Rw6G022280

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002639
Physical Location & Seq
Reverse (-)
44063190 .. 44067693
4504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G022280.1

Sequence Viewer

Length: 1701 bp
ATGGCTTCAGTAGGGTCCAATAACTCTAGCGAAAGTCTTAAACGCAACTTCTCTGAAGACAATCACGAATCAGATGCCTTGTTTGCCAAGAAGCAAAAGCTTGCTTTGCACAGAAAGTCTCTCCCAATCGCTACAGTTGAGAACCGGCTCGTCCAGGAGGTTTGGAAACATGATACCCTGATTATTGTTGGCGAAACTGGAAGCGGAAAAACAACGCAATTACCACAGTATTTGTACAATGCTGGATTTTGTGGAAATGGGAGAGTCATTGGGGTAACTCAACCTTGCTGTGTGGCGGCTGTGAATGCCGCGAAGCGGGTTGCTGAGGAATGTGGTGTTAGATTGGGTGAAACGGTTGGTTATTCCATCAGATTTCAGAATGTGACCTCTAGTTCAACAAGGATCAAGTACATGACAGATGGAACTCTCCTGAGGGAAGCGGTATCAGATCCATCTCTCTCTAGGTATTCAGCTATCATTGTGGATGAAGCTCATGAGAGAACTCTTGACACTGATGTATTGCTGGGTGTGCTGAAAAATGTACAAAATGCAAGGTCAGAATCTGTTGAATTGAAATTAATTATCACGTCCGCCAGCTTAGATGCTCGAACTTTCTCCGAATACTTTGGTGGTGCAAGAGCTGTTCACATCCAAGGGAGACAGTTTCCTGTTGCTATATTTTATACTTATCATCTTCCACCATCTTATTTACATGATTCGTTCATTACCATATTTCAGATTCACCTGGAGGAAGAAGCTGGTGGCATACTTGTCTTCTTGACGGGTAAAGACGAAATTGAATTCGTAGAAAGACTTATAGAAGAAAAACTTAAACAGTTACCGGAGAATAGTCAAAAACTAGTAGCGGTACCTTTATTTTCACCTCTTCCGTCGGAACAGCAAATGCGAGCTTTTGCACCAGCCTGTCCTGGGTTTCGTAAGGTAGTTTTGGCAACCAATATTGCTGAGACGTCAGTGACAATACCTGGAATCAAGTATGTTATAGACACTGGGGTTGTAAAAGCACACTTCTATAATCCAAACAAAGGGTTGGAACCTTTGATTGTTGTTCCAGTTTCGAAAGCACAGGCTCTACAAAGGAGTTGGCTTGCAGGGAGAGAAAGACCAGGGAAATGCTTCTGTCTCTACCCAGAGAATGAATTTTGGAAACTTGAGGATTTGACAAAGCCAGATATCACGCGATGCGACCTCTCTAATGCCATTTTGCAACTTAAGGCTTTGGGTGTTCATGATATCACTGGTTTCGACTTCATAGAAAAACCTTCAGGCCAAATTCATGAAAGTGTTGAACAAATGGGCCTCTGTGTTGCATCTTGTGGGGACAACATGCTTCAGTTTCGTAGATGTCTTGCTGCTTCTTTCTTCCTCAGAGTAGCTTTAAAGCAATCTGATGGAGAATACAGGGTTTTAACATCTGATCTGGCAAATGAACTCGTTGTTCAGATTCACCCATCTTCTGTGCTGTCCCAGAAAAAACCAGAGTGCATAGTTTTCAATGAACTAGTTGAAACTAATGACAAAAGGTTTATCTGCAACACGACTAGAATCAACTATCCATGGTTAAGTGAGCTTGCTCCTCCACGACTAAAAAAGGTACTGTATGTTGATTTGACCATTTGGGAATCTTATTGTTGGCCACATAACAGGAATGAAATTATAGGATTTTGCTGTTTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0001817 GO:0001819 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003723 GO:0003724 GO:0003725 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006356 GO:0006396 GO:0006412 GO:0006413 GO:0006518 GO:0006725 GO:0006807 GO:0008026 GO:0008134 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0023056 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032479 GO:0032481 GO:0032991 GO:0033613 GO:0034641 GO:0034645 GO:0042325 GO:0042327 GO:0042623 GO:0043021 GO:0043023 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0043408 GO:0043410 GO:0043484 GO:0043565 GO:0043603 GO:0043604 GO:0044087 GO:0044089 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045937 GO:0045943 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051090 GO:0051091 GO:0051092 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0060255 GO:0061702 GO:0065007 GO:0065009 GO:0070013 GO:0070035 GO:0071704 GO:0072559 GO:0080090 GO:0090304 GO:0097159 GO:0140098 GO:1900225 GO:1900227 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03040 ko03050 ko05169 map03040 map03050 map05169
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

566

Amino Acids

63.32

Weight (kDa)

6.52

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 51 - 203 6.9e-06 DEAD/DEAH box helicase
Helicase_C PF00271 246 - 340 6.3e-07 Helicase conserved C-terminal domain
OB_NTP_bind PF07717 454 - 533 2.8e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 974
Acc65I GGTACC 1 cut(s) 868
AccB1I GGYRCC 1 cut(s) 868
AccII CGCG 2 cut(s) 311, 1201
AciI CCGC 7 cut(s) 204, 296, 309, 316, 440, 591, 866
AclWI GGATC 2 cut(s) 410, 443
AcoI YGGCCR 1 cut(s) 1655
AcsI RAATTY 3 cut(s) 800, 1160, 1293
AcuI CTGAAG 3 cut(s) 75, 1269, 1337
AcyI GRCGYC 1 cut(s) 971
AfaI GTAC 5 cut(s) 236, 410, 543, 870, 1617
AfiI CCNNNNNNNGG 3 cut(s) 315, 930, 1046
AflII CTTAAG 1 cut(s) 1232
AgsI TTSAA 7 cut(s) 396, 569, 574, 800, 1310, 1516, 1529
AhlI ACTAGT 2 cut(s) 859, 1522
AjiI CACGTC 1 cut(s) 588
AjnI CCWGG 5 cut(s) 153, 744, 928, 985, 1126
AluBI AGCT 9 cut(s) 100, 473, 491, 597, 641, 758, 911, 1397, 1591
AluI AGCT 9 cut(s) 100, 473, 491, 597, 641, 758, 911, 1397, 1591
Alw26I GTCTC 4 cut(s) 123, 652, 962, 1148
AlwI GGATC 2 cut(s) 410, 443
AlwNI CAGNNNCTG 1 cut(s) 563
AoxI GGCC 3 cut(s) 1288, 1318, 1655
ApeKI GCWGC 1 cut(s) 1373
ApoI RAATTY 3 cut(s) 800, 1160, 1293
AseI ATTAAT 1 cut(s) 578
Asp700I GAANNNNTTC 1 cut(s) 1136
Asp718I GGTACC 1 cut(s) 868
AspS9I GGNCC 2 cut(s) 15, 1318
AsuHPI GGTGA 4 cut(s) 359, 734, 873, 1460
AsuII TTCGAA 1 cut(s) 1079
AvaII GGWCC 1 cut(s) 15
AxyI CCTNAGG 1 cut(s) 431
BalI TGGCCA 1 cut(s) 1657
BanI GGYRCC 1 cut(s) 868
BbsI GAAGAC 2 cut(s) 63, 766
BbvCI CCTCAGC 1 cut(s) 324
BbvI GCAGC 1 cut(s) 1360
BccI CCATC 6 cut(s) 374, 413, 460, 709, 1406, 1480
BcgI CGANNNNNNTGC 2 cut(s) 56, 90
BciT130I CCWGG 5 cut(s) 155, 746, 930, 987, 1128
BcoDI GTCTC 4 cut(s) 123, 652, 962, 1148
BcuI ACTAGT 2 cut(s) 859, 1522
BfaI CTAG 6 cut(s) 27, 390, 462, 860, 1523, 1563
BfmI CTRYAG 1 cut(s) 132
BfrI CTTAAG 1 cut(s) 1232
BisI GCNGC 3 cut(s) 297, 309, 1374
BlsI GCNGC 3 cut(s) 298, 310, 1375
Bme1390I CCNGG 5 cut(s) 155, 746, 930, 987, 1128
Bme18I GGWCC 1 cut(s) 15
BmgBI CACGTC 1 cut(s) 588
BmgT120I GGNCC 2 cut(s) 15, 1318
BmiI GGNNCC 3 cut(s) 16, 870, 1056
BmrFI CCNGG 5 cut(s) 155, 746, 930, 987, 1128
BmrI ACTGGG 1 cut(s) 1020
BmsI GCATC 4 cut(s) 64, 592, 1193, 1340
BmuI ACTGGG 1 cut(s) 1020
BpiI GAAGAC 2 cut(s) 63, 766
BpmI CTGGAG 1 cut(s) 767
Bpu10I CCTNAGC 1 cut(s) 324
Bpu14I TTCGAA 1 cut(s) 1079
BpuEI CTTGAG 1 cut(s) 1193
BsaHI GRCGYC 1 cut(s) 971
BsaJI CCNNGG 4 cut(s) 652, 929, 1127, 1577
BsaWI WCCGGW 1 cut(s) 841
Bsc4I CCNNNNNNNGG 3 cut(s) 315, 930, 1046
Bse118I RCCGGY 1 cut(s) 144
Bse1I ACTGG 4 cut(s) 202, 1015, 1073, 1264
Bse21I CCTNAGG 1 cut(s) 431
BseBI CCWGG 5 cut(s) 155, 746, 930, 987, 1128
BseDI CCNNGG 4 cut(s) 652, 929, 1127, 1577
BseGI GGATG 2 cut(s) 490, 648
BseLI CCNNNNNNNGG 3 cut(s) 315, 930, 1046
BseMII CTCAG 4 cut(s) 315, 422, 957, 1402
BseNI ACTGG 4 cut(s) 202, 1015, 1073, 1264
BseRI GAGGAG 1 cut(s) 1587
BseXI GCAGC 1 cut(s) 1360
BseYI CCCAGC 1 cut(s) 523
Bsh1236I CGCG 2 cut(s) 311, 1201
BshFI GGCC 3 cut(s) 1290, 1320, 1657
BshNI GGYRCC 1 cut(s) 868
BsiSI CCGG 2 cut(s) 145, 842
BslFI GGGAC 2 cut(s) 1355, 1471
BslI CCNNNNNNNGG 3 cut(s) 315, 930, 1046
BsmAI GTCTC 4 cut(s) 123, 652, 962, 1148
BsmBI CGTCTC 1 cut(s) 962
BsmFI GGGAC 2 cut(s) 1355, 1471
BsmI GAATGC 1 cut(s) 310
BsnI GGCC 3 cut(s) 1290, 1320, 1657
Bsp119I TTCGAA 1 cut(s) 1079
Bsp1407I TGTACA 2 cut(s) 234, 541
Bsp143I GATC 3 cut(s) 402, 448, 1438
Bsp19I CCATGG 1 cut(s) 1577
BspACI CCGC 7 cut(s) 204, 296, 309, 316, 440, 591, 866
BspANI GGCC 3 cut(s) 1290, 1320, 1657
BspCNI CTCAG 4 cut(s) 316, 423, 958, 1401
BspFNI CGCG 2 cut(s) 311, 1201
BspHI TCATGA 3 cut(s) 493, 1249, 1297
BspLI GGNNCC 3 cut(s) 16, 870, 1056
BspPI GGATC 2 cut(s) 410, 443
BspT104I TTCGAA 1 cut(s) 1079
BspT107I GGYRCC 1 cut(s) 868
BspTI CTTAAG 1 cut(s) 1232
BsrFI RCCGGY 1 cut(s) 144
BsrGI TGTACA 2 cut(s) 234, 541
BsrI ACTGG 4 cut(s) 202, 1015, 1073, 1264
BssAI RCCGGY 1 cut(s) 144
BssECI CCNNGG 4 cut(s) 652, 929, 1127, 1577
BssMI GATC 3 cut(s) 402, 448, 1438
BssNI GRCGYC 1 cut(s) 971
BssT1I CCWWGG 2 cut(s) 652, 1577
Bst2UI CCWGG 5 cut(s) 155, 746, 930, 987, 1128
Bst4CI ACNGT 6 cut(s) 136, 228, 355, 663, 837, 1620
Bst6I CTCTTC 1 cut(s) 891
BstACI GRCGYC 1 cut(s) 971
BstAFI CTTAAG 1 cut(s) 1232
BstAUI TGTACA 2 cut(s) 234, 541
BstBI TTCGAA 1 cut(s) 1079
BstC8I GCNNGC 5 cut(s) 102, 595, 909, 1110, 1593
BstDEI CTNAG 5 cut(s) 324, 431, 598, 966, 1388
BstDSI CCRYGG 1 cut(s) 1577
BstF5I GGATG 2 cut(s) 490, 648
BstFNI CGCG 2 cut(s) 311, 1201
BstKTI GATC 3 cut(s) 405, 451, 1441
BstMAI GTCTC 4 cut(s) 123, 652, 962, 1148
BstMBI GATC 3 cut(s) 402, 448, 1438
BstMWI GCNNNNNNNGC 4 cut(s) 83, 106, 305, 529
BstNI CCWGG 5 cut(s) 155, 746, 930, 987, 1128
BstNSI RCATGY 1 cut(s) 1351
BstSCI CCNGG 5 cut(s) 153, 744, 928, 985, 1126
BstSFI CTRYAG 1 cut(s) 132
BstUI CGCG 2 cut(s) 311, 1201
BstV1I GCAGC 1 cut(s) 1360
BstV2I GAAGAC 2 cut(s) 63, 766
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
Bsu36I CCTNAGG 1 cut(s) 431
BsuRI GGCC 3 cut(s) 1290, 1320, 1657
BtgI CCRYGG 1 cut(s) 1577
BtgZI GCGATG 1 cut(s) 1216
BtrI CACGTC 1 cut(s) 588
BtsCI GGATG 2 cut(s) 490, 648
BtsIMutI CAGTG 4 cut(s) 510, 981, 1008, 1257
Cac8I GCNNGC 5 cut(s) 102, 595, 909, 1110, 1593
CaiI CAGNNNCTG 1 cut(s) 563
CciI TCATGA 3 cut(s) 493, 1249, 1297
Cfr10I RCCGGY 1 cut(s) 144
Cfr13I GGNCC 2 cut(s) 15, 1318
Csp6I GTAC 5 cut(s) 235, 409, 542, 869, 1616
CspCI CAANNNNNGTGG 2 cut(s) 213, 248
CviAII CATG 8 cut(s) 170, 412, 494, 713, 1250, 1298, 1348, 1578
CviQI GTAC 5 cut(s) 235, 409, 542, 869, 1616
DdeI CTNAG 5 cut(s) 324, 431, 598, 966, 1388
DpnI GATC 3 cut(s) 404, 450, 1440
DpnII GATC 3 cut(s) 402, 448, 1438
DraI TTTAAA 1 cut(s) 1401
EaeI YGGCCR 1 cut(s) 1655
Eam1104I CTCTTC 1 cut(s) 891
EarI CTCTTC 1 cut(s) 891
EciI GGCGGA 1 cut(s) 580
Eco130I CCWWGG 2 cut(s) 652, 1577
Eco32I GATATC 2 cut(s) 1195, 1255
Eco47I GGWCC 1 cut(s) 15
Eco57I CTGAAG 3 cut(s) 75, 1269, 1337
Eco81I CCTNAGG 1 cut(s) 431
EcoRI GAATTC 1 cut(s) 800
EcoRII CCWGG 5 cut(s) 153, 744, 928, 985, 1126
EcoRV GATATC 2 cut(s) 1195, 1255
EcoT14I CCWWGG 2 cut(s) 652, 1577
ErhI CCWWGG 2 cut(s) 652, 1577
Esp3I CGTCTC 1 cut(s) 962
FaeI CATG 8 cut(s) 173, 415, 497, 716, 1253, 1301, 1351, 1581
FalI AAGNNNNNCTT 2 cut(s) 813, 845
FaqI GGGAC 2 cut(s) 1355, 1471
FatI CATG 8 cut(s) 169, 411, 493, 712, 1249, 1297, 1347, 1577
FauI CCCGC 1 cut(s) 309
Fnu4HI GCNGC 3 cut(s) 297, 309, 1374
FokI GGATG 2 cut(s) 497, 635
Fsp4HI GCNGC 3 cut(s) 297, 309, 1374
FspBI CTAG 6 cut(s) 27, 390, 462, 860, 1523, 1563
GluI GCNGC 3 cut(s) 297, 309, 1374
GsaI CCCAGC 1 cut(s) 527
GsuI CTGGAG 1 cut(s) 767
HaeIII GGCC 3 cut(s) 1290, 1320, 1657
HapII CCGG 2 cut(s) 145, 842
Hin1I GRCGYC 1 cut(s) 971
Hin1II CATG 8 cut(s) 173, 415, 497, 716, 1253, 1301, 1351, 1581
HindIII AAGCTT 1 cut(s) 98
HinfI GANTC 9 cut(s) 68, 264, 560, 716, 739, 990, 1465, 1566, 1643
HpaII CCGG 2 cut(s) 145, 842
HphI GGTGA 4 cut(s) 359, 734, 873, 1460
Hpy166II GTNNAC 1 cut(s) 646
Hpy188III TCNNGA 7 cut(s) 65, 430, 494, 506, 778, 1250, 1298
Hpy8I GTNNAC 1 cut(s) 646
Hpy99I CGWCG 1 cut(s) 895
HpyAV CCTTC 1 cut(s) 1293
HpyCH4III ACNGT 6 cut(s) 136, 228, 355, 663, 837, 1620
HpyCH4IV ACGT 2 cut(s) 587, 971
HpyCH4V TGCA 9 cut(s) 109, 551, 635, 917, 1112, 1228, 1331, 1506, 1554
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 106, 305, 529
HpyF3I CTNAG 5 cut(s) 324, 431, 598, 966, 1388
HpySE526I ACGT 2 cut(s) 587, 971
Hsp92I GRCGYC 1 cut(s) 971
Hsp92II CATG 8 cut(s) 173, 415, 497, 716, 1253, 1301, 1351, 1581
KpnI GGTACC 1 cut(s) 872
Kzo9I GATC 3 cut(s) 402, 448, 1438
LmnI GCTCC 1 cut(s) 1600
Lsp1109I GCAGC 1 cut(s) 1360
LweI GCATC 4 cut(s) 64, 592, 1193, 1340
MaeI CTAG 6 cut(s) 27, 390, 462, 860, 1523, 1563
MaeII ACGT 2 cut(s) 587, 971
MaeIII GTNAC 4 cut(s) 274, 382, 837, 976
MalI GATC 3 cut(s) 404, 450, 1440
MboI GATC 3 cut(s) 402, 448, 1438
MboII GAAGA 8 cut(s) 68, 686, 764, 766, 833, 878, 1375, 1467
MflI RGATCY 1 cut(s) 448
MlsI TGGCCA 1 cut(s) 1657
MluCI AATT 9 cut(s) 218, 569, 575, 579, 795, 800, 1160, 1293, 1674
MluNI TGGCCA 1 cut(s) 1657
MlyI GAGTC 1 cut(s) 273
MmeI TCCRAC 2 cut(s) 873, 1032
Mox20I TGGCCA 1 cut(s) 1657
MroXI GAANNNNTTC 1 cut(s) 1136
MscI TGGCCA 1 cut(s) 1657
MseI TTAA 8 cut(s) 39, 578, 831, 1233, 1400, 1430, 1583, 1699
MslI CAYNNNNRTG 1 cut(s) 1302
Msp20I TGGCCA 1 cut(s) 1657
MspCI CTTAAG 1 cut(s) 1232
MspI CCGG 2 cut(s) 145, 842
MspR9I CCNGG 5 cut(s) 155, 746, 930, 987, 1128
Mva1269I GAATGC 1 cut(s) 310
MvaI CCWGG 5 cut(s) 155, 746, 930, 987, 1128
MvnI CGCG 2 cut(s) 311, 1201
MwoI GCNNNNNNNGC 4 cut(s) 83, 106, 305, 529
NcoI CCATGG 1 cut(s) 1577
NdeII GATC 3 cut(s) 402, 448, 1438
NlaIII CATG 8 cut(s) 173, 415, 497, 716, 1253, 1301, 1351, 1581
NlaIV GGNNCC 3 cut(s) 16, 870, 1056
NmuCI GTSAC 2 cut(s) 382, 976
NspI RCATGY 1 cut(s) 1351
NspV TTCGAA 1 cut(s) 1079
PagI TCATGA 3 cut(s) 493, 1249, 1297
PctI GAATGC 1 cut(s) 310
PdmI GAANNNNTTC 1 cut(s) 1136
PfeI GAWTC 8 cut(s) 68, 560, 716, 739, 990, 1465, 1566, 1643
PfoI TCCNGGA 1 cut(s) 153
PkrI GCNGC 3 cut(s) 298, 310, 1375
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PshBI ATTAAT 1 cut(s) 578
Psp6I CCWGG 5 cut(s) 153, 744, 928, 985, 1126
PspFI CCCAGC 1 cut(s) 523
PspGI CCWGG 5 cut(s) 153, 744, 928, 985, 1126
PspN4I GGNNCC 3 cut(s) 16, 870, 1056
PspPI GGNCC 2 cut(s) 15, 1318
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 1 cut(s) 448
RsaI GTAC 5 cut(s) 236, 410, 543, 870, 1617
RsaNI GTAC 5 cut(s) 235, 409, 542, 869, 1616
RseI CAYNNNNRTG 1 cut(s) 1302
SaqAI TTAA 8 cut(s) 39, 578, 831, 1233, 1400, 1430, 1583, 1699
SatI GCNGC 3 cut(s) 297, 309, 1374
Sau3AI GATC 3 cut(s) 402, 448, 1438
Sau96I GGNCC 2 cut(s) 15, 1318
SchI GAGTC 1 cut(s) 273
ScrFI CCNGG 5 cut(s) 155, 746, 930, 987, 1128
SfaNI GCATC 4 cut(s) 64, 592, 1193, 1340
SfcI CTRYAG 1 cut(s) 132
SfuI TTCGAA 1 cut(s) 1079
SinI GGWCC 1 cut(s) 15
SmiMI CAYNNNNRTG 1 cut(s) 1302
SmlI CTYRAG 2 cut(s) 1172, 1232
SmoI CTYRAG 2 cut(s) 1172, 1232
SpeI ACTAGT 2 cut(s) 859, 1522
Sse9I AATT 9 cut(s) 218, 569, 575, 579, 795, 800, 1160, 1293, 1674
SsiI CCGC 7 cut(s) 204, 296, 309, 316, 440, 591, 866
SspI AATATT 1 cut(s) 961
SspMI CTAG 6 cut(s) 27, 390, 462, 860, 1523, 1563
StyD4I CCNGG 5 cut(s) 153, 744, 928, 985, 1126
StyI CCWWGG 2 cut(s) 652, 1577
TaaI ACNGT 6 cut(s) 136, 228, 355, 663, 837, 1620
TaiI ACGT 2 cut(s) 590, 974
TaqI TCGA 3 cut(s) 607, 1079, 1266
TasI AATT 9 cut(s) 218, 569, 575, 579, 795, 800, 1160, 1293, 1674
TatI WGTACW 3 cut(s) 234, 408, 541
TauI GCSGC 2 cut(s) 299, 311
TfiI GAWTC 8 cut(s) 68, 560, 716, 739, 990, 1465, 1566, 1643
Tru1I TTAA 8 cut(s) 39, 578, 831, 1233, 1400, 1430, 1583, 1699
Tru9I TTAA 8 cut(s) 39, 578, 831, 1233, 1400, 1430, 1583, 1699
TscAI CASTG 4 cut(s) 517, 981, 1015, 1264
TseFI GTSAC 2 cut(s) 382, 976
TseI GCWGC 1 cut(s) 1373
Tsp45I GTSAC 2 cut(s) 382, 976
TspGWI ACGGA 1 cut(s) 879
TspRI CASTG 4 cut(s) 517, 981, 1015, 1264
Vha464I CTTAAG 1 cut(s) 1232
VpaK11BI GGWCC 1 cut(s) 15
VspI ATTAAT 1 cut(s) 578
XapI RAATTY 3 cut(s) 800, 1160, 1293
XceI RCATGY 1 cut(s) 1351
XmnI GAANNNNTTC 1 cut(s) 1136
XspI CTAG 6 cut(s) 27, 390, 462, 860, 1523, 1563
ZraI GACGTC 1 cut(s) 972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.