Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002043

Physical Location & Seq

Genomic Coordinates Forward (+)

7370980 .. 7374176

Gene Structure 3197 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1105000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2232 bp

ATGGCCCTGGTACCTTACAATAAGGCTGTCAACCCAACAAGCAGAGGAGCCGGTCATAATCGCCCTCCTCTGCTTAAACAAGGGCGTGACGGACATCGTCAATTTTCGACAACAGCAGATGACAGTGCATTGGTCAACCAAATTATGCTTACCGATAAGTCTCATGACCATCCCTACGAAGTGCCTCCTCTTGCAGTCACGCAAATTCTTCAAACCATTGAGGCCATTCTTTCGCGCGTCACCAAACCTGACATTCATGATGGCATTCTAGTCCCGGGAGCATTAGCGCCGGCAGCTACACACGCGGAGCATGAGAAGGCTCTCCATGCTAACCTGAGTACCCTCCACGACAGCGTTGATGTTCCACACAGCGTGTTCAATGCAATTCACTCCGCGTTAATGTGCACATTTGCGCAAGGGGAAGATGCAAACAAGACAACAATGGACATACTAGACATTGTGCAACACTACGATTGGGATGAAAAGGCGGTGCTTGCCTTGGGAGCATTTGCGATCAAAGATGGCGAGTTTTGGCTCGTGTCTCAGCAATACACCACCAACCCACTCGCCAGAGCGGTTGGACAGCTTAAGCAACTGCAAGAGATCTTGGAACGTGCAGGGACTATTTTGAAACCGAAGTTCGATGCATACAACAATCTGGTTGGGGCCATTTTCAAAGTGACCAAGACCATTGTTCGCTTACAGGAGATTCACAACGATCCCCATTTGAGCCCCGAAATCAAAGCTACAGCTGCCACTGCTCAGATCCCCACAGCTGTTTACTGGACAATTCGGGCTATTGTAGTTGCCGCATCACAACTTGTGGGAATCACAAGCTCCGAGCCAGAGTACATGACAGAGGCGTGGGAACTATCATCACTGGCCCATAAGCTCGAAAACATATTCAAACACCTTGAAGAGATCCTCGACGGATTATATCAACTCATCCAGAAGAACAAAGAAGAGGAAGCTTTCAACGCAATTGCGCGCATCTTGGAAACACCGCACATGGATAACTCGAAGCCTCTGAGGGTTTTGTTTTACAAGGATGATCAGCCTGCACTATATGATGGCTACAACAAGAAGAGGGTTGACATTGATGTGCTAAAGAGGAAAGTAGTGATTCTGTTCATCACAGACCTAAACGTTGTCCACGAAAATGAGAACACAATTGTCCAACAAATGTACTTGGAAAAGGGACACAACCCTACAAGGCCGGAGAGCCAATACGAGGTTGTGTGGGTTCCAATTACGGATGAATGGACTGAAGTCAAGTACCAGCAATTCGAGAGTCTAAGAAATAGCATGCAATGGTACACCGTGTTCCACCCTTCTGTAGTGTCTCCGATTGTGGTCAGGTACATCAGGGACCCGAGGAAGTGGAATTTTGCCAAGAAGCCTCTGCTTGTTGTGATGGATCCTCAAGGCAAAATTGTGCACCAGAATGCTGTTCACATGATGTGCATTTGGGGGAGCATTGCCTTCCCCTTCACTAGCCAGAGAGAGAGATTGCTTTGGGAAGAAGAAACCTGGAGGATCGAGCTTTTGGCTGATGCCATCCATCCAAACTTAACTACTTGGATTACGGAGGGGAAGTACATTTGCTTGTATGGCGGAGAAGACATCGAGTGGATCCGAAAGTTCACAAAGGCTGCGAGAAACGTGGCTAGTGACGCCGGCATTCAGTTGGAAATGCTATATGTGGGGCGGAGCAAGGCTAAGGAGAACGTAATGAATAACATCATAAAAATCATCCAAGCTGAGAAGCTAAGCCACACCCTTGACTGGAACCTCATTTGGTTCTTCTGGATTCGTCTCGAGAGCATGTGGCAATCCAAAGGACAACTTCTAAGTGAGCAAGGCAAAACCCAATCCCGAACTGATCCTTTGAAAAGCGATCCTATGATGCAGGGGATCATATCGCTGCTGAGCTTTGGCTCGAGCGACCGCGGATGGGCAGTGATCAGCCTAGGCTCCGCTGCCATGTCCAAGGCAAATGGAGAGCACATGTTGAAAAGCTTGAGCGAGTTTAACGACTGGAAAAATAGGTCATCTGAAGTAGGGTTCACGCCGGCCCTGAATGAACACCTAGAAGGGGTGCACAGACAAGCTCCACACCACTGCACCAGTCTTATTTTGCCCGCAACCGGAATCATGCCCGAGACGGTGGCGTGCGCTGAGTGCGGCCGTGTCATGGAGAGGTTCTCCATGTTCCGCTGCTGCCTCGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0008150 GO:0009888 GO:0010087 GO:0010088 GO:0032502 GO:0042802 GO:0042803 GO:0043621 GO:0046983 GO:0048856

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SEO_C SEO_N

Protein Analysis

744

Amino Acids

84.26

Weight (kDa)

6.14

Isoelectric Point (pI)

39.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SEO_N	PF14576	40 - 336	7.3e-67	Sieve element occlusion N-terminus
SEO_C	PF14577	501 - 741	9.6e-89	Sieve element occlusion C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002043)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g25290 FvH4_4g25290 FvH4_4g25290 FvH4_4g25310 FvH4_4g25320
malus_domestica	MD13G1105000.v1.1 MD13G1105100.v1.1 MD16G1104800.v1.1 MD16G1105000.v1.1
prunus_persica	Prupe.1G241400_v2.0.a1 Prupe.1G241500_v2.0.a1
pyrus_communis	pycom13g09110 pycom13g09120 pycom16g08950 pycom16g08960
rosa_chinensis	RchiOBHm_Chr4g0432561 RchiOBHm_Chr4g0432571 RchiOBHm_Chr4g0432611
rosa_laevigata	RLG00000006831 RLG00000006832 RLG00000006833
rosa_multiflora	Rmu_sc0003886.1_g000001 Rmu_sc0004612.1_g000014 Rmu_sc0027699.1_g000001
rosa_roxburghii	Rroxscaffold_5G00373990 Rroxscaffold_5G00374000 Rroxscaffold_5G00374030
rosa_rugosa	Rorug04G0260100 Rorug04G0260100 Rorug04G0260200
rosa_samantha	Rh4AG315100 Rh4AG315200 Rh4AG315300 Rh4BG322800 Rh4BG322900 Rh4BG323000 Rh4CG337900 Rh4CG338000 Rh4CG338100 Rh4DG318600 Rh4DG318700 Rh4DG318800
rosa_wichuraiana	Rw4G027380 Rw4G027390 Rw4G027420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	414
Acc65I	GGTACC	1 cut(s)	10
AccB1I	GGYRCC	1 cut(s)	10
AccBSI	CCGCTC	1 cut(s)	575
AccII	CGCG	6 cut(s)	235, 237, 305, 395, 988, 1950
AclI	AACGTT	1 cut(s)	1146
AcoI	YGGCCR	1 cut(s)	2185
AcsI	RAATTY	2 cut(s)	204, 1384
AcuI	CTGAAG	2 cut(s)	1287, 2076
AcyI	GRCGYC	1 cut(s)	1674
AdeI	CACNNNGTG	1 cut(s)	373
AfaI	GTAC	8 cut(s)	12, 340, 851, 1187, 1277, 1316, 1361, 1598
AfiI	CCNNNNNNNGG	6 cut(s)	1231, 1785, 1954, 2095, 2147, 2194
AflII	CTTAAG	1 cut(s)	587
AflIII	ACRYGT	1 cut(s)	2007
AgsI	TTSAA	9 cut(s)	212, 379, 631, 676, 907, 917, 976, 1891, 2014
AjnI	CCWGG	2 cut(s)	6, 1529
Alw21I	GWGCWC	4 cut(s)	407, 1440, 2007, 2103
Alw26I	GTCTC	5 cut(s)	165, 546, 1347, 1820, 2156
Alw44I	GTGCAC	3 cut(s)	403, 1436, 2099
Ama87I	CYCGRG	5 cut(s)	274, 1372, 1817, 1939, 2159
AoxI	GGCC	7 cut(s)	3, 222, 666, 882, 1214, 2073, 2185
ApaLI	GTGCAC	3 cut(s)	403, 1436, 2099
ApeKI	GCWGC	7 cut(s)	293, 752, 1652, 1924, 1979, 2217, 2220
ApoI	RAATTY	2 cut(s)	204, 1384
Asp718I	GGTACC	1 cut(s)	10
AspA2I	CCTAGG	1 cut(s)	1969
AspLEI	GCGC	6 cut(s)	237, 289, 415, 988, 990, 2177
AspS9I	GGNCC	5 cut(s)	4, 666, 883, 1369, 2074
AsuC2I	CCSGG	2 cut(s)	275, 276
AsuHPI	GGTGA	1 cut(s)	232
AvaI	CYCGRG	5 cut(s)	274, 1372, 1817, 1939, 2159
AvaII	GGWCC	1 cut(s)	1369
AvrII	CCTAGG	1 cut(s)	1969
BaeGI	GKGCMC	3 cut(s)	407, 1440, 2103
BaeI	ACNNNNGTAYC	2 cut(s)	322, 355
BamHI	GGATCC	2 cut(s)	1417, 1632
BanI	GGYRCC	1 cut(s)	10
BanII	GRGCYC	1 cut(s)	734
BauI	CACGAG	1 cut(s)	536
BbsI	GAAGAC	1 cut(s)	1626
Bbv12I	GWGCWC	4 cut(s)	407, 1440, 2007, 2103
BbvI	GCAGC	7 cut(s)	305, 739, 1639, 1911, 1966, 2204, 2207
BccI	CCATC	8 cut(s)	177, 254, 515, 1064, 1408, 1565, 1569, 1947
BceAI	ACGGC	1 cut(s)	2172
BciT130I	CCWGG	2 cut(s)	8, 1531
BclI	TGATCA	2 cut(s)	1051, 1962
BcnI	CCSGG	2 cut(s)	275, 276
BcoDI	GTCTC	5 cut(s)	165, 546, 1347, 1820, 2156
BfaI	CTAG	6 cut(s)	269, 452, 1494, 1668, 1970, 2090
BfmI	CTRYAG	2 cut(s)	747, 1335
BfoI	RGCGCY	1 cut(s)	290
BfrI	CTTAAG	1 cut(s)	587
BglII	AGATCT	1 cut(s)	603
BisI	GCNGC	9 cut(s)	294, 753, 810, 1653, 1925, 1980, 2185, 2218, 2221
BlnI	CCTAGG	1 cut(s)	1969
BlpI	GCTNAGC	2 cut(s)	1769, 1928
BlsI	GCNGC	9 cut(s)	295, 754, 811, 1654, 1926, 1981, 2186, 2219, 2222
Bme1390I	CCNGG	4 cut(s)	8, 275, 276, 1531
Bme18I	GGWCC	1 cut(s)	1369
BmeT110I	CYCGRG	5 cut(s)	274, 1372, 1817, 1939, 2159
BmgT120I	GGNCC	5 cut(s)	4, 666, 883, 1369, 2074
BmrFI	CCNGG	4 cut(s)	8, 275, 276, 1531
BmsI	GCATC	6 cut(s)	415, 634, 821, 999, 1543, 1896
BoxI	GACNNNNGTC	1 cut(s)	1268
BpiI	GAAGAC	1 cut(s)	1626
BplI	GAGNNNNNCTC	2 cut(s)	2189, 2221
BpmI	CTGGAG	1 cut(s)	1552
Bpu10I	CCTNAGC	1 cut(s)	1719
Bpu1102I	GCTNAGC	2 cut(s)	1769, 1928
BpuEI	CTTGAG	2 cut(s)	1407, 2041
BpuMI	CCSGG	2 cut(s)	275, 276
BsaHI	GRCGYC	1 cut(s)	1674
BsaJI	CCNNGG	7 cut(s)	6, 274, 498, 1373, 1948, 1969, 1989
BsaWI	WCCGGW	1 cut(s)	2147
BsaXI	ACNNNNNCTCC	2 cut(s)	1580, 1610
Bsc4I	CCNNNNNNNGG	6 cut(s)	1231, 1785, 1954, 2095, 2147, 2194
Bse118I	RCCGGY	4 cut(s)	50, 289, 1676, 2071
Bse1I	ACTGG	5 cut(s)	788, 885, 1790, 2042, 2127
Bse3DI	GCAATG	2 cut(s)	1316, 1476
BseBI	CCWGG	2 cut(s)	8, 1531
BseDI	CCNNGG	7 cut(s)	6, 274, 498, 1373, 1948, 1969, 1989
BseGI	GGATG	9 cut(s)	169, 484, 945, 1054, 1261, 1557, 1561, 1752, 1958
BseLI	CCNNNNNNNGG	6 cut(s)	1231, 1785, 1954, 2095, 2147, 2194
BseMI	GCAATG	2 cut(s)	1316, 1476
BseMII	CTCAG	7 cut(s)	326, 557, 776, 1019, 1752, 1919, 2169
BseNI	ACTGG	5 cut(s)	788, 885, 1790, 2042, 2127
BsePI	GCGCGC	1 cut(s)	986
BseRI	GAGGAG	3 cut(s)	57, 60, 177
BseSI	GKGCMC	3 cut(s)	407, 1440, 2103
BseX3I	CGGCCG	1 cut(s)	2185
BseXI	GCAGC	7 cut(s)	305, 739, 1639, 1911, 1966, 2204, 2207
BsgI	GTGCAG	3 cut(s)	636, 1044, 2107
Bsh1236I	CGCG	6 cut(s)	235, 237, 305, 395, 988, 1950
Bsh1285I	CGRYCG	2 cut(s)	1948, 2188
BshFI	GGCC	7 cut(s)	5, 224, 668, 884, 1216, 2075, 2187
BshNI	GGYRCC	1 cut(s)	10
BsiEI	CGRYCG	2 cut(s)	1948, 2188
BsiHKAI	GWGCWC	4 cut(s)	407, 1440, 2007, 2103
BsiHKCI	CYCGRG	5 cut(s)	274, 1372, 1817, 1939, 2159
BsiSI	CCGG	7 cut(s)	51, 275, 290, 1217, 1677, 2072, 2148
BslFI	GGGAC	4 cut(s)	257, 634, 1212, 1382
BslI	CCNNNNNNNGG	6 cut(s)	1231, 1785, 1954, 2095, 2147, 2194
BsmAI	GTCTC	5 cut(s)	165, 546, 1347, 1820, 2156
BsmBI	CGTCTC	2 cut(s)	1820, 2156
BsmFI	GGGAC	4 cut(s)	257, 634, 1212, 1382
BsmI	GAATGC	3 cut(s)	264, 1450, 1680
BsnI	GGCC	7 cut(s)	5, 224, 668, 884, 1216, 2075, 2187
BsoBI	CYCGRG	5 cut(s)	274, 1372, 1817, 1939, 2159
Bsp1286I	GDGCHC	5 cut(s)	407, 734, 1440, 2007, 2103
Bsp1720I	GCTNAGC	2 cut(s)	1769, 1928
BspANI	GGCC	7 cut(s)	5, 224, 668, 884, 1216, 2075, 2187
BspCNI	CTCAG	7 cut(s)	327, 556, 775, 1020, 1753, 1920, 2170
BspFNI	CGCG	6 cut(s)	235, 237, 305, 395, 988, 1950
BspHI	TCATGA	2 cut(s)	163, 256
BspT107I	GGYRCC	1 cut(s)	10
BspTI	CTTAAG	1 cut(s)	587
BsrBI	CCGCTC	1 cut(s)	575
BsrDI	GCAATG	2 cut(s)	1316, 1476
BsrFI	RCCGGY	4 cut(s)	50, 289, 1676, 2071
BsrI	ACTGG	5 cut(s)	788, 885, 1790, 2042, 2127
BssAI	RCCGGY	4 cut(s)	50, 289, 1676, 2071
BssECI	CCNNGG	7 cut(s)	6, 274, 498, 1373, 1948, 1969, 1989
BssHII	GCGCGC	1 cut(s)	986
BssNI	GRCGYC	1 cut(s)	1674
BssSI	CACGAG	1 cut(s)	536
BssT1I	CCWWGG	3 cut(s)	498, 1969, 1989
Bst2BI	CACGAG	1 cut(s)	536
Bst2UI	CCWGG	2 cut(s)	8, 1531
Bst4CI	ACNGT	3 cut(s)	125, 1321, 2167
Bst6I	CTCTTC	3 cut(s)	912, 957, 1079
BstACI	GRCGYC	1 cut(s)	1674
BstAFI	CTTAAG	1 cut(s)	587
BstC8I	GCNNGC	9 cut(s)	291, 495, 988, 1059, 1307, 1678, 2073, 2142, 2173
BstDSI	CCRYGG	1 cut(s)	1948
BstF5I	GGATG	9 cut(s)	169, 484, 945, 1054, 1261, 1557, 1561, 1752, 1958
BstFNI	CGCG	6 cut(s)	235, 237, 305, 395, 988, 1950
BstH2I	RGCGCY	1 cut(s)	290
BstHHI	GCGC	6 cut(s)	237, 289, 415, 988, 990, 2177
BstMAI	GTCTC	5 cut(s)	165, 546, 1347, 1820, 2156
BstMCI	CGRYCG	2 cut(s)	1948, 2188
BstNI	CCWGG	2 cut(s)	8, 1531
BstNSI	RCATGY	3 cut(s)	1309, 1828, 2011
BstPAI	GACNNNNGTC	1 cut(s)	1268
BstSCI	CCNGG	4 cut(s)	6, 273, 274, 1529
BstSFI	CTRYAG	2 cut(s)	747, 1335
BstSLI	GKGCMC	3 cut(s)	407, 1440, 2103
BstUI	CGCG	6 cut(s)	235, 237, 305, 395, 988, 1950
BstV1I	GCAGC	7 cut(s)	305, 739, 1639, 1911, 1966, 2204, 2207
BstV2I	GAAGAC	1 cut(s)	1626
BstX2I	RGATCY	5 cut(s)	603, 765, 921, 1417, 1632
BstYI	RGATCY	5 cut(s)	603, 765, 921, 1417, 1632
BstZI	CGGCCG	1 cut(s)	2185
BsuRI	GGCC	7 cut(s)	5, 224, 668, 884, 1216, 2075, 2187
BtgI	CCRYGG	1 cut(s)	1948
BtsCI	GGATG	9 cut(s)	169, 484, 945, 1054, 1261, 1557, 1561, 1752, 1958
BtsI	GCAGTG	3 cut(s)	756, 1965, 2119
BtsIMutI	CAGTG	5 cut(s)	130, 756, 878, 1965, 2119
Cac8I	GCNNGC	9 cut(s)	291, 495, 988, 1059, 1307, 1678, 2073, 2142, 2173
CciI	TCATGA	2 cut(s)	163, 256
CfoI	GCGC	6 cut(s)	237, 289, 415, 988, 990, 2177
Cfr10I	RCCGGY	4 cut(s)	50, 289, 1676, 2071
Cfr13I	GGNCC	5 cut(s)	4, 666, 883, 1369, 2074
Cfr42I	CCGCGG	1 cut(s)	1951
Cfr9I	CCCGGG	1 cut(s)	274
CseI	GACGC	2 cut(s)	226, 1682
Csp6I	GTAC	8 cut(s)	11, 339, 850, 1186, 1276, 1315, 1360, 1597
CviQI	GTAC	8 cut(s)	11, 339, 850, 1186, 1276, 1315, 1360, 1597
DraIII	CACNNNGTG	1 cut(s)	373
EaeI	YGGCCR	1 cut(s)	2185
EagI	CGGCCG	1 cut(s)	2185
Eam1104I	CTCTTC	3 cut(s)	912, 957, 1079
EarI	CTCTTC	3 cut(s)	912, 957, 1079
EciI	GGCGGA	2 cut(s)	1629, 1723
EclXI	CGGCCG	1 cut(s)	2185
Eco130I	CCWWGG	3 cut(s)	498, 1969, 1989
Eco24I	GRGCYC	1 cut(s)	734
Eco47I	GGWCC	1 cut(s)	1369
Eco52I	CGGCCG	1 cut(s)	2185
Eco57I	CTGAAG	2 cut(s)	1287, 2076
Eco88I	CYCGRG	5 cut(s)	274, 1372, 1817, 1939, 2159
EcoO109I	RGGNCCY	1 cut(s)	1369
EcoRII	CCWGG	2 cut(s)	6, 1529
EcoT14I	CCWWGG	3 cut(s)	498, 1969, 1989
EcoT22I	ATGCAT	1 cut(s)	649
EcoT38I	GRGCYC	1 cut(s)	734
ErhI	CCWWGG	3 cut(s)	498, 1969, 1989
Esp3I	CGTCTC	2 cut(s)	1820, 2156
FalI	AAGNNNNNCTT	2 cut(s)	1830, 1862
FaqI	GGGAC	4 cut(s)	257, 634, 1212, 1382
FauI	CCCGC	1 cut(s)	2149
FbaI	TGATCA	2 cut(s)	1051, 1962
Fnu4HI	GCNGC	9 cut(s)	294, 753, 810, 1653, 1925, 1980, 2185, 2218, 2221
FokI	GGATG	9 cut(s)	156, 491, 932, 1061, 1268, 1544, 1548, 1739, 1965
FriOI	GRGCYC	1 cut(s)	734
Fsp4HI	GCNGC	9 cut(s)	294, 753, 810, 1653, 1925, 1980, 2185, 2218, 2221
FspBI	CTAG	6 cut(s)	269, 452, 1494, 1668, 1970, 2090
FspI	TGCGCA	1 cut(s)	414
GlaI	GCGC	6 cut(s)	236, 288, 414, 987, 989, 2176
GluI	GCNGC	9 cut(s)	294, 753, 810, 1653, 1925, 1980, 2185, 2218, 2221
GsuI	CTGGAG	1 cut(s)	1552
HaeII	RGCGCY	1 cut(s)	290
HaeIII	GGCC	7 cut(s)	5, 224, 668, 884, 1216, 2075, 2187
HapII	CCGG	7 cut(s)	51, 275, 290, 1217, 1677, 2072, 2148
HgaI	GACGC	2 cut(s)	226, 1682
HhaI	GCGC	6 cut(s)	237, 289, 415, 988, 990, 2177
Hin1I	GRCGYC	1 cut(s)	1674
Hin6I	GCGC	6 cut(s)	235, 287, 413, 986, 988, 2175
HinP1I	GCGC	6 cut(s)	235, 287, 413, 986, 988, 2175
HincII	GTYRAC	3 cut(s)	31, 136, 1093
HindII	GTYRAC	3 cut(s)	31, 136, 1093
HindIII	AAGCTT	2 cut(s)	969, 2017
HinfI	GANTC	6 cut(s)	709, 828, 1123, 1291, 1810, 2151
HpaII	CCGG	7 cut(s)	51, 275, 290, 1217, 1677, 2072, 2148
HphI	GGTGA	1 cut(s)	232
Hpy188I	TCNGA	6 cut(s)	765, 841, 1029, 1347, 1637, 2056
Hpy188III	TCNNGA	8 cut(s)	164, 257, 949, 1288, 1807, 1817, 1819, 1875
Hpy99I	CGWCG	1 cut(s)	932
HpyAV	CCTTC	5 cut(s)	310, 1341, 1492, 1498, 2087
HpyCH4III	ACNGT	3 cut(s)	125, 1321, 2167
HpyCH4IV	ACGT	4 cut(s)	613, 1146, 1662, 1728
HpySE526I	ACGT	4 cut(s)	613, 1146, 1662, 1728
Hsp92I	GRCGYC	1 cut(s)	1674
HspAI	GCGC	6 cut(s)	235, 287, 413, 986, 988, 2175
KflI	GGGWCCC	1 cut(s)	1369
KpnI	GGTACC	1 cut(s)	14
KroI	GCCGGC	3 cut(s)	289, 1676, 2071
KroNI	GCCGGC	3 cut(s)	291, 1678, 2073
Ksp22I	TGATCA	2 cut(s)	1051, 1962
KspI	CCGCGG	1 cut(s)	1951
LmnI	GCTCC	9 cut(s)	47, 278, 307, 503, 842, 1473, 1710, 1979, 2116
Lsp1109I	GCAGC	7 cut(s)	305, 739, 1639, 1911, 1966, 2204, 2207
LweI	GCATC	6 cut(s)	415, 634, 821, 999, 1543, 1896
MaeI	CTAG	6 cut(s)	269, 452, 1494, 1668, 1970, 2090
MaeII	ACGT	4 cut(s)	613, 1146, 1662, 1728
MaeIII	GTNAC	5 cut(s)	86, 196, 238, 679, 1670
MbiI	CCGCTC	1 cut(s)	575
MfeI	CAATTG	2 cut(s)	981, 1170
MflI	RGATCY	5 cut(s)	603, 765, 921, 1417, 1632
MhlI	GDGCHC	5 cut(s)	407, 734, 1440, 2007, 2103
MlyI	GAGTC	1 cut(s)	1300
MmeI	TCCRAC	3 cut(s)	559, 1201, 1668
Mph1103I	ATGCAT	1 cut(s)	649
MroNI	GCCGGC	3 cut(s)	289, 1676, 2071
MseI	TTAA	5 cut(s)	75, 398, 588, 1571, 2031
MslI	CAYNNNNRTG	3 cut(s)	1100, 1158, 1443
MspA1I	CMGCKG	5 cut(s)	752, 776, 1950, 1979, 2217
MspCI	CTTAAG	1 cut(s)	587
MspI	CCGG	7 cut(s)	51, 275, 290, 1217, 1677, 2072, 2148
MspR9I	CCNGG	4 cut(s)	8, 275, 276, 1531
MunI	CAATTG	2 cut(s)	981, 1170
Mva1269I	GAATGC	3 cut(s)	264, 1450, 1680
MvaI	CCWGG	2 cut(s)	8, 1531
MvnI	CGCG	6 cut(s)	235, 237, 305, 395, 988, 1950
NaeI	GCCGGC	3 cut(s)	291, 1678, 2073
NciI	CCSGG	2 cut(s)	275, 276
NgoMIV	GCCGGC	3 cut(s)	289, 1676, 2071
NmuCI	GTSAC	5 cut(s)	86, 196, 238, 679, 1670
NsbI	TGCGCA	1 cut(s)	414
NsiI	ATGCAT	1 cut(s)	649
NspI	RCATGY	3 cut(s)	1309, 1828, 2011
PaeI	GCATGC	1 cut(s)	1309
PaeR7I	CTCGAG	2 cut(s)	1817, 1939
PagI	TCATGA	2 cut(s)	163, 256
PauI	GCGCGC	1 cut(s)	986
PciI	ACATGT	1 cut(s)	2007
PcsI	WCGNNNNNNNCGW	1 cut(s)	1152
PctI	GAATGC	3 cut(s)	264, 1450, 1680
PdiI	GCCGGC	3 cut(s)	291, 1678, 2073
PfeI	GAWTC	5 cut(s)	709, 828, 1123, 1810, 2151
PflFI	GACNNNGTC	1 cut(s)	96
PkrI	GCNGC	9 cut(s)	295, 754, 811, 1654, 1926, 1981, 2186, 2219, 2222
PleI	GAGTC	1 cut(s)	1299
PpsI	GAGTC	1 cut(s)	1299
PpuMI	RGGWCCY	1 cut(s)	1369
PscI	ACATGT	1 cut(s)	2007
PshAI	GACNNNNGTC	1 cut(s)	1268
Psp1406I	AACGTT	1 cut(s)	1146
Psp5II	RGGWCCY	1 cut(s)	1369
Psp6I	CCWGG	2 cut(s)	6, 1529
PspGI	CCWGG	2 cut(s)	6, 1529
PspPI	GGNCC	5 cut(s)	4, 666, 883, 1369, 2074
PspPPI	RGGWCCY	1 cut(s)	1369
PspXI	VCTCGAGB	1 cut(s)	1939
PsrI	GAACNNNNNNTAC	2 cut(s)	1307, 1339
PsuI	RGATCY	5 cut(s)	603, 765, 921, 1417, 1632
PsyI	GACNNNGTC	1 cut(s)	96
PteI	GCGCGC	1 cut(s)	986
PvuII	CAGCTG	2 cut(s)	752, 776
RsaI	GTAC	8 cut(s)	12, 340, 851, 1187, 1277, 1316, 1361, 1598
RsaNI	GTAC	8 cut(s)	11, 339, 850, 1186, 1276, 1315, 1360, 1597
RseI	CAYNNNNRTG	3 cut(s)	1100, 1158, 1443
SacII	CCGCGG	1 cut(s)	1951
SaqAI	TTAA	5 cut(s)	75, 398, 588, 1571, 2031
SatI	GCNGC	9 cut(s)	294, 753, 810, 1653, 1925, 1980, 2185, 2218, 2221
Sau96I	GGNCC	5 cut(s)	4, 666, 883, 1369, 2074
SchI	GAGTC	1 cut(s)	1300
ScrFI	CCNGG	4 cut(s)	8, 275, 276, 1531
SduI	GDGCHC	5 cut(s)	407, 734, 1440, 2007, 2103
SfaNI	GCATC	6 cut(s)	415, 634, 821, 999, 1543, 1896
SfcI	CTRYAG	2 cut(s)	747, 1335
Sfr274I	CTCGAG	2 cut(s)	1817, 1939
Sfr303I	CCGCGG	1 cut(s)	1951
SgrBI	CCGCGG	1 cut(s)	1951
SinI	GGWCC	1 cut(s)	1369
SlaI	CTCGAG	2 cut(s)	1817, 1939
SmaI	CCCGGG	1 cut(s)	276
SmiMI	CAYNNNNRTG	3 cut(s)	1100, 1158, 1443
SmlI	CTYRAG	5 cut(s)	587, 1422, 1817, 1939, 2020
SmoI	CTYRAG	5 cut(s)	587, 1422, 1817, 1939, 2020
SphI	GCATGC	1 cut(s)	1309
SspMI	CTAG	6 cut(s)	269, 452, 1494, 1668, 1970, 2090
StyD4I	CCNGG	4 cut(s)	6, 273, 274, 1529
StyI	CCWWGG	3 cut(s)	498, 1969, 1989
TaaI	ACNGT	3 cut(s)	125, 1321, 2167
TaiI	ACGT	4 cut(s)	616, 1149, 1665, 1731
TatI	WGTACW	3 cut(s)	849, 1185, 1596
TauI	GCSGC	2 cut(s)	812, 2187
TfiI	GAWTC	5 cut(s)	709, 828, 1123, 1810, 2151
Tru1I	TTAA	5 cut(s)	75, 398, 588, 1571, 2031
Tru9I	TTAA	5 cut(s)	75, 398, 588, 1571, 2031
TscAI	CASTG	5 cut(s)	130, 763, 885, 1965, 2126
TseFI	GTSAC	5 cut(s)	86, 196, 238, 679, 1670
TseI	GCWGC	7 cut(s)	293, 752, 1652, 1924, 1979, 2217, 2220
Tsp45I	GTSAC	5 cut(s)	86, 196, 238, 679, 1670
TspDTI	ATGAA	6 cut(s)	245, 495, 1120, 1272, 1748, 2097
TspGWI	ACGGA	4 cut(s)	105, 945, 1268, 1601
TspMI	CCCGGG	1 cut(s)	274
TspRI	CASTG	5 cut(s)	130, 763, 885, 1965, 2126
Tth111I	GACNNNGTC	1 cut(s)	96
Vha464I	CTTAAG	1 cut(s)	587
VneI	GTGCAC	3 cut(s)	403, 1436, 2099
VpaK11BI	GGWCC	1 cut(s)	1369
XapI	RAATTY	2 cut(s)	204, 1384
XceI	RCATGY	3 cut(s)	1309, 1828, 2011
XhoI	CTCGAG	2 cut(s)	1817, 1939
XmaI	CCCGGG	1 cut(s)	274
XmaJI	CCTAGG	1 cut(s)	1969
XspI	CTAG	6 cut(s)	269, 452, 1494, 1668, 1970, 2090
Zsp2I	ATGCAT	1 cut(s)	649

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.