MD14G1188100.v1.1

NADH-ubiquinone reductase complex 1 MLRQ subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004480
Physical Location & Seq
Reverse (-)
28000184 .. 28002548
2365 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1188100.v1.1.491

Sequence Viewer

Length: 273 bp
ATGGCTGCAAATCGCTGGCTGAGGCCCGAGGTTTATCCACTGTTCGCGGCCACAGGGGTCGCCGTAGGGATCTGCGGGTTGAGTCTGTTCCGCCATATCACCATCAACCCTGAAGTCAGAGTGAGCAAGGAGGGCAGAGCTGCTGGAGTATTGGATAATCACGCCGAAGGAGAAAAGTACAAAGAGAATTTCTTGAGGAAATTTGTCCGCAACAGGGCACCGGAGATTATGCCAGGCATCAACGGTTTCTTCACGGACCCGAAAAATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B12D

Protein Analysis

91

Amino Acids

10.09

Weight (kDa)

9.65

Isoelectric Point (pI)

41.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B12D PF06522 6 - 60 5.6e-21 NADH-ubiquinone reductase complex 1 MLRQ subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 217
AccII CGCG 1 cut(s) 47
AciI CCGC 4 cut(s) 47, 75, 91, 208
AclWI GGATC 1 cut(s) 77
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 2 cut(s) 187, 200
AcuI CTGAAG 1 cut(s) 132
AfaI GTAC 1 cut(s) 179
AfiI CCNNNNNNNGG 1 cut(s) 214
AjnI CCWGG 1 cut(s) 232
AluBI AGCT 1 cut(s) 140
AluI AGCT 1 cut(s) 140
AlwI GGATC 1 cut(s) 77
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 2 cut(s) 23, 48
ApeKI GCWGC 2 cut(s) 5, 140
ApoI RAATTY 2 cut(s) 187, 200
AspS9I GGNCC 2 cut(s) 24, 256
AsuHPI GGTGA 1 cut(s) 91
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 1 cut(s) 256
BaeGI GKGCMC 1 cut(s) 220
BanI GGYRCC 1 cut(s) 217
BbvCI CCTCAGC 1 cut(s) 20
BbvI GCAGC 1 cut(s) 127
BccI CCATC 1 cut(s) 110
BceAI ACGGC 1 cut(s) 47
BciT130I CCWGG 1 cut(s) 234
BisI GCNGC 3 cut(s) 6, 48, 141
BlsI GCNGC 3 cut(s) 7, 49, 142
Bme1390I CCNGG 1 cut(s) 234
Bme18I GGWCC 1 cut(s) 256
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 2 cut(s) 24, 256
BmiI GGNNCC 2 cut(s) 219, 258
BmrFI CCNGG 1 cut(s) 234
BmsI GCATC 1 cut(s) 246
BpmI CTGGAG 1 cut(s) 165
Bpu10I CCTNAGC 1 cut(s) 20
BpuEI CTTGAG 1 cut(s) 214
BsaJI CCNNGG 1 cut(s) 27
BsaWI WCCGGW 1 cut(s) 220
Bsc4I CCNNNNNNNGG 1 cut(s) 214
BseBI CCWGG 1 cut(s) 234
BseDI CCNNGG 1 cut(s) 27
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMII CTCAG 1 cut(s) 11
BseSI GKGCMC 1 cut(s) 220
BseXI GCAGC 1 cut(s) 127
Bsh1236I CGCG 1 cut(s) 47
BshFI GGCC 2 cut(s) 25, 50
BshNI GGYRCC 1 cut(s) 217
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 1 cut(s) 221
BslI CCNNNNNNNGG 1 cut(s) 214
BsnI GGCC 2 cut(s) 25, 50
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 220
Bsp143I GATC 1 cut(s) 69
BspACI CCGC 4 cut(s) 47, 75, 91, 208
BspANI GGCC 2 cut(s) 25, 50
BspCNI CTCAG 1 cut(s) 12
BspFNI CGCG 1 cut(s) 47
BspLI GGNNCC 2 cut(s) 219, 258
BspPI GGATC 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 217
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 1 cut(s) 69
Bst2UI CCWGG 1 cut(s) 234
Bst4CI ACNGT 2 cut(s) 42, 245
BstC8I GCNNGC 1 cut(s) 17
BstDEI CTNAG 1 cut(s) 20
BstFNI CGCG 1 cut(s) 47
BstKTI GATC 1 cut(s) 72
BstMBI GATC 1 cut(s) 69
BstMWI GCNNNNNNNGC 1 cut(s) 132
BstNI CCWGG 1 cut(s) 234
BstSCI CCNGG 1 cut(s) 232
BstSLI GKGCMC 1 cut(s) 220
BstUI CGCG 1 cut(s) 47
BstV1I GCAGC 1 cut(s) 127
BstX2I RGATCY 1 cut(s) 69
BstYI RGATCY 1 cut(s) 69
BsuRI GGCC 2 cut(s) 25, 50
BtsIMutI CAGTG 1 cut(s) 38
Cac8I GCNNGC 1 cut(s) 17
Cfr13I GGNCC 2 cut(s) 24, 256
Csp6I GTAC 1 cut(s) 178
CviJI RGCY 5 cut(s) 5, 19, 25, 50, 140
CviKI_1 RGCY 5 cut(s) 5, 19, 25, 50, 140
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 1 cut(s) 20
DpnI GATC 1 cut(s) 71
DpnII GATC 1 cut(s) 69
EaeI YGGCCR 1 cut(s) 48
EciI GGCGGA 1 cut(s) 80
Eco47I GGWCC 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 132
Eco88I CYCGRG 1 cut(s) 26
EcoRII CCWGG 1 cut(s) 232
FaiI YATR 2 cut(s) 96, 230
FauI CCCGC 1 cut(s) 68
Fnu4HI GCNGC 3 cut(s) 6, 48, 141
Fsp4HI GCNGC 3 cut(s) 6, 48, 141
GluI GCNGC 3 cut(s) 6, 48, 141
GsuI CTGGAG 1 cut(s) 165
HaeIII GGCC 2 cut(s) 25, 50
HapII CCGG 1 cut(s) 221
HinfI GANTC 1 cut(s) 82
HpaII CCGG 1 cut(s) 221
HphI GGTGA 1 cut(s) 91
Hpy188I TCNGA 1 cut(s) 119
Hpy188III TCNNGA 1 cut(s) 193
HpyAV CCTTC 1 cut(s) 161
HpyCH4III ACNGT 2 cut(s) 42, 245
HpyCH4V TGCA 1 cut(s) 8
HpyF10VI GCNNNNNNNGC 1 cut(s) 132
HpyF3I CTNAG 1 cut(s) 20
Kzo9I GATC 1 cut(s) 69
LpnPI CCDG 7 cut(s) 39, 123, 129, 199, 219, 234, 246
Lsp1109I GCAGC 1 cut(s) 127
LweI GCATC 1 cut(s) 246
MalI GATC 1 cut(s) 71
MboI GATC 1 cut(s) 69
MboII GAAGA 1 cut(s) 241
MflI RGATCY 1 cut(s) 69
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 3 cut(s) 187, 200, 268
MlyI GAGTC 1 cut(s) 91
MnlI CCTC 4 cut(s) 15, 22, 124, 189
MspI CCGG 1 cut(s) 221
MspR9I CCNGG 1 cut(s) 234
MvaI CCWGG 1 cut(s) 234
MvnI CGCG 1 cut(s) 47
MwoI GCNNNNNNNGC 1 cut(s) 132
NdeII GATC 1 cut(s) 69
NlaIV GGNNCC 2 cut(s) 219, 258
PkrI GCNGC 3 cut(s) 7, 49, 142
PleI GAGTC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 232
PspGI CCWGG 1 cut(s) 232
PspN4I GGNNCC 2 cut(s) 219, 258
PspPI GGNCC 2 cut(s) 24, 256
PsuI RGATCY 1 cut(s) 69
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
SatI GCNGC 3 cut(s) 6, 48, 141
Sau3AI GATC 1 cut(s) 69
Sau96I GGNCC 2 cut(s) 24, 256
SchI GAGTC 1 cut(s) 91
ScrFI CCNGG 1 cut(s) 234
SduI GDGCHC 1 cut(s) 220
SetI ASST 2 cut(s) 33, 142
SfaNI GCATC 1 cut(s) 246
SinI GGWCC 1 cut(s) 256
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
Sse9I AATT 3 cut(s) 187, 200, 268
SsiI CCGC 4 cut(s) 47, 75, 91, 208
StyD4I CCNGG 1 cut(s) 232
TaaI ACNGT 2 cut(s) 42, 245
TasI AATT 3 cut(s) 187, 200, 268
TatI WGTACW 1 cut(s) 177
TauI GCSGC 1 cut(s) 50
TscAI CASTG 1 cut(s) 45
TseI GCWGC 2 cut(s) 5, 140
TspGWI ACGGA 1 cut(s) 269
TspRI CASTG 1 cut(s) 45
VpaK11BI GGWCC 1 cut(s) 256
XapI RAATTY 2 cut(s) 187, 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.