MD16G1060000.v1.1

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002639
Physical Location & Seq
Reverse (-)
4309322 .. 4314420
5099 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1060000.v1.1.491

Sequence Viewer

Length: 2151 bp
ATGGCTCAAGGCCAAGGACGGCCGAACAACTCCATGAAAAGCCCTAACCGCCAATTCTCCGCCGGCAACGGCAACGGCAACGTCGATGCCCTCGCCACCAAGAGGAAAGCGCTCGCGCAGCACAGGAAGTCCCTCCCAATCGCTTCTGTTGAAAATCGGCTAATCGAGGAGGTTCGGAAGCACGATACTTTGATTATTGTTGGCGAAACGGGAAGCGGAAAAACAACACAATTACCTCAGTTTTTGTTCAATGCTGGGTTTTCCGGGGACGGAAAAGTCATTGGGGTAACTCAACCTAGGCGTGTGGCTGCAATGACTGTGGCGAAGCGGGTTGCTGAGGAATGTGGCGGTAATTTGGGTCAGAAGGTTGGTTACTCCATTAGATTCGAGGACGTGACTTCTAGTTCAACACGGATCAAGTACATGACAGATGGATTGCTACTGAGGGAAGCACTATTGGATCCATATCTCTCTAGGTATTCAGTTATAATTGTTGATGAAGCTCATGAGAGAACTGTCCAGACAGATGTGCTACTAGGCTTGCTGAAAAATGTACAAGATGCAAGGTCAAAATCCCTCAACAACCTCCAGAATACTAAAAACCAGAAAACAAACGATGACATGCAGTCGAAAGAAAACGGTGCTCAGGGCTTCCGTTTTCTCAAGCAATACCAAGGAAGGACCCCGTTGAAGTTAATCATCATGTCCGCCAGCTTGGATGCAAAAATTTTCTCTGAGTACTTCGGTGGTGCAAGAGCTGTTCACATCCAAGGGCGACAGTTTCCCGTGGATATATTTTATACACATCATTCTATACAAGATTATCTTGATGCTTCGTTAACCACAATATTGCAGATTCACCTGGAGGAGGGCCCTGGTGATATACTTGTATTTTTGACGGGTCAAGAAGAAATTGAATCTGCAGAAAGACTTGTCAAAGAAAGACTTAAACAGTTACCAGAAAACAGTCAAAAACTAGTAGCTGTTCCTATATACTCATCTCTTTCATCAGAACATCAAATGCGAGTTTTTGCACCAGCCCCTCCTGGATTTCGTAAGGTAATATTGGCAACAAATATTGCTGAGACATCATTGACAATATCTGGAATCAAGTATGTTGTAGATCCTGGGTTTGTAAAAGCACGTTACTATGATCCAAACAAAGGGTTGGAATCATTGGTTGTTGTTCCAGTTTCAAAAGCACAGGCTCTTCAAAGGAGTGGGCGTGCTGGAAGAGAAGGACCGGGGAAATGCTTCCGCCTCTACCCAGAAGATCAATTTGGCAAACTTGAGGATTCGACAAAGCCAGAGATCAAGCGATGCAACCTCTCTAATGTCATTTTGCAACTTAAGGCGCTGGGTGTTGATGATATCACTGGGTTTGACTTCATAGAAAAACCGTCGAGGTCGGCTATTGTCAAATCACTGGAGCAATTGTTCGTACTGGGTGCTTTGACAGACGACTGCAAATTGTCAAATCCAGTTGGAATCCAAATGGCTCGCCTTCCCTTAGACCCTATTTATTCAAAGGCTCTAATCGTAGCAAGTGAATTCAATTGCCTGGAAGAGATGTTGATAAGTGTTGCAATGCTTTCGGTGGAATCAATATTTTTTAATCCCCGTGACAAAATAGAAGAGGTGAGAACTGCGCAGAAATCCTTTGCAAGTCCAGAGGGAGACCACCTCACTTTGGTTAATGTATATCGAGCATCCAATGAGTTCTTGGAGAAGAGATCAGGTCTTAGTAAAGAGAAGCGTGAAAAAGCCTTCCGGAAATGGTGCAAGGATAATTTCATTCATAGTCGCTCCTTGACAAATGCTCGTGACATTCACAGACAAATTCGAGGGCATGTTGAACAAATGGGTCTCCGTATCACCTCTTGTGGGGATGATGTGCTTCAGTTTTGCAGATGTCTTGCCGCTTCTTTCTTCCTCAATGCAGCTTTAAAGCAGCCTGATGGAACATACAGGGCTTTAGCAAATGGTGAGGTGGTTTCTATCCACCCGTCTTCTGTGTTAAGAAATAGACCAGAGTGCCTAATTTACAATGAACTGGTCGAAACTAGTAGAAAATTCATCCGCAACACAACTAGAATCGACTTTTTGTGGTTAACTGAGCTTGCTCCTCAATTCTATGCCATGCAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0001817 GO:0001819 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003723 GO:0003724 GO:0003725 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006356 GO:0006396 GO:0006412 GO:0006413 GO:0006518 GO:0006725 GO:0006807 GO:0008026 GO:0008134 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0023056 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032479 GO:0032481 GO:0032991 GO:0033613 GO:0034641 GO:0034645 GO:0042325 GO:0042327 GO:0042623 GO:0043021 GO:0043023 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0043408 GO:0043410 GO:0043484 GO:0043565 GO:0043603 GO:0043604 GO:0044087 GO:0044089 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045937 GO:0045943 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051090 GO:0051091 GO:0051092 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0060255 GO:0061702 GO:0065007 GO:0065009 GO:0070013 GO:0070035 GO:0071704 GO:0072559 GO:0080090 GO:0090304 GO:0097159 GO:0140098 GO:1900225 GO:1900227 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03040 ko03050 ko05169 map03040 map03050 map05169
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

717

Amino Acids

80.0

Weight (kDa)

9.12

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 53 - 174 1.5e-06 DEAD/DEAH box helicase
Helicase_C PF00271 282 - 414 1.3e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 475 - 504 4.1e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 505 - 564 5.8e-13 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 636 - 710 1e-19 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 488
AasI GACNNNNNNGTC 1 cut(s) 275
Acc16I TGCGCA 1 cut(s) 1650
AccII CGCG 1 cut(s) 116
AccIII TCCGGA 1 cut(s) 1770
AciI CCGC 9 cut(s) 49, 60, 216, 328, 348, 708, 1258, 1920, 2080
AclWI GGATC 5 cut(s) 422, 455, 468, 1118, 1148
AcoI YGGCCR 1 cut(s) 20
AcsI RAATTY 4 cut(s) 726, 1550, 1839, 2072
AcuI CTGAAG 1 cut(s) 1883
AfaI GTAC 4 cut(s) 422, 555, 740, 1443
AfeI AGCGCT 1 cut(s) 111
AfiI CCNNNNNNNGG 5 cut(s) 102, 868, 1163, 1690, 1884
AflII CTTAAG 1 cut(s) 1349
AhdI GACNNNNNGTC 1 cut(s) 625
AhlI ACTAGT 2 cut(s) 976, 2063
AjiI CACGTC 1 cut(s) 394
AjnI CCWGG 5 cut(s) 861, 874, 1045, 1126, 1560
AjuI GAANNNNNNNTTGG 2 cut(s) 1263, 1295
AloI GAACNNNNNNTCC 2 cut(s) 1421, 1453
AluBI AGCT 6 cut(s) 503, 714, 758, 983, 1943, 2119
AluI AGCT 6 cut(s) 503, 714, 758, 983, 1943, 2119
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 3 cut(s) 1079, 1671, 1871
AlwI GGATC 5 cut(s) 422, 455, 468, 1118, 1148
Aor13HI TCCGGA 1 cut(s) 1770
Aor51HI AGCGCT 1 cut(s) 111
AoxI GGCC 3 cut(s) 10, 20, 871
ApaI GGGCCC 1 cut(s) 875
ApeKI GCWGC 4 cut(s) 118, 308, 1940, 1951
ApoI RAATTY 4 cut(s) 726, 1550, 1839, 2072
Asp700I GAANNNNTTC 1 cut(s) 1253
AspA2I CCTAGG 1 cut(s) 296
AspLEI GCGC 4 cut(s) 112, 118, 1357, 1651
AspS9I GGNCC 4 cut(s) 681, 871, 872, 1241
AsuC2I CCSGG 2 cut(s) 265, 1245
AsuHPI GGTGA 5 cut(s) 851, 890, 1651, 1867, 1997
AvaII GGWCC 2 cut(s) 681, 1241
AvrII CCTAGG 1 cut(s) 296
BaeGI GKGCMC 1 cut(s) 875
BamHI GGATCC 1 cut(s) 460
BanII GRGCYC 1 cut(s) 875
BarI GAAGNNNNNNTAC 2 cut(s) 356, 388
BauI CACGAG 1 cut(s) 1821
BbsI GAAGAC 1 cut(s) 2001
Bbv12I GWGCWC 1 cut(s) 646
BbvCI CCTCAGC 1 cut(s) 336
BbvI GCAGC 4 cut(s) 130, 295, 1952, 1963
BccI CCATC 2 cut(s) 425, 1952
BceAI ACGGC 3 cut(s) 35, 85, 91
BcgI CGANNNNNNTGC 2 cut(s) 1575, 1609
BciT130I CCWGG 5 cut(s) 863, 876, 1047, 1128, 1562
BcnI CCSGG 2 cut(s) 265, 1245
BcoDI GTCTC 3 cut(s) 1079, 1671, 1871
BcuI ACTAGT 2 cut(s) 976, 2063
BfaI CTAG 7 cut(s) 297, 402, 474, 536, 977, 2064, 2091
BfmI CTRYAG 1 cut(s) 921
BfoI RGCGCY 2 cut(s) 113, 1358
BfrI CTTAAG 1 cut(s) 1349
BisI GCNGC 5 cut(s) 119, 309, 1920, 1941, 1952
BlnI CCTAGG 1 cut(s) 296
BlsI GCNGC 5 cut(s) 120, 310, 1921, 1942, 1953
BmcAI AGTACT 1 cut(s) 740
Bme1390I CCNGG 7 cut(s) 265, 863, 876, 1047, 1128, 1245, 1562
Bme18I GGWCC 2 cut(s) 681, 1241
BmeRI GACNNNNNGTC 1 cut(s) 625
BmgBI CACGTC 1 cut(s) 394
BmgT120I GGNCC 4 cut(s) 681, 871, 872, 1241
BmiI GGNNCC 3 cut(s) 462, 683, 873
BmrFI CCNGG 7 cut(s) 265, 863, 876, 1047, 1128, 1245, 1562
BmrI ACTGGG 2 cut(s) 1386, 1454
BmsI GCATC 6 cut(s) 76, 550, 709, 820, 1310, 1718
BmuI ACTGGG 2 cut(s) 1386, 1454
BpiI GAAGAC 1 cut(s) 2001
BplI GAGNNNNNCTC 2 cut(s) 1668, 1700
BpmI CTGGAG 3 cut(s) 572, 884, 1448
Bpu10I CCTNAGC 2 cut(s) 336, 645
BpuEI CTTGAG 2 cut(s) 647, 1310
BpuMI CCSGG 2 cut(s) 265, 1245
BsaBI GATNNNNATC 1 cut(s) 465
BsaI GGTCTC 2 cut(s) 1671, 1871
BsaJI CCNNGG 9 cut(s) 13, 264, 296, 673, 769, 786, 874, 1127, 1244
BsaWI WCCGGW 1 cut(s) 1770
BsaXI ACNNNNNCTCC 4 cut(s) 1210, 1240, 1421, 1451
Bsc4I CCNNNNNNNGG 5 cut(s) 102, 868, 1163, 1690, 1884
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 6 cut(s) 1190, 1381, 1431, 1449, 1481, 2058
Bse3DI GCAATG 2 cut(s) 318, 1593
Bse8I GATNNNNATC 1 cut(s) 465
BseAI TCCGGA 1 cut(s) 1770
BseBI CCWGG 5 cut(s) 863, 876, 1047, 1128, 1562
BseDI CCNNGG 9 cut(s) 13, 264, 296, 673, 769, 786, 874, 1127, 1244
BseGI GGATG 5 cut(s) 724, 765, 1709, 1894, 2076
BseJI GATNNNNATC 1 cut(s) 465
BseLI CCNNNNNNNGG 5 cut(s) 102, 868, 1163, 1690, 1884
BseMI GCAATG 2 cut(s) 318, 1593
BseMII CTCAG 7 cut(s) 251, 327, 434, 659, 726, 1074, 2106
BseNI ACTGG 6 cut(s) 1190, 1381, 1431, 1449, 1481, 2058
BseRI GAGGAG 3 cut(s) 182, 881, 2115
BseSI GKGCMC 1 cut(s) 875
BseX3I CGGCCG 1 cut(s) 20
BseXI GCAGC 4 cut(s) 130, 295, 1952, 1963
BseYI CCCAGC 2 cut(s) 254, 1357
Bsh1236I CGCG 1 cut(s) 116
Bsh1285I CGRYCG 1 cut(s) 23
BshFI GGCC 3 cut(s) 12, 22, 873
BsiEI CGRYCG 1 cut(s) 23
BsiHKAI GWGCWC 1 cut(s) 646
BsiSI CCGG 4 cut(s) 63, 264, 1244, 1771
BslFI GGGAC 2 cut(s) 115, 281
BslI CCNNNNNNNGG 5 cut(s) 102, 868, 1163, 1690, 1884
BsmAI GTCTC 3 cut(s) 1079, 1671, 1871
BsmFI GGGAC 2 cut(s) 115, 281
BsnI GGCC 3 cut(s) 12, 22, 873
Bso31I GGTCTC 2 cut(s) 1671, 1871
Bsp120I GGGCCC 1 cut(s) 871
Bsp1286I GDGCHC 2 cut(s) 646, 875
Bsp13I TCCGGA 1 cut(s) 1770
Bsp1407I TGTACA 1 cut(s) 553
Bsp143I GATC 7 cut(s) 414, 460, 1123, 1153, 1273, 1311, 1733
BspACI CCGC 9 cut(s) 49, 60, 216, 328, 348, 708, 1258, 1920, 2080
BspANI GGCC 3 cut(s) 12, 22, 873
BspCNI CTCAG 7 cut(s) 250, 328, 435, 658, 727, 1075, 2107
BspEI TCCGGA 1 cut(s) 1770
BspFNI CGCG 1 cut(s) 116
BspHI TCATGA 1 cut(s) 505
BspLI GGNNCC 3 cut(s) 462, 683, 873
BspMAI CTGCAG 1 cut(s) 925
BspPI GGATC 5 cut(s) 422, 455, 468, 1118, 1148
BspQI GCTCTTC 1 cut(s) 1215
BspTI CTTAAG 1 cut(s) 1349
BspTNI GGTCTC 2 cut(s) 1671, 1871
BsrDI GCAATG 2 cut(s) 318, 1593
BsrFI RCCGGY 1 cut(s) 62
BsrGI TGTACA 1 cut(s) 553
BsrI ACTGG 6 cut(s) 1190, 1381, 1431, 1449, 1481, 2058
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 9 cut(s) 13, 264, 296, 673, 769, 786, 874, 1127, 1244
BssMI GATC 7 cut(s) 414, 460, 1123, 1153, 1273, 1311, 1733
BssSI CACGAG 1 cut(s) 1821
BssT1I CCWWGG 4 cut(s) 13, 296, 673, 769
Bst2BI CACGAG 1 cut(s) 1821
Bst2UI CCWGG 5 cut(s) 863, 876, 1047, 1128, 1562
Bst4CI ACNGT 7 cut(s) 319, 517, 641, 780, 954, 968, 1401
Bst6I CTCTTC 5 cut(s) 1215, 1228, 1560, 1629, 1724
BstAFI CTTAAG 1 cut(s) 1349
BstAUI TGTACA 1 cut(s) 553
BstC8I GCNNGC 7 cut(s) 64, 114, 542, 712, 1227, 1501, 2121
BstDEI CTNAG 9 cut(s) 237, 336, 443, 645, 735, 1083, 1510, 1742, 2115
BstDSI CCRYGG 1 cut(s) 786
BstENI CCTNNNNNAGG 1 cut(s) 866
BstF5I GGATG 5 cut(s) 724, 765, 1709, 1894, 2076
BstFNI CGCG 1 cut(s) 116
BstH2I RGCGCY 2 cut(s) 113, 1358
BstHHI GCGC 4 cut(s) 112, 118, 1357, 1651
BstKTI GATC 7 cut(s) 417, 463, 1126, 1156, 1276, 1314, 1736
BstMAI GTCTC 3 cut(s) 1079, 1671, 1871
BstMBI GATC 7 cut(s) 414, 460, 1123, 1153, 1273, 1311, 1733
BstMCI CGRYCG 1 cut(s) 23
BstMWI GCNNNNNNNGC 2 cut(s) 48, 118
BstNI CCWGG 5 cut(s) 863, 876, 1047, 1128, 1562
BstNSI RCATGY 2 cut(s) 625, 1853
BstSCI CCNGG 7 cut(s) 263, 861, 874, 1045, 1126, 1243, 1560
BstSFI CTRYAG 1 cut(s) 921
BstSLI GKGCMC 1 cut(s) 875
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 4 cut(s) 130, 295, 1952, 1963
BstV2I GAAGAC 1 cut(s) 2001
BstX2I RGATCY 2 cut(s) 460, 1123
BstYI RGATCY 2 cut(s) 460, 1123
BstZI CGGCCG 1 cut(s) 20
BsuRI GGCC 3 cut(s) 12, 22, 873
BtgI CCRYGG 1 cut(s) 786
BtgZI GCGATG 1 cut(s) 1333
BtrI CACGTC 1 cut(s) 394
BtsCI GGATG 5 cut(s) 724, 765, 1709, 1894, 2076
BtsIMutI CAGTG 2 cut(s) 1374, 1424
Cac8I GCNNGC 7 cut(s) 64, 114, 542, 712, 1227, 1501, 2121
CciI TCATGA 1 cut(s) 505
CfoI GCGC 4 cut(s) 112, 118, 1357, 1651
Cfr10I RCCGGY 1 cut(s) 62
Cfr13I GGNCC 4 cut(s) 681, 871, 872, 1241
Csp6I GTAC 4 cut(s) 421, 554, 739, 1442
CspCI CAANNNNNGTGG 2 cut(s) 300, 335
CviAII CATG 7 cut(s) 34, 424, 506, 622, 703, 1850, 2140
CviQI GTAC 4 cut(s) 421, 554, 739, 1442
DdeI CTNAG 9 cut(s) 237, 336, 443, 645, 735, 1083, 1510, 1742, 2115
DpnI GATC 7 cut(s) 416, 462, 1125, 1155, 1275, 1313, 1735
DpnII GATC 7 cut(s) 414, 460, 1123, 1153, 1273, 1311, 1733
DraI TTTAAA 1 cut(s) 1947
DrdI GACNNNNNNGTC 1 cut(s) 275
DriI GACNNNNNGTC 1 cut(s) 625
DseDI GACNNNNNNGTC 1 cut(s) 275
EaeI YGGCCR 1 cut(s) 20
EagI CGGCCG 1 cut(s) 20
Eam1104I CTCTTC 5 cut(s) 1215, 1228, 1560, 1629, 1724
Eam1105I GACNNNNNGTC 1 cut(s) 625
EarI CTCTTC 5 cut(s) 1215, 1228, 1560, 1629, 1724
EciI GGCGGA 3 cut(s) 49, 697, 1247
EclXI CGGCCG 1 cut(s) 20
Eco130I CCWWGG 4 cut(s) 13, 296, 673, 769
Eco24I GRGCYC 1 cut(s) 875
Eco31I GGTCTC 2 cut(s) 1671, 1871
Eco32I GATATC 1 cut(s) 1372
Eco47I GGWCC 2 cut(s) 681, 1241
Eco47III AGCGCT 1 cut(s) 111
Eco52I CGGCCG 1 cut(s) 20
Eco57I CTGAAG 1 cut(s) 1883
EcoNI CCTNNNNNAGG 1 cut(s) 866
EcoO109I RGGNCCY 3 cut(s) 681, 871, 872
EcoRI GAATTC 1 cut(s) 1550
EcoRII CCWGG 5 cut(s) 861, 874, 1045, 1126, 1560
EcoRV GATATC 1 cut(s) 1372
EcoT14I CCWWGG 4 cut(s) 13, 296, 673, 769
EcoT38I GRGCYC 1 cut(s) 875
ErhI CCWWGG 4 cut(s) 13, 296, 673, 769
FaeI CATG 7 cut(s) 37, 427, 509, 625, 706, 1853, 2143
FalI AAGNNNNNCTT 4 cut(s) 810, 842, 930, 962
FaqI GGGAC 2 cut(s) 115, 281
FatI CATG 7 cut(s) 33, 423, 505, 621, 702, 1849, 2139
FauI CCCGC 1 cut(s) 321
Fnu4HI GCNGC 5 cut(s) 119, 309, 1920, 1941, 1952
FokI GGATG 5 cut(s) 731, 752, 1696, 1901, 2063
FriOI GRGCYC 1 cut(s) 875
Fsp4HI GCNGC 5 cut(s) 119, 309, 1920, 1941, 1952
FspBI CTAG 7 cut(s) 297, 402, 474, 536, 977, 2064, 2091
FspI TGCGCA 1 cut(s) 1650
GlaI GCGC 4 cut(s) 111, 117, 1356, 1650
GluI GCNGC 5 cut(s) 119, 309, 1920, 1941, 1952
GsaI CCCAGC 2 cut(s) 258, 1361
GsuI CTGGAG 3 cut(s) 572, 884, 1448
HaeII RGCGCY 2 cut(s) 113, 1358
HaeIII GGCC 3 cut(s) 12, 22, 873
HapII CCGG 4 cut(s) 63, 264, 1244, 1771
HhaI GCGC 4 cut(s) 112, 118, 1357, 1651
Hin1II CATG 7 cut(s) 37, 427, 509, 625, 706, 1853, 2143
Hin6I GCGC 4 cut(s) 110, 116, 1355, 1649
HinP1I GCGC 4 cut(s) 110, 116, 1355, 1649
HincII GTYRAC 2 cut(s) 840, 2112
HindII GTYRAC 2 cut(s) 840, 2112
HinfI GANTC 9 cut(s) 384, 856, 917, 1107, 1172, 1295, 1488, 1601, 2094
HpaI GTTAAC 2 cut(s) 840, 2112
HpaII CCGG 4 cut(s) 63, 264, 1244, 1771
HphI GGTGA 5 cut(s) 851, 890, 1651, 1867, 1997
Hpy166II GTNNAC 3 cut(s) 763, 840, 2112
Hpy188I TCNGA 4 cut(s) 177, 363, 736, 1012
Hpy188III TCNNGA 9 cut(s) 506, 520, 589, 827, 905, 1104, 1670, 1771, 1823
Hpy8I GTNNAC 3 cut(s) 763, 840, 2112
Hpy99I CGWCG 2 cut(s) 86, 1405
HpyAV CCTTC 5 cut(s) 358, 672, 1232, 1514, 1777
HpyCH4III ACNGT 7 cut(s) 319, 517, 641, 780, 954, 968, 1401
HpyCH4IV ACGT 3 cut(s) 81, 393, 1144
HpyF10VI GCNNNNNNNGC 2 cut(s) 48, 118
HpyF3I CTNAG 9 cut(s) 237, 336, 443, 645, 735, 1083, 1510, 1742, 2115
HpySE526I ACGT 3 cut(s) 81, 393, 1144
Hsp92II CATG 7 cut(s) 37, 427, 509, 625, 706, 1853, 2143
HspAI GCGC 4 cut(s) 110, 116, 1355, 1649
Kpn2I TCCGGA 1 cut(s) 1770
KroI GCCGGC 1 cut(s) 62
KroNI GCCGGC 1 cut(s) 64
KspAI GTTAAC 2 cut(s) 840, 2112
Kzo9I GATC 7 cut(s) 414, 460, 1123, 1153, 1273, 1311, 1733
LguI GCTCTTC 1 cut(s) 1215
LmnI GCTCC 3 cut(s) 1429, 1811, 2128
Lsp1109I GCAGC 4 cut(s) 130, 295, 1952, 1963
LweI GCATC 6 cut(s) 76, 550, 709, 820, 1310, 1718
MaeI CTAG 7 cut(s) 297, 402, 474, 536, 977, 2064, 2091
MaeII ACGT 3 cut(s) 81, 393, 1144
MaeIII GTNAC 7 cut(s) 286, 371, 394, 954, 1145, 1622, 1823
MalI GATC 7 cut(s) 416, 462, 1125, 1155, 1275, 1313, 1735
MboI GATC 7 cut(s) 414, 460, 1123, 1153, 1273, 1311, 1733
MboII GAAGA 9 cut(s) 920, 1202, 1245, 1283, 1577, 1646, 1741, 1921, 2001
MfeI CAATTG 2 cut(s) 1433, 1555
MflI RGATCY 2 cut(s) 460, 1123
MhlI GDGCHC 2 cut(s) 646, 875
MmeI TCCRAC 2 cut(s) 1149, 1465
MroI TCCGGA 1 cut(s) 1770
MroNI GCCGGC 1 cut(s) 62
MroXI GAANNNNTTC 1 cut(s) 1253
MseI TTAA 9 cut(s) 695, 839, 948, 1350, 1614, 1695, 1946, 2018, 2111
MspCI CTTAAG 1 cut(s) 1349
MspI CCGG 4 cut(s) 63, 264, 1244, 1771
MspR9I CCNGG 7 cut(s) 265, 863, 876, 1047, 1128, 1245, 1562
MunI CAATTG 2 cut(s) 1433, 1555
MvaI CCWGG 5 cut(s) 863, 876, 1047, 1128, 1562
MvnI CGCG 1 cut(s) 116
MwoI GCNNNNNNNGC 2 cut(s) 48, 118
NaeI GCCGGC 1 cut(s) 64
NciI CCSGG 2 cut(s) 265, 1245
NdeII GATC 7 cut(s) 414, 460, 1123, 1153, 1273, 1311, 1733
NgoMIV GCCGGC 1 cut(s) 62
NlaIII CATG 7 cut(s) 37, 427, 509, 625, 706, 1853, 2143
NlaIV GGNNCC 3 cut(s) 462, 683, 873
NmuCI GTSAC 3 cut(s) 394, 1622, 1823
NsbI TGCGCA 1 cut(s) 1650
NspI RCATGY 2 cut(s) 625, 1853
PagI TCATGA 1 cut(s) 505
PciSI GCTCTTC 1 cut(s) 1215
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PdiI GCCGGC 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 1253
PfeI GAWTC 9 cut(s) 384, 856, 917, 1107, 1172, 1295, 1488, 1601, 2094
PfoI TCCNGGA 1 cut(s) 1045
PkrI GCNGC 5 cut(s) 120, 310, 1921, 1942, 1953
PpuMI RGGWCCY 1 cut(s) 681
PsiI TTATAA 1 cut(s) 488
Psp5II RGGWCCY 1 cut(s) 681
Psp6I CCWGG 5 cut(s) 861, 874, 1045, 1126, 1560
PspFI CCCAGC 2 cut(s) 254, 1357
PspGI CCWGG 5 cut(s) 861, 874, 1045, 1126, 1560
PspN4I GGNNCC 3 cut(s) 462, 683, 873
PspOMI GGGCCC 1 cut(s) 871
PspPI GGNCC 4 cut(s) 681, 871, 872, 1241
PspPPI RGGWCCY 1 cut(s) 681
PstI CTGCAG 1 cut(s) 925
PsuI RGATCY 2 cut(s) 460, 1123
RsaI GTAC 4 cut(s) 422, 555, 740, 1443
RsaNI GTAC 4 cut(s) 421, 554, 739, 1442
SapI GCTCTTC 1 cut(s) 1215
SaqAI TTAA 9 cut(s) 695, 839, 948, 1350, 1614, 1695, 1946, 2018, 2111
SatI GCNGC 5 cut(s) 119, 309, 1920, 1941, 1952
Sau3AI GATC 7 cut(s) 414, 460, 1123, 1153, 1273, 1311, 1733
Sau96I GGNCC 4 cut(s) 681, 871, 872, 1241
ScaI AGTACT 1 cut(s) 740
ScrFI CCNGG 7 cut(s) 265, 863, 876, 1047, 1128, 1245, 1562
SduI GDGCHC 2 cut(s) 646, 875
SfaNI GCATC 6 cut(s) 76, 550, 709, 820, 1310, 1718
SfcI CTRYAG 1 cut(s) 921
SinI GGWCC 2 cut(s) 681, 1241
SmlI CTYRAG 4 cut(s) 6, 662, 1289, 1349
SmoI CTYRAG 4 cut(s) 6, 662, 1289, 1349
SpeI ACTAGT 2 cut(s) 976, 2063
SsiI CCGC 9 cut(s) 49, 60, 216, 328, 348, 708, 1258, 1920, 2080
SspI AATATT 4 cut(s) 849, 1065, 1078, 1608
SspMI CTAG 7 cut(s) 297, 402, 474, 536, 977, 2064, 2091
StyD4I CCNGG 7 cut(s) 263, 861, 874, 1045, 1126, 1243, 1560
StyI CCWWGG 4 cut(s) 13, 296, 673, 769
TaaI ACNGT 7 cut(s) 319, 517, 641, 780, 954, 968, 1401
TaiI ACGT 3 cut(s) 84, 396, 1147
TatI WGTACW 3 cut(s) 420, 553, 738
TauI GCSGC 1 cut(s) 1922
TfiI GAWTC 9 cut(s) 384, 856, 917, 1107, 1172, 1295, 1488, 1601, 2094
Tru1I TTAA 9 cut(s) 695, 839, 948, 1350, 1614, 1695, 1946, 2018, 2111
Tru9I TTAA 9 cut(s) 695, 839, 948, 1350, 1614, 1695, 1946, 2018, 2111
TscAI CASTG 2 cut(s) 1381, 1431
TseFI GTSAC 3 cut(s) 394, 1622, 1823
TseI GCWGC 4 cut(s) 118, 308, 1940, 1951
Tsp45I GTSAC 3 cut(s) 394, 1622, 1823
TspDTI ATGAA 8 cut(s) 50, 513, 996, 1378, 1783, 1787, 2064, 2065
TspGWI ACGGA 4 cut(s) 285, 427, 644, 1859
TspRI CASTG 2 cut(s) 1381, 1431
Vha464I CTTAAG 1 cut(s) 1349
VpaK11BI GGWCC 2 cut(s) 681, 1241
XagI CCTNNNNNAGG 1 cut(s) 866
XapI RAATTY 4 cut(s) 726, 1550, 1839, 2072
XceI RCATGY 2 cut(s) 625, 1853
XcmI CCANNNNNNNNNTGG 1 cut(s) 1720
XmaJI CCTAGG 1 cut(s) 296
XmnI GAANNNNTTC 1 cut(s) 1253
XspI CTAG 7 cut(s) 297, 402, 474, 536, 977, 2064, 2091
ZrmI AGTACT 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.