Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003027

Physical Location & Seq

Genomic Coordinates Reverse (-)

19660558 .. 19671849

Gene Structure 11292 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1162300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1434 bp

ATGGATTCTGCCTCAAAGGTGAATGAGAGAATCATAGGGGCCTTAAAGGAAGCTACATTTAGAAAAAGAATTGAGACTAATAATTCTTTAATTGAAGAAGCCTCTGCAAAGAACCAACCTCTAAATGCAACATATACTTCTTCAGATTTATTGACATTACAACATTTGAAGGTTAACTCCCATAACAATTTTCTGAGCATGATCCCAATTCTGACAAAAACTAACATAATTCACATGATAACCAAGCCAGGAGAGAGAGACAGAACAGACTTTGGGGGTGCTTTACCTTACTTTGACTTTTCTTTCATAAAAGACCCTTTTAAGGTGTGTCTCCAAAAGCCACCTGCAGGGCTTTTGGACTCCAGTGCCTCAGCTTCCAGTGTCTGGAGGGATCACCTTGGAAAACAATGTTCTGGTGGAGATAATGTCTTGATTGATAAGACCAATGTATCTGATGTTCTTCCATATTCAGACTCAAAGGACCACAACCAGGCAGATGCAAATTTAACAAATGTTACTGGTGGTAGTTGTTGGGAGAGTTTGCTTGGTAGATTTAGTGACACTGTCGTTAGTAGAGTTGAAGATCACAGACAAGGCACGGGAGACAGTTTTGAGATACCACTTGATTTTATTATTGACAAATGCCTTCTGCAAGATGGTAACCTTAAACATGCTAGATATAAGTATGTCAGCAAGCTAACTATTAAGTTGCTTGAAGAAGGATTTGAATTGCAAGAACATTTACTGGCTCTACGGCGATACCACTTTATGGAACTAGCGGACTGGGCAGATTTGTTTATTATGTCTCTTTGGCAGCATAAATGGTGCGTTACAGAGGCAGATCATAGACTTTCAGAAATTCAAGGTTTCCTTGAGTCATCAGTTCAGAGGTCATCATGTGAACGCGACCCTCATAAGGACAGGTTATTTGTATACATGAAAGGACATGATGCAATGACTCTTTCTGCATCTGTTATTGATGTCCATTCCTTCAACTTTCTTGGTTTGGGTTACCGAGTGGACTGGCCCATAAGTATTGTTTTGAGTCCTGTGAAGCTTGCTATATTCTCATTGATTAATGTATGGCGCCCATTAAAGGATTTGGTATGCTCAATTAGTCAGGATATTGACTCTGAACAATATGATATGGGAGCAAGCCATTTTAATGCATTAGTGAAGATGAGGCACCAGGTCAATCATTTTGTATCTACATTACAGCAATATGTGGAATCACAGTTATCACATGTTTTATGGTGCAGGTTTCTGTACTCACTTAAACATCAGGTCAAAGATACGATGGATCTTCAGTCTGTACATATGAAATATTTAACAGATTCGCTTGACATTAAACAACCGAGAAAAAAGTTTGCACCAAGTGTGTACTTGCTGTCGGCTGCAAGATTTCTGGAAGCAATGCACAAGAAACTACACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000226 GO:0000278 GO:0000280 GO:0000923 GO:0000930 GO:0000931 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005813 GO:0005815 GO:0005816 GO:0005822 GO:0005825 GO:0005829 GO:0005856 GO:0006810 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007020 GO:0007049 GO:0007051 GO:0007052 GO:0007097 GO:0007098 GO:0007163 GO:0008017 GO:0008092 GO:0008150 GO:0008274 GO:0008275 GO:0008360 GO:0009987 GO:0015630 GO:0015631 GO:0016043 GO:0022402 GO:0022414 GO:0022603 GO:0022604 GO:0022607 GO:0031021 GO:0031023 GO:0031109 GO:0031122 GO:0032153 GO:0032155 GO:0032991 GO:0034453 GO:0034622 GO:0034631 GO:0043015 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044450 GO:0044464 GO:0044732 GO:0046785 GO:0046907 GO:0048285 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051179 GO:0051225 GO:0051234 GO:0051258 GO:0051298 GO:0051321 GO:0051415 GO:0051418 GO:0051640 GO:0051641 GO:0051647 GO:0051649 GO:0051656 GO:0061496 GO:0061497 GO:0065003 GO:0065007 GO:0065008 GO:0070925 GO:0071840 GO:0071957 GO:0071963 GO:0072393 GO:0090307 GO:0097435 GO:0098863 GO:0099098 GO:0099111 GO:0140014 GO:1902850 GO:1903047 GO:1990734

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Spc97_Spc98

Protein Analysis

478

Amino Acids

54.45

Weight (kDa)

6.74

Isoelectric Point (pI)

38.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GCP_C_terminal	PF04130	244 - 447	5.6e-34	Gamma tubulin complex component C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003027)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43610 AT3G43610 AT3G43610
fragaria_vesca	FvH4_3g27030 FvH4_3g27031 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990 FvH4_4g33990
malus_domestica	MD03G1162300.v1.1 MD03G1162400.v1.1 MD11G1178600.v1.1
prunus_persica	Prupe.6G151400_v2.0.a1 Prupe.6G151400_v2.0.a1 Prupe.6G151400_v2.0.a1
pyrus_communis	pycom11g15400
rosa_chinensis	RchiOBHm_Chr5g0049541
rosa_laevigata	RLG00000034625
rosa_multiflora	Rmu_co8383485.1_g000001 Rmu_sc0013032.1_g000004
rosa_roxburghii	Rroxscaffold_1G00032160
rosa_rugosa	Rorug05G0248900 Rorug05G0249000
rosa_samantha	Rh5AG325500 Rh5BG336700 Rh5CG361500 Rh5DG348800
rosa_wichuraiana	Rw5G030750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	352
Acc36I	ACCTGC	2 cut(s)	352, 1248
AccB1I	GGYRCC	2 cut(s)	1086, 1185
AccB7I	CCANNNNNTGG	2 cut(s)	384, 769
AccI	GTMKAC	1 cut(s)	933
AccII	CGCG	1 cut(s)	906
AciI	CCGC	1 cut(s)	779
AclWI	GGATC	3 cut(s)	196, 399, 1308
AcsI	RAATTY	2 cut(s)	502, 858
AcuI	CTGAAG	2 cut(s)	126, 1289
AcyI	GRCGYC	1 cut(s)	1087
AdeI	CACNNNGTG	1 cut(s)	1376
AfaI	GTAC	3 cut(s)	1268, 1314, 1382
AfiI	CCNNNNNNNGG	7 cut(s)	322, 347, 384, 490, 769, 916, 1096
AflIII	ACRYGT	1 cut(s)	1243
AgsI	TTSAA	7 cut(s)	95, 169, 581, 716, 728, 863, 994
AjnI	CCWGG	3 cut(s)	247, 489, 1188
AluBI	AGCT	4 cut(s)	53, 374, 697, 1057
AluI	AGCT	4 cut(s)	53, 374, 697, 1057
Alw26I	GTCTC	5 cut(s)	68, 252, 335, 597, 810
AlwI	GGATC	3 cut(s)	196, 399, 1308
AlwNI	CAGNNNCTG	1 cut(s)	384
AoxI	GGCC	2 cut(s)	39, 1025
ApeKI	GCWGC	2 cut(s)	814, 1394
ApoI	RAATTY	2 cut(s)	502, 858
ArsI	GACNNNNNNTTYG	2 cut(s)	911, 943
AseI	ATTAAT	1 cut(s)	1077
AspLEI	GCGC	1 cut(s)	1089
AspS9I	GGNCC	3 cut(s)	39, 481, 1026
AsuHPI	GGTGA	2 cut(s)	31, 386
AvaII	GGWCC	1 cut(s)	481
BaeI	ACNNNNGTAYC	2 cut(s)	432, 465
BanI	GGYRCC	2 cut(s)	1086, 1185
BbvCI	CCTCAGC	1 cut(s)	370
BbvI	GCAGC	2 cut(s)	826, 1381
BccI	CCATC	2 cut(s)	650, 1291
BceAI	ACGGC	1 cut(s)	770
BciT130I	CCWGG	3 cut(s)	249, 491, 1190
BcoDI	GTCTC	5 cut(s)	68, 252, 335, 597, 810
BfaI	CTAG	2 cut(s)	675, 776
BfmI	CTRYAG	1 cut(s)	345
BfoI	RGCGCY	1 cut(s)	1090
BfuAI	ACCTGC	2 cut(s)	352, 1248
BisI	GCNGC	2 cut(s)	815, 1395
BlsI	GCNGC	2 cut(s)	816, 1396
Bme1390I	CCNGG	3 cut(s)	249, 491, 1190
Bme18I	GGWCC	1 cut(s)	481
BmgT120I	GGNCC	3 cut(s)	39, 481, 1026
BmiI	GGNNCC	3 cut(s)	40, 1088, 1187
BmrFI	CCNGG	3 cut(s)	249, 491, 1190
BmrI	ACTGGG	1 cut(s)	793
BmsI	GCATC	3 cut(s)	487, 940, 977
BmuI	ACTGGG	1 cut(s)	793
BpmI	CTGGAG	2 cut(s)	346, 406
Bpu10I	CCTNAGC	1 cut(s)	370
BpuEI	CTTGAG	1 cut(s)	893
BsaHI	GRCGYC	1 cut(s)	1087
BsaJI	CCNNGG	1 cut(s)	397
BsaXI	ACNNNNNCTCC	2 cut(s)	411, 441
Bsc4I	CCNNNNNNNGG	7 cut(s)	322, 347, 384, 490, 769, 916, 1096
Bse1I	ACTGG	6 cut(s)	363, 378, 523, 750, 788, 1028
Bse3DI	GCAATG	2 cut(s)	960, 1419
BseBI	CCWGG	3 cut(s)	249, 491, 1190
BseDI	CCNNGG	1 cut(s)	397
BseLI	CCNNNNNNNGG	7 cut(s)	322, 347, 384, 490, 769, 916, 1096
BseMI	GCAATG	2 cut(s)	960, 1419
BseMII	CTCAG	2 cut(s)	185, 384
BseNI	ACTGG	6 cut(s)	363, 378, 523, 750, 788, 1028
BseXI	GCAGC	2 cut(s)	826, 1381
BsgI	GTGCAG	1 cut(s)	1276
Bsh1236I	CGCG	1 cut(s)	906
BshFI	GGCC	2 cut(s)	41, 1027
BshNI	GGYRCC	2 cut(s)	1086, 1185
BslI	CCNNNNNNNGG	7 cut(s)	322, 347, 384, 490, 769, 916, 1096
BsmAI	GTCTC	5 cut(s)	68, 252, 335, 597, 810
BsnI	GGCC	2 cut(s)	41, 1027
Bsp1407I	TGTACA	1 cut(s)	1312
Bsp143I	GATC	5 cut(s)	201, 391, 583, 841, 1300
BspACI	CCGC	1 cut(s)	779
BspANI	GGCC	2 cut(s)	41, 1027
BspCNI	CTCAG	2 cut(s)	186, 383
BspFNI	CGCG	1 cut(s)	906
BspLI	GGNNCC	3 cut(s)	40, 1088, 1187
BspMAI	CTGCAG	1 cut(s)	349
BspMI	ACCTGC	2 cut(s)	352, 1248
BspPI	GGATC	3 cut(s)	196, 399, 1308
BspT107I	GGYRCC	2 cut(s)	1086, 1185
BsrDI	GCAATG	2 cut(s)	960, 1419
BsrGI	TGTACA	1 cut(s)	1312
BsrI	ACTGG	6 cut(s)	363, 378, 523, 750, 788, 1028
BssECI	CCNNGG	1 cut(s)	397
BssMI	GATC	5 cut(s)	201, 391, 583, 841, 1300
BssNAI	GTATAC	1 cut(s)	934
BssNI	GRCGYC	1 cut(s)	1087
BssT1I	CCWWGG	1 cut(s)	397
Bst1107I	GTATAC	1 cut(s)	934
Bst2UI	CCWGG	3 cut(s)	249, 491, 1190
Bst4CI	ACNGT	3 cut(s)	565, 608, 1236
BstACI	GRCGYC	1 cut(s)	1087
BstAUI	TGTACA	1 cut(s)	1312
BstC8I	GCNNGC	3 cut(s)	695, 1059, 1156
BstDEI	CTNAG	2 cut(s)	194, 370
BstEII	GGTNACC	2 cut(s)	659, 1010
BstFNI	CGCG	1 cut(s)	906
BstH2I	RGCGCY	1 cut(s)	1090
BstHHI	GCGC	1 cut(s)	1089
BstKTI	GATC	5 cut(s)	204, 394, 586, 844, 1303
BstMAI	GTCTC	5 cut(s)	68, 252, 335, 597, 810
BstMBI	GATC	5 cut(s)	201, 391, 583, 841, 1300
BstMWI	GCNNNNNNNGC	1 cut(s)	785
BstNI	CCWGG	3 cut(s)	249, 491, 1190
BstNSI	RCATGY	2 cut(s)	674, 1247
BstPI	GGTNACC	2 cut(s)	659, 1010
BstSCI	CCNGG	3 cut(s)	247, 489, 1188
BstSFI	CTRYAG	1 cut(s)	345
BstUI	CGCG	1 cut(s)	906
BstV1I	GCAGC	2 cut(s)	826, 1381
BstX2I	RGATCY	1 cut(s)	1300
BstYI	RGATCY	1 cut(s)	1300
BstZ17I	GTATAC	1 cut(s)	934
BsuRI	GGCC	2 cut(s)	41, 1027
BtsIMutI	CAGTG	4 cut(s)	370, 385, 561, 1429
BveI	ACCTGC	2 cut(s)	352, 1248
Cac8I	GCNNGC	3 cut(s)	695, 1059, 1156
CaiI	CAGNNNCTG	1 cut(s)	384
CfoI	GCGC	1 cut(s)	1089
Cfr13I	GGNCC	3 cut(s)	39, 481, 1026
CsiI	ACCWGGT	1 cut(s)	1188
Csp6I	GTAC	3 cut(s)	1267, 1313, 1381
CviAII	CATG	7 cut(s)	199, 235, 671, 897, 937, 947, 1244
CviQI	GTAC	3 cut(s)	1267, 1313, 1381
DdeI	CTNAG	2 cut(s)	194, 370
DinI	GGCGCC	1 cut(s)	1088
DpnI	GATC	5 cut(s)	203, 393, 585, 843, 1302
DpnII	GATC	5 cut(s)	201, 391, 583, 841, 1300
DraIII	CACNNNGTG	1 cut(s)	1376
Eco130I	CCWWGG	1 cut(s)	397
Eco47I	GGWCC	1 cut(s)	481
Eco57I	CTGAAG	2 cut(s)	126, 1289
Eco91I	GGTNACC	2 cut(s)	659, 1010
EcoO109I	RGGNCCY	1 cut(s)	39
EcoO65I	GGTNACC	2 cut(s)	659, 1010
EcoRII	CCWGG	3 cut(s)	247, 489, 1188
EcoT14I	CCWWGG	1 cut(s)	397
EcoT22I	ATGCAT	1 cut(s)	1171
EgeI	GGCGCC	1 cut(s)	1088
EheI	GGCGCC	1 cut(s)	1088
ErhI	CCWWGG	1 cut(s)	397
FaeI	CATG	7 cut(s)	202, 238, 674, 900, 940, 950, 1247
FalI	AAGNNNNNCTT	2 cut(s)	855, 887
FatI	CATG	7 cut(s)	198, 234, 670, 896, 936, 946, 1243
FauNDI	CATATG	1 cut(s)	1317
FblI	GTMKAC	1 cut(s)	933
Fnu4HI	GCNGC	2 cut(s)	815, 1395
Fsp4HI	GCNGC	2 cut(s)	815, 1395
FspBI	CTAG	2 cut(s)	675, 776
GlaI	GCGC	1 cut(s)	1088
GluI	GCNGC	2 cut(s)	815, 1395
GsuI	CTGGAG	2 cut(s)	346, 406
HaeII	RGCGCY	1 cut(s)	1090
HaeIII	GGCC	2 cut(s)	41, 1027
HhaI	GCGC	1 cut(s)	1089
Hin1I	GRCGYC	1 cut(s)	1087
Hin1II	CATG	7 cut(s)	202, 238, 674, 900, 940, 950, 1247
Hin6I	GCGC	1 cut(s)	1087
HinP1I	GCGC	1 cut(s)	1087
HincII	GTYRAC	1 cut(s)	175
HindII	GTYRAC	1 cut(s)	175
HindIII	AAGCTT	1 cut(s)	1055
HpaI	GTTAAC	1 cut(s)	175
HphI	GGTGA	2 cut(s)	31, 386
Hpy166II	GTNNAC	5 cut(s)	175, 902, 934, 1021, 1381
Hpy188I	TCNGA	8 cut(s)	145, 195, 213, 454, 472, 856, 888, 1135
Hpy188III	TCNNGA	4 cut(s)	385, 430, 1121, 1406
Hpy8I	GTNNAC	5 cut(s)	175, 902, 934, 1021, 1381
HpyAV	CCTTC	4 cut(s)	163, 656, 713, 1000
HpyCH4III	ACNGT	3 cut(s)	565, 608, 1236
HpyF10VI	GCNNNNNNNGC	1 cut(s)	785
HpyF3I	CTNAG	2 cut(s)	194, 370
Hsp92I	GRCGYC	1 cut(s)	1087
Hsp92II	CATG	7 cut(s)	202, 238, 674, 900, 940, 950, 1247
HspAI	GCGC	1 cut(s)	1087
KasI	GGCGCC	1 cut(s)	1086
KspAI	GTTAAC	1 cut(s)	175
Kzo9I	GATC	5 cut(s)	201, 391, 583, 841, 1300
LmnI	GCTCC	1 cut(s)	1151
Lsp1109I	GCAGC	2 cut(s)	826, 1381
LweI	GCATC	3 cut(s)	487, 940, 977
MabI	ACCWGGT	1 cut(s)	1188
MaeI	CTAG	2 cut(s)	675, 776
MaeIII	GTNAC	5 cut(s)	514, 557, 659, 829, 1010
MalI	GATC	5 cut(s)	203, 393, 585, 843, 1302
MboI	GATC	5 cut(s)	201, 391, 583, 841, 1300
MboII	GAAGA	7 cut(s)	107, 132, 452, 593, 728, 1189, 1295
MflI	RGATCY	1 cut(s)	1300
Mly113I	GGCGCC	1 cut(s)	1087
MlyI	GAGTC	6 cut(s)	353, 467, 884, 952, 1054, 1124
MnlI	CCTC	9 cut(s)	22, 112, 129, 379, 381, 829, 882, 921, 1176
Mph1103I	ATGCAT	1 cut(s)	1171
MslI	CAYNNNNRTG	1 cut(s)	1164
MspR9I	CCNGG	3 cut(s)	249, 491, 1190
MvaI	CCWGG	3 cut(s)	249, 491, 1190
MvnI	CGCG	1 cut(s)	906
MwoI	GCNNNNNNNGC	1 cut(s)	785
NarI	GGCGCC	1 cut(s)	1087
NdeI	CATATG	1 cut(s)	1317
NdeII	GATC	5 cut(s)	201, 391, 583, 841, 1300
NlaIII	CATG	7 cut(s)	202, 238, 674, 900, 940, 950, 1247
NlaIV	GGNNCC	3 cut(s)	40, 1088, 1187
NmuCI	GTSAC	1 cut(s)	557
NsiI	ATGCAT	1 cut(s)	1171
NspI	RCATGY	2 cut(s)	674, 1247
PaqCI	CACCTGC	1 cut(s)	352
PciI	ACATGT	1 cut(s)	1243
PfeI	GAWTC	4 cut(s)	5, 30, 1229, 1334
PflFI	GACNNNGTC	1 cut(s)	563
PflMI	CCANNNNNTGG	2 cut(s)	384, 769
PkrI	GCNGC	2 cut(s)	816, 1396
PleI	GAGTC	6 cut(s)	353, 467, 883, 952, 1053, 1124
PluTI	GGCGCC	1 cut(s)	1090
PpsI	GAGTC	6 cut(s)	353, 467, 883, 952, 1053, 1124
PscI	ACATGT	1 cut(s)	1243
PshBI	ATTAAT	1 cut(s)	1077
Psp6I	CCWGG	3 cut(s)	247, 489, 1188
PspEI	GGTNACC	2 cut(s)	659, 1010
PspGI	CCWGG	3 cut(s)	247, 489, 1188
PspN4I	GGNNCC	3 cut(s)	40, 1088, 1187
PspPI	GGNCC	3 cut(s)	39, 481, 1026
PsrI	GAACNNNNNNTAC	2 cut(s)	441, 473
PstI	CTGCAG	1 cut(s)	349
PstNI	CAGNNNCTG	1 cut(s)	384
PsuI	RGATCY	1 cut(s)	1300
PsyI	GACNNNGTC	1 cut(s)	563
RsaI	GTAC	3 cut(s)	1268, 1314, 1382
RsaNI	GTAC	3 cut(s)	1267, 1313, 1381
RseI	CAYNNNNRTG	1 cut(s)	1164
SatI	GCNGC	2 cut(s)	815, 1395
Sau3AI	GATC	5 cut(s)	201, 391, 583, 841, 1300
Sau96I	GGNCC	3 cut(s)	39, 481, 1026
SbfI	CCTGCAGG	1 cut(s)	349
SchI	GAGTC	6 cut(s)	353, 467, 884, 952, 1054, 1124
ScrFI	CCNGG	3 cut(s)	249, 491, 1190
SdaI	CCTGCAGG	1 cut(s)	349
SexAI	ACCWGGT	1 cut(s)	1188
SfaNI	GCATC	3 cut(s)	487, 940, 977
SfcI	CTRYAG	1 cut(s)	345
SfoI	GGCGCC	1 cut(s)	1088
SinI	GGWCC	1 cut(s)	481
SmiMI	CAYNNNNRTG	1 cut(s)	1164
SmlI	CTYRAG	1 cut(s)	872
SmoI	CTYRAG	1 cut(s)	872
Sse8387I	CCTGCAGG	1 cut(s)	349
SsiI	CCGC	1 cut(s)	779
SspDI	GGCGCC	1 cut(s)	1086
SspI	AATATT	1 cut(s)	1325
SspMI	CTAG	2 cut(s)	675, 776
StyD4I	CCNGG	3 cut(s)	247, 489, 1188
StyI	CCWWGG	1 cut(s)	397
TaaI	ACNGT	3 cut(s)	565, 608, 1236
TatI	WGTACW	3 cut(s)	1266, 1312, 1380
TfiI	GAWTC	4 cut(s)	5, 30, 1229, 1334
TscAI	CASTG	3 cut(s)	370, 385, 568
TseFI	GTSAC	1 cut(s)	557
TseI	GCWGC	2 cut(s)	814, 1394
Tsp45I	GTSAC	1 cut(s)	557
TspDTI	ATGAA	3 cut(s)	295, 953, 1334
TspRI	CASTG	3 cut(s)	370, 385, 568
Tth111I	GACNNNGTC	1 cut(s)	563
Van91I	CCANNNNNTGG	2 cut(s)	384, 769
VpaK11BI	GGWCC	1 cut(s)	481
VspI	ATTAAT	1 cut(s)	1077
XapI	RAATTY	2 cut(s)	502, 858
XceI	RCATGY	2 cut(s)	674, 1247
XmiI	GTMKAC	1 cut(s)	933
XspI	CTAG	2 cut(s)	675, 776
Zsp2I	ATGCAT	1 cut(s)	1171

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.