Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002043

Physical Location & Seq

Genomic Coordinates Forward (+)

7535690 .. 7538880

Gene Structure 3191 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1105100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2235 bp

ATGGCACTGATACCTCTCAATAAGGGTGTCAACCCAACAAGCAGAGACGGCGGCCGTAATCGCGCTCCTCTGCTTCAAGATGGGCGCGACGGACATCGTCAGTATTCGACAACAGCTGATGATAATGCATTGATCAACCAAATTATGCTGACTGATAAATCTCATGACCATCCCTACGAAGTGCCTTCTCTTGCAGTCACACAAATTCTTCAAACCATTGAGGCCATTCTTTCACGCTTCACGAAACCTGACATCCATGATGGCACTCTAGTCCCGGGAGCATTAGTACCAGCAGCTATACATGCGGATTCTTTGGAGCATGAGAAGGCCCTCCATGCTAACCTGAGTACCCTCCACGACAGCTTCGATGTTCCAAACACCGTGTTCAATGCAATTCAATGCGAGTTAATGTCCAAATCGCTGCAAAGGGAAGATGCAAACAAGACCGCAATGGACATACTAGACATTGTGCAACACTACGATTGGGATGAAAAGGTGGTGCTTGCCTTGGGAGCATTTGCGATCAAAGATGGCGAGTTTTGGCTCCTGGCTCAGCAATACACCACCAACCCACTCGCCAAAGCAGTTGGACAGCTTAAGCAACTGCACGAGATCTTGGAACGTGCGGGGACTAGTTTGAAACCGAAGTTTGATGCATACAACAATCTGGTTAGGGTCATATTCAAAGTGACCAGGACCATTGTTCACTTACAGGAGATTCACAACGACCCCCATTTGAGCCCCGAAGTCAAAGCTACAGCTGCCACTGCTCATATCCCAACAGCTGTTTACTGGACAATTCGGGCTATTATAGTTGCTGCATCACAACTTCTGGGCATCACAGGCTCCGAGCCAGAGTACGTGACAGAGGCGTGGGAACTATCATCGCTGGCCCATAAGCTCGAAAACATATTCAATCACCTTGAAGAGATCCTCGACAGATTACATCAACTTATCCAGAAGAACAAAGACGAGGATGCTTTCAATGCAATTGCACGCATCTTGGAAACACCACACATTGATAACTCGAAGCCTCTGAAGGTTTTGTTTTACAAGGATGATCAGCCTGCACTCTATGATTGCTACAACAAGAAGAGGGTTGACATTGATGTGCTAAAAAGGAAAGTAGTGATTTTGTTCATCACAGACCTAGACGTTATCCACGAAAACGAGTACATGATTGTCCAACAAATGTACTTGGAAAAACAACACAACCCTACAAGGCCGGAGAGCCAGTACGAGGTTGTGTGGCTTCCAATTACAGATGTGTGGACTGACGCCAAGTACCAGCAATTCGAGATGCTAAGAAATAACATAGAATGGTACACCGTGTTCCACCCTTCGGTGGTGTCTCCGATTATGGTGAGGTACATCAGGGACCCGAGGAAGTGGAATTTTGCCAAGAAGCCTCTGCTTGTTGTGATGGATCCTCAAGGCAAAATTGTGCACAAGAATGCTGTTCACATGATGTGCATTTGGGGAAGCATTGCCTTCCCTTTCACTAGCCAGAAAGAGAGAATGCTTTGGGAAGAAGAAACATGGAGGATTGAGCTTTTGGCTGATGCCCTAGATCAAAACTTATATACTTGGATCACGGATAGGAAGTACATTTGTTTGTATGGCGGAGAAGACATCGAGTGGATCCGAAACTTCACAAAGGCAGCGAGAAATGTGGCTCGTGAAGCCGGGATTCAGTTGGAATTGCTTTATGTGGGGAAGAGCAAGGCTAAGGAGAAAGTGGTGAAGAACATCATCAGCATCATTCAAGCTGAGAAGCTAAGCCACACCCTTGAGGTGAACCTCATTTGGTTCTTCTGGATTCGTCTCGAGAGCATGTGGCAGTCCAAAGGACAAATCCTAAGCGAGCAAAGCAGAACCCAAACTGATCATTTGAAAAGCGATCGTATAATGCAGGGGATCATATCGATGCTGAGCTTTGGCTCGAGCGACCGCGGATGGGCAGTGATCGCCCTTGGCTCAGCAGACATGTCCAAGGCCAACGGGGAGCACATGTTGAGAAGCTTGAGGGAGTACAACAACTGGAAAATAAGGCCAACTGAAATAGGGTTTACACCGGCACTGAATGAATACCTAGAAGGGGTGCATAAACAGGCTCCACACCACTGCACTAATCTTATTTTGCCTGCAACCGGAATCATGCCTGAGACAGTTGCCTGCGCTGAGTGCGGCCGTCTCATGGAGAGGTTCTCCATGTTCCGCTGCTGCACCGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0008150 GO:0009888 GO:0010087 GO:0010088 GO:0032502 GO:0042802 GO:0042803 GO:0043621 GO:0046983 GO:0048856

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SEO_C SEO_N

Protein Analysis

745

Amino Acids

85.31

Weight (kDa)

6.14

Isoelectric Point (pI)

40.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SEO_N	PF14576	40 - 339	8.8e-69	Sieve element occlusion N-terminus
SEO_C	PF14577	504 - 743	8e-86	Sieve element occlusion C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002043)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g25290 FvH4_4g25290 FvH4_4g25290 FvH4_4g25310 FvH4_4g25320
malus_domestica	MD13G1105000.v1.1 MD13G1105100.v1.1 MD16G1104800.v1.1 MD16G1105000.v1.1
prunus_persica	Prupe.1G241400_v2.0.a1 Prupe.1G241500_v2.0.a1
pyrus_communis	pycom13g09110 pycom13g09120 pycom16g08950 pycom16g08960
rosa_chinensis	RchiOBHm_Chr4g0432561 RchiOBHm_Chr4g0432571 RchiOBHm_Chr4g0432611
rosa_laevigata	RLG00000006831 RLG00000006832 RLG00000006833
rosa_multiflora	Rmu_sc0003886.1_g000001 Rmu_sc0004612.1_g000014 Rmu_sc0027699.1_g000001
rosa_roxburghii	Rroxscaffold_5G00373990 Rroxscaffold_5G00374000 Rroxscaffold_5G00374030
rosa_rugosa	Rorug04G0260100 Rorug04G0260100 Rorug04G0260200
rosa_samantha	Rh4AG315100 Rh4AG315200 Rh4AG315300 Rh4BG322800 Rh4BG322900 Rh4BG323000 Rh4CG337900 Rh4CG338000 Rh4CG338100 Rh4DG318600 Rh4DG318700 Rh4DG318800
rosa_wichuraiana	Rw4G027380 Rw4G027390 Rw4G027420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	3 cut(s)	63, 87, 1953
AciI	CCGC	9 cut(s)	51, 305, 447, 626, 1623, 1951, 1953, 2187, 2218
AclWI	GGATC	7 cut(s)	925, 1421, 1434, 1598, 1636, 1649, 1925
AcoI	YGGCCR	2 cut(s)	52, 2188
AcsI	RAATTY	2 cut(s)	204, 1393
AcuI	CTGAAG	1 cut(s)	1058
AcyI	GRCGYC	1 cut(s)	1278
AfiI	CCNNNNNNNGG	7 cut(s)	1240, 1342, 1345, 1957, 2098, 2150, 2197
AflII	CTTAAG	1 cut(s)	596
AflIII	ACRYGT	2 cut(s)	1986, 2010
AhlI	ACTAGT	1 cut(s)	632
AjnI	CCWGG	2 cut(s)	546, 692
Alw21I	GWGCWC	2 cut(s)	1449, 2010
Alw26I	GTCTC	5 cut(s)	39, 1356, 1829, 2159, 2198
Alw44I	GTGCAC	1 cut(s)	1445
AlwI	GGATC	7 cut(s)	925, 1421, 1434, 1598, 1636, 1649, 1925
Ama87I	CYCGRG	4 cut(s)	274, 1381, 1826, 1942
AoxI	GGCC	8 cut(s)	52, 222, 327, 891, 1223, 1995, 2051, 2188
ApaLI	GTGCAC	1 cut(s)	1445
ApeKI	GCWGC	7 cut(s)	293, 421, 761, 818, 1661, 2220, 2223
ApoI	RAATTY	2 cut(s)	204, 1393
AspLEI	GCGC	3 cut(s)	65, 87, 2180
AspS9I	GGNCC	4 cut(s)	328, 696, 892, 1378
AsuC2I	CCSGG	3 cut(s)	275, 276, 1687
AsuHPI	GGTGA	4 cut(s)	911, 1375, 1753, 1807
AvaI	CYCGRG	4 cut(s)	274, 1381, 1826, 1942
AvaII	GGWCC	2 cut(s)	696, 1378
BaeGI	GKGCMC	1 cut(s)	1449
BaeI	ACNNNNGTAYC	2 cut(s)	331, 364
BamHI	GGATCC	2 cut(s)	1426, 1641
BanII	GRGCYC	1 cut(s)	743
BauI	CACGAG	2 cut(s)	608, 1677
BbsI	GAAGAC	1 cut(s)	1635
Bbv12I	GWGCWC	2 cut(s)	1449, 2010
BbvI	GCAGC	7 cut(s)	305, 408, 748, 805, 1673, 2207, 2210
BccI	CCATC	6 cut(s)	74, 177, 254, 524, 1417, 1950
BceAI	ACGGC	3 cut(s)	39, 64, 2175
BciT130I	CCWGG	2 cut(s)	548, 694
BclI	TGATCA	3 cut(s)	132, 1060, 1885
BcnI	CCSGG	3 cut(s)	275, 276, 1687
BcoDI	GTCTC	5 cut(s)	39, 1356, 1829, 2159, 2198
BcuI	ACTAGT	1 cut(s)	632
BfaI	CTAG	7 cut(s)	269, 461, 633, 1151, 1503, 1568, 2093
BfmI	CTRYAG	1 cut(s)	756
BfrI	CTTAAG	1 cut(s)	596
BglII	AGATCT	1 cut(s)	612
BisI	GCNGC	9 cut(s)	52, 294, 422, 762, 819, 1662, 2188, 2221, 2224
BlpI	GCTNAGC	4 cut(s)	552, 1778, 1931, 1978
BlsI	GCNGC	9 cut(s)	53, 295, 423, 763, 820, 1663, 2189, 2222, 2225
Bme1390I	CCNGG	5 cut(s)	275, 276, 548, 694, 1687
Bme18I	GGWCC	2 cut(s)	696, 1378
BmeT110I	CYCGRG	4 cut(s)	274, 1381, 1826, 1942
BmgT120I	GGNCC	4 cut(s)	328, 696, 892, 1378
BmiI	GGNNCC	7 cut(s)	545, 847, 1379, 1380, 1428, 1643, 2115
BmrFI	CCNGG	5 cut(s)	275, 276, 548, 694, 1687
BpiI	GAAGAC	1 cut(s)	1635
BplI	GAGNNNNNCTC	2 cut(s)	2192, 2224
Bpu10I	CCTNAGC	2 cut(s)	1728, 1859
Bpu1102I	GCTNAGC	4 cut(s)	552, 1778, 1931, 1978
BpuEI	CTTGAG	3 cut(s)	1416, 1811, 2044
BpuMI	CCSGG	3 cut(s)	275, 276, 1687
Bsa29I	ATCGAT	1 cut(s)	1925
BsaAI	YACGTR	1 cut(s)	862
BsaHI	GRCGYC	1 cut(s)	1278
BsaJI	CCNNGG	6 cut(s)	274, 507, 1382, 1951, 1972, 1992
BsaWI	WCCGGW	1 cut(s)	2150
BsaXI	ACNNNNNCTCC	6 cut(s)	270, 300, 1220, 1250, 1997, 2027
Bsc4I	CCNNNNNNNGG	7 cut(s)	1240, 1342, 1345, 1957, 2098, 2150, 2197
Bse118I	RCCGGY	1 cut(s)	2074
Bse1I	ACTGG	3 cut(s)	797, 1234, 2045
Bse3DI	GCAATG	2 cut(s)	456, 1485
BseBI	CCWGG	2 cut(s)	548, 694
BseCI	ATCGAT	1 cut(s)	1925
BseDI	CCNNGG	6 cut(s)	274, 507, 1382, 1951, 1972, 1992
BseGI	GGATG	6 cut(s)	169, 252, 493, 982, 1063, 1961
BseLI	CCNNNNNNNGG	7 cut(s)	1240, 1342, 1345, 1957, 2098, 2150, 2197
BseMI	GCAATG	2 cut(s)	456, 1485
BseMII	CTCAG	7 cut(s)	335, 566, 1761, 1922, 1992, 2154, 2172
BseNI	ACTGG	3 cut(s)	797, 1234, 2045
BseRI	GAGGAG	1 cut(s)	57
BseSI	GKGCMC	1 cut(s)	1449
BseX3I	CGGCCG	2 cut(s)	52, 2188
BseXI	GCAGC	7 cut(s)	305, 408, 748, 805, 1673, 2207, 2210
BsgI	GTGCAG	4 cut(s)	590, 1053, 2110, 2209
Bsh1236I	CGCG	3 cut(s)	63, 87, 1953
Bsh1285I	CGRYCG	4 cut(s)	55, 1903, 1951, 2191
BshFI	GGCC	8 cut(s)	54, 224, 329, 893, 1225, 1997, 2053, 2190
BshVI	ATCGAT	1 cut(s)	1925
BsiEI	CGRYCG	4 cut(s)	55, 1903, 1951, 2191
BsiHKAI	GWGCWC	2 cut(s)	1449, 2010
BsiHKCI	CYCGRG	4 cut(s)	274, 1381, 1826, 1942
BsiSI	CCGG	5 cut(s)	275, 1226, 1686, 2075, 2151
BslFI	GGGAC	3 cut(s)	257, 643, 1391
BslI	CCNNNNNNNGG	7 cut(s)	1240, 1342, 1345, 1957, 2098, 2150, 2197
BsmAI	GTCTC	5 cut(s)	39, 1356, 1829, 2159, 2198
BsmBI	CGTCTC	3 cut(s)	39, 1829, 2198
BsmFI	GGGAC	3 cut(s)	257, 643, 1391
BsmI	GAATGC	2 cut(s)	1459, 1524
BsnI	GGCC	8 cut(s)	54, 224, 329, 893, 1225, 1997, 2053, 2190
BsoBI	CYCGRG	4 cut(s)	274, 1381, 1826, 1942
Bsp1286I	GDGCHC	3 cut(s)	743, 1449, 2010
Bsp1720I	GCTNAGC	4 cut(s)	552, 1778, 1931, 1978
BspACI	CCGC	9 cut(s)	51, 305, 447, 626, 1623, 1951, 1953, 2187, 2218
BspANI	GGCC	8 cut(s)	54, 224, 329, 893, 1225, 1997, 2053, 2190
BspCNI	CTCAG	7 cut(s)	336, 565, 1762, 1923, 1991, 2155, 2173
BspDI	ATCGAT	1 cut(s)	1925
BspFNI	CGCG	3 cut(s)	63, 87, 1953
BspHI	TCATGA	1 cut(s)	163
BspLI	GGNNCC	7 cut(s)	545, 847, 1379, 1380, 1428, 1643, 2115
BspPI	GGATC	7 cut(s)	925, 1421, 1434, 1598, 1636, 1649, 1925
BspQI	GCTCTTC	1 cut(s)	1712
BspTI	CTTAAG	1 cut(s)	596
BsrDI	GCAATG	2 cut(s)	456, 1485
BsrFI	RCCGGY	1 cut(s)	2074
BsrI	ACTGG	3 cut(s)	797, 1234, 2045
BssAI	RCCGGY	1 cut(s)	2074
BssECI	CCNNGG	6 cut(s)	274, 507, 1382, 1951, 1972, 1992
BssNI	GRCGYC	1 cut(s)	1278
BssSI	CACGAG	2 cut(s)	608, 1677
BssT1I	CCWWGG	3 cut(s)	507, 1972, 1992
Bst2BI	CACGAG	2 cut(s)	608, 1677
Bst2UI	CCWGG	2 cut(s)	548, 694
Bst4CI	ACNGT	3 cut(s)	382, 1330, 2170
Bst6I	CTCTTC	3 cut(s)	921, 1088, 1712
BstACI	GRCGYC	1 cut(s)	1278
BstAFI	CTTAAG	1 cut(s)	596
BstBAI	YACGTR	1 cut(s)	862
BstC8I	GCNNGC	7 cut(s)	504, 891, 997, 1068, 1865, 2145, 2176
BstDSI	CCRYGG	1 cut(s)	1951
BstF5I	GGATG	6 cut(s)	169, 252, 493, 982, 1063, 1961
BstFNI	CGCG	3 cut(s)	63, 87, 1953
BstHHI	GCGC	3 cut(s)	65, 87, 2180
BstMAI	GTCTC	5 cut(s)	39, 1356, 1829, 2159, 2198
BstMCI	CGRYCG	4 cut(s)	55, 1903, 1951, 2191
BstNI	CCWGG	2 cut(s)	548, 694
BstNSI	RCATGY	4 cut(s)	305, 1837, 1990, 2014
BstSCI	CCNGG	5 cut(s)	273, 274, 546, 692, 1685
BstSFI	CTRYAG	1 cut(s)	756
BstSLI	GKGCMC	1 cut(s)	1449
BstUI	CGCG	3 cut(s)	63, 87, 1953
BstV1I	GCAGC	7 cut(s)	305, 408, 748, 805, 1673, 2207, 2210
BstV2I	GAAGAC	1 cut(s)	1635
BstX2I	RGATCY	4 cut(s)	612, 930, 1426, 1641
BstYI	RGATCY	4 cut(s)	612, 930, 1426, 1641
BstZI	CGGCCG	2 cut(s)	52, 2188
Bsu15I	ATCGAT	1 cut(s)	1925
BsuRI	GGCC	8 cut(s)	54, 224, 329, 893, 1225, 1997, 2053, 2190
BsuTUI	ATCGAT	1 cut(s)	1925
BtgI	CCRYGG	1 cut(s)	1951
BtgZI	GCGATG	1 cut(s)	870
BtsCI	GGATG	6 cut(s)	169, 252, 493, 982, 1063, 1961
BtsI	GCAGTG	3 cut(s)	765, 1968, 2122
BtsIMutI	CAGTG	5 cut(s)	5, 765, 1968, 2078, 2122
Cac8I	GCNNGC	7 cut(s)	504, 891, 997, 1068, 1865, 2145, 2176
CciI	TCATGA	1 cut(s)	163
CfoI	GCGC	3 cut(s)	65, 87, 2180
Cfr10I	RCCGGY	1 cut(s)	2074
Cfr13I	GGNCC	4 cut(s)	328, 696, 892, 1378
Cfr42I	CCGCGG	1 cut(s)	1954
Cfr9I	CCCGGG	1 cut(s)	274
ClaI	ATCGAT	1 cut(s)	1925
CseI	GACGC	1 cut(s)	1286
EaeI	YGGCCR	2 cut(s)	52, 2188
EagI	CGGCCG	2 cut(s)	52, 2188
Eam1104I	CTCTTC	3 cut(s)	921, 1088, 1712
EarI	CTCTTC	3 cut(s)	921, 1088, 1712
EciI	GGCGGA	1 cut(s)	1638
EclXI	CGGCCG	2 cut(s)	52, 2188
Eco130I	CCWWGG	3 cut(s)	507, 1972, 1992
Eco24I	GRGCYC	1 cut(s)	743
Eco47I	GGWCC	2 cut(s)	696, 1378
Eco52I	CGGCCG	2 cut(s)	52, 2188
Eco57I	CTGAAG	1 cut(s)	1058
Eco88I	CYCGRG	4 cut(s)	274, 1381, 1826, 1942
EcoO109I	RGGNCCY	2 cut(s)	328, 1378
EcoRII	CCWGG	2 cut(s)	546, 692
EcoT14I	CCWWGG	3 cut(s)	507, 1972, 1992
EcoT22I	ATGCAT	2 cut(s)	130, 658
EcoT38I	GRGCYC	1 cut(s)	743
ErhI	CCWWGG	3 cut(s)	507, 1972, 1992
Esp3I	CGTCTC	3 cut(s)	39, 1829, 2198
FaqI	GGGAC	3 cut(s)	257, 643, 1391
FauI	CCCGC	1 cut(s)	619
FbaI	TGATCA	3 cut(s)	132, 1060, 1885
Fnu4HI	GCNGC	9 cut(s)	52, 294, 422, 762, 819, 1662, 2188, 2221, 2224
FokI	GGATG	6 cut(s)	156, 239, 500, 989, 1070, 1968
FriOI	GRGCYC	1 cut(s)	743
Fsp4HI	GCNGC	9 cut(s)	52, 294, 422, 762, 819, 1662, 2188, 2221, 2224
FspBI	CTAG	7 cut(s)	269, 461, 633, 1151, 1503, 1568, 2093
GlaI	GCGC	3 cut(s)	64, 86, 2179
GluI	GCNGC	9 cut(s)	52, 294, 422, 762, 819, 1662, 2188, 2221, 2224
HaeIII	GGCC	8 cut(s)	54, 224, 329, 893, 1225, 1997, 2053, 2190
HapII	CCGG	5 cut(s)	275, 1226, 1686, 2075, 2151
HgaI	GACGC	1 cut(s)	1286
HhaI	GCGC	3 cut(s)	65, 87, 2180
Hin1I	GRCGYC	1 cut(s)	1278
Hin6I	GCGC	3 cut(s)	63, 85, 2178
HinP1I	GCGC	3 cut(s)	63, 85, 2178
HincII	GTYRAC	2 cut(s)	31, 1102
HindII	GTYRAC	2 cut(s)	31, 1102
HindIII	AAGCTT	1 cut(s)	2020
HinfI	GANTC	5 cut(s)	308, 718, 1690, 1819, 2154
HpaII	CCGG	5 cut(s)	275, 1226, 1686, 2075, 2151
HphI	GGTGA	4 cut(s)	911, 1375, 1753, 1807
Hpy188I	TCNGA	4 cut(s)	850, 1038, 1356, 1646
Hpy188III	TCNNGA	9 cut(s)	77, 164, 241, 958, 1297, 1679, 1816, 1826, 1828
Hpy99I	CGWCG	1 cut(s)	92
HpyAV	CCTTC	6 cut(s)	195, 319, 1033, 1350, 1501, 2090
HpyCH4III	ACNGT	3 cut(s)	382, 1330, 2170
HpyCH4IV	ACGT	3 cut(s)	622, 861, 1155
HpySE526I	ACGT	3 cut(s)	622, 861, 1155
Hsp92I	GRCGYC	1 cut(s)	1278
HspAI	GCGC	3 cut(s)	63, 85, 2178
KflI	GGGWCCC	1 cut(s)	1378
Ksp22I	TGATCA	3 cut(s)	132, 1060, 1885
KspI	CCGCGG	1 cut(s)	1954
LguI	GCTCTTC	1 cut(s)	1712
LmnI	GCTCC	8 cut(s)	70, 278, 316, 512, 549, 851, 2005, 2119
Lsp1109I	GCAGC	7 cut(s)	305, 408, 748, 805, 1673, 2207, 2210
MaeI	CTAG	7 cut(s)	269, 461, 633, 1151, 1503, 1568, 2093
MaeII	ACGT	3 cut(s)	622, 861, 1155
MaeIII	GTNAC	3 cut(s)	196, 688, 862
MfeI	CAATTG	1 cut(s)	990
MflI	RGATCY	4 cut(s)	612, 930, 1426, 1641
MhlI	GDGCHC	3 cut(s)	743, 1449, 2010
MmeI	TCCRAC	3 cut(s)	568, 1210, 1677
Mph1103I	ATGCAT	2 cut(s)	130, 658
MseI	TTAA	2 cut(s)	407, 597
MslI	CAYNNNNRTG	3 cut(s)	1109, 1452, 1925
MspA1I	CMGCKG	5 cut(s)	116, 761, 785, 1953, 2220
MspCI	CTTAAG	1 cut(s)	596
MspI	CCGG	5 cut(s)	275, 1226, 1686, 2075, 2151
MspR9I	CCNGG	5 cut(s)	275, 276, 548, 694, 1687
MunI	CAATTG	1 cut(s)	990
Mva1269I	GAATGC	2 cut(s)	1459, 1524
MvaI	CCWGG	2 cut(s)	548, 694
MvnI	CGCG	3 cut(s)	63, 87, 1953
NciI	CCSGG	3 cut(s)	275, 276, 1687
NlaIV	GGNNCC	7 cut(s)	545, 847, 1379, 1380, 1428, 1643, 2115
NmuCI	GTSAC	3 cut(s)	196, 688, 862
NsiI	ATGCAT	2 cut(s)	130, 658
NspI	RCATGY	4 cut(s)	305, 1837, 1990, 2014
PaeR7I	CTCGAG	2 cut(s)	1826, 1942
PagI	TCATGA	1 cut(s)	163
PciI	ACATGT	2 cut(s)	1986, 2010
PciSI	GCTCTTC	1 cut(s)	1712
PcsI	WCGNNNNNNNCGW	2 cut(s)	363, 1161
PctI	GAATGC	2 cut(s)	1459, 1524
PfeI	GAWTC	5 cut(s)	308, 718, 1690, 1819, 2154
PflFI	GACNNNGTC	1 cut(s)	96
PkrI	GCNGC	9 cut(s)	53, 295, 423, 763, 820, 1663, 2189, 2222, 2225
Ple19I	CGATCG	1 cut(s)	1903
Ppu21I	YACGTR	1 cut(s)	862
PpuMI	RGGWCCY	1 cut(s)	1378
PscI	ACATGT	2 cut(s)	1986, 2010
Psp5II	RGGWCCY	1 cut(s)	1378
Psp6I	CCWGG	2 cut(s)	546, 692
PspGI	CCWGG	2 cut(s)	546, 692
PspN4I	GGNNCC	7 cut(s)	545, 847, 1379, 1380, 1428, 1643, 2115
PspPI	GGNCC	4 cut(s)	328, 696, 892, 1378
PspPPI	RGGWCCY	1 cut(s)	1378
PspXI	VCTCGAGB	1 cut(s)	1942
PsrI	GAACNNNNNNTAC	2 cut(s)	1316, 1348
PsuI	RGATCY	4 cut(s)	612, 930, 1426, 1641
PsyI	GACNNNGTC	1 cut(s)	96
PvuI	CGATCG	1 cut(s)	1903
PvuII	CAGCTG	3 cut(s)	116, 761, 785
RseI	CAYNNNNRTG	3 cut(s)	1109, 1452, 1925
SacII	CCGCGG	1 cut(s)	1954
SapI	GCTCTTC	1 cut(s)	1712
SaqAI	TTAA	2 cut(s)	407, 597
SatI	GCNGC	9 cut(s)	52, 294, 422, 762, 819, 1662, 2188, 2221, 2224
Sau96I	GGNCC	4 cut(s)	328, 696, 892, 1378
ScrFI	CCNGG	5 cut(s)	275, 276, 548, 694, 1687
SduI	GDGCHC	3 cut(s)	743, 1449, 2010
SfcI	CTRYAG	1 cut(s)	756
Sfr274I	CTCGAG	2 cut(s)	1826, 1942
Sfr303I	CCGCGG	1 cut(s)	1954
SgrBI	CCGCGG	1 cut(s)	1954
SinI	GGWCC	2 cut(s)	696, 1378
SlaI	CTCGAG	2 cut(s)	1826, 1942
SmaI	CCCGGG	1 cut(s)	276
SmiMI	CAYNNNNRTG	3 cut(s)	1109, 1452, 1925
SmlI	CTYRAG	6 cut(s)	596, 1431, 1790, 1826, 1942, 2023
SmoI	CTYRAG	6 cut(s)	596, 1431, 1790, 1826, 1942, 2023
SpeI	ACTAGT	1 cut(s)	632
SsiI	CCGC	9 cut(s)	51, 305, 447, 626, 1623, 1951, 1953, 2187, 2218
SspMI	CTAG	7 cut(s)	269, 461, 633, 1151, 1503, 1568, 2093
StyD4I	CCNGG	5 cut(s)	273, 274, 546, 692, 1685
StyI	CCWWGG	3 cut(s)	507, 1972, 1992
TaaI	ACNGT	3 cut(s)	382, 1330, 2170
TaiI	ACGT	3 cut(s)	625, 864, 1158
TatI	WGTACW	4 cut(s)	1173, 1194, 1605, 2031
TauI	GCSGC	2 cut(s)	54, 2190
TfiI	GAWTC	5 cut(s)	308, 718, 1690, 1819, 2154
Tru1I	TTAA	2 cut(s)	407, 597
Tru9I	TTAA	2 cut(s)	407, 597
TscAI	CASTG	5 cut(s)	12, 772, 1968, 2085, 2129
TseFI	GTSAC	3 cut(s)	196, 688, 862
TseI	GCWGC	7 cut(s)	293, 421, 761, 818, 1661, 2220, 2223
Tsp45I	GTSAC	3 cut(s)	196, 688, 862
TspDTI	ATGAA	3 cut(s)	504, 1129, 2100
TspGWI	ACGGA	2 cut(s)	105, 1610
TspMI	CCCGGG	1 cut(s)	274
TspRI	CASTG	5 cut(s)	12, 772, 1968, 2085, 2129
Tth111I	GACNNNGTC	1 cut(s)	96
Vha464I	CTTAAG	1 cut(s)	596
VneI	GTGCAC	1 cut(s)	1445
VpaK11BI	GGWCC	2 cut(s)	696, 1378
XapI	RAATTY	2 cut(s)	204, 1393
XceI	RCATGY	4 cut(s)	305, 1837, 1990, 2014
XhoI	CTCGAG	2 cut(s)	1826, 1942
XmaI	CCCGGG	1 cut(s)	274
XspI	CTAG	7 cut(s)	269, 461, 633, 1151, 1503, 1568, 2093
Zsp2I	ATGCAT	2 cut(s)	130, 658

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.