Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0003027

Physical Location & Seq

Genomic Coordinates Forward (+)

32000300 .. 32009172

Gene Structure 8873 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g33990.t14

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3564 bp

ATGAACCTGGACACCGATTTCACATCCGCATTGCTCCACGATTTGAAGCTCGAAGATCCTTTGCTTCCTCCAACCACATGGGAATCCATCCCTTCCGAAAGCGGCCATTTCCATCTTCCGTCATCCAATCCTCTCTCCCTCTACCATGCCTCCTCCGTCTCCGAGGCAAGTTTGGTGCGATTGGCGATGAATGCATTGCAAGGACTTGAATCGTCGCTGATTAGTGTCCAGAAGCTTTCGGCTGCGTTTCGGTCCGACGCGGCGGATAGGACATTTCATCAGGTCCCGAGCCTGTGGAGTAGGTCTTCGAGCACTCAGGCTCTGGGGAATATTCTCCACTCCATAGGCTGCTCAGGTTTGCTGGTCTTTCTTCTTGGAAAATTTGTGGATCACTTTACCAATTTAGATGTCGAAAGTGGAAGTAAAGAAAGAGAGTCTGCTCCGTATAGTCTTGTTAATCAGGCTTTTGTGGTTGCGGTGGGGAAGGTTGTGGAGGGTTACATGTGTGCTTTGGATACATTGTATGCTTCTGTGGGGTTGAGGCGGGGCTCGCCACCTTCTGCGGTGGGGTGTCTCAACAGTGTGGTCCATTCGGAAGTTAGTTTGTTGGAGGTGTATTTGCACACTAAGGAATTGAGGACTCAGATTGAATCCCTTGCAAACATATGCAACTTGTATCGATTTTCTGATTGCTTCGCAGCATCTTGTTTTGATGAGTTGCTTGCGGAAGCCAAATCTGAGTTTTGTAATTTCTACAGAGGGGGTGACCTACTCACATATCTGTATACTCAATTGCAGGTTGCTGATCCTGCTCATCGTCCTGTACTAAAATTTCTTTTTCTTCGTGCATGTGAACCATACTTCGGATTTATAAGATCATGGATATTCAAAGCTGAGATTAGCGATCCTTATGAGGAATTTTTGGTTGAATATGTTCAGTCTCATGATCCATATGTTGAAGCCAGTATCTCTACTGACTTCCCATTGGCAACTATCAGGGAACGAGATGGAGTTCCTGTTCCTTGTTTTCTGAAGGACATCTTGATTCCACTTTTGAGAGCAGGTCAGCAGCTGCAAGTTCTAGCAAAATTGCTTCAATTGTGTACTTTTGTTGCCCCTACGAACCATACTTATGAGACTTTTCTTCCTTGCTGGAGTGGGTTTTCAAGCAATTGTCCATCGTATGCATCTCCATTGACATTCAGCAAAGGAAATATAGAGGCCATGGTACTTTCACGGGAATGTTATTACAAAAAGATGCAAGACAGACTCCAGATTCTTTTGACGGAATTAGAGTTCAAATTTCAACAGGTAGTTCCACAAGACGCGCTTGTTCTCTTGGATCACAGTGGAAGGAGTTCAGCCATCCCAGTTTTAGTCCCATCGGATAAAAGTTATATTGAAAAGGAGTCACAAGAGGTGGCTGATGATTTGGATTCGGACGAGTTAAGTACAAGAGACTACACTGCAGATGAATACGAGTCATCTGAATGTTCAAGCTCAACTATGTCTGAAGAGCAAAACGTGCTCAAGCGGACACCTGAGTTTCCTAGTCACATCATAGTGGAAGAACAAAAGTATTTCTCTGCTTTAAGCTTCTCAATAGGTATTCCGGTTGATACTGTGCAAAAGCCCCATGGATGTGCAGATTCATGTCTTATAGACAAGATTAGTAGTTGTGAAAGAGAAGATGCCTTTGGGCATTCTCATCATAAAGGCATACTTATGAGTCAGATGTTAGACGTTCAATTTTCAGATTGTCTCTCTGATAAGGATTGGCCTGATTATTTTGAGAAACAGTCTGTCATTGATATAGAGTACAAAGAAGGTCTGAAGTCAAGTCCAACAGACTTTGCCTCAAAGGTGAATGAAAGAATAACGGGAGCCTTAAAGGAAGGTTCATCATATTTTAGAAAAAGAATCATGGATAATAATGCATCAATAGAAGAAGCCTTTGGCAAGATTGAATCTCAAAAGGCCAGTAATACTTCTGATACATTTGCATTGCGACAGTGGAAGGTTAATTACCATAACAATTTTCTCAGCATGAACCCAATGTTGACAAAAAATAATTTCCTTCACCTAACTTCCAGGCCTGAAGAGAGATGCAAGACAGACCATGGGGATTCTTTGGCTTACTTTGATTTCTCTCACATAAAAGATCCTTGTAAGGTGTTTCCGATGAAGGTTCCTGTAGGGCTTATGGACTATAGGGCCTCTAATACCAATGGCAGGAGTGATCACCATGTAAAAGAATGTTCTTCCGGAGATGATGTTCTGATTGGAAGAACAATAGTATCTGACTTTCTTCCATCTTCAGATTTGAGGGGCCACAGTGAAGGAGATGCTACTTCAACAAATGTTTCTGGTGGTAGTTGTTGGGAGAGTTTGCTTGGTAGATTTGGTGACACTGTCGTAAATAGACTTGAAGATCACAGAGAAAGCTTGGCGGCCAGTTTTGACATGCCACTTGATTTTATTATTGACAAATGCCTTCTGCAGGAAATCATGCTCCAATATAAGTATGTCAGCAAGTTAACTATTAACTTGCTTGAAGAAGGATTTGATTTGCAAGAACATTTGCTGGCTCTGCGTCGGTACCACTTCATGGAATTAGCAGACTGGGCAGATTTGTTTATTATGTCTCTTTGGCATCATAAATGGAGTATTACACAGGCAGATCATAGACTTTCTGAAATTCAAAGTTTTCTTGAGTCATCGGTTCAGAGGTCATCATGTGAACGTGATCGTAATAAGGACAGATTATTTATGTACTTGAAAGGACATGATGCAGTGCCGCTTTCAGCATCTGCTATAGGAGTCCATTCCTTTAACTTCCTTGGTTTGGGATATCGAGTAGACTGGCCCATCAGCATTGTTTTGACTCCTGGTGCATTGAAAAAATATGCCGAGATATTCAGTTTCTTGATACAAGTGAAACTTGCTCTGTTTTCGTTGACTGATGTATGGCGCCAATTGAAGATGCAGGATCTGATCCACTTGATTTATCGGAATAACCACTCTGAAGAACATGAGAAGGAAGTGAGCCATTTTAATGCAATCGTGAAAATGAGGCATCAGGTCAATCACTTTGTTTCTACATTGCAGCAGTATGTGGAGTCGCAGCTTTCACATGTATCATGGTGCAGGTTTCTATACTCACTTAAGCATAAGGTCAAAGATATGATGGATCTTCAGTCTGTGCACATGGCATATCTCTCAGATTCACTTCACATATGTTTCCTCTCTGATGAAACAAAACCTATAGCTTGCATCATTGAGAGCATTCTGCAGTGTGCTCTAGACTTCAGGTCTTGTCTTACTGGGGGAGTATGGAATGCTGGAACAGGTCAAGGGAATTTAGTTGCAACACTCTCTGGAATCAATATATCCCAGGTTATTGCCATAAAGCGAACCTTTGACAAAAATATGAAAGAATTGCACTCCTGTTACCTCAAATCACCTAAACATGGTGAGTTTGGACTCTCTCACTTCTGGGAATACCTGAATTACAACAGGTATTACTCAGATGCTGCTTCCGTGGTCCACAGCTCAGTAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000226 GO:0000278 GO:0000280 GO:0000923 GO:0000930 GO:0000931 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005813 GO:0005815 GO:0005816 GO:0005822 GO:0005825 GO:0005829 GO:0005856 GO:0006810 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007020 GO:0007049 GO:0007051 GO:0007052 GO:0007097 GO:0007098 GO:0007163 GO:0008017 GO:0008092 GO:0008150 GO:0008274 GO:0008275 GO:0008360 GO:0009987 GO:0015630 GO:0015631 GO:0016043 GO:0022402 GO:0022414 GO:0022603 GO:0022604 GO:0022607 GO:0031021 GO:0031023 GO:0031109 GO:0031122 GO:0032153 GO:0032155 GO:0032991 GO:0034453 GO:0034622 GO:0034631 GO:0043015 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044450 GO:0044464 GO:0044732 GO:0046785 GO:0046907 GO:0048285 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051179 GO:0051225 GO:0051234 GO:0051258 GO:0051298 GO:0051321 GO:0051415 GO:0051418 GO:0051640 GO:0051641 GO:0051647 GO:0051649 GO:0051656 GO:0061496 GO:0061497 GO:0065003 GO:0065007 GO:0065008 GO:0070925 GO:0071840 GO:0071957 GO:0071963 GO:0072393 GO:0090307 GO:0097435 GO:0098863 GO:0099098 GO:0099111 GO:0140014 GO:1902850 GO:1903047 GO:1990734

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Spc97_Spc98

Protein Analysis

1188

Amino Acids

134.23

Weight (kDa)

5.58

Isoelectric Point (pI)

48.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GCP_N_terminal	PF17681	63 - 368	1.3e-24	Gamma tubulin complex component N-terminal
GCP_C_terminal	PF04130	857 - 997	3.4e-30	Gamma tubulin complex component C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003027)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43610 AT3G43610 AT3G43610
fragaria_vesca	FvH4_3g27030 FvH4_3g27031 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990
malus_domestica	MD03G1162300.v1.1 MD03G1162400.v1.1 MD11G1178600.v1.1
prunus_persica	Prupe.6G151400_v2.0.a1 Prupe.6G151400_v2.0.a1 Prupe.6G151400_v2.0.a1
pyrus_communis	pycom11g15400
rosa_chinensis	RchiOBHm_Chr5g0049541
rosa_laevigata	RLG00000034625
rosa_multiflora	Rmu_co8383485.1_g000001 Rmu_sc0013032.1_g000004
rosa_roxburghii	Rroxscaffold_1G00032160
rosa_rugosa	Rorug05G0248900 Rorug05G0249000
rosa_samantha	Rh5AG325500 Rh5BG336700 Rh5CG361500 Rh5DG348800
rosa_wichuraiana	Rw5G030750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	872
Acc36I	ACCTGC	3 cut(s)	787, 1052, 3138
Acc65I	GGTACC	1 cut(s)	2598
AccB1I	GGYRCC	2 cut(s)	2598, 2970
AccB7I	CCANNNNNTGG	1 cut(s)	2608
AccI	GTMKAC	2 cut(s)	785, 2859
AccII	CGCG	2 cut(s)	260, 1328
AccIII	TCCGGA	1 cut(s)	2264
AcoI	YGGCCR	2 cut(s)	103, 2451
AcsI	RAATTY	6 cut(s)	380, 830, 917, 1301, 2697, 3358
AcuI	CTGAAG	8 cut(s)	1052, 1533, 1853, 2118, 2301, 3045, 3179, 3292
AcyI	GRCGYC	1 cut(s)	2971
AfaI	GTAC	7 cut(s)	825, 1105, 1230, 1453, 1820, 2600, 2774
AfiI	CCNNNNNNNGG	6 cut(s)	863, 2232, 2500, 2608, 2845, 3470
AflII	CTTAAG	1 cut(s)	3164
AflIII	ACRYGT	2 cut(s)	501, 3133
AhdI	GACNNNNNGTC	1 cut(s)	3310
AjnI	CCWGG	4 cut(s)	6, 2090, 2887, 3393
AjuI	GAANNNNNNNTTGG	4 cut(s)	1680, 1712, 1938, 1970
AloI	GAACNNNNNNTCC	6 cut(s)	1002, 1034, 1280, 1312, 2259, 2291
Alw21I	GWGCWC	4 cut(s)	314, 1530, 3207, 3301
Alw26I	GTCTC	7 cut(s)	163, 578, 945, 1130, 1452, 1766, 2649
Alw44I	GTGCAC	1 cut(s)	3203
AlwNI	CAGNNNCTG	5 cut(s)	322, 1072, 2810, 2992, 3533
Ama87I	CYCGRG	1 cut(s)	286
Aor13HI	TCCGGA	1 cut(s)	2264
AoxI	GGCC	9 cut(s)	103, 1221, 1778, 1977, 2093, 2214, 2329, 2451, 2864
ApaLI	GTGCAC	1 cut(s)	3203
ApeKI	GCWGC	8 cut(s)	242, 348, 698, 1069, 1072, 3106, 3124, 3533
ApoI	RAATTY	6 cut(s)	380, 830, 917, 1301, 2697, 3358
Asp700I	GAANNNNTTC	2 cut(s)	933, 1357
Asp718I	GGTACC	1 cut(s)	2598
AspLEI	GCGC	2 cut(s)	1330, 2973
AspS9I	GGNCC	7 cut(s)	252, 283, 586, 2214, 2329, 2865, 3544
AsuHPI	GGTGA	7 cut(s)	776, 1876, 2072, 2234, 2417, 3453, 3485
AvaI	CYCGRG	1 cut(s)	286
AvaII	GGWCC	4 cut(s)	252, 283, 586, 3544
BaeGI	GKGCMC	1 cut(s)	3207
BaeI	ACNNNNGTAYC	2 cut(s)	2280, 2313
BanI	GGYRCC	2 cut(s)	2598, 2970
BanII	GRGCYC	1 cut(s)	551
BarI	GAAGNNNNNNTAC	2 cut(s)	2009, 2041
BbsI	GAAGAC	1 cut(s)	297
Bbv12I	GWGCWC	4 cut(s)	314, 1530, 3207, 3301
BbvI	GCAGC	8 cut(s)	229, 335, 710, 1059, 1081, 3118, 3136, 3520
BccI	CCATC	9 cut(s)	95, 120, 1001, 1186, 1373, 1390, 2320, 2876, 3181
BciT130I	CCWGG	4 cut(s)	8, 2092, 2889, 3395
BciVI	GTATCC	1 cut(s)	508
BclI	TGATCA	1 cut(s)	2239
BcoDI	GTCTC	7 cut(s)	163, 578, 945, 1130, 1452, 1766, 2649
BfaI	CTAG	3 cut(s)	1082, 1551, 3302
BfmI	CTRYAG	8 cut(s)	754, 1467, 2193, 2209, 2498, 2814, 3264, 3290
BfoI	RGCGCY	1 cut(s)	2974
BfrI	CTTAAG	1 cut(s)	3164
BfuAI	ACCTGC	3 cut(s)	787, 1052, 3138
BfuI	GTATCC	1 cut(s)	508
Bme1390I	CCNGG	4 cut(s)	8, 2092, 2889, 3395
Bme18I	GGWCC	4 cut(s)	252, 283, 586, 3544
BmeRI	GACNNNNNGTC	1 cut(s)	3310
BmeT110I	CYCGRG	1 cut(s)	286
BmgT120I	GGNCC	7 cut(s)	252, 283, 586, 2214, 2329, 2865, 3544
BmiI	GGNNCC	6 cut(s)	285, 1885, 2190, 2330, 2600, 2972
BmrFI	CCNGG	4 cut(s)	8, 2092, 2889, 3395
BmrI	ACTGGG	3 cut(s)	1364, 2632, 3333
BmuI	ACTGGG	3 cut(s)	1364, 2632, 3333
BpiI	GAAGAC	1 cut(s)	297
BpmI	CTGGAG	2 cut(s)	1174, 1256
Bpu10I	CCTNAGC	1 cut(s)	352
BpuEI	CTTGAG	2 cut(s)	1514, 2732
Bsa29I	ATCGAT	1 cut(s)	679
BsaHI	GRCGYC	1 cut(s)	2971
BsaJI	CCNNGG	7 cut(s)	162, 1224, 1636, 2119, 2839, 3393, 3540
BsaWI	WCCGGW	2 cut(s)	1612, 2264
BsaXI	ACNNNNNCTCC	6 cut(s)	134, 164, 1002, 1032, 2259, 2289
Bsc4I	CCNNNNNNNGG	6 cut(s)	863, 2232, 2500, 2608, 2845, 3470
Bse1I	ACTGG	7 cut(s)	963, 1370, 1980, 2454, 2627, 2867, 3328
Bse3DI	GCAATG	4 cut(s)	29, 194, 2003, 3101
BseAI	TCCGGA	1 cut(s)	2264
BseBI	CCWGG	4 cut(s)	8, 2092, 2889, 3395
BseCI	ATCGAT	1 cut(s)	679
BseDI	CCNNGG	7 cut(s)	162, 1224, 1636, 2119, 2839, 3393, 3540
BseGI	GGATG	5 cut(s)	23, 87, 122, 1365, 1646
BseLI	CCNNNNNNNGG	6 cut(s)	863, 2232, 2500, 2608, 2845, 3470
BseMI	GCAATG	4 cut(s)	29, 194, 2003, 3101
BseMII	CTCAG	9 cut(s)	329, 366, 656, 729, 885, 1533, 2056, 3234, 3540
BseNI	ACTGG	7 cut(s)	963, 1370, 1980, 2454, 2627, 2867, 3328
BseRI	GAGGAG	1 cut(s)	142
BseSI	GKGCMC	1 cut(s)	3207
BseXI	GCAGC	8 cut(s)	229, 335, 710, 1059, 1081, 3118, 3136, 3520
BsgI	GTGCAG	2 cut(s)	1665, 3166
Bsh1236I	CGCG	2 cut(s)	260, 1328
BshFI	GGCC	9 cut(s)	105, 1223, 1780, 1979, 2095, 2216, 2331, 2453, 2866
BshNI	GGYRCC	2 cut(s)	2598, 2970
BshVI	ATCGAT	1 cut(s)	679
BsiHKAI	GWGCWC	4 cut(s)	314, 1530, 3207, 3301
BsiHKCI	CYCGRG	1 cut(s)	286
BsiSI	CCGG	2 cut(s)	1613, 2265
BslFI	GGGAC	2 cut(s)	269, 1364
BslI	CCNNNNNNNGG	6 cut(s)	863, 2232, 2500, 2608, 2845, 3470
BsmAI	GTCTC	7 cut(s)	163, 578, 945, 1130, 1452, 1766, 2649
BsmBI	CGTCTC	1 cut(s)	163
BsmFI	GGGAC	2 cut(s)	269, 1364
BsmI	GAATGC	4 cut(s)	196, 1702, 3285, 3343
BsnI	GGCC	9 cut(s)	105, 1223, 1780, 1979, 2095, 2216, 2331, 2453, 2866
BsoBI	CYCGRG	1 cut(s)	286
Bsp1286I	GDGCHC	5 cut(s)	314, 551, 1530, 3207, 3301
Bsp13I	TCCGGA	1 cut(s)	2264
Bsp19I	CCATGG	3 cut(s)	1224, 1636, 2119
BspANI	GGCC	9 cut(s)	105, 1223, 1780, 1979, 2095, 2216, 2331, 2453, 2866
BspCNI	CTCAG	9 cut(s)	328, 365, 655, 730, 886, 1534, 2055, 3233, 3539
BspDI	ATCGAT	1 cut(s)	679
BspEI	TCCGGA	1 cut(s)	2264
BspFNI	CGCG	2 cut(s)	260, 1328
BspHI	TCATGA	1 cut(s)	943
BspLI	GGNNCC	6 cut(s)	285, 1885, 2190, 2330, 2600, 2972
BspMAI	CTGCAG	3 cut(s)	1471, 2502, 3294
BspMI	ACCTGC	3 cut(s)	787, 1052, 3138
BspQI	GCTCTTC	1 cut(s)	1509
BspT107I	GGYRCC	2 cut(s)	2598, 2970
BspTI	CTTAAG	1 cut(s)	3164
BsrDI	GCAATG	4 cut(s)	29, 194, 2003, 3101
BsrI	ACTGG	7 cut(s)	963, 1370, 1980, 2454, 2627, 2867, 3328
BssECI	CCNNGG	7 cut(s)	162, 1224, 1636, 2119, 2839, 3393, 3540
BssNAI	GTATAC	1 cut(s)	786
BssNI	GRCGYC	1 cut(s)	2971
BssT1I	CCWWGG	4 cut(s)	1224, 1636, 2119, 2839
Bst1107I	GTATAC	1 cut(s)	786
Bst2UI	CCWGG	4 cut(s)	8, 2092, 2889, 3395
Bst4CI	ACNGT	7 cut(s)	581, 1349, 1624, 1800, 2013, 2336, 2413
Bst6I	CTCTTC	2 cut(s)	1509, 2094
BstACI	GRCGYC	1 cut(s)	2971
BstAFI	CTTAAG	1 cut(s)	3164
BstAPI	GCANNNNNTGC	1 cut(s)	1525
BstC8I	GCNNGC	4 cut(s)	551, 723, 2586, 3271
BstDSI	CCRYGG	4 cut(s)	1224, 1636, 2119, 3540
BstEII	GGTNACC	1 cut(s)	764
BstENI	CCTNNNNNAGG	1 cut(s)	2498
BstF5I	GGATG	5 cut(s)	23, 87, 122, 1365, 1646
BstFNI	CGCG	2 cut(s)	260, 1328
BstH2I	RGCGCY	1 cut(s)	2974
BstHHI	GCGC	2 cut(s)	1330, 2973
BstMAI	GTCTC	7 cut(s)	163, 578, 945, 1130, 1452, 1766, 2649
BstMWI	GCNNNNNNNGC	6 cut(s)	191, 550, 809, 1525, 2590, 2624
BstNI	CCWGG	4 cut(s)	8, 2092, 2889, 3395
BstNSI	RCATGY	4 cut(s)	505, 852, 2467, 3137
BstPI	GGTNACC	1 cut(s)	764
BstSCI	CCNGG	4 cut(s)	6, 2090, 2887, 3393
BstSFI	CTRYAG	8 cut(s)	754, 1467, 2193, 2209, 2498, 2814, 3264, 3290
BstSLI	GKGCMC	1 cut(s)	3207
BstUI	CGCG	2 cut(s)	260, 1328
BstV1I	GCAGC	8 cut(s)	229, 335, 710, 1059, 1081, 3118, 3136, 3520
BstV2I	GAAGAC	1 cut(s)	297
BstX2I	RGATCY	4 cut(s)	55, 2161, 2989, 3190
BstXI	CCANNNNNNTGG	1 cut(s)	78
BstYI	RGATCY	4 cut(s)	55, 2161, 2989, 3190
BstZ17I	GTATAC	1 cut(s)	786
Bsu15I	ATCGAT	1 cut(s)	679
BsuI	GTATCC	1 cut(s)	508
BsuRI	GGCC	9 cut(s)	105, 1223, 1780, 1979, 2095, 2216, 2331, 2453, 2866
BsuTUI	ATCGAT	1 cut(s)	679
BtgI	CCRYGG	4 cut(s)	1224, 1636, 2119, 3540
BtgZI	GCGATG	1 cut(s)	200
BtsCI	GGATG	5 cut(s)	23, 87, 122, 1365, 1646
BtsI	GCAGTG	3 cut(s)	1464, 2799, 3299
BtsIMutI	CAGTG	8 cut(s)	586, 1354, 1464, 2018, 2341, 2409, 2799, 3299
BveI	ACCTGC	3 cut(s)	787, 1052, 3138
Cac8I	GCNNGC	4 cut(s)	551, 723, 2586, 3271
CaiI	CAGNNNCTG	5 cut(s)	322, 1072, 2810, 2992, 3533
CciI	TCATGA	1 cut(s)	943
CfoI	GCGC	2 cut(s)	1330, 2973
Cfr13I	GGNCC	7 cut(s)	252, 283, 586, 2214, 2329, 2865, 3544
ClaI	ATCGAT	1 cut(s)	679
CpoI	CGGWCCG	1 cut(s)	252
CseI	GACGC	3 cut(s)	266, 1334, 2582
Csp6I	GTAC	7 cut(s)	824, 1104, 1229, 1452, 1819, 2599, 2773
CspI	CGGWCCG	1 cut(s)	252
CviQI	GTAC	7 cut(s)	824, 1104, 1229, 1452, 1819, 2599, 2773
DinI	GGCGCC	1 cut(s)	2972
DriI	GACNNNNNGTC	1 cut(s)	3310
EaeI	YGGCCR	2 cut(s)	103, 2451
Eam1104I	CTCTTC	2 cut(s)	1509, 2094
Eam1105I	GACNNNNNGTC	1 cut(s)	3310
EarI	CTCTTC	2 cut(s)	1509, 2094
EciI	GGCGGA	1 cut(s)	278
Eco130I	CCWWGG	4 cut(s)	1224, 1636, 2119, 2839
Eco147I	AGGCCT	1 cut(s)	2095
Eco24I	GRGCYC	1 cut(s)	551
Eco32I	GATATC	1 cut(s)	2852
Eco47I	GGWCC	4 cut(s)	252, 283, 586, 3544
Eco57I	CTGAAG	8 cut(s)	1052, 1533, 1853, 2118, 2301, 3045, 3179, 3292
Eco88I	CYCGRG	1 cut(s)	286
Eco91I	GGTNACC	1 cut(s)	764
EcoNI	CCTNNNNNAGG	1 cut(s)	2498
EcoO109I	RGGNCCY	2 cut(s)	283, 2214
EcoO65I	GGTNACC	1 cut(s)	764
EcoRII	CCWGG	4 cut(s)	6, 2090, 2887, 3393
EcoRV	GATATC	1 cut(s)	2852
EcoT14I	CCWWGG	4 cut(s)	1224, 1636, 2119, 2839
EcoT22I	ATGCAT	3 cut(s)	196, 1189, 1939
EcoT38I	GRGCYC	1 cut(s)	551
EgeI	GGCGCC	1 cut(s)	2972
EheI	GGCGCC	1 cut(s)	2972
ErhI	CCWWGG	4 cut(s)	1224, 1636, 2119, 2839
Esp3I	CGTCTC	1 cut(s)	163
FaqI	GGGAC	2 cut(s)	269, 1364
FauI	CCCGC	1 cut(s)	537
FauNDI	CATATG	3 cut(s)	665, 952, 3236
FbaI	TGATCA	1 cut(s)	2239
FblI	GTMKAC	2 cut(s)	785, 2859
FokI	GGATG	5 cut(s)	10, 74, 109, 1352, 1653
FriOI	GRGCYC	1 cut(s)	551
FspBI	CTAG	3 cut(s)	1082, 1551, 3302
GlaI	GCGC	2 cut(s)	1329, 2972
GsuI	CTGGAG	2 cut(s)	1174, 1256
HaeII	RGCGCY	1 cut(s)	2974
HaeIII	GGCC	9 cut(s)	105, 1223, 1780, 1979, 2095, 2216, 2331, 2453, 2866
HapII	CCGG	2 cut(s)	1613, 2265
HgaI	GACGC	3 cut(s)	266, 1334, 2582
HhaI	GCGC	2 cut(s)	1330, 2973
Hin1I	GRCGYC	1 cut(s)	2971
Hin6I	GCGC	2 cut(s)	1328, 2971
HinP1I	GCGC	2 cut(s)	1328, 2971
HincII	GTYRAC	3 cut(s)	2061, 2538, 2958
HindII	GTYRAC	3 cut(s)	2061, 2538, 2958
HindIII	AAGCTT	3 cut(s)	233, 1594, 2443
HpaI	GTTAAC	1 cut(s)	2538
HpaII	CCGG	2 cut(s)	1613, 2265
HphI	GGTGA	7 cut(s)	776, 1876, 2072, 2234, 2417, 3453, 3485
Hpy99I	CGWCG	3 cut(s)	217, 260, 2598
HpyCH4III	ACNGT	7 cut(s)	581, 1349, 1624, 1800, 2013, 2336, 2413
HpyCH4IV	ACGT	3 cut(s)	1524, 1743, 2743
HpyF10VI	GCNNNNNNNGC	6 cut(s)	191, 550, 809, 1525, 2590, 2624
HpySE526I	ACGT	3 cut(s)	1524, 1743, 2743
Hsp92I	GRCGYC	1 cut(s)	2971
HspAI	GCGC	2 cut(s)	1328, 2971
KasI	GGCGCC	1 cut(s)	2970
Kpn2I	TCCGGA	1 cut(s)	2264
KpnI	GGTACC	1 cut(s)	2602
Ksp22I	TGATCA	1 cut(s)	2239
KspAI	GTTAAC	1 cut(s)	2538
LguI	GCTCTTC	1 cut(s)	1509
LmnI	GCTCC	4 cut(s)	39, 445, 1883, 2517
Lsp1109I	GCAGC	8 cut(s)	229, 335, 710, 1059, 1081, 3118, 3136, 3520
MaeI	CTAG	3 cut(s)	1082, 1551, 3302
MaeII	ACGT	3 cut(s)	1524, 1743, 2743
MaeIII	GTNAC	7 cut(s)	497, 764, 1410, 1553, 2405, 3449, 3556
MfeI	CAATTG	4 cut(s)	791, 1097, 1171, 2975
MflI	RGATCY	4 cut(s)	55, 2161, 2989, 3190
MhlI	GDGCHC	5 cut(s)	314, 551, 1530, 3207, 3301
Mly113I	GGCGCC	1 cut(s)	2971
MmeI	TCCRAC	4 cut(s)	95, 279, 588, 1868
Mph1103I	ATGCAT	3 cut(s)	196, 1189, 1939
MroI	TCCGGA	1 cut(s)	2264
MroXI	GAANNNNTTC	2 cut(s)	933, 1357
MslI	CAYNNNNRTG	7 cut(s)	1131, 1240, 1489, 1562, 1641, 1724, 3054
MspA1I	CMGCKG	1 cut(s)	1072
MspCI	CTTAAG	1 cut(s)	3164
MspI	CCGG	2 cut(s)	1613, 2265
MspR9I	CCNGG	4 cut(s)	8, 2092, 2889, 3395
MunI	CAATTG	4 cut(s)	791, 1097, 1171, 2975
Mva1269I	GAATGC	4 cut(s)	196, 1702, 3285, 3343
MvaI	CCWGG	4 cut(s)	8, 2092, 2889, 3395
MvnI	CGCG	2 cut(s)	260, 1328
MwoI	GCNNNNNNNGC	6 cut(s)	191, 550, 809, 1525, 2590, 2624
NarI	GGCGCC	1 cut(s)	2971
NcoI	CCATGG	3 cut(s)	1224, 1636, 2119
NdeI	CATATG	3 cut(s)	665, 952, 3236
NlaIV	GGNNCC	6 cut(s)	285, 1885, 2190, 2330, 2600, 2972
NmeAIII	GCCGAG	1 cut(s)	2935
NmuCI	GTSAC	4 cut(s)	764, 1410, 1553, 2405
NsiI	ATGCAT	3 cut(s)	196, 1189, 1939
NspI	RCATGY	4 cut(s)	505, 852, 2467, 3137
PagI	TCATGA	1 cut(s)	943
PceI	AGGCCT	1 cut(s)	2095
PciI	ACATGT	2 cut(s)	501, 3133
PciSI	GCTCTTC	1 cut(s)	1509
PctI	GAATGC	4 cut(s)	196, 1702, 3285, 3343
PdmI	GAANNNNTTC	2 cut(s)	933, 1357
PflFI	GACNNNGTC	1 cut(s)	2411
PflMI	CCANNNNNTGG	1 cut(s)	2608
PluTI	GGCGCC	1 cut(s)	2974
PpuMI	RGGWCCY	1 cut(s)	283
PscI	ACATGT	2 cut(s)	501, 3133
PsiI	TTATAA	1 cut(s)	872
Psp5II	RGGWCCY	1 cut(s)	283
Psp6I	CCWGG	4 cut(s)	6, 2090, 2887, 3393
PspEI	GGTNACC	1 cut(s)	764
PspGI	CCWGG	4 cut(s)	6, 2090, 2887, 3393
PspN4I	GGNNCC	6 cut(s)	285, 1885, 2190, 2330, 2600, 2972
PspPI	GGNCC	7 cut(s)	252, 283, 586, 2214, 2329, 2865, 3544
PspPPI	RGGWCCY	1 cut(s)	283
PstI	CTGCAG	3 cut(s)	1471, 2502, 3294
PstNI	CAGNNNCTG	5 cut(s)	322, 1072, 2810, 2992, 3533
PsuI	RGATCY	4 cut(s)	55, 2161, 2989, 3190
PsyI	GACNNNGTC	1 cut(s)	2411
PvuII	CAGCTG	1 cut(s)	1072
RsaI	GTAC	7 cut(s)	825, 1105, 1230, 1453, 1820, 2600, 2774
RsaNI	GTAC	7 cut(s)	824, 1104, 1229, 1452, 1819, 2599, 2773
RseI	CAYNNNNRTG	7 cut(s)	1131, 1240, 1489, 1562, 1641, 1724, 3054
Rsr2I	CGGWCCG	1 cut(s)	252
RsrII	CGGWCCG	1 cut(s)	252
SapI	GCTCTTC	1 cut(s)	1509
Sau96I	GGNCC	7 cut(s)	252, 283, 586, 2214, 2329, 2865, 3544
ScrFI	CCNGG	4 cut(s)	8, 2092, 2889, 3395
SduI	GDGCHC	5 cut(s)	314, 551, 1530, 3207, 3301
SfcI	CTRYAG	8 cut(s)	754, 1467, 2193, 2209, 2498, 2814, 3264, 3290
SfoI	GGCGCC	1 cut(s)	2972
SinI	GGWCC	4 cut(s)	252, 283, 586, 3544
SmiMI	CAYNNNNRTG	7 cut(s)	1131, 1240, 1489, 1562, 1641, 1724, 3054
SmlI	CTYRAG	3 cut(s)	1529, 2711, 3164
SmoI	CTYRAG	3 cut(s)	1529, 2711, 3164
SseBI	AGGCCT	1 cut(s)	2095
SspDI	GGCGCC	1 cut(s)	2970
SspI	AATATT	1 cut(s)	331
SspMI	CTAG	3 cut(s)	1082, 1551, 3302
StuI	AGGCCT	1 cut(s)	2095
StyD4I	CCNGG	4 cut(s)	6, 2090, 2887, 3393
StyI	CCWWGG	4 cut(s)	1224, 1636, 2119, 2839
TaaI	ACNGT	7 cut(s)	581, 1349, 1624, 1800, 2013, 2336, 2413
TaiI	ACGT	3 cut(s)	1527, 1746, 2746
TaqI	TCGA	5 cut(s)	51, 308, 411, 679, 2854
TaqII	GACCGA	1 cut(s)	240
TatI	WGTACW	5 cut(s)	823, 1103, 1451, 1818, 2772
TauI	GCSGC	4 cut(s)	105, 263, 2453, 2800
TscAI	CASTG	8 cut(s)	586, 1354, 1471, 2018, 2341, 2416, 2799, 3299
TseFI	GTSAC	4 cut(s)	764, 1410, 1553, 2405
TseI	GCWGC	8 cut(s)	242, 348, 698, 1069, 1072, 3106, 3124, 3533
Tsp45I	GTSAC	4 cut(s)	764, 1410, 1553, 2405
TspGWI	ACGGA	5 cut(s)	108, 145, 432, 1301, 3529
TspRI	CASTG	8 cut(s)	586, 1354, 1471, 2018, 2341, 2416, 2799, 3299
Tth111I	GACNNNGTC	1 cut(s)	2411
Van91I	CCANNNNNTGG	1 cut(s)	2608
Vha464I	CTTAAG	1 cut(s)	3164
VneI	GTGCAC	1 cut(s)	3203
VpaK11BI	GGWCC	4 cut(s)	252, 283, 586, 3544
XagI	CCTNNNNNAGG	1 cut(s)	2498
XapI	RAATTY	6 cut(s)	380, 830, 917, 1301, 2697, 3358
XbaI	TCTAGA	1 cut(s)	3301
XceI	RCATGY	4 cut(s)	505, 852, 2467, 3137
XmiI	GTMKAC	2 cut(s)	785, 2859
XmnI	GAANNNNTTC	2 cut(s)	933, 1357
XspI	CTAG	3 cut(s)	1082, 1551, 3302
Zsp2I	ATGCAT	3 cut(s)	196, 1189, 1939

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.