Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0002043

Physical Location & Seq

Genomic Coordinates Reverse (-)

27135494 .. 27140404

Gene Structure 4911 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g25290.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2520 bp

ATGGCCCTTCAAGCTCAAAACCCCCTTACTCCCCAGAATAGGGTCAACTTTCAAAGCAACCTTGCACCTCAGAATAGGGTCACTTTTCAAGACAACCTTGCACCTCAGAATGCTGGCCTTGCACCTCAAACTGCCGGCCTTGTGCCTCAGCAAAATCCCAGCCTTGTGCCTCAAGGTACTGCCCCTGTACCTCAAAAGACCTTTGCACCTCAGAGTGCACTTGCACCTCAAAGTGCCTATGCACCTCAAACACTTGCAGCTCAAAGTGCCCTTGTGCATCCAAATAAGCCGCCCGGCACTGTCCTAGGCCGAGGAGGAGACAACTACCGGGACAACTACCGTACTGCTGGTCCTACTCAGCTGGGTAGAGATCGTCGTCAGTATTCAGCACCATCGGATGACAATACCTTAAGCAACCAAATTCTGATCACTGATAGATCGCATGAACGTCCCTACGATGTGCTTCCTCTTTCTGTCAAGCAGGTTCTCCAAACAATCGAGGCCATTCTGGTTCGTGCCAACAAACCAGTTGACATTCATGGCACTGTAGTTCCGGGAGTTCTTGTACCTGCAACTGCACAAGTGGATAGTCTGGAACATGAGAGGGCCATCCATGCCAGCCTGAGTGCCCTCCACGACAACCTCGACGTTCCAAACAGCGTTCTGAATGAAATTTCCTGCGAGATACAATGTAAGTCGCTATCAGGAGTAGACGCAAACAAGACCACCATGGACATTTTAGAGAGGGTACAGCACTACGATTGGGATGAAAAGGTGGTGCTTGCCTTGGCATCTTTTGCAATCAAAGATGGTGAATTTTGGCTTGTGGCTCAGCTTTTCACCAGCAACCCACTGGCCAAAGCCATTGGACAACTGAAGCAAATGCAAGAGATACTAGAACGATCAGGGACTAGTTTGAAAGCAAAGTTTGAGGTGTACAACAATCTGGTTAATGCCGTGCTGAAGGTGACCCGAATCATTGTTCACTTGCAGGACATTTACCACGACCCACACTTAGCCACTGAAATCAAAGCCACAACTCCCACTAATCATATCCCCAGTGCTGTTTACTGGACTATCCGGAGTATAGTCATTCTTGCATCACAGCTTTTAGGGATCACCGGCTCTGATCCGGAGTACGTAACAGAGGCATGGGAACTATCATCCTTGGCACACAAGCTCGAAAGCATATACAAACATCTTGAGGATAACCTAAACAGACTCCATGAACTCACTCGGAAGTACAGAGATGAAGAAGCATTCGCTGCAATTGAACGCATCTTAGAGTCACCACATATTGATAACAGCAAGCCATTGAGGGTTTTGTTTTACAAGGATGACCAACCTGCACTCTTTGATGGCTACAACAAGAAGAGGGTGGACATTGATGTGCTGAGGAGGAAGGTGGTGATACTGATGATTTCAGACCTGGACATCGGCCAGGAAAATGAGTACATAATTGCTCATCAAATGTACTCGGAGAAACGCCAGTTCCCAACAAGGGCGGAGAGCCAGTATGAGATTGTCTGGGTTCCAATTGTGGACAACTGGACTGAAGCTAAGCTGCTTCAGTTTGAGGAGATTAGAAATGGCATGGAATGGTACAGTGTGTACCACCCTTCGTTTGTGTCTCCGATTGTGGTGAAGTACATTAAGGACAAGAGGAAATGGAACTTTGTGAAGAAGCCTCTGCTTGTGGTGATGGACCCTCAAGGCAAAATAGTGCACACCAATGCTGTTCACATGATGTGTGTTTTCGGAAGCGCAGCATACCCATTCACAAGCAACAGAGAGAGATTGCTCTGGGAAGAAGAGACCTGGAGGATTGAGCTTTTGGCAGATGCTATTGATCAAACCTTACTTAACTGGATTGCAGAAAGGAAGTACATTTGCTTGTATGGAGGGGAAGACATTAACTGGATCCGAAACTTCACAAGGACTGCAAAAAATGTGGCCTTGGAAGCTGGTATCCAGTTAGAGTTGCTCTATCTTGGCAAAAGCAAGCAGAAGGAGCGAGTGGTCAAAGACATCATCCGCGTCATTAAGGAGGAGAAGCTGAGCCACACCATCGACTCGAACCTCATTTGGTTCTTCTGGGTTCGTCTTGAGAGCATGTGGCAGTCCAAAGGACAGCTTATGAGTGAGCTCTCCAAGGCTAACTTCACAGACCATAACATAAAAAACGACATCATAATGCAGGGGATCGTGTCCCTGCTGAGCTTCGGTTCTACGGACCGAGGGTGGGCACTGATCGCGCTTCCTTCATCAGAAATGGCTAAAGCTAATGGGGAGCACATGTCAAAGAGCTTGAACGACTTCAAGATTTGGAAACCTAAGCACGATGAGGTAGGGTTTACACCTGCACTAAACGAATATCTTACTGGGGTATACAGGGACGCCCCACATCACTGCACCAATCTCATACTGCCTGCAACCGGCATCATGCCTGAGACTGTGTCCTGCTCAGAATGTGGTCGTATCATGGAGAGGTATGCCATGTTCCGCTGCTGCACTGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0008150 GO:0009888 GO:0010087 GO:0010088 GO:0032502 GO:0042802 GO:0042803 GO:0043621 GO:0046983 GO:0048856

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SEO_C SEO_N

Protein Analysis

840

Amino Acids

95.13

Weight (kDa)

6.29

Isoelectric Point (pI)

42.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SEO_N	PF14576	132 - 432	1.2e-67	Sieve element occlusion N-terminus
SEO_C	PF14577	597 - 838	2.5e-83	Sieve element occlusion C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002043)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g25290 FvH4_4g25290 FvH4_4g25290 FvH4_4g25310 FvH4_4g25320
malus_domestica	MD13G1105000.v1.1 MD13G1105100.v1.1 MD16G1104800.v1.1 MD16G1105000.v1.1
prunus_persica	Prupe.1G241400_v2.0.a1 Prupe.1G241500_v2.0.a1
pyrus_communis	pycom13g09110 pycom13g09120 pycom16g08950 pycom16g08960
rosa_chinensis	RchiOBHm_Chr4g0432561 RchiOBHm_Chr4g0432571 RchiOBHm_Chr4g0432611
rosa_laevigata	RLG00000006831 RLG00000006832 RLG00000006833
rosa_multiflora	Rmu_sc0003886.1_g000001 Rmu_sc0004612.1_g000014 Rmu_sc0027699.1_g000001
rosa_roxburghii	Rroxscaffold_5G00373990 Rroxscaffold_5G00374000 Rroxscaffold_5G00374030
rosa_rugosa	Rorug04G0260100 Rorug04G0260100 Rorug04G0260200
rosa_samantha	Rh4AG315100 Rh4AG315200 Rh4AG315300 Rh4BG322800 Rh4BG322900 Rh4BG323000 Rh4CG337900 Rh4CG338000 Rh4CG338100 Rh4DG318600 Rh4DG318700 Rh4DG318800
rosa_wichuraiana	Rw4G027380 Rw4G027390 Rw4G027420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2368
Acc36I	ACCTGC	4 cut(s)	472, 577, 1354, 2368
AccI	GTMKAC	2 cut(s)	711, 2388
AccII	CGCG	2 cut(s)	2037, 2255
AccIII	TCCGGA	2 cut(s)	1080, 1132
AciI	CCGC	4 cut(s)	290, 1505, 2035, 2503
AclWI	GGATC	5 cut(s)	1124, 1124, 1915, 1928, 2210
AcoI	YGGCCR	2 cut(s)	855, 1438
AcsI	RAATTY	3 cut(s)	420, 672, 815
AcuI	CTGAAG	4 cut(s)	896, 983, 1553, 1575
AcyI	GRCGYC	1 cut(s)	2397
AfiI	CCNNNNNNNGG	5 cut(s)	39, 40, 1501, 2242, 2435
AflII	CTTAAG	1 cut(s)	409
AflIII	ACRYGT	1 cut(s)	2295
AgsI	TTSAA	7 cut(s)	11, 53, 89, 919, 1274, 2311, 2320
AhlI	ACTAGT	1 cut(s)	911
AjnI	CCWGG	3 cut(s)	1428, 1440, 1817
AjuI	GAANNNNNNNTTGG	2 cut(s)	2144, 2176
Alw21I	GWGCWC	4 cut(s)	220, 1728, 2148, 2295
Alw26I	GTCTC	4 cut(s)	312, 1635, 1808, 2444
Alw44I	GTGCAC	2 cut(s)	216, 1724
AlwI	GGATC	5 cut(s)	1124, 1124, 1915, 1928, 2210
Aor13HI	TCCGGA	2 cut(s)	1080, 1132
AoxI	GGCC	9 cut(s)	3, 115, 136, 307, 501, 606, 855, 1438, 1953
ApaLI	GTGCAC	2 cut(s)	216, 1724
ApeKI	GCWGC	6 cut(s)	257, 1265, 1564, 1766, 2505, 2508
ApoI	RAATTY	3 cut(s)	420, 672, 815
Asp700I	GAANNNNTTC	1 cut(s)	2315
AspA2I	CCTAGG	1 cut(s)	304
AspLEI	GCGC	2 cut(s)	1766, 2257
AspS9I	GGNCC	5 cut(s)	4, 350, 606, 1705, 2233
AsuC2I	CCSGG	3 cut(s)	294, 329, 555
AsuHPI	GGTGA	8 cut(s)	824, 832, 979, 1111, 1281, 1420, 1654, 1711
AvaII	GGWCC	3 cut(s)	350, 1705, 2233
AvrII	CCTAGG	1 cut(s)	304
BaeGI	GKGCMC	5 cut(s)	220, 271, 631, 1728, 2248
BalI	TGGCCA	1 cut(s)	857
BamHI	GGATCC	1 cut(s)	1920
BanII	GRGCYC	1 cut(s)	2148
BbsI	GAAGAC	1 cut(s)	1914
Bbv12I	GWGCWC	4 cut(s)	220, 1728, 2148, 2295
BbvCI	CCTCAGC	2 cut(s)	147, 1394
BbvI	GCAGC	6 cut(s)	269, 1252, 1551, 1778, 2492, 2495
BccI	CCATC	6 cut(s)	400, 617, 803, 1352, 1696, 2075
BceAI	ACGGC	1 cut(s)	941
BciT130I	CCWGG	3 cut(s)	1430, 1442, 1819
BciVI	GTATCC	1 cut(s)	1979
BclI	TGATCA	2 cut(s)	426, 1849
BcnI	CCSGG	3 cut(s)	294, 329, 555
BcoDI	GTCTC	4 cut(s)	312, 1635, 1808, 2444
BcuI	ACTAGT	1 cut(s)	911
BfaI	CTAG	3 cut(s)	305, 896, 912
BfmI	CTRYAG	1 cut(s)	546
BfrI	CTTAAG	1 cut(s)	409
BfuAI	ACCTGC	4 cut(s)	472, 577, 1354, 2368
BfuI	GTATCC	1 cut(s)	1979
BisI	GCNGC	7 cut(s)	258, 290, 1266, 1565, 1767, 2506, 2509
BlnI	CCTAGG	1 cut(s)	304
BlpI	GCTNAGC	4 cut(s)	831, 1560, 2057, 2216
BlsI	GCNGC	7 cut(s)	259, 291, 1267, 1566, 1768, 2507, 2510
Bme1390I	CCNGG	6 cut(s)	294, 329, 555, 1430, 1442, 1819
Bme18I	GGWCC	3 cut(s)	350, 1705, 2233
BmgT120I	GGNCC	5 cut(s)	4, 350, 606, 1705, 2233
BmiI	GGNNCC	3 cut(s)	1533, 1707, 1922
BmrFI	CCNGG	6 cut(s)	294, 329, 555, 1430, 1442, 1819
BmrI	ACTGGG	2 cut(s)	1053, 2391
BmsI	GCATC	6 cut(s)	286, 800, 1109, 1287, 1831, 2448
BmuI	ACTGGG	2 cut(s)	1053, 2391
BpiI	GAAGAC	1 cut(s)	1914
BpmI	CTGGAG	1 cut(s)	1840
Bpu10I	CCTNAGC	3 cut(s)	147, 1394, 2334
Bpu1102I	GCTNAGC	4 cut(s)	831, 1560, 2057, 2216
BpuEI	CTTGAG	4 cut(s)	156, 1223, 1695, 2126
BpuMI	CCSGG	3 cut(s)	294, 329, 555
BsaAI	YACGTR	1 cut(s)	1141
BsaHI	GRCGYC	1 cut(s)	2397
BsaI	GGTCTC	1 cut(s)	1808
BsaJI	CCNNGG	8 cut(s)	304, 310, 729, 786, 1167, 1956, 2151, 2236
BsaWI	WCCGGW	2 cut(s)	1080, 1132
BsaXI	ACNNNNNCTCC	6 cut(s)	549, 579, 1499, 1529, 2282, 2312
Bsc4I	CCNNNNNNNGG	5 cut(s)	39, 40, 1501, 2242, 2435
Bse118I	RCCGGY	3 cut(s)	134, 1121, 2435
BseAI	TCCGGA	2 cut(s)	1080, 1132
BseBI	CCWGG	3 cut(s)	1430, 1442, 1819
BseDI	CCNNGG	8 cut(s)	304, 310, 729, 786, 1167, 1956, 2151, 2236
BseGI	GGATG	7 cut(s)	277, 403, 609, 772, 1163, 1342, 2031
BseLI	CCNNNNNNNGG	5 cut(s)	39, 40, 1501, 2242, 2435
BseRI	GAGGAG	5 cut(s)	327, 330, 1411, 1592, 2063
BseSI	GKGCMC	5 cut(s)	220, 271, 631, 1728, 2248
BseXI	GCAGC	6 cut(s)	269, 1252, 1551, 1778, 2492, 2495
BseYI	CCCAGC	2 cut(s)	158, 361
BsgI	GTGCAG	5 cut(s)	561, 1332, 2346, 2395, 2494
Bsh1236I	CGCG	2 cut(s)	2037, 2255
BshFI	GGCC	9 cut(s)	5, 117, 138, 309, 503, 608, 857, 1440, 1955
BsiHKAI	GWGCWC	4 cut(s)	220, 1728, 2148, 2295
BsiSI	CCGG	8 cut(s)	135, 294, 328, 554, 1081, 1122, 1133, 2436
BslFI	GGGAC	5 cut(s)	344, 435, 922, 2194, 2408
BslI	CCNNNNNNNGG	5 cut(s)	39, 40, 1501, 2242, 2435
BsmAI	GTCTC	4 cut(s)	312, 1635, 1808, 2444
BsmFI	GGGAC	5 cut(s)	344, 435, 922, 2194, 2408
BsmI	GAATGC	2 cut(s)	115, 1259
BsnI	GGCC	9 cut(s)	5, 117, 138, 309, 503, 608, 857, 1440, 1955
Bso31I	GGTCTC	1 cut(s)	1808
Bsp1286I	GDGCHC	7 cut(s)	220, 271, 631, 1728, 2148, 2248, 2295
Bsp13I	TCCGGA	2 cut(s)	1080, 1132
Bsp1407I	TGTACA	1 cut(s)	936
Bsp1720I	GCTNAGC	4 cut(s)	831, 1560, 2057, 2216
Bsp19I	CCATGG	1 cut(s)	729
BspACI	CCGC	4 cut(s)	290, 1505, 2035, 2503
BspANI	GGCC	9 cut(s)	5, 117, 138, 309, 503, 608, 857, 1440, 1955
BspEI	TCCGGA	2 cut(s)	1080, 1132
BspFNI	CGCG	2 cut(s)	2037, 2255
BspLI	GGNNCC	3 cut(s)	1533, 1707, 1922
BspMI	ACCTGC	4 cut(s)	472, 577, 1354, 2368
BspPI	GGATC	5 cut(s)	1124, 1124, 1915, 1928, 2210
BspTI	CTTAAG	1 cut(s)	409
BspTNI	GGTCTC	1 cut(s)	1808
BsrFI	RCCGGY	3 cut(s)	134, 1121, 2435
BsrGI	TGTACA	1 cut(s)	936
BssAI	RCCGGY	3 cut(s)	134, 1121, 2435
BssECI	CCNNGG	8 cut(s)	304, 310, 729, 786, 1167, 1956, 2151, 2236
BssNAI	GTATAC	1 cut(s)	2389
BssNI	GRCGYC	1 cut(s)	2397
BssT1I	CCWWGG	6 cut(s)	304, 729, 786, 1167, 1956, 2151
Bst1107I	GTATAC	1 cut(s)	2389
Bst2UI	CCWGG	3 cut(s)	1430, 1442, 1819
Bst4CI	ACNGT	5 cut(s)	301, 341, 547, 1607, 2455
Bst6I	CTCTTC	2 cut(s)	1367, 1806
BstACI	GRCGYC	1 cut(s)	2397
BstAFI	CTTAAG	1 cut(s)	409
BstAPI	GCANNNNNTGC	2 cut(s)	797, 1265
BstAUI	TGTACA	1 cut(s)	936
BstBAI	YACGTR	1 cut(s)	1141
BstC8I	GCNNGC	7 cut(s)	115, 136, 619, 783, 1310, 2003, 2430
BstDSI	CCRYGG	1 cut(s)	729
BstEII	GGTNACC	1 cut(s)	967
BstF5I	GGATG	7 cut(s)	277, 403, 609, 772, 1163, 1342, 2031
BstFNI	CGCG	2 cut(s)	2037, 2255
BstHHI	GCGC	2 cut(s)	1766, 2257
BstMAI	GTCTC	4 cut(s)	312, 1635, 1808, 2444
BstMWI	GCNNNNNNNGC	9 cut(s)	11, 119, 266, 614, 797, 1265, 1961, 2011, 2252
BstNI	CCWGG	3 cut(s)	1430, 1442, 1819
BstNSI	RCATGY	2 cut(s)	2116, 2299
BstPI	GGTNACC	1 cut(s)	967
BstSCI	CCNGG	6 cut(s)	292, 327, 553, 1428, 1440, 1817
BstSFI	CTRYAG	1 cut(s)	546
BstSLI	GKGCMC	5 cut(s)	220, 271, 631, 1728, 2248
BstSNI	TACGTA	1 cut(s)	1141
BstUI	CGCG	2 cut(s)	2037, 2255
BstV1I	GCAGC	6 cut(s)	269, 1252, 1551, 1778, 2492, 2495
BstV2I	GAAGAC	1 cut(s)	1914
BstX2I	RGATCY	1 cut(s)	1920
BstYI	RGATCY	1 cut(s)	1920
BstZ17I	GTATAC	1 cut(s)	2389
BsuI	GTATCC	1 cut(s)	1979
BsuRI	GGCC	9 cut(s)	5, 117, 138, 309, 503, 608, 857, 1440, 1955
BtgI	CCRYGG	1 cut(s)	729
BtsCI	GGATG	7 cut(s)	277, 403, 609, 772, 1163, 1342, 2031
BtsI	GCAGTG	1 cut(s)	2407
BveI	ACCTGC	4 cut(s)	472, 577, 1354, 2368
Cac8I	GCNNGC	7 cut(s)	115, 136, 619, 783, 1310, 2003, 2430
CfoI	GCGC	2 cut(s)	1766, 2257
Cfr10I	RCCGGY	3 cut(s)	134, 1121, 2435
Cfr13I	GGNCC	5 cut(s)	4, 350, 606, 1705, 2233
CpoI	CGGWCCG	1 cut(s)	2233
CseI	GACGC	3 cut(s)	722, 2026, 2405
CspCI	CAANNNNNGTGG	2 cut(s)	1932, 1967
CspI	CGGWCCG	1 cut(s)	2233
EaeI	YGGCCR	2 cut(s)	855, 1438
Eam1104I	CTCTTC	2 cut(s)	1367, 1806
EarI	CTCTTC	2 cut(s)	1367, 1806
EciI	GGCGGA	1 cut(s)	1520
Ecl136II	GAGCTC	1 cut(s)	2146
Eco105I	TACGTA	1 cut(s)	1141
Eco130I	CCWWGG	6 cut(s)	304, 729, 786, 1167, 1956, 2151
Eco24I	GRGCYC	1 cut(s)	2148
Eco31I	GGTCTC	1 cut(s)	1808
Eco47I	GGWCC	3 cut(s)	350, 1705, 2233
Eco53kI	GAGCTC	1 cut(s)	2146
Eco57I	CTGAAG	4 cut(s)	896, 983, 1553, 1575
Eco91I	GGTNACC	1 cut(s)	967
EcoICRI	GAGCTC	1 cut(s)	2146
EcoO65I	GGTNACC	1 cut(s)	967
EcoRII	CCWGG	3 cut(s)	1428, 1440, 1817
EcoT14I	CCWWGG	6 cut(s)	304, 729, 786, 1167, 1956, 2151
EcoT38I	GRGCYC	1 cut(s)	2148
ErhI	CCWWGG	6 cut(s)	304, 729, 786, 1167, 1956, 2151
FalI	AAGNNNNNCTT	6 cut(s)	81, 113, 2118, 2150, 2144, 2176
FaqI	GGGAC	5 cut(s)	344, 435, 922, 2194, 2408
FbaI	TGATCA	2 cut(s)	426, 1849
FblI	GTMKAC	2 cut(s)	711, 2388
Fnu4HI	GCNGC	7 cut(s)	258, 290, 1266, 1565, 1767, 2506, 2509
FokI	GGATG	7 cut(s)	264, 410, 596, 779, 1150, 1349, 2018
FriOI	GRGCYC	1 cut(s)	2148
Fsp4HI	GCNGC	7 cut(s)	258, 290, 1266, 1565, 1767, 2506, 2509
FspBI	CTAG	3 cut(s)	305, 896, 912
GlaI	GCGC	2 cut(s)	1765, 2256
GluI	GCNGC	7 cut(s)	258, 290, 1266, 1565, 1767, 2506, 2509
GsaI	CCCAGC	2 cut(s)	162, 365
GsuI	CTGGAG	1 cut(s)	1840
HaeIII	GGCC	9 cut(s)	5, 117, 138, 309, 503, 608, 857, 1440, 1955
HapII	CCGG	8 cut(s)	135, 294, 328, 554, 1081, 1122, 1133, 2436
HgaI	GACGC	3 cut(s)	722, 2026, 2405
HhaI	GCGC	2 cut(s)	1766, 2257
Hin1I	GRCGYC	1 cut(s)	2397
Hin6I	GCGC	2 cut(s)	1764, 2255
HinP1I	GCGC	2 cut(s)	1764, 2255
HincII	GTYRAC	2 cut(s)	46, 532
HindII	GTYRAC	2 cut(s)	46, 532
HinfI	GANTC	4 cut(s)	975, 1221, 1286, 2072
HpaII	CCGG	8 cut(s)	135, 294, 328, 554, 1081, 1122, 1133, 2436
HphI	GGTGA	8 cut(s)	824, 832, 979, 1111, 1281, 1420, 1654, 1711
Hpy188III	TCNNGA	8 cut(s)	89, 593, 705, 1081, 1133, 1202, 2105, 2320
Hpy99I	CGWCG	2 cut(s)	378, 650
HpyAV	CCTTC	6 cut(s)	17, 958, 1396, 1629, 2002, 2271
HpyCH4III	ACNGT	5 cut(s)	301, 341, 547, 1607, 2455
HpyCH4IV	ACGT	3 cut(s)	448, 648, 1140
HpyF10VI	GCNNNNNNNGC	9 cut(s)	11, 119, 266, 614, 797, 1265, 1961, 2011, 2252
HpySE526I	ACGT	3 cut(s)	448, 648, 1140
Hsp92I	GRCGYC	1 cut(s)	2397
HspAI	GCGC	2 cut(s)	1764, 2255
Kpn2I	TCCGGA	2 cut(s)	1080, 1132
KroI	GCCGGC	1 cut(s)	134
KroNI	GCCGGC	1 cut(s)	136
Ksp22I	TGATCA	2 cut(s)	426, 1849
LmnI	GCTCC	2 cut(s)	2011, 2290
Lsp1109I	GCAGC	6 cut(s)	269, 1252, 1551, 1778, 2492, 2495
LweI	GCATC	6 cut(s)	286, 800, 1109, 1287, 1831, 2448
MaeI	CTAG	3 cut(s)	305, 896, 912
MaeII	ACGT	3 cut(s)	448, 648, 1140
MaeIII	GTNAC	4 cut(s)	79, 967, 1141, 1287
MboII	GAAGA	7 cut(s)	1265, 1384, 1693, 1820, 1823, 1919, 2083
MfeI	CAATTG	2 cut(s)	1269, 1536
MflI	RGATCY	1 cut(s)	1920
MhlI	GDGCHC	7 cut(s)	220, 271, 631, 1728, 2148, 2248, 2295
MlsI	TGGCCA	1 cut(s)	857
MluCI	AATT	6 cut(s)	420, 672, 815, 1269, 1458, 1536
MluNI	TGGCCA	1 cut(s)	857
MlyI	GAGTC	3 cut(s)	1215, 1295, 2066
Mox20I	TGGCCA	1 cut(s)	857
MroI	TCCGGA	2 cut(s)	1080, 1132
MroNI	GCCGGC	1 cut(s)	134
MroXI	GAANNNNTTC	1 cut(s)	2315
MscI	TGGCCA	1 cut(s)	857
MseI	TTAA	6 cut(s)	410, 951, 1653, 1863, 1914, 2043
MslI	CAYNNNNRTG	3 cut(s)	1388, 1731, 2192
Msp20I	TGGCCA	1 cut(s)	857
MspA1I	CMGCKG	2 cut(s)	361, 2505
MspCI	CTTAAG	1 cut(s)	409
MspI	CCGG	8 cut(s)	135, 294, 328, 554, 1081, 1122, 1133, 2436
MspR9I	CCNGG	6 cut(s)	294, 329, 555, 1430, 1442, 1819
MunI	CAATTG	2 cut(s)	1269, 1536
Mva1269I	GAATGC	2 cut(s)	115, 1259
MvaI	CCWGG	3 cut(s)	1430, 1442, 1819
MvnI	CGCG	2 cut(s)	2037, 2255
MwoI	GCNNNNNNNGC	9 cut(s)	11, 119, 266, 614, 797, 1265, 1961, 2011, 2252
NaeI	GCCGGC	1 cut(s)	136
NciI	CCSGG	3 cut(s)	294, 329, 555
NcoI	CCATGG	1 cut(s)	729
NgoMIV	GCCGGC	1 cut(s)	134
NlaIV	GGNNCC	3 cut(s)	1533, 1707, 1922
NmeAIII	GCCGAG	1 cut(s)	335
NmuCI	GTSAC	3 cut(s)	79, 967, 1287
NspI	RCATGY	2 cut(s)	2116, 2299
PaqCI	CACCTGC	1 cut(s)	2368
PciI	ACATGT	1 cut(s)	2295
PcsI	WCGNNNNNNNCGW	1 cut(s)	642
PctI	GAATGC	2 cut(s)	115, 1259
PdiI	GCCGGC	1 cut(s)	136
PdmI	GAANNNNTTC	1 cut(s)	2315
PfeI	GAWTC	1 cut(s)	975
PflFI	GACNNNGTC	1 cut(s)	2455
PfoI	TCCNGGA	1 cut(s)	553
PkrI	GCNGC	7 cut(s)	259, 291, 1267, 1566, 1768, 2507, 2510
PleI	GAGTC	3 cut(s)	1215, 1294, 2066
PpsI	GAGTC	3 cut(s)	1215, 1294, 2066
Ppu21I	YACGTR	1 cut(s)	1141
PscI	ACATGT	1 cut(s)	2295
Psp124BI	GAGCTC	1 cut(s)	2148
Psp6I	CCWGG	3 cut(s)	1428, 1440, 1817
PspEI	GGTNACC	1 cut(s)	967
PspFI	CCCAGC	2 cut(s)	158, 361
PspGI	CCWGG	3 cut(s)	1428, 1440, 1817
PspN4I	GGNNCC	3 cut(s)	1533, 1707, 1922
PspPI	GGNCC	5 cut(s)	4, 350, 606, 1705, 2233
PsrI	GAACNNNNNNTAC	2 cut(s)	2483, 2515
PsuI	RGATCY	1 cut(s)	1920
PsyI	GACNNNGTC	1 cut(s)	2455
PvuII	CAGCTG	1 cut(s)	361
RseI	CAYNNNNRTG	3 cut(s)	1388, 1731, 2192
Rsr2I	CGGWCCG	1 cut(s)	2233
RsrII	CGGWCCG	1 cut(s)	2233
SacI	GAGCTC	1 cut(s)	2148
SaqAI	TTAA	6 cut(s)	410, 951, 1653, 1863, 1914, 2043
SatI	GCNGC	7 cut(s)	258, 290, 1266, 1565, 1767, 2506, 2509
Sau96I	GGNCC	5 cut(s)	4, 350, 606, 1705, 2233
SchI	GAGTC	3 cut(s)	1215, 1295, 2066
ScrFI	CCNGG	6 cut(s)	294, 329, 555, 1430, 1442, 1819
SduI	GDGCHC	7 cut(s)	220, 271, 631, 1728, 2148, 2248, 2295
SfaNI	GCATC	6 cut(s)	286, 800, 1109, 1287, 1831, 2448
SfcI	CTRYAG	1 cut(s)	546
SinI	GGWCC	3 cut(s)	350, 1705, 2233
SmiMI	CAYNNNNRTG	3 cut(s)	1388, 1731, 2192
SmlI	CTYRAG	5 cut(s)	171, 409, 1202, 1710, 2105
SmoI	CTYRAG	5 cut(s)	171, 409, 1202, 1710, 2105
SnaBI	TACGTA	1 cut(s)	1141
SpeI	ACTAGT	1 cut(s)	911
Sse9I	AATT	6 cut(s)	420, 672, 815, 1269, 1458, 1536
SsiI	CCGC	4 cut(s)	290, 1505, 2035, 2503
SspMI	CTAG	3 cut(s)	305, 896, 912
SstI	GAGCTC	1 cut(s)	2148
StyD4I	CCNGG	6 cut(s)	292, 327, 553, 1428, 1440, 1817
StyI	CCWWGG	6 cut(s)	304, 729, 786, 1167, 1956, 2151
TaaI	ACNGT	5 cut(s)	301, 341, 547, 1607, 2455
TaiI	ACGT	3 cut(s)	451, 651, 1143
TaqI	TCGA	5 cut(s)	498, 645, 1182, 2070, 2075
TaqII	GACCGA	1 cut(s)	2250
TasI	AATT	6 cut(s)	420, 672, 815, 1269, 1458, 1536
TatI	WGTACW	6 cut(s)	936, 1242, 1452, 1473, 1647, 1884
TauI	GCSGC	1 cut(s)	292
TfiI	GAWTC	1 cut(s)	975
Tru1I	TTAA	6 cut(s)	410, 951, 1653, 1863, 1914, 2043
Tru9I	TTAA	6 cut(s)	410, 951, 1653, 1863, 1914, 2043
TseFI	GTSAC	3 cut(s)	79, 967, 1287
TseI	GCWGC	6 cut(s)	257, 1265, 1564, 1766, 2505, 2508
Tsp45I	GTSAC	3 cut(s)	79, 967, 1287
TspDTI	ATGAA	7 cut(s)	459, 527, 684, 783, 1242, 1266, 2253
TspGWI	ACGGA	1 cut(s)	2246
Tth111I	GACNNNGTC	1 cut(s)	2455
Vha464I	CTTAAG	1 cut(s)	409
VneI	GTGCAC	2 cut(s)	216, 1724
VpaK11BI	GGWCC	3 cut(s)	350, 1705, 2233
XapI	RAATTY	3 cut(s)	420, 672, 815
XceI	RCATGY	2 cut(s)	2116, 2299
XcmI	CCANNNNNNNNNTGG	1 cut(s)	850
XmaJI	CCTAGG	1 cut(s)	304
XmiI	GTMKAC	2 cut(s)	711, 2388
XmnI	GAANNNNTTC	1 cut(s)	2315
XspI	CTAG	3 cut(s)	305, 896, 912

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.