FvH4_4g24540

NADH-ubiquinone reductase complex 1 MLRQ subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004480
Physical Location & Seq
Forward (+)
26652409 .. 26654380
1972 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24540.t1

Sequence Viewer

Length: 264 bp
ATGGCTAGCAGATGGATCAGGCCCGAGGTGTACCCGCTCTTTGCAGCAACTGGTGTTGCTGTTGGTATCTGTGCAATGCAACTCGTTAGGAACATCACTGGCAACCCTGAAGTCAGGGTTACAAAGGAGAACAGAGCTGCTGGAATTCTAGACAACTTTGAAGAGGGTGAGAAATACAGAGAAAATGCTGTTAGGAGGTTTGTCCGCAACAAAAAGCCCCAGATCATGCCATCCATCAACAATTTTTTCTCAGAACCAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B12D

Protein Analysis

88

Amino Acids

9.93

Weight (kDa)

9.73

Isoelectric Point (pI)

48.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B12D PF06522 5 - 59 4.8e-23 NADH-ubiquinone reductase complex 1 MLRQ subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 37
AciI CCGC 2 cut(s) 35, 205
AclWI GGATC 1 cut(s) 23
AcsI RAATTY 1 cut(s) 144
AcuI CTGAAG 1 cut(s) 129
AfaI GTAC 1 cut(s) 32
AgsI TTSAA 1 cut(s) 161
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
AlwI GGATC 1 cut(s) 23
AlwNI CAGNNNCTG 1 cut(s) 50
Ama87I CYCGRG 1 cut(s) 23
AoxI GGCC 1 cut(s) 20
ApeKI GCWGC 2 cut(s) 44, 137
ApoI RAATTY 1 cut(s) 144
AspS9I GGNCC 1 cut(s) 21
AsuHPI GGTGA 1 cut(s) 179
AsuNHI GCTAGC 1 cut(s) 5
AvaI CYCGRG 1 cut(s) 23
BbvI GCAGC 2 cut(s) 56, 124
BccI CCATC 3 cut(s) 6, 238, 242
BfaI CTAG 2 cut(s) 6, 149
BisI GCNGC 2 cut(s) 45, 138
BlsI GCNGC 2 cut(s) 46, 139
BmeT110I CYCGRG 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 21
BmtI GCTAGC 1 cut(s) 9
BsaJI CCNNGG 1 cut(s) 24
Bse1I ACTGG 2 cut(s) 55, 103
Bse3DI GCAATG 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 24
BseGI GGATG 1 cut(s) 230
BseMI GCAATG 1 cut(s) 81
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 2 cut(s) 55, 103
BseXI GCAGC 2 cut(s) 56, 124
BshFI GGCC 1 cut(s) 22
BsiHKCI CYCGRG 1 cut(s) 23
BsnI GGCC 1 cut(s) 22
BsoBI CYCGRG 1 cut(s) 23
Bsp143I GATC 2 cut(s) 15, 222
BspACI CCGC 2 cut(s) 35, 205
BspANI GGCC 1 cut(s) 22
BspCNI CTCAG 1 cut(s) 263
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 1 cut(s) 23
BsrBI CCGCTC 1 cut(s) 37
BsrDI GCAATG 1 cut(s) 81
BsrI ACTGG 2 cut(s) 55, 103
BssECI CCNNGG 1 cut(s) 24
BssMI GATC 2 cut(s) 15, 222
Bst6I CTCTTC 1 cut(s) 156
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 1 cut(s) 250
BstF5I GGATG 1 cut(s) 230
BstKTI GATC 2 cut(s) 18, 225
BstMBI GATC 2 cut(s) 15, 222
BstV1I GCAGC 2 cut(s) 56, 124
BsuRI GGCC 1 cut(s) 22
BtsCI GGATG 1 cut(s) 230
BtsIMutI CAGTG 1 cut(s) 96
Cac8I GCNNGC 1 cut(s) 7
CaiI CAGNNNCTG 1 cut(s) 50
Cfr13I GGNCC 1 cut(s) 21
Csp6I GTAC 1 cut(s) 31
CviAII CATG 1 cut(s) 226
CviJI RGCY 4 cut(s) 5, 22, 137, 217
CviKI_1 RGCY 4 cut(s) 5, 22, 137, 217
CviQI GTAC 1 cut(s) 31
DdeI CTNAG 1 cut(s) 250
DpnI GATC 2 cut(s) 17, 224
DpnII GATC 2 cut(s) 15, 222
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
Eco57I CTGAAG 1 cut(s) 129
Eco88I CYCGRG 1 cut(s) 23
EcoRI GAATTC 1 cut(s) 144
FaeI CATG 1 cut(s) 229
FaiI YATR 1 cut(s) 227
FatI CATG 1 cut(s) 225
FauI CCCGC 1 cut(s) 42
Fnu4HI GCNGC 2 cut(s) 45, 138
FokI GGATG 1 cut(s) 217
Fsp4HI GCNGC 2 cut(s) 45, 138
FspBI CTAG 2 cut(s) 6, 149
GluI GCNGC 2 cut(s) 45, 138
HaeIII GGCC 1 cut(s) 22
Hin1II CATG 1 cut(s) 229
HphI GGTGA 1 cut(s) 179
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 1 cut(s) 253
Hpy188III TCNNGA 1 cut(s) 149
Hpy8I GTNNAC 1 cut(s) 31
HpyCH4V TGCA 3 cut(s) 44, 74, 79
HpyF3I CTNAG 1 cut(s) 250
Hsp92II CATG 1 cut(s) 229
Kzo9I GATC 2 cut(s) 15, 222
LpnPI CCDG 7 cut(s) 4, 36, 84, 100, 120, 126, 233
Lsp1109I GCAGC 2 cut(s) 56, 124
MaeI CTAG 2 cut(s) 6, 149
MaeIII GTNAC 1 cut(s) 118
MalI GATC 2 cut(s) 17, 224
MbiI CCGCTC 1 cut(s) 37
MboI GATC 2 cut(s) 15, 222
MboII GAAGA 1 cut(s) 173
MluCI AATT 2 cut(s) 144, 241
MnlI CCTC 3 cut(s) 19, 157, 189
NdeII GATC 2 cut(s) 15, 222
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 1 cut(s) 229
PkrI GCNGC 2 cut(s) 46, 139
PspPI GGNCC 1 cut(s) 21
PstNI CAGNNNCTG 1 cut(s) 50
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
SatI GCNGC 2 cut(s) 45, 138
Sau3AI GATC 2 cut(s) 15, 222
Sau96I GGNCC 1 cut(s) 21
SetI ASST 3 cut(s) 30, 139, 200
Sse9I AATT 2 cut(s) 144, 241
SsiI CCGC 2 cut(s) 35, 205
SspMI CTAG 2 cut(s) 6, 149
TasI AATT 2 cut(s) 144, 241
TscAI CASTG 1 cut(s) 103
TseI GCWGC 2 cut(s) 44, 137
TspRI CASTG 1 cut(s) 103
XapI RAATTY 1 cut(s) 144
XbaI TCTAGA 1 cut(s) 148
XspI CTAG 2 cut(s) 6, 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.