FvH4_4g29860

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002639
Physical Location & Seq
Reverse (-)
29792355 .. 29797305
4951 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g29860.t1

Sequence Viewer

Length: 2145 bp
ATGGCTCACGGACGGCTCAACAACTCCGCCAACGGCCAAAAACGCAGCTTCTCCGACGAGAATCGTAAACCCGACGACTTCGCCAAGAAGCAGAAGCTTACGCAGCACAGGAAGTCTCTGCCGATCGCTTCTGTTGAGAAGCGTCTCGTAGAGGAGGTACGAAAGCATGACACTTTGATTATTGTTGGTGAAACCGGAAGCGGAAAAACAACGCAATTACCGCAGTTTTTGTACAATGCTGGATTTTGTGGGTATGGGAAAGTCATTGGGGTGACTCAACCTAGGCGTGTGGCTGCTATGACTGTGGCAAAGCGGGTGGCTGAGGAATGCGGTGTTGCTTTGGGTGAAAAGGTTGGTTATTCTGTGAGATTTGATGATGTGACCTCTAGTTCAACGAGGATCAAGTATATGACAGATGGATTGCTACTGAGGGAAGCATTATTGGATCCATATCTATCTAAGTATTCAGTTGTCATTGTCGATGAAGCTCACGAGAGAACTGTCCAGACTGATGTCTTACTCGGCTTGCTTAAAAATGTACAAAATGCAAGGGCAAAAGTTGTGAATAACCACCTAGATACAAAAAACAAGAAAACAAGTAATGACATGCAGTTGGATAAAGAAAATGGTTCCCAGACTTTTAGTTTCTTGAAGCAATGCCAAGGAAAGAATCCATTGAAGTTAATTATCATGTCTGCTAGTTTGGATGCACGAATTTTCTCCGAGTACTTTGGTGGTGCAAGAGCTGTTCACATCCAAGGTCGACAATTTCCTGTCGATATATATTATACTCATCATAATGTACAAGATTATCTAGATGCAGCGTTAACTGCCATATTTCAGATTCATATGGAGGAGGACCCTGGTGATGTACTTGTATTTTTGACTGGTCAAGAAGAAATTGAATCTGTACAGAGACTTGTCAAAGAGAGACTTAAACAGTTACCTGAAACAAGTCAAAAACTAGTAGCTGTTCCTATATTCTCATCTCTTCCATCAGAACAGCAAATGCAAGTGTTTGCACCAGCTCCTGATGGATTTCGTAAGGTAATATTGGCAACGAATATCGCTGAGACATCTGTCACAATACCTGGAATCAAGTATGTTATAGATCCTGGGTTCGTAAAAGCACGTACCTATGATCCGAATAAAGGGTTATCAAGTTTGAATGTTATTCCAATTTCAAAAGCGCAGGCTCTTCAGAGGAGTGGGCGTGCAGGGAGAGAGAGATCAGGGAAGTGCTTCCGCCTCTACCCAGAAAATCAATTTGAGAAACTTGAGGATTCAACAAAGCCAGAGATCAAGCGGTGCGACCTCGCTAGTATAATTCTGCAACTTAAGGTTCTGGGCGTTGATGATGTCATTGGGTTCGACTTCATAGATAAACCTTCAAGGTCGGCTATTGTCAAATCACTGGAGCAATTGTATGTGCTAGGTGCTTTAACAGATGAGTGCAAACTGTCAGAAGTTGGACGTCAAATGGCAAGCCTTCCGCTAGACCCTATTTACTCAAAAGCTCTAATCCGAGCAAGTGAAACTGAATTCAATTGCCTTGAAGAGATGTTGATAACTGTTGCAATGCTCTCAGTCGAGTCTATATTTTTCGCTCCCCGTGAGAAATTAAACGAGGCCAGAATTGCCATGAAGTGCTTTGCAAGTCCAGATGGGGACCACCTCACTTTGGTTAATGTATATCGAGCGTTTAGTGAGTTCTCAGAGAAGAGATCAGATCTCAGTAAAGAGAAACATGAAAAAAGTATCAGGAAATGGTGCAAGGACAATTTCATTAATAGTCGCTCCCTGAAGCATGCTCGTGACATACATAGCCAAATCCGAGGAAATGTTGAACAAATGGGTCTTCATGTAGCTTCCTGCGGGGATGACATGCTTCCGTTTCGAAGATGTCTTGCTGCCTCTTTCTTCCTCAATGCAGCATTAAAACAGCCTGAGGGAACATACAGGGCTTTAGCAAATGGTCAGGTGGTTCGGATCCATCCTTCGTCTGTGTTGTTCCATGAGAAACCAGAGTGTGTAGTATTTAATGAATTAGTCGAAACTAATCATAAATACATCCGCAACACAACTAGAATCGATTATTTGTGGTTACATGAGCTTGCTCCTCATTTCTATGCCATGCAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0001817 GO:0001819 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003723 GO:0003724 GO:0003725 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006356 GO:0006396 GO:0006412 GO:0006413 GO:0006518 GO:0006725 GO:0006807 GO:0008026 GO:0008134 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0023056 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032479 GO:0032481 GO:0032991 GO:0033613 GO:0034641 GO:0034645 GO:0042325 GO:0042327 GO:0042623 GO:0043021 GO:0043023 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0043408 GO:0043410 GO:0043484 GO:0043565 GO:0043603 GO:0043604 GO:0044087 GO:0044089 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045937 GO:0045943 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051090 GO:0051091 GO:0051092 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0060255 GO:0061702 GO:0065007 GO:0065009 GO:0070013 GO:0070035 GO:0071704 GO:0072559 GO:0080090 GO:0090304 GO:0097159 GO:0140098 GO:1900225 GO:1900227 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03040 ko03050 ko05169 map03040 map03050 map05169
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

715

Amino Acids

80.41

Weight (kDa)

8.81

Isoelectric Point (pI)

37.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 278 - 408 2.3e-12 Helicase conserved C-terminal domain
WHD_HA2 PF04408 471 - 499 2.6e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 500 - 561 1.8e-11 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 633 - 708 3.9e-22 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1477
AccI GTMKAC 1 cut(s) 763
AclWI GGATC 7 cut(s) 407, 440, 453, 1106, 1136, 1984, 1997
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 2 cut(s) 714, 1541
AcuI CTGAAG 2 cut(s) 1184, 1823
AcyI GRCGYC 1 cut(s) 1474
AfaI GTAC 8 cut(s) 159, 233, 540, 728, 804, 873, 912, 1135
AfiI CCNNNNNNNGG 2 cut(s) 1151, 1681
AflII CTTAAG 1 cut(s) 1337
AhlI ACTAGT 1 cut(s) 964
AjnI CCWGG 3 cut(s) 862, 1090, 1114
AluBI AGCT 9 cut(s) 48, 97, 488, 746, 971, 1028, 1517, 1868, 2113
AluI AGCT 9 cut(s) 48, 97, 488, 746, 971, 1028, 1517, 1868, 2113
Alw26I GTCTC 5 cut(s) 120, 149, 910, 925, 1067
AlwI GGATC 7 cut(s) 407, 440, 453, 1106, 1136, 1984, 1997
AlwNI CAGNNNCTG 1 cut(s) 1031
AoxI GGCC 2 cut(s) 34, 1629
ApeKI GCWGC 6 cut(s) 45, 103, 293, 821, 1910, 1931
ApoI RAATTY 2 cut(s) 714, 1541
AseI ATTAAT 1 cut(s) 1788
Asp700I GAANNNNTTC 1 cut(s) 1241
AspA2I CCTAGG 1 cut(s) 281
AspLEI GCGC 1 cut(s) 1192
AspS9I GGNCC 2 cut(s) 859, 1669
AsuHPI GGTGA 4 cut(s) 200, 283, 356, 878
AsuII TTCGAA 1 cut(s) 1897
AvaII GGWCC 2 cut(s) 859, 1669
AvrII CCTAGG 1 cut(s) 281
AxyI CCTNAGG 1 cut(s) 1947
BamHI GGATCC 2 cut(s) 445, 1989
BauI CACGAG 2 cut(s) 491, 1812
BbsI GAAGAC 1 cut(s) 1850
BbvCI CCTCAGC 1 cut(s) 321
BbvI GCAGC 6 cut(s) 57, 115, 280, 833, 1897, 1943
BccI CCATC 5 cut(s) 410, 1003, 1028, 1658, 2001
BceAI ACGGC 2 cut(s) 29, 49
BciT130I CCWGG 3 cut(s) 864, 1092, 1116
BcoDI GTCTC 5 cut(s) 120, 149, 910, 925, 1067
BcuI ACTAGT 1 cut(s) 964
BfrI CTTAAG 1 cut(s) 1337
BglII AGATCT 1 cut(s) 1729
BisI GCNGC 6 cut(s) 46, 104, 294, 822, 1911, 1932
BlnI CCTAGG 1 cut(s) 281
BlsI GCNGC 6 cut(s) 47, 105, 295, 823, 1912, 1933
BmcAI AGTACT 1 cut(s) 728
Bme1390I CCNGG 3 cut(s) 864, 1092, 1116
Bme18I GGWCC 2 cut(s) 859, 1669
BmgT120I GGNCC 2 cut(s) 859, 1669
BmiI GGNNCC 5 cut(s) 447, 631, 861, 1670, 1991
BmrFI CCNGG 3 cut(s) 864, 1092, 1116
BmsI GCATC 2 cut(s) 697, 808
BoxI GACNNNNGTC 2 cut(s) 512, 1079
BpiI GAAGAC 1 cut(s) 1850
BpmI CTGGAG 1 cut(s) 1436
Bpu10I CCTNAGC 1 cut(s) 321
Bpu14I TTCGAA 1 cut(s) 1897
BpuEI CTTGAG 1 cut(s) 1298
Bsa29I ATCGAT 1 cut(s) 2091
BsaAI YACGTR 1 cut(s) 1133
BsaBI GATNNNNATC 1 cut(s) 450
BsaHI GRCGYC 1 cut(s) 1474
BsaJI CCNNGG 6 cut(s) 281, 661, 757, 862, 1115, 1834
BsaWI WCCGGW 1 cut(s) 194
BsaXI ACNNNNNCTCC 4 cut(s) 1198, 1228, 1409, 1439
Bsc4I CCNNNNNNNGG 2 cut(s) 1151, 1681
Bse1I ACTGG 2 cut(s) 892, 1419
Bse21I CCTNAGG 1 cut(s) 1947
Bse3DI GCAATG 2 cut(s) 662, 1584
Bse8I GATNNNNATC 1 cut(s) 450
BseBI CCWGG 3 cut(s) 864, 1092, 1116
BseCI ATCGAT 1 cut(s) 2091
BseDI CCNNGG 6 cut(s) 281, 661, 757, 862, 1115, 1834
BseGI GGATG 5 cut(s) 712, 753, 1885, 1993, 2070
BseJI GATNNNNATC 1 cut(s) 450
BseLI CCNNNNNNNGG 2 cut(s) 1151, 1681
BseMI GCAATG 2 cut(s) 662, 1584
BseMII CTCAG 7 cut(s) 312, 419, 1062, 1599, 1728, 1747, 1938
BseNI ACTGG 2 cut(s) 892, 1419
BseRI GAGGAG 4 cut(s) 167, 869, 1219, 2109
BseXI GCAGC 6 cut(s) 57, 115, 280, 833, 1897, 1943
BsgI GTGCAG 1 cut(s) 1236
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 2 cut(s) 36, 1631
BshVI ATCGAT 1 cut(s) 2091
BsiEI CGRYCG 1 cut(s) 126
BsiSI CCGG 1 cut(s) 195
BslFI GGGAC 1 cut(s) 1682
BslI CCNNNNNNNGG 2 cut(s) 1151, 1681
BsmAI GTCTC 5 cut(s) 120, 149, 910, 925, 1067
BsmBI CGTCTC 1 cut(s) 149
BsmFI GGGAC 1 cut(s) 1682
BsmI GAATGC 1 cut(s) 332
BsnI GGCC 2 cut(s) 36, 1631
Bsp119I TTCGAA 1 cut(s) 1897
Bsp1407I TGTACA 4 cut(s) 231, 538, 802, 910
BspANI GGCC 2 cut(s) 36, 1631
BspCNI CTCAG 7 cut(s) 313, 420, 1063, 1598, 1727, 1746, 1939
BspDI ATCGAT 1 cut(s) 2091
BspLI GGNNCC 5 cut(s) 447, 631, 861, 1670, 1991
BspPI GGATC 7 cut(s) 407, 440, 453, 1106, 1136, 1984, 1997
BspQI GCTCTTC 1 cut(s) 1203
BspT104I TTCGAA 1 cut(s) 1897
BspTI CTTAAG 1 cut(s) 1337
BsrDI GCAATG 2 cut(s) 662, 1584
BsrGI TGTACA 4 cut(s) 231, 538, 802, 910
BsrI ACTGG 2 cut(s) 892, 1419
BssECI CCNNGG 6 cut(s) 281, 661, 757, 862, 1115, 1834
BssNI GRCGYC 1 cut(s) 1474
BssSI CACGAG 2 cut(s) 491, 1812
BssT1I CCWWGG 3 cut(s) 281, 661, 757
Bst2BI CACGAG 2 cut(s) 491, 1812
Bst2UI CCWGG 3 cut(s) 864, 1092, 1116
Bst4CI ACNGT 5 cut(s) 304, 502, 942, 1461, 1573
Bst6I CTCTTC 4 cut(s) 996, 1203, 1551, 1715
BstACI GRCGYC 1 cut(s) 1474
BstAFI CTTAAG 1 cut(s) 1337
BstAUI TGTACA 4 cut(s) 231, 538, 802, 910
BstBAI YACGTR 1 cut(s) 1133
BstBI TTCGAA 1 cut(s) 1897
BstC8I GCNNGC 6 cut(s) 527, 1194, 1215, 1486, 1809, 2115
BstDEI CTNAG 8 cut(s) 321, 428, 459, 1071, 1585, 1714, 1733, 1947
BstF5I GGATG 5 cut(s) 712, 753, 1885, 1993, 2070
BstHHI GCGC 1 cut(s) 1192
BstMAI GTCTC 5 cut(s) 120, 149, 910, 925, 1067
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 5 cut(s) 42, 103, 220, 830, 1637
BstNI CCWGG 3 cut(s) 864, 1092, 1116
BstNSI RCATGY 3 cut(s) 610, 1811, 1888
BstPAI GACNNNNGTC 2 cut(s) 512, 1079
BstSCI CCNGG 3 cut(s) 862, 1090, 1114
BstV1I GCAGC 6 cut(s) 57, 115, 280, 833, 1897, 1943
BstV2I GAAGAC 1 cut(s) 1850
BstX2I RGATCY 4 cut(s) 445, 1111, 1729, 1989
BstYI RGATCY 4 cut(s) 445, 1111, 1729, 1989
Bsu15I ATCGAT 1 cut(s) 2091
Bsu36I CCTNAGG 1 cut(s) 1947
BsuRI GGCC 2 cut(s) 36, 1631
BsuTUI ATCGAT 1 cut(s) 2091
BtsCI GGATG 5 cut(s) 712, 753, 1885, 1993, 2070
BtsIMutI CAGTG 1 cut(s) 1412
Cac8I GCNNGC 6 cut(s) 527, 1194, 1215, 1486, 1809, 2115
CaiI CAGNNNCTG 1 cut(s) 1031
CfoI GCGC 1 cut(s) 1192
Cfr13I GGNCC 2 cut(s) 859, 1669
ClaI ATCGAT 1 cut(s) 2091
CseI GACGC 1 cut(s) 131
Csp6I GTAC 8 cut(s) 158, 232, 539, 727, 803, 872, 911, 1134
CspCI CAANNNNNGTGG 2 cut(s) 297, 332
CviQI GTAC 8 cut(s) 158, 232, 539, 727, 803, 872, 911, 1134
DdeI CTNAG 8 cut(s) 321, 428, 459, 1071, 1585, 1714, 1733, 1947
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 4 cut(s) 996, 1203, 1551, 1715
EarI CTCTTC 4 cut(s) 996, 1203, 1551, 1715
EciI GGCGGA 2 cut(s) 16, 1235
Eco130I CCWWGG 3 cut(s) 281, 661, 757
Eco47I GGWCC 2 cut(s) 859, 1669
Eco57I CTGAAG 2 cut(s) 1184, 1823
Eco81I CCTNAGG 1 cut(s) 1947
EcoO109I RGGNCCY 1 cut(s) 859
EcoRI GAATTC 1 cut(s) 1541
EcoRII CCWGG 3 cut(s) 862, 1090, 1114
EcoT14I CCWWGG 3 cut(s) 281, 661, 757
ErhI CCWWGG 3 cut(s) 281, 661, 757
Esp3I CGTCTC 1 cut(s) 149
FalI AAGNNNNNCTT 2 cut(s) 918, 950
FaqI GGGAC 1 cut(s) 1682
FauI CCCGC 2 cut(s) 306, 1868
FauNDI CATATG 1 cut(s) 849
FblI GTMKAC 1 cut(s) 763
Fnu4HI GCNGC 6 cut(s) 46, 104, 294, 822, 1911, 1932
FokI GGATG 5 cut(s) 719, 740, 1892, 1980, 2057
Fsp4HI GCNGC 6 cut(s) 46, 104, 294, 822, 1911, 1932
GlaI GCGC 1 cut(s) 1191
GluI GCNGC 6 cut(s) 46, 104, 294, 822, 1911, 1932
GsuI CTGGAG 1 cut(s) 1436
HaeIII GGCC 2 cut(s) 36, 1631
HapII CCGG 1 cut(s) 195
HgaI GACGC 1 cut(s) 131
HhaI GCGC 1 cut(s) 1192
Hin1I GRCGYC 1 cut(s) 1474
Hin6I GCGC 1 cut(s) 1190
HinP1I GCGC 1 cut(s) 1190
HincII GTYRAC 2 cut(s) 764, 828
HindII GTYRAC 2 cut(s) 764, 828
HindIII AAGCTT 1 cut(s) 95
HinfI GANTC 9 cut(s) 61, 274, 670, 844, 905, 1095, 1283, 1592, 2088
HpaI GTTAAC 1 cut(s) 828
HpaII CCGG 1 cut(s) 195
HphI GGTGA 4 cut(s) 200, 283, 356, 878
Hpy166II GTNNAC 4 cut(s) 68, 751, 764, 828
Hpy188III TCNNGA 9 cut(s) 491, 505, 649, 815, 893, 1031, 1661, 1762, 1814
Hpy8I GTNNAC 4 cut(s) 68, 751, 764, 828
Hpy99I CGWCG 2 cut(s) 59, 77
HpyAV CCTTC 3 cut(s) 1398, 1499, 2007
HpyCH4III ACNGT 5 cut(s) 304, 502, 942, 1461, 1573
HpyCH4IV ACGT 2 cut(s) 1132, 1474
HpyF10VI GCNNNNNNNGC 5 cut(s) 42, 103, 220, 830, 1637
HpyF3I CTNAG 8 cut(s) 321, 428, 459, 1071, 1585, 1714, 1733, 1947
HpySE526I ACGT 2 cut(s) 1132, 1474
Hsp92I GRCGYC 1 cut(s) 1474
HspAI GCGC 1 cut(s) 1190
KspAI GTTAAC 1 cut(s) 828
LguI GCTCTTC 1 cut(s) 1203
LmnI GCTCC 5 cut(s) 1033, 1417, 1612, 1802, 2122
Lsp1109I GCAGC 6 cut(s) 57, 115, 280, 833, 1897, 1943
LweI GCATC 2 cut(s) 697, 808
MaeII ACGT 2 cut(s) 1132, 1474
MaeIII GTNAC 6 cut(s) 271, 379, 942, 1081, 1814, 2103
MboII GAAGA 8 cut(s) 908, 983, 1190, 1568, 1732, 1850, 1911, 1912
MfeI CAATTG 2 cut(s) 1421, 1546
MflI RGATCY 4 cut(s) 445, 1111, 1729, 1989
MlyI GAGTC 2 cut(s) 268, 1601
MmeI TCCRAC 3 cut(s) 78, 594, 1450
MroXI GAANNNNTTC 1 cut(s) 1241
MslI CAYNNNNRTG 4 cut(s) 269, 798, 1812, 2127
MspCI CTTAAG 1 cut(s) 1337
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 3 cut(s) 864, 1092, 1116
MunI CAATTG 2 cut(s) 1421, 1546
Mva1269I GAATGC 1 cut(s) 332
MvaI CCWGG 3 cut(s) 864, 1092, 1116
MwoI GCNNNNNNNGC 5 cut(s) 42, 103, 220, 830, 1637
NdeI CATATG 1 cut(s) 849
NlaIV GGNNCC 5 cut(s) 447, 631, 861, 1670, 1991
NmeAIII GCCGAG 1 cut(s) 501
NmuCI GTSAC 4 cut(s) 271, 379, 1081, 1814
NspI RCATGY 3 cut(s) 610, 1811, 1888
NspV TTCGAA 1 cut(s) 1897
PaeI GCATGC 1 cut(s) 1811
PciSI GCTCTTC 1 cut(s) 1203
PctI GAATGC 1 cut(s) 332
PdmI GAANNNNTTC 1 cut(s) 1241
PfeI GAWTC 7 cut(s) 61, 670, 844, 905, 1095, 1283, 2088
PkrI GCNGC 6 cut(s) 47, 105, 295, 823, 1912, 1933
Ple19I CGATCG 1 cut(s) 126
PleI GAGTC 2 cut(s) 268, 1600
PpsI GAGTC 2 cut(s) 268, 1600
Ppu21I YACGTR 1 cut(s) 1133
PpuMI RGGWCCY 1 cut(s) 859
PshAI GACNNNNGTC 2 cut(s) 512, 1079
PshBI ATTAAT 1 cut(s) 1788
Psp5II RGGWCCY 1 cut(s) 859
Psp6I CCWGG 3 cut(s) 862, 1090, 1114
PspGI CCWGG 3 cut(s) 862, 1090, 1114
PspN4I GGNNCC 5 cut(s) 447, 631, 861, 1670, 1991
PspPI GGNCC 2 cut(s) 859, 1669
PspPPI RGGWCCY 1 cut(s) 859
PstNI CAGNNNCTG 1 cut(s) 1031
PsuI RGATCY 4 cut(s) 445, 1111, 1729, 1989
PvuI CGATCG 1 cut(s) 126
RsaI GTAC 8 cut(s) 159, 233, 540, 728, 804, 873, 912, 1135
RsaNI GTAC 8 cut(s) 158, 232, 539, 727, 803, 872, 911, 1134
RseI CAYNNNNRTG 4 cut(s) 269, 798, 1812, 2127
SalI GTCGAC 1 cut(s) 762
SapI GCTCTTC 1 cut(s) 1203
SatI GCNGC 6 cut(s) 46, 104, 294, 822, 1911, 1932
Sau96I GGNCC 2 cut(s) 859, 1669
ScaI AGTACT 1 cut(s) 728
SchI GAGTC 2 cut(s) 268, 1601
ScrFI CCNGG 3 cut(s) 864, 1092, 1116
SfaNI GCATC 2 cut(s) 697, 808
SfuI TTCGAA 1 cut(s) 1897
SinI GGWCC 2 cut(s) 859, 1669
SmiMI CAYNNNNRTG 4 cut(s) 269, 798, 1812, 2127
SmlI CTYRAG 2 cut(s) 1277, 1337
SmoI CTYRAG 2 cut(s) 1277, 1337
SpeI ACTAGT 1 cut(s) 964
SphI GCATGC 1 cut(s) 1811
SspI AATATT 1 cut(s) 1053
StyD4I CCNGG 3 cut(s) 862, 1090, 1114
StyI CCWWGG 3 cut(s) 281, 661, 757
TaaI ACNGT 5 cut(s) 304, 502, 942, 1461, 1573
TaiI ACGT 2 cut(s) 1135, 1477
TaqI TCGA 9 cut(s) 480, 763, 777, 1371, 1590, 1696, 1897, 2052, 2091
TatI WGTACW 6 cut(s) 231, 538, 726, 802, 871, 910
TfiI GAWTC 7 cut(s) 61, 670, 844, 905, 1095, 1283, 2088
TscAI CASTG 1 cut(s) 1419
TseFI GTSAC 4 cut(s) 271, 379, 1081, 1814
TseI GCWGC 6 cut(s) 45, 103, 293, 821, 1910, 1931
Tsp45I GTSAC 4 cut(s) 271, 379, 1081, 1814
TspDTI ATGAA 8 cut(s) 498, 836, 1366, 1658, 1764, 1774, 1850, 2058
TspGWI ACGGA 2 cut(s) 24, 1881
TspRI CASTG 1 cut(s) 1419
Vha464I CTTAAG 1 cut(s) 1337
VpaK11BI GGWCC 2 cut(s) 859, 1669
VspI ATTAAT 1 cut(s) 1788
XapI RAATTY 2 cut(s) 714, 1541
XbaI TCTAGA 1 cut(s) 814
XceI RCATGY 3 cut(s) 610, 1811, 1888
XmaJI CCTAGG 1 cut(s) 281
XmiI GTMKAC 1 cut(s) 763
XmnI GAANNNNTTC 1 cut(s) 1241
ZraI GACGTC 1 cut(s) 1475
ZrmI AGTACT 1 cut(s) 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.