Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0002043

Physical Location & Seq

Genomic Coordinates Reverse (-)

55078939 .. 55082363

Gene Structure 3425 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00373990.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2550 bp

ATGGCCCTTCAACCTCAAACCCCTAGGGTCAACTTTCAAAGCACCCTTGCAGCACCTCGTCAAGACACCCTTGCACCTCAGAGTGCCGGCCTTGTACCTCAAACTGCCGCCCTTGTGCCTCAACAAAATCCCAGCCTAGTGCCTCAACAAAATCCCAGCCTTGTGCCTCAACAAAATCCCAGCCTTATGCCTCAAAGTACTGCCCTTGTACCTCAAAGTGCCCCTGCACCTCAAAGTGCCCTTGTGCCTCAAGCTAGGGCGCCCGCTGCTGTCCTAGGCCGAGGAGGAGACAACTACCCGGGAAGAGTAGGAGACAACTACCCTGGAAGAGGAGGAGACAACTACCTAGGCAGAGGAGGAGACGGCTACCGTACTACTCCAGCTACTCAGCTTGGTAGAGATCGTCGTCAGTATTCAGCATCATCAGATGACAATACATTGACCAACCAAATTCTGATGACTGATAGATCGCATGAACGTCCCTACGATGTGCCTCTTCTATCTGTCAAGCAGGTTCTCCAAACTATCGAGGCCATTTTGGCTCGTGTCAACAAACCACTTGACATCCATGGCACTGTAGTTCCGGGAGGTCTCGTACCTGCAACTGCACATGTGGATACTTTGGAACATGAGAAGGCCATCCATGCTAGCCTCAGTAGCCTCCACGACAGCATCGATGTTCCAACCAGCATTCTTAATGAAATTTCCAGCGAGATACTTAGCAAGTCGCTAACAGGGGTAGATGCAAGCAAGACCACCATGGATATTTTAGAGAGGGTCCAGCACTACGATTGGGACGAAAAGGTGGTGCTTGCCTTGGCATCTTTTGCGATCAAAGATGGTGAATTTTGGCTTGTGGCTCAGCTTTTCACCACCAACGCACTGGCCAAAGCCATCGGACAACTGAAGCAAATGCAAGAGATACTAGAACGATCTGGGACTAGTTTAAAACCGAAGTTTGAAGCATACAACAATCTAGTTAGAGCCGTGCTCAAGGTGACCAAGATCGTTGTTCATTTACAGGACCTTCACCGCGACCCCCACTTAACTGCTGAAATCAAAGCTACATCTCCCACTAATCATATCCCCACTGCTGTTTACTGGACTGTCAGGAGTATAGTCATTCTTGCATCACAACTTTTAGGGATCACTGGCTCTGATCCGGAGTACGTAACAGAGGCATGGGAACTATCATCCTTGGCACACAAGCTCGAAAACATATACAACCATCTTGAGGAGAACCTAAACAGACTCTATCAACTCATTCAGAAGTTCAGAGATGAAGAAGCATTCAACGCAATTGCACGCATCTTAGAGTCACCACATATTGATAACTCCAAGCCTCTGAGGGTTTTGTTTTACAAGGATGACCAACCTGCACTCTATGATGGCTACAACAAGAAGAGGGTGGACATTGATGTGCTGAGGAGGAAGGTGGTGATACTGATGATTTCAGACCTGGACATCACTCAGGAAAATGAGTACCTGATTGCTCATCAAATGTACTCGGAGAAACGCCAGTTCCCACAAAGGCCAGAGAGCCAGTACGAGATTGTCTGGGTTCCAATTGTGGACAATTGGACTGAAGCCAAGCAGCTGCAGTTTGAGGAACTTAGAAATGGCATGGAATGGTACACTGTGTACCACCCTTCGGTTGTGTCTCCGATTGTGGTGAAGTACATCAGGCACCAGAAGAAATGGAACTTTGTGAAGAAGCCTCTGCTTGTGGTGATGGACCCTCAAGGCAAAATAGTGCACACCAATGCTGTTCACATGATGTGTGTTTTCGGAAGTGCAGCATACCCATTCACAAGCAACAGAGAGAGATTGCTTTGGGAAGAAGAGACCTGGAGGATTGAGCTTTTGGCAGATGCCATCGATCAAACTCTACTTAACTGGATTTCGGAAAGGAAGTACATTTGCTTGTATGGCGGGGAAGACATTAACTGGATCCGAAGCTTCACAAGGTCTGCAAAAGAAGTAGCCTTGGAAGCTGGTATCGAATTAGAGTTGCTCTATCTTGGCAAGAGCAAGCAGAAGGAGCGAGTGGCCAGGGACATCATCCGTGTTATTAAGGAGGAGAGGCTAAGCCACACCATCGACTGGAACCTCATTTGGTTCTTCTGGGTTCGTCTTGAGAGCATGTGGCAGTCCAAAGGACAGCTTATGAGTGAGCTGTCCAGGGCTAACTTCAGGGACGATAACATAAAAAACGACATCATAATGCAGGGGATTGTGTCACTGCTGAGCTTCGGTTCCAGCGATCGGGGGTGGGCTCTGATCGGGCTTCCTTCATCAGACATGTCTAAGGGTAATGGGGATCACATGTTGAAGAGCATGAACGACTTCAAGATTTGGAAGATTAGGCACAATGAGATGGGGTTCACACCTGCACTGAATGAATATCTTCTTGGGGTGTACAAGAGCGCCCCACATCACTGCACCAGTCTCATACTGCCTGCAACCGGCATCATGCCTGAGACTGTGGCCTGTGCCGAATGTGGTCGTCTCATGGAGAGGTACGCCATGTTCCGCTGCTGCACTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0008150 GO:0009888 GO:0010087 GO:0010088 GO:0032502 GO:0042802 GO:0042803 GO:0043621 GO:0046983 GO:0048856

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SEO_C SEO_N

Protein Analysis

849

Amino Acids

96.1

Weight (kDa)

6.34

Isoelectric Point (pI)

48.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SEO_N	PF14576	142 - 442	3.2e-70	Sieve element occlusion N-terminus
SEO_C	PF14577	607 - 848	1.3e-81	Sieve element occlusion C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002043)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g25290 FvH4_4g25290 FvH4_4g25290 FvH4_4g25310 FvH4_4g25320
malus_domestica	MD13G1105000.v1.1 MD13G1105100.v1.1 MD16G1104800.v1.1 MD16G1105000.v1.1
prunus_persica	Prupe.1G241400_v2.0.a1 Prupe.1G241500_v2.0.a1
pyrus_communis	pycom13g09110 pycom13g09120 pycom16g08950 pycom16g08960
rosa_chinensis	RchiOBHm_Chr4g0432561 RchiOBHm_Chr4g0432571 RchiOBHm_Chr4g0432611
rosa_laevigata	RLG00000006831 RLG00000006832 RLG00000006833
rosa_multiflora	Rmu_sc0003886.1_g000001 Rmu_sc0004612.1_g000014 Rmu_sc0027699.1_g000001
rosa_roxburghii	Rroxscaffold_5G00373990 Rroxscaffold_5G00374000 Rroxscaffold_5G00374030
rosa_rugosa	Rorug04G0260100 Rorug04G0260100 Rorug04G0260200
rosa_samantha	Rh4AG315100 Rh4AG315200 Rh4AG315300 Rh4BG322800 Rh4BG322900 Rh4BG323000 Rh4CG337900 Rh4CG338000 Rh4CG338100 Rh4DG318600 Rh4DG318700 Rh4DG318800
rosa_wichuraiana	Rw4G027380 Rw4G027390 Rw4G027420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2398
Acc36I	ACCTGC	4 cut(s)	502, 607, 1384, 2398
AccB1I	GGYRCC	2 cut(s)	259, 1686
AccB7I	CCANNNNNTGG	1 cut(s)	2103
AccII	CGCG	1 cut(s)	1035
AccIII	TCCGGA	1 cut(s)	1162
AciI	CCGC	5 cut(s)	108, 264, 1033, 1932, 2533
AclWI	GGATC	5 cut(s)	1154, 1154, 1945, 1958, 2328
AcoI	YGGCCR	2 cut(s)	885, 2049
AcsI	RAATTY	3 cut(s)	450, 702, 845
AcuI	CTGAAG	3 cut(s)	926, 1605, 2176
AcyI	GRCGYC	1 cut(s)	260
AfiI	CCNNNNNNNGG	6 cut(s)	329, 1234, 1651, 2103, 2265, 2465
AflIII	ACRYGT	3 cut(s)	610, 2301, 2325
AgsI	TTSAA	6 cut(s)	11, 38, 962, 1294, 2332, 2350
AhlI	ACTAGT	1 cut(s)	941
AjnI	CCWGG	5 cut(s)	322, 1458, 1847, 2051, 2180
AjuI	GAANNNNNNNTTGG	2 cut(s)	2394, 2426
Alw21I	GWGCWC	2 cut(s)	993, 1758
Alw44I	GTGCAC	1 cut(s)	1754
AlwI	GGATC	5 cut(s)	1154, 1154, 1945, 1958, 2328
Ama87I	CYCGRG	1 cut(s)	298
Aor13HI	TCCGGA	1 cut(s)	1162
AoxI	GGCC	9 cut(s)	3, 88, 277, 531, 636, 885, 1532, 2049, 2487
ApaLI	GTGCAC	1 cut(s)	1754
ApeKI	GCWGC	7 cut(s)	50, 266, 1594, 1597, 1796, 2535, 2538
ApoI	RAATTY	3 cut(s)	450, 702, 845
Asp700I	GAANNNNTTC	2 cut(s)	2345, 2406
AspA2I	CCTAGG	3 cut(s)	23, 274, 346
AspLEI	GCGC	2 cut(s)	262, 2429
AspS9I	GGNCC	4 cut(s)	4, 778, 1024, 1735
AsuC2I	CCSGG	3 cut(s)	299, 300, 585
AsuHPI	GGTGA	8 cut(s)	854, 862, 1009, 1022, 1311, 1450, 1684, 1741
AsuNHI	GCTAGC	1 cut(s)	647
AvaI	CYCGRG	1 cut(s)	298
AvaII	GGWCC	3 cut(s)	778, 1024, 1735
AvrII	CCTAGG	3 cut(s)	23, 274, 346
BaeGI	GKGCMC	3 cut(s)	223, 241, 1758
BalI	TGGCCA	2 cut(s)	887, 2051
BamHI	GGATCC	1 cut(s)	1950
BanI	GGYRCC	2 cut(s)	259, 1686
BanII	GRGCYC	1 cut(s)	2278
BauI	CACGAG	1 cut(s)	543
BbsI	GAAGAC	1 cut(s)	1944
Bbv12I	GWGCWC	2 cut(s)	993, 1758
BbvCI	CCTCAGC	1 cut(s)	1424
BbvI	GCAGC	7 cut(s)	62, 253, 1584, 1606, 1808, 2522, 2525
BccI	CCATC	9 cut(s)	647, 833, 902, 1236, 1382, 1726, 1883, 2105, 2371
BceAI	ACGGC	2 cut(s)	379, 971
BciT130I	CCWGG	5 cut(s)	324, 1460, 1849, 2053, 2182
BciVI	GTATCC	1 cut(s)	610
BcnI	CCSGG	3 cut(s)	299, 300, 585
BcuI	ACTAGT	1 cut(s)	941
BfaI	CTAG	9 cut(s)	24, 137, 255, 275, 347, 648, 926, 942, 977
BfmI	CTRYAG	2 cut(s)	576, 1598
BfoI	RGCGCY	2 cut(s)	263, 2430
BfuAI	ACCTGC	4 cut(s)	502, 607, 1384, 2398
BfuI	GTATCC	1 cut(s)	610
BglI	GCCNNNNNGGC	1 cut(s)	539
BisI	GCNGC	8 cut(s)	51, 108, 267, 1595, 1598, 1797, 2536, 2539
BlnI	CCTAGG	3 cut(s)	23, 274, 346
BlpI	GCTNAGC	3 cut(s)	861, 2087, 2246
BlsI	GCNGC	8 cut(s)	52, 109, 268, 1596, 1599, 1798, 2537, 2540
BmcAI	AGTACT	1 cut(s)	199
Bme1390I	CCNGG	8 cut(s)	299, 300, 324, 585, 1460, 1849, 2053, 2182
Bme18I	GGWCC	3 cut(s)	778, 1024, 1735
BmeT110I	CYCGRG	1 cut(s)	298
BmgT120I	GGNCC	4 cut(s)	4, 778, 1024, 1735
BmiI	GGNNCC	8 cut(s)	261, 779, 1563, 1688, 1737, 1952, 2108, 2257
BmrFI	CCNGG	8 cut(s)	299, 300, 324, 585, 1460, 1849, 2053, 2182
BmsI	GCATC	8 cut(s)	428, 681, 733, 830, 1139, 1317, 1861, 2478
BmtI	GCTAGC	1 cut(s)	651
BpiI	GAAGAC	1 cut(s)	1944
BplI	GAGNNNNNCTC	2 cut(s)	975, 1007
BpmI	CTGGAG	2 cut(s)	363, 1870
Bpu10I	CCTNAGC	1 cut(s)	1424
Bpu1102I	GCTNAGC	3 cut(s)	861, 2087, 2246
BpuEI	CTTGAG	5 cut(s)	234, 977, 1253, 1725, 2156
BpuMI	CCSGG	3 cut(s)	299, 300, 585
Bsa29I	ATCGAT	2 cut(s)	675, 1878
BsaAI	YACGTR	1 cut(s)	1171
BsaHI	GRCGYC	1 cut(s)	260
BsaI	GGTCTC	2 cut(s)	596, 1838
BsaWI	WCCGGW	1 cut(s)	1162
BsaXI	ACNNNNNCTCC	2 cut(s)	579, 609
Bsc4I	CCNNNNNNNGG	6 cut(s)	329, 1234, 1651, 2103, 2265, 2465
Bse118I	RCCGGY	2 cut(s)	86, 2465
Bse1I	ACTGG	9 cut(s)	888, 1106, 1156, 1519, 1543, 1901, 1952, 2108, 2445
BseAI	TCCGGA	1 cut(s)	1162
BseBI	CCWGG	5 cut(s)	324, 1460, 1849, 2053, 2182
BseCI	ATCGAT	2 cut(s)	675, 1878
BseGI	GGATG	5 cut(s)	564, 639, 1193, 1372, 2061
BseLI	CCNNNNNNNGG	6 cut(s)	329, 1234, 1651, 2103, 2265, 2465
BseMII	CTCAG	9 cut(s)	92, 401, 667, 875, 1337, 1415, 1484, 2237, 2469
BseNI	ACTGG	9 cut(s)	888, 1106, 1156, 1519, 1543, 1901, 1952, 2108, 2445
BseRI	GAGGAG	9 cut(s)	297, 300, 345, 348, 369, 372, 1250, 1441, 2093
BseSI	GKGCMC	3 cut(s)	223, 241, 1758
BseXI	GCAGC	7 cut(s)	62, 253, 1584, 1606, 1808, 2522, 2525
BseYI	CCCAGC	3 cut(s)	131, 155, 179
BsgI	GTGCAG	7 cut(s)	210, 591, 1362, 1815, 2376, 2425, 2524
Bsh1236I	CGCG	1 cut(s)	1035
Bsh1285I	CGRYCG	1 cut(s)	2266
BshFI	GGCC	9 cut(s)	5, 90, 279, 533, 638, 887, 1534, 2051, 2489
BshNI	GGYRCC	2 cut(s)	259, 1686
BshVI	ATCGAT	2 cut(s)	675, 1878
BsiEI	CGRYCG	1 cut(s)	2266
BsiHKAI	GWGCWC	2 cut(s)	993, 1758
BsiHKCI	CYCGRG	1 cut(s)	298
BsiSI	CCGG	5 cut(s)	87, 299, 584, 1163, 2466
BslFI	GGGAC	5 cut(s)	465, 809, 952, 2069, 2210
BslI	CCNNNNNNNGG	6 cut(s)	329, 1234, 1651, 2103, 2265, 2465
BsmBI	CGTCTC	2 cut(s)	354, 2513
BsmFI	GGGAC	5 cut(s)	465, 809, 952, 2069, 2210
BsmI	GAATGC	2 cut(s)	690, 1289
BsnI	GGCC	9 cut(s)	5, 90, 279, 533, 638, 887, 1534, 2051, 2489
Bso31I	GGTCTC	2 cut(s)	596, 1838
BsoBI	CYCGRG	1 cut(s)	298
Bsp1286I	GDGCHC	5 cut(s)	223, 241, 993, 1758, 2278
Bsp13I	TCCGGA	1 cut(s)	1162
Bsp1407I	TGTACA	1 cut(s)	2418
Bsp1720I	GCTNAGC	3 cut(s)	861, 2087, 2246
Bsp19I	CCATGG	2 cut(s)	568, 759
BspACI	CCGC	5 cut(s)	108, 264, 1033, 1932, 2533
BspANI	GGCC	9 cut(s)	5, 90, 279, 533, 638, 887, 1534, 2051, 2489
BspCNI	CTCAG	9 cut(s)	91, 400, 666, 874, 1338, 1416, 1483, 2238, 2470
BspDI	ATCGAT	2 cut(s)	675, 1878
BspEI	TCCGGA	1 cut(s)	1162
BspFNI	CGCG	1 cut(s)	1035
BspLI	GGNNCC	8 cut(s)	261, 779, 1563, 1688, 1737, 1952, 2108, 2257
BspMAI	CTGCAG	1 cut(s)	1602
BspMI	ACCTGC	4 cut(s)	502, 607, 1384, 2398
BspOI	GCTAGC	1 cut(s)	651
BspPI	GGATC	5 cut(s)	1154, 1154, 1945, 1958, 2328
BspQI	GCTCTTC	1 cut(s)	2327
BspT107I	GGYRCC	2 cut(s)	259, 1686
BspTNI	GGTCTC	2 cut(s)	596, 1838
BsrFI	RCCGGY	2 cut(s)	86, 2465
BsrGI	TGTACA	1 cut(s)	2418
BsrI	ACTGG	9 cut(s)	888, 1106, 1156, 1519, 1543, 1901, 1952, 2108, 2445
BssAI	RCCGGY	2 cut(s)	86, 2465
BssNI	GRCGYC	1 cut(s)	260
BssSI	CACGAG	1 cut(s)	543
BssT1I	CCWWGG	8 cut(s)	23, 274, 346, 568, 759, 816, 1197, 1986
Bst2BI	CACGAG	1 cut(s)	543
Bst2UI	CCWGG	5 cut(s)	324, 1460, 1849, 2053, 2182
Bst4CI	ACNGT	5 cut(s)	371, 577, 1108, 1639, 2485
Bst6I	CTCTTC	6 cut(s)	298, 322, 501, 1397, 1836, 2327
BstACI	GRCGYC	1 cut(s)	260
BstAPI	GCANNNNNTGC	1 cut(s)	827
BstAUI	TGTACA	1 cut(s)	2418
BstBAI	YACGTR	1 cut(s)	1171
BstC8I	GCNNGC	8 cut(s)	88, 264, 649, 748, 813, 1306, 2033, 2460
BstDSI	CCRYGG	2 cut(s)	568, 759
BstEII	GGTNACC	1 cut(s)	997
BstENI	CCTNNNNNAGG	1 cut(s)	327
BstF5I	GGATG	5 cut(s)	564, 639, 1193, 1372, 2061
BstFNI	CGCG	1 cut(s)	1035
BstH2I	RGCGCY	2 cut(s)	263, 2430
BstHHI	GCGC	2 cut(s)	262, 2429
BstMCI	CGRYCG	1 cut(s)	2266
BstMWI	GCNNNNNNNGC	9 cut(s)	266, 539, 644, 657, 827, 1295, 1929, 1991, 2041
BstNI	CCWGG	5 cut(s)	324, 1460, 1849, 2053, 2182
BstNSI	RCATGY	4 cut(s)	614, 2146, 2305, 2329
BstPI	GGTNACC	1 cut(s)	997
BstSCI	CCNGG	8 cut(s)	297, 298, 322, 583, 1458, 1847, 2051, 2180
BstSFI	CTRYAG	2 cut(s)	576, 1598
BstSLI	GKGCMC	3 cut(s)	223, 241, 1758
BstSNI	TACGTA	1 cut(s)	1171
BstUI	CGCG	1 cut(s)	1035
BstV1I	GCAGC	7 cut(s)	62, 253, 1584, 1606, 1808, 2522, 2525
BstV2I	GAAGAC	1 cut(s)	1944
BstX2I	RGATCY	1 cut(s)	1950
BstXI	CCANNNNNNTGG	1 cut(s)	883
BstYI	RGATCY	1 cut(s)	1950
Bsu15I	ATCGAT	2 cut(s)	675, 1878
BsuI	GTATCC	1 cut(s)	610
BsuRI	GGCC	9 cut(s)	5, 90, 279, 533, 638, 887, 1534, 2051, 2489
BsuTUI	ATCGAT	2 cut(s)	675, 1878
BtgI	CCRYGG	2 cut(s)	568, 759
BtsCI	GGATG	5 cut(s)	564, 639, 1193, 1372, 2061
BtsI	GCAGTG	3 cut(s)	1089, 2240, 2437
BtsIMutI	CAGTG	9 cut(s)	573, 881, 1089, 1149, 1635, 2240, 2393, 2437, 2541
BveI	ACCTGC	4 cut(s)	502, 607, 1384, 2398
Cac8I	GCNNGC	8 cut(s)	88, 264, 649, 748, 813, 1306, 2033, 2460
CfoI	GCGC	2 cut(s)	262, 2429
Cfr10I	RCCGGY	2 cut(s)	86, 2465
Cfr13I	GGNCC	4 cut(s)	4, 778, 1024, 1735
Cfr9I	CCCGGG	1 cut(s)	298
ClaI	ATCGAT	2 cut(s)	675, 1878
DinI	GGCGCC	1 cut(s)	261
DraI	TTTAAA	1 cut(s)	948
EaeI	YGGCCR	2 cut(s)	885, 2049
Eam1104I	CTCTTC	6 cut(s)	298, 322, 501, 1397, 1836, 2327
EarI	CTCTTC	6 cut(s)	298, 322, 501, 1397, 1836, 2327
Eco105I	TACGTA	1 cut(s)	1171
Eco130I	CCWWGG	8 cut(s)	23, 274, 346, 568, 759, 816, 1197, 1986
Eco24I	GRGCYC	1 cut(s)	2278
Eco31I	GGTCTC	2 cut(s)	596, 1838
Eco47I	GGWCC	3 cut(s)	778, 1024, 1735
Eco57I	CTGAAG	3 cut(s)	926, 1605, 2176
Eco88I	CYCGRG	1 cut(s)	298
Eco91I	GGTNACC	1 cut(s)	997
EcoNI	CCTNNNNNAGG	1 cut(s)	327
EcoO109I	RGGNCCY	1 cut(s)	1024
EcoO65I	GGTNACC	1 cut(s)	997
EcoRII	CCWGG	5 cut(s)	322, 1458, 1847, 2051, 2180
EcoT14I	CCWWGG	8 cut(s)	23, 274, 346, 568, 759, 816, 1197, 1986
EcoT38I	GRGCYC	1 cut(s)	2278
EgeI	GGCGCC	1 cut(s)	261
EheI	GGCGCC	1 cut(s)	261
ErhI	CCWWGG	8 cut(s)	23, 274, 346, 568, 759, 816, 1197, 1986
Esp3I	CGTCTC	2 cut(s)	354, 2513
FalI	AAGNNNNNCTT	4 cut(s)	54, 86, 2148, 2180
FaqI	GGGAC	5 cut(s)	465, 809, 952, 2069, 2210
FauI	CCCGC	2 cut(s)	271, 1925
Fnu4HI	GCNGC	8 cut(s)	51, 108, 267, 1595, 1598, 1797, 2536, 2539
FokI	GGATG	5 cut(s)	551, 626, 1180, 1379, 2048
FriOI	GRGCYC	1 cut(s)	2278
Fsp4HI	GCNGC	8 cut(s)	51, 108, 267, 1595, 1598, 1797, 2536, 2539
FspBI	CTAG	9 cut(s)	24, 137, 255, 275, 347, 648, 926, 942, 977
GlaI	GCGC	2 cut(s)	261, 2428
GluI	GCNGC	8 cut(s)	51, 108, 267, 1595, 1598, 1797, 2536, 2539
GsaI	CCCAGC	3 cut(s)	135, 159, 183
GsuI	CTGGAG	2 cut(s)	363, 1870
HaeII	RGCGCY	2 cut(s)	263, 2430
HaeIII	GGCC	9 cut(s)	5, 90, 279, 533, 638, 887, 1534, 2051, 2489
HapII	CCGG	5 cut(s)	87, 299, 584, 1163, 2466
HhaI	GCGC	2 cut(s)	262, 2429
Hin1I	GRCGYC	1 cut(s)	260
Hin6I	GCGC	2 cut(s)	260, 2427
HinP1I	GCGC	2 cut(s)	260, 2427
HincII	GTYRAC	2 cut(s)	31, 550
HindII	GTYRAC	2 cut(s)	31, 550
HindIII	AAGCTT	1 cut(s)	1957
HinfI	GANTC	2 cut(s)	1251, 1316
HpaII	CCGG	5 cut(s)	87, 299, 584, 1163, 2466
HphI	GGTGA	8 cut(s)	854, 862, 1009, 1022, 1311, 1450, 1684, 1741
Hpy188III	TCNNGA	7 cut(s)	62, 1111, 1163, 1232, 1472, 2135, 2350
Hpy99I	CGWCG	1 cut(s)	408
HpyAV	CCTTC	7 cut(s)	17, 628, 1037, 1426, 1659, 2032, 2301
HpyCH4III	ACNGT	5 cut(s)	371, 577, 1108, 1639, 2485
HpyCH4IV	ACGT	2 cut(s)	478, 1170
HpyF10VI	GCNNNNNNNGC	9 cut(s)	266, 539, 644, 657, 827, 1295, 1929, 1991, 2041
HpySE526I	ACGT	2 cut(s)	478, 1170
Hsp92I	GRCGYC	1 cut(s)	260
HspAI	GCGC	2 cut(s)	260, 2427
KasI	GGCGCC	1 cut(s)	259
Kpn2I	TCCGGA	1 cut(s)	1162
KroI	GCCGGC	1 cut(s)	86
KroNI	GCCGGC	1 cut(s)	88
LguI	GCTCTTC	1 cut(s)	2327
LmnI	GCTCC	1 cut(s)	2041
Lsp1109I	GCAGC	7 cut(s)	62, 253, 1584, 1606, 1808, 2522, 2525
LweI	GCATC	8 cut(s)	428, 681, 733, 830, 1139, 1317, 1861, 2478
MaeI	CTAG	9 cut(s)	24, 137, 255, 275, 347, 648, 926, 942, 977
MaeII	ACGT	2 cut(s)	478, 1170
MaeIII	GTNAC	4 cut(s)	997, 1171, 1317, 2238
MfeI	CAATTG	3 cut(s)	1299, 1566, 1576
MflI	RGATCY	1 cut(s)	1950
MhlI	GDGCHC	5 cut(s)	223, 241, 993, 1758, 2278
MlsI	TGGCCA	2 cut(s)	887, 2051
MluCI	AATT	7 cut(s)	450, 702, 845, 1299, 1566, 1576, 2003
MluNI	TGGCCA	2 cut(s)	887, 2051
Mly113I	GGCGCC	1 cut(s)	260
MlyI	GAGTC	2 cut(s)	1245, 1325
MmeI	TCCRAC	1 cut(s)	707
Mox20I	TGGCCA	2 cut(s)	887, 2051
MroI	TCCGGA	1 cut(s)	1162
MroNI	GCCGGC	1 cut(s)	86
MroXI	GAANNNNTTC	2 cut(s)	2345, 2406
MscI	TGGCCA	2 cut(s)	887, 2051
MseI	TTAA	6 cut(s)	696, 947, 1046, 1893, 1944, 2073
MslI	CAYNNNNRTG	3 cut(s)	1418, 1761, 2222
Msp20I	TGGCCA	2 cut(s)	887, 2051
MspA1I	CMGCKG	3 cut(s)	266, 1597, 2535
MspI	CCGG	5 cut(s)	87, 299, 584, 1163, 2466
MspR9I	CCNGG	8 cut(s)	299, 300, 324, 585, 1460, 1849, 2053, 2182
MunI	CAATTG	3 cut(s)	1299, 1566, 1576
Mva1269I	GAATGC	2 cut(s)	690, 1289
MvaI	CCWGG	5 cut(s)	324, 1460, 1849, 2053, 2182
MvnI	CGCG	1 cut(s)	1035
MwoI	GCNNNNNNNGC	9 cut(s)	266, 539, 644, 657, 827, 1295, 1929, 1991, 2041
NaeI	GCCGGC	1 cut(s)	88
NarI	GGCGCC	1 cut(s)	260
NciI	CCSGG	3 cut(s)	299, 300, 585
NcoI	CCATGG	2 cut(s)	568, 759
NgoMIV	GCCGGC	1 cut(s)	86
NheI	GCTAGC	1 cut(s)	647
NlaIV	GGNNCC	8 cut(s)	261, 779, 1563, 1688, 1737, 1952, 2108, 2257
NmeAIII	GCCGAG	1 cut(s)	305
NmuCI	GTSAC	3 cut(s)	997, 1317, 2238
NspI	RCATGY	4 cut(s)	614, 2146, 2305, 2329
PaqCI	CACCTGC	1 cut(s)	2398
PciI	ACATGT	3 cut(s)	610, 2301, 2325
PciSI	GCTCTTC	1 cut(s)	2327
PcsI	WCGNNNNNNNCGW	3 cut(s)	672, 795, 2259
PctI	GAATGC	2 cut(s)	690, 1289
PdiI	GCCGGC	1 cut(s)	88
PdmI	GAANNNNTTC	2 cut(s)	2345, 2406
PflMI	CCANNNNNTGG	1 cut(s)	2103
PfoI	TCCNGGA	1 cut(s)	583
PkrI	GCNGC	8 cut(s)	52, 109, 268, 1596, 1599, 1798, 2537, 2540
Ple19I	CGATCG	1 cut(s)	2266
PleI	GAGTC	2 cut(s)	1245, 1324
PluTI	GGCGCC	1 cut(s)	263
PpsI	GAGTC	2 cut(s)	1245, 1324
Ppu21I	YACGTR	1 cut(s)	1171
PpuMI	RGGWCCY	1 cut(s)	1024
PscI	ACATGT	3 cut(s)	610, 2301, 2325
Psp5II	RGGWCCY	1 cut(s)	1024
Psp6I	CCWGG	5 cut(s)	322, 1458, 1847, 2051, 2180
PspEI	GGTNACC	1 cut(s)	997
PspFI	CCCAGC	3 cut(s)	131, 155, 179
PspGI	CCWGG	5 cut(s)	322, 1458, 1847, 2051, 2180
PspN4I	GGNNCC	8 cut(s)	261, 779, 1563, 1688, 1737, 1952, 2108, 2257
PspPI	GGNCC	4 cut(s)	4, 778, 1024, 1735
PspPPI	RGGWCCY	1 cut(s)	1024
PsrI	GAACNNNNNNTAC	2 cut(s)	2513, 2545
PstI	CTGCAG	1 cut(s)	1602
PsuI	RGATCY	1 cut(s)	1950
PvuI	CGATCG	1 cut(s)	2266
PvuII	CAGCTG	1 cut(s)	1597
RseI	CAYNNNNRTG	3 cut(s)	1418, 1761, 2222
SapI	GCTCTTC	1 cut(s)	2327
SaqAI	TTAA	6 cut(s)	696, 947, 1046, 1893, 1944, 2073
SatI	GCNGC	8 cut(s)	51, 108, 267, 1595, 1598, 1797, 2536, 2539
Sau96I	GGNCC	4 cut(s)	4, 778, 1024, 1735
ScaI	AGTACT	1 cut(s)	199
SchI	GAGTC	2 cut(s)	1245, 1325
ScrFI	CCNGG	8 cut(s)	299, 300, 324, 585, 1460, 1849, 2053, 2182
SduI	GDGCHC	5 cut(s)	223, 241, 993, 1758, 2278
SfaNI	GCATC	8 cut(s)	428, 681, 733, 830, 1139, 1317, 1861, 2478
SfcI	CTRYAG	2 cut(s)	576, 1598
SfoI	GGCGCC	1 cut(s)	261
SinI	GGWCC	3 cut(s)	778, 1024, 1735
SmaI	CCCGGG	1 cut(s)	300
SmiMI	CAYNNNNRTG	3 cut(s)	1418, 1761, 2222
SmlI	CTYRAG	5 cut(s)	249, 992, 1232, 1740, 2135
SmoI	CTYRAG	5 cut(s)	249, 992, 1232, 1740, 2135
SnaBI	TACGTA	1 cut(s)	1171
SpeI	ACTAGT	1 cut(s)	941
Sse9I	AATT	7 cut(s)	450, 702, 845, 1299, 1566, 1576, 2003
SsiI	CCGC	5 cut(s)	108, 264, 1033, 1932, 2533
SspDI	GGCGCC	1 cut(s)	259
SspMI	CTAG	9 cut(s)	24, 137, 255, 275, 347, 648, 926, 942, 977
StyD4I	CCNGG	8 cut(s)	297, 298, 322, 583, 1458, 1847, 2051, 2180
StyI	CCWWGG	8 cut(s)	23, 274, 346, 568, 759, 816, 1197, 1986
TaaI	ACNGT	5 cut(s)	371, 577, 1108, 1639, 2485
TaiI	ACGT	2 cut(s)	481, 1173
TaqI	TCGA	6 cut(s)	528, 675, 1212, 1878, 2001, 2100
TasI	AATT	7 cut(s)	450, 702, 845, 1299, 1566, 1576, 2003
TatI	WGTACW	5 cut(s)	197, 1503, 1677, 1914, 2418
TauI	GCSGC	1 cut(s)	110
Tru1I	TTAA	6 cut(s)	696, 947, 1046, 1893, 1944, 2073
Tru9I	TTAA	6 cut(s)	696, 947, 1046, 1893, 1944, 2073
TscAI	CASTG	9 cut(s)	580, 888, 1096, 1156, 1642, 2247, 2400, 2444, 2548
TseFI	GTSAC	3 cut(s)	997, 1317, 2238
TseI	GCWGC	7 cut(s)	50, 266, 1594, 1597, 1796, 2535, 2538
Tsp45I	GTSAC	3 cut(s)	997, 1317, 2238
TspDTI	ATGAA	7 cut(s)	489, 714, 1004, 1296, 2283, 2354, 2415
TspGWI	ACGGA	1 cut(s)	2054
TspMI	CCCGGG	1 cut(s)	298
TspRI	CASTG	9 cut(s)	580, 888, 1096, 1156, 1642, 2247, 2400, 2444, 2548
Van91I	CCANNNNNTGG	1 cut(s)	2103
VneI	GTGCAC	1 cut(s)	1754
VpaK11BI	GGWCC	3 cut(s)	778, 1024, 1735
XagI	CCTNNNNNAGG	1 cut(s)	327
XapI	RAATTY	3 cut(s)	450, 702, 845
XceI	RCATGY	4 cut(s)	614, 2146, 2305, 2329
XcmI	CCANNNNNNNNNTGG	1 cut(s)	880
XmaI	CCCGGG	1 cut(s)	298
XmaJI	CCTAGG	3 cut(s)	23, 274, 346
XmnI	GAANNNNTTC	2 cut(s)	2345, 2406
XspI	CTAG	9 cut(s)	24, 137, 255, 275, 347, 648, 926, 942, 977
ZrmI	AGTACT	1 cut(s)	199

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.