MD06G1182100.v1.1

NADH-ubiquinone reductase complex 1 MLRQ subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004480
Physical Location & Seq
Reverse (-)
32096592 .. 32098071
1480 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1182100.v1.1.491

Sequence Viewer

Length: 273 bp
ATGGCTGCGAATCGCTGGCTGAGGCCCGAGGTTTATCCACTGTTCGCTGCCACAGGGGTCGCCGTAGGGATCTGCGGGTTGAGTCTGTTCCGCCATATCACCATCAACCCTGAAGTCAGAGTGACCAAGGAGAACAGAGCTGCTGGAGTATTGGACAATCACGCCGAGGGAGAAAAGTACAAGGAGCATTTCCTGAGGAAATTCGTCCGCAACAAGGCACCGGAAATTATGCCAGGCATCAACGCTTTCTTCACCGACCCGAGCAAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B12D

Protein Analysis

91

Amino Acids

10.14

Weight (kDa)

9.58

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B12D PF06522 6 - 60 2.5e-21 NADH-ubiquinone reductase complex 1 MLRQ subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 217
AciI CCGC 3 cut(s) 75, 91, 208
AclWI GGATC 1 cut(s) 77
AcsI RAATTY 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 132
AfaI GTAC 1 cut(s) 179
AfiI CCNNNNNNNGG 1 cut(s) 214
AjnI CCWGG 1 cut(s) 232
AluBI AGCT 1 cut(s) 140
AluI AGCT 1 cut(s) 140
AlwI GGATC 1 cut(s) 77
Ama87I CYCGRG 2 cut(s) 26, 259
AoxI GGCC 1 cut(s) 23
ApeKI GCWGC 3 cut(s) 5, 47, 140
ApoI RAATTY 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 24
AsuHPI GGTGA 2 cut(s) 91, 244
AvaI CYCGRG 2 cut(s) 26, 259
AxyI CCTNAGG 1 cut(s) 194
BanI GGYRCC 1 cut(s) 217
BbvCI CCTCAGC 1 cut(s) 20
BbvI GCAGC 2 cut(s) 34, 127
BccI CCATC 1 cut(s) 110
BceAI ACGGC 1 cut(s) 47
BciT130I CCWGG 1 cut(s) 234
BisI GCNGC 3 cut(s) 6, 48, 141
BlsI GCNGC 3 cut(s) 7, 49, 142
Bme1390I CCNGG 1 cut(s) 234
BmeT110I CYCGRG 2 cut(s) 26, 259
BmgT120I GGNCC 1 cut(s) 24
BmiI GGNNCC 1 cut(s) 219
BmrFI CCNGG 1 cut(s) 234
BmsI GCATC 1 cut(s) 246
BpmI CTGGAG 1 cut(s) 165
Bpu10I CCTNAGC 1 cut(s) 20
BsaJI CCNNGG 3 cut(s) 27, 126, 165
BsaWI WCCGGW 1 cut(s) 220
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse21I CCTNAGG 1 cut(s) 194
BseBI CCWGG 1 cut(s) 234
BseDI CCNNGG 3 cut(s) 27, 126, 165
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMII CTCAG 2 cut(s) 11, 185
BseXI GCAGC 2 cut(s) 34, 127
BshFI GGCC 1 cut(s) 25
BshNI GGYRCC 1 cut(s) 217
BsiHKCI CYCGRG 2 cut(s) 26, 259
BsiSI CCGG 1 cut(s) 221
BslI CCNNNNNNNGG 1 cut(s) 214
BsnI GGCC 1 cut(s) 25
BsoBI CYCGRG 2 cut(s) 26, 259
Bsp143I GATC 1 cut(s) 69
BspACI CCGC 3 cut(s) 75, 91, 208
BspANI GGCC 1 cut(s) 25
BspCNI CTCAG 2 cut(s) 12, 186
BspLI GGNNCC 1 cut(s) 219
BspPI GGATC 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 217
BssECI CCNNGG 3 cut(s) 27, 126, 165
BssMI GATC 1 cut(s) 69
BssT1I CCWWGG 1 cut(s) 126
Bst2UI CCWGG 1 cut(s) 234
Bst4CI ACNGT 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 17
BstDEI CTNAG 2 cut(s) 20, 194
BstKTI GATC 1 cut(s) 72
BstMBI GATC 1 cut(s) 69
BstNI CCWGG 1 cut(s) 234
BstSCI CCNGG 1 cut(s) 232
BstV1I GCAGC 2 cut(s) 34, 127
BstX2I RGATCY 1 cut(s) 69
BstYI RGATCY 1 cut(s) 69
Bsu36I CCTNAGG 1 cut(s) 194
BsuRI GGCC 1 cut(s) 25
BtsIMutI CAGTG 1 cut(s) 38
Cac8I GCNNGC 1 cut(s) 17
Cfr13I GGNCC 1 cut(s) 24
Csp6I GTAC 1 cut(s) 178
CviJI RGCY 4 cut(s) 5, 19, 25, 140
CviKI_1 RGCY 4 cut(s) 5, 19, 25, 140
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 2 cut(s) 20, 194
DpnI GATC 1 cut(s) 71
DpnII GATC 1 cut(s) 69
EciI GGCGGA 1 cut(s) 80
Eco130I CCWWGG 1 cut(s) 126
Eco57I CTGAAG 1 cut(s) 132
Eco81I CCTNAGG 1 cut(s) 194
Eco88I CYCGRG 2 cut(s) 26, 259
EcoRII CCWGG 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 126
FaiI YATR 2 cut(s) 96, 230
FauI CCCGC 1 cut(s) 68
Fnu4HI GCNGC 3 cut(s) 6, 48, 141
Fsp4HI GCNGC 3 cut(s) 6, 48, 141
GluI GCNGC 3 cut(s) 6, 48, 141
GsuI CTGGAG 1 cut(s) 165
HaeIII GGCC 1 cut(s) 25
HapII CCGG 1 cut(s) 221
HinfI GANTC 2 cut(s) 10, 82
HpaII CCGG 1 cut(s) 221
HphI GGTGA 2 cut(s) 91, 244
Hpy188I TCNGA 1 cut(s) 119
Hpy188III TCNNGA 1 cut(s) 193
HpyCH4III ACNGT 1 cut(s) 42
HpyF3I CTNAG 2 cut(s) 20, 194
Kzo9I GATC 1 cut(s) 69
LmnI GCTCC 1 cut(s) 184
LpnPI CCDG 7 cut(s) 39, 123, 129, 206, 219, 234, 246
Lsp1109I GCAGC 2 cut(s) 34, 127
LweI GCATC 1 cut(s) 246
MaeIII GTNAC 1 cut(s) 121
MalI GATC 1 cut(s) 71
MboI GATC 1 cut(s) 69
MboII GAAGA 1 cut(s) 241
MflI RGATCY 1 cut(s) 69
MluCI AATT 2 cut(s) 200, 225
MlyI GAGTC 1 cut(s) 91
MnlI CCTC 4 cut(s) 15, 22, 160, 189
MspI CCGG 1 cut(s) 221
MspR9I CCNGG 1 cut(s) 234
MvaI CCWGG 1 cut(s) 234
NdeII GATC 1 cut(s) 69
NlaIV GGNNCC 1 cut(s) 219
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 1 cut(s) 121
PfeI GAWTC 1 cut(s) 10
PkrI GCNGC 3 cut(s) 7, 49, 142
PleI GAGTC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 232
PspGI CCWGG 1 cut(s) 232
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 1 cut(s) 24
PsuI RGATCY 1 cut(s) 69
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
SatI GCNGC 3 cut(s) 6, 48, 141
Sau3AI GATC 1 cut(s) 69
Sau96I GGNCC 1 cut(s) 24
SchI GAGTC 1 cut(s) 91
ScrFI CCNGG 1 cut(s) 234
SetI ASST 2 cut(s) 33, 142
SfaNI GCATC 1 cut(s) 246
Sse9I AATT 2 cut(s) 200, 225
SsiI CCGC 3 cut(s) 75, 91, 208
StyD4I CCNGG 1 cut(s) 232
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 1 cut(s) 42
TasI AATT 2 cut(s) 200, 225
TatI WGTACW 1 cut(s) 177
TfiI GAWTC 1 cut(s) 10
TscAI CASTG 1 cut(s) 45
TseFI GTSAC 1 cut(s) 121
TseI GCWGC 3 cut(s) 5, 47, 140
Tsp45I GTSAC 1 cut(s) 121
TspRI CASTG 1 cut(s) 45
XapI RAATTY 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.