26, 864 |
| SgrBI | CCGCGG | 2 cut(s) | 26, 864 |
| SinI | GGWCC | 2 cut(s) | 411, 3119 |
| SlaI | CTCGAG | 1 cut(s) | 545 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 1064, 1866, 2303, 3056, 3090 |
| SmlI | CTYRAG | 4 cut(s) | 170, 545, 770, 2434 |
| SmoI | CTYRAG | 4 cut(s) | 170, 545, 770, 2434 |
| SpeI | ACTAGT | 1 cut(s) | 2470 |
| SseBI | AGGCCT | 1 cut(s) | 297 |
| SspDI | GGCGCC | 1 cut(s) | 989 |
| SspI | AATATT | 2 cut(s) | 1381, 2934 |
| SstI | GAGCTC | 1 cut(s) | 1942 |
| StuI | AGGCCT | 1 cut(s) | 297 |
| StyI | CCWWGG | 5 cut(s) | 292, 2304, 2385, 2453, 2554 |
| TaaI | ACNGT | 4 cut(s) | 1659, 2723, 2869, 2927 |
| TaiI | ACGT | 1 cut(s) | 2163 |
| TaqI | TCGA | 5 cut(s) | 255, 318, 519, 546, 1672 |
| TatI | WGTACW | 1 cut(s) | 3060 |
| TfiI | GAWTC | 8 cut(s) | 183, 430, 511, 523, 716, 952, 2712, 2769 |
| Tru1I | TTAA | 4 cut(s) | 1554, 2133, 2520, 2897 |
| Tru9I | TTAA | 4 cut(s) | 1554, 2133, 2520, 2897 |
| TscAI | CASTG | 4 cut(s) | 1186, 1254, 1928, 3058 |
| TseFI | GTSAC | 6 cut(s) | 943, 1093, 1510, 1532, 1621, 2488 |
| Tsp45I | GTSAC | 6 cut(s) | 943, 1093, 1510, 1532, 1621, 2488 |
| TspDTI | ATGAA | 8 cut(s) | 880, 998, 1035, 1731, 2167, 2629, 2682, 2761 |
| TspGWI | ACGGA | 2 cut(s) | 23, 27 |
| TspRI | CASTG | 4 cut(s) | 1186, 1254, 1928, 3058 |
| Tth111I | GACNNNGTC | 1 cut(s) | 2721 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 913, 2852 |
| VpaK11BI | GGWCC | 2 cut(s) | 411, 3119 |
| XapI | RAATTY | 3 cut(s) | 751, 972, 3145 |
| XbaI | TCTAGA | 1 cut(s) | 161 |
| XceI | RCATGY | 4 cut(s) | 1399, 1707, 1865, 2369 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2193 |
| XhoI | CTCGAG | 1 cut(s) | 545 |
| XmaJI | CCTAGG | 2 cut(s) | 292, 2554 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1409 |
| ZraI | GACGTC | 1 cut(s) | 2161 |
| Zsp2I | ATGCAT | 1 cut(s) | 2891 |