FvH4_4g34510

pre-mRNA-processing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004435
Physical Location & Seq
Forward (+)
32248802 .. 32251525
2724 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g34510.t1

Sequence Viewer

Length: 1746 bp
ATGGATCTCCTCCGAGCTTACTCCGACGACCAAAACCCTAACTCCCCAGCCTCCTCCCCCTCACCGGAATCTTCACCTCCACGTCTCCTCCCTCCTAAATCCGCCGCGCCCCAAGTCGACGACACCATGCTCGCACTCACCGTCGCGAAGAATCGCAGCGCAATGACCCGCCCCATCGACCCGACCCAGCACCTCGTCGACTTCAACCCGACCTACGACCAGCTCTGGGCCCCAATCTACGGCCCCTCCCACCCCTACGCCAAGGACGGCCTCGCCCAAGGGATGCGCAACCACAAGCTAGGGTTTGTCGAGGACGCCGCCATCGAGCCTTTCATCTTCGACGAGCAGTACAACACTTTCCAGAACTACGGCTACGCCGCCGACCCCTCCGCCTCCGCGGGTTACAACTACGTCGGCGATATGACCGCATTGCAGAAAAACGACGCCGTTTCGGTGTACAACATCCCCCAGCACGAGCAGAAGAAGCGGAAGATCGAGAAGCGGAAGGAGAATGACGAGGACGGAGACGACAACGCCGAGATGGTGGACCCGGAGGAGGTGGCGAATCCGGCCAGCGAGGCGTGGCTGATGAAGAACAGGAAGAGCCCCTGGGCCGGGAAGAAGGAGGGCCCGCCGGTGGAGCTCACGGAGGAGCAGAAGAAGTACGCGGAGGAGTATGCGAAGAAGAAAGGTGAGGAGAAAGGCGGCGGCGACAAGGGAGAGGCTGTGGTGGAGAAGACTACTTTCCACGGAAAAGAAGAGAGGGACTATCAAGGGAGGTCCTGGATTGCGCAGCCCAAGGATGCCAAGGCTAGTAATGATCACTGTTATATTCCGAAACGGCTTGTGCATACTTGGAGTGGTCACACTAAGGGTGTGAATGCTATTAGGTTCTTCCCGAACTCGGGGCATTTGATACTCTCGGCAGGGTTGGACACTAAGTGCAAGATTTGGGATGTGTCGAATACTGGCAAGTGCATGAGGACTTACATGGGGCATTCTAAGGCTGTTAGGGATATTTGTTTCTCGAATGATGGGAGTAAGTTTTTGAGTGCTGGCTATGATAAGAATATCAAGTATTGGGATACGGAGACTGGGCAGGTGATATCTACATTTTCGACTGGGAAGGTGCCTTATGTGGTGAAGCTTAATCCGGATGATGATAAGCAGAATGTGTTGTTAGCTGGGATGAGTGATAAGAAGATTGTTCAGTGGGATATGAATGAGGGGAAGATTACACAAGAGTATGATCAGCATTTGGGGGCGGTGAATACTATTACGTTTGTTGATAATAATCGGAGGTTTGTTACTTCCAGTGATGACAAGTCTCTGCGTGTTTGGGAGTTTGGGATTCCTGTGGTGATCAAGTATATTAGTGAGCCTCATATGCATTCTATGCCATCGATTTCGTTGCACCCCAATTCGAATTGGCTTGCAGCACAGAGTATGGACAATCAGATTCTTATCTACAGCACTAGGGAGAAGTTTCAGCTCAATAAGAAGAAGAGGTTTGCAGGACATGTTGTAGCTGGGTATGCTTGCCAAGTAAATTTCTCGCCAGACGGACGATTTGTTTTCTCGGGTGATGGTGAAGGTAGATGCTGGTTTTGGGATTGGAAGTCTTGCAAAGTCTTCAGAACTCTCAAATGTCATGAGGGGGTGTGCATTGGGGCTGCGTGGCATCCCTTGGAGCAAAGTAAAGTCGCAACTTGTGGCTGGGATGGCTTGATTAAATATTGGGACTAG

Protein Analysis

582

Amino Acids

65.5

Weight (kDa)

6.51

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40 PF00400 281 - 319 4.8e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 282 - 387 4.4e-17 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 282 - 374 5.3e-16 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 282 - 448 1.6e-09 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 282 - 447 5.3e-17 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 285 - 449 4.8e-11 TEP-1 second beta-propeller
WD40_Gbeta PF25391 285 - 352 7.2e-06 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 286 - 580 4.3e-51 WDR5 beta-propeller domain
EIF3I PF24805 287 - 388 2.5e-12 EIF3I
Beta-prop_WDR3_2nd PF25172 288 - 373 5.3e-10 WDR3 second beta-propeller domain
Beta-prop_EML PF23409 289 - 491 2.2e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 289 - 374 7.3e-12 Prp19 WD40 domain
WDR55 PF24796 292 - 449 1e-17 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 319 - 388 1e-10 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 324 - 362 8.3e-10 WD domain, G-beta repeat
Beta-prop_WDR41 PF25178 329 - 453 7.9e-07 WDR41
WD40_RFWD3 PF23419 333 - 408 1.1e-07 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Beta-prop_EML_2 PF23414 348 - 448 1.3e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 350 - 448 2.3e-10 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_1st PF25171 364 - 449 6.2e-09 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 380 - 555 6.5e-23 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 387 - 491 7.4e-12 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 389 - 575 5.8e-15 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 392 - 497 2.2e-07 WDR36/Utp21 second beta-propeller domain
EIF3I PF24805 392 - 553 7.6e-08 EIF3I
Beta-prop_THOC3 PF25174 392 - 581 2.8e-23 THOC3 beta-propeller domain
WDR90_beta-prop_4th PF23342 423 - 557 6.3e-06 WDR90, 4th beta-propeller
WD40_CDC20-Fz PF24807 460 - 581 9.7e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 462 - 581 2.2e-09 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 464 - 545 1.1e-07 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 467 - 581 5.7e-08 WDR55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1090
Acc16I TGCGCA 2 cut(s) 287, 792
Acc36I ACCTGC 1 cut(s) 1090
AccB1I GGYRCC 1 cut(s) 1129
AccI GTMKAC 2 cut(s) 117, 198
AccII CGCG 4 cut(s) 107, 146, 398, 668
AccIII TCCGGA 1 cut(s) 1153
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 570
AcsI RAATTY 1 cut(s) 1549
AcuI CTGAAG 1 cut(s) 1618
AcyI GRCGYC 2 cut(s) 315, 444
AdeI CACNNNGTG 1 cut(s) 942
AfaI GTAC 3 cut(s) 350, 458, 665
AfiI CCNNNNNNNGG 9 cut(s) 64, 226, 239, 556, 614, 615, 637, 904, 905
AflIII ACRYGT 1 cut(s) 1519
AgsI TTSAA 1 cut(s) 205
AjiI CACGTC 1 cut(s) 83
AjnI CCWGG 2 cut(s) 608, 782
AluBI AGCT 8 cut(s) 17, 223, 298, 643, 1147, 1184, 1492, 1529
AluI AGCT 8 cut(s) 17, 223, 298, 643, 1147, 1184, 1492, 1529
Alw21I GWGCWC 1 cut(s) 645
Alw26I GTCTC 4 cut(s) 89, 519, 1085, 1332
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 2 cut(s) 904, 1579
Aor13HI TCCGGA 1 cut(s) 1153
AoxI GGCC 6 cut(s) 228, 241, 268, 570, 612, 628
ApaI GGGCCC 2 cut(s) 232, 632
ApeKI GCWGC 4 cut(s) 156, 793, 1436, 1673
ApoI RAATTY 1 cut(s) 1549
ArsI GACNNNNNNTTYG 2 cut(s) 1553, 1585
AspLEI GCGC 4 cut(s) 109, 161, 288, 793
AspS9I GGNCC 8 cut(s) 228, 229, 242, 547, 612, 628, 629, 780
AsuC2I CCSGG 2 cut(s) 551, 616
AsuII TTCGAA 1 cut(s) 1424
AvaI CYCGRG 2 cut(s) 904, 1579
AvaII GGWCC 2 cut(s) 547, 780
BaeGI GKGCMC 2 cut(s) 232, 632
BanI GGYRCC 1 cut(s) 1129
BanII GRGCYC 4 cut(s) 232, 608, 632, 645
BauI CACGAG 1 cut(s) 473
BbsI GAAGAC 2 cut(s) 743, 1624
Bbv12I GWGCWC 1 cut(s) 645
BbvI GCAGC 4 cut(s) 168, 805, 1448, 1660
BccI CCATC 7 cut(s) 182, 329, 535, 1028, 1408, 1580, 1715
BceAI ACGGC 5 cut(s) 256, 283, 385, 431, 857
BcgI CGANNNNNNTGC 2 cut(s) 1393, 1427
BciT130I CCWGG 2 cut(s) 610, 784
BciVI GTATCC 1 cut(s) 1078
BclI TGATCA 3 cut(s) 820, 1249, 1362
BcnI CCSGG 2 cut(s) 551, 616
BcoDI GTCTC 4 cut(s) 89, 519, 1085, 1332
BfaI CTAG 4 cut(s) 299, 813, 1476, 1744
BfmI CTRYAG 1 cut(s) 1468
BfuAI ACCTGC 1 cut(s) 1090
BfuI GTATCC 1 cut(s) 1078
BglI GCCNNNNNGGC 1 cut(s) 578
BisI GCNGC 9 cut(s) 105, 157, 318, 378, 706, 709, 794, 1437, 1674
BlsI GCNGC 9 cut(s) 106, 158, 319, 379, 707, 710, 795, 1438, 1675
Bme1390I CCNGG 4 cut(s) 551, 610, 616, 784
Bme18I GGWCC 2 cut(s) 547, 780
BmeT110I CYCGRG 2 cut(s) 904, 1579
BmgBI CACGTC 1 cut(s) 83
BmgT120I GGNCC 8 cut(s) 228, 229, 242, 547, 612, 628, 629, 780
BmiI GGNNCC 6 cut(s) 230, 231, 244, 549, 630, 1131
BmrFI CCNGG 4 cut(s) 551, 610, 616, 784
BmrI ACTGGG 2 cut(s) 1104, 1131
BmsI GCATC 4 cut(s) 273, 793, 1589, 1690
BmuI ACTGGG 2 cut(s) 1104, 1131
BpiI GAAGAC 2 cut(s) 743, 1624
Bpu14I TTCGAA 1 cut(s) 1424
BpuMI CCSGG 2 cut(s) 551, 616
Bsa29I ATCGAT 1 cut(s) 1403
BsaBI GATNNNNATC 2 cut(s) 1293, 1463
BsaHI GRCGYC 2 cut(s) 315, 444
BsaJI CCNNGG 9 cut(s) 261, 277, 396, 608, 609, 748, 798, 807, 1686
BsaWI WCCGGW 2 cut(s) 64, 1153
BsaXI ACNNNNNCTCC 8 cut(s) 26, 56, 72, 102, 230, 260, 711, 741
Bsc4I CCNNNNNNNGG 9 cut(s) 64, 226, 239, 556, 614, 615, 637, 904, 905
Bse118I RCCGGY 1 cut(s) 634
Bse1I ACTGG 4 cut(s) 973, 1099, 1126, 1314
Bse3DI GCAATG 2 cut(s) 168, 428
Bse8I GATNNNNATC 2 cut(s) 1293, 1463
BseAI TCCGGA 1 cut(s) 1153
BseBI CCWGG 2 cut(s) 610, 784
BseCI ATCGAT 1 cut(s) 1403
BseDI CCNNGG 9 cut(s) 261, 277, 396, 608, 609, 748, 798, 807, 1686
BseGI GGATG 8 cut(s) 288, 462, 808, 961, 1162, 1194, 1681, 1726
BseJI GATNNNNATC 2 cut(s) 1293, 1463
BseLI CCNNNNNNNGG 9 cut(s) 64, 226, 239, 556, 614, 615, 637, 904, 905
BseMI GCAATG 2 cut(s) 168, 428
BseNI ACTGG 4 cut(s) 973, 1099, 1126, 1314
BseRI GAGGAG 6 cut(s) 43, 77, 569, 665, 686, 710
BseSI GKGCMC 2 cut(s) 232, 632
BseXI GCAGC 4 cut(s) 168, 805, 1448, 1660
BseYI CCCAGC 6 cut(s) 46, 186, 468, 1184, 1529, 1716
Bsh1236I CGCG 4 cut(s) 107, 146, 398, 668
BshFI GGCC 6 cut(s) 230, 243, 270, 572, 614, 630
BshNI GGYRCC 1 cut(s) 1129
BshVI ATCGAT 1 cut(s) 1403
BsiHKAI GWGCWC 1 cut(s) 645
BsiHKCI CYCGRG 2 cut(s) 904, 1579
BsiSI CCGG 6 cut(s) 65, 551, 569, 615, 635, 1154
BslFI GGGAC 1 cut(s) 779
BslI CCNNNNNNNGG 9 cut(s) 64, 226, 239, 556, 614, 615, 637, 904, 905
BsmAI GTCTC 4 cut(s) 89, 519, 1085, 1332
BsmBI CGTCTC 2 cut(s) 89, 519
BsmFI GGGAC 1 cut(s) 779
BsmI GAATGC 3 cut(s) 886, 997, 1390
BsnI GGCC 6 cut(s) 230, 243, 270, 572, 614, 630
BsoBI CYCGRG 2 cut(s) 904, 1579
Bsp119I TTCGAA 1 cut(s) 1424
Bsp120I GGGCCC 2 cut(s) 228, 628
Bsp1286I GDGCHC 4 cut(s) 232, 608, 632, 645
Bsp13I TCCGGA 1 cut(s) 1153
Bsp1407I TGTACA 1 cut(s) 456
Bsp143I GATC 5 cut(s) 4, 492, 820, 1249, 1362
Bsp68I TCGCGA 1 cut(s) 146
BspANI GGCC 6 cut(s) 230, 243, 270, 572, 614, 630
BspDI ATCGAT 1 cut(s) 1403
BspEI TCCGGA 1 cut(s) 1153
BspFNI CGCG 4 cut(s) 107, 146, 398, 668
BspHI TCATGA 1 cut(s) 1651
BspLI GGNNCC 6 cut(s) 230, 231, 244, 549, 630, 1131
BspMI ACCTGC 1 cut(s) 1090
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 596
BspT104I TTCGAA 1 cut(s) 1424
BspT107I GGYRCC 1 cut(s) 1129
BsrDI GCAATG 2 cut(s) 168, 428
BsrFI RCCGGY 1 cut(s) 634
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 4 cut(s) 973, 1099, 1126, 1314
BssAI RCCGGY 1 cut(s) 634
BssECI CCNNGG 9 cut(s) 261, 277, 396, 608, 609, 748, 798, 807, 1686
BssMI GATC 5 cut(s) 4, 492, 820, 1249, 1362
BssNI GRCGYC 2 cut(s) 315, 444
BssSI CACGAG 1 cut(s) 473
BssT1I CCWWGG 5 cut(s) 261, 277, 798, 807, 1686
Bst2BI CACGAG 1 cut(s) 473
Bst2UI CCWGG 2 cut(s) 610, 784
Bst4CI ACNGT 2 cut(s) 142, 827
Bst6I CTCTTC 3 cut(s) 596, 753, 1499
BstACI GRCGYC 2 cut(s) 315, 444
BstAPI GCANNNNNTGC 1 cut(s) 1396
BstAUI TGTACA 1 cut(s) 456
BstBI TTCGAA 1 cut(s) 1424
BstC8I GCNNGC 6 cut(s) 132, 574, 632, 1057, 1434, 1540
BstDEI CTNAG 3 cut(s) 870, 939, 1002
BstDSI CCRYGG 2 cut(s) 396, 748
BstF5I GGATG 8 cut(s) 288, 462, 808, 961, 1162, 1194, 1681, 1726
BstFNI CGCG 4 cut(s) 107, 146, 398, 668
BstHHI GCGC 4 cut(s) 109, 161, 288, 793
BstKTI GATC 5 cut(s) 7, 495, 823, 1252, 1365
BstMAI GTCTC 4 cut(s) 89, 519, 1085, 1332
BstMBI GATC 5 cut(s) 4, 492, 820, 1249, 1362
BstMWI GCNNNNNNNGC 8 cut(s) 484, 569, 578, 640, 1387, 1396, 1535, 1722
BstNI CCWGG 2 cut(s) 610, 784
BstNSI RCATGY 1 cut(s) 1523
BstSCI CCNGG 4 cut(s) 549, 608, 614, 782
BstSFI CTRYAG 1 cut(s) 1468
BstSLI GKGCMC 2 cut(s) 232, 632
BstUI CGCG 4 cut(s) 107, 146, 398, 668
BstV1I GCAGC 4 cut(s) 168, 805, 1448, 1660
BstV2I GAAGAC 2 cut(s) 743, 1624
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
Bsu15I ATCGAT 1 cut(s) 1403
BsuI GTATCC 1 cut(s) 1078
BsuRI GGCC 6 cut(s) 230, 243, 270, 572, 614, 630
BsuTUI ATCGAT 1 cut(s) 1403
BtgI CCRYGG 2 cut(s) 396, 748
BtrI CACGTC 1 cut(s) 83
BtsCI GGATG 8 cut(s) 288, 462, 808, 961, 1162, 1194, 1681, 1726
BtsIMutI CAGTG 3 cut(s) 823, 1217, 1321
BtuMI TCGCGA 1 cut(s) 146
BveI ACCTGC 1 cut(s) 1090
Cac8I GCNNGC 6 cut(s) 132, 574, 632, 1057, 1434, 1540
CciI TCATGA 1 cut(s) 1651
CfoI GCGC 4 cut(s) 109, 161, 288, 793
Cfr10I RCCGGY 1 cut(s) 634
Cfr13I GGNCC 8 cut(s) 228, 229, 242, 547, 612, 628, 629, 780
Cfr42I CCGCGG 1 cut(s) 399
ClaI ATCGAT 1 cut(s) 1403
CseI GACGC 2 cut(s) 323, 452
Csp6I GTAC 3 cut(s) 349, 457, 664
CviAII CATG 5 cut(s) 127, 979, 991, 1520, 1652
CviQI GTAC 3 cut(s) 349, 457, 664
DdeI CTNAG 3 cut(s) 870, 939, 1002
DpnI GATC 5 cut(s) 6, 494, 822, 1251, 1364
DpnII GATC 5 cut(s) 4, 492, 820, 1249, 1362
DraIII CACNNNGTG 1 cut(s) 942
EaeI YGGCCR 1 cut(s) 570
Eam1104I CTCTTC 3 cut(s) 596, 753, 1499
EarI CTCTTC 3 cut(s) 596, 753, 1499
EciI GGCGGA 2 cut(s) 91, 379
Ecl136II GAGCTC 1 cut(s) 643
Eco130I CCWWGG 5 cut(s) 261, 277, 798, 807, 1686
Eco24I GRGCYC 4 cut(s) 232, 608, 632, 645
Eco32I GATATC 1 cut(s) 1107
Eco47I GGWCC 2 cut(s) 547, 780
Eco53kI GAGCTC 1 cut(s) 643
Eco57I CTGAAG 1 cut(s) 1618
Eco88I CYCGRG 2 cut(s) 904, 1579
EcoICRI GAGCTC 1 cut(s) 643
EcoO109I RGGNCCY 3 cut(s) 229, 628, 780
EcoRII CCWGG 2 cut(s) 608, 782
EcoRV GATATC 1 cut(s) 1107
EcoT14I CCWWGG 5 cut(s) 261, 277, 798, 807, 1686
EcoT22I ATGCAT 1 cut(s) 1392
EcoT38I GRGCYC 4 cut(s) 232, 608, 632, 645
ErhI CCWWGG 5 cut(s) 261, 277, 798, 807, 1686
Esp3I CGTCTC 2 cut(s) 89, 519
FaeI CATG 5 cut(s) 130, 982, 994, 1523, 1655
FaqI GGGAC 1 cut(s) 779
FatI CATG 5 cut(s) 126, 978, 990, 1519, 1651
FauI CCCGC 3 cut(s) 176, 391, 639
FauNDI CATATG 1 cut(s) 1386
FbaI TGATCA 3 cut(s) 820, 1249, 1362
FblI GTMKAC 2 cut(s) 117, 198
Fnu4HI GCNGC 9 cut(s) 105, 157, 318, 378, 706, 709, 794, 1437, 1674
FokI GGATG 8 cut(s) 295, 449, 815, 968, 1169, 1201, 1668, 1733
FriOI GRGCYC 4 cut(s) 232, 608, 632, 645
Fsp4HI GCNGC 9 cut(s) 105, 157, 318, 378, 706, 709, 794, 1437, 1674
FspBI CTAG 4 cut(s) 299, 813, 1476, 1744
FspI TGCGCA 2 cut(s) 287, 792
GlaI GCGC 4 cut(s) 108, 160, 287, 792
GluI GCNGC 9 cut(s) 105, 157, 318, 378, 706, 709, 794, 1437, 1674
GsaI CCCAGC 6 cut(s) 50, 190, 472, 1188, 1533, 1720
HaeIII GGCC 6 cut(s) 230, 243, 270, 572, 614, 630
HapII CCGG 6 cut(s) 65, 551, 569, 615, 635, 1154
HgaI GACGC 2 cut(s) 323, 452
HhaI GCGC 4 cut(s) 109, 161, 288, 793
Hin1I GRCGYC 2 cut(s) 315, 444
Hin1II CATG 5 cut(s) 130, 982, 994, 1523, 1655
Hin6I GCGC 4 cut(s) 107, 159, 286, 791
HinP1I GCGC 4 cut(s) 107, 159, 286, 791
HincII GTYRAC 2 cut(s) 118, 199
HindII GTYRAC 2 cut(s) 118, 199
HindIII AAGCTT 1 cut(s) 1145
HinfI GANTC 5 cut(s) 68, 151, 565, 1351, 1459
HpaII CCGG 6 cut(s) 65, 551, 569, 615, 635, 1154
Hpy166II GTNNAC 4 cut(s) 118, 199, 457, 547
Hpy188I TCNGA 6 cut(s) 14, 25, 837, 1299, 1458, 1637
Hpy188III TCNNGA 7 cut(s) 145, 361, 496, 898, 1027, 1154, 1652
Hpy8I GTNNAC 4 cut(s) 118, 199, 457, 547
Hpy99I CGWCG 7 cut(s) 29, 122, 146, 200, 344, 416, 446
HpyAV CCTTC 4 cut(s) 499, 616, 1120, 1586
HpyCH4III ACNGT 2 cut(s) 142, 827
HpyCH4IV ACGT 3 cut(s) 82, 411, 1280
HpyF10VI GCNNNNNNNGC 8 cut(s) 484, 569, 578, 640, 1387, 1396, 1535, 1722
HpyF3I CTNAG 3 cut(s) 870, 939, 1002
HpySE526I ACGT 3 cut(s) 82, 411, 1280
Hsp92I GRCGYC 2 cut(s) 315, 444
Hsp92II CATG 5 cut(s) 130, 982, 994, 1523, 1655
HspAI GCGC 4 cut(s) 107, 159, 286, 791
Kpn2I TCCGGA 1 cut(s) 1153
Ksp22I TGATCA 3 cut(s) 820, 1249, 1362
KspI CCGCGG 1 cut(s) 399
Kzo9I GATC 5 cut(s) 4, 492, 820, 1249, 1362
LguI GCTCTTC 1 cut(s) 596
LmnI GCTCC 3 cut(s) 640, 652, 1690
Lsp1109I GCAGC 4 cut(s) 168, 805, 1448, 1660
LweI GCATC 4 cut(s) 273, 793, 1589, 1690
MaeI CTAG 4 cut(s) 299, 813, 1476, 1744
MaeII ACGT 3 cut(s) 82, 411, 1280
MaeIII GTNAC 3 cut(s) 401, 863, 1306
MalI GATC 5 cut(s) 6, 494, 822, 1251, 1364
MboI GATC 5 cut(s) 4, 492, 820, 1249, 1362
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 4 cut(s) 232, 608, 632, 645
MluCI AATT 3 cut(s) 1420, 1426, 1549
MmeI TCCRAC 2 cut(s) 48, 912
Mph1103I ATGCAT 1 cut(s) 1392
MroI TCCGGA 1 cut(s) 1153
MseI TTAA 2 cut(s) 1149, 1731
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 6 cut(s) 65, 551, 569, 615, 635, 1154
MspR9I CCNGG 4 cut(s) 551, 610, 616, 784
Mva1269I GAATGC 3 cut(s) 886, 997, 1390
MvaI CCWGG 2 cut(s) 610, 784
MvnI CGCG 4 cut(s) 107, 146, 398, 668
MwoI GCNNNNNNNGC 8 cut(s) 484, 569, 578, 640, 1387, 1396, 1535, 1722
NciI CCSGG 2 cut(s) 551, 616
NdeI CATATG 1 cut(s) 1386
NdeII GATC 5 cut(s) 4, 492, 820, 1249, 1362
NlaIII CATG 5 cut(s) 130, 982, 994, 1523, 1655
NlaIV GGNNCC 6 cut(s) 230, 231, 244, 549, 630, 1131
NmeAIII GCCGAG 2 cut(s) 562, 902
NmuCI GTSAC 1 cut(s) 863
NruI TCGCGA 1 cut(s) 146
NsbI TGCGCA 2 cut(s) 287, 792
NsiI ATGCAT 1 cut(s) 1392
NspI RCATGY 1 cut(s) 1523
NspV TTCGAA 1 cut(s) 1424
PagI TCATGA 1 cut(s) 1651
PaqCI CACCTGC 1 cut(s) 1090
PasI CCCWGGG 1 cut(s) 609
PciI ACATGT 1 cut(s) 1519
PciSI GCTCTTC 1 cut(s) 596
PcsI WCGNNNNNNNCGW 3 cut(s) 138, 321, 534
PctI GAATGC 3 cut(s) 886, 997, 1390
PfeI GAWTC 5 cut(s) 68, 151, 565, 1351, 1459
PfoI TCCNGGA 1 cut(s) 782
PkrI GCNGC 9 cut(s) 106, 158, 319, 379, 707, 710, 795, 1438, 1675
PpuMI RGGWCCY 1 cut(s) 780
PscI ACATGT 1 cut(s) 1519
Psp124BI GAGCTC 1 cut(s) 645
Psp5II RGGWCCY 1 cut(s) 780
Psp6I CCWGG 2 cut(s) 608, 782
PspFI CCCAGC 6 cut(s) 46, 186, 468, 1184, 1529, 1716
PspGI CCWGG 2 cut(s) 608, 782
PspN4I GGNNCC 6 cut(s) 230, 231, 244, 549, 630, 1131
PspOMI GGGCCC 2 cut(s) 228, 628
PspPI GGNCC 8 cut(s) 228, 229, 242, 547, 612, 628, 629, 780
PspPPI RGGWCCY 1 cut(s) 780
PsrI GAACNNNNNNTAC 2 cut(s) 356, 388
PsuI RGATCY 1 cut(s) 4
RruI TCGCGA 1 cut(s) 146
RsaI GTAC 3 cut(s) 350, 458, 665
RsaNI GTAC 3 cut(s) 349, 457, 664
SacI GAGCTC 1 cut(s) 645
SacII CCGCGG 1 cut(s) 399
SalI GTCGAC 2 cut(s) 116, 197
SapI GCTCTTC 1 cut(s) 596
SaqAI TTAA 2 cut(s) 1149, 1731
SatI GCNGC 9 cut(s) 105, 157, 318, 378, 706, 709, 794, 1437, 1674
Sau3AI GATC 5 cut(s) 4, 492, 820, 1249, 1362
Sau96I GGNCC 8 cut(s) 228, 229, 242, 547, 612, 628, 629, 780
ScrFI CCNGG 4 cut(s) 551, 610, 616, 784
SduI GDGCHC 4 cut(s) 232, 608, 632, 645
SfaNI GCATC 4 cut(s) 273, 793, 1589, 1690
SfcI CTRYAG 1 cut(s) 1468
Sfr303I CCGCGG 1 cut(s) 399
SfuI TTCGAA 1 cut(s) 1424
SgrAI CRCCGGYG 1 cut(s) 634
SgrBI CCGCGG 1 cut(s) 399
SinI GGWCC 2 cut(s) 547, 780
Sse9I AATT 3 cut(s) 1420, 1426, 1549
SspI AATATT 1 cut(s) 1736
SspMI CTAG 4 cut(s) 299, 813, 1476, 1744
SstI GAGCTC 1 cut(s) 645
StyD4I CCNGG 4 cut(s) 549, 608, 614, 782
StyI CCWWGG 5 cut(s) 261, 277, 798, 807, 1686
TaaI ACNGT 2 cut(s) 142, 827
TaiI ACGT 3 cut(s) 85, 414, 1283
TasI AATT 3 cut(s) 1420, 1426, 1549
TatI WGTACW 2 cut(s) 348, 456
TauI GCSGC 5 cut(s) 107, 320, 380, 708, 711
TfiI GAWTC 5 cut(s) 68, 151, 565, 1351, 1459
Tru1I TTAA 2 cut(s) 1149, 1731
Tru9I TTAA 2 cut(s) 1149, 1731
TscAI CASTG 3 cut(s) 830, 1217, 1321
TseFI GTSAC 1 cut(s) 863
TseI GCWGC 4 cut(s) 156, 793, 1436, 1673
Tsp45I GTSAC 1 cut(s) 863
TspDTI ATGAA 3 cut(s) 322, 605, 1235
TspGWI ACGGA 5 cut(s) 537, 662, 765, 1103, 1578
TspRI CASTG 3 cut(s) 830, 1217, 1321
VpaK11BI GGWCC 2 cut(s) 547, 780
XapI RAATTY 1 cut(s) 1549
XceI RCATGY 1 cut(s) 1523
XmiI GTMKAC 2 cut(s) 117, 198
XspI CTAG 4 cut(s) 299, 813, 1476, 1744
Zsp2I ATGCAT 1 cut(s) 1392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.