Rh4CG383600

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0002639
Physical Location & Seq
Reverse (-)
63655489 .. 63659488
4000 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG383600.1

Sequence Viewer

Length: 2148 bp
ATGGCTCACGGACGGCTCAATAACTCCACCAGCGGCCTTAAACGCAGCTTCTCCGACGAGAATCGTAAACCCGACGCCTTTGCCAAGAGGCAGAAGCTTTCGCAGCACAGGAAGGCTCTCCCGATCGCTTCTGTTGAGAAGCGTCTGGTAGAGGAAGTTAGAAAGCATGATACTTTGATTATTGTTGGCGAAACTGGAAGCGGAAAAACAACACAATTACCTCAGTTTTTGTACAATGCTGGATTTTGTGGGTATGGGAAAGTCATTGGGGTAACTCAACCTAGGCGTGTGGCTGCTATGACCGTGGCGAAACGGGTTGCTGAGGAATGCGGTGTTACTTTGGGTGAAAAGGTTGGTTACGCTGTCAGATTTGAGGATGTGACCTCTAGTTCAACAAGGATCAAGTATATGACTGATGGATTGCTACTGAGGGAAGCACTATTGGATCCATATCTCTCTAAGTATTCAGTCGTCATTGTCGATGAAGCTCATGAGAGAACTGTCCAGACTGATGTCTTACTGGGCTTGCTTAAAAATGTACAAAATGCAAGGTCAAAAGTTGTCAATAACCACCAAGATACAAAAAACACGAAGACAAATAATGACATGCAGTTGGATAAAGAAAATGGTTCTCAGTCCTTGAGTTTTCTCAAGCAATGCCAAGGAAGGAATCCATTGAAGTTAATTATCATGTCTGCCAGTTTGGATGCACGAATTTTCTCCGAGTACTTCGGTGGTGCAAGAGCTGTTCACATCCAGGGTCGACAGTTTCCTGTCGATATATATTATACTCATCACAATGTACAAGATTATCTAGATGCATCGTTAACTGCCATATTTCAGATTCATATGGAGGAGGACCCTGGTGATATACTTGTATTTTTGACTGGTCAAGAAGAAATTGAATCTGTACAGAGACTTGTCAAAGAAAGACTTAAACAGTTACCTGAAACCAGTCAAAAACTCGTAGCTGTTCCTATATTCTCATCTCTTCCATCAGAACAGCAAATGCAAGTGTTTGCACCGGCTCCTGATGGATTTCGTAAGGTAATATTGGCAACGAATATTGCTGAGACATCTGTGACAATACCTGGAATCAAGTATGTAATAGATCCTGGGTTTGTAAAAGCACGTACCTATGATCCGAATAAAGGGTTATCAAGTTTGAATGTTATTCCAATTTCAAAAGCACAGGCTCTTCAGAGAAGTGGGCGTGCAGGGAGAGAAAGATCAGGGAAGTGCTTCCGCCTCTACCCAGAGAATCAATTTGAGAAACTTGAGGATTCAACAAAGCCAGAGATCAAGCGGTGTGACCTCTCTAGTATAATTTTGCAACTTAAGGTTCTGGGTGTTGATGATGTCATTGGGTTCGACTTCATAGATAAACCTTCAAGGTCGGCTATTGTCAAATCACTGGAGCAATTGTATGTGCTAGGTGCTTTAACAGATGAGTGTAAACTATCAGATCCAGTTGGACGTCAAATGGCAAGCCTTCCCCTAGACCCTATTTTCTCAAAAGCTCTAATCCGAGCAAGTGAAACTGAATTCAATTGCCTCGAAGAGATGTTGATAACTGTTGCAATGCTCTCAGTTGAGTCCATATTTTTCGCTCCCCGTGAGAAATTGAACGAGGCTAGAACTGCAATGAAATGCTTTGCAAGTCCAGATGGGGACCACCTCACTTTGGTTAATGTATATCGAGCGTTTAATGAGTTTTCAGGGAAGAGATCAGATCTCAGTAAAGAGAAACATGAAAAAAGTCTCAGGAAATGGTGCAAGGACAATTTCATTAATAGTCGCTCCCTGAAGCATGCTCGTGACATTCACAGCCAAATCCGAGGGAATGTTGAACAAATGGGTCTTCATGTAGCTTCTTGCGGGGATGACATGCTTCCGTTTCGAAGATGTCTTGCAGCGTCTTTCTTCCTCAATGCAGCTTTAAAACAGCCCGAGGGAACTTACAGGGCTTTAGCAAATGGTCAGGTGGTTCGGATTCATCCGTCGTCCGTGTTGTTCCATGAGAAACCAGAGTGTATAGTTTTTAATGAACTAGTCGAAACTAATCATAAATACATCCGCAACACAACTAGAATCGATTATTTATGGTTACATGAGCTTGCTCCTCATTTCTATGCCATGCAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0001817 GO:0001819 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003723 GO:0003724 GO:0003725 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006356 GO:0006396 GO:0006412 GO:0006413 GO:0006518 GO:0006725 GO:0006807 GO:0008026 GO:0008134 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0023056 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032479 GO:0032481 GO:0032991 GO:0033613 GO:0034641 GO:0034645 GO:0042325 GO:0042327 GO:0042623 GO:0043021 GO:0043023 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0043408 GO:0043410 GO:0043484 GO:0043565 GO:0043603 GO:0043604 GO:0044087 GO:0044089 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045937 GO:0045943 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051090 GO:0051091 GO:0051092 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0060255 GO:0061702 GO:0065007 GO:0065009 GO:0070013 GO:0070035 GO:0071704 GO:0072559 GO:0080090 GO:0090304 GO:0097159 GO:0140098 GO:1900225 GO:1900227 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03040 ko03050 ko05169 map03040 map03050 map05169
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

715

Amino Acids

80.46

Weight (kDa)

8.88

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 47 - 171 8.5e-06 DEAD/DEAH box helicase
Helicase_C PF00271 279 - 408 3.5e-12 Helicase conserved C-terminal domain
WHD_HA2 PF04408 471 - 500 2.1e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 501 - 562 7.8e-12 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 634 - 709 4.6e-22 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1480
AccI GTMKAC 1 cut(s) 763
AciI CCGC 7 cut(s) 33, 201, 330, 1246, 1306, 1878, 2077
AclWI GGATC 6 cut(s) 407, 440, 453, 1106, 1136, 1460
AcsI RAATTY 2 cut(s) 714, 1544
AcuI CTGAAG 2 cut(s) 1184, 1826
AcyI GRCGYC 2 cut(s) 75, 1477
AfaI GTAC 6 cut(s) 233, 540, 728, 804, 912, 1135
AfiI CCNNNNNNNGG 2 cut(s) 1151, 1684
AflII CTTAAG 1 cut(s) 1337
AhlI ACTAGT 1 cut(s) 2050
AjnI CCWGG 4 cut(s) 756, 862, 1090, 1114
AluBI AGCT 9 cut(s) 48, 97, 488, 746, 971, 1520, 1871, 1937, 2116
AluI AGCT 9 cut(s) 48, 97, 488, 746, 971, 1520, 1871, 1937, 2116
Alw26I GTCTC 3 cut(s) 910, 1067, 1766
AlwI GGATC 6 cut(s) 407, 440, 453, 1106, 1136, 1460
Ama87I CYCGRG 1 cut(s) 1949
AoxI GGCC 1 cut(s) 34
ApeKI GCWGC 5 cut(s) 45, 103, 293, 1913, 1934
ApoI RAATTY 2 cut(s) 714, 1544
AseI ATTAAT 1 cut(s) 1791
Asp700I GAANNNNTTC 1 cut(s) 1241
AspA2I CCTAGG 1 cut(s) 281
AspS9I GGNCC 2 cut(s) 859, 1672
AsuHPI GGTGA 2 cut(s) 356, 878
AsuII TTCGAA 1 cut(s) 1900
AvaI CYCGRG 1 cut(s) 1949
AvaII GGWCC 2 cut(s) 859, 1672
AvrII CCTAGG 1 cut(s) 281
BamHI GGATCC 1 cut(s) 445
BauI CACGAG 1 cut(s) 1815
BbsI GAAGAC 2 cut(s) 599, 1853
BbvCI CCTCAGC 1 cut(s) 321
BbvI GCAGC 5 cut(s) 57, 115, 280, 1925, 1946
BccI CCATC 4 cut(s) 410, 1003, 1028, 1661
BceAI ACGGC 1 cut(s) 29
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciT130I CCWGG 4 cut(s) 758, 864, 1092, 1116
BcoDI GTCTC 3 cut(s) 910, 1067, 1766
BcuI ACTAGT 1 cut(s) 2050
BfaI CTAG 9 cut(s) 282, 387, 815, 1320, 1433, 1499, 1635, 2051, 2088
BfrI CTTAAG 1 cut(s) 1337
BglII AGATCT 1 cut(s) 1732
BisI GCNGC 6 cut(s) 34, 46, 104, 294, 1914, 1935
BlnI CCTAGG 1 cut(s) 281
BlsI GCNGC 6 cut(s) 35, 47, 105, 295, 1915, 1936
BmcAI AGTACT 1 cut(s) 728
Bme1390I CCNGG 4 cut(s) 758, 864, 1092, 1116
Bme18I GGWCC 2 cut(s) 859, 1672
BmeT110I CYCGRG 1 cut(s) 1949
BmgT120I GGNCC 2 cut(s) 859, 1672
BmiI GGNNCC 4 cut(s) 447, 861, 1029, 1673
BmrFI CCNGG 4 cut(s) 758, 864, 1092, 1116
BmrI ACTGGG 1 cut(s) 530
BmsI GCATC 3 cut(s) 697, 808, 830
BmuI ACTGGG 1 cut(s) 530
BoxI GACNNNNGTC 1 cut(s) 512
BpiI GAAGAC 2 cut(s) 599, 1853
BpmI CTGGAG 1 cut(s) 1436
Bpu10I CCTNAGC 1 cut(s) 321
Bpu14I TTCGAA 1 cut(s) 1900
BpuEI CTTGAG 3 cut(s) 635, 661, 1298
Bsa29I ATCGAT 1 cut(s) 2094
BsaAI YACGTR 1 cut(s) 1133
BsaBI GATNNNNATC 1 cut(s) 450
BsaHI GRCGYC 2 cut(s) 75, 1477
BsaJI CCNNGG 8 cut(s) 281, 303, 661, 757, 862, 1115, 1837, 1950
BsaXI ACNNNNNCTCC 2 cut(s) 1409, 1439
Bsc4I CCNNNNNNNGG 2 cut(s) 1151, 1684
Bse118I RCCGGY 1 cut(s) 1024
Bse1I ACTGG 7 cut(s) 199, 525, 699, 892, 954, 1419, 1469
Bse3DI GCAATG 3 cut(s) 662, 1587, 1650
Bse8I GATNNNNATC 1 cut(s) 450
BseBI CCWGG 4 cut(s) 758, 864, 1092, 1116
BseCI ATCGAT 1 cut(s) 2094
BseDI CCNNGG 8 cut(s) 281, 303, 661, 757, 862, 1115, 1837, 1950
BseGI GGATG 6 cut(s) 382, 712, 753, 1888, 1996, 2073
BseJI GATNNNNATC 1 cut(s) 450
BseLI CCNNNNNNNGG 2 cut(s) 1151, 1684
BseMI GCAATG 3 cut(s) 662, 1587, 1650
BseMII CTCAG 8 cut(s) 236, 312, 419, 647, 1062, 1602, 1750, 1777
BseNI ACTGG 7 cut(s) 199, 525, 699, 892, 954, 1419, 1469
BseRI GAGGAG 2 cut(s) 869, 2112
BseXI GCAGC 5 cut(s) 57, 115, 280, 1925, 1946
BsgI GTGCAG 1 cut(s) 1236
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 1 cut(s) 36
BshVI ATCGAT 1 cut(s) 2094
BsiEI CGRYCG 1 cut(s) 126
BsiHKCI CYCGRG 1 cut(s) 1949
BsiSI CCGG 1 cut(s) 1025
BslFI GGGAC 1 cut(s) 1685
BslI CCNNNNNNNGG 2 cut(s) 1151, 1684
BsmAI GTCTC 3 cut(s) 910, 1067, 1766
BsmFI GGGAC 1 cut(s) 1685
BsmI GAATGC 1 cut(s) 332
BsnI GGCC 1 cut(s) 36
BsoBI CYCGRG 1 cut(s) 1949
Bsp119I TTCGAA 1 cut(s) 1900
Bsp1407I TGTACA 4 cut(s) 231, 538, 802, 910
BspACI CCGC 7 cut(s) 33, 201, 330, 1246, 1306, 1878, 2077
BspANI GGCC 1 cut(s) 36
BspCNI CTCAG 8 cut(s) 235, 313, 420, 646, 1063, 1601, 1749, 1776
BspDI ATCGAT 1 cut(s) 2094
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 4 cut(s) 447, 861, 1029, 1673
BspPI GGATC 6 cut(s) 407, 440, 453, 1106, 1136, 1460
BspQI GCTCTTC 1 cut(s) 1203
BspT104I TTCGAA 1 cut(s) 1900
BspTI CTTAAG 1 cut(s) 1337
BsrDI GCAATG 3 cut(s) 662, 1587, 1650
BsrFI RCCGGY 1 cut(s) 1024
BsrGI TGTACA 4 cut(s) 231, 538, 802, 910
BsrI ACTGG 7 cut(s) 199, 525, 699, 892, 954, 1419, 1469
BssAI RCCGGY 1 cut(s) 1024
BssECI CCNNGG 8 cut(s) 281, 303, 661, 757, 862, 1115, 1837, 1950
BssNI GRCGYC 2 cut(s) 75, 1477
BssSI CACGAG 1 cut(s) 1815
BssT1I CCWWGG 2 cut(s) 281, 661
Bst2BI CACGAG 1 cut(s) 1815
Bst2UI CCWGG 4 cut(s) 758, 864, 1092, 1116
Bst4CI ACNGT 5 cut(s) 304, 502, 768, 942, 1576
Bst6I CTCTTC 4 cut(s) 996, 1203, 1554, 1718
BstACI GRCGYC 2 cut(s) 75, 1477
BstAFI CTTAAG 1 cut(s) 1337
BstAUI TGTACA 4 cut(s) 231, 538, 802, 910
BstBAI YACGTR 1 cut(s) 1133
BstBI TTCGAA 1 cut(s) 1900
BstC8I GCNNGC 5 cut(s) 527, 1215, 1489, 1812, 2118
BstDEI CTNAG 9 cut(s) 222, 321, 428, 459, 633, 1071, 1588, 1736, 1763
BstDSI CCRYGG 1 cut(s) 303
BstF5I GGATG 6 cut(s) 382, 712, 753, 1888, 1996, 2073
BstMAI GTCTC 3 cut(s) 910, 1067, 1766
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 3 cut(s) 42, 103, 1640
BstNI CCWGG 4 cut(s) 758, 864, 1092, 1116
BstNSI RCATGY 3 cut(s) 610, 1814, 1891
BstPAI GACNNNNGTC 1 cut(s) 512
BstSCI CCNGG 4 cut(s) 756, 862, 1090, 1114
BstV1I GCAGC 5 cut(s) 57, 115, 280, 1925, 1946
BstV2I GAAGAC 2 cut(s) 599, 1853
BstX2I RGATCY 4 cut(s) 445, 1111, 1465, 1732
BstYI RGATCY 4 cut(s) 445, 1111, 1465, 1732
Bsu15I ATCGAT 1 cut(s) 2094
BsuRI GGCC 1 cut(s) 36
BsuTUI ATCGAT 1 cut(s) 2094
BtgI CCRYGG 1 cut(s) 303
BtsCI GGATG 6 cut(s) 382, 712, 753, 1888, 1996, 2073
BtsIMutI CAGTG 1 cut(s) 1412
Cac8I GCNNGC 5 cut(s) 527, 1215, 1489, 1812, 2118
CciI TCATGA 1 cut(s) 490
Cfr10I RCCGGY 1 cut(s) 1024
Cfr13I GGNCC 2 cut(s) 859, 1672
ClaI ATCGAT 1 cut(s) 2094
CseI GACGC 3 cut(s) 83, 131, 1905
Csp6I GTAC 6 cut(s) 232, 539, 727, 803, 911, 1134
CviQI GTAC 6 cut(s) 232, 539, 727, 803, 911, 1134
DdeI CTNAG 9 cut(s) 222, 321, 428, 459, 633, 1071, 1588, 1736, 1763
DraI TTTAAA 1 cut(s) 1941
Eam1104I CTCTTC 4 cut(s) 996, 1203, 1554, 1718
EarI CTCTTC 4 cut(s) 996, 1203, 1554, 1718
EciI GGCGGA 1 cut(s) 1235
Eco130I CCWWGG 2 cut(s) 281, 661
Eco47I GGWCC 2 cut(s) 859, 1672
Eco57I CTGAAG 2 cut(s) 1184, 1826
Eco88I CYCGRG 1 cut(s) 1949
EcoO109I RGGNCCY 1 cut(s) 859
EcoRI GAATTC 1 cut(s) 1544
EcoRII CCWGG 4 cut(s) 756, 862, 1090, 1114
EcoT14I CCWWGG 2 cut(s) 281, 661
EcoT22I ATGCAT 1 cut(s) 823
ErhI CCWWGG 2 cut(s) 281, 661
FalI AAGNNNNNCTT 2 cut(s) 918, 950
FaqI GGGAC 1 cut(s) 1685
FauI CCCGC 1 cut(s) 1871
FauNDI CATATG 1 cut(s) 849
FblI GTMKAC 1 cut(s) 763
Fnu4HI GCNGC 6 cut(s) 34, 46, 104, 294, 1914, 1935
FokI GGATG 6 cut(s) 389, 719, 740, 1895, 1983, 2060
Fsp4HI GCNGC 6 cut(s) 34, 46, 104, 294, 1914, 1935
FspBI CTAG 9 cut(s) 282, 387, 815, 1320, 1433, 1499, 1635, 2051, 2088
GluI GCNGC 6 cut(s) 34, 46, 104, 294, 1914, 1935
GsuI CTGGAG 1 cut(s) 1436
HaeIII GGCC 1 cut(s) 36
HapII CCGG 1 cut(s) 1025
HgaI GACGC 3 cut(s) 83, 131, 1905
Hin1I GRCGYC 2 cut(s) 75, 1477
HincII GTYRAC 2 cut(s) 764, 828
HindII GTYRAC 2 cut(s) 764, 828
HindIII AAGCTT 1 cut(s) 95
HpaI GTTAAC 1 cut(s) 828
HpaII CCGG 1 cut(s) 1025
HphI GGTGA 2 cut(s) 356, 878
Hpy166II GTNNAC 5 cut(s) 68, 751, 764, 828, 1457
Hpy188III TCNNGA 9 cut(s) 121, 491, 505, 815, 893, 1031, 1664, 1765, 1817
Hpy8I GTNNAC 5 cut(s) 68, 751, 764, 828, 1457
Hpy99I CGWCG 3 cut(s) 59, 77, 2005
HpyAV CCTTC 4 cut(s) 106, 660, 1398, 1502
HpyCH4III ACNGT 5 cut(s) 304, 502, 768, 942, 1576
HpyCH4IV ACGT 2 cut(s) 1132, 1477
HpyF10VI GCNNNNNNNGC 3 cut(s) 42, 103, 1640
HpyF3I CTNAG 9 cut(s) 222, 321, 428, 459, 633, 1071, 1588, 1736, 1763
HpySE526I ACGT 2 cut(s) 1132, 1477
Hsp92I GRCGYC 2 cut(s) 75, 1477
KspAI GTTAAC 1 cut(s) 828
LguI GCTCTTC 1 cut(s) 1203
LmnI GCTCC 5 cut(s) 1033, 1417, 1615, 1805, 2125
Lsp1109I GCAGC 5 cut(s) 57, 115, 280, 1925, 1946
LweI GCATC 3 cut(s) 697, 808, 830
MaeI CTAG 9 cut(s) 282, 387, 815, 1320, 1433, 1499, 1635, 2051, 2088
MaeII ACGT 2 cut(s) 1132, 1477
MaeIII GTNAC 9 cut(s) 271, 334, 356, 379, 942, 1081, 1310, 1817, 2106
MboII GAAGA 9 cut(s) 604, 908, 983, 1190, 1571, 1735, 1853, 1914, 1915
MfeI CAATTG 2 cut(s) 1421, 1549
MflI RGATCY 4 cut(s) 445, 1111, 1465, 1732
MlyI GAGTC 1 cut(s) 1604
MmeI TCCRAC 3 cut(s) 78, 594, 1453
Mph1103I ATGCAT 1 cut(s) 823
MroXI GAANNNNTTC 1 cut(s) 1241
MslI CAYNNNNRTG 3 cut(s) 798, 1815, 2130
MspA1I CMGCKG 1 cut(s) 33
MspCI CTTAAG 1 cut(s) 1337
MspI CCGG 1 cut(s) 1025
MspR9I CCNGG 4 cut(s) 758, 864, 1092, 1116
MunI CAATTG 2 cut(s) 1421, 1549
Mva1269I GAATGC 1 cut(s) 332
MvaI CCWGG 4 cut(s) 758, 864, 1092, 1116
MwoI GCNNNNNNNGC 3 cut(s) 42, 103, 1640
NdeI CATATG 1 cut(s) 849
NlaIV GGNNCC 4 cut(s) 447, 861, 1029, 1673
NmuCI GTSAC 4 cut(s) 379, 1081, 1310, 1817
NsiI ATGCAT 1 cut(s) 823
NspI RCATGY 3 cut(s) 610, 1814, 1891
NspV TTCGAA 1 cut(s) 1900
PaeI GCATGC 1 cut(s) 1814
PagI TCATGA 1 cut(s) 490
PciSI GCTCTTC 1 cut(s) 1203
PcsI WCGNNNNNNNCGW 1 cut(s) 477
PctI GAATGC 1 cut(s) 332
PdmI GAANNNNTTC 1 cut(s) 1241
PfeI GAWTC 9 cut(s) 61, 670, 844, 905, 1095, 1261, 1283, 1993, 2091
PkrI GCNGC 6 cut(s) 35, 47, 105, 295, 1915, 1936
Ple19I CGATCG 1 cut(s) 126
PleI GAGTC 1 cut(s) 1603
PpsI GAGTC 1 cut(s) 1603
Ppu21I YACGTR 1 cut(s) 1133
PpuMI RGGWCCY 1 cut(s) 859
PshAI GACNNNNGTC 1 cut(s) 512
PshBI ATTAAT 1 cut(s) 1791
Psp5II RGGWCCY 1 cut(s) 859
Psp6I CCWGG 4 cut(s) 756, 862, 1090, 1114
PspGI CCWGG 4 cut(s) 756, 862, 1090, 1114
PspN4I GGNNCC 4 cut(s) 447, 861, 1029, 1673
PspPI GGNCC 2 cut(s) 859, 1672
PspPPI RGGWCCY 1 cut(s) 859
PsuI RGATCY 4 cut(s) 445, 1111, 1465, 1732
PvuI CGATCG 1 cut(s) 126
RsaI GTAC 6 cut(s) 233, 540, 728, 804, 912, 1135
RsaNI GTAC 6 cut(s) 232, 539, 727, 803, 911, 1134
RseI CAYNNNNRTG 3 cut(s) 798, 1815, 2130
SalI GTCGAC 1 cut(s) 762
SapI GCTCTTC 1 cut(s) 1203
SatI GCNGC 6 cut(s) 34, 46, 104, 294, 1914, 1935
Sau96I GGNCC 2 cut(s) 859, 1672
ScaI AGTACT 1 cut(s) 728
SchI GAGTC 1 cut(s) 1604
ScrFI CCNGG 4 cut(s) 758, 864, 1092, 1116
SfaNI GCATC 3 cut(s) 697, 808, 830
SfuI TTCGAA 1 cut(s) 1900
SinI GGWCC 2 cut(s) 859, 1672
SmiMI CAYNNNNRTG 3 cut(s) 798, 1815, 2130
SmlI CTYRAG 4 cut(s) 640, 650, 1277, 1337
SmoI CTYRAG 4 cut(s) 640, 650, 1277, 1337
SpeI ACTAGT 1 cut(s) 2050
SphI GCATGC 1 cut(s) 1814
SsiI CCGC 7 cut(s) 33, 201, 330, 1246, 1306, 1878, 2077
SspI AATATT 2 cut(s) 1053, 1066
SspMI CTAG 9 cut(s) 282, 387, 815, 1320, 1433, 1499, 1635, 2051, 2088
StyD4I CCNGG 4 cut(s) 756, 862, 1090, 1114
StyI CCWWGG 2 cut(s) 281, 661
TaaI ACNGT 5 cut(s) 304, 502, 768, 942, 1576
TaiI ACGT 2 cut(s) 1135, 1480
TaqI TCGA 9 cut(s) 480, 763, 777, 1371, 1557, 1699, 1900, 2055, 2094
TatI WGTACW 5 cut(s) 231, 538, 726, 802, 910
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 9 cut(s) 61, 670, 844, 905, 1095, 1261, 1283, 1993, 2091
TscAI CASTG 1 cut(s) 1419
TseFI GTSAC 4 cut(s) 379, 1081, 1310, 1817
TseI GCWGC 5 cut(s) 45, 103, 293, 1913, 1934
Tsp45I GTSAC 4 cut(s) 379, 1081, 1310, 1817
TspDTI ATGAA 9 cut(s) 498, 836, 1366, 1661, 1767, 1777, 1853, 1985, 2061
TspGWI ACGGA 4 cut(s) 24, 1884, 1989, 1996
TspRI CASTG 1 cut(s) 1419
Vha464I CTTAAG 1 cut(s) 1337
VpaK11BI GGWCC 2 cut(s) 859, 1672
VspI ATTAAT 1 cut(s) 1791
XapI RAATTY 2 cut(s) 714, 1544
XbaI TCTAGA 1 cut(s) 814
XceI RCATGY 3 cut(s) 610, 1814, 1891
XmaJI CCTAGG 1 cut(s) 281
XmiI GTMKAC 1 cut(s) 763
XmnI GAANNNNTTC 1 cut(s) 1241
XspI CTAG 9 cut(s) 282, 387, 815, 1320, 1433, 1499, 1635, 2051, 2088
ZraI GACGTC 1 cut(s) 1478
ZrmI AGTACT 1 cut(s) 728
Zsp2I ATGCAT 1 cut(s) 823
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.