RLG00000016399

FAD binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003028
Physical Location & Seq
Reverse (-)
6300039 .. 6301816
1778 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016399

Sequence Viewer

Length: 753 bp
ATGCCCAACCAACTATTGCTCATCTCAATCCAATGGCGACAATTCTGTGCTTCCGGTTCTGATAGTGGCTGCAGGACCTGTGGGTCTCGTTCTTTCTATACTCCTAACAAAGCTAGGGTCAAATGCGCAGTTTTGGAGAAAAGTACAAGTTTCTCCAGACATCCACAGGCACACTTCATTAACAATCGGTCGATGGAGATATTTCGCAAATTGGATGGCCTGGCAGAGGAGATCCAGAGGTCTCAACCACCAGTAGATTTGTGGAGAAAGTTTATATATTGCACTTCGCTTTCTGGTTCCATTCTTGGCTCAGTAGACCATATGCAACCTCCAGATTTTGAGCATGTTGTCAGCCCGGTCTCTGTTGCACACTTCTCTCAGTACAAACTGAATACATTACTAATTAAGCAACTTGAAAATCTCAGCTTCAAGTATTATACCCCTGACGGGTTGAAAGGCCTTGACCATGAACCCCTTGAGGCAAGGCAAATATTGATGGGCTATGAGTGTGTTTCCATCAATGCCAGTGATGATATTGTTTCCGTGACTGCATCTTTTCTCAAGGATGGGAAACATATGGAGAGGAATATCAGATGCAATATGGTTGTAGGTACTGACGGTGCTGGAAGTACTGTGCGTAAGCTGGCTGGTATAGATATGAGAGGTGAAAAGGACTTGCAGAAGCTTGTGAGTATTCAATTTGTTTTTGGTATTTACATTATGCATAATACACAAATAGTGAGATATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_3

Protein Analysis

251

Amino Acids

28.23

Weight (kDa)

8.8

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 40 - 235 2.1e-18 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 82
Acc16I TGCGCA 1 cut(s) 127
AccI GTMKAC 1 cut(s) 315
AclWI GGATC 1 cut(s) 226
AfaI GTAC 4 cut(s) 145, 383, 613, 631
AfiI CCNNNNNNNGG 2 cut(s) 226, 447
AgsI TTSAA 4 cut(s) 416, 430, 454, 698
AjnI CCWGG 1 cut(s) 219
AluBI AGCT 4 cut(s) 113, 426, 643, 685
AluI AGCT 4 cut(s) 113, 426, 643, 685
Alw26I GTCTC 3 cut(s) 90, 246, 364
AlwI GGATC 1 cut(s) 226
AlwNI CAGNNNCTG 1 cut(s) 78
AoxI GGCC 2 cut(s) 217, 457
ApeKI GCWGC 1 cut(s) 69
ArsI GACNNNNNNTTYG 2 cut(s) 102, 134
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 1 cut(s) 75
AsuC2I CCSGG 1 cut(s) 356
AsuHPI GGTGA 1 cut(s) 677
AvaII GGWCC 1 cut(s) 75
BbvI GCAGC 1 cut(s) 56
BccI CCATC 5 cut(s) 187, 209, 490, 524, 560
BciT130I CCWGG 1 cut(s) 221
BcnI CCSGG 1 cut(s) 356
BcoDI GTCTC 3 cut(s) 90, 246, 364
BfaI CTAG 1 cut(s) 114
BfmI CTRYAG 1 cut(s) 70
BisI GCNGC 1 cut(s) 70
BlsI GCNGC 1 cut(s) 71
BmcAI AGTACT 1 cut(s) 631
Bme1390I CCNGG 2 cut(s) 221, 356
Bme18I GGWCC 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 75
BmiI GGNNCC 1 cut(s) 298
BmrFI CCNGG 2 cut(s) 221, 356
BmsI GCATC 2 cut(s) 560, 584
BpmI CTGGAG 2 cut(s) 139, 315
BpuEI CTTGAG 2 cut(s) 497, 545
BpuMI CCSGG 1 cut(s) 356
BsaI GGTCTC 3 cut(s) 90, 246, 364
BsaWI WCCGGW 1 cut(s) 53
Bsc4I CCNNNNNNNGG 2 cut(s) 226, 447
Bse1I ACTGG 2 cut(s) 251, 525
BseBI CCWGG 1 cut(s) 221
BseGI GGATG 3 cut(s) 160, 220, 571
BseLI CCNNNNNNNGG 2 cut(s) 226, 447
BseMII CTCAG 3 cut(s) 324, 392, 436
BseNI ACTGG 2 cut(s) 251, 525
BseRI GAGGAG 1 cut(s) 242
BseXI GCAGC 1 cut(s) 56
Bsh1285I CGRYCG 1 cut(s) 191
BshFI GGCC 2 cut(s) 219, 459
BsiEI CGRYCG 1 cut(s) 191
BsiSI CCGG 2 cut(s) 54, 356
BslI CCNNNNNNNGG 2 cut(s) 226, 447
BsmAI GTCTC 3 cut(s) 90, 246, 364
BsnI GGCC 2 cut(s) 219, 459
Bso31I GGTCTC 3 cut(s) 90, 246, 364
Bsp143I GATC 1 cut(s) 231
BspANI GGCC 2 cut(s) 219, 459
BspCNI CTCAG 3 cut(s) 323, 391, 435
BspLI GGNNCC 1 cut(s) 298
BspMAI CTGCAG 1 cut(s) 74
BspPI GGATC 1 cut(s) 226
BspTNI GGTCTC 3 cut(s) 90, 246, 364
BsrI ACTGG 2 cut(s) 251, 525
BssMI GATC 1 cut(s) 231
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 2 cut(s) 620, 634
BstC8I GCNNGC 1 cut(s) 645
BstDEI CTNAG 3 cut(s) 310, 378, 422
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 3 cut(s) 160, 220, 571
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 1 cut(s) 234
BstMAI GTCTC 3 cut(s) 90, 246, 364
BstMBI GATC 1 cut(s) 231
BstMCI CGRYCG 1 cut(s) 191
BstNI CCWGG 1 cut(s) 221
BstNSI RCATGY 1 cut(s) 347
BstSCI CCNGG 2 cut(s) 219, 354
BstSFI CTRYAG 1 cut(s) 70
BstV1I GCAGC 1 cut(s) 56
BstX2I RGATCY 1 cut(s) 231
BstYI RGATCY 1 cut(s) 231
BsuRI GGCC 2 cut(s) 219, 459
BtsCI GGATG 3 cut(s) 160, 220, 571
BtsIMutI CAGTG 1 cut(s) 532
Cac8I GCNNGC 1 cut(s) 645
CaiI CAGNNNCTG 1 cut(s) 78
CfoI GCGC 1 cut(s) 128
Cfr13I GGNCC 1 cut(s) 75
Csp6I GTAC 4 cut(s) 144, 382, 612, 630
CviAII CATG 2 cut(s) 344, 467
CviQI GTAC 4 cut(s) 144, 382, 612, 630
DdeI CTNAG 3 cut(s) 310, 378, 422
DpnI GATC 1 cut(s) 233
DpnII GATC 1 cut(s) 231
DrdI GACNNNNNNGTC 1 cut(s) 82
DseDI GACNNNNNNGTC 1 cut(s) 82
Eco147I AGGCCT 1 cut(s) 459
Eco31I GGTCTC 3 cut(s) 90, 246, 364
Eco47I GGWCC 1 cut(s) 75
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoO109I RGGNCCY 1 cut(s) 75
EcoRII CCWGG 1 cut(s) 219
EcoT22I ATGCAT 1 cut(s) 726
FaeI CATG 2 cut(s) 347, 470
FatI CATG 2 cut(s) 343, 466
FauNDI CATATG 2 cut(s) 321, 576
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 1 cut(s) 70
FokI GGATG 3 cut(s) 147, 227, 578
Fsp4HI GCNGC 1 cut(s) 70
FspBI CTAG 1 cut(s) 114
FspI TGCGCA 1 cut(s) 127
GlaI GCGC 1 cut(s) 127
GluI GCNGC 1 cut(s) 70
GsuI CTGGAG 2 cut(s) 139, 315
HaeIII GGCC 2 cut(s) 219, 459
HapII CCGG 2 cut(s) 54, 356
HhaI GCGC 1 cut(s) 128
Hin1II CATG 2 cut(s) 347, 470
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HindIII AAGCTT 1 cut(s) 683
HpaII CCGG 2 cut(s) 54, 356
HphI GGTGA 1 cut(s) 677
Hpy166II GTNNAC 1 cut(s) 316
Hpy188I TCNGA 2 cut(s) 61, 593
Hpy188III TCNNGA 4 cut(s) 156, 235, 332, 750
Hpy8I GTNNAC 1 cut(s) 316
HpyCH4III ACNGT 2 cut(s) 620, 634
HpyCH4V TGCA 8 cut(s) 72, 282, 325, 368, 551, 597, 679, 724
HpyF3I CTNAG 3 cut(s) 310, 378, 422
Hsp92II CATG 2 cut(s) 347, 470
HspAI GCGC 1 cut(s) 126
Kzo9I GATC 1 cut(s) 231
Lsp1109I GCAGC 1 cut(s) 56
LweI GCATC 2 cut(s) 560, 584
MaeI CTAG 1 cut(s) 114
MaeIII GTNAC 1 cut(s) 544
MalI GATC 1 cut(s) 233
MboI GATC 1 cut(s) 231
MflI RGATCY 1 cut(s) 231
MluCI AATT 4 cut(s) 41, 209, 402, 698
MnlI CCTC 6 cut(s) 220, 231, 339, 472, 576, 656
Mph1103I ATGCAT 1 cut(s) 726
MseI TTAA 2 cut(s) 180, 405
MspI CCGG 2 cut(s) 54, 356
MspR9I CCNGG 2 cut(s) 221, 356
MvaI CCWGG 1 cut(s) 221
NciI CCSGG 1 cut(s) 356
NdeI CATATG 2 cut(s) 321, 576
NdeII GATC 1 cut(s) 231
NlaIII CATG 2 cut(s) 347, 470
NlaIV GGNNCC 1 cut(s) 298
NmuCI GTSAC 1 cut(s) 544
NsbI TGCGCA 1 cut(s) 127
NsiI ATGCAT 1 cut(s) 726
NspI RCATGY 1 cut(s) 347
PceI AGGCCT 1 cut(s) 459
PkrI GCNGC 1 cut(s) 71
PpuMI RGGWCCY 1 cut(s) 75
Psp5II RGGWCCY 1 cut(s) 75
Psp6I CCWGG 1 cut(s) 219
PspGI CCWGG 1 cut(s) 219
PspN4I GGNNCC 1 cut(s) 298
PspPI GGNCC 1 cut(s) 75
PspPPI RGGWCCY 1 cut(s) 75
PstI CTGCAG 1 cut(s) 74
PstNI CAGNNNCTG 1 cut(s) 78
PsuI RGATCY 1 cut(s) 231
RsaI GTAC 4 cut(s) 145, 383, 613, 631
RsaNI GTAC 4 cut(s) 144, 382, 612, 630
SaqAI TTAA 2 cut(s) 180, 405
SatI GCNGC 1 cut(s) 70
Sau3AI GATC 1 cut(s) 231
Sau96I GGNCC 1 cut(s) 75
ScaI AGTACT 1 cut(s) 631
ScrFI CCNGG 2 cut(s) 221, 356
SetI ASST 9 cut(s) 80, 115, 242, 331, 428, 613, 645, 667, 687
SfaNI GCATC 2 cut(s) 560, 584
SfcI CTRYAG 1 cut(s) 70
SinI GGWCC 1 cut(s) 75
SmlI CTYRAG 2 cut(s) 476, 560
SmoI CTYRAG 2 cut(s) 476, 560
Sse9I AATT 4 cut(s) 41, 209, 402, 698
SseBI AGGCCT 1 cut(s) 459
SspI AATATT 1 cut(s) 492
SspMI CTAG 1 cut(s) 114
StuI AGGCCT 1 cut(s) 459
StyD4I CCNGG 2 cut(s) 219, 354
TaaI ACNGT 2 cut(s) 620, 634
TaqI TCGA 1 cut(s) 191
TaqII GACCGA 1 cut(s) 177
TasI AATT 4 cut(s) 41, 209, 402, 698
TatI WGTACW 3 cut(s) 143, 381, 629
Tru1I TTAA 2 cut(s) 180, 405
Tru9I TTAA 2 cut(s) 180, 405
TscAI CASTG 1 cut(s) 532
TseFI GTSAC 1 cut(s) 544
TseI GCWGC 1 cut(s) 69
Tsp45I GTSAC 1 cut(s) 544
TspDTI ATGAA 2 cut(s) 166, 483
TspGWI ACGGA 1 cut(s) 532
TspRI CASTG 1 cut(s) 532
VpaK11BI GGWCC 1 cut(s) 75
XagI CCTNNNNNAGG 1 cut(s) 224
XceI RCATGY 1 cut(s) 347
XcmI CCANNNNNNNNNTGG 1 cut(s) 258
XmiI GTMKAC 1 cut(s) 315
XspI CTAG 1 cut(s) 114
ZrmI AGTACT 1 cut(s) 631
Zsp2I ATGCAT 1 cut(s) 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.