Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002043

Physical Location & Seq

Genomic Coordinates Forward (+)

7517474 .. 7521363

Gene Structure 3890 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1105000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2499 bp

ATGGCCCTTGTTCCTCAGAATTTCCCTAAGGCCCCTGCACCTGCTCAGAATTATAATAACCCCCCTCTACCGGCTGCTCAGAATTATAACAACCCCCCTGCTGCACCTGCTGCTCGGAATTATGATCAGGCCCCTGTACCAGCTGCTCAGAATTATACCCAGACCCCCATCCCAGCTGCTCAGAATTACAATCCGGCTCTTGTACCGGCTGCTCAGAATTATACTGCACCCGATGCCCCAGGTGCTCAGAATTACAATGCGCCCCGTGCACCAGCTGGTCAGTATTACAATAATCCCACCGCCGGAGGACGCAGAGGCGGAGACCACTACAACCGCCCTCTGCTCAAAACTGGGCGTCGCCAATCTTCAATAACTTCTGATGACAGTATGCTGACCAACCAAATTCTGGCTAGTGACAAGTCTCATGCCCGTCCCTATGATGTCACTCTTCTTTCTTTGAAACACATTCTTCAAACCGTCGACGTCATCCTGTCCCACGTTACCCAACCTGACATCCACGGCATTTTCACTGGAGCACCAGTGCCAGGTGCACATGCCGATGCTTTGGAGCATGATAAGGCTCTTTATGCTACCATGAGTGGCGTCCATGACAACTACGATGTTCCAACCAGCCTGTTTAATGAAATTTCCTGCGAGATGTTCTGCAAGTGGCAGTCCGGGGAAGATGCCAACAAGATAACCATGGACATATTAGACATTGTGCAACACTACGACTGGGATGAGAAGGTTGTTCTTGCTTTGGGAGCTTTTGCTGTGAAAGATGGTGAATATTGGCTTGTGGCTCAACTTTACACCGCCAACCCTCTTGCAAAAGCGGTTGGGCAGCTTAAGCAAGTGCAAGAGATACTGGAACGTGCCGGAACTAGCTTGAAGACCAAGTTTGACGCCTACAACAATCTGGTTAGGGCCGTGCTCAACGTGACCAAGTGCATTGTTCAGCTACATGACCTCCACCGCGATCCCCACTTGACAACTGAAGCAAAGTCAGCGGCTAACACTGCTCTTATCCCCACAGCTGTTTACTGGACGGTTCGGAGTATTGTAGTTGCTGGATCACAACTTTTGGGGATCACTGGCATGGGTCCTGAGTATGTGACAGAGACATGGGAACTATCATCCTTGGCCCATAAGCTCGAAAACATACACAGCCACCTCAAGGACAATCTCGACAGATTACAAGATATCATCCAGAGGAAGAAGGATGATGAAGCCTTAGCTGCAATTGCTTACATCTTGGAAACACCACACATTGATAACACCAAGCCTTTGAGGGTCTTGTTTTACAAGGACGATCTGCCTGCACTCTATGATTGCTACACCAAGAAGAGGGTTGACATTGATGTGCTGAAGAGGAAGGTTGTGATACTGTTCCTTTCGGACCTAGACGTTGTCCACGAAAACGAGTACATTATTGTGCAACAAATGTACATGGAAAAACGGCATAACCCAACTAGGCCAGAGAGCCAGTACGAGGTTGTGTGGGTTCCTATTGTGGACATGTGGACCGACGCCAAGTACCAGCAGTTTGAGGAAGTTAGAAGGAACATGGAATGGTTCACAGTGTTCCACCCTTCGGTTGTCTCTCCGACTGTCATCAGGTACATCAGGAAGCAGGATAAATGGAACTACGTCAAGAAGCCTCTGCTTGTGGTGATGGACCCTCAAGGAAAAATAGTGCACACCAATGCGGTTCACATGATGTGTGTTTTTGGAAGCGCTGCCTTCCCTTTCACTAGCAACAGAGAGAAGTTGCTCTGGGACGAGGAGACATGGAGGATGGAGCTTTTGGCAGACTCCCTCGATCAAAACCTAATTACCTGGATCACAGAAGGGAAATACATTTGCTTGTATGGTGGGGAAGACATAGATTGGATCAGAAACTTCACAAAGTCAGCAAAAAAAATGGCTCAAGAAGCTGGGATCCAGTTGGAATTGCTTTATGTGGGGAGGAGCAAGCCTAAGGAGAAAGTGGTCCGGAACATCATGAACATCATCCAAGCTGAGAAGCTAAGCCACACTCTTGAGTGGAACCTCATCTGGTTCTTCTGGTTGCGTCTCGAGAGCATGTGGCAGTCCAAGGGACAACAGATTCAGTCCGAGGCTTTTCGGTCAGGTCAATTTAGGACCGACAACATAAAGAATGATGCAGTGATGCAGGGGATCATATCGATGCTGAGCTTTGGTTCAAGTGACCGCGGGTGGGCTGTGATTGGCGCCGGTTCATCAGATATGTCTAAGGCTAATGGGGAGCACATGCATAGAAGTTTGAGGGAGTTTAACTTGTGGAATAGAAGGGTGAGTGAGATAGGGTTTGTGCCCGCACTGAATGAGTACCTGGCTGGGGTTTATAAAGAAGCTCCACATCACTGCACCAGTCTCATATTGCCTGCCACTGGACTCATGCCAGAGACTGTGGCCTGCGCTGAATGCGGCCGCCTCATGGAGCGGTTCACCATGTTCCGCTGCTGCACTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0008150 GO:0009888 GO:0010087 GO:0010088 GO:0032502 GO:0042802 GO:0042803 GO:0043621 GO:0046983 GO:0048856

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SEO_C SEO_N

Protein Analysis

833

Amino Acids

94.19

Weight (kDa)

6.31

Isoelectric Point (pI)

45.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SEO_N	PF14576	126 - 423	1.9e-72	Sieve element occlusion N-terminus
SEO_C	PF14577	588 - 831	3.8e-86	Sieve element occlusion C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002043)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g25290 FvH4_4g25290 FvH4_4g25290 FvH4_4g25310 FvH4_4g25320
malus_domestica	MD13G1105000.v1.1 MD13G1105100.v1.1 MD16G1104800.v1.1 MD16G1105000.v1.1
prunus_persica	Prupe.1G241400_v2.0.a1 Prupe.1G241500_v2.0.a1
pyrus_communis	pycom13g09110 pycom13g09120 pycom16g08950 pycom16g08960
rosa_chinensis	RchiOBHm_Chr4g0432561 RchiOBHm_Chr4g0432571 RchiOBHm_Chr4g0432611
rosa_laevigata	RLG00000006831 RLG00000006832 RLG00000006833
rosa_multiflora	Rmu_sc0003886.1_g000001 Rmu_sc0004612.1_g000014 Rmu_sc0027699.1_g000001
rosa_roxburghii	Rroxscaffold_5G00373990 Rroxscaffold_5G00374000 Rroxscaffold_5G00374030
rosa_rugosa	Rorug04G0260100 Rorug04G0260100 Rorug04G0260200
rosa_samantha	Rh4AG315100 Rh4AG315200 Rh4AG315300 Rh4BG322800 Rh4BG322900 Rh4BG323000 Rh4CG337900 Rh4CG338000 Rh4CG338100 Rh4DG318600 Rh4DG318700 Rh4DG318800
rosa_wichuraiana	Rw4G027380 Rw4G027390 Rw4G027420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	54, 87, 2370
AarI	CACCTGC	2 cut(s)	49, 115
AatII	GACGTC	1 cut(s)	486
Acc36I	ACCTGC	2 cut(s)	49, 115
AccB1I	GGYRCC	1 cut(s)	2234
AccB7I	CCANNNNNTGG	1 cut(s)	406
AccBSI	CCGCTC	1 cut(s)	2467
AccI	GTMKAC	1 cut(s)	480
AccII	CGCG	2 cut(s)	978, 2217
AccIII	TCCGGA	1 cut(s)	1995
AclWI	GGATC	8 cut(s)	974, 1081, 1097, 1850, 1901, 1936, 1949, 2189
AcoI	YGGCCR	1 cut(s)	2452
AcsI	RAATTY	3 cut(s)	19, 402, 645
AcuI	CTGAAG	2 cut(s)	1017, 1388
AcyI	GRCGYC	6 cut(s)	355, 483, 603, 906, 1530, 2235
AfaI	GTAC	8 cut(s)	138, 204, 1427, 1448, 1490, 1538, 1622, 2354
AfeI	AGCGCT	1 cut(s)	1738
AflII	CTTAAG	1 cut(s)	848
AflIII	ACRYGT	1 cut(s)	1518
AgsI	TTSAA	5 cut(s)	369, 460, 473, 892, 2208
AjnI	CCWGG	4 cut(s)	238, 544, 1838, 2355
Alw21I	GWGCWC	7 cut(s)	247, 271, 538, 553, 936, 1701, 2274
Alw26I	GTCTC	8 cut(s)	315, 426, 1115, 1606, 1781, 2081, 2402, 2423
Alw44I	GTGCAC	3 cut(s)	267, 549, 1697
AlwI	GGATC	8 cut(s)	974, 1081, 1097, 1850, 1901, 1936, 1949, 2189
AlwNI	CAGNNNCTG	1 cut(s)	2432
Ama87I	CYCGRG	1 cut(s)	2078
Aor13HI	TCCGGA	1 cut(s)	1995
Aor51HI	AGCGCT	1 cut(s)	1738
AoxI	GGCC	8 cut(s)	3, 30, 129, 927, 1143, 1475, 2436, 2452
ApaLI	GTGCAC	3 cut(s)	267, 549, 1697
ApoI	RAATTY	3 cut(s)	19, 402, 645
AspLEI	GCGC	4 cut(s)	262, 1739, 2237, 2444
AsuC2I	CCSGG	1 cut(s)	679
AsuHPI	GGTGA	4 cut(s)	797, 1684, 2329, 2464
AvaI	CYCGRG	1 cut(s)	2078
AvaII	GGWCC	6 cut(s)	1103, 1399, 1524, 1678, 1993, 2145
AxyI	CCTNAGG	2 cut(s)	27, 1980
BaeGI	GKGCMC	4 cut(s)	271, 553, 1701, 2340
BamHI	GGATCC	1 cut(s)	1941
BanI	GGYRCC	1 cut(s)	2234
BbsI	GAAGAC	2 cut(s)	899, 1887
Bbv12I	GWGCWC	7 cut(s)	247, 271, 538, 553, 936, 1701, 2274
BccI	CCATC	4 cut(s)	176, 776, 1669, 1792
BceAI	ACGGC	3 cut(s)	535, 914, 1475
BcgI	CGANNNNNNTGC	2 cut(s)	1301, 1335
BciT130I	CCWGG	4 cut(s)	240, 546, 1840, 2357
BclI	TGATCA	1 cut(s)	124
BcnI	CCSGG	1 cut(s)	679
BcoDI	GTCTC	8 cut(s)	315, 426, 1115, 1606, 1781, 2081, 2402, 2423
BfaI	CTAG	5 cut(s)	411, 885, 1403, 1473, 1755
BfoI	RGCGCY	2 cut(s)	1740, 2238
BfrI	CTTAAG	1 cut(s)	848
BfuAI	ACCTGC	2 cut(s)	49, 115
BlpI	GCTNAGC	2 cut(s)	2030, 2195
Bme1390I	CCNGG	5 cut(s)	240, 546, 679, 1840, 2357
Bme18I	GGWCC	6 cut(s)	1103, 1399, 1524, 1678, 1993, 2145
BmeT110I	CYCGRG	1 cut(s)	2078
BmiI	GGNNCC	8 cut(s)	33, 132, 1104, 1506, 1680, 1943, 2051, 2236
BmrFI	CCNGG	5 cut(s)	240, 546, 679, 1840, 2357
BmrI	ACTGGG	2 cut(s)	360, 745
BmsI	GCATC	6 cut(s)	223, 550, 676, 2155, 2163, 2181
BmuI	ACTGGG	2 cut(s)	360, 745
BpiI	GAAGAC	2 cut(s)	899, 1887
BpmI	CTGGAG	1 cut(s)	552
Bpu10I	CCTNAGC	1 cut(s)	1234
Bpu1102I	GCTNAGC	2 cut(s)	2030, 2195
BpuEI	CTTGAG	4 cut(s)	1160, 1668, 1914, 2063
BpuMI	CCSGG	1 cut(s)	679
Bsa29I	ATCGAT	1 cut(s)	2189
BsaHI	GRCGYC	6 cut(s)	355, 483, 603, 906, 1530, 2235
BsaI	GGTCTC	1 cut(s)	315
BsaJI	CCNNGG	8 cut(s)	238, 517, 678, 702, 1140, 2097, 2118, 2215
BsaWI	WCCGGW	1 cut(s)	1995
BsaXI	ACNNNNNCTCC	4 cut(s)	525, 555, 954, 984
Bse118I	RCCGGY	3 cut(s)	70, 205, 2237
Bse21I	CCTNAGG	2 cut(s)	27, 1980
BseAI	TCCGGA	1 cut(s)	1995
BseBI	CCWGG	4 cut(s)	240, 546, 1840, 2357
BseCI	ATCGAT	1 cut(s)	2189
BseDI	CCNNGG	8 cut(s)	238, 517, 678, 702, 1140, 2097, 2118, 2215
BseGI	GGATG	9 cut(s)	168, 486, 513, 745, 1136, 1206, 1228, 1803, 2013
BseRI	GAGGAG	2 cut(s)	1799, 1984
BseSI	GKGCMC	4 cut(s)	271, 553, 1701, 2340
BseX3I	CGGCCG	1 cut(s)	2452
BseYI	CCCAGC	3 cut(s)	172, 1937, 2360
BsgI	GTGCAG	6 cut(s)	21, 87, 210, 1305, 2374, 2473
Bsh1236I	CGCG	2 cut(s)	978, 2217
Bsh1285I	CGRYCG	1 cut(s)	2455
BshFI	GGCC	8 cut(s)	5, 32, 131, 929, 1145, 1477, 2438, 2454
BshNI	GGYRCC	1 cut(s)	2234
BshVI	ATCGAT	1 cut(s)	2189
BsiEI	CGRYCG	1 cut(s)	2455
BsiHKAI	GWGCWC	7 cut(s)	247, 271, 538, 553, 936, 1701, 2274
BsiHKCI	CYCGRG	1 cut(s)	2078
BsiSI	CCGG	8 cut(s)	71, 194, 206, 303, 678, 879, 1996, 2238
BslFI	GGGAC	4 cut(s)	417, 478, 1793, 2115
BsmAI	GTCTC	8 cut(s)	315, 426, 1115, 1606, 1781, 2081, 2402, 2423
BsmBI	CGTCTC	1 cut(s)	2081
BsmFI	GGGAC	4 cut(s)	417, 478, 1793, 2115
BsmI	GAATGC	1 cut(s)	2453
BsnI	GGCC	8 cut(s)	5, 32, 131, 929, 1145, 1477, 2438, 2454
Bso31I	GGTCTC	1 cut(s)	315
BsoBI	CYCGRG	1 cut(s)	2078
Bsp1286I	GDGCHC	8 cut(s)	247, 271, 538, 553, 936, 1701, 2274, 2340
Bsp13I	TCCGGA	1 cut(s)	1995
Bsp1407I	TGTACA	1 cut(s)	1446
Bsp1720I	GCTNAGC	2 cut(s)	2030, 2195
Bsp19I	CCATGG	1 cut(s)	702
BspANI	GGCC	8 cut(s)	5, 32, 131, 929, 1145, 1477, 2438, 2454
BspDI	ATCGAT	1 cut(s)	2189
BspEI	TCCGGA	1 cut(s)	1995
BspFNI	CGCG	2 cut(s)	978, 2217
BspHI	TCATGA	1 cut(s)	2004
BspLI	GGNNCC	8 cut(s)	33, 132, 1104, 1506, 1680, 1943, 2051, 2236
BspMI	ACCTGC	2 cut(s)	49, 115
BspPI	GGATC	8 cut(s)	974, 1081, 1097, 1850, 1901, 1936, 1949, 2189
BspT107I	GGYRCC	1 cut(s)	2234
BspTI	CTTAAG	1 cut(s)	848
BspTNI	GGTCTC	1 cut(s)	315
BsrBI	CCGCTC	1 cut(s)	2467
BsrFI	RCCGGY	3 cut(s)	70, 205, 2237
BsrGI	TGTACA	1 cut(s)	1446
BssAI	RCCGGY	3 cut(s)	70, 205, 2237
BssECI	CCNNGG	8 cut(s)	238, 517, 678, 702, 1140, 2097, 2118, 2215
BssNI	GRCGYC	6 cut(s)	355, 483, 603, 906, 1530, 2235
BssT1I	CCWWGG	3 cut(s)	702, 1140, 2097
Bst2UI	CCWGG	4 cut(s)	240, 546, 1840, 2357
Bst4CI	ACNGT	7 cut(s)	386, 478, 1051, 1389, 1582, 1612, 2434
Bst6I	CTCTTC	3 cut(s)	453, 1340, 1364
BstACI	GRCGYC	6 cut(s)	355, 483, 603, 906, 1530, 2235
BstAFI	CTTAAG	1 cut(s)	848
BstAPI	GCANNNNNTGC	2 cut(s)	110, 233
BstAUI	TGTACA	1 cut(s)	1446
BstC8I	GCNNGC	5 cut(s)	1320, 1976, 2340, 2409, 2440
BstDSI	CCRYGG	3 cut(s)	517, 702, 2215
BstF5I	GGATG	9 cut(s)	168, 486, 513, 745, 1136, 1206, 1228, 1803, 2013
BstFNI	CGCG	2 cut(s)	978, 2217
BstH2I	RGCGCY	2 cut(s)	1740, 2238
BstHHI	GCGC	4 cut(s)	262, 1739, 2237, 2444
BstMAI	GTCTC	8 cut(s)	315, 426, 1115, 1606, 1781, 2081, 2402, 2423
BstMCI	CGRYCG	1 cut(s)	2455
BstNI	CCWGG	4 cut(s)	240, 546, 1840, 2357
BstNSI	RCATGY	4 cut(s)	557, 1522, 2089, 2278
BstSCI	CCNGG	5 cut(s)	238, 544, 677, 1838, 2355
BstSLI	GKGCMC	4 cut(s)	271, 553, 1701, 2340
BstUI	CGCG	2 cut(s)	978, 2217
BstV2I	GAAGAC	2 cut(s)	899, 1887
BstX2I	RGATCY	1 cut(s)	1941
BstYI	RGATCY	1 cut(s)	1941
BstZI	CGGCCG	1 cut(s)	2452
Bsu15I	ATCGAT	1 cut(s)	2189
Bsu36I	CCTNAGG	2 cut(s)	27, 1980
BsuRI	GGCC	8 cut(s)	5, 32, 131, 929, 1145, 1477, 2438, 2454
BsuTUI	ATCGAT	1 cut(s)	2189
BtgI	CCRYGG	3 cut(s)	517, 702, 2215
BtsCI	GGATG	9 cut(s)	168, 486, 513, 745, 1136, 1206, 1228, 1803, 2013
BtsI	GCAGTG	3 cut(s)	1017, 2175, 2386
BveI	ACCTGC	2 cut(s)	49, 115
Cac8I	GCNNGC	5 cut(s)	1320, 1976, 2340, 2409, 2440
CaiI	CAGNNNCTG	1 cut(s)	2432
CciI	TCATGA	1 cut(s)	2004
CciNI	GCGGCCGC	1 cut(s)	2452
CfoI	GCGC	4 cut(s)	262, 1739, 2237, 2444
Cfr10I	RCCGGY	3 cut(s)	70, 205, 2237
Cfr42I	CCGCGG	1 cut(s)	2218
ClaI	ATCGAT	1 cut(s)	2189
CseI	GACGC	6 cut(s)	318, 344, 592, 914, 1538, 2063
Csp6I	GTAC	8 cut(s)	137, 203, 1426, 1447, 1489, 1537, 1621, 2353
CviQI	GTAC	8 cut(s)	137, 203, 1426, 1447, 1489, 1537, 1621, 2353
DinI	GGCGCC	1 cut(s)	2236
EaeI	YGGCCR	1 cut(s)	2452
EagI	CGGCCG	1 cut(s)	2452
Eam1104I	CTCTTC	3 cut(s)	453, 1340, 1364
EarI	CTCTTC	3 cut(s)	453, 1340, 1364
EciI	GGCGGA	1 cut(s)	333
EclXI	CGGCCG	1 cut(s)	2452
Eco130I	CCWWGG	3 cut(s)	702, 1140, 2097
Eco31I	GGTCTC	1 cut(s)	315
Eco32I	GATATC	1 cut(s)	1204
Eco47I	GGWCC	6 cut(s)	1103, 1399, 1524, 1678, 1993, 2145
Eco47III	AGCGCT	1 cut(s)	1738
Eco52I	CGGCCG	1 cut(s)	2452
Eco57I	CTGAAG	2 cut(s)	1017, 1388
Eco81I	CCTNAGG	2 cut(s)	27, 1980
Eco88I	CYCGRG	1 cut(s)	2078
EcoO109I	RGGNCCY	3 cut(s)	31, 130, 1103
EcoRII	CCWGG	4 cut(s)	238, 544, 1838, 2355
EcoRV	GATATC	1 cut(s)	1204
EcoT14I	CCWWGG	3 cut(s)	702, 1140, 2097
EcoT22I	ATGCAT	1 cut(s)	2280
EgeI	GGCGCC	1 cut(s)	2236
EheI	GGCGCC	1 cut(s)	2236
ErhI	CCWWGG	3 cut(s)	702, 1140, 2097
Esp3I	CGTCTC	1 cut(s)	2081
FaqI	GGGAC	4 cut(s)	417, 478, 1793, 2115
FauI	CCCGC	2 cut(s)	2210, 2347
FbaI	TGATCA	1 cut(s)	124
FblI	GTMKAC	1 cut(s)	480
FokI	GGATG	9 cut(s)	155, 473, 500, 752, 1123, 1193, 1235, 1810, 2000
FspBI	CTAG	5 cut(s)	411, 885, 1403, 1473, 1755
GlaI	GCGC	4 cut(s)	261, 1738, 2236, 2443
GsaI	CCCAGC	3 cut(s)	176, 1941, 2364
GsuI	CTGGAG	1 cut(s)	552
HaeII	RGCGCY	2 cut(s)	1740, 2238
HaeIII	GGCC	8 cut(s)	5, 32, 131, 929, 1145, 1477, 2438, 2454
HapII	CCGG	8 cut(s)	71, 194, 206, 303, 678, 879, 1996, 2238
HgaI	GACGC	6 cut(s)	318, 344, 592, 914, 1538, 2063
HhaI	GCGC	4 cut(s)	262, 1739, 2237, 2444
Hin1I	GRCGYC	6 cut(s)	355, 483, 603, 906, 1530, 2235
Hin6I	GCGC	4 cut(s)	260, 1737, 2235, 2442
HinP1I	GCGC	4 cut(s)	260, 1737, 2235, 2442
HincII	GTYRAC	2 cut(s)	481, 1354
HindII	GTYRAC	2 cut(s)	481, 1354
HinfI	GANTC	3 cut(s)	1814, 2110, 2418
HpaII	CCGG	8 cut(s)	71, 194, 206, 303, 678, 879, 1996, 2238
HphI	GGTGA	4 cut(s)	797, 1684, 2329, 2464
Hpy99I	CGWCG	4 cut(s)	360, 482, 485, 1532
HpyAV	CCTTC	8 cut(s)	739, 1213, 1369, 1554, 1602, 1753, 1844, 2307
HpyCH4III	ACNGT	7 cut(s)	386, 478, 1051, 1389, 1582, 1612, 2434
HpyCH4IV	ACGT	6 cut(s)	483, 498, 874, 939, 1407, 1650
HpySE526I	ACGT	6 cut(s)	483, 498, 874, 939, 1407, 1650
Hsp92I	GRCGYC	6 cut(s)	355, 483, 603, 906, 1530, 2235
HspAI	GCGC	4 cut(s)	260, 1737, 2235, 2442
KasI	GGCGCC	1 cut(s)	2234
Kpn2I	TCCGGA	1 cut(s)	1995
Ksp22I	TGATCA	1 cut(s)	124
KspI	CCGCGG	1 cut(s)	2218
LmnI	GCTCC	8 cut(s)	533, 568, 764, 1801, 1971, 2269, 2383, 2464
LweI	GCATC	6 cut(s)	223, 550, 676, 2155, 2163, 2181
MaeI	CTAG	5 cut(s)	411, 885, 1403, 1473, 1755
MaeII	ACGT	6 cut(s)	483, 498, 874, 939, 1407, 1650
MaeIII	GTNAC	6 cut(s)	413, 442, 499, 940, 1114, 2210
MbiI	CCGCTC	1 cut(s)	2467
MfeI	CAATTG	1 cut(s)	1242
MflI	RGATCY	1 cut(s)	1941
MhlI	GDGCHC	8 cut(s)	247, 271, 538, 553, 936, 1701, 2274, 2340
Mly113I	GGCGCC	1 cut(s)	2235
MlyI	GAGTC	2 cut(s)	1808, 2412
MmeI	TCCRAC	3 cut(s)	650, 1631, 1929
Mph1103I	ATGCAT	1 cut(s)	2280
MroI	TCCGGA	1 cut(s)	1995
MseI	TTAA	3 cut(s)	639, 849, 2298
MslI	CAYNNNNRTG	6 cut(s)	558, 1097, 1361, 1433, 1704, 2189
MspA1I	CMGCKG	7 cut(s)	143, 176, 275, 1010, 1037, 2217, 2484
MspCI	CTTAAG	1 cut(s)	848
MspI	CCGG	8 cut(s)	71, 194, 206, 303, 678, 879, 1996, 2238
MspR9I	CCNGG	5 cut(s)	240, 546, 679, 1840, 2357
MunI	CAATTG	1 cut(s)	1242
Mva1269I	GAATGC	1 cut(s)	2453
MvaI	CCWGG	4 cut(s)	240, 546, 1840, 2357
MvnI	CGCG	2 cut(s)	978, 2217
NarI	GGCGCC	1 cut(s)	2235
NciI	CCSGG	1 cut(s)	679
NcoI	CCATGG	1 cut(s)	702
NlaIV	GGNNCC	8 cut(s)	33, 132, 1104, 1506, 1680, 1943, 2051, 2236
NmuCI	GTSAC	5 cut(s)	413, 442, 940, 1114, 2210
NotI	GCGGCCGC	1 cut(s)	2452
NsiI	ATGCAT	1 cut(s)	2280
NspI	RCATGY	4 cut(s)	557, 1522, 2089, 2278
PaeR7I	CTCGAG	1 cut(s)	2078
PagI	TCATGA	1 cut(s)	2004
PaqCI	CACCTGC	2 cut(s)	49, 115
PciI	ACATGT	1 cut(s)	1518
PcsI	WCGNNNNNNNCGW	1 cut(s)	1413
PctI	GAATGC	1 cut(s)	2453
PfeI	GAWTC	1 cut(s)	2110
PflFI	GACNNNGTC	1 cut(s)	1409
PflMI	CCANNNNNTGG	1 cut(s)	406
PleI	GAGTC	2 cut(s)	1808, 2412
PluTI	GGCGCC	1 cut(s)	2238
PpsI	GAGTC	2 cut(s)	1808, 2412
PpuMI	RGGWCCY	1 cut(s)	1103
PscI	ACATGT	1 cut(s)	1518
PsiI	TTATAA	3 cut(s)	54, 87, 2370
Psp5II	RGGWCCY	1 cut(s)	1103
Psp6I	CCWGG	4 cut(s)	238, 544, 1838, 2355
PspFI	CCCAGC	3 cut(s)	172, 1937, 2360
PspGI	CCWGG	4 cut(s)	238, 544, 1838, 2355
PspN4I	GGNNCC	8 cut(s)	33, 132, 1104, 1506, 1680, 1943, 2051, 2236
PspPPI	RGGWCCY	1 cut(s)	1103
PstNI	CAGNNNCTG	1 cut(s)	2432
PsuI	RGATCY	1 cut(s)	1941
PsyI	GACNNNGTC	1 cut(s)	1409
PvuII	CAGCTG	4 cut(s)	143, 176, 275, 1037
RsaI	GTAC	8 cut(s)	138, 204, 1427, 1448, 1490, 1538, 1622, 2354
RsaNI	GTAC	8 cut(s)	137, 203, 1426, 1447, 1489, 1537, 1621, 2353
RseI	CAYNNNNRTG	6 cut(s)	558, 1097, 1361, 1433, 1704, 2189
SacII	CCGCGG	1 cut(s)	2218
SalI	GTCGAC	1 cut(s)	479
SaqAI	TTAA	3 cut(s)	639, 849, 2298
SchI	GAGTC	2 cut(s)	1808, 2412
ScrFI	CCNGG	5 cut(s)	240, 546, 679, 1840, 2357
SduI	GDGCHC	8 cut(s)	247, 271, 538, 553, 936, 1701, 2274, 2340
SfaNI	GCATC	6 cut(s)	223, 550, 676, 2155, 2163, 2181
SfoI	GGCGCC	1 cut(s)	2236
Sfr274I	CTCGAG	1 cut(s)	2078
Sfr303I	CCGCGG	1 cut(s)	2218
SgrBI	CCGCGG	1 cut(s)	2218
SgrDI	CGTCGACG	1 cut(s)	479
SinI	GGWCC	6 cut(s)	1103, 1399, 1524, 1678, 1993, 2145
SlaI	CTCGAG	1 cut(s)	2078
SmiMI	CAYNNNNRTG	6 cut(s)	558, 1097, 1361, 1433, 1704, 2189
SmlI	CTYRAG	6 cut(s)	848, 1175, 1683, 1929, 2042, 2078
SmoI	CTYRAG	6 cut(s)	848, 1175, 1683, 1929, 2042, 2078
SspDI	GGCGCC	1 cut(s)	2234
SspI	AATATT	1 cut(s)	791
SspMI	CTAG	5 cut(s)	411, 885, 1403, 1473, 1755
StyD4I	CCNGG	5 cut(s)	238, 544, 677, 1838, 2355
StyI	CCWWGG	3 cut(s)	702, 1140, 2097
TaaI	ACNGT	7 cut(s)	386, 478, 1051, 1389, 1582, 1612, 2434
TaiI	ACGT	6 cut(s)	486, 501, 877, 942, 1410, 1653
TaqI	TCGA	6 cut(s)	480, 1155, 1188, 1821, 2079, 2189
TaqII	GACCGA	3 cut(s)	1541, 2118, 2162
TatI	WGTACW	2 cut(s)	1425, 1446
TauI	GCSGC	3 cut(s)	1013, 2454, 2457
TfiI	GAWTC	1 cut(s)	2110
Tru1I	TTAA	3 cut(s)	639, 849, 2298
Tru9I	TTAA	3 cut(s)	639, 849, 2298
TseFI	GTSAC	5 cut(s)	413, 442, 940, 1114, 2210
Tsp45I	GTSAC	5 cut(s)	413, 442, 940, 1114, 2210
TspDTI	ATGAA	4 cut(s)	657, 1242, 2021, 2232
Tth111I	GACNNNGTC	1 cut(s)	1409
Van91I	CCANNNNNTGG	1 cut(s)	406
Vha464I	CTTAAG	1 cut(s)	848
VneI	GTGCAC	3 cut(s)	267, 549, 1697
VpaK11BI	GGWCC	6 cut(s)	1103, 1399, 1524, 1678, 1993, 2145
XapI	RAATTY	3 cut(s)	19, 402, 645
XceI	RCATGY	4 cut(s)	557, 1522, 2089, 2278
XcmI	CCANNNNNNNNNTGG	1 cut(s)	403
XhoI	CTCGAG	1 cut(s)	2078
XmiI	GTMKAC	1 cut(s)	480
XspI	CTAG	5 cut(s)	411, 885, 1403, 1473, 1755
ZraI	GACGTC	1 cut(s)	484
Zsp2I	ATGCAT	1 cut(s)	2280

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.