Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0003028

Physical Location & Seq

Genomic Coordinates Forward (+)

26469652 .. 26474115

Gene Structure 4464 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g24220.t7

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2163 bp

ATGGGGTTTTCAGGGTTGTTCAAGAGGTTTAGCAGTCTGTACAAACCCAATGCCAGAATCCAAGCATACCCATCTCCCTACATGCCCGGAAGAAGAGCCTTATCAAGCTCTAAGCTTTTCAACAATGGCGACGATTCTGTGCTTCCGGTTCTGATAGTGGGTGCAGGACCTGTGGGTCTCGTTCTTTCCATACTCTTAACAAAGCTAGGGGTCAAATGCGCGGTTTTGGAGAAAAGTACAAGTTTCTCCAGACATCCACAGGCACATTTCATTAACAATCGGTCGATGGAGATATTTCGCAAATTGGACGGCCTGGCAGAGGAGATCCAGAGGTCTCAACCACCAGTAGATTTGTGGAGAAAGTTTATATATTGCACTTCGCTCTCCGGTTCCATTCTTGGGTCAGTGGATCATATGCAACCTCAAGATTTTGAGAAAGTTGTCAGCCCAGTCTCTGTTGCACACTTCTCTCAGTACAAAGTAAATGCATTACTACTTAAGCAGCTTGAAAATCTCAGCTTCAAGTTTTGTACGCCTGAAGGGTTGAAATGCCTTGACCATGAAGCCTTTGAGGCAAGACAAATATTGATAGGCCATGAATGTGTTTCCATCAACGCCAGTGATGATATTGTTTCTGTCACCGCATCTTTTCTGAAGGATGGAAAACATATGGAGAGGAATATCAGATGCAATATGGTTGTTGGTACAGATGGTGCTGGAAGTACTGTGCGTAAGCTGGCTGGTATAGATTTGAGAGGTGAAAAGGACTTGCAGAAGCTTGTGAGTGTCCATTTCTTGAGCAGAGACCTTGGAAATTACTTGCTCAGTGAGAGACCTGGCATGCTGTTTTTTATCTTCAATACTGAAGCTATTGGGGTCCTTGTTGCACATGATCTCCAGCAAGGGGAATTTGTATTGCAGATACCATTTTATCCACCCCAGCAAAACCTTGAAGATTTCAGTCCCAAGACATGTGAGAGGATAATCTTCAAATTGGTAGGTCGAGAGCTTGGAGACGTAAATGTGATTGACATCAAACCTTGGGTGATGCATGCTGAAGTTGCCGAGAAGTTTCTGAGTAGTCAAAACCGTATAATACTTGCTGGTGATGCTGCTCATCGATTCCCTCCTGCTGGTGGATTTGGCATGAATACTGGAATTCAGGATTCTCATAACCTTGCCTGGAAGATAGCTTCTGTTGTAAAGGGTATTGCACCACCTTCAATACTTAATACTTATGAAACAGAACGTAGGCCGATTGCCATTTTCAATACAAATCTTAGTATTCAAAACTTCAAAGCAGCCATGGCTGTTCCTGGTGCACTTGGTCTTGATCCAACAATTGCAAACTCGGTACATCAACTAATAAATAAGGGGGTTGGATCCATTTTACCATCTGGAGTTCAGAGCGCCATTTTGGATGGTATTTTCACTTTAGGTCGTGCACAGTTGTCAGAAACTCTTTTGAATGAAAACAACCCACTTGGAGCGTTAAGGCTTGCTAATCTGAGACGCATATTTGAAGAGGGAAAGAGTCTCCAGCTTCAGTTCCCAGCTGAGGATCTTGGTTTCAGGTACCCAGACGGTGCACTTATTCCTGATCATGATGGTGCACAGGGTAGTCCAGATGAACCTACTGGTTGCCGGAGGGACTATGTTCCTACGGCAGATCCAGGTTCGAGGCTCCCTCACATGAATGTGAGGATATTGTCAAATTCATCGAGTGAGGCAACTATTTCTACTCTGGATCTCATATGTGGAGACAGAGTTGAGTTTCTCCTTATTATAGCACCAATGGAGCTGTCCTACCATGTTGCTCATGCTGCATTGAAGGTGGCTGAGGAATTCAAGGTTTCTGCAAGGGTGTGCGTGTTGTGGCCTGCTGGTAGTGCTGAAGGCATTGAGGTAGGAAGTAAGGCATCATTGAAACCGTGGGAGAACTACATAGATATTATGGAAGTTAAAAAGTCATCAAATGCTTCGTCATGGTGGGATATCTGTCAAATGAGTGATGAAGGAGCCATCCTAGTTCGGCCTGATGAGCATGTTGCTTGGCGTGTGAAGTCTGGTGTTGTTGGGAACCCTATAAGGGAAATGAGAAGGGCTTTTTCTGCTGCCTTAGGCTTGAAGTCACCAAATAGAGACCAAGAAGCTGGTCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_3

Protein Analysis

721

Amino Acids

79.05

Weight (kDa)

6.67

Isoelectric Point (pI)

40.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_3	PF01494	53 - 283	2.2e-48	FAD binding domain
Rng_hyd_C	PF21274	501 - 569	7.8e-07	Aromatic-ring hydroxylase, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003028)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24340
fragaria_vesca	FvH4_4g24220 FvH4_4g24220 FvH4_4g24220 FvH4_4g24220 FvH4_4g24220 FvH4_4g24220 FvH4_4g24220 FvH4_4g24220 FvH4_4g24220 FvH4_4g24220
malus_domestica	MD13G1113200.v1.1 MD16G1112300.v1.1
prunus_persica	Prupe.1G232900_v2.0.a1
pyrus_communis	pycom13g09860
rosa_chinensis	RchiOBHm_Chr4g0431061
rosa_laevigata	RLG00000006928 RLG00000016399
rosa_multiflora	Rmu_co8265599.1_g000001 Rmu_sc0003592.1_g000008 Rmu_sc0010203.1_g000008 Rmu_sc0041255.1_g000001 Rmu_sc0043112.1_g000001
rosa_roxburghii	Rroxscaffold_2G00114870 Rroxscaffold_2G00148110 Rroxscaffold_5G00372680
rosa_rugosa	Rorug02G0035800 Rorug02G0035800 Rorug02G0035900 Rorug02G0035900 Rorug04G0247900
rosa_samantha	Rh2DG080900 Rh2DG081100 Rh4AG304600 Rh4BG311800 Rh4CG328100 Rh4DG308300
rosa_wichuraiana	Rw4G026450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	174
Acc65I	GGTACC	1 cut(s)	1575
AccB1I	GGYRCC	1 cut(s)	1575
AccII	CGCG	1 cut(s)	221
AciI	CCGC	2 cut(s)	221, 642
AclWI	GGATC	8 cut(s)	319, 417, 1328, 1377, 1390, 1569, 1664, 1755
AcsI	RAATTY	4 cut(s)	908, 1158, 1714, 1844
AcuI	CTGAAG	6 cut(s)	558, 674, 885, 1077, 1529, 1914
AfaI	GTAC	8 cut(s)	41, 238, 476, 532, 706, 724, 1356, 1577
AfiI	CCNNNNNNNGG	7 cut(s)	319, 399, 904, 1133, 1136, 1558, 2089
AflII	CTTAAG	1 cut(s)	497
AflIII	ACRYGT	1 cut(s)	971
AjnI	CCWGG	5 cut(s)	312, 835, 1181, 1315, 1672
Alw21I	GWGCWC	4 cut(s)	1324, 1447, 1591, 1615
Alw44I	GTGCAC	4 cut(s)	1320, 1443, 1587, 1611
AlwI	GGATC	8 cut(s)	319, 417, 1328, 1377, 1390, 1569, 1664, 1755
AlwNI	CAGNNNCTG	2 cut(s)	170, 455
AoxI	GGCC	5 cut(s)	310, 592, 1253, 1877, 2033
ApaLI	GTGCAC	4 cut(s)	1320, 1443, 1587, 1611
ApeKI	GCWGC	5 cut(s)	502, 1112, 1301, 1823, 2114
ApoI	RAATTY	4 cut(s)	908, 1158, 1714, 1844
ArsI	GACNNNNNNTTYG	2 cut(s)	1967, 1999
Asp718I	GGTACC	1 cut(s)	1575
AspLEI	GCGC	2 cut(s)	221, 1412
AspS9I	GGNCC	2 cut(s)	167, 877
AsuC2I	CCSGG	1 cut(s)	87
AsuHPI	GGTGA	5 cut(s)	631, 770, 1057, 1118, 2124
AvaII	GGWCC	2 cut(s)	167, 877
AxyI	CCTNAGG	1 cut(s)	2119
BaeGI	GKGCMC	4 cut(s)	1324, 1447, 1591, 1615
BamHI	GGATCC	1 cut(s)	1382
BanI	GGYRCC	1 cut(s)	1575
Bbv12I	GWGCWC	4 cut(s)	1324, 1447, 1591, 1615
BbvCI	CCTCAGC	2 cut(s)	1557, 1839
BbvI	GCAGC	5 cut(s)	514, 1099, 1313, 1810, 2101
BccI	CCATC	9 cut(s)	79, 280, 617, 653, 704, 1402, 1415, 1601, 2030
BceAI	ACGGC	2 cut(s)	325, 1680
BciT130I	CCWGG	5 cut(s)	314, 837, 1183, 1317, 1674
BclI	TGATCA	1 cut(s)	1600
BcnI	CCSGG	1 cut(s)	87
BfaI	CTAG	2 cut(s)	206, 2027
BfoI	RGCGCY	1 cut(s)	1413
BfrI	CTTAAG	1 cut(s)	497
BglI	GCCNNNNNGGC	1 cut(s)	572
BisI	GCNGC	5 cut(s)	503, 1113, 1302, 1824, 2115
BlsI	GCNGC	5 cut(s)	504, 1114, 1303, 1825, 2116
BmcAI	AGTACT	1 cut(s)	724
Bme1390I	CCNGG	6 cut(s)	87, 314, 837, 1183, 1317, 1674
Bme18I	GGWCC	2 cut(s)	167, 877
BmgT120I	GGNCC	2 cut(s)	167, 877
BmiI	GGNNCC	7 cut(s)	391, 878, 1384, 1577, 1685, 2020, 2081
BmrFI	CCNGG	6 cut(s)	87, 314, 837, 1183, 1317, 1674
BmrI	ACTGGG	1 cut(s)	443
BmsI	GCATC	5 cut(s)	653, 677, 1038, 1099, 1928
BmuI	ACTGGG	1 cut(s)	443
BplI	GAGNNNNNCTC	2 cut(s)	1672, 1704
BpmI	CTGGAG	4 cut(s)	232, 881, 1419, 1523
Bpu10I	CCTNAGC	2 cut(s)	1557, 1839
BpuEI	CTTGAG	2 cut(s)	408, 817
BpuMI	CCSGG	1 cut(s)	87
Bsa29I	ATCGAT	1 cut(s)	1120
BsaBI	GATNNNNATC	1 cut(s)	1031
BsaI	GGTCTC	5 cut(s)	182, 339, 798, 826, 2136
BsaJI	CCNNGG	4 cut(s)	808, 1040, 1305, 1931
BsaWI	WCCGGW	2 cut(s)	145, 386
BsaXI	ACNNNNNCTCC	4 cut(s)	879, 909, 1752, 1782
Bsc4I	CCNNNNNNNGG	7 cut(s)	319, 399, 904, 1133, 1136, 1558, 2089
Bse1I	ACTGG	5 cut(s)	344, 449, 618, 1159, 1642
Bse21I	CCTNAGG	1 cut(s)	2119
Bse8I	GATNNNNATC	1 cut(s)	1031
BseBI	CCWGG	5 cut(s)	314, 837, 1183, 1317, 1674
BseCI	ATCGAT	1 cut(s)	1120
BseDI	CCNNGG	4 cut(s)	808, 1040, 1305, 1931
BseGI	GGATG	4 cut(s)	253, 664, 1426, 2022
BseJI	GATNNNNATC	1 cut(s)	1031
BseLI	CCNNNNNNNGG	7 cut(s)	319, 399, 904, 1133, 1136, 1558, 2089
BseMII	CTCAG	7 cut(s)	485, 529, 838, 1067, 1499, 1548, 1830
BseNI	ACTGG	5 cut(s)	344, 449, 618, 1159, 1642
BseRI	GAGGAG	1 cut(s)	335
BseSI	GKGCMC	4 cut(s)	1324, 1447, 1591, 1615
BseXI	GCAGC	5 cut(s)	514, 1099, 1313, 1810, 2101
BseYI	CCCAGC	2 cut(s)	939, 1552
BsgI	GTGCAG	1 cut(s)	183
Bsh1236I	CGCG	1 cut(s)	221
Bsh1285I	CGRYCG	1 cut(s)	284
BshFI	GGCC	5 cut(s)	312, 594, 1255, 1879, 2035
BshNI	GGYRCC	1 cut(s)	1575
BshVI	ATCGAT	1 cut(s)	1120
BsiEI	CGRYCG	1 cut(s)	284
BsiHKAI	GWGCWC	4 cut(s)	1324, 1447, 1591, 1615
BsiSI	CCGG	4 cut(s)	87, 146, 387, 1645
BslFI	GGGAC	2 cut(s)	948, 1664
BslI	CCNNNNNNNGG	7 cut(s)	319, 399, 904, 1133, 1136, 1558, 2089
BsmBI	CGTCTC	2 cut(s)	1008, 1504
BsmFI	GGGAC	2 cut(s)	948, 1664
BsnI	GGCC	5 cut(s)	312, 594, 1255, 1879, 2035
Bso31I	GGTCTC	5 cut(s)	182, 339, 798, 826, 2136
Bsp1286I	GDGCHC	4 cut(s)	1324, 1447, 1591, 1615
Bsp1407I	TGTACA	1 cut(s)	39
Bsp143I	GATC	9 cut(s)	324, 409, 892, 1333, 1382, 1561, 1600, 1669, 1747
Bsp19I	CCATGG	1 cut(s)	1305
BspACI	CCGC	2 cut(s)	221, 642
BspANI	GGCC	5 cut(s)	312, 594, 1255, 1879, 2035
BspCNI	CTCAG	7 cut(s)	484, 528, 837, 1068, 1500, 1549, 1831
BspDI	ATCGAT	1 cut(s)	1120
BspFNI	CGCG	1 cut(s)	221
BspHI	TCATGA	1 cut(s)	1603
BspLI	GGNNCC	7 cut(s)	391, 878, 1384, 1577, 1685, 2020, 2081
BspPI	GGATC	8 cut(s)	319, 417, 1328, 1377, 1390, 1569, 1664, 1755
BspQI	GCTCTTC	1 cut(s)	88
BspT107I	GGYRCC	1 cut(s)	1575
BspTI	CTTAAG	1 cut(s)	497
BspTNI	GGTCTC	5 cut(s)	182, 339, 798, 826, 2136
BsrGI	TGTACA	1 cut(s)	39
BsrI	ACTGG	5 cut(s)	344, 449, 618, 1159, 1642
BssECI	CCNNGG	4 cut(s)	808, 1040, 1305, 1931
BssMI	GATC	9 cut(s)	324, 409, 892, 1333, 1382, 1561, 1600, 1669, 1747
BssT1I	CCWWGG	3 cut(s)	808, 1040, 1305
Bst2UI	CCWGG	5 cut(s)	314, 837, 1183, 1317, 1674
Bst4CI	ACNGT	5 cut(s)	727, 1091, 1449, 1586, 1932
Bst6I	CTCTTC	2 cut(s)	88, 1518
BstAFI	CTTAAG	1 cut(s)	497
BstAUI	TGTACA	1 cut(s)	39
BstC8I	GCNNGC	5 cut(s)	738, 842, 1053, 1500, 1881
BstDSI	CCRYGG	2 cut(s)	1305, 1931
BstENI	CCTNNNNNAGG	1 cut(s)	317
BstF5I	GGATG	4 cut(s)	253, 664, 1426, 2022
BstFNI	CGCG	1 cut(s)	221
BstH2I	RGCGCY	1 cut(s)	1413
BstHHI	GCGC	2 cut(s)	221, 1412
BstKTI	GATC	9 cut(s)	327, 412, 895, 1336, 1385, 1564, 1603, 1672, 1750
BstMBI	GATC	9 cut(s)	324, 409, 892, 1333, 1382, 1561, 1600, 1669, 1747
BstMCI	CGRYCG	1 cut(s)	284
BstMWI	GCNNNNNNNGC	8 cut(s)	572, 1061, 1109, 1307, 1823, 1889, 2041, 2111
BstNI	CCWGG	5 cut(s)	314, 837, 1183, 1317, 1674
BstNSI	RCATGY	5 cut(s)	85, 844, 975, 1055, 2048
BstSCI	CCNGG	6 cut(s)	85, 312, 835, 1181, 1315, 1672
BstSLI	GKGCMC	4 cut(s)	1324, 1447, 1591, 1615
BstUI	CGCG	1 cut(s)	221
BstV1I	GCAGC	5 cut(s)	514, 1099, 1313, 1810, 2101
BstX2I	RGATCY	5 cut(s)	324, 1382, 1561, 1669, 1747
BstXI	CCANNNNNNTGG	1 cut(s)	2153
BstYI	RGATCY	5 cut(s)	324, 1382, 1561, 1669, 1747
Bsu15I	ATCGAT	1 cut(s)	1120
Bsu36I	CCTNAGG	1 cut(s)	2119
BsuRI	GGCC	5 cut(s)	312, 594, 1255, 1879, 2035
BsuTUI	ATCGAT	1 cut(s)	1120
BtgI	CCRYGG	2 cut(s)	1305, 1931
BtsCI	GGATG	4 cut(s)	253, 664, 1426, 2022
BtsIMutI	CAGTG	3 cut(s)	411, 625, 832
Cac8I	GCNNGC	5 cut(s)	738, 842, 1053, 1500, 1881
CaiI	CAGNNNCTG	2 cut(s)	170, 455
CciI	TCATGA	1 cut(s)	1603
CfoI	GCGC	2 cut(s)	221, 1412
Cfr13I	GGNCC	2 cut(s)	167, 877
ClaI	ATCGAT	1 cut(s)	1120
CseI	GACGC	1 cut(s)	1521
Csp6I	GTAC	8 cut(s)	40, 237, 475, 531, 705, 723, 1355, 1576
CviQI	GTAC	8 cut(s)	40, 237, 475, 531, 705, 723, 1355, 1576
DpnI	GATC	9 cut(s)	326, 411, 894, 1335, 1384, 1563, 1602, 1671, 1749
DpnII	GATC	9 cut(s)	324, 409, 892, 1333, 1382, 1561, 1600, 1669, 1747
DrdI	GACNNNNNNGTC	1 cut(s)	174
DseDI	GACNNNNNNGTC	1 cut(s)	174
Eam1104I	CTCTTC	2 cut(s)	88, 1518
EarI	CTCTTC	2 cut(s)	88, 1518
Eco130I	CCWWGG	3 cut(s)	808, 1040, 1305
Eco31I	GGTCTC	5 cut(s)	182, 339, 798, 826, 2136
Eco32I	GATATC	1 cut(s)	1996
Eco47I	GGWCC	2 cut(s)	167, 877
Eco57I	CTGAAG	6 cut(s)	558, 674, 885, 1077, 1529, 1914
Eco81I	CCTNAGG	1 cut(s)	2119
EcoNI	CCTNNNNNAGG	1 cut(s)	317
EcoO109I	RGGNCCY	2 cut(s)	167, 877
EcoRI	GAATTC	2 cut(s)	1158, 1844
EcoRII	CCWGG	5 cut(s)	312, 835, 1181, 1315, 1672
EcoRV	GATATC	1 cut(s)	1996
EcoT14I	CCWWGG	3 cut(s)	808, 1040, 1305
EcoT22I	ATGCAT	2 cut(s)	490, 1053
ErhI	CCWWGG	3 cut(s)	808, 1040, 1305
Esp3I	CGTCTC	2 cut(s)	1008, 1504
FaqI	GGGAC	2 cut(s)	948, 1664
FauNDI	CATATG	3 cut(s)	414, 669, 1754
FbaI	TGATCA	1 cut(s)	1600
Fnu4HI	GCNGC	5 cut(s)	503, 1113, 1302, 1824, 2115
FokI	GGATG	4 cut(s)	240, 671, 1433, 2009
Fsp4HI	GCNGC	5 cut(s)	503, 1113, 1302, 1824, 2115
FspBI	CTAG	2 cut(s)	206, 2027
GlaI	GCGC	2 cut(s)	220, 1411
GluI	GCNGC	5 cut(s)	503, 1113, 1302, 1824, 2115
GsaI	CCCAGC	2 cut(s)	943, 1556
GsuI	CTGGAG	4 cut(s)	232, 881, 1419, 1523
HaeII	RGCGCY	1 cut(s)	1413
HaeIII	GGCC	5 cut(s)	312, 594, 1255, 1879, 2035
HapII	CCGG	4 cut(s)	87, 146, 387, 1645
HgaI	GACGC	1 cut(s)	1521
HhaI	GCGC	2 cut(s)	221, 1412
Hin6I	GCGC	2 cut(s)	219, 1410
HinP1I	GCGC	2 cut(s)	219, 1410
HindIII	AAGCTT	2 cut(s)	113, 776
HinfI	GANTC	5 cut(s)	57, 134, 1122, 1166, 1534
HpaII	CCGG	4 cut(s)	87, 146, 387, 1645
HphI	GGTGA	5 cut(s)	631, 770, 1057, 1118, 2124
Hpy166II	GTNNAC	4 cut(s)	1322, 1445, 1589, 1613
Hpy188I	TCNGA	7 cut(s)	153, 654, 686, 1077, 1407, 1456, 1509
Hpy8I	GTNNAC	4 cut(s)	1322, 1445, 1589, 1613
Hpy99I	CGWCG	1 cut(s)	134
HpyAV	CCTTC	7 cut(s)	533, 649, 1230, 1825, 1889, 2009, 2094
HpyCH4III	ACNGT	5 cut(s)	727, 1091, 1449, 1586, 1932
HpyCH4IV	ACGT	2 cut(s)	1017, 1249
HpyF10VI	GCNNNNNNNGC	8 cut(s)	572, 1061, 1109, 1307, 1823, 1889, 2041, 2111
HpySE526I	ACGT	2 cut(s)	1017, 1249
HspAI	GCGC	2 cut(s)	219, 1410
KpnI	GGTACC	1 cut(s)	1579
Ksp22I	TGATCA	1 cut(s)	1600
Kzo9I	GATC	9 cut(s)	324, 409, 892, 1333, 1382, 1561, 1600, 1669, 1747
LguI	GCTCTTC	1 cut(s)	88
LmnI	GCTCC	4 cut(s)	1487, 1689, 1798, 2018
Lsp1109I	GCAGC	5 cut(s)	514, 1099, 1313, 1810, 2101
LweI	GCATC	5 cut(s)	653, 677, 1038, 1099, 1928
MaeI	CTAG	2 cut(s)	206, 2027
MaeII	ACGT	2 cut(s)	1017, 1249
MaeIII	GTNAC	2 cut(s)	637, 2130
MalI	GATC	9 cut(s)	326, 411, 894, 1335, 1384, 1563, 1602, 1671, 1749
MboI	GATC	9 cut(s)	324, 409, 892, 1333, 1382, 1561, 1600, 1669, 1747
MboII	GAAGA	7 cut(s)	102, 105, 847, 965, 979, 1198, 1535
MfeI	CAATTG	1 cut(s)	1341
MflI	RGATCY	5 cut(s)	324, 1382, 1561, 1669, 1747
MhlI	GDGCHC	4 cut(s)	1324, 1447, 1591, 1615
MluCI	AATT	8 cut(s)	302, 814, 908, 992, 1158, 1341, 1714, 1844
MlyI	GAGTC	1 cut(s)	1543
MmeI	TCCRAC	2 cut(s)	1360, 1361
Mph1103I	ATGCAT	2 cut(s)	490, 1053
MseI	TTAA	6 cut(s)	197, 273, 498, 1230, 1493, 1962
MslI	CAYNNNNRTG	4 cut(s)	600, 1608, 1695, 1697
MspA1I	CMGCKG	1 cut(s)	1556
MspCI	CTTAAG	1 cut(s)	497
MspI	CCGG	4 cut(s)	87, 146, 387, 1645
MspR9I	CCNGG	6 cut(s)	87, 314, 837, 1183, 1317, 1674
MunI	CAATTG	1 cut(s)	1341
MvaI	CCWGG	5 cut(s)	314, 837, 1183, 1317, 1674
MvnI	CGCG	1 cut(s)	221
MwoI	GCNNNNNNNGC	8 cut(s)	572, 1061, 1109, 1307, 1823, 1889, 2041, 2111
NciI	CCSGG	1 cut(s)	87
NcoI	CCATGG	1 cut(s)	1305
NdeI	CATATG	3 cut(s)	414, 669, 1754
NdeII	GATC	9 cut(s)	324, 409, 892, 1333, 1382, 1561, 1600, 1669, 1747
NlaIV	GGNNCC	7 cut(s)	391, 878, 1384, 1577, 1685, 2020, 2081
NmeAIII	GCCGAG	1 cut(s)	1090
NmuCI	GTSAC	2 cut(s)	637, 2130
NsiI	ATGCAT	2 cut(s)	490, 1053
NspI	RCATGY	5 cut(s)	85, 844, 975, 1055, 2048
PaeI	GCATGC	2 cut(s)	844, 1055
PagI	TCATGA	1 cut(s)	1603
PciI	ACATGT	1 cut(s)	971
PciSI	GCTCTTC	1 cut(s)	88
PfeI	GAWTC	4 cut(s)	57, 134, 1122, 1166
PkrI	GCNGC	5 cut(s)	504, 1114, 1303, 1825, 2116
PleI	GAGTC	1 cut(s)	1542
PpsI	GAGTC	1 cut(s)	1542
PpuMI	RGGWCCY	2 cut(s)	167, 877
PscI	ACATGT	1 cut(s)	971
Psp5II	RGGWCCY	2 cut(s)	167, 877
Psp6I	CCWGG	5 cut(s)	312, 835, 1181, 1315, 1672
PspFI	CCCAGC	2 cut(s)	939, 1552
PspGI	CCWGG	5 cut(s)	312, 835, 1181, 1315, 1672
PspN4I	GGNNCC	7 cut(s)	391, 878, 1384, 1577, 1685, 2020, 2081
PspPI	GGNCC	2 cut(s)	167, 877
PspPPI	RGGWCCY	2 cut(s)	167, 877
PstNI	CAGNNNCTG	2 cut(s)	170, 455
PsuI	RGATCY	5 cut(s)	324, 1382, 1561, 1669, 1747
PvuII	CAGCTG	1 cut(s)	1556
RsaI	GTAC	8 cut(s)	41, 238, 476, 532, 706, 724, 1356, 1577
RsaNI	GTAC	8 cut(s)	40, 237, 475, 531, 705, 723, 1355, 1576
RseI	CAYNNNNRTG	4 cut(s)	600, 1608, 1695, 1697
SapI	GCTCTTC	1 cut(s)	88
SaqAI	TTAA	6 cut(s)	197, 273, 498, 1230, 1493, 1962
SatI	GCNGC	5 cut(s)	503, 1113, 1302, 1824, 2115
Sau3AI	GATC	9 cut(s)	324, 409, 892, 1333, 1382, 1561, 1600, 1669, 1747
Sau96I	GGNCC	2 cut(s)	167, 877
ScaI	AGTACT	1 cut(s)	724
SchI	GAGTC	1 cut(s)	1543
ScrFI	CCNGG	6 cut(s)	87, 314, 837, 1183, 1317, 1674
SduI	GDGCHC	4 cut(s)	1324, 1447, 1591, 1615
SfaNI	GCATC	5 cut(s)	653, 677, 1038, 1099, 1928
SinI	GGWCC	2 cut(s)	167, 877
SmiMI	CAYNNNNRTG	4 cut(s)	600, 1608, 1695, 1697
SmlI	CTYRAG	3 cut(s)	423, 497, 796
SmoI	CTYRAG	3 cut(s)	423, 497, 796
SphI	GCATGC	2 cut(s)	844, 1055
Sse9I	AATT	8 cut(s)	302, 814, 908, 992, 1158, 1341, 1714, 1844
SsiI	CCGC	2 cut(s)	221, 642
SspI	AATATT	1 cut(s)	585
SspMI	CTAG	2 cut(s)	206, 2027
StyD4I	CCNGG	6 cut(s)	85, 312, 835, 1181, 1315, 1672
StyI	CCWWGG	3 cut(s)	808, 1040, 1305
TaaI	ACNGT	5 cut(s)	727, 1091, 1449, 1586, 1932
TaiI	ACGT	2 cut(s)	1020, 1252
TaqI	TCGA	5 cut(s)	284, 1003, 1120, 1679, 1721
TaqII	GACCGA	1 cut(s)	270
TasI	AATT	8 cut(s)	302, 814, 908, 992, 1158, 1341, 1714, 1844
TatI	WGTACW	4 cut(s)	39, 236, 474, 722
TfiI	GAWTC	4 cut(s)	57, 134, 1122, 1166
Tru1I	TTAA	6 cut(s)	197, 273, 498, 1230, 1493, 1962
Tru9I	TTAA	6 cut(s)	197, 273, 498, 1230, 1493, 1962
TscAI	CASTG	3 cut(s)	411, 625, 832
TseFI	GTSAC	2 cut(s)	637, 2130
TseI	GCWGC	5 cut(s)	502, 1112, 1301, 1823, 2114
Tsp45I	GTSAC	2 cut(s)	637, 2130
TspRI	CASTG	3 cut(s)	411, 625, 832
Vha464I	CTTAAG	1 cut(s)	497
VneI	GTGCAC	4 cut(s)	1320, 1443, 1587, 1611
VpaK11BI	GGWCC	2 cut(s)	167, 877
XagI	CCTNNNNNAGG	1 cut(s)	317
XapI	RAATTY	4 cut(s)	908, 1158, 1714, 1844
XceI	RCATGY	5 cut(s)	85, 844, 975, 1055, 2048
XcmI	CCANNNNNNNNNTGG	1 cut(s)	351
XspI	CTAG	2 cut(s)	206, 2027
ZrmI	AGTACT	1 cut(s)	724
Zsp2I	ATGCAT	2 cut(s)	490, 1053

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.