MD13G1111400.v1.1

NADH-ubiquinone reductase complex 1 MLRQ subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004480
Physical Location & Seq
Reverse (-)
8111575 .. 8112340
766 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1111400.v1.1.491

Sequence Viewer

Length: 279 bp
ATGGCTTCCTCCTCCAGATGGATAAGGCCCGAGGTGTTCCCCCTATTTGCTGCAGTTGGTATAGCTGTTGGCATTTGCGGCATGCAGCTTGTTAGGAACATCACCACCAACCCTGAAGTCAGGGTTACCAAGCAGAACAGAATTGCAGGGGTGCTAGAGAACCATGCAGAGGGTGAGAGATACGCAGAACATGGACTCCGGAAATATGTCCGAAGCAGAGCCCCCCAGATCATGCCATCTATCAACAAATACTTCTCAGATCCAAAGATTCCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B12D

Protein Analysis

93

Amino Acids

10.36

Weight (kDa)

10.2

Isoelectric Point (pI)

53.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B12D PF06522 7 - 61 4.3e-22 NADH-ubiquinone reductase complex 1 MLRQ subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 198
AciI CCGC 1 cut(s) 78
AclWI GGATC 1 cut(s) 254
AcuI CTGAAG 1 cut(s) 135
AfiI CCNNNNNNNGG 2 cut(s) 18, 169
AloI GAACNNNNNNTCC 2 cut(s) 180, 212
AluBI AGCT 2 cut(s) 65, 88
AluI AGCT 2 cut(s) 65, 88
AlwI GGATC 1 cut(s) 254
Ama87I CYCGRG 1 cut(s) 29
Aor13HI TCCGGA 1 cut(s) 198
AoxI GGCC 1 cut(s) 26
ApeKI GCWGC 2 cut(s) 50, 85
AspS9I GGNCC 1 cut(s) 27
AsuHPI GGTGA 2 cut(s) 94, 185
AvaI CYCGRG 1 cut(s) 29
BanII GRGCYC 1 cut(s) 223
BbvI GCAGC 2 cut(s) 37, 97
BccI CCATC 2 cut(s) 12, 244
BfaI CTAG 1 cut(s) 155
BfmI CTRYAG 1 cut(s) 51
BisI GCNGC 3 cut(s) 51, 79, 86
BlsI GCNGC 3 cut(s) 52, 80, 87
BmeT110I CYCGRG 1 cut(s) 29
BmgT120I GGNCC 1 cut(s) 27
BsaJI CCNNGG 1 cut(s) 30
BsaWI WCCGGW 1 cut(s) 198
BsaXI ACNNNNNCTCC 2 cut(s) 180, 210
Bsc4I CCNNNNNNNGG 2 cut(s) 18, 169
BseAI TCCGGA 1 cut(s) 198
BseDI CCNNGG 1 cut(s) 30
BseLI CCNNNNNNNGG 2 cut(s) 18, 169
BseMII CTCAG 1 cut(s) 270
BseXI GCAGC 2 cut(s) 37, 97
BshFI GGCC 1 cut(s) 28
BsiHKCI CYCGRG 1 cut(s) 29
BsiSI CCGG 1 cut(s) 199
BslI CCNNNNNNNGG 2 cut(s) 18, 169
BsnI GGCC 1 cut(s) 28
BsoBI CYCGRG 1 cut(s) 29
Bsp1286I GDGCHC 1 cut(s) 223
Bsp13I TCCGGA 1 cut(s) 198
Bsp143I GATC 2 cut(s) 228, 259
BspACI CCGC 1 cut(s) 78
BspANI GGCC 1 cut(s) 28
BspCNI CTCAG 1 cut(s) 269
BspEI TCCGGA 1 cut(s) 198
BspMAI CTGCAG 1 cut(s) 55
BspPI GGATC 1 cut(s) 254
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 2 cut(s) 228, 259
BstC8I GCNNGC 1 cut(s) 83
BstDEI CTNAG 1 cut(s) 256
BstEII GGTNACC 1 cut(s) 124
BstKTI GATC 2 cut(s) 231, 262
BstMBI GATC 2 cut(s) 228, 259
BstMWI GCNNNNNNNGC 1 cut(s) 78
BstNSI RCATGY 1 cut(s) 85
BstPI GGTNACC 1 cut(s) 124
BstSFI CTRYAG 1 cut(s) 51
BstV1I GCAGC 2 cut(s) 37, 97
BstX2I RGATCY 1 cut(s) 259
BstYI RGATCY 1 cut(s) 259
BsuRI GGCC 1 cut(s) 28
Cac8I GCNNGC 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 27
CviAII CATG 4 cut(s) 82, 164, 191, 232
CviJI RGCY 5 cut(s) 5, 28, 65, 88, 221
CviKI_1 RGCY 5 cut(s) 5, 28, 65, 88, 221
DdeI CTNAG 1 cut(s) 256
DpnI GATC 2 cut(s) 230, 261
DpnII GATC 2 cut(s) 228, 259
Eco24I GRGCYC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 135
Eco88I CYCGRG 1 cut(s) 29
Eco91I GGTNACC 1 cut(s) 124
EcoO65I GGTNACC 1 cut(s) 124
EcoT38I GRGCYC 1 cut(s) 223
FaeI CATG 4 cut(s) 85, 167, 194, 235
FaiI YATR 6 cut(s) 62, 83, 165, 192, 207, 233
FatI CATG 4 cut(s) 81, 163, 190, 231
Fnu4HI GCNGC 3 cut(s) 51, 79, 86
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 3 cut(s) 51, 79, 86
FspBI CTAG 1 cut(s) 155
GluI GCNGC 3 cut(s) 51, 79, 86
HaeIII GGCC 1 cut(s) 28
HapII CCGG 1 cut(s) 199
Hin1II CATG 4 cut(s) 85, 167, 194, 235
HinfI GANTC 2 cut(s) 195, 268
HpaII CCGG 1 cut(s) 199
HphI GGTGA 2 cut(s) 94, 185
Hpy188I TCNGA 2 cut(s) 212, 259
Hpy188III TCNNGA 2 cut(s) 15, 199
HpyCH4V TGCA 4 cut(s) 53, 85, 146, 167
HpyF10VI GCNNNNNNNGC 1 cut(s) 78
HpyF3I CTNAG 1 cut(s) 256
Hsp92II CATG 4 cut(s) 85, 167, 194, 235
Kpn2I TCCGGA 1 cut(s) 198
Kzo9I GATC 2 cut(s) 228, 259
LpnPI CCDG 6 cut(s) 28, 106, 126, 132, 212, 239
Lsp1109I GCAGC 2 cut(s) 37, 97
MaeI CTAG 1 cut(s) 155
MaeIII GTNAC 1 cut(s) 124
MalI GATC 2 cut(s) 230, 261
MboI GATC 2 cut(s) 228, 259
MflI RGATCY 1 cut(s) 259
MhlI GDGCHC 1 cut(s) 223
MluCI AATT 1 cut(s) 141
MlyI GAGTC 1 cut(s) 189
MnlI CCTC 4 cut(s) 19, 22, 25, 163
MroI TCCGGA 1 cut(s) 198
MspI CCGG 1 cut(s) 199
MwoI GCNNNNNNNGC 1 cut(s) 78
NdeII GATC 2 cut(s) 228, 259
NlaIII CATG 4 cut(s) 85, 167, 194, 235
NspI RCATGY 1 cut(s) 85
PaeI GCATGC 1 cut(s) 85
PfeI GAWTC 1 cut(s) 268
PkrI GCNGC 3 cut(s) 52, 80, 87
PleI GAGTC 1 cut(s) 189
PpsI GAGTC 1 cut(s) 189
PspEI GGTNACC 1 cut(s) 124
PspPI GGNCC 1 cut(s) 27
PstI CTGCAG 1 cut(s) 55
PsuI RGATCY 1 cut(s) 259
SatI GCNGC 3 cut(s) 51, 79, 86
Sau3AI GATC 2 cut(s) 228, 259
Sau96I GGNCC 1 cut(s) 27
SchI GAGTC 1 cut(s) 189
SduI GDGCHC 1 cut(s) 223
SetI ASST 3 cut(s) 36, 67, 90
SfcI CTRYAG 1 cut(s) 51
SphI GCATGC 1 cut(s) 85
Sse9I AATT 1 cut(s) 141
SsiI CCGC 1 cut(s) 78
SspMI CTAG 1 cut(s) 155
TasI AATT 1 cut(s) 141
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 1 cut(s) 268
TseI GCWGC 2 cut(s) 50, 85
XceI RCATGY 1 cut(s) 85
XspI CTAG 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.