Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0009466

Physical Location & Seq

Genomic Coordinates Forward (+)

59955369 .. 59959873

Gene Structure 4505 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG284500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3360 bp

ATGATGCCGCCGGAGCTGCAGCCGCGGTTGTTCCGTCCGTACATCTCAACGGCGGCGAGCGCTTCTTCTTCTCTCTTCAGCAATGGTTCCCCGAACCCTAGCCCCACCGACTCCCGCTTCAGCAACGGCGCTGCTGCTTCTAGATCTCTCAAGAACTCGCGATTCACGGCCGCCGCCTTCGCGCACAACGCCAGAATGGCCGTCGCGCTCGTCCCCTGCGCCGCCTTCCTCCTCGATCTCGGCGGCACTCCGGTCGTCGCGACCCTAACCCTAGGCCTGATGATCTCCTACATCATCGACGCCCTCAACTTCAAGTCCGGTGCCTTCTTCGGCGTCTGGTTCTCCCTCGTCTTCTCTCAGATCGCCTTCTTCTTCAGCTCGTCGCTGTTGACCTCCTTCAATTCGTGGATTCTCGCCGGCCTCGCCGCCTTCCTCTGCGCCGAGACCGATTTCTTGATCGGCGTATGGGTCTCCCTCCAGTTCAAGTGGATTCAAATCGAGAATCCTTCAATTGTGCTCGCCCTCGAGCGCCTCCTCTTCGCCTGTGTCCCCTTCGCCGCCTCCTCACTCTTCACTTGGGCCACCATCTCCGCCGTCGGTATGAGCAATGCTTCTTACTACCTCATGGCCTTCAGCTGCGTTTTCTATTGGCTCTACTCCATCCCTCGCATTTCGTCTTTCAAGACCAAGCAGGATTCCAAGTACCACGGCGGCGAGGTTCCCGATGAGAATTTGATACTCAGCCCCCTCGAGAGTTGCATCCACACTCTGTATCTGCTGTTCTTCCCGCTACTCTTCCACATTGCCTCTCACTACTCCATCATGTTCTCATCCGCCGCCGCAGTTTCCGATTTGTTCCTGCTCTTCTTCATTCCTTTCCTGTTCCAGCTCCTGGCCTCTACCAGGGGAGCCCTCTGGTGGGTCACTAAGAATCCGAACCAGCTGCGAGGAATTCAAGTCATGAATGGCGCCGTTGCTTTGGTTGTTGTTGTAATCTGTTTGGAGATTAGAGTCGTTTTCCATTCATTTGGGCGGTACATTCAGGTGCCTCCGCCTTTGAATTACCTTTTGGTGACTACTACAATGCTTGGAGGTGCAGCTGGAGCTGGTGCCTATGCGCTGGGTATGATATCTGATGCCTTCAGCTCCCTGGCTTTCACTGCTCTGGCTGTTGTAGTTAGTGCTGCTGGAGCTATTGTTGTCGGATTTCCTGTTTTGTTCCTTCCACTGCCTTCAGTTGCTGGCTTCTATTTGGCTCGCTTTTTTACAAAGAAGAGTGTTCCATCCTACTTTGCTTTTGTTGTGCTTGGGAGCCTGATGGTTACATGGTTTGTAATGCACAATTTCTGGGATCTAAATATTTGGATGGCAGGCATGTCCCTGAAATCCTTCTGCAAACTTGTTATTTTGAATGTTGTCTTGGCCTTGACTATTCCTGGTCTAGCTCTACTTCCTTCGAAGCTCCACTTTTTGACAGAGATTGGTTTAGTCACTCATGCATTGCTTTTATCTCACATTGAGAACCGATTTTTTAATTACTCTGGGATATATTATTATGGATTTGAGGATGATGTAATGTATCCAAGCTACATGGTTCTCGTGACAACATTTGTGGGTTTAGCTTTGGTGAGAAGACTGTCTGCGGATCATCGAATTGGTGCGAAGGCAGTTTGGATTTTGACATGTCTTTATTCTGCAAAGCTGGGTATGCTAGTGATTTCATCAAAGTCGGTTGTCTGGATGTCAGCTGTTCTATTGTTGGCTGTAACGCCACCATTGCTTCTCTACAAGGATAAGTCAAGGACAGCCTCAAAGATGCAAACCTGGCAAGGTTATACACACGCAGGTGTGGTTTCCTTATCAGTTTGGTTTTGTCGTGAAACAATCTTTGAAGCCTTGCAGTGGTGGAATGGAAGAGCTCCCTCTGATGGCCTACTCCTGGGCTTCTGTATTGTCTTGATGGGCTTGGCTTGTGTCCCTATTGTGGCTCTCCACTTCTCTCATGTCTTGCCTGCGAAGAGATGCTTAGTGCTGGTGGTGGCTACTGGCTTGCTCTTTATCCTTTTGCAACCACCTATTCCAGTATCATGGACTTACCGGTCTGACCTAATTAAAGCTGCCCGTCAGTCTGTTGATGACGTCTCCATATACGGGTTCATCGCACCAAAGCCTATGTGGCCATCATGGCTGCTAATTGTGGCTATCCTGCTTACTCTAGCTGCTGTTACATCTGTTATACCAATCAAATACATGGTTGAGTTGAGAGTGTTTTATTCCATAGCCATGGGACTTGCTCTAGGTATCTACATATCTACTGAGTTCTTCCTCCAGGCAGCTGTCTTGCATGTCCTCATTGTTGTAACCATGGTGTGTACCTCTGTGTTTGTGGTTTTCACTCATTTCCCATCTGCCTCAAGTACGAAGCTGCTTCCATGGATATTTGCTTTACTAGTAGCTCTCTTTCCTGTGACATATCTTTTGGAGGGCCAAGTGAGAATTAAAAGCATTCTTGGGGATGGTGGATTTGGAGACCTAGGAGAAGAAGAGAGGAAGCTTACAACTCTATTTGCTGTTGAGGGGGCAAGGACATCTCTTCTTGGTCTTTATGCAGCAATTTTCATGCTAGTAGCTCTGGAGGTAAAGTATGAACTCGCCTCATTATTGAGGGAAAAGGCTACTGAAAGGAGTGGAATCAGACACAGTCTTTCTGGTCAAAGCACATCTACTAGCTTTCCTTCAAGAATGAGATTCATGCAACAGCGCCGGGCTTCTAGTATCTCATCCTTCACAATCAAGAAAATGACTGCTGAGGGAGCATGGATGCCAGCAGTTGGTAATGTTGCTACTGTGATGTGCTTTGCCATATGCATCATCTTAAATGTCAATATCACAGGTGGCTCAAACCGTGCAATATTCTTCCTGGCACCGATTTTGCTGCTACTCAACCAGGACTCAGATTTTGTTGCTGGGTTTGGGGACAAGCAGAGGTATTTCCCTGTTACAATAGTTATATCATCCTACCTAGTCATCACTGCTGTGTACAGCATATGGGAAGAAATCTGGCATGGGAATGTAGGTTGGGGATTGGAAATCGGTGGTCCGGATTGGTTCTTTGCTGTGAAGAATTTGGCACTCCTTATTCTTACATTCCCGAGCCATATCCTTTTCAACAGGTATGTGTGGAGTTTAACAAAGCAGACAGACTCAACTCCATTGATAACTATGCCCCTTAATTTGCCATCTGTAATAATAACAGATGTGCTGAAGGTTAGGATATTAGGTCTCTTAGGAATTATTTATTCCCTAGCCCAGTATCTAGTATCTAGACAACAGTACATCTCAGGATTGAAGTATATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1119

Amino Acids

123.36

Weight (kDa)

9.19

Isoelectric Point (pI)

40.2

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009466)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13390 AT5G13390
fragaria_vesca	FvH4_4g22440
malus_domestica	MD13G1132200.v1.1 MD16G1142500.v1.1
prunus_persica	Prupe.1G213300_v2.0.a1
pyrus_communis	pycom13g11500 pycom16g11290
rosa_chinensis	RchiOBHm_Chr4g0402251 RchiOBHm_Chr4g0428701
rosa_laevigata	RLG00000007134
rosa_multiflora	Rmu_sc0003316.1_g000007
rosa_roxburghii	Rroxscaffold_5G00370370
rosa_rugosa	Rorug04G0227300
rosa_samantha	Rh4AG284500 Rh4BG290500 Rh4CG306700 Rh4DG288100 Rh5DG506600
rosa_wichuraiana	Rw0G020630 Rw4G015480 Rw4G024710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1835
AasI	GACNNNNNNGTC	1 cut(s)	2098
AatII	GACGTC	1 cut(s)	2142
Acc36I	ACCTGC	1 cut(s)	1835
AccB1I	GGYRCC	5 cut(s)	320, 968, 1045, 1109, 2923
AccB7I	CCANNNNNTGG	2 cut(s)	892, 2831
AccII	CGCG	5 cut(s)	25, 160, 182, 206, 260
AccIII	TCCGGA	1 cut(s)	3100
AclWI	GGATC	2 cut(s)	1359, 1653
AcoI	YGGCCR	3 cut(s)	168, 198, 2177
AcsI	RAATTY	3 cut(s)	730, 951, 3124
AcuI	CTGAAG	7 cut(s)	61, 103, 358, 616, 1126, 1218, 3284
AcyI	GRCGYC	4 cut(s)	300, 333, 969, 2139
AfaI	GTAC	7 cut(s)	41, 704, 1037, 2374, 2419, 3041, 3335
AfeI	AGCGCT	1 cut(s)	61
AflIII	ACRYGT	1 cut(s)	1682
AgeI	ACCGGT	1 cut(s)	2097
AhlI	ACTAGT	1 cut(s)	2449
AjnI	CCWGG	9 cut(s)	891, 902, 1149, 1435, 1823, 1938, 2328, 2919, 2946
AjuI	GAANNNNNNNTTGG	4 cut(s)	1052, 1084, 1403, 1435
AleI	CACNNNNGTG	2 cut(s)	1845, 3035
Alw21I	GWGCWC	2 cut(s)	519, 1921
Alw26I	GTCTC	5 cut(s)	437, 475, 2146, 2523, 3287
AlwI	GGATC	2 cut(s)	1359, 1653
AlwNI	CAGNNNCTG	2 cut(s)	892, 1241
Ama87I	CYCGRG	3 cut(s)	524, 749, 3151
Aor13HI	TCCGGA	1 cut(s)	3100
Aor51HI	AGCGCT	1 cut(s)	61
ApoI	RAATTY	3 cut(s)	730, 951, 3124
ArsI	GACNNNNNNTTYG	4 cut(s)	2461, 2493, 2942, 2974
AsiGI	ACCGGT	1 cut(s)	2097
Asp700I	GAANNNNTTC	1 cut(s)	1388
AspA2I	CCTAGG	2 cut(s)	271, 2533
AspS9I	GGNCC	3 cut(s)	579, 2485, 3098
AsuC2I	CCSGG	1 cut(s)	2765
AsuHPI	GGTGA	2 cut(s)	1084, 1639
AsuII	TTCGAA	1 cut(s)	1457
AvaI	CYCGRG	3 cut(s)	524, 749, 3151
AvaII	GGWCC	1 cut(s)	3098
AvrII	CCTAGG	2 cut(s)	271, 2533
BalI	TGGCCA	1 cut(s)	2179
BanI	GGYRCC	5 cut(s)	320, 968, 1045, 1109, 2923
BanII	GRGCYC	2 cut(s)	913, 1921
BauI	CACGAG	1 cut(s)	1598
BbsI	GAAGAC	2 cut(s)	343, 1639
Bbv12I	GWGCWC	2 cut(s)	519, 1921
BbvCI	CCTCAGC	1 cut(s)	2808
BceAI	ACGGC	7 cut(s)	66, 142, 183, 185, 578, 724, 956
BcgI	CGANNNNNNTGC	6 cut(s)	235, 269, 925, 959, 2917, 2951
BciT130I	CCWGG	9 cut(s)	893, 904, 1151, 1437, 1825, 1940, 2330, 2921, 2948
BciVI	GTATCC	1 cut(s)	1590
BcnI	CCSGG	1 cut(s)	2765
BcoDI	GTCTC	5 cut(s)	437, 475, 2146, 2523, 3287
BcuI	ACTAGT	1 cut(s)	2449
BfmI	CTRYAG	1 cut(s)	17
BfoI	RGCGCY	5 cut(s)	63, 132, 532, 972, 2764
BfuAI	ACCTGC	1 cut(s)	1835
BfuI	GTATCC	1 cut(s)	1590
BglI	GCCNNNNNGGC	3 cut(s)	197, 2176, 2185
BglII	AGATCT	1 cut(s)	143
BlnI	CCTAGG	2 cut(s)	271, 2533
Bme18I	GGWCC	1 cut(s)	3098
BmeT110I	CYCGRG	3 cut(s)	524, 749, 3151
BmgT120I	GGNCC	3 cut(s)	579, 2485, 3098
BmiI	GGNNCC	9 cut(s)	88, 322, 720, 910, 970, 1047, 1111, 1313, 2925
BmrI	ACTGGG	1 cut(s)	3304
BmsI	GCATC	6 cut(s)	768, 1126, 1806, 2012, 2811, 2877
BmuI	ACTGGG	1 cut(s)	3304
BpiI	GAAGAC	2 cut(s)	343, 1639
BpmI	CTGGAG	5 cut(s)	461, 1122, 1209, 2312, 2654
Bpu10I	CCTNAGC	1 cut(s)	2808
Bpu14I	TTCGAA	1 cut(s)	1457
BpuEI	CTTGAG	2 cut(s)	134, 2398
BpuMI	CCSGG	1 cut(s)	2765
BsaBI	GATNNNNATC	1 cut(s)	494
BsaHI	GRCGYC	4 cut(s)	300, 333, 969, 2139
BsaI	GGTCTC	4 cut(s)	437, 475, 2523, 3287
BsaWI	WCCGGW	4 cut(s)	250, 317, 2097, 3100
BsaXI	ACNNNNNCTCC	4 cut(s)	1182, 1212, 2627, 2657
Bse118I	RCCGGY	2 cut(s)	416, 2097
Bse1I	ACTGG	4 cut(s)	478, 2050, 2081, 3310
Bse3DI	GCAATG	5 cut(s)	88, 613, 801, 1499, 1775
Bse8I	GATNNNNATC	1 cut(s)	494
BseAI	TCCGGA	1 cut(s)	3100
BseBI	CCWGG	9 cut(s)	893, 904, 1151, 1437, 1825, 1940, 2330, 2921, 2948
BseJI	GATNNNNATC	1 cut(s)	494
BseMI	GCAATG	5 cut(s)	88, 613, 801, 1499, 1775
BseMII	CTCAG	6 cut(s)	371, 754, 2307, 2799, 2967, 3354
BseNI	ACTGG	4 cut(s)	478, 2050, 2081, 3310
BseRI	GAGGAG	3 cut(s)	221, 524, 553
BseX3I	CGGCCG	1 cut(s)	168
BseYI	CCCAGC	3 cut(s)	1120, 1702, 2966
BsgI	GTGCAG	1 cut(s)	1116
Bsh1236I	CGCG	5 cut(s)	25, 160, 182, 206, 260
Bsh1285I	CGRYCG	2 cut(s)	171, 255
BshNI	GGYRCC	5 cut(s)	320, 968, 1045, 1109, 2923
BshTI	ACCGGT	1 cut(s)	2097
BsiEI	CGRYCG	2 cut(s)	171, 255
BsiHKAI	GWGCWC	2 cut(s)	519, 1921
BsiHKCI	CYCGRG	3 cut(s)	524, 749, 3151
BsiSI	CCGG	7 cut(s)	11, 251, 318, 417, 2098, 2764, 3101
BslFI	GGGAC	6 cut(s)	197, 533, 1363, 1961, 2301, 2990
BsmAI	GTCTC	5 cut(s)	437, 475, 2146, 2523, 3287
BsmBI	CGTCTC	1 cut(s)	2146
BsmFI	GGGAC	6 cut(s)	197, 533, 1363, 1961, 2301, 2990
BsmI	GAATGC	1 cut(s)	2505
Bso31I	GGTCTC	4 cut(s)	437, 475, 2523, 3287
BsoBI	CYCGRG	3 cut(s)	524, 749, 3151
Bsp119I	TTCGAA	1 cut(s)	1457
Bsp1286I	GDGCHC	3 cut(s)	519, 913, 1921
Bsp13I	TCCGGA	1 cut(s)	3100
Bsp1407I	TGTACA	1 cut(s)	3039
Bsp143I	GATC	7 cut(s)	143, 235, 282, 360, 456, 1351, 1645
Bsp19I	CCATGG	3 cut(s)	2283, 2364, 2432
Bsp68I	TCGCGA	2 cut(s)	160, 260
BspCNI	CTCAG	6 cut(s)	370, 753, 2308, 2800, 2966, 3353
BspEI	TCCGGA	1 cut(s)	3100
BspFNI	CGCG	5 cut(s)	25, 160, 182, 206, 260
BspHI	TCATGA	1 cut(s)	960
BspLI	GGNNCC	9 cut(s)	88, 322, 720, 910, 970, 1047, 1111, 1313, 2925
BspMAI	CTGCAG	1 cut(s)	21
BspMI	ACCTGC	1 cut(s)	1835
BspPI	GGATC	2 cut(s)	1359, 1653
BspQI	GCTCTTC	2 cut(s)	869, 1909
BspT104I	TTCGAA	1 cut(s)	1457
BspT107I	GGYRCC	5 cut(s)	320, 968, 1045, 1109, 2923
BspTNI	GGTCTC	4 cut(s)	437, 475, 2523, 3287
BsrDI	GCAATG	5 cut(s)	88, 613, 801, 1499, 1775
BsrFI	RCCGGY	2 cut(s)	416, 2097
BsrGI	TGTACA	1 cut(s)	3039
BsrI	ACTGG	4 cut(s)	478, 2050, 2081, 3310
BssAI	RCCGGY	2 cut(s)	416, 2097
BssMI	GATC	7 cut(s)	143, 235, 282, 360, 456, 1351, 1645
BssNI	GRCGYC	4 cut(s)	300, 333, 969, 2139
BssSI	CACGAG	1 cut(s)	1598
BssT1I	CCWWGG	5 cut(s)	271, 2283, 2364, 2432, 2533
Bst2BI	CACGAG	1 cut(s)	1598
Bst2UI	CCWGG	9 cut(s)	893, 904, 1151, 1437, 1825, 1940, 2330, 2921, 2948
Bst4CI	ACNGT	5 cut(s)	1638, 2702, 2848, 2906, 3333
BstACI	GRCGYC	4 cut(s)	300, 333, 969, 2139
BstAUI	TGTACA	1 cut(s)	3039
BstBI	TTCGAA	1 cut(s)	1457
BstC8I	GCNNGC	9 cut(s)	58, 418, 519, 1243, 1258, 1372, 2013, 2051, 2826
BstDEI	CTNAG	9 cut(s)	357, 740, 927, 2026, 2316, 2808, 2953, 3286, 3340
BstDSI	CCRYGG	5 cut(s)	23, 706, 2283, 2364, 2432
BstENI	CCTNNNNNAGG	1 cut(s)	901
BstFNI	CGCG	5 cut(s)	25, 160, 182, 206, 260
BstH2I	RGCGCY	5 cut(s)	63, 132, 532, 972, 2764
BstKTI	GATC	7 cut(s)	146, 238, 285, 363, 459, 1354, 1648
BstMAI	GTCTC	5 cut(s)	437, 475, 2146, 2523, 3287
BstMBI	GATC	7 cut(s)	143, 235, 282, 360, 456, 1351, 1645
BstMCI	CGRYCG	2 cut(s)	171, 255
BstNI	CCWGG	9 cut(s)	893, 904, 1151, 1437, 1825, 1940, 2330, 2921, 2948
BstNSI	RCATGY	3 cut(s)	1378, 1686, 2348
BstSFI	CTRYAG	1 cut(s)	17
BstUI	CGCG	5 cut(s)	25, 160, 182, 206, 260
BstV2I	GAAGAC	2 cut(s)	343, 1639
BstX2I	RGATCY	2 cut(s)	143, 1351
BstXI	CCANNNNNNTGG	3 cut(s)	1028, 2088, 2284
BstYI	RGATCY	2 cut(s)	143, 1351
BstZI	CGGCCG	1 cut(s)	168
BsuI	GTATCC	1 cut(s)	1590
BtgI	CCRYGG	5 cut(s)	23, 706, 2283, 2364, 2432
BtgZI	GCGATG	1 cut(s)	2143
BtsI	GCAGTG	4 cut(s)	1158, 1226, 1907, 3030
BtsIMutI	CAGTG	4 cut(s)	1158, 1226, 1907, 3030
BtuMI	TCGCGA	2 cut(s)	160, 260
BveI	ACCTGC	1 cut(s)	1835
Cac8I	GCNNGC	9 cut(s)	58, 418, 519, 1243, 1258, 1372, 2013, 2051, 2826
CaiI	CAGNNNCTG	2 cut(s)	892, 1241
CciI	TCATGA	1 cut(s)	960
Cfr10I	RCCGGY	2 cut(s)	416, 2097
Cfr13I	GGNCC	3 cut(s)	579, 2485, 3098
Cfr42I	CCGCGG	1 cut(s)	26
CseI	GACGC	2 cut(s)	308, 322
Csp6I	GTAC	7 cut(s)	40, 703, 1036, 2373, 2418, 3040, 3334
CspAI	ACCGGT	1 cut(s)	2097
CspCI	CAANNNNNGTGG	2 cut(s)	1593, 1628
CviQI	GTAC	7 cut(s)	40, 703, 1036, 2373, 2418, 3040, 3334
DdeI	CTNAG	9 cut(s)	357, 740, 927, 2026, 2316, 2808, 2953, 3286, 3340
DinI	GGCGCC	1 cut(s)	970
DpnI	GATC	7 cut(s)	145, 237, 284, 362, 458, 1353, 1647
DpnII	GATC	7 cut(s)	143, 235, 282, 360, 456, 1351, 1645
DrdI	GACNNNNNNGTC	1 cut(s)	2098
DseDI	GACNNNNNNGTC	1 cut(s)	2098
EaeI	YGGCCR	3 cut(s)	168, 198, 2177
EagI	CGGCCG	1 cut(s)	168
EciI	GGCGGA	3 cut(s)	580, 823, 1041
Ecl136II	GAGCTC	1 cut(s)	1919
EclXI	CGGCCG	1 cut(s)	168
Eco130I	CCWWGG	5 cut(s)	271, 2283, 2364, 2432, 2533
Eco147I	AGGCCT	1 cut(s)	276
Eco24I	GRGCYC	2 cut(s)	913, 1921
Eco31I	GGTCTC	4 cut(s)	437, 475, 2523, 3287
Eco32I	GATATC	1 cut(s)	1131
Eco47I	GGWCC	1 cut(s)	3098
Eco47III	AGCGCT	1 cut(s)	61
Eco52I	CGGCCG	1 cut(s)	168
Eco53kI	GAGCTC	1 cut(s)	1919
Eco57I	CTGAAG	7 cut(s)	61, 103, 358, 616, 1126, 1218, 3284
Eco88I	CYCGRG	3 cut(s)	524, 749, 3151
EcoICRI	GAGCTC	1 cut(s)	1919
EcoNI	CCTNNNNNAGG	1 cut(s)	901
EcoRI	GAATTC	1 cut(s)	951
EcoRII	CCWGG	9 cut(s)	891, 902, 1149, 1435, 1823, 1938, 2328, 2919, 2946
EcoRV	GATATC	1 cut(s)	1131
EcoT14I	CCWWGG	5 cut(s)	271, 2283, 2364, 2432, 2533
EcoT22I	ATGCAT	2 cut(s)	1501, 2870
EcoT38I	GRGCYC	2 cut(s)	913, 1921
EgeI	GGCGCC	1 cut(s)	970
EheI	GGCGCC	1 cut(s)	970
ErhI	CCWWGG	5 cut(s)	271, 2283, 2364, 2432, 2533
Esp3I	CGTCTC	1 cut(s)	2146
FalI	AAGNNNNNCTT	4 cut(s)	1451, 1483, 2009, 2041
FaqI	GGGAC	6 cut(s)	197, 533, 1363, 1961, 2301, 2990
FauI	CCCGC	2 cut(s)	122, 795
FauNDI	CATATG	2 cut(s)	2864, 3047
FriOI	GRGCYC	2 cut(s)	913, 1921
GsaI	CCCAGC	3 cut(s)	1124, 1706, 2970
GsuI	CTGGAG	5 cut(s)	461, 1122, 1209, 2312, 2654
HaeII	RGCGCY	5 cut(s)	63, 132, 532, 972, 2764
HapII	CCGG	7 cut(s)	11, 251, 318, 417, 2098, 2764, 3101
HgaI	GACGC	2 cut(s)	308, 322
Hin1I	GRCGYC	4 cut(s)	300, 333, 969, 2139
HincII	GTYRAC	1 cut(s)	390
HindII	GTYRAC	1 cut(s)	390
HindIII	AAGCTT	1 cut(s)	2552
HpaII	CCGG	7 cut(s)	11, 251, 318, 417, 2098, 2764, 3101
HphI	GGTGA	2 cut(s)	1084, 1639
Hpy166II	GTNNAC	3 cut(s)	390, 2373, 3040
Hpy188I	TCNGA	9 cut(s)	360, 850, 936, 1135, 1205, 1927, 2104, 2696, 2956
Hpy8I	GTNNAC	3 cut(s)	390, 2373, 3040
Hpy99I	CGWCG	5 cut(s)	206, 260, 302, 385, 599
HpyCH4III	ACNGT	5 cut(s)	1638, 2702, 2848, 2906, 3333
HpyCH4IV	ACGT	1 cut(s)	2139
HpyF3I	CTNAG	9 cut(s)	357, 740, 927, 2026, 2316, 2808, 2953, 3286, 3340
HpySE526I	ACGT	1 cut(s)	2139
Hsp92I	GRCGYC	4 cut(s)	300, 333, 969, 2139
KasI	GGCGCC	1 cut(s)	968
Kpn2I	TCCGGA	1 cut(s)	3100
KroI	GCCGGC	1 cut(s)	416
KroNI	GCCGGC	1 cut(s)	418
KspI	CCGCGG	1 cut(s)	26
Kzo9I	GATC	7 cut(s)	143, 235, 282, 360, 456, 1351, 1645
LguI	GCTCTTC	2 cut(s)	869, 1909
LweI	GCATC	6 cut(s)	768, 1126, 1806, 2012, 2811, 2877
MaeII	ACGT	1 cut(s)	2139
MalI	GATC	7 cut(s)	145, 237, 284, 362, 458, 1353, 1647
MboI	GATC	7 cut(s)	143, 235, 282, 360, 456, 1351, 1645
MfeI	CAATTG	1 cut(s)	510
MflI	RGATCY	2 cut(s)	143, 1351
MhlI	GDGCHC	3 cut(s)	519, 913, 1921
MlsI	TGGCCA	1 cut(s)	2179
MluNI	TGGCCA	1 cut(s)	2179
Mly113I	GGCGCC	1 cut(s)	969
MlyI	GAGTC	4 cut(s)	104, 1020, 2945, 3197
MmeI	TCCRAC	1 cut(s)	1183
Mox20I	TGGCCA	1 cut(s)	2179
Mph1103I	ATGCAT	2 cut(s)	1501, 2870
MroI	TCCGGA	1 cut(s)	3100
MroNI	GCCGGC	1 cut(s)	416
MroXI	GAANNNNTTC	1 cut(s)	1388
MscI	TGGCCA	1 cut(s)	2179
MseI	TTAA	6 cut(s)	1533, 2112, 2499, 2876, 3188, 3231
MslI	CAYNNNNRTG	5 cut(s)	1043, 1845, 2282, 3035, 3069
Msp20I	TGGCCA	1 cut(s)	2179
MspA1I	CMGCKG	6 cut(s)	25, 636, 943, 1100, 1748, 2336
MspI	CCGG	7 cut(s)	11, 251, 318, 417, 2098, 2764, 3101
MunI	CAATTG	1 cut(s)	510
Mva1269I	GAATGC	1 cut(s)	2505
MvaI	CCWGG	9 cut(s)	893, 904, 1151, 1437, 1825, 1940, 2330, 2921, 2948
MvnI	CGCG	5 cut(s)	25, 160, 182, 206, 260
NaeI	GCCGGC	1 cut(s)	418
NarI	GGCGCC	1 cut(s)	969
NciI	CCSGG	1 cut(s)	2765
NcoI	CCATGG	3 cut(s)	2283, 2364, 2432
NdeI	CATATG	2 cut(s)	2864, 3047
NdeII	GATC	7 cut(s)	143, 235, 282, 360, 456, 1351, 1645
NgoMIV	GCCGGC	1 cut(s)	416
NlaIV	GGNNCC	9 cut(s)	88, 322, 720, 910, 970, 1047, 1111, 1313, 2925
NmeAIII	GCCGAG	2 cut(s)	219, 466
NmuCI	GTSAC	5 cut(s)	922, 1072, 1489, 1600, 2467
NruI	TCGCGA	2 cut(s)	160, 260
NsiI	ATGCAT	2 cut(s)	1501, 2870
NspI	RCATGY	3 cut(s)	1378, 1686, 2348
NspV	TTCGAA	1 cut(s)	1457
OliI	CACNNNNGTG	2 cut(s)	1845, 3035
PaeR7I	CTCGAG	2 cut(s)	524, 749
PagI	TCATGA	1 cut(s)	960
PaqCI	CACCTGC	1 cut(s)	1835
PceI	AGGCCT	1 cut(s)	276
PciI	ACATGT	1 cut(s)	1682
PciSI	GCTCTTC	2 cut(s)	869, 1909
PctI	GAATGC	1 cut(s)	2505
PdiI	GCCGGC	1 cut(s)	418
PdmI	GAANNNNTTC	1 cut(s)	1388
PfeI	GAWTC	8 cut(s)	162, 409, 490, 502, 695, 931, 2691, 2748
PflFI	GACNNNGTC	1 cut(s)	2700
PflMI	CCANNNNNTGG	2 cut(s)	892, 2831
PinAI	ACCGGT	1 cut(s)	2097
PleI	GAGTC	4 cut(s)	104, 1019, 2945, 3197
PluTI	GGCGCC	1 cut(s)	972
PpsI	GAGTC	4 cut(s)	104, 1019, 2945, 3197
PscI	ACATGT	1 cut(s)	1682
Psp124BI	GAGCTC	1 cut(s)	1921
Psp6I	CCWGG	9 cut(s)	891, 902, 1149, 1435, 1823, 1938, 2328, 2919, 2946
PspFI	CCCAGC	3 cut(s)	1120, 1702, 2966
PspGI	CCWGG	9 cut(s)	891, 902, 1149, 1435, 1823, 1938, 2328, 2919, 2946
PspN4I	GGNNCC	9 cut(s)	88, 322, 720, 910, 970, 1047, 1111, 1313, 2925
PspPI	GGNCC	3 cut(s)	579, 2485, 3098
PspXI	VCTCGAGB	1 cut(s)	524
PsrI	GAACNNNNNNTAC	2 cut(s)	764, 796
PstI	CTGCAG	1 cut(s)	21
PstNI	CAGNNNCTG	2 cut(s)	892, 1241
PsuI	RGATCY	2 cut(s)	143, 1351
PsyI	GACNNNGTC	1 cut(s)	2700
PvuII	CAGCTG	5 cut(s)	636, 943, 1100, 1748, 2336
RruI	TCGCGA	2 cut(s)	160, 260
RsaI	GTAC	7 cut(s)	41, 704, 1037, 2374, 2419, 3041, 3335
RsaNI	GTAC	7 cut(s)	40, 703, 1036, 2373, 2418, 3040, 3334
RseI	CAYNNNNRTG	5 cut(s)	1043, 1845, 2282, 3035, 3069
SacI	GAGCTC	1 cut(s)	1921
SacII	CCGCGG	1 cut(s)	26
SapI	GCTCTTC	2 cut(s)	869, 1909
SaqAI	TTAA	6 cut(s)	1533, 2112, 2499, 2876, 3188, 3231
Sau3AI	GATC	7 cut(s)	143, 235, 282, 360, 456, 1351, 1645
Sau96I	GGNCC	3 cut(s)	579, 2485, 3098
SchI	GAGTC	4 cut(s)	104, 1020, 2945, 3197
SduI	GDGCHC	3 cut(s)	519, 913, 1921
SfaNI	GCATC	6 cut(s)	768, 1126, 1806, 2012, 2811, 2877
SfcI	CTRYAG	1 cut(s)	17
SfoI	GGCGCC	1 cut(s)	970
Sfr274I	CTCGAG	2 cut(s)	524, 749
Sfr303I	CCGCGG	1 cut(s)	26
SfuI	TTCGAA	1 cut(s)	1457
SgrBI	CCGCGG	1 cut(s)	26
SinI	GGWCC	1 cut(s)	3098
SlaI	CTCGAG	2 cut(s)	524, 749
SmiMI	CAYNNNNRTG	5 cut(s)	1043, 1845, 2282, 3035, 3069
SmlI	CTYRAG	4 cut(s)	149, 524, 749, 2413
SmoI	CTYRAG	4 cut(s)	149, 524, 749, 2413
SpeI	ACTAGT	1 cut(s)	2449
SseBI	AGGCCT	1 cut(s)	276
SspDI	GGCGCC	1 cut(s)	968
SspI	AATATT	2 cut(s)	1360, 2913
SstI	GAGCTC	1 cut(s)	1921
StuI	AGGCCT	1 cut(s)	276
StyI	CCWWGG	5 cut(s)	271, 2283, 2364, 2432, 2533
TaaI	ACNGT	5 cut(s)	1638, 2702, 2848, 2906, 3333
TaiI	ACGT	1 cut(s)	2142
TaqI	TCGA	7 cut(s)	234, 297, 498, 525, 750, 1457, 1651
TaqII	GACCGA	1 cut(s)	461
TatI	WGTACW	2 cut(s)	3039, 3333
TfiI	GAWTC	8 cut(s)	162, 409, 490, 502, 695, 931, 2691, 2748
Tru1I	TTAA	6 cut(s)	1533, 2112, 2499, 2876, 3188, 3231
Tru9I	TTAA	6 cut(s)	1533, 2112, 2499, 2876, 3188, 3231
TscAI	CASTG	4 cut(s)	1165, 1233, 1907, 3037
TseFI	GTSAC	5 cut(s)	922, 1072, 1489, 1600, 2467
Tsp45I	GTSAC	5 cut(s)	922, 1072, 1489, 1600, 2467
TspDTI	ATGAA	8 cut(s)	859, 977, 1014, 1710, 2146, 2608, 2661, 2740
TspGWI	ACGGA	2 cut(s)	23, 27
TspRI	CASTG	4 cut(s)	1165, 1233, 1907, 3037
Tth111I	GACNNNGTC	1 cut(s)	2700
Van91I	CCANNNNNTGG	2 cut(s)	892, 2831
VpaK11BI	GGWCC	1 cut(s)	3098
XagI	CCTNNNNNAGG	1 cut(s)	901
XapI	RAATTY	3 cut(s)	730, 951, 3124
XbaI	TCTAGA	2 cut(s)	140, 3323
XceI	RCATGY	3 cut(s)	1378, 1686, 2348
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2172
XhoI	CTCGAG	2 cut(s)	524, 749
XmaJI	CCTAGG	2 cut(s)	271, 2533
XmnI	GAANNNNTTC	1 cut(s)	1388
ZraI	GACGTC	1 cut(s)	2140
Zsp2I	ATGCAT	2 cut(s)	1501, 2870

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.