MD13G1091700.v1.1

pre-mRNA-processing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004435
Physical Location & Seq
Reverse (-)
6466003 .. 6469517
3515 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1091700.v1.1.491

Sequence Viewer

Length: 1779 bp
ATGGATCTTCTCCGAGCATACTCTGATCAAAACGACGACGAAACGAACGAGCCAGAGGAGCACAACCAAAACCCTAAATCCGCACCGTCCTCCCCAGACGCGTCGCCGCCTCGGCTCCTCCCCGCCAAGTCCGCGGCGCCCAATGTCGACGACACTATGCTGGCCCTGGCGGTAGCCGGGGCCAACCGGTCTTCCACCCGCCCCATCGACCCGACCCAACACATCGTCGGCTTCAACCCCACGTACGACCAGCTATGGGCGCCCATCTACGGCCCATCCCACCCGTACGCCAAGGACGGCATCGCCCAGGGGATGCGCAACCACAAGCTCGGCTTCGTGGAGGACGCATCGATTGAGCCCTTCGTGTTCGACGAGCAGTACAATACTTTTCACAAGTATGGGTATGCTGCTGATCCATCGGCCTCTGCTGGGTACAATTATGTTGGAGATTTCGAGGCCTTGCAGAAGAACGACGCCGTCTCTGTGTATAACATTCCCCAGCACGAGCAGAAGAAGCGGAAGATTGAAAAGAGGAAGGAATTGGAGGAGAATGAGGGTGAGGGTGAGGATGAAGATATGGACCCCGAGGAGGTCCAGAACCCGGCTAGTGACGTGTGGTTGATGAAGAATAAGAAGAGTCCGTGGGCGGGAAAGAAGGAGGGTCTGCCAACGGAGCTGACTGAGGAGCAGAAGAAGTATGCTGAGGAGTATGCCAAGAAGAAAGGCGAAGAGAAAGGGGCTGGCGATAAGGGTGAGGCTGTGGTTGAGAAGACTACGTTTCATGGGAAGGAAGAGAGGGATTATCAGGGTAGGTCTTGGATTGCACCGCCGAGAGATGCTAAGGCGTCTAATGATCACTGTTATATTCCAAAGAGGTTGGTGCACACTTGGAGCGGGCACACGAAGGGTGTGTCTGCTATTAGGTTCTTCCCTAAGTACGGGCATTTGATTCTCTCTGCCGGGATGGATACCAAGGTGAAGATTTGGGATGTTTTCAATACCGGAAAGTGTATGAGAACTTACATGGGTCACTCTAAGGCAGTTCGGGATATTTGTTTCTCTAATGATGGGAGTAGGTTTTTGACTGCTAGTTATGATAAGAATATCAAGTACTGGGATACTGAAACTGGGCAGGTGATATCTACATTCTCAACCGGGAAGGTTCCTTATGTTGTCAAGCTGAATCCAGACGAGGATAAGCAGAATGTATTGTTGGCAGGTATGAGTGATAAGAAGATTGTTCAGTGGGATATGAATGAGGGGAAGATAAATCAAGAGTATGATCAGCATTTGGGTGCTGTGAATACGATTACATTTGTGGATAATAACCGTAGGTTTGTTACTTCCAGCGATGACAAGTCGCTTCGCGTATGGGAGTTTGGGATCCCTGTGGTTATTAAGTATATTAGTGAGCCTCATATGCATTCTATGCCATCAATTTCACTGCACCCCAATTCCAATTGGCTTGCAGCACAGAGTATGGACAATCAGATTCTGATTTATAGCACTAGGGAGAAGTTTCAGCTCAATAAGAAGAAGAGGTTTGCCGGGCATGTTGTCGCCGGGTATGCTTGCCAAGTCAATTTCTCGCCAGACGGACGGTTTGTTATGTCGGGTGATGGTGAAGGTAGATGCTGGTTTTGGGATTGGAAGACATGCAAAGTCTTCAGAACTCTCAAATGTCATGAAGGGGTATGCATTGGGGCTGCATGGCATCCCTTGGAGCAAAGTAAAGTTGCAACATGCGGCTGGGATGGCTTGATTAAGTACTGGGACTAG

Protein Analysis

593

Amino Acids

67.04

Weight (kDa)

6.13

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40 PF00400 292 - 330 9.6e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 293 - 398 2.3e-18 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 293 - 386 1.5e-17 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 293 - 459 6.8e-10 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 293 - 458 3.9e-16 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 294 - 450 1.1e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EML_2 PF23414 294 - 383 1.3e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 296 - 368 6.3e-07 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 297 - 591 3.4e-53 WDR5 beta-propeller domain
EIF3I PF24805 298 - 399 3.6e-13 EIF3I
WD40_Prp19 PF24814 300 - 384 1.7e-12 Prp19 WD40 domain
WDR55 PF24796 303 - 460 1.9e-17 WDR55
Beta-prop_WDR3_2nd PF25172 312 - 383 3.3e-10 WDR3 second beta-propeller domain
Beta-prop_ACSF4 PF13570 333 - 562 9.7e-06 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 333 - 399 5.2e-11 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 335 - 373 2.2e-10 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 338 - 459 3.4e-10 TEP-1 second beta-propeller
Beta-prop_WDR41 PF25178 344 - 464 5e-06 WDR41
WD40_RFWD3 PF23419 344 - 419 7.6e-08 E3 ubiquitin-protein ligase RFWD3 WD40 domain
WD40_Gbeta PF25391 357 - 460 1.4e-11 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 359 - 459 9.2e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 359 - 456 3.3e-06 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 375 - 460 9.7e-09 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 390 - 566 5.1e-23 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 399 - 502 1.6e-11 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 400 - 586 2e-15 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 401 - 503 9.5e-07 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 402 - 508 4.1e-07 WDR36/Utp21 second beta-propeller domain
EIF3I PF24805 403 - 564 5.7e-08 EIF3I
Beta-prop_THOC3 PF25174 403 - 592 5.8e-23 THOC3 beta-propeller domain
WDR90_beta-prop_4th PF23342 434 - 568 1.1e-06 WDR90, 4th beta-propeller
WD40_CDC20-Fz PF24807 471 - 592 8.2e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 473 - 592 2.3e-09 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 475 - 556 2.2e-08 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 478 - 592 4.6e-08 WDR55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1123
Acc16I TGCGCA 1 cut(s) 317
Acc36I ACCTGC 2 cut(s) 1123, 1208
AccB1I GGYRCC 2 cut(s) 136, 259
AccBSI CCGCTC 1 cut(s) 894
AccI GTMKAC 1 cut(s) 147
AccII CGCG 3 cut(s) 101, 134, 1368
AclWI GGATC 4 cut(s) 12, 407, 1378, 1391
AcuI CTGAAG 1 cut(s) 1651
AcyI GRCGYC 4 cut(s) 137, 260, 474, 845
AfaI GTAC 7 cut(s) 245, 287, 380, 434, 938, 1112, 1769
AfiI CCNNNNNNNGG 7 cut(s) 256, 269, 429, 589, 601, 647, 938
AflIII ACRYGT 2 cut(s) 99, 612
AgeI ACCGGT 1 cut(s) 186
AgsI TTSAA 3 cut(s) 235, 527, 997
AjiI CACGTC 1 cut(s) 613
AjnI CCWGG 2 cut(s) 165, 306
AjuI GAANNNNNNNTTGG 2 cut(s) 1196, 1228
AluBI AGCT 5 cut(s) 253, 328, 676, 1180, 1525
AluI AGCT 5 cut(s) 253, 328, 676, 1180, 1525
Alw21I GWGCWC 2 cut(s) 63, 885
Alw26I GTCTC 1 cut(s) 484
Alw44I GTGCAC 1 cut(s) 881
AlwI GGATC 4 cut(s) 12, 407, 1378, 1391
AlwNI CAGNNNCTG 1 cut(s) 1495
Ama87I CYCGRG 1 cut(s) 584
AoxI GGCC 5 cut(s) 162, 180, 271, 420, 456
ApaLI GTGCAC 1 cut(s) 881
ApeKI GCWGC 3 cut(s) 407, 1469, 1706
ArsI GACNNNNNNTTYG 4 cut(s) 896, 928, 1586, 1618
AsiGI ACCGGT 1 cut(s) 186
AspLEI GCGC 3 cut(s) 139, 262, 318
AspS9I GGNCC 5 cut(s) 163, 180, 272, 580, 592
AsuC2I CCSGG 6 cut(s) 178, 602, 961, 1156, 1549, 1564
AsuHPI GGTGA 7 cut(s) 569, 575, 764, 988, 1147, 1628, 1634
AvaI CYCGRG 1 cut(s) 584
AvaII GGWCC 2 cut(s) 580, 592
BaeGI GKGCMC 2 cut(s) 885, 900
BaeI ACNNNNGTAYC 2 cut(s) 960, 993
BamHI GGATCC 1 cut(s) 1383
BanI GGYRCC 2 cut(s) 136, 259
BanII GRGCYC 1 cut(s) 360
BauI CACGAG 1 cut(s) 503
BbsI GAAGAC 4 cut(s) 183, 776, 1657, 1658
Bbv12I GWGCWC 2 cut(s) 63, 885
BbvCI CCTCAGC 1 cut(s) 702
BbvI GCAGC 3 cut(s) 394, 1481, 1693
BccI CCATC 9 cut(s) 212, 272, 283, 424, 958, 1061, 1441, 1613, 1748
BceAI ACGGC 3 cut(s) 286, 313, 461
BciT130I CCWGG 2 cut(s) 167, 308
BciVI GTATCC 2 cut(s) 961, 1111
BclI TGATCA 3 cut(s) 25, 853, 1282
BcnI CCSGG 6 cut(s) 178, 602, 961, 1156, 1549, 1564
BcoDI GTCTC 1 cut(s) 484
BfaI CTAG 4 cut(s) 606, 1089, 1509, 1777
BfoI RGCGCY 2 cut(s) 140, 263
BfuAI ACCTGC 2 cut(s) 1123, 1208
BfuI GTATCC 2 cut(s) 961, 1111
BglI GCCNNNNNGGC 1 cut(s) 112
BisI GCNGC 6 cut(s) 107, 135, 408, 1470, 1707, 1747
BlsI GCNGC 6 cut(s) 108, 136, 409, 1471, 1708, 1748
BmcAI AGTACT 2 cut(s) 1112, 1769
Bme1390I CCNGG 8 cut(s) 167, 178, 308, 602, 961, 1156, 1549, 1564
Bme18I GGWCC 2 cut(s) 580, 592
BmeT110I CYCGRG 1 cut(s) 584
BmgBI CACGTC 1 cut(s) 613
BmgT120I GGNCC 5 cut(s) 163, 180, 272, 580, 592
BmiI GGNNCC 7 cut(s) 116, 138, 181, 261, 582, 1164, 1385
BmrFI CCNGG 8 cut(s) 167, 178, 308, 602, 961, 1156, 1549, 1564
BmrI ACTGGG 2 cut(s) 1123, 1137
BmsI GCATC 6 cut(s) 303, 309, 356, 826, 1622, 1723
BmuI ACTGGG 2 cut(s) 1123, 1137
BpiI GAAGAC 4 cut(s) 183, 776, 1657, 1658
Bpu10I CCTNAGC 2 cut(s) 702, 840
BpuMI CCSGG 6 cut(s) 178, 602, 961, 1156, 1549, 1564
Bsa29I ATCGAT 1 cut(s) 350
BsaAI YACGTR 1 cut(s) 243
BsaHI GRCGYC 4 cut(s) 137, 260, 474, 845
BsaWI WCCGGW 2 cut(s) 186, 1001
Bsc4I CCNNNNNNNGG 7 cut(s) 256, 269, 429, 589, 601, 647, 938
Bse118I RCCGGY 1 cut(s) 186
Bse1I ACTGG 3 cut(s) 1118, 1132, 1775
BseBI CCWGG 2 cut(s) 167, 308
BseCI ATCGAT 1 cut(s) 350
BseGI GGATG 7 cut(s) 275, 318, 574, 969, 994, 1714, 1759
BseLI CCNNNNNNNGG 7 cut(s) 256, 269, 429, 589, 601, 647, 938
BseMII CTCAG 2 cut(s) 672, 693
BseNI ACTGG 3 cut(s) 1118, 1132, 1775
BseRI GAGGAG 6 cut(s) 71, 107, 560, 602, 698, 719
BseSI GKGCMC 2 cut(s) 885, 900
BseXI GCAGC 3 cut(s) 394, 1481, 1693
BseYI CCCAGC 3 cut(s) 428, 498, 1749
BsgI GTGCAG 1 cut(s) 1430
Bsh1236I CGCG 3 cut(s) 101, 134, 1368
BshFI GGCC 5 cut(s) 164, 182, 273, 422, 458
BshNI GGYRCC 2 cut(s) 136, 259
BshTI ACCGGT 1 cut(s) 186
BshVI ATCGAT 1 cut(s) 350
BsiHKAI GWGCWC 2 cut(s) 63, 885
BsiHKCI CYCGRG 1 cut(s) 584
BsiSI CCGG 8 cut(s) 177, 187, 602, 960, 1002, 1155, 1548, 1563
BsiWI CGTACG 2 cut(s) 243, 285
BslI CCNNNNNNNGG 7 cut(s) 256, 269, 429, 589, 601, 647, 938
BsmAI GTCTC 1 cut(s) 484
BsmBI CGTCTC 1 cut(s) 484
BsmI GAATGC 1 cut(s) 1423
BsnI GGCC 5 cut(s) 164, 182, 273, 422, 458
BsoBI CYCGRG 1 cut(s) 584
Bsp1286I GDGCHC 4 cut(s) 63, 360, 885, 900
Bsp143I GATC 6 cut(s) 4, 25, 412, 853, 1282, 1383
BspANI GGCC 5 cut(s) 164, 182, 273, 422, 458
BspCNI CTCAG 2 cut(s) 673, 694
BspDI ATCGAT 1 cut(s) 350
BspFNI CGCG 3 cut(s) 101, 134, 1368
BspHI TCATGA 1 cut(s) 1684
BspLI GGNNCC 7 cut(s) 116, 138, 181, 261, 582, 1164, 1385
BspMI ACCTGC 2 cut(s) 1123, 1208
BspPI GGATC 4 cut(s) 12, 407, 1378, 1391
BspT107I GGYRCC 2 cut(s) 136, 259
BsrBI CCGCTC 1 cut(s) 894
BsrFI RCCGGY 1 cut(s) 186
BsrI ACTGG 3 cut(s) 1118, 1132, 1775
BssAI RCCGGY 1 cut(s) 186
BssMI GATC 6 cut(s) 4, 25, 412, 853, 1282, 1383
BssNI GRCGYC 4 cut(s) 137, 260, 474, 845
BssSI CACGAG 1 cut(s) 503
BssT1I CCWWGG 3 cut(s) 291, 972, 1719
Bst2BI CACGAG 1 cut(s) 503
Bst2UI CCWGG 2 cut(s) 167, 308
Bst4CI ACNGT 4 cut(s) 87, 860, 1331, 1602
Bst6I CTCTTC 4 cut(s) 629, 723, 786, 1532
BstACI GRCGYC 4 cut(s) 137, 260, 474, 845
BstAPI GCANNNNNTGC 1 cut(s) 1429
BstBAI YACGTR 1 cut(s) 243
BstC8I GCNNGC 5 cut(s) 162, 742, 896, 1467, 1573
BstDEI CTNAG 5 cut(s) 681, 702, 840, 933, 1035
BstDSI CCRYGG 2 cut(s) 132, 641
BstF5I GGATG 7 cut(s) 275, 318, 574, 969, 994, 1714, 1759
BstFNI CGCG 3 cut(s) 101, 134, 1368
BstH2I RGCGCY 2 cut(s) 140, 263
BstHHI GCGC 3 cut(s) 139, 262, 318
BstKTI GATC 6 cut(s) 7, 28, 415, 856, 1285, 1386
BstMAI GTCTC 1 cut(s) 484
BstMBI GATC 6 cut(s) 4, 25, 412, 853, 1282, 1383
BstNI CCWGG 2 cut(s) 167, 308
BstNSI RCATGY 3 cut(s) 1556, 1659, 1746
BstSCI CCNGG 8 cut(s) 165, 176, 306, 600, 959, 1154, 1547, 1562
BstSLI GKGCMC 2 cut(s) 885, 900
BstUI CGCG 3 cut(s) 101, 134, 1368
BstV1I GCAGC 3 cut(s) 394, 1481, 1693
BstV2I GAAGAC 4 cut(s) 183, 776, 1657, 1658
BstX2I RGATCY 2 cut(s) 4, 1383
BstYI RGATCY 2 cut(s) 4, 1383
Bsu15I ATCGAT 1 cut(s) 350
BsuI GTATCC 2 cut(s) 961, 1111
BsuRI GGCC 5 cut(s) 164, 182, 273, 422, 458
BsuTUI ATCGAT 1 cut(s) 350
BtgI CCRYGG 2 cut(s) 132, 641
BtgZI GCGATG 2 cut(s) 286, 1365
BtrI CACGTC 1 cut(s) 613
BtsCI GGATG 7 cut(s) 275, 318, 574, 969, 994, 1714, 1759
BtsI GCAGTG 1 cut(s) 1442
BtsIMutI CAGTG 3 cut(s) 856, 1250, 1442
BveI ACCTGC 2 cut(s) 1123, 1208
Cac8I GCNNGC 5 cut(s) 162, 742, 896, 1467, 1573
CaiI CAGNNNCTG 1 cut(s) 1495
CciI TCATGA 1 cut(s) 1684
CfoI GCGC 3 cut(s) 139, 262, 318
Cfr10I RCCGGY 1 cut(s) 186
Cfr13I GGNCC 5 cut(s) 163, 180, 272, 580, 592
Cfr42I CCGCGG 1 cut(s) 135
ClaI ATCGAT 1 cut(s) 350
CseI GACGC 5 cut(s) 90, 107, 353, 482, 834
Csp6I GTAC 7 cut(s) 244, 286, 379, 433, 937, 1111, 1768
CspAI ACCGGT 1 cut(s) 186
CviAII CATG 7 cut(s) 782, 1024, 1553, 1656, 1685, 1710, 1743
CviQI GTAC 7 cut(s) 244, 286, 379, 433, 937, 1111, 1768
DdeI CTNAG 5 cut(s) 681, 702, 840, 933, 1035
DinI GGCGCC 2 cut(s) 138, 261
DpnI GATC 6 cut(s) 6, 27, 414, 855, 1284, 1385
DpnII GATC 6 cut(s) 4, 25, 412, 853, 1282, 1383
Eam1104I CTCTTC 4 cut(s) 629, 723, 786, 1532
EarI CTCTTC 4 cut(s) 629, 723, 786, 1532
Eco130I CCWWGG 3 cut(s) 291, 972, 1719
Eco147I AGGCCT 1 cut(s) 458
Eco24I GRGCYC 1 cut(s) 360
Eco32I GATATC 1 cut(s) 1140
Eco47I GGWCC 2 cut(s) 580, 592
Eco57I CTGAAG 1 cut(s) 1651
Eco88I CYCGRG 1 cut(s) 584
EcoRII CCWGG 2 cut(s) 165, 306
EcoRV GATATC 1 cut(s) 1140
EcoT14I CCWWGG 3 cut(s) 291, 972, 1719
EcoT22I ATGCAT 2 cut(s) 1425, 1700
EcoT38I GRGCYC 1 cut(s) 360
EgeI GGCGCC 2 cut(s) 138, 261
EheI GGCGCC 2 cut(s) 138, 261
ErhI CCWWGG 3 cut(s) 291, 972, 1719
Esp3I CGTCTC 1 cut(s) 484
FaeI CATG 7 cut(s) 785, 1027, 1556, 1659, 1688, 1713, 1746
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FatI CATG 7 cut(s) 781, 1023, 1552, 1655, 1684, 1709, 1742
FauI CCCGC 4 cut(s) 130, 206, 640, 887
FauNDI CATATG 1 cut(s) 1419
FbaI TGATCA 3 cut(s) 25, 853, 1282
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 6 cut(s) 107, 135, 408, 1470, 1707, 1747
FokI GGATG 7 cut(s) 262, 325, 581, 976, 1001, 1701, 1766
FriOI GRGCYC 1 cut(s) 360
Fsp4HI GCNGC 6 cut(s) 107, 135, 408, 1470, 1707, 1747
FspBI CTAG 4 cut(s) 606, 1089, 1509, 1777
FspI TGCGCA 1 cut(s) 317
GlaI GCGC 3 cut(s) 138, 261, 317
GluI GCNGC 6 cut(s) 107, 135, 408, 1470, 1707, 1747
GsaI CCCAGC 3 cut(s) 432, 502, 1753
HaeII RGCGCY 2 cut(s) 140, 263
HaeIII GGCC 5 cut(s) 164, 182, 273, 422, 458
HapII CCGG 8 cut(s) 177, 187, 602, 960, 1002, 1155, 1548, 1563
HgaI GACGC 5 cut(s) 90, 107, 353, 482, 834
HhaI GCGC 3 cut(s) 139, 262, 318
Hin1I GRCGYC 4 cut(s) 137, 260, 474, 845
Hin1II CATG 7 cut(s) 785, 1027, 1556, 1659, 1688, 1713, 1746
Hin6I GCGC 3 cut(s) 137, 260, 316
HinP1I GCGC 3 cut(s) 137, 260, 316
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 4 cut(s) 637, 949, 1183, 1492
HpaII CCGG 8 cut(s) 177, 187, 602, 960, 1002, 1155, 1548, 1563
HphI GGTGA 7 cut(s) 569, 575, 764, 988, 1147, 1628, 1634
Hpy166II GTNNAC 2 cut(s) 148, 883
Hpy188I TCNGA 5 cut(s) 14, 25, 1491, 1497, 1670
Hpy188III TCNNGA 5 cut(s) 595, 1046, 1187, 1274, 1685
Hpy8I GTNNAC 2 cut(s) 148, 883
Hpy99I CGWCG 7 cut(s) 38, 41, 106, 152, 230, 374, 476
HpyAV CCTTC 8 cut(s) 370, 529, 649, 781, 898, 1153, 1619, 1682
HpyCH4III ACNGT 4 cut(s) 87, 860, 1331, 1602
HpyCH4IV ACGT 3 cut(s) 242, 612, 776
HpyF3I CTNAG 5 cut(s) 681, 702, 840, 933, 1035
HpySE526I ACGT 3 cut(s) 242, 612, 776
Hsp92I GRCGYC 4 cut(s) 137, 260, 474, 845
Hsp92II CATG 7 cut(s) 785, 1027, 1556, 1659, 1688, 1713, 1746
HspAI GCGC 3 cut(s) 137, 260, 316
KasI GGCGCC 2 cut(s) 136, 259
Ksp22I TGATCA 3 cut(s) 25, 853, 1282
KspI CCGCGG 1 cut(s) 135
Kzo9I GATC 6 cut(s) 4, 25, 412, 853, 1282, 1383
LmnI GCTCC 6 cut(s) 58, 120, 673, 685, 891, 1723
Lsp1109I GCAGC 3 cut(s) 394, 1481, 1693
LweI GCATC 6 cut(s) 303, 309, 356, 826, 1622, 1723
MaeI CTAG 4 cut(s) 606, 1089, 1509, 1777
MaeII ACGT 3 cut(s) 242, 612, 776
MaeIII GTNAC 3 cut(s) 608, 1028, 1339
MalI GATC 6 cut(s) 6, 27, 414, 855, 1284, 1385
MbiI CCGCTC 1 cut(s) 894
MboI GATC 6 cut(s) 4, 25, 412, 853, 1282, 1383
MfeI CAATTG 1 cut(s) 1459
MflI RGATCY 2 cut(s) 4, 1383
MhlI GDGCHC 4 cut(s) 63, 360, 885, 900
MluCI AATT 6 cut(s) 436, 539, 1437, 1453, 1459, 1582
MluI ACGCGT 1 cut(s) 99
Mly113I GGCGCC 2 cut(s) 137, 260
MlyI GAGTC 1 cut(s) 646
MmeI TCCRAC 1 cut(s) 424
Mph1103I ATGCAT 2 cut(s) 1425, 1700
MseI TTAA 2 cut(s) 1398, 1764
MslI CAYNNNNRTG 2 cut(s) 396, 1293
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 8 cut(s) 177, 187, 602, 960, 1002, 1155, 1548, 1563
MspR9I CCNGG 8 cut(s) 167, 178, 308, 602, 961, 1156, 1549, 1564
MunI CAATTG 1 cut(s) 1459
Mva1269I GAATGC 1 cut(s) 1423
MvaI CCWGG 2 cut(s) 167, 308
MvnI CGCG 3 cut(s) 101, 134, 1368
NarI GGCGCC 2 cut(s) 137, 260
NciI CCSGG 6 cut(s) 178, 602, 961, 1156, 1549, 1564
NdeI CATATG 1 cut(s) 1419
NdeII GATC 6 cut(s) 4, 25, 412, 853, 1282, 1383
NlaIII CATG 7 cut(s) 785, 1027, 1556, 1659, 1688, 1713, 1746
NlaIV GGNNCC 7 cut(s) 116, 138, 181, 261, 582, 1164, 1385
NmeAIII GCCGAG 3 cut(s) 91, 309, 855
NmuCI GTSAC 2 cut(s) 608, 1028
NsbI TGCGCA 1 cut(s) 317
NsiI ATGCAT 2 cut(s) 1425, 1700
NspI RCATGY 3 cut(s) 1556, 1659, 1746
PagI TCATGA 1 cut(s) 1684
PaqCI CACCTGC 1 cut(s) 1123
PasI CCCWGGG 1 cut(s) 307
PceI AGGCCT 1 cut(s) 458
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 369
PctI GAATGC 1 cut(s) 1423
PfeI GAWTC 3 cut(s) 949, 1183, 1492
Pfl23II CGTACG 2 cut(s) 243, 285
PflFI GACNNNGTC 1 cut(s) 476
PinAI ACCGGT 1 cut(s) 186
PkrI GCNGC 6 cut(s) 108, 136, 409, 1471, 1708, 1748
PleI GAGTC 1 cut(s) 645
PluTI GGCGCC 2 cut(s) 140, 263
PpsI GAGTC 1 cut(s) 645
Ppu21I YACGTR 1 cut(s) 243
Psp6I CCWGG 2 cut(s) 165, 306
PspFI CCCAGC 3 cut(s) 428, 498, 1749
PspGI CCWGG 2 cut(s) 165, 306
PspLI CGTACG 2 cut(s) 243, 285
PspN4I GGNNCC 7 cut(s) 116, 138, 181, 261, 582, 1164, 1385
PspPI GGNCC 5 cut(s) 163, 180, 272, 580, 592
PstNI CAGNNNCTG 1 cut(s) 1495
PsuI RGATCY 2 cut(s) 4, 1383
PsyI GACNNNGTC 1 cut(s) 476
RsaI GTAC 7 cut(s) 245, 287, 380, 434, 938, 1112, 1769
RsaNI GTAC 7 cut(s) 244, 286, 379, 433, 937, 1111, 1768
RseI CAYNNNNRTG 2 cut(s) 396, 1293
SacII CCGCGG 1 cut(s) 135
SalI GTCGAC 1 cut(s) 146
SaqAI TTAA 2 cut(s) 1398, 1764
SatI GCNGC 6 cut(s) 107, 135, 408, 1470, 1707, 1747
Sau3AI GATC 6 cut(s) 4, 25, 412, 853, 1282, 1383
Sau96I GGNCC 5 cut(s) 163, 180, 272, 580, 592
ScaI AGTACT 2 cut(s) 1112, 1769
SchI GAGTC 1 cut(s) 646
ScrFI CCNGG 8 cut(s) 167, 178, 308, 602, 961, 1156, 1549, 1564
SduI GDGCHC 4 cut(s) 63, 360, 885, 900
SfaNI GCATC 6 cut(s) 303, 309, 356, 826, 1622, 1723
SfoI GGCGCC 2 cut(s) 138, 261
Sfr303I CCGCGG 1 cut(s) 135
SgrBI CCGCGG 1 cut(s) 135
SinI GGWCC 2 cut(s) 580, 592
SmiMI CAYNNNNRTG 2 cut(s) 396, 1293
Sse9I AATT 6 cut(s) 436, 539, 1437, 1453, 1459, 1582
SseBI AGGCCT 1 cut(s) 458
SspDI GGCGCC 2 cut(s) 136, 259
SspMI CTAG 4 cut(s) 606, 1089, 1509, 1777
StuI AGGCCT 1 cut(s) 458
StyD4I CCNGG 8 cut(s) 165, 176, 306, 600, 959, 1154, 1547, 1562
StyI CCWWGG 3 cut(s) 291, 972, 1719
TaaI ACNGT 4 cut(s) 87, 860, 1331, 1602
TaiI ACGT 3 cut(s) 245, 615, 779
TaqI TCGA 5 cut(s) 147, 207, 350, 369, 453
TasI AATT 6 cut(s) 436, 539, 1437, 1453, 1459, 1582
TatI WGTACW 3 cut(s) 378, 1110, 1767
TauI GCSGC 3 cut(s) 109, 137, 1749
TfiI GAWTC 3 cut(s) 949, 1183, 1492
Tru1I TTAA 2 cut(s) 1398, 1764
Tru9I TTAA 2 cut(s) 1398, 1764
TscAI CASTG 3 cut(s) 863, 1250, 1449
TseFI GTSAC 2 cut(s) 608, 1028
TseI GCWGC 3 cut(s) 407, 1469, 1706
Tsp45I GTSAC 2 cut(s) 608, 1028
TspDTI ATGAA 5 cut(s) 585, 638, 770, 1268, 1701
TspGWI ACGGA 3 cut(s) 630, 686, 1611
TspRI CASTG 3 cut(s) 863, 1250, 1449
Tth111I GACNNNGTC 1 cut(s) 476
VneI GTGCAC 1 cut(s) 881
VpaK11BI GGWCC 2 cut(s) 580, 592
XceI RCATGY 3 cut(s) 1556, 1659, 1746
XmiI GTMKAC 1 cut(s) 147
XspI CTAG 4 cut(s) 606, 1089, 1509, 1777
ZrmI AGTACT 2 cut(s) 1112, 1769
Zsp2I ATGCAT 2 cut(s) 1425, 1700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.