Prupe.5G180300_v2.0.a1

B12D protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0004480
Physical Location & Seq
Reverse (-)
15135308 .. 15137080
1773 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G180300.1

Sequence Viewer

Length: 273 bp
ATGGCTCCGAATCGTTGGTTGAGGCCTGAGGTTTATCCACTGTTCGCGGCCACAGGGGTTGCCGTAGGGATTTGCGGGCTTAGCTTGTTCCGCCATATCACAATCAACCCGGAAGTCAGAGTGAGCAAGGAGAACAGGGCTGCTGGAATATTGGAGAATTTCGACGAGGGAGAAAAGTACAAGGAGCATTTCCTGAGGAAATTTGTCCGCAACAAGTCGCCAGAAATTATGCCGTCCGTCAATGGTTTTTTCACAGATCCAAGCCGGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B12D

Protein Analysis

91

Amino Acids

10.28

Weight (kDa)

9.36

Isoelectric Point (pI)

57.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 47
AciI CCGC 4 cut(s) 47, 75, 91, 208
AclWI GGATC 1 cut(s) 251
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 2 cut(s) 157, 200
AfaI GTAC 1 cut(s) 179
AluBI AGCT 1 cut(s) 84
AluI AGCT 1 cut(s) 84
AlwI GGATC 1 cut(s) 251
AoxI GGCC 2 cut(s) 23, 48
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 2 cut(s) 157, 200
AsuC2I CCSGG 1 cut(s) 110
AxyI CCTNAGG 2 cut(s) 27, 194
BbvI GCAGC 1 cut(s) 127
BceAI ACGGC 2 cut(s) 47, 217
BcnI CCSGG 1 cut(s) 110
BisI GCNGC 2 cut(s) 48, 141
BlpI GCTNAGC 1 cut(s) 80
BlsI GCNGC 2 cut(s) 49, 142
Bme1390I CCNGG 1 cut(s) 110
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 110
Bpu1102I GCTNAGC 1 cut(s) 80
BpuMI CCSGG 1 cut(s) 110
Bse21I CCTNAGG 2 cut(s) 27, 194
BseMII CTCAG 2 cut(s) 18, 185
BseXI GCAGC 1 cut(s) 127
Bsh1236I CGCG 1 cut(s) 47
BshFI GGCC 2 cut(s) 25, 50
BsiSI CCGG 2 cut(s) 110, 265
BsnI GGCC 2 cut(s) 25, 50
Bsp143I GATC 1 cut(s) 256
Bsp1720I GCTNAGC 1 cut(s) 80
BspACI CCGC 4 cut(s) 47, 75, 91, 208
BspANI GGCC 2 cut(s) 25, 50
BspCNI CTCAG 2 cut(s) 19, 186
BspFNI CGCG 1 cut(s) 47
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 251
BssMI GATC 1 cut(s) 256
Bst4CI ACNGT 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 77
BstDEI CTNAG 3 cut(s) 27, 80, 194
BstFNI CGCG 1 cut(s) 47
BstKTI GATC 1 cut(s) 259
BstMBI GATC 1 cut(s) 256
BstMWI GCNNNNNNNGC 2 cut(s) 81, 90
BstSCI CCNGG 1 cut(s) 108
BstUI CGCG 1 cut(s) 47
BstV1I GCAGC 1 cut(s) 127
BstX2I RGATCY 1 cut(s) 256
BstYI RGATCY 1 cut(s) 256
Bsu36I CCTNAGG 2 cut(s) 27, 194
BsuRI GGCC 2 cut(s) 25, 50
BtsIMutI CAGTG 1 cut(s) 38
Cac8I GCNNGC 1 cut(s) 77
Csp6I GTAC 1 cut(s) 178
CspCI CAANNNNNGTGG 2 cut(s) 40, 75
CviJI RGCY 7 cut(s) 5, 25, 50, 79, 84, 140, 264
CviKI_1 RGCY 7 cut(s) 5, 25, 50, 79, 84, 140, 264
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 3 cut(s) 27, 80, 194
DpnI GATC 1 cut(s) 258
DpnII GATC 1 cut(s) 256
EaeI YGGCCR 1 cut(s) 48
EciI GGCGGA 1 cut(s) 80
Eco147I AGGCCT 1 cut(s) 25
Eco81I CCTNAGG 2 cut(s) 27, 194
FaiI YATR 2 cut(s) 96, 230
FauI CCCGC 1 cut(s) 68
Fnu4HI GCNGC 2 cut(s) 48, 141
Fsp4HI GCNGC 2 cut(s) 48, 141
GluI GCNGC 2 cut(s) 48, 141
HaeIII GGCC 2 cut(s) 25, 50
HapII CCGG 2 cut(s) 110, 265
HinfI GANTC 1 cut(s) 10
HpaII CCGG 2 cut(s) 110, 265
Hpy188I TCNGA 2 cut(s) 9, 119
Hpy188III TCNNGA 1 cut(s) 193
Hpy99I CGWCG 1 cut(s) 167
HpyCH4III ACNGT 1 cut(s) 42
HpyF10VI GCNNNNNNNGC 2 cut(s) 81, 90
HpyF3I CTNAG 3 cut(s) 27, 80, 194
Kzo9I GATC 1 cut(s) 256
LmnI GCTCC 2 cut(s) 10, 184
LpnPI CCDG 7 cut(s) 39, 39, 121, 123, 129, 206, 234
Lsp1109I GCAGC 1 cut(s) 127
MalI GATC 1 cut(s) 258
MboI GATC 1 cut(s) 256
MflI RGATCY 1 cut(s) 256
MluCI AATT 4 cut(s) 157, 200, 225, 268
MnlI CCTC 4 cut(s) 15, 22, 160, 189
MseI TTAA 1 cut(s) 271
MspI CCGG 2 cut(s) 110, 265
MspR9I CCNGG 1 cut(s) 110
MvnI CGCG 1 cut(s) 47
MwoI GCNNNNNNNGC 2 cut(s) 81, 90
NciI CCSGG 1 cut(s) 110
NdeII GATC 1 cut(s) 256
NlaIV GGNNCC 1 cut(s) 6
PceI AGGCCT 1 cut(s) 25
PfeI GAWTC 1 cut(s) 10
PkrI GCNGC 2 cut(s) 49, 142
PspN4I GGNNCC 1 cut(s) 6
PsuI RGATCY 1 cut(s) 256
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
SaqAI TTAA 1 cut(s) 271
SatI GCNGC 2 cut(s) 48, 141
Sau3AI GATC 1 cut(s) 256
ScrFI CCNGG 1 cut(s) 110
SetI ASST 2 cut(s) 33, 86
Sse9I AATT 4 cut(s) 157, 200, 225, 268
SseBI AGGCCT 1 cut(s) 25
SsiI CCGC 4 cut(s) 47, 75, 91, 208
SspI AATATT 1 cut(s) 150
StuI AGGCCT 1 cut(s) 25
StyD4I CCNGG 1 cut(s) 108
TaaI ACNGT 1 cut(s) 42
TaqI TCGA 1 cut(s) 162
TasI AATT 4 cut(s) 157, 200, 225, 268
TatI WGTACW 1 cut(s) 177
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 1 cut(s) 10
Tru1I TTAA 1 cut(s) 271
Tru9I TTAA 1 cut(s) 271
TscAI CASTG 1 cut(s) 45
TseI GCWGC 1 cut(s) 140
TspGWI ACGGA 1 cut(s) 226
TspRI CASTG 1 cut(s) 45
XapI RAATTY 2 cut(s) 157, 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.