Rh5BG360800

pre-mRNA-processing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0004435
Physical Location & Seq
Reverse (-)
56389555 .. 56390418
864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG360800.1

Sequence Viewer

Length: 864 bp
ATGGGAGAGGCTATACTCGTCGAAAGAACTTGTTTTCATGGAAAAGAAGAGAGGGATTATCAGGGAAGGTCTTGGATAGCGCCGCCTAGTTACGCCAAGGCCACCAATGAGTACTGTTTTACACCCAAGAGGCTGGTCCACACTTGGAGTGGCCACACAAAGGGTGTGTCTGCTATTAGGTTCTACCCCAGATACGGGCATTTACTTCTCTCTGCAGGGATGGATAGTAGGGTCAAGATCTGGGACGTTTTCAATTACGGGAACTGCATGAGAACATACATGGGTCATTCCGAGGCAGTTAGGGACATTTGTTTCTCTAATGATGGCACTAAGTTTTTAAGCTCTTCTTTTGATAAGAATATCAAGTACTGGGATACAGAAACTGGCCAAGTCATATCCACCTTTTCGACCGGAAAAGTACCGTATGTGATTAAGCTTAATCCGGATGAGGATAAGCAGAATGTGTTATTGGCGGGGATGAGTGACAATAAGATTGTTCAGTGGGATGTGAACGAGGGGAAGAAGATCACTCAAAAGTATGATCAGCATTTGCGTGCCGTGGATACCATTACGTTTGTTGATAATAACAGGAGGTTTGTTACTTCAAGTGATGACAAGTCTCTGCGTGTCTGGGAGTTTGGGATTCCTGTGGTGGTCAAGTGCATCAGTGAGCCTCACATGTATTCTATGCCTTCGGTTTCCTTGCACCCTAGTTCGAATTGGCTTGCGGCACAGAGCATGGATAACCAGATTCTTGTTTACAGCACTGGGGAGAAATTTCAGTTAGAAACCAGGGCATGTTGTTGCAGGGCATGCTTGCCAAGTGAATTTCTCGCCAGATGGACGGTTTGTTTTCTCTGGTGA

Protein Analysis

287

Amino Acids

32.96

Weight (kDa)

8.26

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 43 - 138 1.7e-18 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 43 - 139 2.2e-12 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 43 - 183 5.8e-26 WDR5 beta-propeller domain
WD40 PF00400 44 - 82 1e-07 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 45 - 211 1.2e-17 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 46 - 136 4.7e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3 PF25174 47 - 262 3.6e-29 THOC3 beta-propeller domain
WD40_Gbeta PF25391 48 - 118 3.4e-06 G protein beta WD-40 repeat protein
EIF3I PF24805 50 - 150 1.2e-13 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 50 - 138 2.1e-06 CAF1B/HIR1 beta-propeller domain
WDR55 PF24796 55 - 258 4.1e-18 WDR55
Beta-prop_WDR3_2nd PF25172 65 - 136 2.1e-11 WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 69 - 124 4e-06 MABP1/WDR62 second WD40 domain
Beta-prop_EML PF23409 75 - 256 2.6e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 87 - 125 9.6e-10 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 88 - 151 1.6e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 91 - 214 6.3e-10 TEP-1 second beta-propeller
Beta-prop_WDR41 PF25178 94 - 219 1.7e-06 WDR41
WD40_RFWD3 PF23419 96 - 178 1.6e-08 E3 ubiquitin-protein ligase RFWD3 WD40 domain
WD40_Gbeta PF25391 109 - 244 3.3e-11 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_1st PF25171 127 - 213 8.8e-10 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 143 - 258 5.6e-14 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 146 - 255 3.2e-11 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 151 - 257 9.3e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 153 - 249 3.1e-07 WDR36/Utp21 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 442
AciI CCGC 3 cut(s) 83, 473, 728
AcoI YGGCCR 2 cut(s) 151, 385
AcsI RAATTY 2 cut(s) 776, 827
AfaI GTAC 3 cut(s) 113, 368, 420
AfiI CCNNNNNNNGG 3 cut(s) 160, 194, 195
AflIII ACRYGT 1 cut(s) 678
AgsI TTSAA 2 cut(s) 253, 606
AjnI CCWGG 1 cut(s) 791
AjuI GAANNNNNNNTTGG 2 cut(s) 452, 484
AluBI AGCT 2 cut(s) 342, 436
AluI AGCT 2 cut(s) 342, 436
Alw26I GTCTC 1 cut(s) 624
AlwNI CAGNNNCTG 1 cut(s) 383
Aor13HI TCCGGA 1 cut(s) 442
AoxI GGCC 3 cut(s) 99, 151, 385
ApoI RAATTY 2 cut(s) 776, 827
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
AspLEI GCGC 1 cut(s) 82
AspS9I GGNCC 1 cut(s) 136
AsuII TTCGAA 1 cut(s) 716
AvaII GGWCC 1 cut(s) 136
BalI TGGCCA 2 cut(s) 153, 387
BccI CCATC 3 cut(s) 214, 317, 834
BceAI ACGGC 1 cut(s) 542
BciT130I CCWGG 1 cut(s) 793
BciVI GTATCC 2 cut(s) 367, 556
BclI TGATCA 1 cut(s) 541
BcoDI GTCTC 1 cut(s) 624
BfaI CTAG 2 cut(s) 87, 711
BfmI CTRYAG 1 cut(s) 213
BfoI RGCGCY 1 cut(s) 83
BfuI GTATCC 2 cut(s) 367, 556
BglII AGATCT 1 cut(s) 237
BisI GCNGC 2 cut(s) 83, 729
BlsI GCNGC 2 cut(s) 84, 730
BmcAI AGTACT 2 cut(s) 113, 368
Bme1390I CCNGG 1 cut(s) 793
Bme18I GGWCC 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 136
BmrFI CCNGG 1 cut(s) 793
BmrI ACTGGG 2 cut(s) 379, 777
BmsI GCATC 1 cut(s) 672
BmuI ACTGGG 2 cut(s) 379, 777
Bpu14I TTCGAA 1 cut(s) 716
BsaJI CCNNGG 4 cut(s) 96, 291, 558, 792
BsaWI WCCGGW 2 cut(s) 410, 442
Bsc4I CCNNNNNNNGG 3 cut(s) 160, 194, 195
Bse1I ACTGG 3 cut(s) 374, 388, 772
BseAI TCCGGA 1 cut(s) 442
BseBI CCWGG 1 cut(s) 793
BseDI CCNNGG 4 cut(s) 96, 291, 558, 792
BseGI GGATG 4 cut(s) 225, 451, 483, 511
BseLI CCNNNNNNNGG 3 cut(s) 160, 194, 195
BseNI ACTGG 3 cut(s) 374, 388, 772
Bsh1285I CGRYCG 1 cut(s) 411
BshFI GGCC 3 cut(s) 101, 153, 387
BsiEI CGRYCG 1 cut(s) 411
BsiSI CCGG 2 cut(s) 411, 443
BslFI GGGAC 2 cut(s) 257, 317
BslI CCNNNNNNNGG 3 cut(s) 160, 194, 195
BsmAI GTCTC 1 cut(s) 624
BsmFI GGGAC 2 cut(s) 257, 317
BsnI GGCC 3 cut(s) 101, 153, 387
Bsp119I TTCGAA 1 cut(s) 716
Bsp13I TCCGGA 1 cut(s) 442
Bsp143I GATC 3 cut(s) 237, 525, 541
BspACI CCGC 3 cut(s) 83, 473, 728
BspANI GGCC 3 cut(s) 101, 153, 387
BspEI TCCGGA 1 cut(s) 442
BspMAI CTGCAG 1 cut(s) 217
BspQI GCTCTTC 1 cut(s) 349
BspT104I TTCGAA 1 cut(s) 716
BsrI ACTGG 3 cut(s) 374, 388, 772
BssECI CCNNGG 4 cut(s) 96, 291, 558, 792
BssMI GATC 3 cut(s) 237, 525, 541
BssT1I CCWWGG 1 cut(s) 96
Bst2UI CCWGG 1 cut(s) 793
Bst4CI ACNGT 3 cut(s) 116, 423, 847
Bst6I CTCTTC 2 cut(s) 42, 349
BstAPI GCANNNNNTGC 1 cut(s) 813
BstBI TTCGAA 1 cut(s) 716
BstC8I GCNNGC 4 cut(s) 555, 726, 814, 818
BstDEI CTNAG 1 cut(s) 330
BstDSI CCRYGG 1 cut(s) 558
BstF5I GGATG 4 cut(s) 225, 451, 483, 511
BstH2I RGCGCY 1 cut(s) 83
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 3 cut(s) 240, 528, 544
BstMAI GTCTC 1 cut(s) 624
BstMBI GATC 3 cut(s) 237, 525, 541
BstMCI CGRYCG 1 cut(s) 411
BstMWI GCNNNNNNNGC 1 cut(s) 813
BstNI CCWGG 1 cut(s) 793
BstNSI RCATGY 3 cut(s) 682, 801, 816
BstSCI CCNGG 1 cut(s) 791
BstSFI CTRYAG 1 cut(s) 213
BstX2I RGATCY 1 cut(s) 237
BstXI CCANNNNNNTGG 1 cut(s) 133
BstYI RGATCY 1 cut(s) 237
BsuI GTATCC 2 cut(s) 367, 556
BsuRI GGCC 3 cut(s) 101, 153, 387
BtgI CCRYGG 1 cut(s) 558
BtsCI GGATG 4 cut(s) 225, 451, 483, 511
BtsIMutI CAGTG 3 cut(s) 506, 673, 765
Cac8I GCNNGC 4 cut(s) 555, 726, 814, 818
CaiI CAGNNNCTG 1 cut(s) 383
CfoI GCGC 1 cut(s) 82
Cfr13I GGNCC 1 cut(s) 136
Csp6I GTAC 3 cut(s) 112, 367, 419
CviAII CATG 7 cut(s) 38, 268, 280, 679, 739, 798, 813
CviJI RGCY 9 cut(s) 11, 101, 133, 153, 342, 387, 436, 673, 724
CviKI_1 RGCY 9 cut(s) 11, 101, 133, 153, 342, 387, 436, 673, 724
CviQI GTAC 3 cut(s) 112, 367, 419
DdeI CTNAG 1 cut(s) 330
DpnI GATC 3 cut(s) 239, 527, 543
DpnII GATC 3 cut(s) 237, 525, 541
EaeI YGGCCR 2 cut(s) 151, 385
Eam1104I CTCTTC 2 cut(s) 42, 349
EarI CTCTTC 2 cut(s) 42, 349
Eco130I CCWWGG 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 136
EcoRII CCWGG 1 cut(s) 791
EcoT14I CCWWGG 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 96
FaeI CATG 7 cut(s) 41, 271, 283, 682, 742, 801, 816
FalI AAGNNNNNCTT 2 cut(s) 331, 363
FaqI GGGAC 2 cut(s) 257, 317
FatI CATG 7 cut(s) 37, 267, 279, 678, 738, 797, 812
FauI CCCGC 1 cut(s) 466
FbaI TGATCA 1 cut(s) 541
Fnu4HI GCNGC 2 cut(s) 83, 729
FokI GGATG 4 cut(s) 232, 458, 490, 518
Fsp4HI GCNGC 2 cut(s) 83, 729
FspBI CTAG 2 cut(s) 87, 711
GlaI GCGC 1 cut(s) 81
GluI GCNGC 2 cut(s) 83, 729
HaeII RGCGCY 1 cut(s) 83
HaeIII GGCC 3 cut(s) 101, 153, 387
HapII CCGG 2 cut(s) 411, 443
HhaI GCGC 1 cut(s) 82
Hin1II CATG 7 cut(s) 41, 271, 283, 682, 742, 801, 816
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HindIII AAGCTT 1 cut(s) 434
HinfI GANTC 2 cut(s) 643, 751
HpaII CCGG 2 cut(s) 411, 443
Hpy166II GTNNAC 3 cut(s) 139, 511, 760
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 2 cut(s) 235, 443
Hpy8I GTNNAC 3 cut(s) 139, 511, 760
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 2 cut(s) 60, 702
HpyCH4III ACNGT 3 cut(s) 116, 423, 847
HpyCH4IV ACGT 2 cut(s) 246, 572
HpyCH4V TGCA 5 cut(s) 215, 267, 663, 706, 807
HpyF10VI GCNNNNNNNGC 1 cut(s) 813
HpyF3I CTNAG 1 cut(s) 330
HpySE526I ACGT 2 cut(s) 246, 572
Hsp92II CATG 7 cut(s) 41, 271, 283, 682, 742, 801, 816
HspAI GCGC 1 cut(s) 80
Kpn2I TCCGGA 1 cut(s) 442
Ksp22I TGATCA 1 cut(s) 541
Kzo9I GATC 3 cut(s) 237, 525, 541
LguI GCTCTTC 1 cut(s) 349
LweI GCATC 1 cut(s) 672
MaeI CTAG 2 cut(s) 87, 711
MaeII ACGT 2 cut(s) 246, 572
MaeIII GTNAC 3 cut(s) 89, 482, 598
MalI GATC 3 cut(s) 239, 527, 543
MboI GATC 3 cut(s) 237, 525, 541
MboII GAAGA 4 cut(s) 59, 336, 532, 535
MflI RGATCY 1 cut(s) 237
MlsI TGGCCA 2 cut(s) 153, 387
MluCI AATT 4 cut(s) 253, 718, 776, 827
MluNI TGGCCA 2 cut(s) 153, 387
MnlI CCTC 7 cut(s) 45, 123, 286, 442, 508, 585, 684
Mox20I TGGCCA 2 cut(s) 153, 387
MroI TCCGGA 1 cut(s) 442
MscI TGGCCA 2 cut(s) 153, 387
MseI TTAA 3 cut(s) 338, 432, 438
MslI CAYNNNNRTG 1 cut(s) 552
Msp20I TGGCCA 2 cut(s) 153, 387
MspI CCGG 2 cut(s) 411, 443
MspR9I CCNGG 1 cut(s) 793
MvaI CCWGG 1 cut(s) 793
MwoI GCNNNNNNNGC 1 cut(s) 813
NdeII GATC 3 cut(s) 237, 525, 541
NlaIII CATG 7 cut(s) 41, 271, 283, 682, 742, 801, 816
NmuCI GTSAC 1 cut(s) 482
NspI RCATGY 3 cut(s) 682, 801, 816
NspV TTCGAA 1 cut(s) 716
PaeI GCATGC 1 cut(s) 816
PciI ACATGT 1 cut(s) 678
PciSI GCTCTTC 1 cut(s) 349
PfeI GAWTC 2 cut(s) 643, 751
PkrI GCNGC 2 cut(s) 84, 730
PscI ACATGT 1 cut(s) 678
Psp6I CCWGG 1 cut(s) 791
PspGI CCWGG 1 cut(s) 791
PspPI GGNCC 1 cut(s) 136
PstI CTGCAG 1 cut(s) 217
PstNI CAGNNNCTG 1 cut(s) 383
PsuI RGATCY 1 cut(s) 237
RsaI GTAC 3 cut(s) 113, 368, 420
RsaNI GTAC 3 cut(s) 112, 367, 419
RseI CAYNNNNRTG 1 cut(s) 552
SapI GCTCTTC 1 cut(s) 349
SaqAI TTAA 3 cut(s) 338, 432, 438
SatI GCNGC 2 cut(s) 83, 729
Sau3AI GATC 3 cut(s) 237, 525, 541
Sau96I GGNCC 1 cut(s) 136
ScaI AGTACT 2 cut(s) 113, 368
ScrFI CCNGG 1 cut(s) 793
SetI ASST 8 cut(s) 71, 182, 249, 344, 404, 438, 575, 596
SfaNI GCATC 1 cut(s) 672
SfcI CTRYAG 1 cut(s) 213
SfuI TTCGAA 1 cut(s) 716
SinI GGWCC 1 cut(s) 136
SmiMI CAYNNNNRTG 1 cut(s) 552
SphI GCATGC 1 cut(s) 816
Sse9I AATT 4 cut(s) 253, 718, 776, 827
SsiI CCGC 3 cut(s) 83, 473, 728
SspMI CTAG 2 cut(s) 87, 711
StyD4I CCNGG 1 cut(s) 791
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 3 cut(s) 116, 423, 847
TaiI ACGT 2 cut(s) 249, 575
TaqI TCGA 3 cut(s) 21, 407, 716
TasI AATT 4 cut(s) 253, 718, 776, 827
TatI WGTACW 2 cut(s) 111, 366
TauI GCSGC 2 cut(s) 85, 731
TfiI GAWTC 2 cut(s) 643, 751
Tru1I TTAA 3 cut(s) 338, 432, 438
Tru9I TTAA 3 cut(s) 338, 432, 438
TscAI CASTG 3 cut(s) 506, 673, 772
TseFI GTSAC 1 cut(s) 482
Tsp45I GTSAC 1 cut(s) 482
TspDTI ATGAA 1 cut(s) 26
TspRI CASTG 3 cut(s) 506, 673, 772
VpaK11BI GGWCC 1 cut(s) 136
XapI RAATTY 2 cut(s) 776, 827
XceI RCATGY 3 cut(s) 682, 801, 816
XcmI CCANNNNNNNNNTGG 1 cut(s) 146
XspI CTAG 2 cut(s) 87, 711
ZrmI AGTACT 2 cut(s) 113, 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.