Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003028

Physical Location & Seq

Genomic Coordinates Reverse (-)

8224599 .. 8229057

Gene Structure 4459 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1113200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2157 bp

ATGGCAGTTTCGCGGTTGTTCAGGAGTTTTAGCGGCCTACACAAAGCCAATGCCAGAATACAAGCATACCCATTTGGCTACAAGCATAGCAGAGCCTTATCATCGGACTCTTTCAACAATGGAGACGATACGGTGCTTCCGGTTCTGATTGTCGGTGCAGGACCTGTGGGTCTCGTTTTGTCCATACTTTTAACAAAGCTAGGGGTCAAATGTTCGGTTGTGGAGAAGAGCAAAACTTTCTCGAATCATCCACAGGCGCACTTCATCAACAATCGATCCATGGAGGTGTTTCGCAAATTGGATGGCCTGGCGGAGGAGATCCAGAGGTCTCAACCACCAGTAGATTTATGGAGGAAGTTCATATATTGCACTTCGCTCTATGGTTCAATTCTTGGGTCGGTTGACCATATGCAACCCCAAGATTTTGAGCAAGTCGTCAGCCCGGTCTCTGTTGCACACTTCTCACAGTACAAACTGATTAGTTTACTACTTAAGCAGCTTGAAAATCTCAGCTTCAAGTTCTGTACATCTGATGGGTTGGAAGGCCTCAACCATGGAACCTTTCAGGAAAAGCAACTATTAATGGGGCATGAGTGTGTTTCCATCAAAGCGAGTGATGATTTTGTTTCTGTGACTGCATCTTTTCTCAAGGATGGGAAGCGTATGGAAAGGAATATCAGATGTAATATCCTTGTTGGTACAGATGGAGCTGGAAGTACTGTACGTAAGCTGGCTGGAGTGGATATGAGAGGTGAAAAGGACTTACAGAAGCTTGTGAGTGTCCATTTCTTGAGCAAAGGTCTTGGGAAGTATTTACTCTGTGAGAGACCTGGCATGCTTTTTTTCATCTTCAATACTGAAGCTATAGGGGTCCTTGTTGCTCATGATCTCGAGCAAGGGGAATTTGTATTACAGATTCCGTTTTATCCACCTCAGCAAAACCTTGAAGATTTCAGTCGCCAGATATGTGAGCAAATAATCTTCAAGTTGGTTGGTCGAGAGCTAGGAGACATAAATGTGATTGACATAAAACCATGGGTTATGCATGCTGAAGTTGCGGAGAAGTTTCTAAGTTGTGGCAACCGAATAATACTTGCTGGCGATGCTGCTCATCGATTCCCTCCTGCCGGAGGGTTTGGAATGAATACTGGAATTCAGGACTCTCATAATCTTGCATGGAAAATAGCTTCGGTAGTAGAGGGTACTGCACCGACTTCAATACTTAATTCTTATGAAACAGAACGTAGGCCGATTGCTGTTTTCAATACAGAACTTAGTATTGAGAACTTCAAAGCAGCCATGGCTGTTCCTGCTGCACTTGGTCTTGATCCAACTGTTGCAAACTCAGTGCATCGCGTCATAAATGAGGGGGTTGGTTCCATTTTACCATCTGAATTACAGAGGGCCATTTTGGATGGAATTTTCACAATAGGTCGTGCCCAGCTATCGGAATCTTTTTTGAATGAAAACAACCCACTTGGGTCGTCAAGGCTTGCTAAGCTAAGGCGCATATTTGAAGAAGGAAAGAGCCTCCAACTCCAGTTCCCAGCAGAGGATCTTGGTTTTAGGTATCTAGAAGGTGCACTTATCCCTGATAGTGATGGTGGAATGAGTGCTCCAGAAGGACCTACTGGTCGCCGGAGGGACTATGTTCCTTCTGTGGTTCCAGGTGCAAGGCTCCCCCACATGAACGTGAGGATATTGTCAGATACCTCAAGTGAGGTTACTATTTCTACACTGGACCTTATATCTGGAGACAAGGTCGAGTTTCTCCTCATTATATCGCCAACCGAGCCGTCTTACCATCTTGCTCATGCTGCATTCAAAGTGGCCGAGGAATTCAAAGTTTCTGCAAGGGTGTGCGTGTTGTGGCCTGCCGGCAGTGTTAAGGGAGTCGAAATAAAGAGTAAGGCATCATTGGCACCCTGGGAGAATTACATTGACCTCGTGGAAGTTAAAAAGTCACCAAATTCGTCATCATGGTGGGATATCTGCCAAATGAATGACAAAGGAGCCATCTTAGTTCGACCGGATGAGCATGTTGCTTGGCGTGTGAAGTCAGGGGTTGTCCGTGACCCTATAACGGAAATGAGAAGGGTATTCTCGGCTACTCTCGGCATAAAACAACATACTAAAGATCAAGAAACTGACACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_3

Protein Analysis

719

Amino Acids

79.27

Weight (kDa)

6.57

Isoelectric Point (pI)

40.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_3	PF01494	47 - 419	2.4e-68	FAD binding domain
Rng_hyd_C	PF21274	637 - 701	4.3e-07	Aromatic-ring hydroxylase, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003028)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24340
fragaria_vesca	FvH4_4g24220 FvH4_4g24220 FvH4_4g24220 FvH4_4g24220 FvH4_4g24220 FvH4_4g24220 FvH4_4g24220 FvH4_4g24220 FvH4_4g24220 FvH4_4g24220
malus_domestica	MD13G1113200.v1.1 MD16G1112300.v1.1
prunus_persica	Prupe.1G232900_v2.0.a1
pyrus_communis	pycom13g09860
rosa_chinensis	RchiOBHm_Chr4g0431061
rosa_laevigata	RLG00000006928 RLG00000016399
rosa_multiflora	Rmu_co8265599.1_g000001 Rmu_sc0003592.1_g000008 Rmu_sc0010203.1_g000008 Rmu_sc0041255.1_g000001 Rmu_sc0043112.1_g000001
rosa_roxburghii	Rroxscaffold_2G00114870 Rroxscaffold_2G00148110 Rroxscaffold_5G00372680
rosa_rugosa	Rorug02G0035800 Rorug02G0035800 Rorug02G0035900 Rorug02G0035900 Rorug04G0247900
rosa_samantha	Rh2DG080900 Rh2DG081100 Rh4AG304600 Rh4BG311800 Rh4CG328100 Rh4DG308300
rosa_wichuraiana	Rw4G026450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	168, 1632
AccB1I	GGYRCC	1 cut(s)	1921
AccII	CGCG	2 cut(s)	13, 1356
AciI	CCGC	4 cut(s)	13, 33, 311, 1058
AclWI	GGATC	4 cut(s)	270, 313, 1322, 1563
AcoI	YGGCCR	1 cut(s)	1830
AcsI	RAATTY	5 cut(s)	902, 1152, 1419, 1838, 1969
AcuI	CTGAAG	2 cut(s)	879, 1071
AfaI	GTAC	6 cut(s)	470, 526, 700, 718, 723, 1204
AfiI	CCNNNNNNNGG	6 cut(s)	313, 1127, 1130, 1447, 1552, 2083
AflII	CTTAAG	1 cut(s)	491
AjnI	CCWGG	4 cut(s)	306, 829, 1666, 1925
Alw21I	GWGCWC	2 cut(s)	1585, 1618
Alw26I	GTCTC	7 cut(s)	117, 176, 333, 451, 820, 1002, 1749
Alw44I	GTGCAC	1 cut(s)	1581
AlwI	GGATC	4 cut(s)	270, 313, 1322, 1563
AlwNI	CAGNNNCTG	1 cut(s)	164
Ama87I	CYCGRG	1 cut(s)	890
AoxI	GGCC	7 cut(s)	34, 304, 544, 1247, 1404, 1830, 1871
ApaLI	GTGCAC	1 cut(s)	1581
ApeKI	GCWGC	5 cut(s)	496, 1106, 1295, 1313, 1817
ApoI	RAATTY	5 cut(s)	902, 1152, 1419, 1838, 1969
AseI	ATTAAT	1 cut(s)	581
AspLEI	GCGC	2 cut(s)	259, 1509
AspS9I	GGNCC	5 cut(s)	161, 871, 1404, 1625, 1741
AsuC2I	CCSGG	1 cut(s)	443
AsuHPI	GGTGA	2 cut(s)	764, 1956
AvaI	CYCGRG	1 cut(s)	890
AvaII	GGWCC	4 cut(s)	161, 871, 1625, 1741
BaeGI	GKGCMC	2 cut(s)	1441, 1585
BanI	GGYRCC	1 cut(s)	1921
BauI	CACGAG	1 cut(s)	1946
Bbv12I	GWGCWC	2 cut(s)	1585, 1618
BbvCI	CCTCAGC	1 cut(s)	933
BbvI	GCAGC	5 cut(s)	508, 1093, 1300, 1307, 1804
BceAI	ACGGC	1 cut(s)	1780
BciT130I	CCWGG	4 cut(s)	308, 831, 1668, 1927
BcnI	CCSGG	1 cut(s)	443
BcoDI	GTCTC	7 cut(s)	117, 176, 333, 451, 820, 1002, 1749
BfaI	CTAG	3 cut(s)	200, 1004, 1574
BfmI	CTRYAG	1 cut(s)	864
BfrI	CTTAAG	1 cut(s)	491
BisI	GCNGC	6 cut(s)	34, 497, 1107, 1296, 1314, 1818
BlpI	GCTNAGC	1 cut(s)	1497
BlsI	GCNGC	6 cut(s)	35, 498, 1108, 1297, 1315, 1819
BmcAI	AGTACT	1 cut(s)	718
Bme1390I	CCNGG	5 cut(s)	308, 443, 831, 1668, 1927
Bme18I	GGWCC	4 cut(s)	161, 871, 1625, 1741
BmeT110I	CYCGRG	1 cut(s)	890
BmgT120I	GGNCC	5 cut(s)	161, 871, 1404, 1625, 1741
BmiI	GGNNCC	7 cut(s)	559, 872, 1378, 1665, 1679, 1923, 2014
BmrFI	CCNGG	5 cut(s)	308, 443, 831, 1668, 1927
BmsI	GCATC	4 cut(s)	647, 1093, 1360, 1922
BpmI	CTGGAG	4 cut(s)	756, 1523, 1602, 1773
Bpu10I	CCTNAGC	2 cut(s)	933, 1502
Bpu1102I	GCTNAGC	1 cut(s)	1497
BpuEI	CTTGAG	3 cut(s)	632, 811, 1699
BpuMI	CCSGG	1 cut(s)	443
Bsa29I	ATCGAT	2 cut(s)	274, 1114
BsaAI	YACGTR	1 cut(s)	725
BsaI	GGTCTC	4 cut(s)	176, 333, 451, 820
BsaJI	CCNNGG	7 cut(s)	279, 553, 1034, 1299, 1833, 1925, 1926
BsaWI	WCCGGW	2 cut(s)	139, 2029
BsaXI	ACNNNNNCTCC	2 cut(s)	1746, 1776
Bsc4I	CCNNNNNNNGG	6 cut(s)	313, 1127, 1130, 1447, 1552, 2083
Bse118I	RCCGGY	1 cut(s)	1877
Bse1I	ACTGG	5 cut(s)	338, 1153, 1540, 1636, 1743
BseBI	CCWGG	4 cut(s)	308, 831, 1668, 1927
BseCI	ATCGAT	2 cut(s)	274, 1114
BseDI	CCNNGG	7 cut(s)	279, 553, 1034, 1299, 1833, 1925, 1926
BseGI	GGATG	5 cut(s)	247, 307, 658, 1420, 2038
BseLI	CCNNNNNNNGG	6 cut(s)	313, 1127, 1130, 1447, 1552, 2083
BseMII	CTCAG	3 cut(s)	523, 947, 1359
BseNI	ACTGG	5 cut(s)	338, 1153, 1540, 1636, 1743
BseRI	GAGGAG	2 cut(s)	329, 1763
BseSI	GKGCMC	2 cut(s)	1441, 1585
BseXI	GCAGC	5 cut(s)	508, 1093, 1300, 1307, 1804
BseYI	CCCAGC	2 cut(s)	1440, 1546
BsgI	GTGCAG	3 cut(s)	177, 1191, 1299
Bsh1236I	CGCG	2 cut(s)	13, 1356
Bsh1285I	CGRYCG	1 cut(s)	2030
BshFI	GGCC	7 cut(s)	36, 306, 546, 1249, 1406, 1832, 1873
BshNI	GGYRCC	1 cut(s)	1921
BshVI	ATCGAT	2 cut(s)	274, 1114
BsiEI	CGRYCG	1 cut(s)	2030
BsiHKAI	GWGCWC	2 cut(s)	1585, 1618
BsiHKCI	CYCGRG	1 cut(s)	890
BsiSI	CCGG	6 cut(s)	140, 443, 1128, 1639, 1878, 2030
BslFI	GGGAC	1 cut(s)	1658
BslI	CCNNNNNNNGG	6 cut(s)	313, 1127, 1130, 1447, 1552, 2083
BsmAI	GTCTC	7 cut(s)	117, 176, 333, 451, 820, 1002, 1749
BsmBI	CGTCTC	1 cut(s)	117
BsmFI	GGGAC	1 cut(s)	1658
BsmI	GAATGC	1 cut(s)	1820
BsnI	GGCC	7 cut(s)	36, 306, 546, 1249, 1406, 1832, 1873
Bso31I	GGTCTC	4 cut(s)	176, 333, 451, 820
BsoBI	CYCGRG	1 cut(s)	890
Bsp1286I	GDGCHC	3 cut(s)	1441, 1585, 1618
Bsp1407I	TGTACA	1 cut(s)	524
Bsp143I	GATC	6 cut(s)	275, 318, 886, 1327, 1555, 2137
Bsp1720I	GCTNAGC	1 cut(s)	1497
Bsp19I	CCATGG	4 cut(s)	279, 553, 1034, 1299
BspACI	CCGC	4 cut(s)	13, 33, 311, 1058
BspANI	GGCC	7 cut(s)	36, 306, 546, 1249, 1406, 1832, 1873
BspCNI	CTCAG	3 cut(s)	522, 946, 1358
BspDI	ATCGAT	2 cut(s)	274, 1114
BspFNI	CGCG	2 cut(s)	13, 1356
BspHI	TCATGA	1 cut(s)	883
BspLI	GGNNCC	7 cut(s)	559, 872, 1378, 1665, 1679, 1923, 2014
BspPI	GGATC	4 cut(s)	270, 313, 1322, 1563
BspQI	GCTCTTC	1 cut(s)	221
BspT107I	GGYRCC	1 cut(s)	1921
BspTI	CTTAAG	1 cut(s)	491
BspTNI	GGTCTC	4 cut(s)	176, 333, 451, 820
BsrFI	RCCGGY	1 cut(s)	1877
BsrGI	TGTACA	1 cut(s)	524
BsrI	ACTGG	5 cut(s)	338, 1153, 1540, 1636, 1743
BssAI	RCCGGY	1 cut(s)	1877
BssECI	CCNNGG	7 cut(s)	279, 553, 1034, 1299, 1833, 1925, 1926
BssMI	GATC	6 cut(s)	275, 318, 886, 1327, 1555, 2137
BssSI	CACGAG	1 cut(s)	1946
BssT1I	CCWWGG	4 cut(s)	279, 553, 1034, 1299
Bst2BI	CACGAG	1 cut(s)	1946
Bst2UI	CCWGG	4 cut(s)	308, 831, 1668, 1927
Bst4CI	ACNGT	4 cut(s)	133, 468, 721, 1336
Bst6I	CTCTTC	1 cut(s)	221
BstAFI	CTTAAG	1 cut(s)	491
BstAUI	TGTACA	1 cut(s)	524
BstBAI	YACGTR	1 cut(s)	725
BstC8I	GCNNGC	7 cut(s)	732, 836, 1047, 1099, 1494, 1875, 1879
BstDEI	CTNAG	8 cut(s)	509, 933, 1070, 1274, 1345, 1497, 1502, 2020
BstDSI	CCRYGG	4 cut(s)	279, 553, 1034, 1299
BstENI	CCTNNNNNAGG	2 cut(s)	311, 1128
BstF5I	GGATG	5 cut(s)	247, 307, 658, 1420, 2038
BstFNI	CGCG	2 cut(s)	13, 1356
BstHHI	GCGC	2 cut(s)	259, 1509
BstKTI	GATC	6 cut(s)	278, 321, 889, 1330, 1558, 2140
BstMAI	GTCTC	7 cut(s)	117, 176, 333, 451, 820, 1002, 1749
BstMBI	GATC	6 cut(s)	275, 318, 886, 1327, 1555, 2137
BstMCI	CGRYCG	1 cut(s)	2030
BstMWI	GCNNNNNNNGC	8 cut(s)	1055, 1103, 1301, 1310, 1498, 1792, 1817, 1919
BstNI	CCWGG	4 cut(s)	308, 831, 1668, 1927
BstNSI	RCATGY	3 cut(s)	838, 1049, 2042
BstSCI	CCNGG	5 cut(s)	306, 441, 829, 1666, 1925
BstSFI	CTRYAG	1 cut(s)	864
BstSLI	GKGCMC	2 cut(s)	1441, 1585
BstSNI	TACGTA	1 cut(s)	725
BstUI	CGCG	2 cut(s)	13, 1356
BstV1I	GCAGC	5 cut(s)	508, 1093, 1300, 1307, 1804
BstX2I	RGATCY	2 cut(s)	318, 1555
BstYI	RGATCY	2 cut(s)	318, 1555
Bsu15I	ATCGAT	2 cut(s)	274, 1114
BsuRI	GGCC	7 cut(s)	36, 306, 546, 1249, 1406, 1832, 1873
BsuTUI	ATCGAT	2 cut(s)	274, 1114
BtgI	CCRYGG	4 cut(s)	279, 553, 1034, 1299
BtgZI	GCGATG	2 cut(s)	1116, 1337
BtsCI	GGATG	5 cut(s)	247, 307, 658, 1420, 2038
BtsI	GCAGTG	1 cut(s)	1888
BtsIMutI	CAGTG	3 cut(s)	1353, 1736, 1888
Cac8I	GCNNGC	7 cut(s)	732, 836, 1047, 1099, 1494, 1875, 1879
CaiI	CAGNNNCTG	1 cut(s)	164
CciI	TCATGA	1 cut(s)	883
CfoI	GCGC	2 cut(s)	259, 1509
Cfr10I	RCCGGY	1 cut(s)	1877
Cfr13I	GGNCC	5 cut(s)	161, 871, 1404, 1625, 1741
ClaI	ATCGAT	2 cut(s)	274, 1114
CseI	GACGC	1 cut(s)	1345
Csp6I	GTAC	6 cut(s)	469, 525, 699, 717, 722, 1203
CviQI	GTAC	6 cut(s)	469, 525, 699, 717, 722, 1203
DdeI	CTNAG	8 cut(s)	509, 933, 1070, 1274, 1345, 1497, 1502, 2020
DpnI	GATC	6 cut(s)	277, 320, 888, 1329, 1557, 2139
DpnII	GATC	6 cut(s)	275, 318, 886, 1327, 1555, 2137
DrdI	GACNNNNNNGTC	2 cut(s)	168, 1632
DseDI	GACNNNNNNGTC	2 cut(s)	168, 1632
EaeI	YGGCCR	1 cut(s)	1830
Eam1104I	CTCTTC	1 cut(s)	221
EarI	CTCTTC	1 cut(s)	221
EciI	GGCGGA	1 cut(s)	326
Eco105I	TACGTA	1 cut(s)	725
Eco130I	CCWWGG	4 cut(s)	279, 553, 1034, 1299
Eco147I	AGGCCT	1 cut(s)	546
Eco31I	GGTCTC	4 cut(s)	176, 333, 451, 820
Eco32I	GATATC	1 cut(s)	1990
Eco47I	GGWCC	4 cut(s)	161, 871, 1625, 1741
Eco57I	CTGAAG	2 cut(s)	879, 1071
Eco88I	CYCGRG	1 cut(s)	890
EcoNI	CCTNNNNNAGG	2 cut(s)	311, 1128
EcoO109I	RGGNCCY	3 cut(s)	161, 871, 1625
EcoRI	GAATTC	2 cut(s)	1152, 1838
EcoRII	CCWGG	4 cut(s)	306, 829, 1666, 1925
EcoRV	GATATC	1 cut(s)	1990
EcoT14I	CCWWGG	4 cut(s)	279, 553, 1034, 1299
EcoT22I	ATGCAT	1 cut(s)	1047
ErhI	CCWWGG	4 cut(s)	279, 553, 1034, 1299
Esp3I	CGTCTC	1 cut(s)	117
FalI	AAGNNNNNCTT	2 cut(s)	1569, 1601
FaqI	GGGAC	1 cut(s)	1658
FauNDI	CATATG	1 cut(s)	408
Fnu4HI	GCNGC	6 cut(s)	34, 497, 1107, 1296, 1314, 1818
FokI	GGATG	5 cut(s)	234, 314, 665, 1427, 2045
Fsp4HI	GCNGC	6 cut(s)	34, 497, 1107, 1296, 1314, 1818
FspBI	CTAG	3 cut(s)	200, 1004, 1574
GlaI	GCGC	2 cut(s)	258, 1508
GluI	GCNGC	6 cut(s)	34, 497, 1107, 1296, 1314, 1818
GsaI	CCCAGC	2 cut(s)	1444, 1550
GsuI	CTGGAG	4 cut(s)	756, 1523, 1602, 1773
HaeIII	GGCC	7 cut(s)	36, 306, 546, 1249, 1406, 1832, 1873
HapII	CCGG	6 cut(s)	140, 443, 1128, 1639, 1878, 2030
HgaI	GACGC	1 cut(s)	1345
HhaI	GCGC	2 cut(s)	259, 1509
Hin6I	GCGC	2 cut(s)	257, 1507
HinP1I	GCGC	2 cut(s)	257, 1507
HincII	GTYRAC	1 cut(s)	403
HindII	GTYRAC	1 cut(s)	403
HindIII	AAGCTT	1 cut(s)	770
HinfI	GANTC	7 cut(s)	107, 244, 916, 1116, 1160, 1451, 1893
HpaII	CCGG	6 cut(s)	140, 443, 1128, 1639, 1878, 2030
HphI	GGTGA	2 cut(s)	764, 1956
Hpy166II	GTNNAC	3 cut(s)	403, 485, 1583
Hpy188I	TCNGA	7 cut(s)	106, 147, 532, 680, 1393, 1450, 1708
Hpy8I	GTNNAC	3 cut(s)	403, 485, 1583
HpyAV	CCTTC	6 cut(s)	536, 1514, 1571, 1616, 1665, 2088
HpyCH4III	ACNGT	4 cut(s)	133, 468, 721, 1336
HpyCH4IV	ACGT	3 cut(s)	724, 1243, 1692
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1055, 1103, 1301, 1310, 1498, 1792, 1817, 1919
HpyF3I	CTNAG	8 cut(s)	509, 933, 1070, 1274, 1345, 1497, 1502, 2020
HpySE526I	ACGT	3 cut(s)	724, 1243, 1692
HspAI	GCGC	2 cut(s)	257, 1507
KroI	GCCGGC	1 cut(s)	1877
KroNI	GCCGGC	1 cut(s)	1879
Kzo9I	GATC	6 cut(s)	275, 318, 886, 1327, 1555, 2137
LguI	GCTCTTC	1 cut(s)	221
LmnI	GCTCC	4 cut(s)	707, 1621, 1683, 2012
Lsp1109I	GCAGC	5 cut(s)	508, 1093, 1300, 1307, 1804
LweI	GCATC	4 cut(s)	647, 1093, 1360, 1922
MaeI	CTAG	3 cut(s)	200, 1004, 1574
MaeII	ACGT	3 cut(s)	724, 1243, 1692
MaeIII	GTNAC	4 cut(s)	631, 1723, 1962, 2072
MalI	GATC	6 cut(s)	277, 320, 888, 1329, 1557, 2139
MboI	GATC	6 cut(s)	275, 318, 886, 1327, 1555, 2137
MboII	GAAGA	5 cut(s)	238, 841, 959, 973, 1529
MflI	RGATCY	2 cut(s)	318, 1555
MhlI	GDGCHC	3 cut(s)	1441, 1585, 1618
MlyI	GAGTC	3 cut(s)	101, 1154, 1902
MmeI	TCCRAC	3 cut(s)	519, 1355, 1558
Mph1103I	ATGCAT	1 cut(s)	1047
MroNI	GCCGGC	1 cut(s)	1877
MseI	TTAA	6 cut(s)	191, 492, 581, 1224, 1887, 1956
MslI	CAYNNNNRTG	5 cut(s)	284, 594, 1016, 1691, 1981
MspCI	CTTAAG	1 cut(s)	491
MspI	CCGG	6 cut(s)	140, 443, 1128, 1639, 1878, 2030
MspR9I	CCNGG	5 cut(s)	308, 443, 831, 1668, 1927
Mva1269I	GAATGC	1 cut(s)	1820
MvaI	CCWGG	4 cut(s)	308, 831, 1668, 1927
MvnI	CGCG	2 cut(s)	13, 1356
MwoI	GCNNNNNNNGC	8 cut(s)	1055, 1103, 1301, 1310, 1498, 1792, 1817, 1919
NaeI	GCCGGC	1 cut(s)	1879
NciI	CCSGG	1 cut(s)	443
NcoI	CCATGG	4 cut(s)	279, 553, 1034, 1299
NdeI	CATATG	1 cut(s)	408
NdeII	GATC	6 cut(s)	275, 318, 886, 1327, 1555, 2137
NgoMIV	GCCGGC	1 cut(s)	1877
NlaIV	GGNNCC	7 cut(s)	559, 872, 1378, 1665, 1679, 1923, 2014
NmeAIII	GCCGAG	3 cut(s)	1858, 2084, 2094
NmuCI	GTSAC	3 cut(s)	631, 1962, 2072
NsiI	ATGCAT	1 cut(s)	1047
NspI	RCATGY	3 cut(s)	838, 1049, 2042
PaeI	GCATGC	2 cut(s)	838, 1049
PaeR7I	CTCGAG	1 cut(s)	890
PagI	TCATGA	1 cut(s)	883
PasI	CCCWGGG	1 cut(s)	1926
PceI	AGGCCT	1 cut(s)	546
PciSI	GCTCTTC	1 cut(s)	221
PctI	GAATGC	1 cut(s)	1820
PdiI	GCCGGC	1 cut(s)	1879
PfeI	GAWTC	4 cut(s)	244, 916, 1116, 1451
PflFI	GACNNNGTC	1 cut(s)	1760
PkrI	GCNGC	6 cut(s)	35, 498, 1108, 1297, 1315, 1819
PleI	GAGTC	3 cut(s)	101, 1154, 1901
PpsI	GAGTC	3 cut(s)	101, 1154, 1901
Ppu21I	YACGTR	1 cut(s)	725
PpuMI	RGGWCCY	3 cut(s)	161, 871, 1625
PshBI	ATTAAT	1 cut(s)	581
Psp5II	RGGWCCY	3 cut(s)	161, 871, 1625
Psp6I	CCWGG	4 cut(s)	306, 829, 1666, 1925
PspFI	CCCAGC	2 cut(s)	1440, 1546
PspGI	CCWGG	4 cut(s)	306, 829, 1666, 1925
PspN4I	GGNNCC	7 cut(s)	559, 872, 1378, 1665, 1679, 1923, 2014
PspPI	GGNCC	5 cut(s)	161, 871, 1404, 1625, 1741
PspPPI	RGGWCCY	3 cut(s)	161, 871, 1625
PstNI	CAGNNNCTG	1 cut(s)	164
PsuI	RGATCY	2 cut(s)	318, 1555
PsyI	GACNNNGTC	1 cut(s)	1760
RsaI	GTAC	6 cut(s)	470, 526, 700, 718, 723, 1204
RsaNI	GTAC	6 cut(s)	469, 525, 699, 717, 722, 1203
RseI	CAYNNNNRTG	5 cut(s)	284, 594, 1016, 1691, 1981
SapI	GCTCTTC	1 cut(s)	221
SaqAI	TTAA	6 cut(s)	191, 492, 581, 1224, 1887, 1956
SatI	GCNGC	6 cut(s)	34, 497, 1107, 1296, 1314, 1818
Sau3AI	GATC	6 cut(s)	275, 318, 886, 1327, 1555, 2137
Sau96I	GGNCC	5 cut(s)	161, 871, 1404, 1625, 1741
ScaI	AGTACT	1 cut(s)	718
SchI	GAGTC	3 cut(s)	101, 1154, 1902
ScrFI	CCNGG	5 cut(s)	308, 443, 831, 1668, 1927
SduI	GDGCHC	3 cut(s)	1441, 1585, 1618
SfaNI	GCATC	4 cut(s)	647, 1093, 1360, 1922
SfcI	CTRYAG	1 cut(s)	864
Sfr274I	CTCGAG	1 cut(s)	890
SinI	GGWCC	4 cut(s)	161, 871, 1625, 1741
SlaI	CTCGAG	1 cut(s)	890
SmiMI	CAYNNNNRTG	5 cut(s)	284, 594, 1016, 1691, 1981
SmlI	CTYRAG	5 cut(s)	491, 647, 790, 890, 1714
SmoI	CTYRAG	5 cut(s)	491, 647, 790, 890, 1714
SnaBI	TACGTA	1 cut(s)	725
SphI	GCATGC	2 cut(s)	838, 1049
SseBI	AGGCCT	1 cut(s)	546
SsiI	CCGC	4 cut(s)	13, 33, 311, 1058
SspMI	CTAG	3 cut(s)	200, 1004, 1574
StuI	AGGCCT	1 cut(s)	546
StyD4I	CCNGG	5 cut(s)	306, 441, 829, 1666, 1925
StyI	CCWWGG	4 cut(s)	279, 553, 1034, 1299
TaaI	ACNGT	4 cut(s)	133, 468, 721, 1336
TaiI	ACGT	3 cut(s)	727, 1246, 1695
TaqI	TCGA	8 cut(s)	242, 274, 891, 997, 1114, 1764, 1896, 2026
TatI	WGTACW	3 cut(s)	468, 524, 716
TauI	GCSGC	1 cut(s)	36
TfiI	GAWTC	4 cut(s)	244, 916, 1116, 1451
Tru1I	TTAA	6 cut(s)	191, 492, 581, 1224, 1887, 1956
Tru9I	TTAA	6 cut(s)	191, 492, 581, 1224, 1887, 1956
TscAI	CASTG	3 cut(s)	1353, 1743, 1888
TseFI	GTSAC	3 cut(s)	631, 1962, 2072
TseI	GCWGC	5 cut(s)	496, 1106, 1295, 1313, 1817
Tsp45I	GTSAC	3 cut(s)	631, 1962, 2072
TspDTI	ATGAA	8 cut(s)	253, 349, 835, 1157, 1248, 1479, 1703, 2015
TspGWI	ACGGA	3 cut(s)	909, 2060, 2099
TspRI	CASTG	3 cut(s)	1353, 1743, 1888
Tth111I	GACNNNGTC	1 cut(s)	1760
Vha464I	CTTAAG	1 cut(s)	491
VneI	GTGCAC	1 cut(s)	1581
VpaK11BI	GGWCC	4 cut(s)	161, 871, 1625, 1741
VspI	ATTAAT	1 cut(s)	581
XagI	CCTNNNNNAGG	2 cut(s)	311, 1128
XapI	RAATTY	5 cut(s)	902, 1152, 1419, 1838, 1969
XbaI	TCTAGA	1 cut(s)	1573
XceI	RCATGY	3 cut(s)	838, 1049, 2042
XcmI	CCANNNNNNNNNTGG	1 cut(s)	345
XhoI	CTCGAG	1 cut(s)	890
XspI	CTAG	3 cut(s)	200, 1004, 1574
ZrmI	AGTACT	1 cut(s)	718
Zsp2I	ATGCAT	1 cut(s)	1047

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.