Rroxscaffold_5G00379250

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002639
Physical Location & Seq
Reverse (-)
59677275 .. 59685505
8231 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00379250.1

Sequence Viewer

Length: 2025 bp
ATGGCTCACGGACGGCTCAATAACTCCACCAACGGCCTTAAACGCAGCTTCTCCGACGAGAATCGTAAGCCCGACGCCTTTGCCAAGAGGCAGAAGCTTTCGCAGCACAGGAAGGCTCTCCCGATCGCTTCTGTTGAGAAGCGTCTCGTAGAGGAAGTTAGAAAGCATGATACTTTGATTATTGTTGGCGAAACTGGAAGCGGAAAAACAACACAATTACCTCAGTTTTTGTACAATGCTGGATTTTGTGGGTATGGGAAAGTCATTGGGGTAACTCAGCCCAGGCGTGTGGCTGCTATGACTGTGGCGAAACGGGTTGCTGAGGAATGCGGTGTTACTTTGGGTGAAAAGGTTGGTTACGCTGTCAGATTTGAGGATGTGACCTCTAGTTCAACAAGGGTCAAGTATATGACTGATGGATTGCTACTGAGGGAAGCACTATTGGATCCATACCTCTCTAAGTATTCAGTCGTTATTGTCGATGAAGCTCATGAGAGAACTGTCCAGACTGATGTCTTACTGGGCTTGCTTAAAAATGTACAAAATGCAAGGTCAAAAGTTGTCAATAACCACCAAGATACAAAAAACACAAAGACAAATAATGACATGCAGTTGGATAAAGAAAATGGTTCTCAGTCCTTGAGTTTTCTCAAGCAATGCCAAGGAAGGAATCCATTGAAGTTAATTATCATGTCTGCCAGTTTGGATGCACGAATTTTCTCCGAGTACTTCGGCGGTGCAAGAGCTGTTCACATCCAAGGTCGACAGTTTCCTGTCGATATATATTATACTCATCACAATGTACAAGATTATCTAGATGCATCGTTAACTGCCATATTTCAGATTCATATGGAGGAGGACCCTGGTGATATACTTGTCTTTTTGACTGGTCAAGAAGAAATTGAATCTGTGCAGAGACTTGTCAAAGAAAGACTTAAACAGTTACCTGAAACCAGTCAAAAACTTGTAGCTGTTCCTATATTCTCATCTCTTCCATCAGAACAGCAAATGCAAGTGTTTGCACCGGCTCCTGATGGATTTCGTAAGGTAATATTGGCAACGAATATTGCTGAGACATCTGTGACAATACCTGGAATCAAGTATGTAATAGATCCTGGGTTTGTAAAAGCACGTACCTATGATCCGAATAAAGGGTTATCAAGTTTGAACGTTATTCCAATTTCAAAAGCACAGGCTCTTCAGAGAAGGTATGCGATGCTGTGGGCGTGCAGGAGAGAAAGATCAGGGAAGTGCTTCCGCCTCTACCCAGAGAATCAATTTGAGAAACTTGAGGATTCAACAAAGCCAGAGATCAAGCGGTGTGACCTCTCTAGTATAATTTTGCAACTTAAGGTTCTGGGTGTTGATGATGTCATTGGGTTCGACTTCATAGATAAACCTTCAAGGTCGGCTATTGTCAAATCACTGGAGCAATTGTATGTGCTAGGTGCTTTAACAGATGAGTGTAAACTATCAGATCCAGTTGGACGTCAAATGGCAAGCCTTCCCCTAGACCCTATTTTCTCAAAAGCTCTAATCCGAGCAAGCGAAACTGAATTCAATTGCCTGGAAGAGATGTTGATAACTGTTGCAATGCTCTCAGTTGAGTCCATATTTTTTGCTCCCCGTGAGAAATTGAACGAGGCTAGAACTGCAATGAAATGCTTTGCAAGTCCAGATGGGGACCACCTCACTTTGGTTAATGTATATCGAGCGTTTAATGAGTTTTCAGAGAAGAGATCAGATCTCAGTAAAGAGAAACATGAAAAAAGTCTCAGGAAATGGTGCAAGGACAATTTCATTAATAGTCGCTCCCTGAAGCATGCTCGTGACATTCACAGGGCTTTAGCAAATGGTCAGGTGGTTCGGATTCATCCGTCGTCCGTGTTGTTCCATGAGAAACCAGAGTGTATAGTTTTTAATGAACTAGTCGAAACTAATCATAAATACATCCGCAACACAACTAGAATCGATTATTTATGGTTACATGAGCTTGCTCCTCATTTCTATGCCATGCAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0001817 GO:0001819 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003723 GO:0003724 GO:0003725 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006356 GO:0006396 GO:0006412 GO:0006413 GO:0006518 GO:0006725 GO:0006807 GO:0008026 GO:0008134 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0023056 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032479 GO:0032481 GO:0032991 GO:0033613 GO:0034641 GO:0034645 GO:0042325 GO:0042327 GO:0042623 GO:0043021 GO:0043023 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0043408 GO:0043410 GO:0043484 GO:0043565 GO:0043603 GO:0043604 GO:0044087 GO:0044089 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045937 GO:0045943 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051090 GO:0051091 GO:0051092 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0060255 GO:0061702 GO:0065007 GO:0065009 GO:0070013 GO:0070035 GO:0071704 GO:0072559 GO:0080090 GO:0090304 GO:0097159 GO:0140098 GO:1900225 GO:1900227 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03040 ko03050 ko05169 map03040 map03050 map05169
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

674

Amino Acids

76.34

Weight (kDa)

8.91

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 279 - 374 9.3e-09 Helicase conserved C-terminal domain
WHD_HA2 PF04408 475 - 504 2e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 505 - 566 7.2e-12 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 613 - 668 9.3e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1492
AccI GTMKAC 1 cut(s) 763
AciI CCGC 6 cut(s) 201, 330, 735, 1258, 1318, 1954
AclI AACGTT 1 cut(s) 1170
AclWI GGATC 5 cut(s) 440, 453, 1106, 1136, 1472
AcsI RAATTY 2 cut(s) 714, 1556
AcuI CTGAAG 2 cut(s) 1184, 1838
AcyI GRCGYC 2 cut(s) 75, 1489
AfaI GTAC 5 cut(s) 233, 540, 728, 804, 1135
AfiI CCNNNNNNNGG 2 cut(s) 1151, 1696
AflII CTTAAG 1 cut(s) 1349
AgsI TTSAA 9 cut(s) 393, 679, 905, 1168, 1185, 1299, 1404, 1561, 1639
AhlI ACTAGT 1 cut(s) 1927
AjnI CCWGG 5 cut(s) 281, 862, 1090, 1114, 1566
AluBI AGCT 7 cut(s) 48, 97, 488, 746, 971, 1532, 1993
AluI AGCT 7 cut(s) 48, 97, 488, 746, 971, 1532, 1993
Alw26I GTCTC 4 cut(s) 149, 910, 1067, 1778
AlwI GGATC 5 cut(s) 440, 453, 1106, 1136, 1472
AoxI GGCC 1 cut(s) 34
ApeKI GCWGC 3 cut(s) 45, 103, 293
ApoI RAATTY 2 cut(s) 714, 1556
AseI ATTAAT 1 cut(s) 1803
Asp700I GAANNNNTTC 1 cut(s) 1253
AspS9I GGNCC 2 cut(s) 859, 1684
AsuHPI GGTGA 2 cut(s) 356, 878
AvaII GGWCC 2 cut(s) 859, 1684
BamHI GGATCC 1 cut(s) 445
BauI CACGAG 1 cut(s) 1827
BbvCI CCTCAGC 1 cut(s) 321
BbvI GCAGC 3 cut(s) 57, 115, 280
BccI CCATC 4 cut(s) 410, 1003, 1028, 1673
BceAI ACGGC 2 cut(s) 29, 49
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciT130I CCWGG 5 cut(s) 283, 864, 1092, 1116, 1568
BcoDI GTCTC 4 cut(s) 149, 910, 1067, 1778
BcuI ACTAGT 1 cut(s) 1927
BfaI CTAG 8 cut(s) 387, 815, 1332, 1445, 1511, 1647, 1928, 1965
BfrI CTTAAG 1 cut(s) 1349
BglII AGATCT 1 cut(s) 1744
BisI GCNGC 3 cut(s) 46, 104, 294
BlsI GCNGC 3 cut(s) 47, 105, 295
BmcAI AGTACT 1 cut(s) 728
Bme1390I CCNGG 5 cut(s) 283, 864, 1092, 1116, 1568
Bme18I GGWCC 2 cut(s) 859, 1684
BmgT120I GGNCC 2 cut(s) 859, 1684
BmiI GGNNCC 4 cut(s) 447, 861, 1029, 1685
BmrFI CCNGG 5 cut(s) 283, 864, 1092, 1116, 1568
BmrI ACTGGG 1 cut(s) 530
BmsI GCATC 4 cut(s) 697, 808, 830, 1206
BmuI ACTGGG 1 cut(s) 530
BoxI GACNNNNGTC 1 cut(s) 512
BpmI CTGGAG 1 cut(s) 1448
Bpu10I CCTNAGC 1 cut(s) 321
BpuEI CTTGAG 3 cut(s) 635, 661, 1310
Bsa29I ATCGAT 1 cut(s) 1971
BsaAI YACGTR 1 cut(s) 1133
BsaHI GRCGYC 2 cut(s) 75, 1489
BsaJI CCNNGG 5 cut(s) 281, 661, 757, 862, 1115
BsaXI ACNNNNNCTCC 2 cut(s) 1421, 1451
Bsc4I CCNNNNNNNGG 2 cut(s) 1151, 1696
Bse118I RCCGGY 1 cut(s) 1024
Bse1I ACTGG 7 cut(s) 199, 525, 699, 892, 954, 1431, 1481
Bse3DI GCAATG 3 cut(s) 662, 1599, 1662
BseBI CCWGG 5 cut(s) 283, 864, 1092, 1116, 1568
BseCI ATCGAT 1 cut(s) 1971
BseDI CCNNGG 5 cut(s) 281, 661, 757, 862, 1115
BseGI GGATG 5 cut(s) 382, 712, 753, 1873, 1950
BseLI CCNNNNNNNGG 2 cut(s) 1151, 1696
BseMI GCAATG 3 cut(s) 662, 1599, 1662
BseMII CTCAG 9 cut(s) 236, 290, 312, 419, 647, 1062, 1614, 1762, 1789
BseNI ACTGG 7 cut(s) 199, 525, 699, 892, 954, 1431, 1481
BseRI GAGGAG 2 cut(s) 869, 1989
BseXI GCAGC 3 cut(s) 57, 115, 280
BsgI GTGCAG 2 cut(s) 932, 1249
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 1 cut(s) 36
BshVI ATCGAT 1 cut(s) 1971
BsiEI CGRYCG 1 cut(s) 126
BsiSI CCGG 1 cut(s) 1025
BslFI GGGAC 1 cut(s) 1697
BslI CCNNNNNNNGG 2 cut(s) 1151, 1696
BsmAI GTCTC 4 cut(s) 149, 910, 1067, 1778
BsmBI CGTCTC 1 cut(s) 149
BsmFI GGGAC 1 cut(s) 1697
BsmI GAATGC 1 cut(s) 332
BsnI GGCC 1 cut(s) 36
Bsp1407I TGTACA 3 cut(s) 231, 538, 802
Bsp143I GATC 9 cut(s) 123, 445, 1111, 1141, 1241, 1311, 1477, 1739, 1744
BspACI CCGC 6 cut(s) 201, 330, 735, 1258, 1318, 1954
BspANI GGCC 1 cut(s) 36
BspCNI CTCAG 9 cut(s) 235, 289, 313, 420, 646, 1063, 1613, 1761, 1788
BspDI ATCGAT 1 cut(s) 1971
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 4 cut(s) 447, 861, 1029, 1685
BspPI GGATC 5 cut(s) 440, 453, 1106, 1136, 1472
BspQI GCTCTTC 1 cut(s) 1203
BspTI CTTAAG 1 cut(s) 1349
BsrDI GCAATG 3 cut(s) 662, 1599, 1662
BsrFI RCCGGY 1 cut(s) 1024
BsrGI TGTACA 3 cut(s) 231, 538, 802
BsrI ACTGG 7 cut(s) 199, 525, 699, 892, 954, 1431, 1481
BssAI RCCGGY 1 cut(s) 1024
BssECI CCNNGG 5 cut(s) 281, 661, 757, 862, 1115
BssMI GATC 9 cut(s) 123, 445, 1111, 1141, 1241, 1311, 1477, 1739, 1744
BssNI GRCGYC 2 cut(s) 75, 1489
BssSI CACGAG 1 cut(s) 1827
BssT1I CCWWGG 2 cut(s) 661, 757
Bst2BI CACGAG 1 cut(s) 1827
Bst2UI CCWGG 5 cut(s) 283, 864, 1092, 1116, 1568
Bst4CI ACNGT 5 cut(s) 304, 502, 768, 942, 1588
Bst6I CTCTTC 4 cut(s) 996, 1203, 1566, 1730
BstACI GRCGYC 2 cut(s) 75, 1489
BstAFI CTTAAG 1 cut(s) 1349
BstAUI TGTACA 3 cut(s) 231, 538, 802
BstBAI YACGTR 1 cut(s) 1133
BstC8I GCNNGC 6 cut(s) 527, 1228, 1501, 1546, 1824, 1995
BstF5I GGATG 5 cut(s) 382, 712, 753, 1873, 1950
BstKTI GATC 9 cut(s) 126, 448, 1114, 1144, 1244, 1314, 1480, 1742, 1747
BstMAI GTCTC 4 cut(s) 149, 910, 1067, 1778
BstMBI GATC 9 cut(s) 123, 445, 1111, 1141, 1241, 1311, 1477, 1739, 1744
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 3 cut(s) 42, 103, 1652
BstNI CCWGG 5 cut(s) 283, 864, 1092, 1116, 1568
BstNSI RCATGY 2 cut(s) 610, 1826
BstPAI GACNNNNGTC 1 cut(s) 512
BstSCI CCNGG 5 cut(s) 281, 862, 1090, 1114, 1566
BstV1I GCAGC 3 cut(s) 57, 115, 280
BstX2I RGATCY 4 cut(s) 445, 1111, 1477, 1744
BstXI CCANNNNNNTGG 1 cut(s) 289
BstYI RGATCY 4 cut(s) 445, 1111, 1477, 1744
Bsu15I ATCGAT 1 cut(s) 1971
BsuRI GGCC 1 cut(s) 36
BsuTUI ATCGAT 1 cut(s) 1971
BtgZI GCGATG 1 cut(s) 1229
BtsCI GGATG 5 cut(s) 382, 712, 753, 1873, 1950
BtsIMutI CAGTG 1 cut(s) 1424
Cac8I GCNNGC 6 cut(s) 527, 1228, 1501, 1546, 1824, 1995
CciI TCATGA 1 cut(s) 490
Cfr10I RCCGGY 1 cut(s) 1024
Cfr13I GGNCC 2 cut(s) 859, 1684
ClaI ATCGAT 1 cut(s) 1971
CseI GACGC 2 cut(s) 83, 131
Csp6I GTAC 5 cut(s) 232, 539, 727, 803, 1134
CviAII CATG 9 cut(s) 167, 491, 607, 691, 1763, 1823, 1895, 1988, 2014
CviQI GTAC 5 cut(s) 232, 539, 727, 803, 1134
DpnI GATC 9 cut(s) 125, 447, 1113, 1143, 1243, 1313, 1479, 1741, 1746
DpnII GATC 9 cut(s) 123, 445, 1111, 1141, 1241, 1311, 1477, 1739, 1744
Eam1104I CTCTTC 4 cut(s) 996, 1203, 1566, 1730
EarI CTCTTC 4 cut(s) 996, 1203, 1566, 1730
EciI GGCGGA 1 cut(s) 1247
Eco130I CCWWGG 2 cut(s) 661, 757
Eco47I GGWCC 2 cut(s) 859, 1684
Eco57I CTGAAG 2 cut(s) 1184, 1838
EcoO109I RGGNCCY 1 cut(s) 859
EcoRI GAATTC 1 cut(s) 1556
EcoRII CCWGG 5 cut(s) 281, 862, 1090, 1114, 1566
EcoT14I CCWWGG 2 cut(s) 661, 757
EcoT22I ATGCAT 1 cut(s) 823
ErhI CCWWGG 2 cut(s) 661, 757
Esp3I CGTCTC 1 cut(s) 149
FaeI CATG 9 cut(s) 170, 494, 610, 694, 1766, 1826, 1898, 1991, 2017
FalI AAGNNNNNCTT 2 cut(s) 918, 950
FaqI GGGAC 1 cut(s) 1697
FatI CATG 9 cut(s) 166, 490, 606, 690, 1762, 1822, 1894, 1987, 2013
FauNDI CATATG 1 cut(s) 849
FblI GTMKAC 1 cut(s) 763
Fnu4HI GCNGC 3 cut(s) 46, 104, 294
FokI GGATG 5 cut(s) 389, 719, 740, 1860, 1937
Fsp4HI GCNGC 3 cut(s) 46, 104, 294
FspBI CTAG 8 cut(s) 387, 815, 1332, 1445, 1511, 1647, 1928, 1965
GluI GCNGC 3 cut(s) 46, 104, 294
GsuI CTGGAG 1 cut(s) 1448
HaeIII GGCC 1 cut(s) 36
HapII CCGG 1 cut(s) 1025
HgaI GACGC 2 cut(s) 83, 131
Hin1I GRCGYC 2 cut(s) 75, 1489
Hin1II CATG 9 cut(s) 170, 494, 610, 694, 1766, 1826, 1898, 1991, 2017
HincII GTYRAC 2 cut(s) 764, 828
HindII GTYRAC 2 cut(s) 764, 828
HindIII AAGCTT 1 cut(s) 95
HpaI GTTAAC 1 cut(s) 828
HpaII CCGG 1 cut(s) 1025
HphI GGTGA 2 cut(s) 356, 878
Hpy166II GTNNAC 4 cut(s) 751, 764, 828, 1469
Hpy188III TCNNGA 9 cut(s) 121, 491, 505, 815, 893, 1031, 1676, 1777, 1829
Hpy8I GTNNAC 4 cut(s) 751, 764, 828, 1469
Hpy99I CGWCG 3 cut(s) 59, 77, 1882
HpyAV CCTTC 5 cut(s) 106, 660, 1200, 1410, 1514
HpyCH4III ACNGT 5 cut(s) 304, 502, 768, 942, 1588
HpyCH4IV ACGT 3 cut(s) 1132, 1170, 1489
HpyF10VI GCNNNNNNNGC 3 cut(s) 42, 103, 1652
HpySE526I ACGT 3 cut(s) 1132, 1170, 1489
Hsp92I GRCGYC 2 cut(s) 75, 1489
Hsp92II CATG 9 cut(s) 170, 494, 610, 694, 1766, 1826, 1898, 1991, 2017
KspAI GTTAAC 1 cut(s) 828
Kzo9I GATC 9 cut(s) 123, 445, 1111, 1141, 1241, 1311, 1477, 1739, 1744
LguI GCTCTTC 1 cut(s) 1203
LmnI GCTCC 5 cut(s) 1033, 1429, 1627, 1817, 2002
Lsp1109I GCAGC 3 cut(s) 57, 115, 280
LweI GCATC 4 cut(s) 697, 808, 830, 1206
MaeI CTAG 8 cut(s) 387, 815, 1332, 1445, 1511, 1647, 1928, 1965
MaeII ACGT 3 cut(s) 1132, 1170, 1489
MaeIII GTNAC 9 cut(s) 271, 334, 356, 379, 942, 1081, 1322, 1829, 1983
MalI GATC 9 cut(s) 125, 447, 1113, 1143, 1243, 1313, 1479, 1741, 1746
MboI GATC 9 cut(s) 123, 445, 1111, 1141, 1241, 1311, 1477, 1739, 1744
MboII GAAGA 5 cut(s) 908, 983, 1190, 1583, 1747
MfeI CAATTG 2 cut(s) 1433, 1561
MflI RGATCY 4 cut(s) 445, 1111, 1477, 1744
MlyI GAGTC 1 cut(s) 1616
MmeI TCCRAC 3 cut(s) 78, 594, 1465
Mph1103I ATGCAT 1 cut(s) 823
MroXI GAANNNNTTC 1 cut(s) 1253
MslI CAYNNNNRTG 3 cut(s) 798, 1827, 2007
MspCI CTTAAG 1 cut(s) 1349
MspI CCGG 1 cut(s) 1025
MspR9I CCNGG 5 cut(s) 283, 864, 1092, 1116, 1568
MunI CAATTG 2 cut(s) 1433, 1561
Mva1269I GAATGC 1 cut(s) 332
MvaI CCWGG 5 cut(s) 283, 864, 1092, 1116, 1568
MwoI GCNNNNNNNGC 3 cut(s) 42, 103, 1652
NdeI CATATG 1 cut(s) 849
NdeII GATC 9 cut(s) 123, 445, 1111, 1141, 1241, 1311, 1477, 1739, 1744
NlaIII CATG 9 cut(s) 170, 494, 610, 694, 1766, 1826, 1898, 1991, 2017
NlaIV GGNNCC 4 cut(s) 447, 861, 1029, 1685
NmuCI GTSAC 4 cut(s) 379, 1081, 1322, 1829
NsiI ATGCAT 1 cut(s) 823
NspI RCATGY 2 cut(s) 610, 1826
PaeI GCATGC 1 cut(s) 1826
PagI TCATGA 1 cut(s) 490
PciSI GCTCTTC 1 cut(s) 1203
PcsI WCGNNNNNNNCGW 1 cut(s) 477
PctI GAATGC 1 cut(s) 332
PdmI GAANNNNTTC 1 cut(s) 1253
PfeI GAWTC 9 cut(s) 61, 670, 844, 905, 1095, 1273, 1295, 1870, 1968
PkrI GCNGC 3 cut(s) 47, 105, 295
Ple19I CGATCG 1 cut(s) 126
PleI GAGTC 1 cut(s) 1615
PpsI GAGTC 1 cut(s) 1615
Ppu21I YACGTR 1 cut(s) 1133
PpuMI RGGWCCY 1 cut(s) 859
PshAI GACNNNNGTC 1 cut(s) 512
PshBI ATTAAT 1 cut(s) 1803
Psp1406I AACGTT 1 cut(s) 1170
Psp5II RGGWCCY 1 cut(s) 859
Psp6I CCWGG 5 cut(s) 281, 862, 1090, 1114, 1566
PspGI CCWGG 5 cut(s) 281, 862, 1090, 1114, 1566
PspN4I GGNNCC 4 cut(s) 447, 861, 1029, 1685
PspPI GGNCC 2 cut(s) 859, 1684
PspPPI RGGWCCY 1 cut(s) 859
PsuI RGATCY 4 cut(s) 445, 1111, 1477, 1744
PvuI CGATCG 1 cut(s) 126
RsaI GTAC 5 cut(s) 233, 540, 728, 804, 1135
RsaNI GTAC 5 cut(s) 232, 539, 727, 803, 1134
RseI CAYNNNNRTG 3 cut(s) 798, 1827, 2007
SalI GTCGAC 1 cut(s) 762
SapI GCTCTTC 1 cut(s) 1203
SatI GCNGC 3 cut(s) 46, 104, 294
Sau3AI GATC 9 cut(s) 123, 445, 1111, 1141, 1241, 1311, 1477, 1739, 1744
Sau96I GGNCC 2 cut(s) 859, 1684
ScaI AGTACT 1 cut(s) 728
SchI GAGTC 1 cut(s) 1616
ScrFI CCNGG 5 cut(s) 283, 864, 1092, 1116, 1568
SfaNI GCATC 4 cut(s) 697, 808, 830, 1206
SinI GGWCC 2 cut(s) 859, 1684
SmiMI CAYNNNNRTG 3 cut(s) 798, 1827, 2007
SmlI CTYRAG 4 cut(s) 640, 650, 1289, 1349
SmoI CTYRAG 4 cut(s) 640, 650, 1289, 1349
SpeI ACTAGT 1 cut(s) 1927
SphI GCATGC 1 cut(s) 1826
SsiI CCGC 6 cut(s) 201, 330, 735, 1258, 1318, 1954
SspI AATATT 2 cut(s) 1053, 1066
SspMI CTAG 8 cut(s) 387, 815, 1332, 1445, 1511, 1647, 1928, 1965
StyD4I CCNGG 5 cut(s) 281, 862, 1090, 1114, 1566
StyI CCWWGG 2 cut(s) 661, 757
TaaI ACNGT 5 cut(s) 304, 502, 768, 942, 1588
TaiI ACGT 3 cut(s) 1135, 1173, 1492
TaqI TCGA 7 cut(s) 480, 763, 777, 1383, 1711, 1932, 1971
TatI WGTACW 4 cut(s) 231, 538, 726, 802
TfiI GAWTC 9 cut(s) 61, 670, 844, 905, 1095, 1273, 1295, 1870, 1968
TscAI CASTG 1 cut(s) 1431
TseFI GTSAC 4 cut(s) 379, 1081, 1322, 1829
TseI GCWGC 3 cut(s) 45, 103, 293
Tsp45I GTSAC 4 cut(s) 379, 1081, 1322, 1829
TspDTI ATGAA 8 cut(s) 498, 836, 1378, 1673, 1779, 1789, 1862, 1938
TspGWI ACGGA 3 cut(s) 24, 1866, 1873
TspRI CASTG 1 cut(s) 1431
Vha464I CTTAAG 1 cut(s) 1349
VpaK11BI GGWCC 2 cut(s) 859, 1684
VspI ATTAAT 1 cut(s) 1803
XapI RAATTY 2 cut(s) 714, 1556
XbaI TCTAGA 1 cut(s) 814
XceI RCATGY 2 cut(s) 610, 1826
XmiI GTMKAC 1 cut(s) 763
XmnI GAANNNNTTC 1 cut(s) 1253
XspI CTAG 8 cut(s) 387, 815, 1332, 1445, 1511, 1647, 1928, 1965
ZraI GACGTC 1 cut(s) 1490
ZrmI AGTACT 1 cut(s) 728
Zsp2I ATGCAT 1 cut(s) 823
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.