Rroxscaffold_2G00114870

FAD binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003028
Physical Location & Seq
Forward (+)
40962394 .. 40966531
4138 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00114870.1

Sequence Viewer

Length: 2175 bp
ATGGGTTGTATGGCGTCTTCACGGTTGTTCAAGAGGTTTAGCGGCCTATACAAACTCAATACCAGAATCCAACCATACCCATCTGCCTACGTCCCTACAAGAGCCCTATCACACTCTAATCTTTTCAGCAATGGCGACAATTCTGTGCTTCCGGTTCTGATAGTGGGTGCAGGACCTGTGGGTCTTGTTCTTTCTATACTCCTAACAAAGCTAGGGGTCAAATGTGCAGTTTTGGAGAAATGTACAAGTTTGTCCAGACATCCACAGGCACACTTCATTAACAATCGGTCGATGGAGATATTTCGCAAATTGGATGGCCTGGCAGAGGAGATCCAGAGGTCTCAACCACCAGTAGATTTGTGGAGAAAGTTTATATATTGCACTTCGCTTTCTGGTTCCATTCTTGGCTCAGTAGACCATATGCAACCTCAAGATTTTGAGCAAGTTGTCAGCCCGGTCTCTGTTGCACACTTCTCTCAGTACAAACTGAATACATTACTACTTAAGCAACTTGAAAATCTCAGCTTCAAGTATTATACCCCTGACAGGTTGAAAGGACTTGACCATGAACCCCTAGAGGCAAGGCAAATATTGATGGGCTATGAGTGTGTTTCCATCAATGCCAGTGATGATATTGTTTCGGTGACTGCATCTTTTCTCAAGGATGGGAAACATATGGAGACGAATATTAGATGCAATATGGTTGTAGGTACCGACGGTGCTGGAAGTACCGTACGTAAGCTGGCTGGTATAGATATGAGAGGTGAAAAGGACTTGCAGAAGCTTGTGAGTGTCCATTTCTTGAGCAGAGACCTTGGAAATTACTTGCACAGTGAGAGACCTGGCATGCTGTTTTTTATCTTCAATACTGAAGCTATAGGGGTCCTTGTTGCACATGATCTCAAGCAAGGGGAATTTGTATTGCAGATGCCATTTTATCCACCCCAGCAAAACCTTGAAGATTTCAGTCCCAAGACATGTGAGAGGATGATCTTCAAATTGGTAGGTCGAGAGCTTGGAGACATAAATGTGATTGACATAAAACCTTGGGTGATGCATGCTGAAGTTGCCGAGAAGTTTCTAAGTAGTCAAAACCGTGTAATTCTTGCTGGTGATGCTGCTCATCGATTCCCCCTGCTGGTGGATTTGGTTAGTGGTATGAATACCGGAATTCAGGATTCTCATAACCTTGCCTGGAAGATAGCTTCTGTAGTAAAGGGTATTGCACCACCTTCAATACTTAATACTTATGAAACAGAACGTAGGCCGATTGCCATTTTCAATACAACTCTTAGTATTCAGAACTTCAAAGCAGCCATGGCTGTTCCTGGTGCGCTTGGTCTTGATCCAACTATTGCAAACTCGGTACATCGAGTAATAAATAAGGGGGTTGGTTCCATTTTACCATCTGGACTACAGAGCGCCATTTTGGATGGAATTTTCATCTTAGGTCGTGCACAGTTATCAGAAAGTCTTTTGAATGAAAACAACCCAGTTGGAGCTTTAAGGCTTGCTAATCTAAGACGCATATTTGAAGAAGGAAAGAGTCTCCAGCTCCAGTTCCCGGCAGAAGATCTTGGTTTCAGGTACCTAGAAGGTGCACTTGTTCCTGACCATAATGGTGCACTGGGTGGTCCAGATGGACCTACCGGTCGCCGGAGGGACTATGTTCCTACTGCAGATCCAGGTTCAAGGCTTCCTCACATGAATGTGAGGATATTGTCAAATTCATCGAGTGAGGCAACTATTTCTACACTGGATCTTATATCTGGAGACAAAATTGAGTTTCTCCTTATTATAGCACCGATAGAGCCATCCTACCATCTTGCTCATGCTGCATTGAAGGTGGCTGAGGAATTCAAAGTTTCTGCAAGGGTGTGCGTGTTGTGGCCTGCTGGTAGTGCAAAAGGAGTGGAGGTAGGAAGTAAGGTATCATTGAAACCGCGGGACAATTACATGGACGTTATGGAAGTTAAAAAGTCATCAAATGCTTCGTCATGGTGGGACATCTGTCAAATGACTGATAAAGGAGCCATCCTAGTTCGGCCTGATGAGCATGTTGCTTGGCGTCTGAAGTCTGGAGTTGTTGGGGACCCTATGAGGGAAATGAGAAGGGCCTTTTCTGCTGCCCTAGGCTTCAAGTCACATATTAAAGACCAAGAAGCTGATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_3

Protein Analysis

724

Amino Acids

79.81

Weight (kDa)

8.23

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 51 - 425 2.1e-63 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 180, 1650
Acc65I GGTACC 2 cut(s) 710, 1587
AccB1I GGYRCC 2 cut(s) 710, 1587
AccI GTMKAC 1 cut(s) 414
AccII CGCG 1 cut(s) 1945
AciI CCGC 3 cut(s) 42, 1943, 1945
AclWI GGATC 4 cut(s) 325, 1340, 1676, 1767
AcsI RAATTY 5 cut(s) 914, 1170, 1437, 1726, 1856
AcuI CTGAAG 3 cut(s) 891, 1083, 2093
AcyI GRCGYC 2 cut(s) 14, 2068
AdeI CACNNNGTG 1 cut(s) 1631
AfaI GTAC 7 cut(s) 244, 482, 712, 730, 735, 1368, 1589
AfiI CCNNNNNNNGG 7 cut(s) 325, 546, 1138, 1141, 1564, 1656, 2101
AflII CTTAAG 1 cut(s) 503
AflIII ACRYGT 1 cut(s) 977
AgeI ACCGGT 1 cut(s) 1649
AjnI CCWGG 5 cut(s) 318, 841, 1193, 1327, 1684
Alw21I GWGCWC 3 cut(s) 1459, 1603, 1627
Alw26I GTCTC 8 cut(s) 345, 463, 674, 804, 832, 1014, 1553, 1767
Alw44I GTGCAC 3 cut(s) 1455, 1599, 1623
AlwI GGATC 4 cut(s) 325, 1340, 1676, 1767
AlwNI CAGNNNCTG 1 cut(s) 176
AoxI GGCC 6 cut(s) 43, 316, 1265, 1889, 2045, 2115
ApaLI GTGCAC 3 cut(s) 1455, 1599, 1623
ApeKI GCWGC 4 cut(s) 1118, 1313, 1835, 2126
ApoI RAATTY 5 cut(s) 914, 1170, 1437, 1726, 1856
ArsI GACNNNNNNTTYG 2 cut(s) 1979, 2011
AsiGI ACCGGT 1 cut(s) 1649
Asp718I GGTACC 2 cut(s) 710, 1587
AspA2I CCTAGG 1 cut(s) 2131
AspLEI GCGC 2 cut(s) 1336, 1424
AspS9I GGNCC 6 cut(s) 173, 883, 1634, 1643, 2092, 2115
AsuC2I CCSGG 2 cut(s) 455, 1565
AsuHPI GGTGA 4 cut(s) 655, 776, 1063, 1124
AvaII GGWCC 5 cut(s) 173, 883, 1634, 1643, 2092
AvrII CCTAGG 1 cut(s) 2131
BaeGI GKGCMC 3 cut(s) 1459, 1603, 1627
BanI GGYRCC 2 cut(s) 710, 1587
BanII GRGCYC 1 cut(s) 106
BarI GAAGNNNNNNTAC 1 cut(s) 33
BbsI GAAGAC 1 cut(s) 9
Bbv12I GWGCWC 3 cut(s) 1459, 1603, 1627
BbvCI CCTCAGC 1 cut(s) 1851
BbvI GCAGC 4 cut(s) 1105, 1325, 1822, 2113
BciT130I CCWGG 5 cut(s) 320, 843, 1195, 1329, 1686
BclI TGATCA 1 cut(s) 2167
BcnI CCSGG 2 cut(s) 455, 1565
BcoDI GTCTC 8 cut(s) 345, 463, 674, 804, 832, 1014, 1553, 1767
BfaI CTAG 5 cut(s) 212, 575, 1592, 2039, 2132
BfmI CTRYAG 4 cut(s) 876, 1209, 1415, 1677
BfoI RGCGCY 1 cut(s) 1425
BfrI CTTAAG 1 cut(s) 503
BglII AGATCT 1 cut(s) 1573
BisI GCNGC 5 cut(s) 43, 1119, 1314, 1836, 2127
BlnI CCTAGG 1 cut(s) 2131
BlsI GCNGC 5 cut(s) 44, 1120, 1315, 1837, 2128
Bme1390I CCNGG 7 cut(s) 320, 455, 843, 1195, 1329, 1565, 1686
Bme18I GGWCC 5 cut(s) 173, 883, 1634, 1643, 2092
BmgT120I GGNCC 6 cut(s) 173, 883, 1634, 1643, 2092, 2115
BmiI GGNNCC 8 cut(s) 397, 712, 884, 1396, 1589, 2032, 2093, 2094
BmrFI CCNGG 7 cut(s) 320, 455, 843, 1195, 1329, 1565, 1686
BmrI ACTGGG 2 cut(s) 1487, 1637
BmsI GCATC 5 cut(s) 659, 683, 918, 1044, 1105
BmuI ACTGGG 2 cut(s) 1487, 1637
BoxI GACNNNNGTC 1 cut(s) 2010
BpiI GAAGAC 1 cut(s) 9
BpmI CTGGAG 4 cut(s) 1535, 1541, 1791, 2100
Bpu10I CCTNAGC 1 cut(s) 1851
BpuEI CTTGAG 4 cut(s) 414, 644, 823, 887
BpuMI CCSGG 2 cut(s) 455, 1565
Bsa29I ATCGAT 1 cut(s) 1126
BsaAI YACGTR 1 cut(s) 737
BsaHI GRCGYC 2 cut(s) 14, 2068
BsaI GGTCTC 4 cut(s) 345, 463, 804, 832
BsaJI CCNNGG 5 cut(s) 814, 1046, 1317, 1943, 2131
BsaWI WCCGGW 3 cut(s) 151, 1166, 1649
BsaXI ACNNNNNCTCC 2 cut(s) 1902, 1932
Bsc4I CCNNNNNNNGG 7 cut(s) 325, 546, 1138, 1141, 1564, 1656, 2101
Bse118I RCCGGY 1 cut(s) 1649
Bse1I ACTGG 6 cut(s) 350, 624, 1493, 1558, 1632, 1761
Bse3DI GCAATG 1 cut(s) 136
BseBI CCWGG 5 cut(s) 320, 843, 1195, 1329, 1686
BseCI ATCGAT 1 cut(s) 1126
BseDI CCNNGG 5 cut(s) 814, 1046, 1317, 1943, 2131
BseGI GGATG 7 cut(s) 259, 319, 670, 993, 1438, 1814, 2034
BseLI CCNNNNNNNGG 7 cut(s) 325, 546, 1138, 1141, 1564, 1656, 2101
BseMI GCAATG 1 cut(s) 136
BseMII CTCAG 4 cut(s) 423, 491, 535, 1842
BseNI ACTGG 6 cut(s) 350, 624, 1493, 1558, 1632, 1761
BseRI GAGGAG 1 cut(s) 341
BseSI GKGCMC 3 cut(s) 1459, 1603, 1627
BseXI GCAGC 4 cut(s) 1105, 1325, 1822, 2113
BseYI CCCAGC 1 cut(s) 945
BsgI GTGCAG 2 cut(s) 189, 246
Bsh1236I CGCG 1 cut(s) 1945
Bsh1285I CGRYCG 2 cut(s) 290, 1654
BshFI GGCC 6 cut(s) 45, 318, 1267, 1891, 2047, 2117
BshNI GGYRCC 2 cut(s) 710, 1587
BshTI ACCGGT 1 cut(s) 1649
BshVI ATCGAT 1 cut(s) 1126
BsiEI CGRYCG 2 cut(s) 290, 1654
BsiHKAI GWGCWC 3 cut(s) 1459, 1603, 1627
BsiSI CCGG 6 cut(s) 152, 455, 1167, 1565, 1650, 1657
BsiWI CGTACG 1 cut(s) 733
BslFI GGGAC 6 cut(s) 77, 954, 1676, 1961, 2018, 2105
BslI CCNNNNNNNGG 7 cut(s) 325, 546, 1138, 1141, 1564, 1656, 2101
BsmAI GTCTC 8 cut(s) 345, 463, 674, 804, 832, 1014, 1553, 1767
BsmBI CGTCTC 1 cut(s) 674
BsmFI GGGAC 6 cut(s) 77, 954, 1676, 1961, 2018, 2105
BsnI GGCC 6 cut(s) 45, 318, 1267, 1891, 2047, 2117
Bso31I GGTCTC 4 cut(s) 345, 463, 804, 832
Bsp1286I GDGCHC 4 cut(s) 106, 1459, 1603, 1627
Bsp1407I TGTACA 1 cut(s) 242
Bsp143I GATC 8 cut(s) 330, 898, 990, 1345, 1573, 1681, 1759, 2167
Bsp19I CCATGG 1 cut(s) 1317
BspACI CCGC 3 cut(s) 42, 1943, 1945
BspANI GGCC 6 cut(s) 45, 318, 1267, 1891, 2047, 2117
BspCNI CTCAG 4 cut(s) 422, 490, 534, 1843
BspDI ATCGAT 1 cut(s) 1126
BspFNI CGCG 1 cut(s) 1945
BspLI GGNNCC 8 cut(s) 397, 712, 884, 1396, 1589, 2032, 2093, 2094
BspMAI CTGCAG 1 cut(s) 1681
BspPI GGATC 4 cut(s) 325, 1340, 1676, 1767
BspT107I GGYRCC 2 cut(s) 710, 1587
BspTI CTTAAG 1 cut(s) 503
BspTNI GGTCTC 4 cut(s) 345, 463, 804, 832
BsrDI GCAATG 1 cut(s) 136
BsrFI RCCGGY 1 cut(s) 1649
BsrGI TGTACA 1 cut(s) 242
BsrI ACTGG 6 cut(s) 350, 624, 1493, 1558, 1632, 1761
BssAI RCCGGY 1 cut(s) 1649
BssECI CCNNGG 5 cut(s) 814, 1046, 1317, 1943, 2131
BssMI GATC 8 cut(s) 330, 898, 990, 1345, 1573, 1681, 1759, 2167
BssNI GRCGYC 2 cut(s) 14, 2068
BssT1I CCWWGG 4 cut(s) 814, 1046, 1317, 2131
Bst2UI CCWGG 5 cut(s) 320, 843, 1195, 1329, 1686
Bst4CI ACNGT 6 cut(s) 24, 719, 733, 833, 1097, 1461
BstACI GRCGYC 2 cut(s) 14, 2068
BstAFI CTTAAG 1 cut(s) 503
BstAUI TGTACA 1 cut(s) 242
BstBAI YACGTR 1 cut(s) 737
BstC8I GCNNGC 5 cut(s) 744, 848, 1059, 1512, 1893
BstDEI CTNAG 8 cut(s) 409, 477, 521, 1082, 1292, 1447, 1520, 1851
BstDSI CCRYGG 2 cut(s) 1317, 1943
BstENI CCTNNNNNAGG 1 cut(s) 323
BstF5I GGATG 7 cut(s) 259, 319, 670, 993, 1438, 1814, 2034
BstFNI CGCG 1 cut(s) 1945
BstH2I RGCGCY 1 cut(s) 1425
BstHHI GCGC 2 cut(s) 1336, 1424
BstKTI GATC 8 cut(s) 333, 901, 993, 1348, 1576, 1684, 1762, 2170
BstMAI GTCTC 8 cut(s) 345, 463, 674, 804, 832, 1014, 1553, 1767
BstMBI GATC 8 cut(s) 330, 898, 990, 1345, 1573, 1681, 1759, 2167
BstMCI CGRYCG 2 cut(s) 290, 1654
BstMWI GCNNNNNNNGC 7 cut(s) 1067, 1115, 1319, 1835, 1901, 2053, 2123
BstNI CCWGG 5 cut(s) 320, 843, 1195, 1329, 1686
BstNSI RCATGY 4 cut(s) 850, 981, 1061, 2060
BstPAI GACNNNNGTC 1 cut(s) 2010
BstSCI CCNGG 7 cut(s) 318, 453, 841, 1193, 1327, 1563, 1684
BstSFI CTRYAG 4 cut(s) 876, 1209, 1415, 1677
BstSLI GKGCMC 3 cut(s) 1459, 1603, 1627
BstSNI TACGTA 1 cut(s) 737
BstUI CGCG 1 cut(s) 1945
BstV1I GCAGC 4 cut(s) 1105, 1325, 1822, 2113
BstV2I GAAGAC 1 cut(s) 9
BstX2I RGATCY 4 cut(s) 330, 1573, 1681, 1759
BstYI RGATCY 4 cut(s) 330, 1573, 1681, 1759
Bsu15I ATCGAT 1 cut(s) 1126
BsuRI GGCC 6 cut(s) 45, 318, 1267, 1891, 2047, 2117
BsuTUI ATCGAT 1 cut(s) 1126
BtgI CCRYGG 2 cut(s) 1317, 1943
BtsCI GGATG 7 cut(s) 259, 319, 670, 993, 1438, 1814, 2034
BtsIMutI CAGTG 4 cut(s) 631, 838, 1625, 1754
Cac8I GCNNGC 5 cut(s) 744, 848, 1059, 1512, 1893
CaiI CAGNNNCTG 1 cut(s) 176
CfoI GCGC 2 cut(s) 1336, 1424
Cfr10I RCCGGY 1 cut(s) 1649
Cfr13I GGNCC 6 cut(s) 173, 883, 1634, 1643, 2092, 2115
Cfr42I CCGCGG 1 cut(s) 1946
ClaI ATCGAT 1 cut(s) 1126
CseI GACGC 3 cut(s) 3, 1533, 2057
Csp6I GTAC 7 cut(s) 243, 481, 711, 729, 734, 1367, 1588
CspAI ACCGGT 1 cut(s) 1649
CspCI CAANNNNNGTGG 2 cut(s) 1893, 1928
CviQI GTAC 7 cut(s) 243, 481, 711, 729, 734, 1367, 1588
DdeI CTNAG 8 cut(s) 409, 477, 521, 1082, 1292, 1447, 1520, 1851
DpnI GATC 8 cut(s) 332, 900, 992, 1347, 1575, 1683, 1761, 2169
DpnII GATC 8 cut(s) 330, 898, 990, 1345, 1573, 1681, 1759, 2167
DraIII CACNNNGTG 1 cut(s) 1631
DrdI GACNNNNNNGTC 2 cut(s) 180, 1650
DseDI GACNNNNNNGTC 2 cut(s) 180, 1650
Eco105I TACGTA 1 cut(s) 737
Eco130I CCWWGG 4 cut(s) 814, 1046, 1317, 2131
Eco24I GRGCYC 1 cut(s) 106
Eco31I GGTCTC 4 cut(s) 345, 463, 804, 832
Eco47I GGWCC 5 cut(s) 173, 883, 1634, 1643, 2092
Eco57I CTGAAG 3 cut(s) 891, 1083, 2093
EcoNI CCTNNNNNAGG 1 cut(s) 323
EcoO109I RGGNCCY 4 cut(s) 173, 883, 2092, 2115
EcoRI GAATTC 2 cut(s) 1170, 1856
EcoRII CCWGG 5 cut(s) 318, 841, 1193, 1327, 1684
EcoT14I CCWWGG 4 cut(s) 814, 1046, 1317, 2131
EcoT22I ATGCAT 1 cut(s) 1059
EcoT38I GRGCYC 1 cut(s) 106
ErhI CCWWGG 4 cut(s) 814, 1046, 1317, 2131
Esp3I CGTCTC 1 cut(s) 674
FalI AAGNNNNNCTT 2 cut(s) 1587, 1619
FaqI GGGAC 6 cut(s) 77, 954, 1676, 1961, 2018, 2105
FauI CCCGC 1 cut(s) 1938
FauNDI CATATG 2 cut(s) 420, 675
FbaI TGATCA 1 cut(s) 2167
FblI GTMKAC 1 cut(s) 414
Fnu4HI GCNGC 5 cut(s) 43, 1119, 1314, 1836, 2127
FokI GGATG 7 cut(s) 246, 326, 677, 1000, 1445, 1801, 2021
FriOI GRGCYC 1 cut(s) 106
Fsp4HI GCNGC 5 cut(s) 43, 1119, 1314, 1836, 2127
FspBI CTAG 5 cut(s) 212, 575, 1592, 2039, 2132
GlaI GCGC 2 cut(s) 1335, 1423
GluI GCNGC 5 cut(s) 43, 1119, 1314, 1836, 2127
GsaI CCCAGC 1 cut(s) 949
GsuI CTGGAG 4 cut(s) 1535, 1541, 1791, 2100
HaeII RGCGCY 1 cut(s) 1425
HaeIII GGCC 6 cut(s) 45, 318, 1267, 1891, 2047, 2117
HapII CCGG 6 cut(s) 152, 455, 1167, 1565, 1650, 1657
HgaI GACGC 3 cut(s) 3, 1533, 2057
HhaI GCGC 2 cut(s) 1336, 1424
Hin1I GRCGYC 2 cut(s) 14, 2068
Hin6I GCGC 2 cut(s) 1334, 1422
HinP1I GCGC 2 cut(s) 1334, 1422
HindIII AAGCTT 1 cut(s) 782
HinfI GANTC 4 cut(s) 66, 1128, 1178, 1546
HpaII CCGG 6 cut(s) 152, 455, 1167, 1565, 1650, 1657
HphI GGTGA 4 cut(s) 655, 776, 1063, 1124
Hpy166II GTNNAC 4 cut(s) 415, 1457, 1601, 1625
Hpy188I TCNGA 4 cut(s) 159, 1302, 1468, 2073
Hpy8I GTNNAC 4 cut(s) 415, 1457, 1601, 1625
Hpy99I CGWCG 1 cut(s) 719
HpyAV CCTTC 5 cut(s) 1242, 1532, 1589, 1837, 2106
HpyCH4III ACNGT 6 cut(s) 24, 719, 733, 833, 1097, 1461
HpyCH4IV ACGT 4 cut(s) 90, 736, 1261, 1962
HpyF10VI GCNNNNNNNGC 7 cut(s) 1067, 1115, 1319, 1835, 1901, 2053, 2123
HpyF3I CTNAG 8 cut(s) 409, 477, 521, 1082, 1292, 1447, 1520, 1851
HpySE526I ACGT 4 cut(s) 90, 736, 1261, 1962
Hsp92I GRCGYC 2 cut(s) 14, 2068
HspAI GCGC 2 cut(s) 1334, 1422
KflI GGGWCCC 1 cut(s) 2092
KpnI GGTACC 2 cut(s) 714, 1591
Ksp22I TGATCA 1 cut(s) 2167
KspI CCGCGG 1 cut(s) 1946
Kzo9I GATC 8 cut(s) 330, 898, 990, 1345, 1573, 1681, 1759, 2167
LmnI GCTCC 3 cut(s) 1499, 1560, 2030
Lsp1109I GCAGC 4 cut(s) 1105, 1325, 1822, 2113
LweI GCATC 5 cut(s) 659, 683, 918, 1044, 1105
MaeI CTAG 5 cut(s) 212, 575, 1592, 2039, 2132
MaeII ACGT 4 cut(s) 90, 736, 1261, 1962
MaeIII GTNAC 2 cut(s) 643, 2142
MalI GATC 8 cut(s) 332, 900, 992, 1347, 1575, 1683, 1761, 2169
MboI GATC 8 cut(s) 330, 898, 990, 1345, 1573, 1681, 1759, 2167
MboII GAAGA 7 cut(s) 9, 853, 971, 985, 1210, 1547, 1583
MflI RGATCY 4 cut(s) 330, 1573, 1681, 1759
MhlI GDGCHC 4 cut(s) 106, 1459, 1603, 1627
MlyI GAGTC 1 cut(s) 1555
MmeI TCCRAC 3 cut(s) 94, 1373, 1477
Mph1103I ATGCAT 1 cut(s) 1059
MseI TTAA 6 cut(s) 279, 504, 1242, 1505, 1974, 2151
MslI CAYNNNNRTG 4 cut(s) 1028, 1620, 1707, 1709
MspA1I CMGCKG 1 cut(s) 1945
MspCI CTTAAG 1 cut(s) 503
MspI CCGG 6 cut(s) 152, 455, 1167, 1565, 1650, 1657
MspR9I CCNGG 7 cut(s) 320, 455, 843, 1195, 1329, 1565, 1686
MvaI CCWGG 5 cut(s) 320, 843, 1195, 1329, 1686
MvnI CGCG 1 cut(s) 1945
MwoI GCNNNNNNNGC 7 cut(s) 1067, 1115, 1319, 1835, 1901, 2053, 2123
NciI CCSGG 2 cut(s) 455, 1565
NcoI CCATGG 1 cut(s) 1317
NdeI CATATG 2 cut(s) 420, 675
NdeII GATC 8 cut(s) 330, 898, 990, 1345, 1573, 1681, 1759, 2167
NlaIV GGNNCC 8 cut(s) 397, 712, 884, 1396, 1589, 2032, 2093, 2094
NmeAIII GCCGAG 1 cut(s) 1096
NmuCI GTSAC 2 cut(s) 643, 2142
NsiI ATGCAT 1 cut(s) 1059
NspI RCATGY 4 cut(s) 850, 981, 1061, 2060
PaeI GCATGC 2 cut(s) 850, 1061
PciI ACATGT 1 cut(s) 977
PfeI GAWTC 3 cut(s) 66, 1128, 1178
Pfl23II CGTACG 1 cut(s) 733
PinAI ACCGGT 1 cut(s) 1649
PkrI GCNGC 5 cut(s) 44, 1120, 1315, 1837, 2128
PleI GAGTC 1 cut(s) 1554
PpsI GAGTC 1 cut(s) 1554
Ppu21I YACGTR 1 cut(s) 737
PpuMI RGGWCCY 3 cut(s) 173, 883, 2092
PscI ACATGT 1 cut(s) 977
PshAI GACNNNNGTC 1 cut(s) 2010
Psp5II RGGWCCY 3 cut(s) 173, 883, 2092
Psp6I CCWGG 5 cut(s) 318, 841, 1193, 1327, 1684
PspFI CCCAGC 1 cut(s) 945
PspGI CCWGG 5 cut(s) 318, 841, 1193, 1327, 1684
PspLI CGTACG 1 cut(s) 733
PspN4I GGNNCC 8 cut(s) 397, 712, 884, 1396, 1589, 2032, 2093, 2094
PspPI GGNCC 6 cut(s) 173, 883, 1634, 1643, 2092, 2115
PspPPI RGGWCCY 3 cut(s) 173, 883, 2092
PstI CTGCAG 1 cut(s) 1681
PstNI CAGNNNCTG 1 cut(s) 176
PsuI RGATCY 4 cut(s) 330, 1573, 1681, 1759
RsaI GTAC 7 cut(s) 244, 482, 712, 730, 735, 1368, 1589
RsaNI GTAC 7 cut(s) 243, 481, 711, 729, 734, 1367, 1588
RseI CAYNNNNRTG 4 cut(s) 1028, 1620, 1707, 1709
SacII CCGCGG 1 cut(s) 1946
SaqAI TTAA 6 cut(s) 279, 504, 1242, 1505, 1974, 2151
SatI GCNGC 5 cut(s) 43, 1119, 1314, 1836, 2127
Sau3AI GATC 8 cut(s) 330, 898, 990, 1345, 1573, 1681, 1759, 2167
Sau96I GGNCC 6 cut(s) 173, 883, 1634, 1643, 2092, 2115
SchI GAGTC 1 cut(s) 1555
ScrFI CCNGG 7 cut(s) 320, 455, 843, 1195, 1329, 1565, 1686
SduI GDGCHC 4 cut(s) 106, 1459, 1603, 1627
SfaNI GCATC 5 cut(s) 659, 683, 918, 1044, 1105
SfcI CTRYAG 4 cut(s) 876, 1209, 1415, 1677
Sfr303I CCGCGG 1 cut(s) 1946
SgrBI CCGCGG 1 cut(s) 1946
SinI GGWCC 5 cut(s) 173, 883, 1634, 1643, 2092
SmiMI CAYNNNNRTG 4 cut(s) 1028, 1620, 1707, 1709
SmlI CTYRAG 5 cut(s) 429, 503, 659, 802, 902
SmoI CTYRAG 5 cut(s) 429, 503, 659, 802, 902
SnaBI TACGTA 1 cut(s) 737
SphI GCATGC 2 cut(s) 850, 1061
SsiI CCGC 3 cut(s) 42, 1943, 1945
SspI AATATT 2 cut(s) 591, 688
SspMI CTAG 5 cut(s) 212, 575, 1592, 2039, 2132
StyD4I CCNGG 7 cut(s) 318, 453, 841, 1193, 1327, 1563, 1684
StyI CCWWGG 4 cut(s) 814, 1046, 1317, 2131
TaaI ACNGT 6 cut(s) 24, 719, 733, 833, 1097, 1461
TaiI ACGT 4 cut(s) 93, 739, 1264, 1965
TaqI TCGA 5 cut(s) 290, 1009, 1126, 1372, 1733
TaqII GACCGA 1 cut(s) 276
TatI WGTACW 2 cut(s) 242, 480
TauI GCSGC 1 cut(s) 45
TfiI GAWTC 3 cut(s) 66, 1128, 1178
Tru1I TTAA 6 cut(s) 279, 504, 1242, 1505, 1974, 2151
Tru9I TTAA 6 cut(s) 279, 504, 1242, 1505, 1974, 2151
TscAI CASTG 4 cut(s) 631, 838, 1632, 1761
TseFI GTSAC 2 cut(s) 643, 2142
TseI GCWGC 4 cut(s) 1118, 1313, 1835, 2126
Tsp45I GTSAC 2 cut(s) 643, 2142
TspDTI ATGAA 8 cut(s) 265, 582, 1175, 1266, 1432, 1497, 1719, 1721
TspRI CASTG 4 cut(s) 631, 838, 1632, 1761
Vha464I CTTAAG 1 cut(s) 503
VneI GTGCAC 3 cut(s) 1455, 1599, 1623
VpaK11BI GGWCC 5 cut(s) 173, 883, 1634, 1643, 2092
XagI CCTNNNNNAGG 1 cut(s) 323
XapI RAATTY 5 cut(s) 914, 1170, 1437, 1726, 1856
XceI RCATGY 4 cut(s) 850, 981, 1061, 2060
XcmI CCANNNNNNNNNTGG 1 cut(s) 357
XmaJI CCTAGG 1 cut(s) 2131
XmiI GTMKAC 1 cut(s) 414
XspI CTAG 5 cut(s) 212, 575, 1592, 2039, 2132
Zsp2I ATGCAT 1 cut(s) 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.