MD13G1111000.v1.1

NADH-ubiquinone reductase complex 1 MLRQ subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004480
Physical Location & Seq
Reverse (-)
8057475 .. 8058215
741 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1111000.v1.1.491

Sequence Viewer

Length: 261 bp
ATGGATAAGGCCCGAGGTGTTCCCCCTATTGCTGCAGTTGGTATAGCTGTTGGCGTTTGCGGCATGCAGCTTGTTAGGAACATCACCACCAACCCTGAAGTCAGGGTTACCAAGCAGAACAGAATTGCAGGGGTGCTAGAGAACCATGCAGAGGGTGAGAGATACGCAGAACATGGACTCCGGAAATATGTCCGAAGCAGAGCCCCCCAGATCATGCCATCTATCAACAAATACTTCTCAGATCCAAAGATTCCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B12D

Protein Analysis

87

Amino Acids

9.51

Weight (kDa)

10.09

Isoelectric Point (pI)

38.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B12D PF06522 8 - 55 3.6e-15 NADH-ubiquinone reductase complex 1 MLRQ subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 180
AciI CCGC 1 cut(s) 60
AclWI GGATC 1 cut(s) 236
AcuI CTGAAG 1 cut(s) 117
AfiI CCNNNNNNNGG 1 cut(s) 151
AloI GAACNNNNNNTCC 2 cut(s) 162, 194
AluBI AGCT 2 cut(s) 47, 70
AluI AGCT 2 cut(s) 47, 70
AlwI GGATC 1 cut(s) 236
Ama87I CYCGRG 1 cut(s) 12
Aor13HI TCCGGA 1 cut(s) 180
AoxI GGCC 1 cut(s) 9
ApeKI GCWGC 2 cut(s) 32, 67
AspS9I GGNCC 1 cut(s) 10
AsuHPI GGTGA 2 cut(s) 76, 167
AvaI CYCGRG 1 cut(s) 12
BanII GRGCYC 1 cut(s) 205
BbvI GCAGC 2 cut(s) 19, 79
BccI CCATC 1 cut(s) 226
BfaI CTAG 1 cut(s) 137
BfmI CTRYAG 1 cut(s) 33
BisI GCNGC 3 cut(s) 33, 61, 68
BlsI GCNGC 3 cut(s) 34, 62, 69
BmeT110I CYCGRG 1 cut(s) 12
BmgT120I GGNCC 1 cut(s) 10
BsaJI CCNNGG 1 cut(s) 13
BsaWI WCCGGW 1 cut(s) 180
BsaXI ACNNNNNCTCC 2 cut(s) 162, 192
Bsc4I CCNNNNNNNGG 1 cut(s) 151
BseAI TCCGGA 1 cut(s) 180
BseDI CCNNGG 1 cut(s) 13
BseLI CCNNNNNNNGG 1 cut(s) 151
BseMII CTCAG 1 cut(s) 252
BseXI GCAGC 2 cut(s) 19, 79
BshFI GGCC 1 cut(s) 11
BsiHKCI CYCGRG 1 cut(s) 12
BsiSI CCGG 1 cut(s) 181
BslI CCNNNNNNNGG 1 cut(s) 151
BsnI GGCC 1 cut(s) 11
BsoBI CYCGRG 1 cut(s) 12
Bsp1286I GDGCHC 1 cut(s) 205
Bsp13I TCCGGA 1 cut(s) 180
Bsp143I GATC 2 cut(s) 210, 241
BspACI CCGC 1 cut(s) 60
BspANI GGCC 1 cut(s) 11
BspCNI CTCAG 1 cut(s) 251
BspEI TCCGGA 1 cut(s) 180
BspMAI CTGCAG 1 cut(s) 37
BspPI GGATC 1 cut(s) 236
BssECI CCNNGG 1 cut(s) 13
BssMI GATC 2 cut(s) 210, 241
BstC8I GCNNGC 1 cut(s) 65
BstDEI CTNAG 1 cut(s) 238
BstEII GGTNACC 1 cut(s) 106
BstKTI GATC 2 cut(s) 213, 244
BstMBI GATC 2 cut(s) 210, 241
BstMWI GCNNNNNNNGC 1 cut(s) 60
BstNSI RCATGY 1 cut(s) 67
BstPI GGTNACC 1 cut(s) 106
BstSFI CTRYAG 1 cut(s) 33
BstV1I GCAGC 2 cut(s) 19, 79
BstX2I RGATCY 1 cut(s) 241
BstYI RGATCY 1 cut(s) 241
BsuRI GGCC 1 cut(s) 11
Cac8I GCNNGC 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 10
CviAII CATG 4 cut(s) 64, 146, 173, 214
CviJI RGCY 4 cut(s) 11, 47, 70, 203
CviKI_1 RGCY 4 cut(s) 11, 47, 70, 203
DdeI CTNAG 1 cut(s) 238
DpnI GATC 2 cut(s) 212, 243
DpnII GATC 2 cut(s) 210, 241
Eco24I GRGCYC 1 cut(s) 205
Eco57I CTGAAG 1 cut(s) 117
Eco88I CYCGRG 1 cut(s) 12
Eco91I GGTNACC 1 cut(s) 106
EcoO65I GGTNACC 1 cut(s) 106
EcoT38I GRGCYC 1 cut(s) 205
FaeI CATG 4 cut(s) 67, 149, 176, 217
FaiI YATR 6 cut(s) 44, 65, 147, 174, 189, 215
FatI CATG 4 cut(s) 63, 145, 172, 213
Fnu4HI GCNGC 3 cut(s) 33, 61, 68
FriOI GRGCYC 1 cut(s) 205
Fsp4HI GCNGC 3 cut(s) 33, 61, 68
FspBI CTAG 1 cut(s) 137
GluI GCNGC 3 cut(s) 33, 61, 68
HaeIII GGCC 1 cut(s) 11
HapII CCGG 1 cut(s) 181
Hin1II CATG 4 cut(s) 67, 149, 176, 217
HinfI GANTC 2 cut(s) 177, 250
HpaII CCGG 1 cut(s) 181
HphI GGTGA 2 cut(s) 76, 167
Hpy188I TCNGA 2 cut(s) 194, 241
Hpy188III TCNNGA 1 cut(s) 181
HpyCH4V TGCA 4 cut(s) 35, 67, 128, 149
HpyF10VI GCNNNNNNNGC 1 cut(s) 60
HpyF3I CTNAG 1 cut(s) 238
Hsp92II CATG 4 cut(s) 67, 149, 176, 217
Kpn2I TCCGGA 1 cut(s) 180
Kzo9I GATC 2 cut(s) 210, 241
LpnPI CCDG 5 cut(s) 88, 108, 114, 194, 221
Lsp1109I GCAGC 2 cut(s) 19, 79
MaeI CTAG 1 cut(s) 137
MaeIII GTNAC 1 cut(s) 106
MalI GATC 2 cut(s) 212, 243
MboI GATC 2 cut(s) 210, 241
MflI RGATCY 1 cut(s) 241
MhlI GDGCHC 1 cut(s) 205
MluCI AATT 1 cut(s) 123
MlyI GAGTC 1 cut(s) 171
MnlI CCTC 2 cut(s) 8, 145
MroI TCCGGA 1 cut(s) 180
MspI CCGG 1 cut(s) 181
MwoI GCNNNNNNNGC 1 cut(s) 60
NdeII GATC 2 cut(s) 210, 241
NlaIII CATG 4 cut(s) 67, 149, 176, 217
NspI RCATGY 1 cut(s) 67
PaeI GCATGC 1 cut(s) 67
PfeI GAWTC 1 cut(s) 250
PkrI GCNGC 3 cut(s) 34, 62, 69
PleI GAGTC 1 cut(s) 171
PpsI GAGTC 1 cut(s) 171
PspEI GGTNACC 1 cut(s) 106
PspPI GGNCC 1 cut(s) 10
PstI CTGCAG 1 cut(s) 37
PsuI RGATCY 1 cut(s) 241
SatI GCNGC 3 cut(s) 33, 61, 68
Sau3AI GATC 2 cut(s) 210, 241
Sau96I GGNCC 1 cut(s) 10
SchI GAGTC 1 cut(s) 171
SduI GDGCHC 1 cut(s) 205
SetI ASST 3 cut(s) 19, 49, 72
SfcI CTRYAG 1 cut(s) 33
SphI GCATGC 1 cut(s) 67
Sse9I AATT 1 cut(s) 123
SsiI CCGC 1 cut(s) 60
SspMI CTAG 1 cut(s) 137
TasI AATT 1 cut(s) 123
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 1 cut(s) 250
TseI GCWGC 2 cut(s) 32, 67
XceI RCATGY 1 cut(s) 67
XspI CTAG 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.