Rorug04G0260200

Sieve element occlusion C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002043
Physical Location & Seq
Forward (+)
43255246 .. 43256528
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0260200.1

Sequence Viewer

Length: 489 bp
ATGGCAGAAAGCGCTAAGCCACTGATGCTAATTGCTATCGACGATAGTGAACACAGCTTTTATGCATTGGAGTGGACTCTGGACCATTTGTTTGCTCCCGGGTCCACCCATCCTTTCAAGCTCGGCATCATCCATGCTAGGGCATCTCCCGCTTCTGCTCTCGGAGTCGCCGGAGTTGGCTCTGGGGACTTCTTGAATTCGTTGATTTCTGATATGAAGAAATCGGCTACTAAGACGGTCGAAAAGGCCAAGGAAGTGTGCGCCACGAAAAAGGTGGAAATTGTGCTAGTGGAAGTTATGGAAGGAGATCCGAGAAATGTTATGCCCGAAGCTGTGGATAAACACAAAGCAGCCCTCTTGGTTCTCGGCAGTCATGGTTATGGAGCGGTCAAGAGGGCAGTTCTAGGGAGTGTAAGCGACTTCTGTGCTCATCATGCTAACTGTTCAGTGATGATTGTGAAGAGGCCAACCAAGCCTGCTGCCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008150 GO:0009888 GO:0010087 GO:0010088 GO:0032502 GO:0042802 GO:0042803 GO:0043621 GO:0046983 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

162

Amino Acids

17.19

Weight (kDa)

8.42

Isoelectric Point (pI)

24.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 9 - 154 1.7e-21 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 386
AciI CCGC 2 cut(s) 150, 386
AclWI GGATC 1 cut(s) 302
AcsI RAATTY 1 cut(s) 196
AfeI AGCGCT 1 cut(s) 13
AfiI CCNNNNNNNGG 1 cut(s) 139
AgsI TTSAA 2 cut(s) 118, 196
AluBI AGCT 3 cut(s) 57, 121, 332
AluI AGCT 3 cut(s) 57, 121, 332
Alw21I GWGCWC 1 cut(s) 430
AlwI GGATC 1 cut(s) 302
Ama87I CYCGRG 1 cut(s) 98
Aor51HI AGCGCT 1 cut(s) 13
AoxI GGCC 2 cut(s) 246, 464
ApeKI GCWGC 2 cut(s) 350, 479
ApoI RAATTY 1 cut(s) 196
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
AspLEI GCGC 2 cut(s) 14, 263
AspS9I GGNCC 2 cut(s) 82, 102
AsuC2I CCSGG 2 cut(s) 99, 100
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 2 cut(s) 82, 102
Bbv12I GWGCWC 1 cut(s) 430
BbvI GCAGC 2 cut(s) 362, 466
BccI CCATC 1 cut(s) 117
BcgI CGANNNNNNTGC 2 cut(s) 407, 441
BcnI CCSGG 2 cut(s) 99, 100
BfaI CTAG 3 cut(s) 138, 287, 404
BfoI RGCGCY 1 cut(s) 15
BisI GCNGC 2 cut(s) 351, 480
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 2 cut(s) 352, 481
Bme1390I CCNGG 2 cut(s) 99, 100
Bme18I GGWCC 2 cut(s) 82, 102
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 2 cut(s) 82, 102
BmiI GGNNCC 1 cut(s) 103
BmrFI CCNGG 2 cut(s) 99, 100
BmsI GCATC 3 cut(s) 15, 135, 152
Bpu1102I GCTNAGC 1 cut(s) 15
BpuMI CCSGG 2 cut(s) 99, 100
BsaJI CCNNGG 2 cut(s) 98, 249
Bsc4I CCNNNNNNNGG 1 cut(s) 139
BseDI CCNNGG 2 cut(s) 98, 249
BseGI GGATG 2 cut(s) 109, 129
BseLI CCNNNNNNNGG 1 cut(s) 139
BseXI GCAGC 2 cut(s) 362, 466
Bsh1285I CGRYCG 1 cut(s) 240
BshFI GGCC 2 cut(s) 248, 466
BsiEI CGRYCG 1 cut(s) 240
BsiHKAI GWGCWC 1 cut(s) 430
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 2 cut(s) 99, 171
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 1 cut(s) 139
BsmFI GGGAC 1 cut(s) 200
BsnI GGCC 2 cut(s) 248, 466
BsoBI CYCGRG 1 cut(s) 98
Bsp1286I GDGCHC 1 cut(s) 430
Bsp143I GATC 1 cut(s) 307
Bsp1720I GCTNAGC 1 cut(s) 15
BspACI CCGC 2 cut(s) 150, 386
BspANI GGCC 2 cut(s) 248, 466
BspLI GGNNCC 1 cut(s) 103
BspPI GGATC 1 cut(s) 302
BsrBI CCGCTC 1 cut(s) 386
BssECI CCNNGG 2 cut(s) 98, 249
BssMI GATC 1 cut(s) 307
BssT1I CCWWGG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 238, 443
Bst6I CTCTTC 1 cut(s) 455
BstC8I GCNNGC 1 cut(s) 477
BstDEI CTNAG 2 cut(s) 15, 231
BstF5I GGATG 2 cut(s) 109, 129
BstH2I RGCGCY 1 cut(s) 15
BstHHI GCGC 2 cut(s) 14, 263
BstKTI GATC 1 cut(s) 310
BstMBI GATC 1 cut(s) 307
BstMCI CGRYCG 1 cut(s) 240
BstMWI GCNNNNNNNGC 5 cut(s) 11, 25, 149, 434, 472
BstSCI CCNGG 2 cut(s) 97, 98
BstV1I GCAGC 2 cut(s) 362, 466
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BsuRI GGCC 2 cut(s) 248, 466
BtsCI GGATG 2 cut(s) 109, 129
BtsIMutI CAGTG 2 cut(s) 20, 453
Cac8I GCNNGC 1 cut(s) 477
CfoI GCGC 2 cut(s) 14, 263
Cfr13I GGNCC 2 cut(s) 82, 102
Cfr9I CCCGGG 1 cut(s) 98
CviAII CATG 3 cut(s) 134, 374, 434
DdeI CTNAG 2 cut(s) 15, 231
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
Eam1104I CTCTTC 1 cut(s) 455
EarI CTCTTC 1 cut(s) 455
Eco130I CCWWGG 1 cut(s) 249
Eco47I GGWCC 2 cut(s) 82, 102
Eco47III AGCGCT 1 cut(s) 13
Eco88I CYCGRG 1 cut(s) 98
EcoRI GAATTC 1 cut(s) 196
EcoT14I CCWWGG 1 cut(s) 249
EcoT22I ATGCAT 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 3 cut(s) 137, 377, 437
FaiI YATR 8 cut(s) 63, 135, 215, 299, 323, 375, 381, 435
FaqI GGGAC 1 cut(s) 200
FatI CATG 3 cut(s) 133, 373, 433
FauI CCCGC 1 cut(s) 157
Fnu4HI GCNGC 2 cut(s) 351, 480
FokI GGATG 2 cut(s) 96, 116
Fsp4HI GCNGC 2 cut(s) 351, 480
FspBI CTAG 3 cut(s) 138, 287, 404
GlaI GCGC 2 cut(s) 13, 262
GluI GCNGC 2 cut(s) 351, 480
HaeII RGCGCY 1 cut(s) 15
HaeIII GGCC 2 cut(s) 248, 466
HapII CCGG 2 cut(s) 99, 171
HhaI GCGC 2 cut(s) 14, 263
Hin1II CATG 3 cut(s) 137, 377, 437
Hin6I GCGC 2 cut(s) 12, 261
HinP1I GCGC 2 cut(s) 12, 261
HinfI GANTC 2 cut(s) 76, 165
HpaII CCGG 2 cut(s) 99, 171
Hpy166II GTNNAC 3 cut(s) 50, 75, 105
Hpy188I TCNGA 3 cut(s) 164, 211, 312
Hpy188III TCNNGA 3 cut(s) 80, 193, 391
Hpy8I GTNNAC 3 cut(s) 50, 75, 105
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 1 cut(s) 296
HpyCH4III ACNGT 2 cut(s) 238, 443
HpyCH4V TGCA 1 cut(s) 65
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 25, 149, 434, 472
HpyF3I CTNAG 2 cut(s) 15, 231
Hsp92II CATG 3 cut(s) 137, 377, 437
HspAI GCGC 2 cut(s) 12, 261
Kzo9I GATC 1 cut(s) 307
LmnI GCTCC 2 cut(s) 100, 383
LpnPI CCDG 4 cut(s) 65, 112, 168, 184
Lsp1109I GCAGC 2 cut(s) 362, 466
LweI GCATC 3 cut(s) 15, 135, 152
MaeI CTAG 3 cut(s) 138, 287, 404
MalI GATC 1 cut(s) 309
MbiI CCGCTC 1 cut(s) 386
MboI GATC 1 cut(s) 307
MboII GAAGA 2 cut(s) 229, 472
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 1 cut(s) 430
MluCI AATT 3 cut(s) 30, 196, 279
MlyI GAGTC 2 cut(s) 70, 174
MnlI CCTC 3 cut(s) 365, 387, 456
Mph1103I ATGCAT 1 cut(s) 67
MslI CAYNNNNRTG 2 cut(s) 70, 378
MspI CCGG 2 cut(s) 99, 171
MspR9I CCNGG 2 cut(s) 99, 100
MwoI GCNNNNNNNGC 5 cut(s) 11, 25, 149, 434, 472
NciI CCSGG 2 cut(s) 99, 100
NdeII GATC 1 cut(s) 307
NlaIII CATG 3 cut(s) 137, 377, 437
NlaIV GGNNCC 1 cut(s) 103
NmeAIII GCCGAG 2 cut(s) 102, 345
NsiI ATGCAT 1 cut(s) 67
PkrI GCNGC 2 cut(s) 352, 481
PleI GAGTC 2 cut(s) 70, 173
PpsI GAGTC 2 cut(s) 70, 173
PspN4I GGNNCC 1 cut(s) 103
PspPI GGNCC 2 cut(s) 82, 102
PsuI RGATCY 1 cut(s) 307
RseI CAYNNNNRTG 2 cut(s) 70, 378
SatI GCNGC 2 cut(s) 351, 480
Sau3AI GATC 1 cut(s) 307
Sau96I GGNCC 2 cut(s) 82, 102
SchI GAGTC 2 cut(s) 70, 174
ScrFI CCNGG 2 cut(s) 99, 100
SduI GDGCHC 1 cut(s) 430
SetI ASST 4 cut(s) 59, 123, 276, 334
SfaNI GCATC 3 cut(s) 15, 135, 152
SinI GGWCC 2 cut(s) 82, 102
SmaI CCCGGG 1 cut(s) 100
SmiMI CAYNNNNRTG 2 cut(s) 70, 378
Sse9I AATT 3 cut(s) 30, 196, 279
SsiI CCGC 2 cut(s) 150, 386
SspMI CTAG 3 cut(s) 138, 287, 404
StyD4I CCNGG 2 cut(s) 97, 98
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 2 cut(s) 238, 443
TaqI TCGA 2 cut(s) 39, 240
TasI AATT 3 cut(s) 30, 196, 279
TscAI CASTG 2 cut(s) 27, 453
TseI GCWGC 2 cut(s) 350, 479
TspDTI ATGAA 1 cut(s) 230
TspMI CCCGGG 1 cut(s) 98
TspRI CASTG 2 cut(s) 27, 453
VpaK11BI GGWCC 2 cut(s) 82, 102
XapI RAATTY 1 cut(s) 196
XcmI CCANNNNNNNNNTGG 1 cut(s) 271
XmaI CCCGGG 1 cut(s) 98
XspI CTAG 3 cut(s) 138, 287, 404
Zsp2I ATGCAT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.