MD16G1111000.v1.1

NADH-ubiquinone reductase complex 1 MLRQ subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004480
Physical Location & Seq
Reverse (-)
7795679 .. 7796530
852 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1111000.v1.1.491

Sequence Viewer

Length: 279 bp
ATGGCTTCCTCCTCCAGATGGATAAGGCCTGAGGTGTTCCCCCTATTTGCTGCAGTTGGTGTAGCTGTTGGCATTTGCGGCATGCAGCTTGTTAGGAACATCACCAGCAACCCTGAAGTCAGGGTTACCAAGCAGAACAGAACTGCAGGGGTGCTAGAGAATCATGAAGAGGGTGAGAGATACGCAGAACACGGACTCCGGAAATATGTCCGTACCCGAGCTCCCCAGATCATGCCATCCATCAACAAATACTTCTCCGATCCACAGATGCCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B12D

Protein Analysis

93

Amino Acids

10.41

Weight (kDa)

9.89

Isoelectric Point (pI)

60.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B12D PF06522 7 - 61 1.2e-22 NADH-ubiquinone reductase complex 1 MLRQ subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 198
AciI CCGC 1 cut(s) 78
AclWI GGATC 1 cut(s) 254
AcuI CTGAAG 1 cut(s) 135
AfaI GTAC 1 cut(s) 214
AfiI CCNNNNNNNGG 1 cut(s) 18
AloI GAACNNNNNNTCC 2 cut(s) 180, 212
AluBI AGCT 3 cut(s) 65, 88, 221
AluI AGCT 3 cut(s) 65, 88, 221
Alw21I GWGCWC 1 cut(s) 223
AlwI GGATC 1 cut(s) 254
Ama87I CYCGRG 1 cut(s) 216
Aor13HI TCCGGA 1 cut(s) 198
AoxI GGCC 1 cut(s) 26
ApeKI GCWGC 2 cut(s) 50, 85
AsuHPI GGTGA 2 cut(s) 94, 185
AvaI CYCGRG 1 cut(s) 216
AxyI CCTNAGG 1 cut(s) 30
BanII GRGCYC 1 cut(s) 223
Bbv12I GWGCWC 1 cut(s) 223
BbvI GCAGC 2 cut(s) 37, 97
BccI CCATC 3 cut(s) 12, 244, 248
BfaI CTAG 1 cut(s) 155
BfmI CTRYAG 2 cut(s) 51, 144
BisI GCNGC 3 cut(s) 51, 79, 86
BlsI GCNGC 3 cut(s) 52, 80, 87
BmeT110I CYCGRG 1 cut(s) 216
BmsI GCATC 1 cut(s) 258
BsaWI WCCGGW 1 cut(s) 198
BsaXI ACNNNNNCTCC 4 cut(s) 180, 205, 210, 235
Bsc4I CCNNNNNNNGG 1 cut(s) 18
Bse21I CCTNAGG 1 cut(s) 30
BseAI TCCGGA 1 cut(s) 198
BseGI GGATG 1 cut(s) 236
BseLI CCNNNNNNNGG 1 cut(s) 18
BseMII CTCAG 1 cut(s) 21
BseXI GCAGC 2 cut(s) 37, 97
BshFI GGCC 1 cut(s) 28
BsiHKAI GWGCWC 1 cut(s) 223
BsiHKCI CYCGRG 1 cut(s) 216
BsiSI CCGG 1 cut(s) 199
BslI CCNNNNNNNGG 1 cut(s) 18
BsnI GGCC 1 cut(s) 28
BsoBI CYCGRG 1 cut(s) 216
Bsp1286I GDGCHC 1 cut(s) 223
Bsp13I TCCGGA 1 cut(s) 198
Bsp143I GATC 2 cut(s) 228, 259
BspACI CCGC 1 cut(s) 78
BspANI GGCC 1 cut(s) 28
BspCNI CTCAG 1 cut(s) 22
BspEI TCCGGA 1 cut(s) 198
BspHI TCATGA 1 cut(s) 163
BspMAI CTGCAG 2 cut(s) 55, 148
BspPI GGATC 1 cut(s) 254
BssMI GATC 2 cut(s) 228, 259
Bst6I CTCTTC 1 cut(s) 162
BstC8I GCNNGC 1 cut(s) 83
BstDEI CTNAG 1 cut(s) 30
BstEII GGTNACC 1 cut(s) 124
BstF5I GGATG 1 cut(s) 236
BstKTI GATC 2 cut(s) 231, 262
BstMBI GATC 2 cut(s) 228, 259
BstMWI GCNNNNNNNGC 1 cut(s) 78
BstNSI RCATGY 1 cut(s) 85
BstPI GGTNACC 1 cut(s) 124
BstSFI CTRYAG 2 cut(s) 51, 144
BstV1I GCAGC 2 cut(s) 37, 97
Bsu36I CCTNAGG 1 cut(s) 30
BsuRI GGCC 1 cut(s) 28
BtsCI GGATG 1 cut(s) 236
Cac8I GCNNGC 1 cut(s) 83
CciI TCATGA 1 cut(s) 163
Csp6I GTAC 1 cut(s) 213
CviAII CATG 3 cut(s) 82, 164, 232
CviJI RGCY 5 cut(s) 5, 28, 65, 88, 221
CviKI_1 RGCY 5 cut(s) 5, 28, 65, 88, 221
CviQI GTAC 1 cut(s) 213
DdeI CTNAG 1 cut(s) 30
DpnI GATC 2 cut(s) 230, 261
DpnII GATC 2 cut(s) 228, 259
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
Ecl136II GAGCTC 1 cut(s) 221
Eco147I AGGCCT 1 cut(s) 28
Eco24I GRGCYC 1 cut(s) 223
Eco53kI GAGCTC 1 cut(s) 221
Eco57I CTGAAG 1 cut(s) 135
Eco81I CCTNAGG 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 216
Eco91I GGTNACC 1 cut(s) 124
EcoICRI GAGCTC 1 cut(s) 221
EcoO65I GGTNACC 1 cut(s) 124
EcoT38I GRGCYC 1 cut(s) 223
FaeI CATG 3 cut(s) 85, 167, 235
FaiI YATR 4 cut(s) 83, 165, 207, 233
FatI CATG 3 cut(s) 81, 163, 231
Fnu4HI GCNGC 3 cut(s) 51, 79, 86
FokI GGATG 1 cut(s) 223
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 3 cut(s) 51, 79, 86
FspBI CTAG 1 cut(s) 155
GluI GCNGC 3 cut(s) 51, 79, 86
HaeIII GGCC 1 cut(s) 28
HapII CCGG 1 cut(s) 199
Hin1II CATG 3 cut(s) 85, 167, 235
HinfI GANTC 2 cut(s) 160, 195
HpaII CCGG 1 cut(s) 199
HphI GGTGA 2 cut(s) 94, 185
Hpy188I TCNGA 1 cut(s) 259
Hpy188III TCNNGA 3 cut(s) 15, 164, 199
HpyCH4V TGCA 3 cut(s) 53, 85, 146
HpyF10VI GCNNNNNNNGC 1 cut(s) 78
HpyF3I CTNAG 1 cut(s) 30
Hsp92II CATG 3 cut(s) 85, 167, 235
Kpn2I TCCGGA 1 cut(s) 198
Kzo9I GATC 2 cut(s) 228, 259
LmnI GCTCC 1 cut(s) 226
LpnPI CCDG 8 cut(s) 28, 42, 106, 118, 126, 132, 212, 239
Lsp1109I GCAGC 2 cut(s) 37, 97
LweI GCATC 1 cut(s) 258
MaeI CTAG 1 cut(s) 155
MaeIII GTNAC 1 cut(s) 124
MalI GATC 2 cut(s) 230, 261
MboI GATC 2 cut(s) 228, 259
MboII GAAGA 1 cut(s) 179
MhlI GDGCHC 1 cut(s) 223
MlyI GAGTC 1 cut(s) 189
MnlI CCTC 4 cut(s) 19, 22, 25, 163
MroI TCCGGA 1 cut(s) 198
MspI CCGG 1 cut(s) 199
MwoI GCNNNNNNNGC 1 cut(s) 78
NdeII GATC 2 cut(s) 228, 259
NlaIII CATG 3 cut(s) 85, 167, 235
NspI RCATGY 1 cut(s) 85
PaeI GCATGC 1 cut(s) 85
PagI TCATGA 1 cut(s) 163
PceI AGGCCT 1 cut(s) 28
PfeI GAWTC 1 cut(s) 160
PkrI GCNGC 3 cut(s) 52, 80, 87
PleI GAGTC 1 cut(s) 189
PpsI GAGTC 1 cut(s) 189
Psp124BI GAGCTC 1 cut(s) 223
PspEI GGTNACC 1 cut(s) 124
PstI CTGCAG 2 cut(s) 55, 148
RsaI GTAC 1 cut(s) 214
RsaNI GTAC 1 cut(s) 213
SacI GAGCTC 1 cut(s) 223
SatI GCNGC 3 cut(s) 51, 79, 86
Sau3AI GATC 2 cut(s) 228, 259
SchI GAGTC 1 cut(s) 189
SduI GDGCHC 1 cut(s) 223
SetI ASST 4 cut(s) 36, 67, 90, 223
SfaNI GCATC 1 cut(s) 258
SfcI CTRYAG 2 cut(s) 51, 144
SphI GCATGC 1 cut(s) 85
SseBI AGGCCT 1 cut(s) 28
SsiI CCGC 1 cut(s) 78
SspMI CTAG 1 cut(s) 155
SstI GAGCTC 1 cut(s) 223
StuI AGGCCT 1 cut(s) 28
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 1 cut(s) 160
TseI GCWGC 2 cut(s) 50, 85
TspDTI ATGAA 1 cut(s) 180
TspGWI ACGGA 2 cut(s) 200, 207
XceI RCATGY 1 cut(s) 85
XspI CTAG 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.